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1.
J Nanosci Nanotechnol ; 20(2): 731-740, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383068

RESUMO

NiO/ZnO gas-sensing nanotube materials were prepared by electrospinning. The structure and morphology of the samples were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), energydispersive X-ray detection (EDX) and Brunauer-Emmett-Teller (BET) analysis. The template, PAN (peroxyacetyl nitrate) fibers, was completely removed, as evidenced by the EDX results. The final NiO/ZnO composite materials were composed of hexagonal wurtzite ZnO and cubic NiO and exhibited hollow tubular structures. In the composites, p-n heterojunctions were formed at the interface of NiO and ZnO. The results of gas sensitivity tests showed that the incorporation of NiO considerably improved the gas sensitivity of ZnO to ethanol. When the doping ratio was 0.125 mol/mol, the composites exhibited the highest sensitivity to ethanol (100.92 at 300 °C) and showed high selectivity.

2.
Sci Rep ; 9(1): 12733, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31484967

RESUMO

The growth of winter wheat consumes a substantial amounts of water, and precipitation in most years cannot meet the water demand for the normal growth of winter wheat. The unsuitable irrigation strategies waste a large number of water resource, and the low water use efficiency has become the main factor limiting wheat yields. This research explored the effects of different cultivation managements on water consumption characteristics, water utilization efficiency, and grain yields of winter wheat. A field experiment, in which 4 cultivation managements including traditional cultivation management (T1), optimized cultivation management compared with T1 (T2), super high-yield cultivation management (T3) and optimized cultivation management compared with T3 (T4), was conducted during 2008-2010 to measure the above parameters. The results showed that different cultivation managements had significant effects on the total water consumption amounts and water source compositions. Total water consumption amounts in T1 and T3 managements were significantly higher than that in T2 and T4 managements, possibly from irrigation water. T2 and T4 managements remarkably increased the uptake and utilization of soil storage water and precipitation amounts. T3 and T1 managements increased and decreased water consumption in upper (0-40 cm) and lower (60-100 cm) soil layers, respectively, while effectively increased the consumption of storage water in middle and lower soil layers (60-100 cm) and yield water use efficiency (WUEY), precipitation water use efficiency (WUEP), soil water use efficiency (WUES), irrigation water use efficiency (WUEI), and irrigation efficiency (IE) in T4 and T2 managements were higher than those in T3 and T1, respectively. Total water consumption amounts markedly raised in T1 and T3 managements, whereas their soil storage water amounts utilization declined. T2 and T4 managements reduced irrigation water amounts and optimized the water and fertilizer supplies, resulting in significant increase in WUES and WUEI. Collectively, our results suggest that synergetic improving the water uptake and utilization of irrigation water and soil storage water can be the primary means to increase the grain yields and WUE.

3.
Echocardiography ; 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31487058

RESUMO

Unilateral absence of the pulmonary artery (UAPA) is a rare congenital cardiovascular malformation that can present as an isolated lesion or may be associated with other congenital heart malformations. Several studies have reported UAPA after birth. To our knowledge, the absence of the right pulmonary artery in the fetus has not been reported. Here, we report a rare case of fetal right pulmonary artery absence with aortic coarctation, which was confirmed by postpartum ultrasound and computed tomography angiography (CTA). Our case demonstrates that fetal echocardiography, especially the three-vessel view, is beneficial for the prenatal diagnosis of pulmonary artery malformations.

4.
Ann Thorac Surg ; 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31472132

RESUMO

BACKGROUND: This study aims to compare the mid-term outcomes of left ventricular reconstruction with those of left ventricular reconstruction plus mitral valve surgery in patients with left ventricular aneurysm due to anterior myocardial infarction and moderate mitral regurgitation. METHODS: A total of 523 patients (75 who underwent left ventricular reconstruction plus mitral valve surgery and 448 who underwent left ventricular reconstruction) with concomitant moderate mitral regurgitation were included in the study population. All-cause mortality was considered the primary endpoint. Major adverse cardiovascular and cerebrovascular events (MACCE), including death, myocardial infarction, stroke and subsequent mitral valve surgery, were considered secondary endpoints. Multivariable proportional hazards Cox regression models were used to assess the associations between groups and outcomes. In the sensitivity analysis, we excluded patients who did not undergo coronary artery bypass graft (CABG) and repeated the statistical analysis above. RESULTS: The median follow-up time among all patients was 41 months. There was no significant difference between the left ventricular reconstruction plus mitral valve surgery and the left ventricular reconstruction groups with regard to all-cause mortality (p=0.208) and MACCE (p=0.817) after adjustment for covariates. In the sensitivity analysis, there was no significant difference between the left ventricular reconstruction plus mitral valve surgery and left ventricular reconstruction groups with regard to all-cause mortality (p=0.158) and MACCE (p=0.651) after adjustment for covariates. CONCLUSIONS: The clinical outcomes of left ventricular reconstruction are comparable to those of left ventricular reconstruction plus mitral valve surgery in patients with left aneurysm and moderate mitral regurgitation.

