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1.
Rev. enferm. UFPE on line ; 15(1): [1-6], jan. 2021.
Artigo em Português | BDENF - Enfermagem | ID: biblio-1147004

RESUMO

Objetivo: conhecer o gerenciamento de resíduos de serviços de saúde oriundos de materiais hospitalares e medicamentos descartados devido à data de validade expirada, em um hospital universitário. Método: trata-se de um estudo quantitativo, descritivo, transversal, a ser realizado em um hospital universitário. Coletar-se-ão os dados por meio de consulta ao banco de dados e formulários restritos do cenário de estudo. Investigar-se-ão as seguintes variáveis: descrição do resíduo; período de destinação final; custo do produto e da destinação final; quantidade e local de geração. Organizar-se-ão os dados em planilhas de Excel, analisando-os pelo software Statistical Package for the Social Sciences, versão 19. Realizar-se-á o estudo após a aprovação dos Comitês de Ética responsáveis. Resultados esperados: pretendem-se obter informações que subsidiem a intervenção por meio de planejamento, monitoramento e avaliação logística de todos os processos da gestão de resíduos de serviços de saúde.(AU)


Assuntos
Gerenciamento de Resíduos , Estabilidade de Medicamentos , Equipamentos e Provisões Hospitalares , Resíduos de Serviços de Saúde , Epidemiologia Descritiva , Estudos Transversais , Hospitais Universitários
3.
Acta Cir Bras ; 35(7): e202000703, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32813773

RESUMO

Purpose To analyze changes in the thermal pattern in the skin graft receptor bed, after the use of therapeutic ultrasound through the thermographic images. Methods Eighteen Rattus norvegicus albinus Wistar, separated into two groups: GST groups (without tumor and without treatment with ultrasound) and GT (with tumor and treatment with ultrasound). In the GT group, induction of carcinogenesis was performed by single intradermal application of 0.05 ml DMBA at 0.5%, diluted in acetone. Subsequently, a technique of reconstructive grafting surgery of the mesh type was performed in both groups and treatment with therapeutic ultrasound was performed in the GT group the alternate day protocol at 3, 6, 10 and 15 days after the procedure. The thermographic evaluation occurred on days 3, 6, 10 and 15 after the grafting. Results There was a significant difference between the statistical evaluation of the temperature of the control group when compared to the treated group, on the different evaluation days (p <0.0001). Conclusion The thermographic analysis of the images was effective in evaluating the healing process, being the use of thermography feasible to evaluate changes in the thermal standard in the surgical bed, besides the beneficial effects of the US.


Assuntos
Termografia , Terapia por Ultrassom , Animais , Ratos , Ratos Wistar , Transplante de Pele , Cicatrização
4.
Pharmacol Biochem Behav ; 189: 172841, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31893526

RESUMO

Environmental pollution caused by antipsychotic residues is a relevant ecological problem. Studies revealed that residues of these drugs are present in a wide range of different ecosystems and can have adverse effects on non-target organisms even in low environmental concentrations. Among these antipsychotic drugs, aripiprazole (APPZ) is a second-generation atypical antipsychotic that is a partial agonist of dopaminergic and serotoninergic receptors. APPZ is used to treat schizophrenia, bipolar disorder, autism, obsessive-compulsive disorder, and anxiety or panic disorders. Thus, in this study we posed the following question: "What will be the behavioral effects of waterborne APPZ on fish?" To answer this question, we exposed adult zebrafish to different APPZ concentrations (0.556, 5.56, and 556 ng/L) for 15 min and evaluated their exploratory, anxiety-like, social, and anti-predatory behaviors. Our results showed that, despite the apparent beneficial reversal of stress-induced social impairment and anxiety-like behavior, APPZ exposure impaired the anti-predatory reaction of adult zebrafish. Taken altogether, our results show that APPZ-exposed zebrafish may have a decreased perception of predators, even at concentrations lower than those already detected in the environment. A failure to exhibit an antipredatory response may favor the predator, decrease the fitness of the prey species, and, consequently, affect the food chain. Our results highlight the risks and consequences associated with APPZ residues in water, which may affect aquatic life and endanger species that depend on appropriate behavioral responses for survival.

