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1.
Artif Cells Nanomed Biotechnol ; 48(1): 435-442, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31916466

RESUMO

Non-coding RNAs play an important role in the pathogenesis of prostate cancer (PC). This study aims to characterize the role of GAS5 rs145204276 and HOTAIR rs4759314 polymorphisms in the pathogenesis of PC. Both INS allele of GAS5 rs145204276 and A allele of HOTAIR rs4759314 were identified to increase the survival of PC patients. And patients carrying DEL/DEL + AG genotypes tend to present higher levels of HMGB1, GAS5, HOTAIR and lower levels of miR-1284 and miR-22. In addition, the transcription activity of GAS5 promoter was increased by the deletion allele of rs145204276 polymorphism, while the G allele of rs4759314 polymorphism increased the transcription activity of HOTAIR promoter. GAS5 and HOTAIR could bind to miR-1284 and miR-22, respectively, while miR-1284 and miR-22 could bind to the 3'UTR of HMGB1. Compared with the control group, the expressions of miR-1284 or miR-22 were decreased with the presence of GAS5 or HOTAIR, and the expression of HMGB1 was the highest in the GAS5 + HOTAIR group. In summary, the findings of this study demonstrated that both GAS5 rs145204276 and HOTAIR rs4759314 polymorphisms could affect the prognosis of PC by modulating the expression of HMGB1 via modulating the GAS5/miR-1284/HMGB1 and HOTAIR/miR-22/HMGB1 signalling pathways.

2.
BMJ Open ; 9(10): e024595, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31619412

RESUMO

OBJECTIVE: Inconsistent findings in regard to association between different concentrations of vitamin D, calcium or their combination and the risk of fracture have been reported during the past decade in community-dwelling older people. This study was designed to compare the fracture risk using different concentrations of vitamin D, calcium or their combination. DESIGN: A systematic review and network meta-analysis. DATA SOURCES: Randomised controlled trials in PubMed, Cochrane library and Embase databases were systematically searched from the inception dates to 31 December 2017. OUTCOMES: Total fracture was defined as the primary outcome. Secondary outcomes were hip fracture and vertebral fracture. Due to the consistency of the original studies, a consistency model was adopted. RESULTS: A total of 25 randomised controlled trials involving 43 510 participants fulfilled the inclusion criteria. There was no evidence that the risk of total fracture was reduced using different concentrations of vitamin D, calcium or their combination compared with placebo or no treatment. No significant associations were found between calcium, vitamin D, or combined calcium and vitamin D supplements and the incidence of hip or vertebral fractures. CONCLUSIONS: The use of supplements that included calcium, vitamin D or both was not found to be better than placebo or no treatment in terms of risk of fractures among community-dwelling older adults. It means the routine use of these supplements in community-dwelling older people should be treated more carefully. PROSPERO REGISTRATION NUMBER: CRD42017079624.

3.
Eur J Pharmacol ; 861: 172612, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31421088

RESUMO

Denervation caused by sciatic nerve injury has brought great harm to the patients, especially denervation-induced muscle atrophy. The body stress produces a large number of Schwann cells when the sciatic nerve is injured, and the cells secrete some cytokines including ciliary neurotrophic factor (CNTF) that not only play a role in promoting the repair of sciatic nerve, but also maintain the normal physiological function of the muscles surrounding the damaged nerves. CNTF upregulates janus kinase 2 (JAK2) and signal transducers and activators of transcription 3 (STAT3) signals in myoblasts, and consequently accelerates the proliferation and differentiation of myoblasts. This effect on myoblasts is the most effective way to relieve muscle atrophy. Therefore, increasing CNTF is a promising direction to improve muscle atrophy. In the present study, an oleanolic acid derivative, HA-19, increased the proliferation of Schwann cells, and elevated CNTF production of the cells. HA-19 up-regulated the phosphorylation of JAK2 and STAT3 not only by directly acting on myoblasts, but also by increasing the secretion of CNTF of Schwann cells; and consequently, promoted the proliferation and differentiation of myoblasts. In denervation-induced muscle atrophy mice model, treatment with HA-19 significantly increased the weights of tibialis anterior (TA), gastrocnemius (Gastroc.), extensor digitorum longus (EDL), soleus and quadriceps (Quad.) under atrophied state. And, very interestingly, these muscles under normal condition were also strengthened by HA-19. Our finding demonstrated that HA-19 has a great potential as a lead compound for the drug discovery of anti-denervation-induced muscle atrophy.

