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1.
Artigo em Inglês | MEDLINE | ID: mdl-33573897

RESUMO

INTRODUCTION: The effects of Invisalign clear aligner treatment with and without Dental Monitoring (DM) were compared for treatment duration, number of appointments, refinements and refinement aligners, and accuracy of Invisalign in achieving predicted tooth positions (aligner tracking). The null hypothesis was that there are no differences between Invisalign with and without DM in these parameters. METHODS: A sample of 90 consecutively treated Invisalign patients (45 control, 45 DM) fitted the inclusion and exclusion criteria. Treatment duration, number of refinements, number of refinement aligners, time to first refinement, number of appointments, number of emergency visits, and accuracy of predicted tooth movement were observed for differences. RESULTS: The 2 groups were homogeneous (P >0.05) for sample size, age, gender, Angle classification, maxillary and mandibular irregularity index, and the number of initial aligners. There was a significant (P = 0.001) reduction in the number of appointments by 3.5 visits (33.1%) in the DM group. There was also a significant (P = 0.001) reduction in the time to the first refinement (1.7 months) in the DM group. Compared with Invisalign predicted tooth positions, actual tooth positions were statistically (P <0.05) more accurate for the DM group for the maxillary anterior dentition in rotational movements and mandibular anterior dentition for buccal-lingual linear movement. Invisalign therapy without DM was closer to predicted tooth positions for the maxillary posterior dentition for the tip. None of these differences surpassed the clinically significant thresholds (>0.5 mm or >2°); however, the DM group achieved this in 1.7 fewer months. CONCLUSIONS: DM with Invisalign therapy resulted in a reduced number of appointments by 3.5 visits (33.1%). The DM group also achieved a clinically similar accuracy in obtaining predicted tooth movements compared with the control group in 1.7 fewer months, indicating improved aligner tracking in the DM group.

2.
Prog Orthod ; 22(1): 3, 2021 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-33458787

RESUMO

BACKGROUND: Patient quality of life (QoL) during orthodontic treatment is an important consideration that requires greater academic investigation as greater focus is placed on enhancing patient experience. Quality of life (QoL) was assessed in three orthodontic appliance groups, i.e., vestibular, lingual, and aligners during the initial stages of treatment. The sample was comprised of 117 adult patient-subjects distributed into 3 groups: vestibular (n = 41), lingual (n = 37), and aligner (n = 39). A WHOQOL-BREF questionnaire surveyed four domains (physical health, psychological health, social relationships, and environment). RESULTS: Mean scores for domain 1, physical health, showed that the aligner group (28.1) had significantly greater scores than that of the vestibular (22.7) or lingual (22) groups. Domain 2, psychological health, demonstrated significant differences (P < 0.001) between all groups, with the aligner group scoring the highest (23.2), followed by the lingual (18.4) and vestibular (15.2) groups. Domain 3, social relationship, showed that aligner (10.9) and lingual (10.2) scores were significantly greater (P < 0.001) than those of the vestibular group (7.8). Domain 4, environment, displayed significant differences between all groups, with the aligner group scoring highest (32.1), followed by the lingual group (29.3), and lastly the vestibular group (26.4). Overall, the highest mean score was obtained by the aligner group (23.1) and the lowest mean score was by the vestibular group (18). The mean domain scores for all three groups were significantly different (P ≤ 0.005) from each other (Table 2). CONCLUSIONS: Overall, patients undergoing Aligner therapy reported the overall highest QoL scores, followed by lingual and vestibular groups.