5.
Acta cir. bras ; 35(7): e202000703, 2020. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1130663

RESUMO

Abstract Purpose To analyze changes in the thermal pattern in the skin graft receptor bed, after the use of therapeutic ultrasound through the thermographic images. Methods Eighteen Rattus norvegicus albinus Wistar, separated into two groups: GST groups (without tumor and without treatment with ultrasound) and GT (with tumor and treatment with ultrasound). In the GT group, induction of carcinogenesis was performed by single intradermal application of 0.05 ml DMBA at 0.5%, diluted in acetone. Subsequently, a technique of reconstructive grafting surgery of the mesh type was performed in both groups and treatment with therapeutic ultrasound was performed in the GT group the alternate day protocol at 3, 6, 10 and 15 days after the procedure. The thermographic evaluation occurred on days 3, 6, 10 and 15 after the grafting. Results There was a significant difference between the statistical evaluation of the temperature of the control group when compared to the treated group, on the different evaluation days (p <0.0001). Conclusion The thermographic analysis of the images was effective in evaluating the healing process, being the use of thermography feasible to evaluate changes in the thermal standard in the surgical bed, besides the beneficial effects of the US.

6.
Indian J Dent Res ; 30(3): 424-427, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31397420

RESUMO

Context: Zinc-oxide eugenol (ZOE) is frequently used due to its satisfactory biological response, sedative effect on the pulp, and easy removal. However, literature is very controversial about the influence of the temporary cement-containing eugenol on the bond strength properties. Aims: This study aims to clarify the literature controversy by evaluating the bond strength of ZOE or ZOE-free applied before bonding procedures and the 7-day resting period after the first session. Settings and Designs: Twelve recently extracted third molars were randomly divided into three groups: Group 1: Control (without treatment), Group 2: Temp-Bond NE; Group 3: Temp-Bond. Subjects and Methods: After temporary restorations, the teeth were immersed in distilled water and stored for 7 days at 37°C. The temporary cement was mechanically removed, and prophylaxis was performed. The adhesive procedures were performed, and a 6-mm-high composite resin block was built. The teeth were immediately stored at 100% relative humidity at 37°C for 24 hours. Specimens with 0.7 (±0.2) mm2 of cross-sectional area were obtained and subjected to a microtensile bond strength (µTBS) test at 1 mm/min until failure. Statistical Analysis Used: Analysis of variance (ANOVA) and Tukey's post hoc test were used for statistical analysis. Results: ZOE or ZOE-free cement did not interfere in the µTBS between resin composite and dentine when used with a two-step-etch-rinse adhesive material, considering 7 days of resting period after the first session, by the ANOVA and Tukey's post hoc test (P < 0.05). Conclusions: The authors recommend the removal of the temporary cementation at least seven days after the first session and suggest that the clinicians follow-up further studies to use the cement in case its removal is recommended within a longer period.


Assuntos
Colagem Dentária , Cimento de Óxido de Zinco e Eugenol , Resinas Compostas , Cimentos Dentários , Restauração Dentária Temporária , Dentina , Adesivos Dentinários , Eugenol , Teste de Materiais , Cimentos de Resina , Resistência à Tração , Zinco
7.
J Virol Methods ; 274: 113714, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31412271

