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1.
PLoS One ; 11(4): e0154253, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27119555

RESUMO

CD11c is an α integrin classically employed to define myeloid dendritic cells. Although there is little information about CD11c expression on human T cells, mouse models have shown an association of CD11c expression with functionally relevant T cell subsets. In the context of genital tract infection, we have previously observed increased expression of CD11c in circulating T cells from mice and women. Microarray analyses of activated effector T cells expressing CD11c derived from naïve mice demonstrated enrichment for natural killer (NK) associated genes. Here we find that murine CD11c+ T cells analyzed by flow cytometry display markers associated with non-conventional T cell subsets, including γδ T cells and invariant natural killer T (iNKT) cells. However, in women, only γδ T cells and CD8+ T cells were enriched within the CD11c fraction of blood and cervical tissue. These CD11c+ cells were highly activated and had greater interferon (IFN)-γ secretory capacity than CD11c- T cells. Furthermore, circulating CD11c+ T cells were associated with the expression of multiple adhesion molecules in women, suggesting that these cells have high tissue homing potential. These data suggest that CD11c expression distinguishes a population of circulating T cells during bacterial infection with innate capacity and mucosal homing potential.


Assuntos
Antígeno CD11c/imunologia , Infecções por Chlamydia/imunologia , Chlamydia muridarum/imunologia , Ativação Linfocitária , Subpopulações de Linfócitos T/imunologia , Vaginose Bacteriana/imunologia , Adulto , Animais , Antígenos CD/imunologia , Antígenos Ly/sangue , Antígenos Ly/imunologia , Movimento Celular , Infecções por Chlamydia/sangue , Feminino , Humanos , Cadeias alfa de Integrinas/imunologia , Interferon gama/imunologia , Camundongos , Camundongos Endogâmicos C57BL , Pessoa de Meia-Idade , Subfamília B de Receptores Semelhantes a Lectina de Células NK/sangue , Subfamília B de Receptores Semelhantes a Lectina de Células NK/imunologia , Células T Matadoras Naturais/imunologia , Receptores de Antígenos de Linfócitos T gama-delta/imunologia , Vaginose Bacteriana/sangue
2.
PLoS One ; 10(11): e0142186, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26555789

RESUMO

CD26 is a T cell activation marker consisting in a type II transmembrane glycoprotein with dipeptidyl peptidase IV (DPPIV) activity in its extracellular domain. It has been described that DPPIV inhibition delays the onset of type 1 diabetes and reverses the disease in non-obese diabetic (NOD) mice. The aim of the present study was to assess the effect of MK626, a DPPIV inhibitor, in type 1 diabetes incidence and in T lymphocyte subsets at central and peripheral compartments. Pre-diabetic NOD mice were treated with MK626. Diabetes incidence, insulitis score, and phenotyping of T lymphocytes in the thymus, spleen and pancreatic lymph nodes were determined after 4 and 6 weeks of treatment, as well as alterations in the expression of genes encoding ß-cell autoantigens in the islets. The effect of MK626 was also assessed in two in vitro assays to determine proliferative and immunosuppressive effects. Results show that MK626 treatment reduces type 1 diabetes incidence and after 6 weeks of treatment reduces insulitis. No differences were observed in the percentage of T lymphocyte subsets from central and peripheral compartments between treated and control mice. MK626 increased the expression of CD26 in CD8+ T effector memory (TEM) from spleen and pancreatic lymph nodes and in CD8+ T cells from islet infiltration. CD8+TEM cells showed an increased proliferation rate and cytokine secretion in the presence of MK626. Moreover, the combination of CD8+ TEM cells and MK626 induces an immunosuppressive response. In conclusion, treatment with the DPPIV inhibitor MK626 prevents experimental type 1 diabetes in association to increase expression of CD26 in the CD8+ TEM lymphocyte subset. In vitro assays suggest an immunoregulatory role of CD8+ TEM cells that may be involved in the protection against autoimmunity to ß pancreatic islets associated to DPPIV inhibitor treatment.


Assuntos
Linfócitos T CD8-Positivos/efeitos dos fármacos , Diabetes Mellitus Tipo 1/prevenção & controle , Dipeptidil Peptidase 4/efeitos dos fármacos , Inibidores da Dipeptidil Peptidase IV/farmacologia , Fosfato de Sitagliptina/análogos & derivados , Animais , Autoantígenos/genética , Linfócitos T CD8-Positivos/imunologia , Diabetes Mellitus Tipo 1/imunologia , Ilhotas Pancreáticas/efeitos dos fármacos , Ilhotas Pancreáticas/imunologia , Ativação Linfocitária , Camundongos , Camundongos Endogâmicos NOD , Fosfato de Sitagliptina/farmacologia , Fator de Crescimento Transformador beta/sangue
3.
Sensors (Basel) ; 13(11): 14367-97, 2013 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-24284770

RESUMO

The present work presents an improved method to align the measurement scale mark in an immersion hydrometer calibration system of CENAM, the National Metrology Institute (NMI) of Mexico, The proposed method uses a vision system to align the scale mark of the hydrometer to the surface of the liquid where it is immersed by implementing image processing algorithms. This approach reduces the variability in the apparent mass determination during the hydrostatic weighing in the calibration process, therefore decreasing the relative uncertainty of calibration.

