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1.
Enferm. clín. (Ed. impr.) ; 30(2): 99-107, mar.-abr. 2020. tab
Artigo em Espanhol | IBECS-Express | ID: ibc-FGT-3072

RESUMO

Objetivo: Evaluar el grado de conocimientos de la hipertensión en pacientes hipertensos. Método: Estudio multicéntrico descriptivo transversal, realizado en centros de atención primaria y especializada de España. Participaron 980 pacientes hipertensos, mayores de 18 años, con tratamiento farmacológico. Variables principales: años de diagnóstico, factores de riesgo, presión arterial, perímetro abdominal, índice de masa corporal, adherencia a la medicación (test de Morisky-Green), y los ítems de un cuestionario validado sobre conocimientos. Resultados: El 50% mujeres, edad media 65 años. El 46% con más de 10 años del diagnóstico de hipertensión. En relación a la adherencia a la medicación, el 56,6% no lo era, siendo el olvido la primera causa. Al analizar los conocimientos sobre la hipertensión el 41,7% refería que nadie les había informado, el 35,8% no consideraba que la hipertensión fuera para toda la vida y solo el 42,3% conocía los objetivos de presión arterial. El mayor desconocimiento sobre los riesgos que provoca la hipertensión en el organismo fue el relacionado con el riñón, donde solo el 48,6% afirmaba conocer ese dato. En relación a la medicación, solo el 41,4% conocía que era para toda la vida. También se observó que hay diferencias estadísticamente significativas entre nivel de conocimientos y nivel de estudios (p < 0,001). Conclusión: Los conocimientos básicos sobre la hipertensión siguen siendo bajos en estos pacientes. Estos resultados permiten orientar futuras intervenciones. orientando la educación terapéutica al paciente y mejorar su participación en la enfermedad para controlar mejor su hipertensión


Aims: To evaluate the grade of knowledge of hypertension in hypertensive patients. Method: Cross-sectional descriptive multicentre study. Primary and specialized care centres in Spain. Participants: 980 hypertensive patients, older than 18 years, with pharmacological treatment. Main variables: Years of diagnosis, risk factors, blood pressure, abdominal circumference, body mass index, adherence to medication (Morisky-Green test) and the items of a validated questionnaire on knowledge. Results: 50% were women, mean age 65 years. Seventy-eight percent lived with family, 46% over 10 years old diagnosed with hypertension. In relation to adherence to medication, 56.6% were not compliant, forgetting being the first cause. Knowledge about hypertension 41.7% said that no one had explained it, 35.8% did not consider hypertension a lifelong process, and only 42.3% knew the objectives of blood pressure. The area of major lack of knowledge of the risk of hypertension was the problems caused by hypertension in the kidney, only 48.6% answered affirmatively. In relation to medication, only 41.4% knew it is for life. Statistically significant differences were also observed between the level of knowledge and the level of studies (p < .001). Conclusions: Basic knowledge about hypertension remains low in these patients. These results enable future interventions to be oriented by promoting therapeutic education to the patient and improving their involvement in the disease in order to better control their hypertension

2.
In Vivo ; 34(2): 715-722, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32111775

RESUMO

BACKGROUND/AIM: There are two strategies for the interpretation of tumor markers (TM) in fluid effusions: i) high cut-off and ii) fluid/serum ratio (F/S) and low cut-off. The objective of this study is to compare these two strategies and to determine whether diagnostic accuracy improves by the identification of possible false positives using Adenosine deaminase (ADA), C reactive protein (CRP) and % of polymorphonuclear cells (%PN). PATIENTS AND METHODS: We studied 157 ascitic fluids, 74 of which were malignant. ADA, CRP and %PN were determined in ascitic fluid, and Carcinoembryonic antigen (CEA), Cancer antigen 72-4 (CA72-4), Cancer antigen CA19-9 and Cancer antigen 15-3 (CA15-3) in both fluid and serum. RESULTS: The strategy of high cut-off showed 59.5% sensitivity at 100% specificity. The F/S strategy showed 75.7% sensitivity at 95.2% specificity. Subclassifying cases with ADA, CRP and %PN negative showed 67.5% sensitivity at 100% specificity for high cut-off and for the F/S strategy was 81.7% sensitivity at 98.7% specificity. CONCLUSION: The strategy of F/S with negative ADA, CRP and %PN allow the best interpretation for TM in the ascitic fluid.