RESUMO

Hepatitis C pseudoparticles (HCVpp) are used to evaluate HCV cell entry while screening for neutralizing antibodies induced upon vaccination or while screening for new antiviral drugs. In this work we explore the stable production of HCVpp aiming to reduce the variability associated with transient productions. The performance of stably produced HCVpp was assessed by evaluating the influence of Human Serum and the impact of CD81 cellular expression on the infectivity of HCVpp. After evaluating the performance of stably produced HCVpp we studied the effect of co-expressing p7NS2 openreading frame (ORF) on HCVpp infectivity. Our data clearly shows an enhanced infectivity of HCVppp7NS2. Even though the exact mechanism was not completely elucidated, the enhanced infectivity of HCVppp7NS2 is neither a result of an increase production of virus particles nor a result from increased envelope density. The inhibitory effect of p7 inhibitory molecules such as rimantadine suggests a direct contribution of p7 ion channel for the enhanced infectivity of HCVppp7NS2 which is coherent with a pH-dependent cell entry mechanism. In conclusion, we report the establishment of a stable production system of HCVpp with enhanced infectivity through the overexpression of p7NS2 ORF contributing to improve HCV entry assessment assays widely used in antiviral drug discovery and vaccine development.


Assuntos
Expressão Gênica , Hepacivirus/fisiologia , Vírus da Leucemia Murina/crescimento & desenvolvimento , Proteínas não Estruturais Virais/biossíntese , Proteínas Virais/biossíntese , Cultura de Vírus/métodos , Internalização do Vírus , Linhagem Celular , Genes Reporter , Vetores Genéticos , Proteínas de Fluorescência Verde/análise , Proteínas de Fluorescência Verde/genética , Hepacivirus/genética , Humanos , Vírus da Leucemia Murina/genética , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/genética , Coloração e Rotulagem/métodos , Proteínas não Estruturais Virais/genética , Proteínas Virais/genética
8.
mSphere ; 4(3)2019 06 19.
Artigo em Inglês | MEDLINE | ID: mdl-31217305

RESUMO

Aspergillus fumigatus is a filamentous fungus which causes invasive pulmonary aspergillosis in immunocompromised individuals. In fungi, cell signaling and cell wall plasticity are crucial for maintaining physiologic processes. In this context, Msb2 is an important signaling mucin responsible for activation of a variety of mitogen-activated protein kinase (MAPK)-dependent signaling pathways that regulate cell growth in several organisms, such as the cell wall integrity (CWI) pathway. Here, we aimed to characterize the MSB2 homologue in A. fumigatus Our results showed that MsbA plays a role in the vegetative and reproductive development of the fungus, in stress adaptation, and in resistance to antifungal drugs by modulating the CWI pathway gene expression. Importantly, cell wall composition is also responsible for activation of diverse receptors of the host immune system, thus leading to a proper immune response. In a model of acute Aspergillus pulmonary infection, results demonstrate that the ΔmsbA mutant strain induced less inflammation with diminished cell influx into the lungs and lower cytokine production, culminating in increased lethality rate. These results characterize for the first time the role of the signaling mucin MsbA in the pathogen A. fumigatus, as a core sensor for cell wall morphogenesis and an important regulator of virulence.IMPORTANCE Aspergillus fumigatus is an opportunistic fungus with great medical importance. During infection, Aspergillus grows, forming hyphae that colonize the lung tissue and invade and spread over the mammal host, resulting in high mortality rates. The knowledge of the mechanisms responsible for regulation of fungal growth and virulence comprises an important point to better understand fungal physiology and host-pathogen interactions. Msb2 is a mucin that acts as a sensor and an upstream regulator of the MAPK pathway responsible for fungal development in Candida albicans and Aspergillus nidulans Here, we show the role of the signaling mucin MsbA in the pathogen A. fumigatus, as a core sensor for cell wall morphogenesis, fungal growth, and virulence. Moreover, we show that cell wall composition, controlled by MsbA, is detrimental for fungal recognition and clearance by immune cells. Our findings are important for the understanding of how fungal sensors modulate cell physiology.


