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1.
Rev. lab. clín ; 12(3): e40-e46, jul.-sept. 2019. tab
Artigo em Espanhol | LILACS-Express | ID: ibc-ET1-4170

RESUMO

El análisis de ADN circulante a partir de sangre periférica ha demostrado ser de utilidad en campos clínicos tan diferentes como la oncología, los trasplantes y el cribado prenatal. Para su incorporación al laboratorio clínico es necesario asegurar protocolos preanalíticos adecuados, reproducibles y estandarizados. En este documento se pretenden dar unas recomendaciones preanalíticas para la obtención de ADN circulante a partir de sangre periférica. Incluyen el tipo de espécimen, el tipo de tubo de extracción, el modo de centrifugación de la muestra, la extracción del ADN circulante y cuantificación, así como su conservación


Cell-free DNA analysis in peripheral blood has been shown to be useful in oncology, organ transplantation, and prenatal screening. For its introduction into the clinical laboratory, it is necessary to ensure appropriate, reproducible and standardised pre-analytical protocols are in place. The aim of this document is to provide pre-analytical recommendations for obtaining of cell free DNA from peripheral blood. These recommendations include the type of sample and extraction tube, the method of centrifugation, the method for cell free DNA extraction, and measurement and storage conditions

2.
Rev. lab. clín ; 12(1): 38-52, ene.-mar. 2019. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-176973

RESUMO

Este documento describe las causas de error más frecuentes en la medición de marcadores tumorales séricos proteicos en sus diferentes fases: preanalítica, analítica y postanalítica y recomendaciones para detectar y solventar problemas, así como la interpretación de los resultados de los marcadores tumorales en la práctica clínica


This document describes the most frequent causes of error in the measurement of 13 serum protein tumour markers in their different phases: preanalytical, analytical and 14 postanalytic and recommendations to detect and solve problems, as well as the 15 interpretation of the results of the Tumor Markers in clinical practice


Assuntos
Humanos , Biomarcadores Tumorais/análise , Técnicas de Laboratório Clínico/métodos , Neoplasias/diagnóstico , Padrões de Prática Médica , Perfil de Impacto da Doença , Reprodutibilidade dos Testes , Coleta de Amostras Sanguíneas/normas , Preservação de Amostras/métodos
5.
Clin Nutr ; 36(2): 471-476, 2017 04.
Artigo em Inglês | MEDLINE | ID: mdl-26847948

RESUMO

BACKGROUND & AIMS: There is no consensus on the most accurate method to diagnose primary hypolactasia. We aimed to compare the diagnostic accuracy of the new gaxilose test with 2 traditional tests (lactose tolerance test and clinical criteria) for the diagnosis of primary hypolactasia using the C/T-13910 polymorphism as a reference standard. METHODS: Patients with a clinical suspicion of lactose intolerance were subjected to gaxilose tests, shortened lactose tolerance tests, and symptom questionnaires before and after overload with 50 g lactose and after a lactose-free diet. The diagnostic accuracy and degree of agreement and correlation were assessed using a genetic test (C/T-13910 polymorphism) as a reference standard and their respective 95% confidence intervals. RESULTS: Thirty consecutive patients (70% women) participated in the study. The genetic test confirmed the C/T-13910 polymorphism in 11 patients (36.8%). The presence of diarrhoea and the symptom score after lactose overload, along with the tolerance test, were the variables with the highest degree of agreement (κ > 0.60). Area under the ROC curve was >0.82 (p < 0.05), with sensitivity and specificity values of >0.80. However, the gaxilose test obtained lower values: κ, 0.47; area under curve, 0.75 (0.57-0.94); sensitivity, 0.82 (0.55-1); and specificity, 0.68 (0.45-0.92). The multivariate analysis showed an association between the post-overload symptom questionnaire and the results of the genetic test (odds ratio: 1.17; 1.04-1.31; p < 0.01). CONCLUSIONS: The presence of diarrhoea and the symptom score after overload with 50 g lactose showed a higher degree of agreement and diagnostic accuracy for primary hypolactasia than the gaxilose test when the genetic test is used as a reference standard.


Assuntos
Dissacarídeos/urina , Lactase/deficiência , Intolerância à Lactose/diagnóstico , Teste de Tolerância a Lactose , Adulto , Colorimetria , Feminino , Testes Genéticos , Humanos , Lactase/genética , Lactose/metabolismo , Intolerância à Lactose/genética , Masculino , Pessoa de Meia-Idade , Ensaios Clínicos Controlados não Aleatórios como Assunto , Polimorfismo de Nucleotídeo Único , Estudos Prospectivos , Sensibilidade e Especificidade , Inquéritos e Questionários , Adulto Jovem
6.
Autoimmunity ; 49(6): 383-387, 2016 09.
Artigo em Inglês | MEDLINE | ID: mdl-27424781