4.
IEEE Trans Image Process ; 28(11): 5596-5609, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31170074

RESUMO

With efficient appearance learning models, discriminative correlation filter (DCF) has been proven to be very successful in recent video object tracking benchmarks and competitions. However, the existing DCF paradigm suffers from two major issues, i.e., spatial boundary effect and temporal filter degradation. To mitigate these challenges, we propose a new DCF-based tracking method. The key innovations of the proposed method include adaptive spatial feature selection and temporal consistent constraints, with which the new tracker enables joint spatial-temporal filter learning in a lower dimensional discriminative manifold. More specifically, we apply structured spatial sparsity constraints to multi-channel filters. Consequently, the process of learning spatial filters can be approximated by the lasso regularization. To encourage temporal consistency, the filter model is restricted to lie around its historical value and updated locally to preserve the global structure in the manifold. Last, a unified optimization framework is proposed to jointly select temporal consistency preserving spatial features and learn discriminative filters with the augmented Lagrangian method. Qualitative and quantitative evaluations have been conducted on a number of well-known benchmarking datasets such as OTB2013, OTB50, OTB100, Temple-Colour, UAV123, and VOT2018. The experimental results demonstrate the superiority of the proposed method over the state-of-the-art approaches.

5.
Toxicol Appl Pharmacol ; 378: 114625, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31201822

RESUMO

Muscle atrophy refers to a decrease in the size of muscles in the body, occurs in certain muscles with inactivity in many diseases and lacks effective therapies up to date. Natural products still play an important role in drug discovery. In the present study, derivatives of a natural product, oleanolic acid, were screened with myoblast differentiation and myotube atrophy assays, respectively. Results revealed that one of the derivatives, HA-19 showed the most potent anti-muscle atrophy activity, and was used for further studies. We demonstrated that HA-19 led to the increase of the protein synthesis by activating mechanistic target of rapamycin complex 1 (mTORC1)/p70 S6K pathways, and also enhanced myoblast proliferation and terminal differentiation via up-regulating of the myogenic transcription factors Pax7, MyoD and Myogenin. The interesting thing was that HA-19 also suppressed protein degradation to prevent myotube atrophy by down-regulating negative growth factors, FoxO1, MuRF1 and Atrogin-1. The results were also supported by puromycin labelling and protein ubiquitination assays. These data revealed that HA-19 possessed a "dual effect" on inhibition of muscle atrophy. In disuse-induced muscle atrophy mice model, HA-19 treatment significantly increased the weights of bilateral tibialis anterior (TA), gastrocnemius (Gastroc.), quadriceps (Quad.), suggesting the effectiveness of HA-19 to remit disuse-induced muscle atrophy. Our finding demonstrated that HA-19 has a great potential as an inhibitor or lead compound for the anti-muscle atrophy drug discovery.

6.
J Orthop Surg Res ; 14(1): 77, 2019 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-30866978

RESUMO

OBJECTIVES: There is an increased interest in enhanced recovery after surgery (ERAS) minimizing adverse events after orthopedic surgery. Little consensus supports the effectiveness of these interventions. The purpose of present systematic review and meta-analysis is to comprehensively analyze and evaluate the significance of ERAS interventions for postoperative outcomes after orthopedic surgery. METHODS: PubMed, EMBASE, and Cochrane databases were totally searched from the inception dates to May 31, 2018. Two reviewers independently extracted the data from the selected articles using a standardized form and assessed the risk of bias. The analysis was performed using STATA 12.0. RESULTS: A total of 15 published studies fulfilled the requirements of inclusion criteria. We found that the ERAS group showed a significant association with lower incidence of postoperative complications (OR, 0.70; 95% CI, 0.64 to 0.78). Meanwhile, ERAS was also associated with the decline in 30-day mortality rate and Oswestry Disability Index (ODI). However, no significant differences were identified between the two groups regarding the 30-day readmission rate (P = 0.397). CONCLUSIONS: Our meta-analysis suggested that the ERAS group had more advantages in reducing incidence of postoperative complications, 30-day mortality rate, and ODI after orthopedic surgery, but not of 30-day readmission rate. However, further research with standardized, unbiased methods and larger sample sizes is required for deeper analysis.