5.
Artigo em Inglês | MEDLINE | ID: mdl-31475420

RESUMO

There is a growing understanding of the large role inorganic nanoparticles and their assemblies for Earth sciences. Complex structures from NPs are found in rocks, soils, and sea sediments but the mechanisms of their formation are poorly understood, which causes controversial conclusions about their genesis. Here we show that graphene quantum dots (GQDs), can assemble into complex structures driven by coordination interactions with metal ions commonly present in environment and serve a special role in Earth's history, such as Fe3+ and Al3+. GQDs self-assemble into mesoscale chains, sheets, supraparticles, nanoshells, and nanostars. Specific assembly patterns are determined by the effective symmetry of the GQDs when forming the coordination assemblies with the metal ions. As such, maximization of the electronic delocalization of π-orbitals of GQDs with Fe3+ leads to GQD-Fe-GQD units with D2 summery, dipolar bonding potential, and linear assemblies. Variable dihedral angles in GQD-Al-GQD structural units destroys axial symmetric relationships and results in a more isotropic bonding potential with effective S2n symmetry and, thus, the formation of supraparticles without preferred bonding axes. Taking advantage of high electron microscopy contrast of carbonatious nanostructures in respect to ceramic background, the mineralogical counterparts of GQD assemblies are found in mineraloid shungite. The examined samples of shungite contain nanocarbons with nearly identical geometrical patterns, symmetry relations, and spectroscopic signatures as the laboratory superstructures, which may reflect self-assembly processes during its formation. These findings provide insight into nanoparticle dynamics during the rock formation that can lead to mineralized structures of unexpectedly high complexity.

6.
Artigo em Inglês | MEDLINE | ID: mdl-31498586

RESUMO

Significant progress has been made to replace graphite anode materials with Li metal in next-generation Li ion batteries, called Li metal batteries (LMBs). However, the development of practical LMBs requires the suppression of Li dendrites. Owing to their ability to relax polarization, single-ion solid polymer electrolytes (SSPEs) are widely considered as an effective strategy for preventing dendrite generation. The novel SSPE membrane prepared in this work, which consists of a polymeric lithium salt modified with an electrolyte solvent, shows single-ion conducting behavior that results in the effective restriction of Li dendritic growth. The SSPE membrane delivers an ionic conductivity as high as 1.42 × 10-4 S cm-1 at room temperature. A LiFePO4 (LFP) coin cell assembled with the SSPE membrane shows excellent rate performance and outstanding cycling stability. In addition, the LFP flexible battery using the SSPE membrane exhibits good practicability and environmental adaptability.

7.
Sci Total Environ ; 697: 134088, 2019 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-31487591

RESUMO

Achieving both high yield and high nitrogen (N) use efficiency (NUE) simultaneously is a current research hotspot in crop production. To investigate approaches for achieving high yield and NUE, field experiments using N fertilizer rates of 0, 120, 180, 240, 300 and 360 kg N ha-1 were conducted to study relationships between yield, N uptake and N efficiency during three wheat growing seasons from 2013 to 2016 in three experimental sites (Shangshui, Kaifeng and Wenxian) in the Huang-Huai Plain. Yield, biomass and N concentrations of plants and soil were determined. The results indicated that increased N application would affect soil N residue and increase N2O emission, suitable N application rate (N240-N268) contributed to maintaining soil fertility and reducing N2O emission for achieving high yield, high NUE and low N2O emission. High plant N accumulation (PNA) during jointing to anthesis had the best correlation coefficient with yield and NUE compared to other growth stages, which contributed to achieving high yield and NUE simultaneously. The dry matter produced by a unit of N was defined as N productivity, such as plant N net phase productivity (PNPn) and leaf N productivity (LNP). High PNPn during jointing to anthesis was significantly related to both yield and NUE. The LNP indicator (i.e. photosynthetic N use efficiency, PNUE) in the flag showed significant correlation with both yield and NUE after booting under high PNA levels. These results suggest that PNPn and PNUE could combine high yield and high NUE under high PNA conditions. Besides, to match soil N supply to plant N demand, optimum soil nitrate N accumulation and alkali-hydrolysable N (AHN) content ranges were determined. This study provides a theoretical basis to achieve high yield, high NUE and low N2O emission for N management in wheat field production.