RESUMO

BACKGROUND: Antinuclear antibody (ANA) testing is useful for screening, diagnosis and follow-up of patients with systemic rheumatic diseases. Indirect immunofluorescence (IIF) on HEp-2 cells is the gold standard for ANA testing. However, ANA have also been detected in patients with different cancer types but without any autoimmune disease. To overcome these shortcomings, different automated solid-phase assays have been developed. AIM: To determine the positive rate of a new ANA detection method (EliA CTD Screen, Phadia, Germany), in CRC patients without systemic rheumatic diseases. Additionally, we compare this method with IIF. MATERIALS AND METHODS: Serum samples were obtained before a colonoscopy procedure in a patient cohort (n = 186) with a high clinical suspicion of CRC. Samples for ANA detection in CRC patients were processed in parallel by IIF on HEp-2 and the solid-phase fluoroenzymeimmunoassay EliA CTD Screen (Phadia, Germany) on the Phadia 250 instrument (Phadia GmbH, Freiburg, Germany). Positive samples by IIF and/or CTD were tested with EliA single ANA assays (Phadia, Germany) on the Phadia 250 instrument (Phadia GmbH, Freiburg, Germany). RESULTS: Forty-five patients diagnosed with CRC were included. Four cases were positive by CTD and 23 by IIF. Of the four positive patients by CTD, two were positive and one indeterminate for anti-dsDNA antibodies. Of the 23 positive by IIF, one patient was positive and another indeterminate for anti-dsDNA antibodies, and a third patient was positive for anti-U1RNP antibodies. CONCLUSIONS: The CTD assay shows a low false positive rate for detecting autoantibodies in a clinical context of CRC.


Assuntos
Autoanticorpos/sangue , Autoanticorpos/imunologia , Neoplasias Colorretais/sangue , Neoplasias Colorretais/imunologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Anticorpos Antinucleares/sangue , Anticorpos Antinucleares/imunologia , Neoplasias Colorretais/diagnóstico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Metástase Neoplásica , Estadiamento de Neoplasias , Estudos Prospectivos
7.
Dig Dis Sci ; 61(1): 208-14, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26320083

RESUMO

BACKGROUND: The lactose tolerance test is a classic method for the study of lactose malabsorption. However, the methodology used has not been standardized, and this leads to differences in results. AIM: The aim of this report was to analyze whether capillary blood glucose measurement is in agreement with venous blood glucose when performing lactose tolerance test. METHODS: This is a prospective study of consecutive patients with suspected lactose malabsorption who had lactose tolerance test performed in venous and capillary blood simultaneously, using a load of 50 g lactose. Agreement was measured using the concordance correlation coefficient of Lin (95 % CI) and Bland-Altman plots. The degree of agreement was measured using the Kappa index. A value of p < 0.05 was considered statistically significant. RESULTS: Eighty-four patients (68 % women) were included. The concordance correlation coefficient showed very poor agreement between the two techniques: 0.68 (0.58-0.77), 0.72 (0.62-0.8), and 0.77 (0.69-0.83) for baseline, 30, and 60 min, respectively. The Bland-Altman plots showed that capillary blood glucose measurements result in higher levels than venous blood glucose measurements, with mean differences of 0.39, 0.77, and 1.1 mmol/L at baseline, 30, and 60 min, respectively. The degree of agreement was low, with a Kappa index of 0.59 (p < 0.001). CONCLUSIONS: The test measured in venous blood is not in agreement with the measurement obtained from capillary blood. It is likely that the diagnostic accuracy attributed without distinction to lactose tolerance test in different studies for lactose malabsorption is incorrect, making it necessary to specify the analysis method.


Assuntos
Glicemia/metabolismo , Capilares , Intolerância à Lactose/diagnóstico , Teste de Tolerância a Lactose/métodos , Extremidade Superior/irrigação sanguínea , Veias , Adulto , Biomarcadores/sangue , Feminino , Dedos/irrigação sanguínea , Humanos , Intolerância à Lactose/sangue , Teste de Tolerância a Lactose/instrumentação , Modelos Lineares , Masculino , Pessoa de Meia-Idade , Variações Dependentes do Observador , Valor Preditivo dos Testes , Estudos Prospectivos , Fitas Reagentes , Reprodutibilidade dos Testes
8.
Anticancer Res ; 35(7): 4271-6, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-26124389

RESUMO

BACKGROUND/AIM: Tumor M2-pyruvate kinase (M2-PK) is up-regulated in proliferating tissues. It has been shown that tumor M2-PK is detectable and quantifiable in the stool and plasma of patients with colorectal cancer (CRC). Tumor M2-PK has been extensively studied in gastrointestinal tumors but its role in other cancer types has not yet been deeply evaluated. The aim of the study was to determine and compare plasma tumor M2-PK levels in different cancer types. MATERIALS AND METHODS: All patients undergoing diagnostics for cancer at our Hospital during 2011 were included in the study (n=139). Plasma tumor M2-PK concentration was analyzed by an enzyme-linked immunosorbent assay. RESULTS: The different cancer types found in the study were: 60 colorectal, 43 breast, 8 lung, 5 prostatic, 4 ovarian and the remaining 19 cases were other uncommon tumor types. Most tumors had high concentrations of tumor M2-PK; prostatic, pharyngeal and testicular tumors had levels lower than or near the cut-off. Plasma tumor M2-PK levels were significantly higher in patients with distant metastases and stage IV by TNM. CONCLUSION: Plasma tumor M2-PK is not a specific marker for CRC and is elevated in many other types of cancers, including breast, lung, ovarian, and thyroid. Small amounts are found in prostatic, pharyngeal and testicular tumors.