Assuntos
Tempo de Internação/tendências , Procedimentos Ortopédicos/tendências , Readmissão do Paciente/tendências , Cuidados Pós-Operatórios/tendências , Recuperação de Função Fisiológica/fisiologia , Idoso , Idoso de 80 Anos ou mais , Humanos , Pessoa de Meia-Idade , Procedimentos Ortopédicos/efeitos adversos , Procedimentos Ortopédicos/métodos , Cuidados Pós-Operatórios/métodos
7.
BMJ Open ; 9(1): e024110, 2019 01 28.
Artigo em Inglês | MEDLINE | ID: mdl-30696677

RESUMO

INTRODUCTION: The optimal treatment for burst fractures of the thoracolumbar spine is controversial. The addition of screws in the fractured segment has been shown to improve construct stiffness, but can aggravate the trauma to the fractured vertebra. Therefore, optimised placement of two pedicle screws at the fracture level is required for the treatment of thoracolumbar burst fractures. This randomised controlled study is the first to examine the efficacy of diverse orders of pedicle screw placement and will provide recommendations for the treatment of patients with thoracolumbar burst fractures. METHODS AND ANALYSIS: A randomised controlled trial with blinding of patients and the statistician, but not the clinicians and researchers, will be conducted. A total of 70 patients with single AO type A3 or A4 thoracolumbar fractures who are candidates for application of short-segment pedicle screws at the fractured vertebral level will be allocated randomly to the distraction-screw and screw-distraction groups at a ratio of 1:1. The primary clinical outcome measures will be the percentage loss of vertebral body height, screw depth in the injured vertebrae and kyphosis (Cobb angle). Secondary clinical outcome measures will be complications, Visual Analogue Scale scores for back and leg pain, neurological function, operation time, intraoperative blood loss, Japanese Orthopaedic Association score and Oswestry Disability Index. These parameters will be evaluated preoperatively, intraoperatively, on postoperative day 3, and at 1, 3, 6, 12 and 24 months postoperatively. ETHICS AND DISSEMINATION: The Institutional Review Board of the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University have reviewed and approved this study (batch: LCKY2018-05). The results will be presented in peer-reviewed journals and at an international spine-related meeting after completion of the study. TRIAL REGISTRATION NUMBER: NCT03384368; Pre-results.

8.
J Cell Physiol ; 234(5): 6940-6950, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30417459

RESUMO

Osteoarthritis (OA) is characterized as the degeneration and destruction of articular cartilage. In recent decades, leonurine (LN), the main active component in medical and edible dual purpose plant Herba Leonuri, has been shown associated with potent anti-inflammatory effects in several diseases. In the current study, we examined the protective effects of LN in the inhibition of OA development as well as its underlying mechanism both in vitro and in vivo experiments. In vitro, interleukin-1 beta (IL-1ß) induced over-production of prostaglandin E2, nitric oxide, inducible nitric oxide synthase, cyclooxygenase-2, interleukin-6 and tumor necrosis factor alpha were all inhibited significantly by the pretreatment of LN at a dose-dependent manner (5, 10, and 20 µM). Moreover, the expression of thrombospondin motifs 5 (ADAMTS5) and metalloproteinase 13 (MMP13) was downregulated by LN. All these changes led to the IL-1ß induced degradation of extracellular matrix. Mechanistically, the LN suppressed IL-1ß induced activation of the PI3K/Akt/NF-κB signaling pathway cascades. Meanwhile, it was also demonstrated in our molecular docking studies that LN had strong binding abilities to PI3K. In addition, LN was observed exerting protective effects in a surgical induced model of OA. To sum up, this study indicated LN could be applied as a promising therapeutic agent in the treatment of OA.

9.
J Spine Surg ; 4(3): 645-653, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30547131

RESUMO

Rapid development of 3D printing techniques has led to the design of navigation templates to assist with accurate insertion of pedicle screws in last decades. However, there are still without the precise step-by-step methods to design 3D navigation templates from computed tomography (CT) images. Our present article provides a detailed protocol to allow the readers or researchers to obtain the 3D navigation template easily, and assist with pedicle screw insertion in their future research and surgery. Using 3D navigation template-assisted pedicle screw fixation in spine surgery is low cost and can decrease the radiation exposure to both patients and surgeons.