Assuntos
Aparelhos Ortodônticos , Qualidade de Vida , Adulto , Humanos , Desenho de Aparelho Ortodôntico , Inquéritos e Questionários
3.
Angle Orthod ; 2020 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-33296455

RESUMO

OBJECTIVES: To compare the efficacy of orthodontic tooth movement with three aligner wear protocols: 7 day, 10 day, and 14 day. MATERIALS AND METHODS: Eighty patients were randomly allocated into three groups: group A (7-day changes), group B (10-day changes), and group C (14-day changes). The posttreatment scans were compared with the final virtual treatment simulations through digital superimposition. The differences between predicted and actual achieved treatment outcomes were computed in six angular and six linear dimensions. Differences >0.5 mm for linear measurements and >2° for angular measurements were considered clinically relevant. RESULTS: Within groups, and irrespective of wear protocol, all linear discrepancies in both jaws were deemed clinically insignificant (<0.5 mm) while nearly all angular discrepancies were considered clinically significant (>2.0°). When the three groups were compared, group C (14-day changes) showed significantly greater accuracy in the posterior segment for maxillary intrusion, distal-crown tip and buccal-crown torque, and mandibular intrusion and extrusion. The mean treatment duration in the 7-day aligner change group was nearly half that of the 14-day aligner change group (5 months vs 9 months). CONCLUSIONS: Fourteen-day changes were statistically significantly more accurate in some posterior movements. However, this difference in accuracy did not exceed the threshold for clinical significance (>0.5 mm/>2.0°). Achieving a clinically similar accuracy between the 7-day protocol and 14-day protocol in half the treatment time suggests a 7-day protocol as an acceptable treatment protocol. Clinicians may consider slowing down to a 14-day protocol if challenging posterior movements are desired.

4.
Am J Orthod Dentofacial Orthop ; 158(3): 420-425, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32620479

RESUMO

INTRODUCTION: The purpose of this research was to provide an update on the accuracy of tooth movement with Invisalign (Align Technology, Santa Clara, Calif). METHODS: This prospective clinical study included 38 patients treated with Invisalign Full or Invisalign Teen. All teeth, from the central incisor to the second molar, were measured on digital models created from intraoral scans. Predicted values were determined by superimposing the initial and final ClinCheck models, and achieved values were determined by superimposing the initial ClinCheck models and the digital models from the posttreatment scans. Individual teeth were superimposed with a best-fit analysis and measured using Compare software (version 8.1; GeoDigm, Falcon Heights, Minn). The types of tooth movements studied were a mesial-distal crown tip, buccal-lingual crown tip, extrusion, intrusion, and mesial-distal rotation. RESULTS: The mean accuracy of Invisalign for all tooth movements was 50%. The highest overall accuracy was achieved with a buccal-lingual crown tip (56%), whereas the lowest overall accuracy occurred with rotation (46%). The accuracies for mesial rotation of the mandibular first molar (28%), distal rotation of the maxillary canine (37%), and intrusion of the mandibular incisors (35%) were particularly low. CONCLUSIONS: There was a marked improvement in the overall accuracy; however, the strengths and weaknesses of tooth movement with Invisalign remained relatively the same.


Assuntos
Desenho de Aparelho Ortodôntico , Aparelhos Ortodônticos Removíveis , Adolescente , Seguimentos , Humanos , Estudos Prospectivos , Técnicas de Movimentação Dentária
5.
Front Microbiol ; 10: 2572, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31787957

RESUMO

Recent studies indicate that environmentally abundant quaternary amines (QAs) are a primary source for methanogenesis, yet the catabolic enzymes are unknown. We hypothesized that the methanogenic archaeon Methanolobus vulcani B1d metabolizes glycine betaine (GB) through a corrinoid-dependent GB:coenzyme M (CoM) methyl transfer pathway. The draft genome sequence of M. vulcani B1d revealed a gene encoding a predicted non-pyrrolysine MttB homolog (MV8460) with high sequence similarity to the GB methyltransferase encoded by Desulfitobacterium hafniense Y51. MV8460 catalyzes GB-dependent methylation of free cob(I)alamin indicating it is an authentic MtgB enzyme. Proteomic analysis revealed that MV8460 and a corrinoid binding protein (MV8465) were highly abundant when M. vulcani B1d was grown on GB relative to growth on trimethylamine. The abundance of a corrinoid reductive activation enzyme (MV10335) and a methylcorrinoid:CoM methyltransferase (MV10360) were significantly higher in GB-grown B1d lysates compared to other homologs. The GB:CoM pathway was fully reconstituted in vitro using recombinant MV8460, MV8465, MV10335, and MV10360. Demonstration of the complete GB:CoM pathway expands the knowledge of direct QA-dependent methylotrophy and establishes a model to identify additional ecologically relevant anaerobic quaternary amine pathways.