Assuntos
Neoplasias/sangue , Piruvato Quinase/sangue , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores Tumorais/sangue , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
9.
Rev Esp Enferm Dig ; 106(6): 381-5, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25361448

RESUMO

INTRODUCTION: Lactose malabsorption (LM) is a very common condition with a high prevalence in our setting. Lactose tolerance test (LTT) is a basic, affordable test for diagnosis that requires no complex technology. It has been recently shown that this test can be shortened to 3 measurements (baseline, 30 min, 60 min) with no impact on final results. The purpose of our study was to assess the feasibility and benefits of LTT simplification and shortening to 30 min, as well as the financial impact entailed. MATERIAL AND METHODS: A multicenter, observational study of consecutive patients undergoing LTT for LM suspicion. Patients received 50 g of lactose following a fasting period of 12 h, and had blood collected from a vein at all 3 time points for the measurement of blood glucose (mg/dl). Differences between the shortened and complete test forms were analyzed using McNemar´s test. A comparison of blood glucose levels between patients with normal and abnormal results was performed using Student´s T-test for independent mean values. Consistency was assessed using the kappa index. A p < 0.05 was considered to be statistically significant. RESULTS: A total of 270 patients (69.6 % females) were included, with a mean age of 39.9 ± 16 years. LTT was abnormal for 151 patients (55.9 %). We observed no statistically significant differences in baseline blood glucose levels between patients with normal and abnormal LTT results (p = 0.13); however, as was to be expected, such differences were obvious for the remaining time points (p < 0.01). Deleting blood glucose measurements at 60 minutes only led to overdiagnose LM (false positive results) in 6 patients (2.22 %), with a kappa index of 0.95 (95 % CI: 0.92-0.99) (p < 0.001) versus the complete test. Suppressing measurements at 60 min would have saved at least € 7,726. CONCLUSION: The shortening of LTT to only 2 measurements (baseline and 30-min) hardly leads to any differences in final results, and would entail savings in time, materials, and personnel.


Assuntos
Intolerância à Lactose/diagnóstico , Teste de Tolerância a Lactose , Adulto , Glicemia , Feminino , Humanos , Masculino , Fatores de Tempo , Adulto Jovem
10.
Rev. esp. enferm. dig ; 106(6): 381-385, jun. 2014. tab
Artigo em Espanhol | IBECS | ID: ibc-127429

RESUMO

Introducción: la malabsorción a la lactosa (ML) es una patología muy frecuente con alta prevalencia en nuestro medio. El test de tolerancia a la lactosa (TTL) es una prueba básica y económica que permite su diagnóstico sin precisar tecnología compleja. Recientemente se ha demostrado que este test puede reducirse a 3 determinaciones (basal, 30 y 60 min) sin afectar al resultado final. El propósito de nuestro estudio fue el valorar la factibilidad y ventajas de poder acortar y simplificar el TTL a 30 min, así como el impacto económico que conllevaría. Material y métodos: estudio multicéntrico y observacional de pacientes consecutivos a los que se les realiza un TTL ante la sospecha de ML. Los enfermos reciben 50 g de lactosa tras 12 h de ayuno y se les realiza extracción de sangre venosa en los 3 tiempos para la medición de la glucemia (mg/dl). La diferencia entre el test reducido y el completo se analizaron con el test de McNemar. La comparación de los niveles de glucemia entre los pacientes con test normal y patológico fue realizada usando el test t-Student para comparación de medias independientes. La concordancia fue evaluada con el índice Kappa. Se consideró p < 0,05 como estadísticamente significativo. Resultados: se incluyeron 270 pacientes (69,6 % mujeres) con edad media de 39,9 ± 16 años. El TTL fue patológico en 151 casos (55,9 %). No observamos diferencias estadísticamente significativas entre las glucemias basales de los pacientes con TTL normal o patológico (p = 0,13), sin embargo, como cabía esperar, estas diferencias fueron evidentes en los demás tiempos (p < 0,01). La eliminación de la determinación de glucemia a los 60 min tan solo sobrevaloró el diagnóstico de ML (falsos positivos) en 6 enfermos (2,22 %), con índice kappa de 0,95 (IC 95 %: 0,92- 0,99) (p < 0.001) respecto al test completo. Si se hubiera suprimido la determinación de los 60 min, se podría haber ahorrado al menos 7.726 euros. Conclusión: el reducir el TTL a 2 determinaciones (basal y 30 min) no implica apenas cambios en el resultado final del test, sin embargo conllevaría un ahorro de tiempo, material y personal (AU)