3.
PLoS One ; 15(1): e0227837, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31940415

RESUMO

INTRODUCTION: Snus is an oral tobacco product that originated in Sweden. Snus products are available as fine-cut loose tobacco or in pre-portioned porous "pouches." Some snus products undergo tobacco pasteurization during manufacturing, a process that removes or reduces nitrite-forming microbes, resulting in less tobacco-specific nitrosamine content in the product. Some tobacco companies and researchers have suggested that snus is potentially less harmful than traditional tobacco and thus a potential smoking cessation aid or an alternative to continued cigarette consumption. Although snus is available in various countries, limited information exists on snus variants from different manufacturers. METHODS: Moisture, pH, nicotine, and tobacco-specific N'-nitrosamines (TSNAs) were quantified in 64 snus products made by 10 manufacturers in the United States and Northern Europe (NE). Reported means, standard errors, and differences are least-square (LS) estimates from bootstrapped mixed effects models, which accounted for correlation among repeated measurements. Minor alkaloids and select flavors were also measured. RESULTS: Among all product types, moisture (27.4%-59.5%), pH (pH 5.87-9.10), total nicotine (6.81-20.6 mg/g, wet), unprotonated nicotine (0.083-15.7 mg/g), and total TSNAs (390-4,910 ng/g) varied widely. The LS-mean unprotonated nicotine concentration of NE portion (7.72 mg/g, SE = 0.963) and NE loose (5.06 mg/g, SE = 1.26) snus were each significantly higher than US portion snus (1.00 mg/g, SE = 1.56). Concentrations of minor alkaloids varied most among products with the highest total nicotine levels. The LS-mean NNN+NNK were higher in snus sold in the US (1360 ng/g, SE = 207) than in NE (836 ng/g, SE = 132) countries. The most abundant flavor compounds detected were pulegone, eucalyptol, and menthol. CONCLUSION: Physical and chemical characteristics of US and NE products labeled as snus can vary considerably and should not be considered "equivalent". Our findings could inform public health and policy decisions pertaining to snus exposure and potential adverse health effects associated with snus.

4.
Methods Mol Biol ; 2122: 269-282, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31975309

RESUMO

Isolated microspore culture is the most efficient technique among those used to induce microspore embryogenesis. In the particular case of Brassica napus, it is also the most widely used and optimized. In this chapter, we describe a protocol for microspore culture in B. napus which includes the steps necessary to isolate and culture microspores, to induce microspore-derived embryos, to produce doubled haploid plants from them, as well as to check for the developmental stage of the microspores isolated, their viability, and the ploidy level of regenerated plantlets.

5.
N Engl J Med ; 382(8): 697-705, 2020 02 20.
Artigo em Inglês | MEDLINE | ID: mdl-31860793

RESUMO

BACKGROUND: The causative agents for the current national outbreak of electronic-cigarette, or vaping, product use-associated lung injury (EVALI) have not been established. Detection of toxicants in bronchoalveolar-lavage (BAL) fluid from patients with EVALI can provide direct information on exposure within the lung. METHODS: BAL fluids were collected from 51 patients with EVALI in 16 states and from 99 healthy participants who were part of an ongoing study of smoking involving nonsmokers, exclusive users of e-cigarettes or vaping products, and exclusive cigarette smokers that was initiated in 2015. Using the BAL fluid, we performed isotope dilution mass spectrometry to measure several priority toxicants: vitamin E acetate, plant oils, medium-chain triglyceride oil, coconut oil, petroleum distillates, and diluent terpenes. RESULTS: State and local health departments assigned EVALI case status as confirmed for 25 patients and as probable for 26 patients. Vitamin E acetate was identified in BAL fluid obtained from 48 of 51 case patients (94%) in 16 states but not in such fluid obtained from the healthy comparator group. No other priority toxicants were found in BAL fluid from the case patients or the comparator group, except for coconut oil and limonene, which were found in 1 patient each. Among the case patients for whom laboratory or epidemiologic data were available, 47 of 50 (94%) had detectable tetrahydrocannabinol (THC) or its metabolites in BAL fluid or had reported vaping THC products in the 90 days before the onset of illness. Nicotine or its metabolites were detected in 30 of 47 of the case patients (64%). CONCLUSIONS: Vitamin E acetate was associated with EVALI in a convenience sample of 51 patients in 16 states across the United States. (Funded by the National Cancer Institute and others.).