6.
Int J Periodontics Restorative Dent ; 39(5): e188-e194, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31449579

RESUMO

This case report describes a patient with severe (11-mm) mandibular anterior crowding who received periodontally accelerated osteogenic orthodontics (PAOO) nonextraction treatment, showing 5-year follow-up. A 15-year-old female presented with severe dental crowding, Angle Class I skeletal and molar relationships and a Class II canine relationship, and excessive overjet and overbite. Following fixed-appliance placement (0.022-inch, MBT prescription), full-thickness periosteal flaps beyond the tooth apices were raised bilaterally from the second molars in both arches, and selective decortication of cortical bone was performed on the facial and lingual sides using a surgical tool. Bone grafting material, comprised of a mixture of demineralized freeze-dried bone allograft (DFDBA) and bovine bone (Bio Oss, Geistlich), was placed at the corticotomy sites. The patient was seen every 2 weeks after the surgery for orthodontic adjustments; the total active orthodontic treatment time was 9 months. Limits of tooth movement (the scope of treatment) were increased by the bone-graft augmentation. At the 5-year follow-up, cephalometric comparisons to baseline conditions showed dramatic incisor changes and stable dentoalveolar effects. This case demonstrates the efficacy of PAOO as a useful adjunct orthodontic strategy for adult patients who require treatment of severe crowding, ie, treatment that would be considered unreasonable if conventional orthodontics were employed. When conventional orthodontics cannot achieve the treatment goal and orthognathic surgery is not feasible, PAOO expands the scope of conventional orthodontic treatment in the adult 2-fold to 3-fold in most spatial dimensions.


Assuntos
Má Oclusão de Angle Classe II , Má Oclusão , Ortodontia , Adolescente , Adulto , Animais , Bovinos , Cefalometria , Feminino , Humanos , Mandíbula , Técnicas de Movimentação Dentária
7.
Angle Orthod ; 89(5): 697-704, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-30888841

RESUMO

OBJECTIVES: To compare forced-eruption times for palatally impacted canines treated with and without the ostectomy-decortication technique and to assess the influence of palatally impacted canine pretreatment position and angle on forced-eruption time. MATERIALS AND METHODS: The sample was composed of 118 patient-subjects with 151 palatally impacted canines treated with the ostectomy-decortication technique (n = 72) and without (n = 79). The orthopantomogram radiographs (OPGs) were analyzed for palatally impacted canine angle and horizontal and vertical position. Recovery time was measured from the start of forced eruption until the canine was within ±1 mm of final dental arch position. RESULTS: The time of forced canine eruption with ostectomy-decortication technique was significantly shorter than without (6.6 vs 21.0 months). Pretreatment canine position significantly increased forced-eruption time in the ostectomy-decortication group but not in the control sample. CONCLUSIONS: Forced-eruption time of palatally impacted canines using the ostectomy-decortication technique was 3.2 times more rapid than without. Forced-eruption time increased significantly as a function of pretreatment palatally impacted canine position severity in the ostectomy-decortication group but not in the control.


Assuntos
Dente Canino , Erupção Dentária , Dente Impactado , Arco Dental , Humanos , Maxila , Extrusão Ortodôntica , Dente Impactado/terapia
8.
Eur J Dent ; 11(1): 117-121, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28435377

RESUMO

OBJECTIVE: The objective of this study is to determine the frequency of impacted maxillary canines using seven subtype classification system. For this purpose, impacted maxillary canines have been divided into seven various subtypes. MATERIALS AND METHODS: This is a descriptive, cross-sectional, and retrospective study conducted using radiographic data of residents of Madinah, Al Munawwarah. Radiographic data of 14,000 patients, who attended College of Dentistry, Taibah University, from January 2011 to February 2015, were screened against the selection criteria for the presence of impacted teeth. The individuals with maxillary impacted canines were matched to maxillary canine impaction. The occurrence of each subtype of impacted canines was calculated. RESULTS: Impacted teeth are more common in the maxilla compared to mandible. The impacted canine represented the highest proportion of all impacted maxillary teeth followed by the second premolars and the central incisors. According to the classification system represented, Type II of canine impaction comprised the highest proportion (51%) while Type IV (0.5%) comprised the lowest frequency. The maxillary canine is the most frequently impacted tooth followed by mandibular canines. CONCLUSIONS: Although there are many variations, the majority of impacted canines fall into Type II of the classification of impacted canines.