10.
Food Funct ; 9(9): 4865-4875, 2018 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-30160278

RESUMO

Osteoarthritis (OA) is characterized by the degeneration and destruction of articular cartilage. Allicin, a dietary garlic active constituent, exerts anti-inflammatory effects on several diseases. However, its effects on OA have not been clearly elucidated. In this study, we explored the effects of allicin on OA in both in vitro and in vivo models. Allicin inhibited interleukin-1ß (IL-1ß) induced overproduction of nitric oxide, inducible nitric oxide synthase, prostaglandin E2, and cyclooxygenase-2, as well as pro-inflammatory cytokines tumor necrosis factor alpha and interleukin-6 in chondrocytes in a dose-dependent manner. Meanwhile, allicin reversed the overproduction of metalloproteinase-13 and a disintegrin and metalloproteinase with thrombospondin motifs-5 and the decrease of aggrecan and type II collagen. Furthermore, allicin dramatically suppressed IL-1ß-stimulated PI3K/Akt/NF-κB activation in chondrocytes. In vivo, treatment with allicin prevented the destruction of cartilage and inhibited PI3K/Akt/NF-κB activation in the cartilage of mice OA models. Taken together, these results indicate that allicin may be a potential therapeutic agent for OA.


Assuntos
Conservadores da Densidade Óssea/uso terapêutico , Condrócitos/metabolismo , Suplementos Nutricionais , Modelos Animais de Doenças , Osteoartrite do Joelho/terapia , Ácidos Sulfínicos/uso terapêutico , Animais , Conservadores da Densidade Óssea/efeitos adversos , Conservadores da Densidade Óssea/metabolismo , Sobrevivência Celular , Células Cultivadas , Condrócitos/citologia , Condrócitos/patologia , Suplementos Nutricionais/efeitos adversos , Progressão da Doença , Feminino , Regulação da Expressão Gênica , Humanos , Interleucina-1beta/antagonistas & inibidores , Interleucina-1beta/genética , Interleucina-1beta/metabolismo , Masculino , Camundongos Endogâmicos C57BL , NF-kappa B/agonistas , NF-kappa B/antagonistas & inibidores , NF-kappa B/metabolismo , Osteoartrite do Joelho/metabolismo , Osteoartrite do Joelho/patologia , Osteoartrite do Joelho/fisiopatologia , Fosfatidilinositol 3-Quinase/química , Fosfatidilinositol 3-Quinase/metabolismo , Proteínas Proto-Oncogênicas c-akt/agonistas , Proteínas Proto-Oncogênicas c-akt/antagonistas & inibidores , Proteínas Proto-Oncogênicas c-akt/metabolismo , Distribuição Aleatória , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Transdução de Sinais , Ácidos Sulfínicos/efeitos adversos , Ácidos Sulfínicos/metabolismo
11.
Food Funct ; 9(8): 4443-4456, 2018 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-30070297

RESUMO

The inflammatory environment has been demonstrated to be strongly associated with the progression of osteoarthritis (OA). HSYA, the main active component in the medical and edible dual purpose plant safflower, has previously showed significant anti-inflammatory effects in several diseases. In the current study, the protective effects of HSYA in the inhibition of OA development and its underlying mechanism were examined by both in vitro and in vivo experiments. Our data indicated that interleukin-1 beta (IL-1ß) induced over-production of pro-inflammatory cytokines, such as nitric oxide (NO) and prostaglandin E2 (PGE2); also, the expression of cyclooxygenase-2 (COX-2), tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6) and inducible nitric oxide synthase (iNOS) were all inhibited by pretreatment with HSYA in a dose-dependent manner (2.5 to 40 µM). Furthermore, HSYA attenuated IL-1ß-induced degradation of the extracellular matrix (ECM) by decreasing the expression of metalloproteinases (MMPs) and thrombospondin motifs 5 (ADAMTS5). Mechanistically, HSYA suppressed IL-1ß-induced activation of the nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) cascades. Meanwhile, molecular docking studies revealed that HSYA has excellent binding abilities to p65, extracellular signal-regulated kinase (ERK), p38 and c-Jun N-terminal kinase (JNK). In addition, the protective effects of HSYA were observed in a surgically induced mouse OA model. In summary, this study provides evidence that HSYA can be applied as a potential therapeutic agent in the treatment of OA.


