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1.
Anal Chim Acta ; 1176: 338768, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34399892

RESUMO

N-doped nickel carbide spheres (N-NiCSs) were synthesised for the first time by controlling the type of surfactant, surfactant-to-Ni molar ratio, reaction temperature, and reaction time. The morphology, composition, and electrochemical behaviour of the synthesised spheres revealed that the spheres presented a large specific surface area, abundant pores, and good conductivity, with excellent electrocatalytic performance. A glassy carbon electrode-modified with N-NiCSs was used for the simultaneous identification of hydroquinone (HQ), catechol (CC), and resorcinol (RS) utilising differential pulse voltammetry. The oxidation peaks of HQ, CC, and RS were observed at 9.8, 119, and 470 mV, respectively (vs. SCE). Under optimal conditions, the oxidation peak currents of HQ, CC, and RS were linear in the concentration ranges of 0.005-100 µM, 0.05-200 µM, and 5-500 µM, respectively. The detection limits of HQ, CC, and RS were 0.00152 µM, 0.015 µM, and 0.24 µM (S/N = 3), respectively. The sensitivities of HQ, CC, and RS were 4.635, 2.069, and 0.985 µA µM-1 cm-2 (S/N = 3), respectively. The fabricated sensor was successfully used to detect HQ, CC, and RS in hair dye, whitening cream, and local tap water samples. Moreover, the sensor presented a good repeatability, reproducibility, and stability during cosmetic testing and a relatively wide linear range, an ultralow detection limit, and an ultrahigh sensitivity.


Assuntos
Cosméticos , Nitrogênio , Carbono , Níquel , Reprodutibilidade dos Testes
2.
Analyst ; 146(16): 5135-5142, 2021 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-34282821

RESUMO

Nitrogen doped carbon dots were synthesized using the hydrothermal reaction of cellulose and urea, and then carbonized in a N2 atmosphere at a high temperature to prepare N-doped carbon dots decorated with manganese oxide nanospheres (N-CMOS) formed using cetyltrimethylammonium bromide (CTAB) and MnO. The introduction of N-CMOS resulted in a large specific surface area, abundant pores, favourable conductivity and an excellent electrocatalytic performance. A glassy carbon electrode modified with N-CMOS was used for the simultaneous identification of paracetamol (AP) and p-aminophenol (PAP) utilising differential pulse voltammetry. Under optimum conditions, the electrical sensor showed a wide linear range of 0.1-100 µM for PAP and 0.1-80 µM for AP, with detection limits of 0.0456 and 0.0303 µM (S/N = 3), respectively. The sensitivities for detecting PAP and AP were calculated as 1.615 and 1.971 µA µM-1 cm-2, respectively. The sensitivity and limit of detection (LOD) meet the requirements of detection of drug impurity limits in tablets. In addition, the sensor has been successfully applied to detect PAP and AP in paracetamol tablets. The constructed sensor not only possesses a superior repeatability, reproducibility and stability, but a relatively wide linear range, and a superior detection limit and sensitivity.


Assuntos
Carbono , Nanosferas , Acetaminofen , Aminofenóis , Técnicas Eletroquímicas , Eletrodos , Limite de Detecção , Compostos de Manganês , Óxidos , Reprodutibilidade dos Testes
3.
Int J Mol Med ; 48(3)2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34278450

RESUMO

Endometrial cancer (EC) is widely known as an aggressive malignancy. Due to the limited therapeutic options and poor prognosis of patients with advanced­stage EC, there is a need to identify effective alternative treatments. Chrysin is a naturally active flavonoid (5,7­dihydroxyflavone), which has been demonstrated to exert anticancer effects and may present a novel strategy for EC treatment. However, the role of chrysin in EC remains largely unclear. The aim of the present study was to examine the anticancer effects of chrysin on EC. The results revealed that, in addition to apoptosis, chrysin increased the LC3II expression levels and markedly accelerated the autophagic flux, suggesting that chrysin induced both the autophagy and apoptosis of EC cells. Furthermore, the inhibition of autophagy by chloroquine enhanced the inhibitory effect on cell proliferation and the promotion of the chrysin­induced apoptosis of EC cells, indicating that chrysin­induced autophagy was a cytoprotective mechanism. Additionally, chrysin led to the production of intracellular reactive oxygen species (ROS). N­acetylcysteine (NAC) pretreatment significantly inhibited chrysin­induced autophagy, suggesting that ROS activated autophagy induced by chrysin in EC cells. Furthermore, the phosphorylated (p­)Akt and p­mTOR levels were significantly decreased in a concentration­dependent manner following treatment with chrysin, while NAC blocked these effects. Taken together, these findings demonstrated that chrysin­induced autophagy via the inactivation of the ROS­mediated Akt/mTOR signaling pathway in EC cells.