Assuntos
Lesão Pulmonar Aguda/patologia , Líquido da Lavagem Broncoalveolar/química , Sistemas Eletrônicos de Liberação de Nicotina , Vaping/efeitos adversos , Vitamina E/análise , Lesão Pulmonar Aguda/etiologia , Adolescente , Adulto , Idoso , Fumar Cigarros , Óleo de Coco/análise , Feminino , Humanos , Limoneno/análise , Masculino , Pessoa de Meia-Idade , Estados Unidos , Adulto Jovem
6.
Dermatol Ther ; 32(6): e13115, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31646716

RESUMO

A 42-year-old female with a personal history of Grade II infiltrating ductal breast carcinoma, treated under surgical procedure in 2018, whose hormonal profile included estrogen, progesteronic and HER-2 positive receptors, and a Ki67 proliferative index (20-25%), was referred to our dermatological outpatient clinic complaining worsening of her chronic spontaneous urticaria. The patient was under treatment with etuximide, exemestane, anastrozole, and trastuzumab. Our patient reported intermittent episodes of hives of 8 years of evolution with good control with second-generation antihistamines. However, in the past year, the intensity and frequency of the outbreaks was increased with the development of angioedema until completing 23 consultations through the emergency department being treated in all of the episodes with short cycles of oral corticosteroids. This case reported is particularly complex due to different points that we develop below: (a) oncological patient as special population; (b) differential diagnosis with other forms of urticaria such as vasculitis urticaria, inducible urticaria, and autoimmune progesterone dermatitis; (c) spontaneous chronic urticaria of difficult control at labeled dose of omalizumab; (d) concomitant treatment with another biological drug.

7.
Anticancer Res ; 39(9): 5071-5076, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31519617

RESUMO

BACKGROUND/AIM: Approximately 20% of pleural effusions are associated with cancer; about 50% require invasive procedures to perform diagnosis. Determination of the concentration of soluble cytokeratin 19-fragments (CYFRA21-1) may help identify patients with malignant effusions. However, pathologies other than cancer can increase its concentration. The identification of these possible false positives with routine tests CRP, ADA, % polymorphonuclear cells (PN) may improve diagnostic accuracy. This study aimed to determine the diagnostic accuracy of CYFRA21-1 in the detection of malignant pleural effusions and the possible false positives. MATERIALS AND METHODS: Analysis of CYFRA21-1, adenosine deaminase (ADA), C-reactive protein (CRP), and the percentage of polymorphonuclear leukocytes (PN%) in the fluid from 643 consecutive undiagnosed pleural effusions was performed. RESULTS: CYFRA21-1 showed 38.7% sensitivity and 97.3% specificity at 175 ng/ml cut-off. Effusions not suspicious of a false-positive showed 39.0% sensitivity and 98.2% specificity, while effusions suspicious of false positive showed lower sensitivity (36.4%) and specificity (95.0%). CONCLUSION: The diagnostic accuracy of CYFRA21-1 in pleural effusions can be improved by classification according to the possibility of false positives.


Assuntos
Antígenos de Neoplasias/metabolismo , Biomarcadores Tumorais , Queratina-19/metabolismo , Derrame Pleural/diagnóstico , Derrame Pleural/metabolismo , Biópsia , Feminino , Humanos , Masculino , Derrame Pleural/etiologia , Curva ROC , Valores de Referência , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
8.
J Fish Dis ; 42(6): 883-894, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30950084

RESUMO

Two aqueous fixation methods (modified Davidson's solution and modified Davidson's solution with 2% (w/v) Alcian blue) were compared against two non-aqueous fixation methods (methacarn solution and methacarn solution with 2% (w/v) Alcian blue) along with the standard buffered formalin fixation method to (a) improve preservation of the mucous coat on Atlantic salmon, Salmo salar L., gills and (b) to examine the interaction between the amoebae and mucus on the gill during an infection with amoebic gill disease. Aqueous fixatives demonstrated excellent cytological preservation but failed to deliver the preservation of the mucus when compared to the non-aqueous-based fixatives; qualitative and semi-quantitative analysis revealed a greater preservation of the gill mucus using the non-aqueous methacarn solution. A combination of this fixation method and an Alcian blue/Periodic acid-Schiff staining was tested in gills of Atlantic salmon infected with amoebic gill disease; lectin labelling was also used to confirm the mucus preservation in the methacarn-fixed tissue. Amoebae were observed closely associated with the mucus demonstrating that the techniques employed for preservation of the mucous coat can indeed avoid the loss of potential mucus-embedded parasites, thus providing a better understanding of the relationship between the mucus and parasite.