4.
Lima; s.n; 2013. 73 p. tab, graf.
Tese em Espanhol | PERNAL | ID: pnc-15468

RESUMO

Antecedentes: La morbilidad asociada a la ruptura prematura de membranas puede ser mayor en neonatos pretérmino. Objetivo: Determinar la morbilidad materno-perinatal de la Rotura Prematura de Membranas Pretérmino en el Hospital Nacional Dos de Mayo en el periodo 2011-2012. Métodos: Revisión documentaria de las historias clínicas de las madres y neonatos prematuros que cumplieron criterios de selección, encontrando 170 casos en el periodo de estudio. Se muestran los resultados mediante estadística descriptiva. Resultados: La edad de las madres de neonatos prematuros con RPM fue 25,52 años. La forma de terminación del parto en 34,12 por ciento fue la vía vaginal, y en 65,88 por ciento por cesárea. El tiempo de latencia desde la RPM al parto en 9,41 por ciento fue dentro de las 6 primeras horas, en 21,18 por ciento dentro de las primeras 12 horas, y en 69,41 por ciento luego de las 24horas. Se emplearon corticoides para maduración pulmonar en 46,47 por ciento de casos, todos ellos con dexametasona, y en 27,65 por ciento se completó cuatro cursos. El 47,65 por ciento de neonatos fueron varones y 52,35 por ciento mujeres. En 82,35 por ciento de casos el líquido amniótico fue claro, 15,88 por ciento fue verde claro y en 1,76 por ciento fue purulento. No se usó antibióticos en 10 por ciento de casos, se usó sólo un antibiótico en 48,24 por ciento, dos antibióticos en 24,71 por ciento y tres antibióticos en 17,06 por ciento; el antibiótico más empleado fue la cefazolina. En 45,29 por ciento de madres se presentó endometritis; la duración de la hospitalización en las madres fue 5,73 días. El 14,71 por ciento de neonatos pretérmino fue llevado a alojamiento conjunto con la madre, 29,41 por ciento llegó a cuidados intermedios y 55,88 por ciento pasó a UCI; la indicación de hospitalización en UCI fue la sepsis en 55,79 por ciento, insuficiencia respiratoria en 20 por ciento, prematuridad extrema en 12,63 por ciento. Un 22,35 por ciento de neonatos no requirieron de apoyo...(AU)


Background: The morbidity associated with premature rupture of membranes may be higher in preterm infants. Objective: To determine maternal and perinatal morbidity of Premature Rupture of Membranes at the National Hospital Dos de Mayo in the period 2011-2012. Methods: Review of medical records documentary of mothers and preterm infants who met selection criteria, finding 170 cases in the study period. Results are shown using descriptive statistics. Results: The age of the mothers of preterm infants with RPM was 25.52 years. The manner of termination of labor was vaginal in 34.12 per cent, and by cesarean in 65.88 per cent. The latency from the RPM to birth was 9.41 per cent within the first 6 hours, in 21.18 per cent within the first 12 hours, and 69.41 per cent after 24 hours. Corticosteroids were used for 11Ing maturation in 46.47 per cent of cases, all with dexamethasone, and 27.65 per cent completed four courses. The 47.65 per cent of infants were male and 52.35 per cent female. In 82.35 per cent of cases the amniotic fluid was clear, light green in 15.88 per cent and in 1.76 per cent was purulent. No antibiotics were used in 10 per cent of cases, only one antibiotic was used in 48.24 per cent, two antibiotics in 24.71 per cent and three in 17.06 per cent, the most used antibiotic was cefazolin. In 45.29 per cent of mothers showed endometritis, the duration of hospitalization in mothers was 5.73 days. The 14.71 per cent of preterm infants was admitted with mother, intermediate care in 29.41 per cent and 55.88 per cent went to ICU, the indication for hospitalization in ICU was in 55.79 per cent sepsis, respiratory failure in 20 per cent, and in 12.63 per cent extreme prematurity. A 22.35 per cent of neonates did not required ventilatory support, 39.41 per cent required mechanical ventilation and 38.24 per cent used CPAP. In only 5.29 per cent of children there were no complications, a 91.18 per cent of cases had metabolic disorders, 90 per cent developed...(AU)