4.
BMC Cancer ; 21(1): 450, 2021 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-33892663

RESUMO

BACKGROUND: To explore the factors that affect the prognosis of overall survival (OS) and cancer-specific survival (CSS) of patients with stage IIIC1 cervical cancer and establish nomogram models to predict this prognosis. METHODS: Data from patients in the Surveil-lance, Epidemiology, and End Results (SEER) programme meeting the inclusion criteria were classified into a training group, and validation data were obtained from the First Affiliated Hospital of Anhui Medical University from 2010 to 2019. The incidence, Kaplan-Meier curves, OS and CSS of patients with stage IIIC1 cervical cancer in the training group were evaluated. Nomograms were established according to the results of univariate and multivariate Cox regression models. Harrell's C-index, calibration plots, receiver operating characteristic (ROC) curves and decision-curve analysis (DCA) were calculated to validate the prediction models. RESULTS: The incidence of pelvic lymph node metastasis, a high-risk factor for the prognosis of cervical cancer, decreased slightly over time. Eight independent prognostic variables were identified for OS, including age, race, marriage status, histology, extension range, tumour size, radiotherapy and surgery, but only seven were identified for CSS, with marriage status excluded. Nomograms of OS and CSS were established based on the results. The C-indexes for the nomograms of OS and CSS were 0.687 and 0.692, respectively, using random sampling of SEER data sets and 0.701 and 0.735, respectively, using random sampling of external data sets. The AUCs for the nomogram of OS were 0.708 and 0.705 for the SEER data sets and 0.750 and 0.750 for the external data sets, respectively. In addition, AUCs of 0.707 and 0.709 were obtained for the nomogram of CSS when validated using SEER data sets, and 0.788 and 0.785 when validated using external data sets. Calibration plots for the nomograms were almost identical to the actual observations. The DCA also indicated the value of the two models. CONCLUSIONS: Eight independent prognostic variables were identified for OS. The same factors predicted CSS, with the exception of the marriage status. Both OS and CSS nomograms had good predictive and clinical application value after validation. Notably, tumour size had the largest contribution to the OS and CSS nomograms.


Assuntos
Nomogramas , Neoplasias do Colo do Útero/mortalidade , Neoplasias do Colo do Útero/patologia , Adulto , Fatores Etários , Idoso , Área Sob a Curva , Técnicas de Apoio para a Decisão , Feminino , Humanos , Estimativa de Kaplan-Meier , Linfonodos/patologia , Metástase Linfática , Estado Civil , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Pelve , Modelos de Riscos Proporcionais , Estudos Retrospectivos , Fatores de Risco , Programa de SEER , Carga Tumoral , Neoplasias do Colo do Útero/etnologia , Neoplasias do Colo do Útero/terapia
5.
Chemosphere ; 269: 128677, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33657748

RESUMO

Toxicity of microplastics (MPs) in granular form to aquatic animals has been frequently tested, whereas the effects of fibrous MPs remain further explored. In this study, the effects of polyethylene terephthalate granular particles (p-PET, approximately 150 µm in diameter) and fibers (f-PET, approximately 3-5 mm in length and 20 µm in diameter) on the development of zebrafish embryos and their joint effects with cadmium (Cd) were compared. p-PET and f-PET accelerated the velocities of blood flow and heart rate and inhibited hatching in zebrafish embryos because of their barrier effects on the channels in the embryonic chorion and enhanced the mechanical strength of the chorion. The Cd content in the chorion increased by p-PET due to the adsorption of p-PET on the chorion. By contrast, more f-PET dissociated in culture medium and resulted in low Cd content in the chorion. Given that chorion can effectively block p-PET and f-PET, the Cd accumulation in eggs significantly decreased (p < 0.05) under p-PET/f-PET and Cd combined treatment because of the reduction in the bioavailability of Cd. Therefore, p-PET and f-PET decreased the toxicities of Cd on all the target endpoints in this study, and the detoxification effect of f-PET at 72 hpf was more significant than that of p-PET. These results suggest that the toxicity induced by MPs might be form-related.