Assuntos
Ácido Acético/química , Clorofórmio/química , Brânquias/parasitologia , Metanol/química , Muco , Salmo salar/parasitologia , Fixação de Tecidos/métodos , Azul Alciano/química , Amoeba/patogenicidade , Animais , Fixadores/química , Formaldeído/química , Brânquias/patologia , Reação do Ácido Periódico de Schiff , Salmo salar/anatomia & histologia
9.
Enferm Clin ; 2019 Apr 03.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-30954394

RESUMO

AIMS: To evaluate the grade of knowledge of hypertension in hypertensive patients. METHOD: Cross-sectional descriptive multicentre study. Primary and specialized care centres in Spain. PARTICIPANTS: 980 hypertensive patients, older than 18 years, with pharmacological treatment. MAIN VARIABLES: Years of diagnosis, risk factors, blood pressure, abdominal circumference, body mass index, adherence to medication (Morisky-Green test) and the items of a validated questionnaire on knowledge. RESULTS: 50% were women, mean age 65 years. Seventy-eight percent lived with family, 46% over 10 years old diagnosed with hypertension. In relation to adherence to medication, 56.6% were not compliant, forgetting being the first cause. Knowledge about hypertension 41.7% said that no one had explained it, 35.8% did not consider hypertension a lifelong process, and only 42.3% knew the objectives of blood pressure. The area of major lack of knowledge of the risk of hypertension was the problems caused by hypertension in the kidney, only 48.6% answered affirmatively. In relation to medication, only 41.4% knew it is for life. Statistically significant differences were also observed between the level of knowledge and the level of studies (p<.001). CONCLUSIONS: Basic knowledge about hypertension remains low in these patients. These results enable future interventions to be oriented by promoting therapeutic education to the patient and improving their involvement in the disease in order to better control their hypertension.

10.
Front Plant Sci ; 10: 242, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30915089

RESUMO

Zea mays Brittle1-1 (ZmBT1-1) is an essential component of the starch biosynthetic machinery in maize endosperms, enabling ADPglucose transport from cytosol to amyloplast in exchange for AMP or ADP. Although ZmBT1-1 has been long considered to be an amyloplast-specific marker, evidence has been provided that ZmBT1-1 is dually localized to plastids and mitochondria (Bahaji et al., 2011b). The mitochondrial localization of ZmBT1-1 suggested that this protein may have as-yet unidentified function(s). To understand the mitochondrial ZmBT1-1 function(s), we produced and characterized transgenic Zmbt1-1 plants expressing ZmBT1-1 delivered specifically to mitochondria. Metabolic and differential proteomic analyses showed down-regulation of sucrose synthase (SuSy)-mediated channeling of sucrose into starch metabolism, and up-regulation of the conversion of sucrose breakdown products generated by cell wall invertase (CWI) into ethanol and alanine, in Zmbt1-1 endosperms compared to wild-type. Electron microscopic analyses of Zmbt1-1 endosperm cells showed gross alterations in the mitochondrial ultrastructure. Notably, the protein expression pattern, metabolic profile, and aberrant mitochondrial ultrastructure of Zmbt1-1 endosperms were rescued by delivering ZmBT1-1 specifically to mitochondria. Results presented here provide evidence that the reduced starch content in Zmbt1-1 endosperms is at least partly due to (i) mitochondrial dysfunction, (ii) enhanced CWI-mediated channeling of sucrose into ethanol and alanine metabolism, and (iii) reduced SuSy-mediated channeling of sucrose into starch metabolism due to the lack of mitochondrial ZmBT1-1. Our results also strongly indicate that (a) mitochondrial ZmBT1-1 is an important determinant of the metabolic fate of sucrose entering the endosperm cells, and (b) plastidic ZmBT1-1 is not the sole ADPglucose transporter in maize endosperm amyloplasts. The possible involvement of mitochondrial ZmBT1-1 in exchange between intramitochondrial AMP and cytosolic ADP is discussed.