Assuntos
Transportadores de Cassetes de Ligação de ATP/genética , Aspergillus fumigatus/genética , Proteínas de Bactérias/genética , Parede Celular/metabolismo , Regulação Fúngica da Expressão Gênica , Mucinas/genética , Transportadores de Cassetes de Ligação de ATP/imunologia , Animais , Aspergilose/imunologia , Aspergillus fumigatus/imunologia , Proteínas de Bactérias/imunologia , Feminino , Proteínas Fúngicas/genética , Proteínas Fúngicas/imunologia , Peptídeos e Proteínas de Sinalização Intracelular/genética , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Mucinas/imunologia , Transdução de Sinais , Virulência
9.
J Conserv Dent ; 22(5): 436-440, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-33082658

RESUMO

Aims: The aim of this study is to evaluate in vitro the effect of application of bromelain enzyme on the microleakage of composite resin restorations after external tooth bleaching using spectrophotometric evaluation. Subjects and Methods: Buccal Class V cavities were prepared on the surface of fifty intact premolars, which were randomly divided into five groups. All cavities were filled with composite resin. Group I: Teeth were not bleached but restored (n = 10). External bleaching with 35% hydrogen peroxide was carried out for the rest of the specimensGroup II: Cavities were restored immediately after bleaching (n = 10)Group III: Cavities were restored after a delay of 3 weeks (n = 10)Group IV: Cavities were treated with sodium ascorbate after bleaching and then restored (n = 10)Group V: Cavities were treated with bromelain enzyme solution after bleaching and then restored (n = 10). Microleakage was assessed by the dye extraction method using a spectrophotometer. Statistical Analysis Used: The data were analyzed statistically by comparison of mean microleakage and post hoc test using SPSS 2.0 software. Results: Group I displayed the least amount of microleakage, whereas Group II showed the greatest amount of microleakage (P < 0.05). Groups III, IV, and V showed a significantly lower amount of microleakage compared to Group II (P < 0.05). There was no statistically significant difference between Groups IV and V. Conclusions: Microleakage increased significantly after external bleaching with 35% hydrogen peroxide, and decreased when the bleached teeth were treated with antioxidants. Ten percent bromelain enzyme was effective in decreasing microleakage; however, its efficacy was similar to 10% sodium ascorbate.

10.
Rev. enferm. UFPE on line ; 13: [1-5], 2019.
Artigo em Português | BDENF - Enfermagem | ID: biblio-1095245

RESUMO

Objetivo: descrever como acontece a comunicação verbal oral e escrita entre os gestores e as equipes das Unidades de Pronto Atendimento (UPAs 24h). Método: trata-se-de estudo qualitativo, descritivo. Utilizar-se-á o estudo de caso como método, tendo como unidade de análise as quatro UPAs 24h de um município e os participantes da pesquisa serão os profissionais que ocupam função de gestão nas UPAs 24h, entre eles, gerentes, referências técnicas médicas e de Enfermagem, amparado nos critérios de saturação dos dados. Realizar-se-á a coleta de dados por meio de entrevista semiestruturada utilizando um roteiro validado. Analisar-se-á o conteúdo das entrevistas por meio da técnica de Análise de Conteúdo. Resultados esperados: pretende-se possibilitar a elaboração de um instrumento norteador, a partir das tecnologias gerenciais em saúde, para o alcance da comunicação efetiva entre gestores e equipes das UPAs 24h.(AU)


Objective: to describe how oral and written verbal communication happens between managers and teams of Emergency Care Units (24-hour ECUs). Method: this is a qualitative, descriptive study. The case study will be used as a method, having as a unit of analysis the four 24h ECUs of a municipality and the research participants will be the professionals who perform the management function in the 24h ECUs, including managers, medical and technical references based on data saturation criteria. Data collection will be performed through semi-structured interviews using a validated script. The content of the interviews will be analyzed using the Content Analysis technique. Expected results: it is intended to enable the development of a guiding instrument, based on health management technologies, to achieve effective communication between managers and teams of 24-hour ECUs.(AU)