Introduction: Lactose malabsorption (LM) is a very common condition with a high prevalence in our setting. Lactose tolerance test (LTT) is a basic, affordable test for diagnosis that requires no complex technology. It has been recently shown that this test can be shortened to 3 measurements (baseline, 30 min, 60 min) with no impact on final results. The purpose of our study was to assess the feasibility and benefits of LTT simplification and shortening to 30 min, as well as the financial impact entailed. Material and methods: A multicenter, observational study of consecutive patients undergoing LTT for LM suspicion. Patients received 50 g of lactose following a fasting period of 12 h, and had blood collected from a vein at all 3 time points for the measurement of blood glucose (mg/dl). Differences between the shortened and complete test forms were analyzed using McNemar’s test. A comparison of blood glucose levels between patients with normal and abnormal results was performed using Student’s T-test for independent mean values. Consistency was assessed using the kappa index. A p < 0.05 was considered to be statistically significant. Results: A total of 270 patients (69.6 % females) were included, with a mean age of 39.9 ± 16 years. LTT was abnormal for 151 patients (55.9 %). We observed no statistically significant differences in baseline blood glucose levels between patients with normal and abnormal LTT results (p = 0.13); however, as was to be expected, such differences were obvious for the remaining time points (p < 0.01). Deleting blood glucose measurements at 60 minutes only led to overdiagnose LM (false positive results) in 6 patients (2.22 %), with a kappa index of 0.95 (95 % CI: 0.92-0.99) (p < 0.001) versus the complete test. Suppressing measurements at 60 min would have saved at least € 7,726. Conclusion: The shortening of LTT to only 2 measurements (baseline and 30-min) hardly leads to any differences in final results, and would entail savings in time, materials, and personnel (AU)


Assuntos
Humanos , Masculino , Feminino , Intolerância à Lactose/complicações , Intolerância à Lactose/diagnóstico , Teste de Tolerância a Lactose/métodos , Dietoterapia/métodos , Dietoterapia , Intolerância à Lactose/metabolismo , Teste de Tolerância a Lactose/normas , Teste de Tolerância a Lactose , Glicemia/análise , Teste de Tolerância a Glucose/métodos , Estudos Transversais/métodos
11.
World J Urol ; 31(4): 941-5, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22836230

RESUMO

BACKGROUND: Dhat syndrome (DS) consists of vague somatic symptoms and at times sexual dysfunction which the patient falsely attributes to involuntary emissions of semen outside of sexual relations. OBJECTIVE: Describe and analyse the occurrences of DS in patients attending the clinic and clarify the existence of this condition within the Spanish Urological service. MATERIALS AND METHODS: Patients reporting semen loss in urine or involuntarily outside of sexual relations were studied during a period from May 2006 to December 2007. Variables of age, nationality, marital status, family situation, medical history, reasons for the consultation, physical condition and additional tests were studied. All treatments and its effectiveness were also recorded. RESULTS: DS affected predominantly southern Asian continent citizens (n = 32). The average age was 35.44. Seventeen patients reported semen loss during urination; 20 at the end of urination; 11 spontaneously; 5 while sleeping; 4 during defecation; 1 while showering; 1 while eating meat; and 3 produced by noticing stained clothing. In 28 cases, the supposed loss of semen was linked to sex-related symptoms. All examinations and tests ruled out the existence of actual loss of semen. CONCLUSIONS: In urology consultations, we have been witnessing the unusual appearance of DS, a condition known by psychologists and psychiatrists and practically unheard of by urologists. A previously unknown condition in Spain, immigration from Asia, is causing the appearance of this syndrome. Its rapid identification will prevent patients from paying costly and unnecessary tests and provide alternative therapies, within a multidisciplinary approach involving psychologists and psychiatrists.


Assuntos
Sêmen/metabolismo , Disfunções Sexuais Fisiológicas/epidemiologia , Disfunções Sexuais Psicogênicas/epidemiologia , Doenças Urológicas/epidemiologia , Adolescente , Adulto , Bangladesh/etnologia , Humanos , Masculino , Pessoa de Meia-Idade , Paquistão/etnologia , Prevalência , Estudos Retrospectivos , Disfunções Sexuais Fisiológicas/etnologia , Disfunções Sexuais Fisiológicas/psicologia , Disfunções Sexuais Psicogênicas/etnologia , Disfunções Sexuais Psicogênicas/psicologia , Espanha/epidemiologia , Síndrome , Doenças Urológicas/etnologia , Doenças Urológicas/psicologia , Adulto Jovem
12.
Tumour Biol ; 34(1): 471-9, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23132294