Assuntos
Microplásticos , Poluentes Químicos da Água , Animais , Cádmio/toxicidade , Córion , Embrião não Mamífero , Plásticos/toxicidade , Poluentes Químicos da Água/toxicidade , Peixe-Zebra
6.
J Food Sci ; 86(3): 779-791, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33598925

RESUMO

Pu-erh tea is believed to be a beneficial beverage for health due to its many kinds of pharmacological effects. Nevertheless, detailed information related to differences in metabolites of Pu-erh raw tea from different geographical origins remains scarce. In this study, 43 elements were found in water-soluble components of Pu-erh raw tea by highly sensitive ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (U-HPLC/Q-TOF-MS). The characteristic groups of 29 metabolites from nondestructive proton nuclear magnetic resonance (1 H-NMR) spectroscopy were assigned. The variables contributed largely to the origin classification, mainly including valine, threonine, chlorogenic acid, quinic acid, epiafzelechin, and gallic acid ester, were screened out by sparse partial least squares discriminant analysis (sPLS-DA) method. This study provided a feasible and rapid technique for distinguishing Pu-erh tea from different areas by 1 H-NMR combined with sPLS-DA.


Assuntos
Camellia sinensis/classificação , Chá/classificação , Camellia sinensis/química , Catequina , Cromatografia Líquida de Alta Pressão , Análise Discriminante , Flavonoides/análise , Análise dos Mínimos Quadrados , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Extratos Vegetais/química , Chá/química
8.
Angiogenesis ; 24(3): 489-504, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-33400016

RESUMO

Ocular neovascularization is the leading cause of vision impairment in a variety of ocular diseases, such as age-related macular degeneration and retinopathy of prematurity. Emerging studies have suggested that the yes-associated protein (YAP), a downstream effector of the Hippo pathway, is involved in the pathological angiogenesis, but the mechanism are largely unknown. Here, we demonstrated that hypoxic treatment triggered YAP expression and nuclear translocation in human umbilical vein endothelial cells (HUVECs). YAP acted as a transcriptional co-activator working together with transcriptional enhancer activator domain 1 (TEAD1) to binds the promoter of the key glycolytic regulator 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase3 (PFKFB3), and thereby increases PFKFB3 expression. Moreover, silencing of YAP inhibited glycolysis as well as proliferation, migration, sprouting and tube formation of HUVECs under hypoxia, all of which could be reversed by enforced expression of PFKFB3. Finally, our animal study also showed that intravitreal injection of small interfering RNA of YAP or PFKFB3 dramatically suppressed the neovascular growth in mouse models of choroidal neovascularization and oxygen-induced retinopathy. These findings provide new insights into a previously unrecognized effect of YAP on endothelial glycolysis and highlight the potential of targeting YAP/PFKFB3 axis in the treatment of ocular neovascularization.

9.
Carbohydr Polym ; 253: 117216, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33278980

RESUMO

Uveitis is one of the most popular blind-causing eye diseases worldwide. Adalimumab (ADA) is used for the uveitis treatment through systemic or intravitreal injection at the expense of systemic side effects and increased medical risks. Although eye drops, a non-invasive topical treatment, could be a potential strategy to reduce side effects, it remains challenging to apply due to limited bioavailability mainly linked to poor retention time and permeation capacity for eye biological barriers. Here, we reported hydrogel eye drops composed of low-deacetylated chitosan and ß-glycerophosphate as an ADA carrier and tested its toxicity, tolerability, intraocular permeability, and efficacy of non-invasive treatment for uveitis. It's found the ADA-loaded hydrogel eye drops were more efficient than free ADA both in permeation rate and clinical efficacy for uveitis, Overall, this study provides a friendly non-invasive strategy to improve drug permeation rate and uveitis treatment efficacy, which may be valuable to clinically ophthalmic medication.