Assuntos
Chalcona/análogos & derivados , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , NF-kappa B/metabolismo , Osteoartrite/prevenção & controle , Quinonas/administração & dosagem , Proteína ADAMTS5/genética , Proteína ADAMTS5/metabolismo , Animais , Chalcona/administração & dosagem , Ciclo-Oxigenase 2/genética , Ciclo-Oxigenase 2/metabolismo , Dinoprostona/metabolismo , Humanos , Interleucina-1beta/genética , Interleucina-1beta/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , NF-kappa B/genética , Óxido Nítrico/metabolismo , Óxido Nítrico Sintase Tipo II/genética , Óxido Nítrico Sintase Tipo II/metabolismo , Osteoartrite/genética , Osteoartrite/metabolismo , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/metabolismo
12.
Ann Transl Med ; 6(6): 105, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29707554

RESUMO

Background: Compare the efficacy and safety of minimally invasive and open transforaminal lumbar interbody fusion (TLIF) in the treatment of single segmental lumbar spondylolisthesis. Methods: From 2010-01 to 2015-10, in total, 167 patients with single segmental spondylolisthesis treated by TLIF were included, 79 cases in minimally invasive TLIF (MI-TLIF) group and 88 cases in open TLIF group. The peri-operative parameters of operative time, estimated blood loss and length of postoperative hospital stay was recorded, as well as complications. Visual Analogue Scale (VAS) of low back pain and leg pain, and Oswestry Disability Index (ODI) were used to assess the pain and functional outcomes at pre-operatively, 3 months/1 year/2 years/5 years after operation. The radiographic parameters of posterior height of the intervertebral space and segmental lordosis were measured too. Results: No significantly difference was found at baseline characteristic data of age, gender ratio, the percentage of degenerative and isthmic spondylolisthesis, the percentage of slip, and segmental distribution between MI-TLIF and open TLIF groups. MI-TLIF group had less estimated intra-operative blood loss (163.7±49.6 mL) than open TLIF group (243.3±70.2 mL, P<0.001) and had shorter post-operative hospital stay (5.8±1.4 days) than open TLIF group (7.3±2.9 days, P<0.001). Both MI-TLIF and open TLIF can significantly reduce the VAS of low back pain, VAS of leg pain, ODI, and improve the posterior height of the intervertebral space and segmental lordosis, but no significantly difference was found of them between two groups. Conclusions: Our study suggests that MI-TLIF is a safe and effective choice in the treatment of lower grade lumbar spondylolisthesis (grade II or less), and it has advantages of less blood loss, postoperative hospital stay when compared to open TLIF.

13.
J Bone Miner Metab ; 36(6): 691-699, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29280077

RESUMO

The objective of this study was to investigate the effects of human parathyroid hormone (1-34) (PTH1-34; PTH) plus menaquinone-4 (vitamin K2; MK) on the osseous integration of hydroxyapatite (HA)-coated implants in osteoporotic rats. Ovariectomized female Sprague-Dawley rats were used for the study. Twelve weeks after bilateral ovariectomy, HA-coated titanium implants were inserted bilaterally in the femoral medullary canal of the remaining 40 ovariectomized rats. All animals were then randomly assigned to four groups: Control, MK, PTH and PTH + MK. The rats from groups MK, PTH and PTH + MK received vitamin K2 (30 mg/kg/day), PTH1-34 (60 µg/kg, three times a week), or both for 12 weeks. Thereafter, serum levels of γ-carboxylated osteocalcin (Gla-OC) were quantitated by ELISA and the bilateral femurs of rats were harvested for evaluation. The combination of PTH and MK clearly increased the serum levels of Gla-OC (a specific marker for bone formation) compared to PTH or MK alone. The results of our study indicated that all treated groups had increased new bone formation around the surface of implants and increased push-out force compared to Control. In addition, PTH + MK treatment showed the strongest effects in histological, micro-computed tomography and biomechanical tests. In summary, our results confirm that treatment with PTH1-34 and MK together may have a therapeutic advantage over PTH or MK monotherapy on bone healing around HA-coated implants in osteoporotic rats.


Assuntos
Materiais Revestidos Biocompatíveis/química , Durapatita/química , Fêmur/patologia , Osteoporose/tratamento farmacológico , Hormônio Paratireóideo/uso terapêutico , Próteses e Implantes , Titânio/química , Vitamina K 2/análogos & derivados , Animais , Biomarcadores/metabolismo , Fenômenos Biomecânicos , Feminino , Osteoporose/diagnóstico por imagem , Osteoporose/patologia , Osteoporose/fisiopatologia , Hormônio Paratireóideo/farmacologia , Implantação de Prótese , Ratos Sprague-Dawley , Vitamina K 2/farmacologia , Vitamina K 2/uso terapêutico , Microtomografia por Raio-X
14.
J Sports Med Phys Fitness ; 58(7-8): 1090-1095, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-28229565