RESUMO

KISS1 is a metastasis suppressor lost in several solid malignancies. We evaluated the clinical relevance of KiSS-1 methylation and its protein expression in colorectal cancer. The epigenetic silencing of KiSS-1 by hypermethylation was tested in colon cancer cells (n = 5) before and after azacytidine treatment. KiSS-1 methylation was evaluated by methylation-specific PCR in colorectal cancer cells, and normal, benign, and tumor tissues (n = 352) were grouped in a training set (n = 62) and two independent validation cohorts (n = 100 and n = 190). KiSS-1 protein expression was analyzed by immunohistochemistry on tissue arrays. KiSS-1 hypermethylation correlated with transcript and protein expression loss, being increased in vitro by azacytidine. Methylation rates were 53.1, 70.0, and 80.0 % in the training and validation sets, respectively. In the training set, KiSS-1 methylation rendered a diagnostic accuracy of 72.7 % (p = 0.002). Combination of KiSS-1 methylation and serum CEA (p = 0.001) increased the prognostic utility of CEA alone (p = 0.022). In the first validation set, KiSS-1 methylation correlated with tumor grade (p = 0.011), predicted recurrence (p = 0.009), metastasis (p = 0.004), disease-free (p = 0.034), and overall survival (p = 0.015). In the second validation cohort, KiSS-1 methylation predicted disease-specific survival (p = 0.030). In the training set, cytoplasmic KiSS-1 expression was significantly higher in nonneoplastic biopsies as compared to colorectal tumors (p < 0.0005). In the validation set, loss of cytoplasmic expression correlated with tumor stage (p = 0.007), grade (p = 0.035), recurrence (p = 0.017), and disease-specific survival (p = 0.022). KiSS-1 was revealed epigenetically modified in colorectal cancer. The diagnostic and prognostic utility of KiSS-1 methylation and expression patterns suggests their assessment for the clinical management of colorectal cancer patients.


Assuntos
Neoplasias Colorretais/genética , Neoplasias Colorretais/metabolismo , Metilação de DNA , Inativação Gênica , Kisspeptinas/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Azacitidina/farmacologia , Biomarcadores Tumorais/sangue , Biomarcadores Tumorais/genética , Linhagem Celular Tumoral , Neoplasias Colorretais/diagnóstico , Progressão da Doença , Epigênese Genética , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Kisspeptinas/metabolismo , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Prognóstico
15.
Tumour Biol ; 33(3): 825-32, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22231432

RESUMO

Colorectal cancer (CRC) can be cured in most cases if diagnosed at an early stage. Carcinoembryonic antigen (CEA) remains the most widely used cancer marker for determining prognosis of CRC. Previous studies have shown that plasmatic tumor M2 pyruvate kinase (Tu M2-PK) is highly sensitive in CRC detection at an early stage and equally as good as the results for established tumor markers with clinical potential for cancer prognosis and monitoring. The aim of this study was to assess the prognostic value of Tu M2-PK in plasma using a survival analysis in combination with CEA in serum in patients newly diagnosed with CRC. The initial study included 183 patients who had a complete diagnostic colonoscopy. This cohort study was designed to evaluate the survival in patients with histologically confirmed gastrointestinal cancers (n = 41). Tu M2-PK concentrations in EDTA plasma were determined immunologically using an ELISA assay. Plasma Tu M2-PK levels were significantly higher in patients with distant metastases, stage IV for TNM score, and advanced stage (C+D) subgroups of Dukes than other subgroups. The univariate Cox's analysis showed that CEA and Tu M2-PK gave high hazard ratios for risk of death (odds ratio CEA = 3.57 and odds ratioTu M2-PK = 2.23) and comparable values in average survival time. The results for both biomarkers did not overlap. These findings suggest that plasmatic Tu M2-PK levels of more than 20 U/mL may be a predictor of death risk.


Assuntos
Antígeno Carcinoembrionário/sangue , Neoplasias Colorretais/diagnóstico , Neoplasias Colorretais/mortalidade , Piruvato Quinase/sangue , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores Tumorais/sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Metástase Neoplásica , Estadiamento de Neoplasias , Prognóstico , Adulto Jovem
16.
Tumour Biol ; 33(2): 337-46, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22252522