8.
J Pharm Biomed Anal ; 177: 112843, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31509788

RESUMO

An UHPLC method was developed for the determination of 15 prenylflavonoids from aerial parts of Epimedium grandiflorum and related species (Berberidaceae). The separation was achieved using a reverse phased column and water/acetonitrile gradient as a mobile phase at a temperature of 40°C. The developed analytical method was validated for linearity, limits of detection (LOD) and limits of quantification (LOQ), stability and repeatability. The LOD and LOQ were found to be in the range from 0.1-0.5 µg/mL and 0.3-1 µg/mL, respectively. The wavelength used for quantification with the photodiode array detector was 269 nm. The total content of 15 prenylflavonoids was 9.1-20.6 mg/g for E. grandiflorum (except for sample #2899 and #20862), 5.6-35.4 mg/g for E. brevicornu and 10.8-30.5 mg/g for E. sagittatum. Twenty dietary supplements contained in the range from 0.1 to 81.7 mg/day. The developed method is simple, rapid and especially suitable for quality assessment of E. grandiflorum and dietary supplements containing E. grandiflorum. Liquid chromatography quadrupole time-of-flight-mass spectrometry (LC-QToF) is described for the identification and confirmation of compounds in plant samples and dietary supplements. This technique is also used for chemical profiling of Epimedium samples. This method involved the use of protonated ions in the positive ion mode and deprotonated ions in the negative ion mode with extracted ion chromatogram (EIC). Chemometric analytical tools for visualizing the plant and commercial samples quality were used for discriminating between Epimedium species and dietary supplements with regards to the relative content or presence of components. A HPTLC method was also developed for the fast chemical fingerprint analysis of Epimedium species.

9.
Artigo em Inglês | MEDLINE | ID: mdl-31474104

RESUMO

Aggregation-induced emission (AIE) materials present unique solid-state fluorescence. However, there remains a challenge in the switching of fluorescence quenching/emitting of AIE materials, limiting the application in information encryption. Herein, we report a composite of tetraphenylethylene@graphene oxide (TPE@GO) with switchable microstructure and fluorescence. We choose GO as a fluorescence quencher to control the fluorescence of TPE by controlling the aggregation structure. First, TPE coating with an average thickness of about 31 nm was deposited at the GO layer surface, which is the critical thickness at which the fluorescence can be largely quenched because of the fluorescence resonance energy transfer. After spraying a mixed solvent (good and poor solvents of TPE) on TPE@GO, a blue fluorescence of TPE was emitted during the drying process. During the treatment of mixed solvents, the planar TPE coating was dissolved in THF first and then the TPE molecules aggregated into nanoparticles (an average diameter of 65 nm) in H2O during the volatilization of THF. We found that the fluorescence switching of the composite is closely related to the microstructural change of TPE between planar and granular structures, which can make the upper TPE molecules in and out of the effective quenching region of GO. This composite, along with the treatment method, was used as an invisible ink in repeated information encryption and decryption. Our work not only provides a simple strategy to switch the fluorescence of solid-state fluorescent materials but also demonstrates the potential for obtaining diverse material structures through compound solvent treatment.

10.
BMC Plant Biol ; 19(1): 394, 2019 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-31510912

RESUMO

BACKGROUND: Salinity is a major abiotic stress seriously hindering crop yield. Development and utilization of tolerant varieties is the most economical way to address soil salinity. Upland cotton is a major fiber crop and pioneer plant on saline soil and thus its genetic architecture underlying salt tolerance should be extensively explored. RESULTS: In this study, genome-wide association analysis and RNA sequencing were employed to detect salt-tolerant qualitative-trait loci (QTLs) and candidate genes in 196 upland cotton genotypes at the germination stage. Using comprehensive evaluation values of salt tolerance in four environments, we identified 33 significant single-nucleotide polymorphisms (SNPs), including 17 and 7 SNPs under at least two and four environments, respectively. The 17 stable SNPs were located within or near 98 candidate genes in 13 QTLs, including 35 genes that were functionally annotated to be involved in salt stress responses. RNA-seq analysis indicated that among the 98 candidate genes, 13 were stably differentially expressed. Furthermore, 12 of the 13 candidate genes were verified by qRT-PCR. RNA-seq analysis detected 6640, 3878, and 6462 differentially expressed genes at three sampling time points, of which 869 were shared. CONCLUSIONS: These results, including the elite cotton accessions with accurate salt tolerance evaluation, the significant SNP markers, the candidate genes, and the salt-tolerant pathways, could improve our understanding of the molecular regulatory mechanisms under salt stress tolerance and genetic manipulation for cotton improvement.