Assuntos
Adalimumab/administração & dosagem , Administração Oftálmica , Anti-Inflamatórios/administração & dosagem , Portadores de Fármacos/administração & dosagem , Hidrogéis/administração & dosagem , Soluções Oftálmicas/administração & dosagem , Uveíte/tratamento farmacológico , Adalimumab/farmacocinética , Animais , Anti-Inflamatórios/farmacocinética , Materiais Biocompatíveis/administração & dosagem , Materiais Biocompatíveis/química , Disponibilidade Biológica , Quitosana , Portadores de Fármacos/química , Liberação Controlada de Fármacos , Feminino , Glicerofosfatos , Hidrogéis/química , Masculino , Soluções Oftálmicas/química , Permeabilidade/efeitos dos fármacos , Coelhos , Ratos , Ratos Sprague-Dawley , Resultado do Tratamento
10.
Molecules ; 25(24)2020 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-33302335

RESUMO

Herein, we sought to evaluate the contribution of the 1,3,5-triazine ring through the metformin cyclization unit to the biological activity of magnolol and honokiol-conjugates. One of the phenolic OH groups of magnolol or honokiol was replaced by a 1,3,5-triazine ring to further explore their synthesis and medicinal versatility. In this study, a robust procedure of three steps was adopted for the synthesis of magnolol and honokiol derivatives by alkylation of potassium carbonate with a 1,3,5-triazine ring. To our knowledge, this is the first report to connect one of the phenolic OH positions of magnolol or honokiol to a 1,3,5-triazine ring cyclized by metformin. The structural characterization of three new compounds was carried out via spectroscopic techniques, i.e., 13C NMR, 1H NMR, and HRMS. Surprisingly, these compounds showed no cytotoxicity against RAW 264.7 macrophages but significantly inhibited the proliferation of MCF-7 (human breast cancer cells), HepG2 (human hepatoma cells), A549 (human lung carcinoma cells), and BxPC-3 (human pancreatic carcinoma cells) tumor cell lines. Furthermore, the compounds also significantly inhibited the release of inflammatory cytokines, including nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-1ß (IL-1ß) in the lipopolysaccharide (LPS)-activated mouse cells (RAW 264.7). Among them, compound 2 demonstrated promising broad-spectrum antiproliferative potential with half inhibitory concentration (IC50) values ranging from 5.57 to 8.74 µM and it significantly decreased caspase-3 and Bcl-2 expression in HepG2 cells. These interesting findings show that derivatization of magnolol and honokiol with 1,3,5-triazine affects and modulates their biological properties.


Assuntos
Compostos de Bifenilo/síntese química , Compostos de Bifenilo/farmacologia , Técnicas de Química Sintética , Lignanas/síntese química , Lignanas/farmacologia , Metformina/química , Triazinas/química , Animais , Antineoplásicos/síntese química , Antineoplásicos/química , Antineoplásicos/farmacologia , Compostos de Bifenilo/química , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Ciclização , Citocinas/biossíntese , Regulação da Expressão Gênica , Humanos , Lignanas/química , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Macrófagos/metabolismo , Camundongos , Estrutura Molecular , Células RAW 264.7 , Relação Estrutura-Atividade
11.
ACS Nano ; 14(11): 15403-15416, 2020 11 24.
Artigo em Inglês | MEDLINE | ID: mdl-33174744

RESUMO

Human choroidal melanoma (HCM) is one of the most common primary intraocular tumors and easily provokes liver metastases owing to the lack of sensitive and noninvasive therapeutic methods. Concerning the imaging diagnostics and therapeutic predicaments for choroidal melanoma, we designed microenvironment-triggered degradable hydrogels (RENP-ICG@PNIPAM:Dox-FA) based on ultrasmall (<5 nm) rare-earth nanoparticles (RENPs) with enhanced NIR-II luminescence. The ultrasmall diameter can significantly enhance the NIR-II luminescence performance of RENPs. RENPs were encapsulated by a dual-response PNIPAM hydrogel, which could release drug by responding to heat energy and glutathione under the tumor microenvironment. The in vitro/in vivo NIR-II imaging detection and antitumor activity were also compared systematically after different treatment conditions on ocular choroidal melanoma-1 cells and tumor-bearing mice, respectively. Besides, the degradability of the hydrogel composites under physiological conditions could be conducive to enhance the photothermal-chemotherapeutic effect and alleviate long-term biological toxicity. Our work on the microenvironment-triggered hydrogels with enhanced NIR imaging and easy metabolism may provide a promising strategy for sensitive and noninvasive imaging and phototherapy in ocular tumors.