RESUMO

Myopodin is an actin-binding protein believed to play a tumor suppressor role in several solid neoplasias. We evaluated the potential differential myopodin methylation and expression and their clinical relevance in colon cancer. The epigenetic silencing of myopodin by hypermethylation was tested in colon cancer cells (n = 5) before and after azacitidine treatment. Myopodin methylation status was evaluated by methylation-specific PCR in colon cancer cells and colorectal tissues (n = 210) grouped in a training set (n = 62) and two independent validation series (n = 100 and n = 48) collected at independent clinical settings. Myopodin expression patterns were analyzed by immunohistochemistry on tissue arrays. Myopodin hypermethylation correlated with gene and protein expression loss, being increased in vitro by azacitidine. Myopodin was frequently methylated in colon cancer cells (four out of five). Methylation rates were 90.3%, 70.0%, and 47.8% in the training and validation sets, respectively. Myopodin methylation rendered a diagnostic accuracy of 83.9% (p < 0.0005). Cytoplasmic myopodin expression was significantly higher in non-neoplastic biopsies compared to colon tumors (p < 0.0005). Loss of myopodin expression correlated with increasing tumor stage (p = 0.011), methylation (p = 0.005), and poor overall survival (p = 0.003). In the first validation set (n = 100), myopodin methylation predicted disease-free (p = 0.046) and overall survival (p = 0.031). In the second validation cohort, myopodin methylation and protein expression patterns predicted disease-specific (p = 0.012 and p = 0.001, respectively) and overall survival (p = 0.009 and p = 0.043, respectively). Thus, myopodin was revealed to be epigenetically modified in colon cancer. The diagnostic and prognostic clinical utility of myopodin methylation and expression patterns suggest considering their assessment for the clinical management of colon cancer patients.


Assuntos
Neoplasias do Colo/genética , Neoplasias do Colo/metabolismo , Perfilação da Expressão Gênica/métodos , Regulação Neoplásica da Expressão Gênica , Proteínas dos Microfilamentos/biossíntese , Proteínas dos Microfilamentos/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Azacitidina/farmacologia , Sequência de Bases , Biomarcadores Tumorais , Neoplasias do Colo/diagnóstico , Ilhas de CpG , Metilação de DNA , Intervalo Livre de Doença , Epigênese Genética , Feminino , Humanos , Imuno-Histoquímica/métodos , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Reação em Cadeia da Polimerase/métodos
17.
Rev. lab. clín ; 4(3): 145-152, jul.-sept. 2011.
Artigo em Espanhol | IBECS | ID: ibc-90887

RESUMO

Objetivo. Evaluar la puesta en marcha de un procedimiento de comunicación de pruebas y valores críticos en el Hospital de Montilla (Empresa Pública Hospital Alto Guadalquivir), Montilla, Córdoba. Material y métodos. Se consensuó un catálogo de «pruebas y valores críticos» estableciendo como óptimo un tiempo de comunicación inferior a 30 minutos. Para identificarlos se crearon en el Sistema Informático de Laboratorio (Omega 3000, Roche Diagnostics) reglas automáticas que generan una prueba de aviso. El valor crítico se comprueba analíticamente conforme al procedimiento. Se establecen las responsabilidades en la comunicación y la relación de personas a avisar en cada supuesto. Para el registro se genera otra prueba que incluye quién comunica, a qué hora, el receptor y la recepción de la notificación («Read-back»). Como indicadores se establecieron: número de comunicaciones, tiempo de demora y efectos adversos por retraso. Resultados. Desde enero a septiembre de 2010 se han realizado 73 avisos por pruebas críticas y 354 por valores críticos (0,64% del total de peticiones) de los que 77 (22%) han correspondido a análisis de rutina y 277 (78%) a análisis de urgencias y consulta única. El aviso más frecuente fue por hiperpotasemia (15,8%). Conclusiones. La puesta en marcha del procedimiento ha generado un ligero aumento de la carga de trabajo en el laboratorio, pero ha supuesto una mayor diligencia clínica a la hora de generar acciones médicas inmediatas. Estas iniciativas generan cultura de seguridad para el paciente y los profesionales, creando sinergias beneficiosas en toda la organización (AU)


Objective. Analysis of running a critical value and a critical test reporting procedure in Montilla Hospital (Empresa Pública Hospital Alto Guadalquivir), Montilla, Córdoba (Spain). Material and method. The concept of critical tests and critical values were defined in a list approved by the physicians and based on the literature. The time of the notification was established as less than 30minutes. To identify critical values, some automatic rules were created to generate a notification test in the Laboratory Informatics System (Omega 3000, Roche Diagnostics). A critical value is checked under the appropriate specific procedure, which also establishes the responsibilities for communication and the priority of persons to be notified in each case. Another test is created to register the notification and must include: who notified, at what time and who received the notification («Read-back»). To control the quality of the process we considered: the number of notifications, the time delay in notifying a critical value and if there had been some adverse effects due to any delay. Results. From January to September in 2010 we have notified 73 critical tests and 354 critical values (0.64% of the analysis applications). We reported 77 (22%) critical values from outpatients and 277 (78%) from inpatients. The most frequent notification was due to hyperkalaemia (15.8%). Conclusions. The procedure has involved a slight increase in laboratory workload, but it has assumed more clinical diligence to make critical decisions. These initiatives generate a safety culture for the patient, the staff and good relationships in the organization (AU)