11.
Nat Prod Res ; : 1-6, 2019 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-31512512

RESUMO

Two new flavonoid glucosides, sinoflavonoidgs A (1) and B (2), along with three known analogues 3-5, were isolated from the fruits of Sinopodophyllum hexandrum. Their structures were established on the basis of extensive spectroscopic (UV, IR, HR-ESI-MS, 1H-NMR, 13C-NMR, HSQC, HMBC) and chromatographic (HPLC) analysis. The isolation of compounds 1-2 represents the first report of ring B-glucosided flavonoids from the genus Sinopodophyllum. The cytotoxic activities of all isolated compounds were evaluated in comparison with etoposide against four cell lines (MCF-7, HepG2, HeLa, KB). The antioxidant activities of all isolated compounds were examined by DPPH free radical-scavenging assay. The preliminary structure-activity relationships showed that the glycosilation of 3-methoxyquercetin at C-3' resulted in a greater decrease of cytotoxic and antioxidant activity.

12.
Opt Lett ; 44(17): 4359-4362, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31465402

RESUMO

The nonlinear frequency conversion of low-temporal-coherent light holds a variety of applications and has attracted considerable interest. However, its physical mechanism remains relatively unexplored, and the conversion efficiency and bandwidth are extremely insufficient. Here, considering the instantaneous broadband characteristics, we establish a model of second-harmonic generation (SHG) of a low-temporal-coherent pulse and reveal its differences from the coherent conditions. It is found that the second-harmonic spectrum distribution is proportional to the self-convolution of that of a fundamental wave. Because of this, we propose a method for realizing low-temporal-coherent SHG with high efficiency and broad bandwidth, and experimentally demonstrate a conversion efficiency up to 70% with a bandwidth of 3.1 THz (2.9 nm centered at 528 nm). To the best of our knowledge, this is the highest efficiency and broadest bandwidth of low-temporal-coherent SHG to date. Our research opens the door for the study of low-coherent nonlinear optical processes.

13.
Artif Cells Nanomed Biotechnol ; 47(1): 3315-3321, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31385542

RESUMO

Objective: The long intergenic non-coding RNA 01296 (LINC01296) has been reported to be overexpressed in multiple tumours. However, the role of LINC01296 in clinicopathologic and prognostic value in cancers remains completely unknown. The aim of the present meta-analysis was to comprehensively elucidate the correlation between LINC01296 with clinicopathological features and survival outcomes in tumours. Methods: Electronic databases of PubMed, Web of Science, Chinese National Knowledge Infrastructure (CNKI), and Wanfang Database were used to search relevant studies. The role of LINC01296 in cancers was evaluated by pooled hazard ratios (HRs), odds ratios (ORs) and 95% confidence intervals (CIs). Results: In total, nine studies compromising 720 participants were enrolled in this analysis. The pooled results showed increased LINC01296 expression could predict unfavourable overall survival (OS) (HR = 1.89, 95%CI = 1.47-2.43, p < .001). Additionally, elevated LINC01296 expression was correlated with clinical stage (OR = 2.95, 95%CI = 2.13-4.08, p < .001), lymph node metastasis (OR = 2.76, 95%CI = 2.00-3.81, p < .001), tumour size (OR = 2.80, 95%CI = 1.77-4.41, p < .001), and tumour differentiation (OR = 2.11, 95%CI = 1.36-3.27, p < .001) in patients with cancers. Conclusion: The results of this meta-analysis indicated LINC01296 was a novel biomarker for prognosis and clinicopathological parameters in cancers.