10.
J Periodontol ; 86(10): 1107-15, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26138808

RESUMO

BACKGROUND: The aim of the present study is to compare the keratinized gingival tissue (KT) height labial to the mandibular incisors after active orthodontic treatment (AOT) with and without alveolar corticotomy and bone grafting. METHODS: Two orthodontically treated groups of 35 patients each, with (Cort) and without (Conv) alveolar decortication and augmentation bone grafting, are matched in this case-control study for sample size, sex, mandibular premolar extractions, pretreatment age, post-treatment observation period, and pretreatment KT height. Standardized digital frontal occlusion photographs taken before and at least 1 year after AOT were adjusted to 96 dots per inch and measured with image analysis software for vertical KT height labial to each mandibular incisor. RESULTS: An average of 1.5 years after completion of AOT, KT height had increased significantly by 0.78 mm (P < 0.001) in the Cort group and decreased 0.38 mm (P = 0.002) in the Conv group; a 1.28-mm KT height gain was demonstrated in the subgroup representing the lowest half of Cort KT height at pretreatment. Mandibular incisor inclination and prominence explained neither the decrease in KT height in Conv nor the KT height gain in Cort. CONCLUSIONS: Orthodontic therapy combined with alveolar decortication and augmentation bone grafting resulted in a significant increase in KT height. Although KT height surrounding the dentition has been devalued by evidence-based studies, the value-added protection of KT height increase after decortication and augmentation bone grafting offsets the concerns of orthodontic proclination or expanding mandibular incisors facially.


Assuntos
Processo Alveolar/cirurgia , Aumento do Rebordo Alveolar/métodos , Transplante Ósseo/métodos , Osso Cortical/cirurgia , Gengiva/anatomia & histologia , Queratinas/análise , Adolescente , Adulto , Dente Pré-Molar/cirurgia , Estudos de Casos e Controles , Criança , Feminino , Seguimentos , Gengiva/química , Humanos , Processamento de Imagem Assistida por Computador/métodos , Incisivo/anatomia & histologia , Masculino , Mandíbula/cirurgia , Pessoa de Meia-Idade , Fotografação/métodos , Extração Dentária/métodos , Técnicas de Movimentação Dentária/métodos , Adulto Jovem
11.
Arch Microbiol ; 197(2): 197-209, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25319587

RESUMO

Two novel strains of methanogens were isolated from an estuarine sediment with the capability to utilize quaternary amines. Based on the 16S rRNA analysis, strain B1d shared 99 % sequence identity with Methanolobus vulcani PL-12/M(T) and strain Q3c shared 99 % identity with Methanococcoides sp. PM1 and PM2, but our current isolates display clearly different capabilities of growth on quaternary amines and were isolated based on these capabilities. Strain Q3c was capable of growth on tetramethylammonium and choline, while strain B1d was capable of growth on glycine betaine. Ml. vulcani PL-12/M(T) was incapable of growth on glycine betaine, indicating an obvious distinction between strains B1d and PL-12/M(T). Strain Q3c now represents the only known tetramethylammonium-utilizing methanogen in isolation. Strain B1d is the first quaternary amine-utilizing methanogen from the genus Methanolobus. This study suggests that quaternary amines may serve as ready precursors of biological methane production in marine environments.