Assuntos
Humanos , Feminino , Gravidez , Adolescente , Adulto Jovem , Adulto , Ruptura Prematura de Membranas Fetais , Trabalho de Parto Prematuro , Doenças do Recém-Nascido , Morbidade , Mortalidade Infantil , Estudo Observacional , Estudos Retrospectivos , Estudos Transversais
5.
Lima; s.n; 2013. 73 p. tab, graf.
Tese em Espanhol | LILACS | ID: lil-713929

RESUMO

Antecedentes: La morbilidad asociada a la ruptura prematura de membranas puede ser mayor en neonatos pretérmino. Objetivo: Determinar la morbilidad materno-perinatal de la Rotura Prematura de Membranas Pretérmino en el Hospital Nacional Dos de Mayo en el periodo 2011-2012. Métodos: Revisión documentaria de las historias clínicas de las madres y neonatos prematuros que cumplieron criterios de selección, encontrando 170 casos en el periodo de estudio. Se muestran los resultados mediante estadística descriptiva. Resultados: La edad de las madres de neonatos prematuros con RPM fue 25,52 años. La forma de terminación del parto en 34,12 por ciento fue la vía vaginal, y en 65,88 por ciento por cesárea. El tiempo de latencia desde la RPM al parto en 9,41 por ciento fue dentro de las 6 primeras horas, en 21,18 por ciento dentro de las primeras 12 horas, y en 69,41 por ciento luego de las 24horas. Se emplearon corticoides para maduración pulmonar en 46,47 por ciento de casos, todos ellos con dexametasona, y en 27,65 por ciento se completó cuatro cursos. El 47,65 por ciento de neonatos fueron varones y 52,35 por ciento mujeres. En 82,35 por ciento de casos el líquido amniótico fue claro, 15,88 por ciento fue verde claro y en 1,76 por ciento fue purulento. No se usó antibióticos en 10 por ciento de casos, se usó sólo un antibiótico en 48,24 por ciento, dos antibióticos en 24,71 por ciento y tres antibióticos en 17,06 por ciento; el antibiótico más empleado fue la cefazolina. En 45,29 por ciento de madres se presentó endometritis; la duración de la hospitalización en las madres fue 5,73 días. El 14,71 por ciento de neonatos pretérmino fue llevado a alojamiento conjunto con la madre, 29,41 por ciento llegó a cuidados intermedios y 55,88 por ciento pasó a UCI; la indicación de hospitalización en UCI fue la sepsis en 55,79 por ciento, insuficiencia respiratoria en 20 por ciento, prematuridad extrema en 12,63 por ciento. Un 22,35 por ciento de neonatos no requirieron de apoyo...


Background: The morbidity associated with premature rupture of membranes may be higher in preterm infants. Objective: To determine maternal and perinatal morbidity of Premature Rupture of Membranes at the National Hospital Dos de Mayo in the period 2011-2012. Methods: Review of medical records documentary of mothers and preterm infants who met selection criteria, finding 170 cases in the study period. Results are shown using descriptive statistics. Results: The age of the mothers of preterm infants with RPM was 25.52 years. The manner of termination of labor was vaginal in 34.12 per cent, and by cesarean in 65.88 per cent. The latency from the RPM to birth was 9.41 per cent within the first 6 hours, in 21.18 per cent within the first 12 hours, and 69.41 per cent after 24 hours. Corticosteroids were used for 11Ing maturation in 46.47 per cent of cases, all with dexamethasone, and 27.65 per cent completed four courses. The 47.65 per cent of infants were male and 52.35 per cent female. In 82.35 per cent of cases the amniotic fluid was clear, light green in 15.88 per cent and in 1.76 per cent was purulent. No antibiotics were used in 10 per cent of cases, only one antibiotic was used in 48.24 per cent, two antibiotics in 24.71 per cent and three in 17.06 per cent, the most used antibiotic was cefazolin. In 45.29 per cent of mothers showed endometritis, the duration of hospitalization in mothers was 5.73 days. The 14.71 per cent of preterm infants was admitted with mother, intermediate care in 29.41 per cent and 55.88 per cent went to ICU, the indication for hospitalization in ICU was in 55.79 per cent sepsis, respiratory failure in 20 per cent, and in 12.63 per cent extreme prematurity. A 22.35 per cent of neonates did not required ventilatory support, 39.41 per cent required mechanical ventilation and 38.24 per cent used CPAP. In only 5.29 per cent of children there were no complications, a 91.18 per cent of cases had metabolic disorders, 90 per cent developed...


Assuntos
Humanos , Feminino , Gravidez , Adolescente , Adulto Jovem , Ruptura Prematura de Membranas Fetais , Mortalidade Infantil , Doenças do Recém-Nascido , Morbidade , Trabalho de Parto Prematuro , Estudos Transversais , Estudo Observacional , Estudos Retrospectivos
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