Assuntos
Melanoma , Nanopartículas , Animais , Linhagem Celular Tumoral , Doxorrubicina , Hidrogéis , Melanoma/diagnóstico por imagem , Melanoma/tratamento farmacológico , Camundongos , Fototerapia , Microambiente Tumoral
12.
Artigo em Inglês | MEDLINE | ID: mdl-33246279

RESUMO

Sandalwood is one of the most valuable woods in the world. However, today's counterfeits are widespread, it is difficult to distinguish authenticity. In this paper, similar genus (Dalbergia and Pterocarpus) and confused species (Gluta sp.) of sandalwood were quickly and efficiently identified. Rapid identification model based on 1H NMR and decision tree (DT) algorithm was firstly developed for the identification of sandalwood, and the accuracy was improved by introducing the AdaBoost algorithm. The accuracy of the final model was above 95%. And the feature components between different species of sandalwood were further explored using UHPLC-QTOFMS and NMR spectrometry. The results showed that 183 compounds were identified, among which 99 were known components, 84 were unknown components. The 1H NMR and 13C NMR signals of 505 samples were assigned, among them, 14 compounds were attributed, characteristic chemical shift intervals with great differences in the model were analysed. Furthermore, the fragmentation pattern of different compounds from sandalwood, in both positive and negative ion ESI modes, was summarized. The results showed a potential and rapid tool based on DT, NMR spectroscopy and UHPLC-QTOFMS, which had performed great potential for rapid identification and feature analysis of sandalwood.


Assuntos
Algoritmos , Cromatografia Líquida de Alta Pressão/métodos , Espectroscopia de Ressonância Magnética/métodos , Espectrometria de Massas/métodos , Santalum/química , Árvores de Decisões , Flavonoides/análise , Flavonoides/química , Glicosídeos/análise , Glicosídeos/química
13.
Bioorg Med Chem Lett ; 30(19): 127457, 2020 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-32755681

RESUMO

PI3Kδ in B cells mediates antigen receptor signaling and promote neutrophil chemotaxis. The activation of PI3Kδ can cause mast cell maturation and degranulation, myeloid cell dysfunction, and cytokine release. As a key signal molecule, PI3Kδ interacts with the lipid binding domain of a variety of cellular proteins as a secondary messenger, ultimately affecting a series of significant cellular pathways in disease pathology. Therefore, many research organizations and pharmaceutical companies have studied it to develop effectively selective PI3Kδ inhibitors as therapeutics. This review summarizes research advances in varying chemical classes of selective PI3Kδ inhibitors and the structure-activity relationship, and it mainly focuses on the propeller- versus flat-type class of inhibitors.


Assuntos
Classe I de Fosfatidilinositol 3-Quinases/antagonistas & inibidores , Compostos Heterocíclicos/uso terapêutico , Inibidores de Proteínas Quinases/uso terapêutico , Antineoplásicos/química , Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Doenças Autoimunes/tratamento farmacológico , Linhagem Celular Tumoral , Ensaios Clínicos como Assunto , Compostos Heterocíclicos/síntese química , Compostos Heterocíclicos/farmacologia , Humanos , Pneumopatias Obstrutivas/tratamento farmacológico , Estrutura Molecular , Neoplasias/tratamento farmacológico , Inibidores de Proteínas Quinases/química , Inibidores de Proteínas Quinases/farmacologia , Relação Estrutura-Atividade
14.
Neuropharmacology ; 177: 108237, 2020 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-32710978