Assuntos
Masculino , Feminino , Humanos , Análise de Perigos e Pontos Críticos de Controle , Técnicas de Laboratório Clínico/ética , Técnicas de Laboratório Clínico/normas , Técnicas de Laboratório Clínico/tendências , Comunicação , Estudos de Avaliação como Assunto , Avaliação de Processos e Resultados (Cuidados de Saúde)/organização & administração , Avaliação de Processos e Resultados (Cuidados de Saúde)/normas , Avaliação de Processos e Resultados (Cuidados de Saúde) , Avaliação de Programas e Projetos de Saúde/métodos , Avaliação de Programas e Projetos de Saúde/estatística & dados numéricos , Avaliação de Programas e Projetos de Saúde/tendências , Avaliação de Processos e Resultados (Cuidados de Saúde)/métodos
18.
ISRN Rheumatol ; 2011: 780356, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-22389802

RESUMO

We assessed the contribution of four baseline markers-HLA-DRB1 shared epitope (SE), -308 tumor necrosis factor α gene promoter polymorphism, rheumatoid factor, and anticitrullinated peptide antibodies-for predicting persistent activity (DAS28 score ≥2.6) after one year of followup in a cohort of 201 patients with recent-onset rheumatoid arthritis (RA) or undifferentiated arthritis (UA) aged 16 years or older who had a 4-week to 12-month history of swelling of at least two joints. Patients had not been previously treated with corticosteroids or disease-modifying antirheumatic drugs (DMARD). In the best logistic regression model, only two variables were retained: SE positivity and number of DMARD administered (area under the curve = 76.4%; 95% CI: 69.2%, 84.4%; P < 0.001). The best linear regression model also included these two variables, explaining only 22.5% of the variability of DAS28 score. In this study, given an equal number of DMARD administered, the probability of persistent activity in patients with recent-onset RA or UA was significantly influenced by SE presence.

19.
Actas Urol Esp ; 33(8): 888-94, 2009 Sep.
Artigo em Espanhol | MEDLINE | ID: mdl-19900383

RESUMO

OBJECTIVE: The objective of this study is to describe patients referred for urological examination by their primary health care centres according to ethnicity and the disorders in question. The area selected for this study is the Raval Sur neighborhood in the city of Barcelona which has a high immigrant population. This will enable us to understand the health needs of an emerging population which is gaining considerable importance in many Spanish cities, at least where the specialty of urology is concerned. PATIENTS AND METHODS: Prospective study of all patients who visited the urology division at Barcelona's Raval Sur Specialty Centre during 2007. The chosen population consisted of all patients visiting the urology division for the first time. RESULTS: We studied 247 patients, of whom 124 were Spanish, 75 South Asian, 27 Americans, 11 Africans and 10 from other countries. The average age was higher in Spanish patients: 57.86 +/- 17.85 years compared with 37.54 +/- 11.07 years for other nationalities. Both groups were predominantly male. Statistical differences for prostate symptoms were observed between patient groups:, these symptoms were present in 32.4% of Spanish patients, 7.95% of South Asians,17.2% of Americans and 9.1% of Africans. Significant differences were also seen in the PSA increase, affecting 15.2% of Spaniards, with no cases in other groups. Sexual functional pathology was only a motive for examination in the South Asian group, with 56.8% of patients. Phymosis and vasectomy surgery were the reasons for consultation in 9.8% of Spaniards, 2.7% of South Asians, 25% of Americans and were not required by any patient of African origin. Lower back pain was also observed in 0.7% of Spaniards, 1.35% of South Asians and 18.2% of Africans, but in none of the American patients. Gross haematuria was present in 5.1% of Spaniards, 3.4% of Americans, 18.2% of Africans and in no South Asian patients. CONCLUSIONS: The most common reason for consultation in the Spanish patient group was prostatic hypertrophy, American patients requested phymosis surgery or surgical sterilization, and South Asians visited for different sexual pathologies, which affected 56.8% of patients in this group. There were no significant differences in the number ofadditional examinations or in in the number of visits required for diagnosis between the different ethnic groups. One problem that we noted was the missed appointment rate for some patients, which is twice as high in the immigrant group as among Spanish patients and highest in the African and South Asian groups.


Assuntos
Necessidades e Demandas de Serviços de Saúde , Migrantes/estatística & dados numéricos , Doenças Urológicas/terapia , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Espanha
20.
Actas urol. esp ; 33(8): 888-894, sept. 2009. tab
Artigo em Espanhol | IBECS | ID: ibc-84530

