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J Zhejiang Univ Sci B ; 16(6): 524-32, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26055914


The energy homeostasis-associated (Enho) gene encodes a secreted protein, adropin, which regulates the expression of hepatic lipogenic genes and adipose tissue peroxisome proliferator-activated receptor γ, a major regulator of lipogenesis. In the present study, the porcine (Sus scrofa) homologue of the Enho gene, which was named pEnho, was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) using oligonucleotide primers derived from in silico sequences. The gene sequence was submitted into the GenBank of NCBI, and the access number is GQ414763. The pEnho encodes a protein of 76 amino acids which shows 75% similarity to Homo sapiens adropin. The expression profile of pEnho in tissues (liver, muscle, anterior jejunum, posterior jejunum, and ileum) was determined by quantitative real-time RT-PCR. pEnho was localized on porcine chromosome 10 and no introns were found. In conclusion, pEnho was cloned and analysed with the aim of increasing knowledge about glucose and lipid metabolism in piglets and helping to promote the health and growth of piglets through adropin regulation.

Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Suínos/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , Dados de Sequência Molecular , Especificidade de Órgãos , Suínos/genética , Distribuição Tecidual
Obes Facts ; 8(2): 87-100, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25791341


BACKGROUND: The Chinese have been undergone rapid transition to a high-fat diet-consuming lifestyle, while monosodium L-glutamate (MSG) is widely used as a daily food additive. It has been reported that fat alters the composition of intestinal microbiota. However, little information is available on the effects of oral MSG on intestinal microbiota, and no study was done focusing on the interaction effect of fat and MSG with respect to intestinal microbiota. The present study thus aimed to determine the effects of MSG and/or fat on intestinal microbiota, and also to identify possible interactions between these two nutrients. METHODS: Four iso-nitrogenous and iso-caloric diets were provided to growing pigs. The microbiota from jejunum, ileum, cecum, and colon were analyzed. RESULTS: Our results show that both MSG and fat clearly increased the intestinal microbiota diversity. MSG and fat modified the composition of intestinal microbiota, particularly in the colon. Both MSG and fat promoted the colonization of microbes related to energy extraction in gastrointestinal tract via different ways. MSG promoted the colonization of Faecalibacterium prausnitzii and Roseburia, while fat increased the percentage of Prevotella in colon and other intestinal segments. CONCLUSION: Our results will help to understand how individual or combined dietary changes modify the microbiota composition to prevent obesity.

Bactérias/efeitos dos fármacos , Dieta , Gorduras na Dieta/farmacologia , Comportamento Alimentar , Microbioma Gastrointestinal/efeitos dos fármacos , Intestinos/efeitos dos fármacos , Glutamato de Sódio/farmacologia , Animais , Bactérias/crescimento & desenvolvimento , China , Dieta Hiperlipídica , Feminino , Aditivos Alimentares/farmacologia , Intestinos/microbiologia , Masculino , Obesidade/microbiologia , Obesidade/prevenção & controle , Suínos
Appl Biochem Biotechnol ; 166(3): 640-51, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22109740


Bovine lactoferrampin (LFA) and bovine lactoferricin (LFC) are two antimicrobial peptides located in the N(1) domain of bovine lactoferrin. The bactericidal activity of the fused peptide LFA-LFC is stronger than that of either LFA or LFC. The high cost of peptide production from either native digestion or chemical synthesis limits the clinical application of antimicrobial peptides. The expression of recombinant peptides in yeast may be an effective alternative. In the current study, the expression, purification, and antibacterial activity of LFA-LFC using the Pichia pastoris expression system are reported. The linearized expression vector pPICZaA-LFA-LFC was transformed into P. pastoris KM71 by electroporation, and positive colonies harboring the target genes were screened out and used for fermentation. The recombinant LFA-LFC peptide was purified via two-step column chromatography and identified by tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The results indicate that P. pastoris is a suitable system for secreting LFA-LFC. The fermentation supernate and the purified LFA-LFC show high antimicrobial activities. The current study is the first to report on the expression and purification of LFA-LFC in P. pastoris and may have potential practical applications in microbial peptide production.

Antibacterianos/farmacologia , Peptídeos Catiônicos Antimicrobianos/genética , Escherichia coli/efeitos dos fármacos , Lactoferrina/genética , Fragmentos de Peptídeos/genética , Pichia/genética , Proteínas Recombinantes de Fusão/genética , Sequência de Aminoácidos , Animais , Antibacterianos/química , Peptídeos Catiônicos Antimicrobianos/isolamento & purificação , Peptídeos Catiônicos Antimicrobianos/farmacologia , Bovinos , Clonagem Molecular , Eletroforese em Gel de Poliacrilamida , Eletroporação , Escherichia coli/crescimento & desenvolvimento , Lactoferrina/química , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Fragmentos de Peptídeos/química , Pichia/metabolismo , Plasmídeos , Proteínas Recombinantes de Fusão/isolamento & purificação , Proteínas Recombinantes de Fusão/farmacologia , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Transfecção
Front Biosci (Schol Ed) ; 3: 286-97, 2011 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-21196377


L-Tryptophan is a nutritionally essential amino acid for monogastric animals and preweaning ruminants because it cannot be synthesized in the body. Besides serving as a building block for proteins, tryptophan is a critical nutrient for the functions of nervous and immune systems. Over the past decades, much attention has been directed to study the role of tryptophan as a limiting amino acid in mammalian and avian nutrition. However, emerging evidence from recent studies shows that tryptophan and its metabolites (e.g., serotonin (5-hydroxytryptamine, 5-HT) and melatonin)) can regulate feed intake, reproduction, immunity, neurological function, and anti-stress responses. Additionally, tryptophan may modulate gene expression and nutrient metabolism to impact whole-body homeostasis in organisms. Thus, adequate intake of this amino acid from the diet is crucial for growth, development, and health of animals and humans.

Fenômenos Fisiológicos da Nutrição Animal/fisiologia , Regulação da Expressão Gênica/fisiologia , Homeostase/fisiologia , Sistema Nervoso/metabolismo , Triptofano/imunologia , Triptofano/metabolismo , Animais , Humanos , Melatonina/metabolismo , Niacina/metabolismo , Necessidades Nutricionais , Serotonina/metabolismo , Triptofano/administração & dosagem