RESUMO

Major depressive disorder (MDD) is a severe mental disorder with a high disability rate worldwide. Selective serotonin reuptake inhibitors (SSRIs) and serotonin and norepinephrine reuptake inhibitors (SNRIs) are the most common agents for antidepressant use. SSRIs and SNRIs are believed to achieve antidepressant effects through the activation of serotonergic or noradrenergic systems. However, whether the dopaminergic system is involved remains unclear. In our study, a genetically encoded dopamine sensor and in vivo fiber photometry recordings were used to measure the dopamine concentrations in the medial prefrontal cortex (mPFC) and nucleus accumbens (NAc) after acute intraperitoneal injection of SSRIs or SNRIs. Combined with the behavioral tests, we found that SNRIs increased dopamine concentrations in both the mPFC and the NAc and showed faster antidepressant effects than SSRIs. To verify the enhanced dopamine levels induce the faster antidepressant effects of SNRIs, we employed dopamine receptor antagonists to specifically block the dopaminergic function. The results showed that the faster antidepressant effects of SNRIs were weakened by the dopamine receptor antagonists. Altogether, our study reveals that SNRIs achieve faster antidepressant effects than SSRIs by elevating the dopamine concentrations in the mPFC and the NAc. Our work proposes further mechanisms for the first-line antidepressants, which provides more basis for clinical treatments. This article is part of the special issue on Stress, Addiction and Plasticity.


Assuntos
Antidepressivos/administração & dosagem , Depressão/metabolismo , Dopamina/metabolismo , Prosencéfalo/metabolismo , Inibidores de Captação de Serotonina/administração & dosagem , Inibidores da Recaptação de Serotonina e Norepinefrina/administração & dosagem , Animais , Depressão/tratamento farmacológico , Depressão/psicologia , Dopamina/análise , Feminino , Injeções Intraperitoneais , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Fotometria/métodos , Prosencéfalo/química , Prosencéfalo/efeitos dos fármacos , Restrição Física/efeitos adversos , Restrição Física/psicologia , Resultado do Tratamento
15.
Nanoscale ; 12(28): 15201-15208, 2020 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-32638799

RESUMO

We present a new strategy for the biomimetic preparation of integrated photoactive complexes consisting of light harvesting and electron separation/transfer components via the hierarchical assembly of porphyrin and platinum nanoparticles on the surface of short-peptide self-assembled structures. The complexes can catalyze the conversion of visible light energy into chemical energy in the absence of an electron mediator and store it as nicotinamide adenine dinucleotide (NADH). This provides a promising step towards artificial photosystems through precisely controlled interactions of light-capturing agents, electron separators and biomolecules.


Assuntos
Nanopartículas Metálicas , Porfirinas , Transporte de Elétrons , Peptídeos , Platina
16.
Biomed Res Int ; 2020: 1216926, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32566651

RESUMO

Although SDF-1/CXCR7 plays an important role in angiogenesis, the function and the pathway of the SDF-1/CXCR7 axis might depend on the cell type or tissue origin and not fully understood. In this study, we investigated the effect of CXCR7 in SDF-1-induced proliferation, migration, apoptosis, tube formation, and endothelial-to-mesenchymal transition (EndMT) of human umbilical vein endothelial cells (HUVECs), and the potential pathway of SDF-1/CXCR7. We confirmed that the silencing of CXCR7 inhibited the proliferation of HUVECs and contributed the apoptosis, while overexpressed CXCR7 increased SDF-1-induced HUVECs migration and tube formation. However, upregulated CXCR7 inhibited the expression of α-SMA, suggesting that CXCR7 might attenuate EndMT. In addition, overexpressed CXCR7 activated AKT and ERK signaling pathways but suppressed Wnt/ß-catenin pathways in HUVECs. The inhibition of Wnt/ß-catenin pathways decreased the expression of α-SMA. Altogether, these results suggest that CXCR7 might inhibit fibrosis via Wnt/ß-catenin pathways during the process of angiogenesis.