11.
J Exp Bot ; 70(4): 1267-1281, 2019 02 20.
Artigo em Inglês | MEDLINE | ID: mdl-30715473

RESUMO

Microspore embryogenesis is an experimental morphogenic pathway with important applications in basic research and applied plant breeding, but its genetic, cellular, and molecular bases are poorly understood. We applied a multidisciplinary approach using confocal and electron microscopy, detection of Ca2+, callose, and cellulose, treatments with caffeine, digitonin, and endosidin7, morphometry, qPCR, osmometry, and viability assays in order to study the dynamics of cell wall formation during embryogenesis induction in a high-response rapeseed (Brassica napus) line and two recalcitrant rapeseed and eggplant (Solanum melongena) lines. Formation of a callose-rich subintinal layer (SL) was common to microspore embryogenesis in the different genotypes. However, this process was directly related to embryogenic response, being greater in high-response genotypes. A link could be established between Ca2+ influx, abnormal callose/cellulose deposition, and the genotype-specific embryogenic competence. Callose deposition in inner walls and SLs are independent processes, regulated by different callose synthases. Viability and control of internal osmolality are also related to SL formation. In summary, we identified one of the causes of recalcitrance to embryogenesis induction: a reduced or absent protective SL. In responding genotypes, SLs are markers for changes in cell fate and serve as osmoprotective barriers to increase viability in imbalanced in vitro environments. Genotype-specific differences relate to different responses against abiotic (heat/osmotic) stresses.

12.
Curr Psychiatry Rep ; 20(8): 66, 2018 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-30069650

RESUMO

PURPOSE OF REVIEW: The aim of the present review is to systematically examine published data regarding ecological momentary assessment (EMA) in children and adolescents with mood disorders. RECENT FINDINGS: EMA is increasingly used to collect participant's information in their real environment and in real time. There are multiple studies focused on the evaluation of mood disorders in children and adolescents, but only a few of them used EMA protocols. Results found in this review showed a wide variability of works with different fields of study, methodological approaches, and EMA protocols. More than 60% of EMA studies in children and adolescents with mood disorders were conducted via phone call, showing high completion rates with data missing in 5 to 11.5% of the calls. Length of studies varied from a 4-day EMA protocol to a maximum of 8 weeks. Positive and negative affect, daily activities, and social context were the main EMA measures. Despite the limited number of studies using EMA in children and adolescents with mood disorders, EMA was useful in assessing mood symptoms in the moment and in patients' real-life environment. Studies also showed high completion and satisfaction rates. Although web pages and apps use have been increasing over the past years, the evidence base is still scarce. Future studies can facilitate understanding of EMA methodology among youth with mood disorders.


Assuntos
Afeto , Avaliação Momentânea Ecológica , Transtornos do Humor/psicologia , Adolescente , Criança , Humanos , Meio Social
13.
Atten Defic Hyperact Disord ; 10(4): 247-265, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30132248

RESUMO

Ecological momentary assessment is an excellent tool for the measurement of different day-to-day domains in patients and capturing real-world and real-time data. The purpose of this review is to evaluate feasibility in current ecological momentary assessment studies on emotional and behavioral functioning, functional impairments, and quality of life patients with an attention-deficit/hyperactivity disorder diagnosis. This systematic review follows the recommendation of Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines selecting articles published from January 1, 1990, up to the latest access on May 2018, identifying a pool of 23 eligible studies. Twenty-three studies demonstrate the validity of ecological momentary assessment methodology in evaluating different aspects of patients with attention-deficit/hyperactivity disorder. Fifteen studies focus on the child's or adolescent's daily behavior, while eight studies only focus on adults. The studies presented in this review monitored patients and their families over a maximum period of 28 days. We can conclude that ecological momentary assessment can be successfully implemented with attention-deficit/hyperactivity disorder patients to evaluate diverse backgrounds. However, more studies are needed with a longer monitoring period, especially in adolescents, to determine the effectiveness of ecological momentary assessment on patients with attention-deficit/hyperactivity disorder.