Assuntos
Betaína/metabolismo , Methanosarcinaceae/classificação , Methanosarcinaceae/fisiologia , Filogenia , Compostos de Amônio Quaternário/metabolismo , Sedimentos Geológicos/microbiologia , Methanosarcinaceae/genética , Methanosarcinaceae/isolamento & purificação , RNA Ribossômico 16S/genética
12.
Angle Orthod ; 85(5): 743-9, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25474711

RESUMO

OBJECTIVE: To evaluate mandibular irregularity index stability following orthodontic treatment facilitated by alveolar corticotomy and augmentation bone grafting (Cort+). MATERIALS AND METHODS: The irregularity index of 121 orthodontically treated and 15 untreated patient study casts was analyzed at 5 years and 10 years. RESULTS: Cort+ resulted in significantly lower mandibular irregularity index scores at both 5 years (1.5 mm vs 4.2 mm, P < .000) and 10 years (2.1 mm vs 4.1 mm, P < .000) compared with conventionally treated patients. CONCLUSIONS: Unmatched samples advise caution with conclusions, but orthodontic therapy combined with Cort+ enhanced the stability of the postorthodontic mandibular irregularity index for at least 10 years in this preliminary study.


Assuntos
Processo Alveolar/patologia , Mandíbula/patologia , Ortodontia Corretiva/métodos , Adulto , Feminino , Humanos , Masculino , Fatores de Tempo
13.
Oral Health Dent Manag ; 13(3): 857-65, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25284571

RESUMO

AIM: The purpose of the study was to compare and contrast the orthodontic treatment needs of Dubai school-age school age children as a function of gender and ethnicity. METHODS: A total of 20,880 subjects were screened in 66 public and private school located in Dubai, United Arab Emirates. The study sample was grouped according to seven geographic regions. The total sample included 9,765 females and 11,115 males. Ages ranged from 9.08 years to 24.4 years with an overall mean age of 14.5 years. Calibrated dentists examined school age children using Peer Assessment Rating Index (PAR). PAR scores were translated to Index for Orthodontic Treatment Need (IOTN) scores based upon the description of each of the 31 IOTN line item descriptions that constitute IOTN grades from 1 to 5. Upon completion, statistical comparison of the study variables by gender and ethnicity was applied. RESULTS: Evaluation of IOTN grade by gender demonstrated significantly higher male (2.52) than female (2.47, p=0.002) grade; comparison of IOTN grade by region showed South Asia (2.58) significantly higher than Middle East (2.43). IOTN grade was highest for South Asia males (2.62) which was significantly higher than South Asia females (2.55, p=0.023) and higher than both Middle East males and females (2.45 and 2.41, p=.000). Moreover, average South Asia female IOTN grade was significantly higher (p=0.000) than for both Middle East males and females. Conclusions 1. In Dubai school-aged adolescents, 53.2% of the study sample would benefit from orthodontic treatment and 14.4% were profiled as "treatment require". 2. Within the Middle East region, UAE subjects had higher mean IOTN grade than subjects from Iran, Syria and Yemen. 3. India males had significantly higher mean IOTN grades than Middle East male and female subjects from UAE, Iran, Syria, and Yemen. 4. India females had significantly higher mean IOTN grades than Middle East female subjects from UAE, Iran, Syria, and Yemen. It may be concluded that males and females from India have the greatest orthodontic treatment need in Dubai public and private schools.