RESUMO

Calcifying tendinitis of the shoulder is characterized with the formation of the calcium deposits in tendon, which mostly involves supraspinatus. The formation of the calcium deposits may be related to the excessive mechanical stimulation and non-tenocytes generation from tendon stem cell. The disease is usually associated with pain, especially when activating the shoulder. We describe one case of calcifying tendinitis involving the long head of the biceps brachii and superior labrum. The related literature and researches are also reviewed. After reviewing the past articles, there are no similar cases of calcification involving the long head of the biceps brachii and superior labrum. The diagnosis and treatment scheme may be similar with Calcifying tendinitis of the supraspinatus. The differential diagnosis of calcification involving the long head of the biceps brachii and superior labrum and glenoid cavity fracture is very important. Calcifying tendinitis involving the long head of the biceps brachii and superior labrum is rare and the diagnosis need to rule out fractures. The surgical treatment of arthroscopy is effective and relieves the symptoms quickly, but may not be the first choice.


Assuntos
Calcinose/patologia , Articulação do Ombro/lesões , Tendinopatia/patologia , Artroscopia , Calcinose/complicações , Calcinose/cirurgia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Manguito Rotador/diagnóstico por imagem , Articulação do Ombro/diagnóstico por imagem , Articulação do Ombro/cirurgia , Tendinopatia/complicações , Tendinopatia/cirurgia , Tomografia Computadorizada por Raios X
15.
Mol Med Rep ; 17(3): 3751-3757, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29286130

RESUMO

Dexamethasone (Dex) and other glucocorticoids are widely used to treat serious infections and immunological diseases, however they may cause steroid­induced avascular necrosis of the femoral head (SANFH). Salidroside (Sal) has demonstrated an anti­apoptotic effect on neurocytes by activating the phosphoinositide 3­kinase (PI3K)/protein kinase B (Akt) signaling pathway. In the present study, primary osteoblasts were used in vitro and in rats in vivo to determine the anti-apoptotic effect of Sal on SANFH. The result of the present study demonstrated that pretreatment with Sal increased the cell survival rate while decreasing the cell apoptosis and lactate dehydrogenase release rate. Additionally, Sal also caused the reduction of TUNEL positive cells in TUNEL staining assay. Sal decreased the expression of cleaved caspase-3, cleaved caspase­9, apoptosis regulator BAX and cytochrome C, while it increased the expression of B cell lymphoma­2 and phosphorylated­Akt in Dex­induced osteoblasts. In vivo Sal protected against SANFH in rats by decreasing the percentage of empty lacunae. The present study demonstrated that Sal alleviated Dex­induced osteoblast apoptosis by activating the PI3K/Akt signaling pathway and downregulating caspase­3 expression in osteoblasts. Sal also protected against SANFH in a rat model of SANFH by decreasing the percentage of empty lacunae. The inhibition of the mitochondrial apoptosis pathway was also involved. Further research is required to determine the full underlying mechanisms by which Sal has an effect.


Assuntos
Glucosídeos/farmacologia , Fenóis/farmacologia , Transdução de Sinais/efeitos dos fármacos , Animais , Apoptose/efeitos dos fármacos , Caspase 3/metabolismo , Caspase 9/metabolismo , Sobrevivência Celular/efeitos dos fármacos , Citocromos c/metabolismo , Dexametasona/toxicidade , Necrose da Cabeça do Fêmur/induzido quimicamente , Necrose da Cabeça do Fêmur/patologia , Necrose da Cabeça do Fêmur/veterinária , Masculino , Camundongos , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Osteoblastos/citologia , Osteoblastos/efeitos dos fármacos , Osteoblastos/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Ratos , Ratos Sprague-Dawley , Proteína X Associada a bcl-2/metabolismo
16.
Biomed Res Int ; 2017: 8423638, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28154826