Assuntos
Fibrose/metabolismo , Neovascularização Fisiológica/fisiologia , Receptores CXCR , Via de Sinalização Wnt/fisiologia , beta Catenina/metabolismo , Movimento Celular/genética , Células Cultivadas , Quimiocina CXCL12/metabolismo , Células Endoteliais da Veia Umbilical Humana , Humanos , Receptores CXCR/genética , Receptores CXCR/metabolismo , Proteínas Wnt/metabolismo
18.
J Sep Sci ; 43(13): 2728-2736, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32281256

RESUMO

Biogenic amines have been reported in many foods such as fish, meat, and soy sauce. The consumption of foods containing high concentrations of biogenic amines has been associated with health hazards. In this study, a green and efficient method using supercritical fluid chromatography coupled with single quadrupole mass spectrometry was developed for determination of biogenic amines in soy sauce. The chromatographic and mass spectrometry conditions were systematically optimized in terms of selectivity and peak shape. Nine biogenic amines were well separated within 25 min on a Cosmosil 5HP column using 5% (v/v) water and 0.2% (v/v) ammonia solution in methanol as mobile phase additives at a backpressure of 120 bar and temperature of 40°C. The established method was fully validated regarding the linearity, sensitivity, precision, and accuracy. The limits of detection and limits of quantification ranged from 0.03 to 10.50 µg/mL and 0.10 to 23.1 µg/mL, respectively. The relative standard deviations for intra- and interday precisions were all lower than 9.36% and the recoveries ranged from 75.82 to 99.63% and 80.10 to 99.89% for two levels of standards spiked in soy sauce, respectively. Finally, the established method was successfully applied to the quantitative analysis of biogenic amines in soy sauce.


Assuntos
Aminas/análise , Cromatografia com Fluido Supercrítico , Alimentos de Soja/análise , Espectrometria de Massas , Estrutura Molecular
19.
Cell Biol Int ; 44(7): 1416-1425, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32190949

RESUMO

Nuclear-cytoplasmic transport is necessary for the biological function of nuclear proteins. The mechanism underlying this process is very complex and has been a subject of intense research. Yes-associated protein (YAP), a Hippo signaling pathway effector, localizes to both the cytoplasm and the nucleus and can influence cell proliferation, stem cell status, and tissue homeostasis. Recent studies have focused on the significance of YAP distribution between the nucleus and the cytoplasm in disease, but it remains unclear how this dynamic process is regulated. In this review, we discuss YAP nuclear-cytoplasmic transport under different physiological and pathological conditions in terms of mechanical signaling, protein modification, and metabolism. Understanding the mechanisms underlying nuclear-cytoplasmic YAP transport mechanism under different physiological and pathological conditions may help identify important targets for disease treatment.


Assuntos
Proteínas de Ciclo Celular/metabolismo , Citoplasma/metabolismo , Proteínas Nucleares/metabolismo , Processamento de Proteína Pós-Traducional/fisiologia , Núcleo Celular/metabolismo , Proliferação de Células/fisiologia , Humanos
20.
Clin Exp Pharmacol Physiol ; 47(8): 1382-1392, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32155673

RESUMO

In this study, we investigated the lipid metabolism regulatory activity of a novel metformin derivative (MD568) and its potential mechanism of action in obese rats with type 2 diabetes mellitus (T2 DM). Previous gene chip analysis of 3T3-L1 cells have shown that MD568 regulates the transcription of genes involved in the peroxisome proliferator-activated receptor (PPAR) signalling pathway, fatty acid metabolism, and glycerolipid metabolism. In this study, obese T2 DM rats were treated with MD568 (200 mg/kg) for 8 weeks. Results showed that MD568 significantly reduced the body weight gain, plasma glucose, insulin, total cholesterol, triglyceride, and low-density lipoprotein cholesterol levels. MD568 treatment also improved the insulin resistance of obese T2 DM model rats. In particular, in white adipose tissue, MD568 inhibited the excessive volume increment of adipose cells by down-regulating the protein levels of CCAAT/enhancer-binding protein-α (C/EBP-α) and PPAR-γ, as well as the transcription of their target lipid metabolism-related genes. In the liver, MD568 inhibited hepatic fatty lesions and interfered with hepatic gluconeogenesis by regulating the expression of lipid metabolism-related genes and glycogen-related kinases. In conclusion, our results suggest that the newly synthesized MD568 affects the maintenance of lipid homeostasis in obese type 2 diabetic rats.


Assuntos
Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/metabolismo , Metabolismo dos Lipídeos/efeitos dos fármacos , Metformina/química , Metformina/farmacologia , Obesidade/complicações , Animais , Masculino , Ratos
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