Objetivo: describir cómo ocurre la comunicación verbal oral y escrita entre los gerentes y los equipos de las Unidades de Atención de Emergencia (UPAs 24h). Método: este es un estudio cualitativo y descriptivo. Se utilizará el estudio de caso como método, tomando como unidad de análisis las cuatro UPAs de 24 horas de un municipio y los participantes en la investigación serán los profesionales que ocupan la función de gestión en las UPAs de 24 horas, incluidos los gerentes, las referencias técnicas médicas y de Enfermería, respaldadas por los criterios de saturación de datos. La recopilación de datos se realizará a través de entrevistas semiestructuradas utilizando un guión validado. El contenido de las entrevistas se analizará utilizando la técnica de Análisis de Contenido. Resultados esperados: está destinado a permitir la elaboración de un instrumento de guía, basado en tecnologías de gestión de la salud, con el fin de lograr una comunicación efectiva entre los gerentes y equipos de las UPAs 24 horas(AU)


Assuntos
Humanos , Masculino , Feminino , Equipe de Assistência ao Paciente , Comunicação , Serviços Médicos de Emergência , Gestor de Saúde , Assistência Ambulatorial , Relações Interprofissionais , Epidemiologia Descritiva , Pesquisa Qualitativa
11.
J Dent ; 77: 66-71, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30009857

RESUMO

OBJECTIVES: To evaluate the antibacterial effects, cytotoxicity and microtensile bond strength of an adhesive containing low concentrations of silver nanoparticles (NAg). METHODS: Various concentrations of NAg (50, 100, 150, 200 and 250 ppm) were incorporated into the primer of the Scotchbond Multi-Purpose adhesive system (SBMP). Antibacterial activity was examined using a broth microdilution assay to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), agar diffusion assay and the MTT assay was used to examine the biofilm metabolic activity (S. mutans). The Microtensile Bond Test (µTBS) was performed after 24 h, followed by 6-months storage in distilled water. Cytotoxicity was assessed with an MTT reduction assay in human dental pulp stem cells viability after exposure to Nag-conditioned culture media during 0, 24, 48, and 72 h. The results were statistically analyzed (α ≤ 0.05). RESULTS: MIC was found between NAg 25 and 50 ppm MBC was determined at 50 ppm of NAg. Bacterial activity inhibition was higher than control in all NAg groups compared to control in agar diffusion assay. Biofilm inhibition was statistically higher in 250 ppm NAg than control. All NAg groups and SBMP presented similar cytotoxicity in each period. Adhesives with NAg 200 and 250 ppm and SBMP (control) presented the highest µTBS values, similar to that of SBMP control, in both instances (24 h and 6 months) (p > 0.05). CONCLUSIONS: The commercial primer containing NAg 250 ppm showed both antibacterial effect and reliable bond strength with no cytotoxicity increase. The addition of NAg to primers seems promising for the improvement of conventional dental adhesives efficacy. CLINICAL SIGNIFICANCE: The addition of low concentrations of NAg (250 ppm) to primers were effective to improve antibacterial effect preserving the bond strength and the biocompatibility of the commercial product. NAg/primer association could protect the tooth-adhesive interface increasing dental restoration longevity.


Assuntos
Antibacterianos/toxicidade , Colagem Dentária , Cimentos Dentários , Nanopartículas Metálicas/toxicidade , Prata , Biofilmes , Humanos , Teste de Materiais , Cimentos de Resina , Resistência à Tração
12.
Ticks Tick Borne Dis ; 9(2): 245-253, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-28941935