Assuntos
Transtorno do Deficit de Atenção com Hiperatividade/diagnóstico , Transtorno do Deficit de Atenção com Hiperatividade/psicologia , Avaliação Momentânea Ecológica , Adolescente , Criança , Humanos
14.
Reumatol. clín. (Barc.) ; 14(4): 191-195, jul.-ago. 2018. tab
Artigo em Espanhol | IBECS | ID: ibc-175920

RESUMO

Objetivo: Analizar la evolución a largo plazo de la calidad de vida relacionada con la salud (CVRS) en pacientes con artritis reumatoide (AR) tratados con terapias biológicas. Método: Estudio observacional prospectivo realizado entre octubre de 2006 y mayo de 2011. Se seleccionó a pacientes mayores de edad, diagnosticados de AR, tratados durante al menos un año con anti-TNF (infliximab o etanercept) y que no hubieran recibido otros tratamientos biológicos previos. Completaron el estudio 41 pacientes, cumplimentando el cuestionario específico validado QOL-AR en 3ocasiones: E1 (entre septiembre de 2006 y febrero de 2007), E2 (entre abril de 2008 y enero de 2009) y E3 (entre julio de 2010 y mayo de 2011). Los datos se analizaron con Epi-Info versión 3.3-2004 para Windows (R) y Excel 2007; la comparación de las medias con la prueba t de Student y la relación entre valores de un mismo paciente, mediante regresión lineal. Resultados: Resultados globales: tendencia descendente no estadísticamente significativa: 7,09±1,15 en E1; 6,90±1,60 en E2 y 6,52±1,59 en E3. Los ítems con mayor puntuación fueron los relacionados con aspectos psicosociales (ayuda familiar, interacción con familia y amigos). La dimensión física fue la peor valorada (habilidad física, dolor artrítico, artritis). Entre E2 y E3 aumentó significativamente la valoración de la ayuda familiar (p=0,0008) y disminuyeron significativamente tensión nerviosa (p=0,0119) y estado de ánimo (p=0,0451). Conclusiones: La CVRS de los pacientes es buena y se ha mantenido prácticamente invariable tras unos 6años de estudio. Es probable que parte de la estabilidad en la CVRS sea atribuible al tratamiento


Objective: To analyze the changes in health-related quality of life (HRQoL) of patients with rheumatoid arthritis (RA) treated with biological therapies. Method: Observational prospective study performed from October 2006 to May 2011. The inclusion criteria were adult patients, diagnosed with RA, treated for at least one year with anti-tumor necrosis factor therapy (infliximab or etanercept), who had not received other biological treatments previously. A total of 41 patients who completed the study undertook the specific and validated questionnaire QoL-RA Scale 3 times: E1 (September 2006-February 2007), E2 (April 2008-January 2009) and E3 (July 2010- May 2011). Data analysis was conducted using Epi-Info version 3.3 2004 for Windows(R) and Excel 2007; mean comparisons were evaluated by Student's t-test and the relationship between the 3 outcomes for each patient by lineal regression. Results: Overall results show a downward trend which was not statistically significant: 7.09 (standard deviation [SD]=1.15) in E1; 6.90 (SD=1.60) in E2; and 6.52 (SD=1.59) in E3. Items with higher scores were those related to psychosocial aspects (help from family, interaction with family and friends), whereas the physical dimension was valued more poorly (physical ability, arthritis pain, arthritis). Between E2 and E3 there was a significant increase in help from family (P=.0008), whereas level of tension (P=.0119) and mood (P=.0451) decreased significantly. Conclusions: In all, HRQoL reported by patients is good and has remained unchanged after approximately 6 years of study. The stability of HRQoL is probably partly attributable to treatment


Assuntos
Humanos , Masculino , Feminino , Artrite Reumatoide/tratamento farmacológico , Terapia Biológica , Doenças Autoimunes/tratamento farmacológico , Tempo , Qualidade de Vida , Estudos Prospectivos , Etanercepte/farmacocinética , Infliximab/farmacocinética , Psicometria/instrumentação
15.
Rev. neurol. (Ed. impr.) ; 66(supl.2): S57-S60, 5 jun., 2018.
Artigo em Espanhol | IBECS | ID: ibc-175393

RESUMO

En cuidados paliativos, es necesario evaluar y responder a las necesidades psicológicas y espirituales de los participantes para conseguir el máximo bienestar posible tanto del paciente como de su familia. Afrontar la propia muerte, la pérdida de un ser querido o la pérdida de algún aspecto significativo en la vida de una persona supone uno de los retos más difíciles que afronta el ser humano. La muerte como expresión máxima de pérdida es vivida como una amenaza, como uno de los acontecimientos más difíciles de afrontar y con mayor impacto psicológico. En la etapa final de la vida, el paciente se enfrenta a un importante impacto emocional, a muchas pérdidas y a afrontar los miedos que todo esto provoca. No se elige este camino, pero sí puede elegir la actitud en el modo de andar ese camino de forma que aumente su percepción de control y disminuya la incertidumbre y la sensación de imprevisibilidad. Se presentan las necesidades psicológicas y espirituales, así como aspectos relacionados con el afrontamiento de la familia de un niño que padece una enfermedad grave y recibe cuidados paliativos