RESUMO

Objetivo: El objetivo del presente estudio es describir los tipos de pacientes y patologías por los que son remitidos a la consulta de urología desde las áreas de atención primaria. El área seleccionada para este trabajo es el barrio del Raval Sur, situado en una zona de la ciudad de Barcelona donde se concentra una gran cantidad de inmigrantes. Ello nos permitirá conocer las necesidades sanitarias de este grupo de población emergente que empieza a cobrar una gran importancia en numerosas ciudades españolas, por lo menos en lo referente a la especialidad de urología. Pacientes y métodos: Se estudió, de forma prospectiva, a todos los pacientes que fueron visitados en la consulta de urología del centro de especialidades del Raval Sur de Barcelona durante el año 2007. La población elegida fue toda la que acudió por primera vez a la consulta de urología en dicho período. Resultados: Se estudió a 247 pacientes, de los cuales 124 eran españoles, 75 indostaníes, 27 americanos, 11 africanos y 10 del resto de países. La edad media ± desviación estándar fue superior en los pacientes españoles, con 57,86 ± 17,85 años, frente a los 37,54 ± 11,07 años en el resto de nacionalidades; en ambos grupos predominó el sexo masculino. Se observaron diferencias entre los distintos grupos de pacientes en la sintomatología prostática, que fue del 32,4% en los españoles, el 7,95% de los indostaníes, el 17,2% de los americanos y el 9,1% de los africanos. Igualmente, se observaron diferencias significativas en el aumento del antígeno prostático específico (PSA), con un 15,2% de los españoles, y ningún caso del resto de grupos. La patología funcional de la esfera sexual sólo fue motivo de consulta en el grupo de los pacientes indostaníes, con un 56,8%. La fimosis-vasectomía fue el motivo de consulta en el 9,8% de los españoles, el 2,7% de los indostaníes y el 25% de los americanos, y no fue requerida por ningún paciente de origen africano. En el caso del dolor lumbar, también se observaron diferencias, con un 0,7% de los españoles, un 1,35% de los indostaníes y un 18,2% de los africanos, y no fue la razón de la consulta en ningún paciente americano. La hematuria macroscópica se presentó en el 5,1% de los españoles, el 3,4% de los americanos, el 18,2% de los africanos y en ningún indostaní. Conclusiones: El motivo de consulta más frecuente en el grupo de pacientes españoles fue la sintomatología debida a la hipertrofia prostática; en el grupo de pacientes americanos, la demanda de intervención por fimosis o la esterilización quirúrgica, y los pacientes indostaníes consultan preferentemente por patología múltiple de la esfera sexual, que alcanza el 56,8% en los pacientes de este grupo. No hay grandes diferencias en cuanto al número de exploraciones complementarias o al número de visitas que se necesitaron para el diagnóstico entre las distintas nacionalidades. Uno de los problemas que más se observa es la falta de asistencia a las citas en algunos pacientes, que es el doble en el grupo de pacientes extranjeros frente a los españoles y superior en el grupo de los africanos e indostaníes (AU)


Objective: The objective of this study is to describe patients referred for urological examination by their primary health care centres according to ethnicity and the disorders in question. The area selected for this study is the Raval Sur neighborhood in the city of Barcelona which has a high immigrant population. This will enable us to understand the health needs of an emerging population which is gaining considerable importance in many Spanish cities, at least where the specialty of urology is concerned. Patients and methods: Prospective study of all patients who visited the urology division at Barcelona’s Raval Sur Specialty Centre during 2007. The chosen population consisted of all patients visiting the urology division for the first time. Results: We studied 247 patients, of whom 124 were Spanish, 75 South Asian, 27 Americans, 11 Africans and 10 from other countries. The average age was higher in Spanish patients: 57.86 ± 17.85 years compared with 37.54 ± 11.07 years for other nationalities. Both groups were predominantly male. Statistical differences for prostate symptoms were observed between patient groups:, these symptoms were present in 32.4% of Spanish patients, 7.95%of South Asians,17.2% of Americans and 9.1% of Africans. Significant differences were also seen in the PSA increase, affecting 15.2% of Spaniards, with no cases in other groups. Sexual functional pathology was only a motive for examination in the South Asian group, with 56.8% of patients. Phymosis and vasectomy surgery were the reasons for consultation in 9.8% of Spaniards, 2.7% of South Asians, 25% of Americans and were not required by any patient of African origin. Lower back pain was also observed in 0.7% of Spaniards, 1.35% of South Asians and 18.2% of Africans, but in none of the American patients. Gross haematuria was present in 5.1% of Spaniards, 3.4% of Americans, 18.2% of Africans and in no South Asian patients. Conclusions: The most common reason for consultation in the Spanish patient group was prostatic hypertrophy, American patients requested phymosis surgery or surgical sterilization, and South Asians visited for different sexual pathologies, which affected 56.8% of patients in this group. There were no significant differences in the number of additional examinations or in the number of visits required for diagnosis between the different ethnic groups. One problem that we noted was the missed appointment rate for some patients, which is twice as high in the immigrant group as among Spanish patients and highest in the African and South Asian groups (AU)


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Doenças Urológicas/epidemiologia , Grupos Populacionais/etnologia , Necessidades e Demandas de Serviços de Saúde/tendências , Emigrantes e Imigrantes/estatística & dados numéricos , Fimose/diagnóstico , Fimose/terapia , Análise Estatística , Doenças Urogenitais Masculinas/etnologia , Doenças Urogenitais Femininas/etiologia , Estudos Prospectivos , Atenção Primária à Saúde/métodos
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