14.
Proc Natl Acad Sci U S A ; 111(43): E4668-76, 2014 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-25313086

RESUMO

COG5598 comprises a large number of proteins related to MttB, the trimethylamine:corrinoid methyltransferase. MttB has a genetically encoded pyrrolysine residue proposed essential for catalysis. MttB is the only known trimethylamine methyltransferase, yet the great majority of members of COG5598 lack pyrrolysine, leaving the activity of these proteins an open question. Here, we describe the function of one of the nonpyrrolysine members of this large protein family. Three nonpyrrolysine MttB homologs are encoded in Desulfitobacterium hafniense, a Gram-positive strict anaerobe present in both the environment and human intestine. D. hafniense was found capable of growth on glycine betaine with electron acceptors such as nitrate or fumarate, producing dimethylglycine and CO2 as products. Examination of the genome revealed genes for tetrahydrofolate-linked oxidation of a methyl group originating from a methylated corrinoid protein, but no obvious means to carry out corrinoid methylation with glycine betaine. DSY3156, encoding one of the nonpyrrolysine MttB homologs, was up-regulated during growth on glycine betaine. The recombinant DSY3156 protein converts glycine betaine and cob(I)alamin to dimethylglycine and methylcobalamin. To our knowledge, DSY3156 is the first glycine betaine:corrinoid methyltransferase described, and a designation of MtgB is proposed. In addition, DSY3157, an adjacently encoded protein, was shown to be a methylcobalamin:tetrahydrofolate methyltransferase and is designated MtgA. Homologs of MtgB are widely distributed, especially in marine bacterioplankton and nitrogen-fixing plant symbionts. They are also found in multiple members of the human microbiome, and may play a beneficial role in trimethylamine homeostasis, which in recent years has been directly tied to human cardiovascular health.


Assuntos
Betaína/metabolismo , Glicina N-Metiltransferase/metabolismo , Lisina/análogos & derivados , Metilaminas/metabolismo , Cromatografia em Camada Delgada , Desulfitobacterium/genética , Desulfitobacterium/crescimento & desenvolvimento , Genes Bacterianos , Humanos , Lisina/metabolismo , Metilação , Filogenia , Reação em Cadeia da Polimerase em Tempo Real
15.
Oral Health Dent Manag ; 13(2): 474-85, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24984668

RESUMO

AIMS: To compare and contrast oral health related data related to Simplified Oral hygiene index (OHI-S) in Dubai school-aged students as a function of the population demographics gender and ethnicity. METHODS: A total of 20,880 subjects were screened in 66 public and private school located in Dubai, United Arab Emirates. The study sample was grouped according to seven geographic regions. The total sample included 9,765 females and 11,115 males. Ages ranged from 9.08 years to 24.4 years with an overall mean age of 14.5 years. Calibrated dentists scored all subjects for Debri, Calculus and Simplified Oral hygiene index (OHI-S). Upon completion, statistical comparison of the study variables by region and gender was applied. RESULTS: Debri, calculus and OHI-S scores were calculated by Middle East and South Asia regions represented by countries with more than 100 subjects per country. OHI-S and debri scores were significantly higher in Middle East (0.90 and 0.78) subjects than South Asia (0.84 and 0.68, p=0.000). In contrast, Calculus score was higher in South Asia subjects compared to Middle East. CONCLUSIONS: • Middle East subjects averaged significantly higher debri and OHI-S scores when compared to South Asia subjects overall. • The highest debri score average was found in Middle East male (Egypt) and female (Palestine) subjects. • The highest calculus score average was found in South Asia male and female (both Bangladesh) subjects. • The highest OHI-S score average was found in South Asia male (Bangladesh) and female (Pakistan) subjects. Debri and OHI-S scores were lowest for Middle East female Iran subjects.

16.
J Orthod Sci ; 1(1): 11-5, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24987619

RESUMO

BACKGROUND: A review of literature indicates the Arab cephalometric pattern compared to the Caucasian cephalometric pattern is skeletally bimaxillary retrusive, dentally bimaxillary protrusive, and more divergent palatal and mandibular planes. OBJECTIVE: The aim of this study was to clarify the cephalometric features of Emirates adults with Class I malocclusion and pleasing soft tissue profile and to evaluate for gender differences. The null hypothesis tested was no differences in lateral cephalometric measurements as a function of gender. MATERIALS AND METHODS: The lateral cephalometric radiographs of adult Emirati nationals with Class I malocclusion were analyzed in order to characterize an indigenous Class I malocclusion population in the United Arab Emirates. Lateral cephalometric radiographs of 30 males with average age of 24.52±6.09 years and 31 females averaging 23.57±5.52 years were analyzed using Dolphin Imaging software. Twenty-two hard and soft tissue measurements comprised the cephalometric analysis. RESULTS: Only one gender difference was demonstrated out of the 22 cephalometric analysis measurements used in the study; SN-PP mean for females (10.74±3.44 degrees) subjects averaged a 2.3 degree higher mean value than the males (8.43±3.95 degrees, P=0.018). The cephalometric study results were compared to published norms from Steiner and Eastman. CONCLUSIONS: Based upon the conditions of the present study, it may be concluded that adult Emirati males and females seeking orthodontic treatment with Class I malocclusion present similar cephalometric profiles with the exception that measurement SN-PP may be steeper in females than males. Moreover, Emiratis are likely to present greater incisor proclination and protrusion than Caucasians and may be generally considered as more bimaxillary protrusive.