In palliative care it is necessary to evaluate and respond to the psychological and spiritual needs of the participants in order to achieve the maximum possible well-being for both the patient and his/her family. Coping with one's own death, the loss of a loved one or the loss of some significant aspect in a person’s life is one of the most difficult challenges that human beings have to face. Death, as the maximum expression of loss, is experienced as a threat, as one of the most difficult events to cope with and with the greatest psychological impact. In the final stage of life, the patient has to deal with an important emotional impact, many losses and the need to cope with the fears that the whole situation triggers.This path cannot be chosen, but it is possible to choose the attitude taken towards the way the path is walked, so that the npatient's perception of control increases, while the sense of uncertainty and the unforeseeable nature of the situation are reduced. This article reports on the psychological and spiritual needs of the family of a child suffering from a severe disease and receiving palliative care, as well as addressing aspects related to the way they cope with such circumstances


Assuntos
Humanos , Criança , Família/psicologia , Espiritualidade , Determinação de Necessidades de Cuidados de Saúde , Cuidados Paliativos , Índice de Gravidade de Doença
16.
Comput Math Methods Med ; 2018: 7353624, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29770158

RESUMO

WHODAS 2.0 is the standard measure of disability promoted by World Health Organization whereas Clinical Global Impression (CGI) is a widely used scale for determining severity of mental illness. Although a close relationship between these two scales would be expected, there are no relevant studies on the topic. In this study, we explore if WHODAS 2.0 can be used for identifying severity of illness measured by CGI using the Fisher Linear Discriminant Analysis (FLDA) and for identifying which individual items of WHODAS 2.0 best predict CGI scores given by clinicians. One hundred and twenty-two patients were assessed with WHODAS 2.0 and CGI during three months in outpatient mental health facilities of four hospitals of Madrid, Spain. Compared with the traditional correction of WHODAS 2.0, FLDA improves accuracy in near 15%, and so, with FLDA WHODAS 2.0 classifying correctly 59.0% of the patients. Furthermore, FLDA identifies item 6.6 (illness effect on personal finances) and item 4.5 (damaged sexual life) as the most important items for clinicians to score the severity of illness.


Assuntos
Avaliação da Deficiência , Pessoas com Deficiência/classificação , Transtornos Mentais/diagnóstico , Organização Mundial da Saúde , Adolescente , Humanos , Transtornos Mentais/classificação , Reprodutibilidade dos Testes , Índice de Gravidade de Doença , Espanha , Adulto Jovem
17.
Plant Methods ; 14: 30, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29686723

RESUMO

Background: For in vitro culture of plant and animal cells, one of the critical steps is to adjust the initial cell density. A typical example of this is isolated microspore culture, where specific cell densities have been determined for different species. Out of these ranges, microspore growth is not induced, or is severely reduced. A similar situation occurs in many other plant and animal cell culture systems. Traditionally, researchers have used counting chambers (hemacytometers) to calculate cell densities, but little is still known about their technical advantages. In addition, much less information is available about other, alternative methods. In this work, using isolated eggplant microspore cultures and fluorescent beads (fluorospheres) as experimental systems, we performed a comprehensive comparison of six methods to calculate cell densities: (1) a Neubauer improved hemacytometer, (2) an automated cell counter, (3) a manual-counting method, and three flow cytometry methods based on (4) autofluorescence, (5) propidium iodide staining, and (6) side scattered light (SSC). Results: Our results show that from a technical perspective, hemacytometers are the most reasonable option for cell counting, which may explain their widely spread use. Automated cell counters represent a good compromise between precision and affordability, although with limited accuracy. Finally, the methods based on flow cytometry were, by far, the best in terms of reproducibility and agreement between them, but they showed deficient accuracy and precision. Conclusions: Together, our results show a thorough technical evaluation of each counting method, provide unambiguous arguments to decide which one is the most convenient for the particular case of each laboratory, and in general, shed light into the best way to determine cell densities for in vitro cell cultures. They may have an impact in such a practice not only in the context of microspore culture, but also in any other plant cell culture procedure, or in any process involving particle counting.