14.
Theranostics ; 9(16): 4597-4607, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31367243

RESUMO

During a minimally invasive tumor resection procedure, it is still a challenge to rapidly and accurately trace tiny malignant tumors in real time. Fluorescent molecular imaging is considered an efficient method of localizing tumors during surgery due to its high sensitivity and biosafety. On the basis of the fact that γ-glutamyltranspeptidase (GGT) is overexpressed in ovarian cancer, we herein designed a highly sensitive ratiometric fluorescent GGT-responsive probe Py-GSH for rapid tumor detection. Methods: The GGT response probe (Py-GSH) was constructed by using GSH group as a response group and pyrionin B as a fluorescent reporter. Py-GSH was characterized for photophysical properties, response speed and selectivity of GGT and response mechanism. The anti-interference ability of ratiometric probe Py-GSH to probe concentration and excitation power was evaluated both in vitro and in tissue. The biocompatibility and toxicity of the ratiometric probe was examined using cytoxicity test. The GGT levels in different lines of cells were determined by ratiometric fluorescence imaging and cytometry analysis. Results: The obtained probe capable to rapidly monitored GGT activity in aqueous solution with 170-fold ratio change. By ratiometric fluorescence imaging, the probe Py-GSH was also successfully used to detect high GGT activity in solid tumor tissues and small peritoneal metastatic tumors (~1 mm in diameter) in a mouse model. In particular, this probe was further used to determine whether the tissue margin following clinical ovarian cancer surgery contained tumor. Conclusion: In combination of ratiometric fluorescence probes with imaging instrument, a point-of-care imaging method was developed and may be used for surgical navigation and rapid diagnosis of tumor tissue during clinical tumor resection.

15.
Adv Mater ; : e1902899, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31456271

RESUMO

All-polymer solar cells (all-PSCs) exhibit excellent stability and readily tunable ink viscosity, and are therefore especially suitable for printing preparation of large-scale devices. At present, the efficiency of state-of-the-art all-PSCs fabricated by the spin-coating method has exceeded 11%, laying the foundation for the preparation and practical utilization of printed devices. A high power conversion efficiency (PCE) of 11.76% is achieved based on PTzBI-Si:N2200 all-PSCs processing with 2-methyltetrahydrofuran (MTHF, an environmentally friendly solvent) and preparation of active layers by slot die printing, which is the top efficient for all-PSCs. Conversely, the PCE of devices processed by high-boiling point chlorobenzene is less than 2%. Through the study of film formation kinetics, volatile solvents can freeze the morphology in a short time, and a more rigid conformation with strong intermolecular interaction combined with the solubility limit of PTzBI-Si and N2200 in MTHF results in the formation of a fibril network in the bulk heterojunction. The multilength scaled morphology ensures fast transfer of carriers and facilitates exciton separation, which boosts carrier mobility and current density, thus improving the device performance. These results are of great significance for large-scale printing fabrication of high-efficiency all-PSCs in the future.

16.
Gastric Cancer ; 2019 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-31451992

RESUMO

BACKGROUND: Long non-coding RNAs (lncRNAs) have emerged as important roles in gastric cancer (GC). However, the role of the dysregulated lncRNAs in GC remained large unknown. We investigated the clinical significance, biological function and mechanism of CTC-497E21.4 in GC. METHODS: Firstly, RTFQ-PCR was used to detect the expression of CTC-497E21.4 in GC. Furthermore, knockdown of CTC-497E21.4 was conducted to assess the effect of CTC-497E21.4 in vitro and vivo. Subcellular localization of CTC-497E21.4 was determined by nuclear plasmolysis PCR and FISH. We also predicted CTC-497E21.4 binding miRNAs and downstream target genes and evaluated its regulation of miR-22 by acting as a ceRNA. RESULT: CTC-497E21.4 was upregulated in GC tissues and GC cell lines (P < 0.05), and the expression was associated with depth of invasion, lymph node metastasis, and neurological invasion. Besides, knockdown of CTC-497E21.4 inhibited cell proliferation, invasion and promoted cell cycle arrest in vitro and inhibited tumorigenesis in vivo. Mechanistic investigations indicated that CTC-497E21.4 acted as a ceRNA for miR-22 and regulated NET1 expression. CTC-497E21.4/miR-22-3p/NET1 participated in the RhoA signaling pathway in the GC progression. CONCLUSION: CTC-497E21.4 competed with miR-22 to regulate the expression of NET1 and regulated the malignant progression of GC through RhoA signaling pathway.