RESUMO

Purpose. To investigate the evidence of minimally invasive (MI) versus open (OP) posterior lumbar fusion in treatment of lumbar spondylolisthesis from current prospective literatures. Methods. The electronic literature database of Pubmed, Embase, and Cochrane library was searched at April 2016. The data of operative time, estimated blood loss and length of hospital stay, visual analog scale (VAS) of both lower back pain and leg pain, Oswestry disability index (ODI), SF-36 PCS (physical component scores) and SF-36 MCS (mental component scores), complications, fusion rate, and secondary surgery were extracted and analyzed by STATA 12.0 software. Results. Five nonrandom prospective comparative studies were included in this meta-analysis. The meta-analysis showed that the MI group had a significantly longer operative time than OP group, less blood loss, and shorter hospital stay. No significant difference was found in back pain, leg pain, ODI, SF-36 PCS, SF-36 MCS, complications, fusion rate, and secondary surgery between MI and OP groups. Conclusion. The prospective evidence suggested that MI posterior fusion for spondylolisthesis had less EBL and hospital stay than OP fusion; however it took more operative time. Both MI and OP fusion had similar results in pain and functional outcomes, complication, fusion rate, and secondary surgery.


Assuntos
Vértebras Lombares/cirurgia , Região Lombossacral/cirurgia , Espondilolistese/cirurgia , Adulto , Idoso , Dor nas Costas/cirurgia , Perda Sanguínea Cirúrgica , Feminino , Humanos , Tempo de Internação , Masculino , Pessoa de Meia-Idade , Procedimentos Cirúrgicos Minimamente Invasivos/métodos , Duração da Cirurgia , Medição da Dor/métodos , Estudos Prospectivos , Fusão Vertebral/métodos , Resultado do Tratamento , Escala Visual Analógica
17.
Eur Spine J ; 26(7): 1878-1883, 2017 07.
Artigo em Inglês | MEDLINE | ID: mdl-28102450

RESUMO

PURPOSE: Percutaneous kyphoplasty (PKP) is a minimally invasive procedure for the treatment of osteoporotic vertebral compression fractures (OVCFs). It is generally considered that there is little blood loss during the surgery. However, a significant perioperative hidden blood loss (HBL) is neglected. This study was to examine the amount of HBL and determine the influential factors during PKP. METHODS: From January 2015 to January 2016, 115 patients with OVCFs who were scheduled to have a PKP were enrolled in this study. The factors analyzed included gender, age, body mass index (BMI), percentage of vertebral height loss, percentage of vertebral height restoration, number of fracture levels, bone mineral density (BMD), duration of symptom, cement leakage, and other internal diseases (hypertension, diabetes mellitus). According to Gross's formula, each patient's height, weight, and pre-operative and post-operative hematocrit were recorded and used for calculating the blood loss. Influential factors were further analyzed by multivariate linear regression analysis and t test. RESULTS: The mean HBL was 282 ± 162 mL (mL) and the post-operative Hb loss was 8.7 ± 5.4 g per liter (g/L). According to multivariate linear regression analysis, patients with severe vertebral height loss (P = 0.016), better vertebral height restoration (P = 0.038), and multi-segmental vertebral fractures (P = 0.000) had a higher amount of HBL. Fresh fractures (P = 0.008) and cement leakage (P = 0.004) were also important factors to increase HBL, whereas gender (P = 0.642), age (P = 0.203), BMI (P = 0.075), hypertension (P = 0.099), diabetes mellitus (P = 0.905), and BMD (P = 0.521) were not correlate with HBL. When we compared the incidence of anemia between pre-operative and post-operative, we found that the incidence of anemia was significantly associated with HBL (P = 0.000). CONCLUSIONS: HBL cannot be ignored in perioperative period, especially for poor physical condition and multiple fractures patients. Having a correct understanding of HBL can help improve clinical assessment capabilities, ensuring patients' safety.


Assuntos
Perda Sanguínea Cirúrgica/estatística & dados numéricos , Fraturas por Compressão/cirurgia , Cifoplastia/efeitos adversos , Procedimentos Cirúrgicos Minimamente Invasivos/efeitos adversos , Fraturas por Osteoporose/cirurgia , Hemorragia Pós-Operatória/etiologia , Fraturas da Coluna Vertebral/cirurgia , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Cifoplastia/métodos , Modelos Lineares , Masculino , Pessoa de Meia-Idade , Hemorragia Pós-Operatória/diagnóstico , Hemorragia Pós-Operatória/epidemiologia , Estudos Retrospectivos , Fatores de Risco , Resultado do Tratamento
18.
IEEE Trans Cybern ; 47(1): 142-152, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26841429