18.
Reumatol Clin ; 14(4): 191-195, 2018.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-28256445

RESUMO

OBJECTIVE: To analyze the changes in health-related quality of life (HRQoL) of patients with rheumatoid arthritis (RA) treated with biological therapies. METHOD: Observational prospective study performed from October 2006 to May 2011. The inclusion criteria were adult patients, diagnosed with RA, treated for at least one year with anti-tumor necrosis factor therapy (infliximab or etanercept), who had not received other biological treatments previously. A total of 41 patients who completed the study undertook the specific and validated questionnaire QoL-RA Scale 3 times: E1 (September 2006-February 2007), E2 (April 2008-January 2009) and E3 (July 2010- May 2011). Data analysis was conducted using Epi-Info version 3.3 2004 for Windows® and Excel 2007; mean comparisons were evaluated by Student's t-test and the relationship between the 3 outcomes for each patient by lineal regression. RESULTS: Overall results show a downward trend which was not statistically significant: 7.09 (standard deviation [SD]=1.15) in E1; 6.90 (SD=1.60) in E2; and 6.52 (SD=1.59) in E3. Items with higher scores were those related to psychosocial aspects (help from family, interaction with family and friends), whereas the physical dimension was valued more poorly (physical ability, arthritis pain, arthritis). Between E2 and E3 there was a significant increase in help from family (P=.0008), whereas level of tension (P=.0119) and mood (P=.0451) decreased significantly. CONCLUSIONS: In all, HRQoL reported by patients is good and has remained unchanged after approximately 6 years of study. The stability of HRQoL is probably partly attributable to treatment.


Assuntos
Antirreumáticos/uso terapêutico , Artrite Reumatoide/tratamento farmacológico , Etanercepte/uso terapêutico , Infliximab/uso terapêutico , Qualidade de Vida , Adulto , Idoso , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Resultado do Tratamento
19.
Rev. chil. neuro-psiquiatr ; 56(4): 219-227, 2018. graf
Artigo em Espanhol | LILACS-Express | ID: biblio-990861

RESUMO

Resumen Introducción: La prevalencia de depresion y ansiedad en usuarios internados en hospitales generales es muy alta, esto se asocia a mayor morbimortalidad y mayores costos en salud. El apoyo emocional resulta fundamental en todo proceso de recuperación. Método: Se realizó un estudio descriptivo y analítico sobre depresión y su vinculación con el apoyo social, estancia hospitalaria y enfermedad médica en pacientes internados en el Hospital de Clínicas Dr. Manuel Quíntela Montevideo, Uruguay. Se les aplicó el test de Beck para depresión y la escala validada para soporte social Medical Outcomes Study (MOS). Se describieron características de la población estudiada. Resultados: Más de la mitad de los entrevistados presentaban algún grado de depesión. Fue posible afirmar que 73,7% de los usuarios con apoyo social escaso presentó síntomas depresivos con un aumento de la depresión sobre todo de tipo moderada y leve. Hubo una mayor incidencia de depresión en pacientes con estancia hospitalaria prolongada, sin embargo, este dato no fue estadísticamente significativo. En cuanto a la patología médica destacamos la fuerte presencia de depresión en los pacientes oncológicos cardiovasculares y quirúrgicos.


Introduction: The prevalence of depression and anxiety in pacients hospitalized in general hospitals is very high, this is associated with increased morbidity and mortality and higher health costs. Emotional support is essential in any recovery process. Method: A descriptive and analytical study was carried out on depression and its relationship with social support, hospital stay and medical illness in patients admitted to the Dr. Manuel Quintela Clinical Hospital Montevideo - Uruguay. The Beck test for depression and the scale validated for social support Medical Outcomes Study (MOS) were applied. Characteristics of the studied population were described. Results: More than half of the interviewees presented some degree of depesión. It was possible to affirm that 73.7% of the users with scarce social support presented depressive symptoms with an increase in depression, especially of moderate and mild type. There was a higher incidence of depression in patients with prolonged hospital stay, however this data was not statistically significant. Regarding medical pathology, we highlight the strong presence of depression in cardiovascular and surgical oncology patients.

20.
Genome Announc ; 5(18)2017 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-28473380

RESUMO

Lysobacter enzymogenes strain B25, an isolate from an agricultural field, acts as a biological control agent against root knot nematodes in tomato plants. B25 also controls several fungal diseases and promotes plant growth under abiotic stress. We hereby report on the draft genome sequence and assembly of B25.

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