18.
Philos Trans R Soc Lond B Biol Sci ; 374(1779): 20180225, 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31431171

RESUMO

Directional cell migration in dense three-dimensional (3D) environments critically depends upon shape adaptation and is impeded depending on the size and rigidity of the nucleus. Accordingly, the nucleus is primarily understood as a physical obstacle; however, its pro-migratory functions by stepwise deformation and reshaping remain unclear. Using atomic force spectroscopy, time-lapse fluorescence microscopy and shape change analysis tools, we determined the nuclear size, deformability, morphology and shape change of HT1080 fibrosarcoma cells expressing the Fucci cell cycle indicator or being pre-treated with chromatin-decondensating agent TSA. We show oscillating peak accelerations during migration through 3D collagen matrices and microdevices that occur during shape reversion of deformed nuclei (recoil), and increase with confinement. During G1 cell-cycle phase, nucleus stiffness was increased and yielded further increased speed fluctuations together with sustained cell migration rates in confinement when compared to interphase populations or to periods of intrinsic nuclear softening in the S/G2 cell-cycle phase. Likewise, nuclear softening by pharmacological chromatin decondensation or after lamin A/C depletion reduced peak oscillations in confinement. In conclusion, deformation and recoil of the stiff nucleus contributes to saltatory locomotion in dense tissues. This article is part of a discussion meeting issue 'Forces in cancer: interdisciplinary approaches in tumour mechanobiology'.

19.
FASEB J ; : fj201900965R, 2019 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-31415184

RESUMO

Schwann cells (SCs) play an essential role in nerve injury repair. A striking feature of the cellular response to peripheral nerve injury is the proliferation of SCs. Circular (circ)RNAs are enriched in the nervous system and are involved in physiologic and pathologic processes. However, the potential role of circRNAs in SC proliferation post nerve injury remains largely unknown. Using a sciatic nerve crush model, we obtained an expression profiling of circRNAs in injured sciatic nerves in rats by RNA sequencing and bioinformatics analysis, and we further identified a circRNA [circ-ankyrin repeat and in-between Ring finger (IBR) domain containing 1 (Ankib1)] involved in SC proliferation by the transfection of specific small interfering RNAs. Overexpression of circ-Ankib1, which was specifically and highly enriched in SCs, impaired SC proliferation and axon regeneration following sciatic nerve injury. Mechanistically, increased expression of DEx/H-box helicase 9 (DHX9) postinjury might contribute to the down-regulation of circ-Ankib1, which further suppressed cytochrome P450, family 26, subfamily B, polypeptide 1 expression by sponging miR-423-5p, miR-485-5p, and miR-666-3p, leading to the induction of SC proliferation and nerve regeneration. Taken together, our results reveal a crucial role for circRNAs in regulating proliferation of SCs involved in sciatic nerve regeneration; as such, circRNAs may serve as a potential therapeutic avenue for nerve injury repair.-Mao, S., Zhang, S., Zhou, S., Huang, T., Feng, W., Gu, X., Yu, B. A Schwann cell-enriched circular RNA circ-Ankib1 regulates Schwann cell proliferation following peripheral nerve injury.

20.
J Food Prot ; 82(9): 1539-1545, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31414900

RESUMO

A high concentration of biogenic amines have been reported to be hazardous for human health. This article is an analytical report on one lot to identify the changes of biogenic amines in each period of soy sauce brewing and clarify the key control point for biogenic amine production. The content of putrescine, cadaverine, spermidine, spermine, tryptamine, phenylethylamine, histamine, serotonin, tyramine, and agmatine was detected in the koji-making and fermenting process. The content of putrescine increased from 27.11 ± 1.05 to 185.86 ± 1.18 mg/kg in the koji-making process, indicating that putrescine is the main biogenic amine produced by microbes in this period. The content of tryptamine increased to the highest value of 581.77 ± 36.38 mg/L on day 24 of the fermenting process and then decreased rapidly to 81.98 ± 0.20 mg/L at the end (day 122). In addition, histamine and tyramine reached the highest values (486.91 ± 24.67 and 180.84 ± 2.32 mg/L, respectively) after 52 days of fermentation, followed by a decrease to 287.24 ± 15.00 and 144.67 ± 3.61 mg/L, respectively, at the end of the fermenting process. The samples were further characterized by the analysis of other indices, including the content of water, salt, soluble saltless solids, crude fat, total acid, amino acid nitrogen, total nitrogen, and ammonium salt. The content of soluble saltless solids decreased from 9.28 ± 0.16 to 5.30 ± 1.40 g/100 g during the first 38 days of fermentation, followed by an increase to 14.68 ± 1.12 g/100 g during the last 84 days. The content of total acid, crude fat, amino acid nitrogen, total nitrogen, and ammonium salt all increased rapidly in the early stage of the fermenting process and then slowed down.

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