17.
J Orthod Sci ; 1(2): 33, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24987622
18.
PLoS One ; 6(9): e25284, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21980415

RESUMO

Here we identified an evolutionarily highly conserved and ubiquitously expressed protein (C9orf82) that shows structural similarities to the death effector domain of apoptosis-related proteins. RNAi knockdown of C9orf82 induced apoptosis in A-549 and MCF7/casp3-10b lung and breast carcinoma cells, respectively, but not in cells lacking caspase-3, caspase-10 or both. Apoptosis was associated with activated caspases-3, -8, -9 and -10, and inactivation of caspases 10 or 3 was sufficient to block apoptosis in this pathway. Apoptosis upon knockdown of C9orf82 was associated with increased caspase-10 expression and activation, which was required for the generation of an 11 kDa tBid fragment and activation of Caspase-9. These data suggest that C9orf82 functions as an anti-apoptotic protein that modulates a caspase-10 dependent mitochondrial caspase-3/9 feedback amplification loop. We designate this ubiquitously expressed and evolutionarily conserved anti-apoptotic protein Conserved Anti-Apoptotic Protein (CAAP). We also demonstrated that treatment of MCF7/casp3-10b cells with staurosporine and etoposides induced apoptosis and knockdown of CAAP expression. This implies that the CAAP protein could be a target for chemotherapeutic agents.


Assuntos
Proteínas Reguladoras de Apoptose/metabolismo , Mitocôndrias/metabolismo , Proteínas Nucleares/metabolismo , Proteínas/metabolismo , Apoptose/efeitos dos fármacos , Apoptose/genética , Proteínas Reguladoras de Apoptose/genética , Western Blotting , Caspase 10/genética , Caspase 10/metabolismo , Caspase 3/genética , Caspase 3/metabolismo , Caspase 8/genética , Caspase 8/metabolismo , Caspase 9/genética , Caspase 9/metabolismo , Linhagem Celular Tumoral , Etoposídeo/farmacologia , Humanos , Proteínas Nucleares/genética , Proteínas/genética , RNA Interferente Pequeno , Estaurosporina/farmacologia
19.
Methods Enzymol ; 494: 139-58, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21402214

RESUMO

The family Methanosarcinaceae has an expanded repertoire of growth substrates relative to most other methanogenic archaea. Various methylamines, methylated thiols, and methanol can serve as precursors to both methane and carbon dioxide. These compounds are mobilized into metabolism by methyltransferases that use the growth substrate to methylate a cognate corrinoid protein, which in turn is used as a substrate by a second methyltransferase to methylate Coenzyme M (CoM), forming methyl-SCoM, the precursor to both methane and carbon dioxide. Orthologs of the methyltransferases, as well as the small corrinoid proteins, are found in many archaeal and bacterial genomes. Some of these are homologs of the methylamine methyltransferases predicted to require pyrrolysine, an atypical genetically encoded amino acid, for synthesis. As a resource for the study of these sizable families of proteins, we describe here techniques our laboratories have used for the study of methanogen corrinoid-dependent methyltransferases, focusing especially on isolation and assay techniques useful for various activities of components of the methylamine- and methylthiol-dependent CoM methyltransferase systems.


Assuntos
Archaea/enzimologia , Archaea/metabolismo , Metano/metabolismo , Metiltransferases/metabolismo , Proteínas Arqueais/isolamento & purificação , Proteínas Arqueais/metabolismo , Mesna/metabolismo , Methanosarcina barkeri/enzimologia , Methanosarcina barkeri/metabolismo , Metiltransferases/isolamento & purificação
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