RESUMO

This paper presents a half-face dictionary integration (HFDI) algorithm for representation-based classification. The proposed HFDI algorithm measures residuals between an input signal and the reconstructed one, using both the original and the synthesized dual-column (row) half-face training samples. More specifically, we first generate a set of virtual half-face samples for the purpose of training data augmentation. The aim is to obtain high-fidelity collaborative representation of a test sample. In this half-face integrated dictionary, each original training vector is replaced by an integrated dual-column (row) half-face matrix. Second, to reduce the redundancy between the original dictionary and the extended half-face dictionary, we propose an elimination strategy to gain the most robust training atoms. The last contribution of the proposed HFDI method is the use of a competitive fusion method weighting the reconstruction residuals from different dictionaries for robust face classification. Experimental results obtained from the Facial Recognition Technology, Aleix and Robert, Georgia Tech, ORL, and Carnegie Mellon University-pose, illumination and expression data sets demonstrate the effectiveness of the proposed method, especially in the case of the small sample size problem.

19.
IEEE Trans Image Process ; 24(11): 3425-40, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26087493

RESUMO

A large amount of training data is usually crucial for successful supervised learning. However, the task of providing training samples is often time-consuming, involving a considerable amount of tedious manual work. In addition, the amount of training data available is often limited. As an alternative, in this paper, we discuss how best to augment the available data for the application of automatic facial landmark detection. We propose the use of a 3D morphable face model to generate synthesized faces for a regression-based detector training. Benefiting from the large synthetic training data, the learned detector is shown to exhibit a better capability to detect the landmarks of a face with pose variations. Furthermore, the synthesized training data set provides accurate and consistent landmarks automatically as compared to the landmarks annotated manually, especially for occluded facial parts. The synthetic data and real data are from different domains; hence the detector trained using only synthesized faces does not generalize well to real faces. To deal with this problem, we propose a cascaded collaborative regression algorithm, which generates a cascaded shape updater that has the ability to overcome the difficulties caused by pose variations, as well as achieving better accuracy when applied to real faces. The training is based on a mix of synthetic and real image data with the mixing controlled by a dynamic mixture weighting schedule. Initially, the training uses heavily the synthetic data, as this can model the gross variations between the various poses. As the training proceeds, progressively more of the natural images are incorporated, as these can model finer detail. To improve the performance of the proposed algorithm further, we designed a dynamic multi-scale local feature extraction method, which captures more informative local features for detector training. An extensive evaluation on both controlled and uncontrolled face data sets demonstrates the merit of the proposed algorithm.


Assuntos
Identificação Biométrica/métodos , Face/anatomia & histologia , Imagem Tridimensional/métodos , Algoritmos , Simulação por Computador , Bases de Dados Factuais , Humanos
20.
Planta ; 237(5): 1367-78, 2013 May.
Artigo em Inglês | MEDLINE | ID: mdl-23397191

RESUMO

The ASYMMETRIC LEAVES2-LIKE/LATERAL ORGAN BOUNDARIES (LOB) DOMAIN (ASL/LBD) genes encode plant-specific nuclear proteins containing the conserved domain AS2/LOB. In this study, the function of a member of the Arabidopsis thaliana AS2/LOB gene family, ASL11/LBD15, was investigated. The results show that ASL11/LBD15 is expressed in the meristems of shoot apex, root apex, organ boundaries, and developing seeds. Overexpression of ASL11/LBD15 resulted in aberrant arrangements in the tunica cell layers of the shoot apical meristem (SAM). Two-week-old transgenic plants developed needle-like leaves in addition to regular leaves, while 6-week-old transformants displayed clustered cauline leaves suggesting altered SAM development. qRT-PCR analysis revealed that the WUSCHEL (WUS) transcript level was strongly up-regulated in plants overexpressing ASL11/LBD15 compared with the wild-type plants. Furthermore, inducible ASL11/LBD15 ectopic expression activated ectopic expression of WUS and affected the differentiation of leaf epidermal cells. Therefore, our results suggest that ASL11/LBD15 affects cellular differentiation in the SAM and regulates WUS expression.


Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Proteínas de Homeodomínio/metabolismo , Meristema/metabolismo , Arabidopsis/genética , Arabidopsis/crescimento & desenvolvimento , Proteínas de Arabidopsis/genética , Regulação da Expressão Gênica de Plantas/genética , Regulação da Expressão Gênica de Plantas/fisiologia , Proteínas de Homeodomínio/genética , Meristema/genética , Meristema/crescimento & desenvolvimento , Folhas de Planta/genética , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Brotos de Planta/genética , Brotos de Planta/crescimento & desenvolvimento , Brotos de Planta/metabolismo
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