RESUMO

Piroplasmoses are one of the most prevalent arthropod-borne diseases of animals. The present work aimed to investigate the occurrence of piroplasmid in wild mammals, domestic dogs and ectoparasites in southern Pantanal region, central-western Brazil. For that purpose, blood or tissue samples from 31 Nasua nasua, 78 Cerdocyon thous, 7 Leopardus pardalis, 42 dogs, 110 wild rodents, and 30 marsupials, and 1582 ticks were submitted to PCR assays for piroplasmid targeting 18SrRNA and hps70 genes. Seven dogs, one C. thous, five L. pardalis, three N. nasua, six wild rodents, eight Amblyomma parvum, two Amblyomma sculptum and one Amblyomma ovale were positive for piroplasmid-PCR assays. Genotypes closely related to Babesia vogeli were detected in six dogs and five wild rodents. While genotypes closely related to Babesia caballi were detected in one C. thous, one dog, one A. ovale and one A. sculptum, genotypes closely related to Babesia bigemina and Babesia bovis were detected in four A. parvum ticks. Four sequences obtained from A. parvum, three coatis and one wild rodent were closely related to Theileria equi. Cytauxzoon spp. was detected in four ocelots. The present study revealed that wild and domestic animals in Brazilian southern Pantanal are exposed to different piroplasmid species.


Assuntos
Animais Selvagens , Doenças do Cão/epidemiologia , Ixodidae/parasitologia , Piroplasmida/classificação , Infecções Protozoárias em Animais/epidemiologia , Sifonápteros/parasitologia , Animais , Biodiversidade , Brasil/epidemiologia , Doenças do Cão/parasitologia , Cães , Proteínas de Choque Térmico HSP70/análise , Filogenia , Prevalência , Infecções Protozoárias em Animais/parasitologia , Proteínas de Protozoários/análise , RNA de Protozoário/análise , RNA Ribossômico 18S/análise , Análise de Sequência de DNA , Estudos Soroepidemiológicos
13.
J Conserv Dent ; 20(3): 214-218, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29279629

RESUMO

Invasive cervical resorption (ICR) is an aggressive and invasive form of external tooth resorption that commences in the cervical region which can be managed using either a nonsurgical, surgical or a combination of nonsurgical and surgical approach. The restoration of resorptive defects having a coronal and radicular extent can be challenging as a single material cannot be used to restore the entire defect. This case presented with Heithersay's Class 3 ICR lesion in tooth #12, which was managed initially using a surgical approach and restoration of ICR defect with a combination of resin modified glass ionomer cement, composite resin and mineral trioxide aggregate (MTA). However, the patient presented with pulpal symptoms 2 months later which warranted a root canal therapy. An internal approach was then used to debride and remove remnant fibro-osseous tissue. The defect was then repaired with MTA. A 1 year follow-up demonstrated adequate periapical healing and no pathologic changes around the restored resorptive defect.

14.
J Conserv Dent ; 20(2): 68-71, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28855749

RESUMO

BACKGROUND: Most often used intracanal medicament is calcium hydroxide (Ca(OH)2). Removal of Ca(OH)2 before obturation is mandatory. Different irrigation solutions and devices were tested in the previous study for this purpose. AIM: The aim of this study was to evaluate the comparative efficacy of different irrigation techniques in removing intracanal medicament using cone-beam computed tomography (CBCT). MATERIALS AND METHODS: Cleaning and shaping was carried out in 42 freshly extracted single-rooted mandibular first premolars using Protaper Rotary Files. Intracanal medicament (silicone oil-based Ca(OH)2 paste) was placed and left for 2 weeks in the root canal. Medicament was then removed by five different irrigation techniques: EndoActivator, EndoVac, ultrasonic irrigation with Endo-U-File, F-file, and Max-i-Probe needle. Changes in volume percentage were measured using CBCT and results were statistically analyzed using Levene's statistics and ANOVA. RESULTS: Remnants of Ca(OH)2 were found in all experimental groups. No significant difference was detected among the groups. F-file showed maximum removal efficacy of approximately 48%. CONCLUSION: (1) There is no statistically significant difference between any irrigation technique for removal of oil-based Ca(OH)2. (2) F-file demonstrated better results among all the other systems.

15.
J Clin Diagn Res ; 11(4): ZC136-ZC141, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28571281

RESUMO

INTRODUCTION: Decades of research has helped to increase our knowledge of dental caries and reduce its prevalence. However, according to World Oral Health report, dental caries still remains a major dental disease. Fluoride therapy has been utilised in a big way to halt caries progression, but has been met with limitations. This has paved the way for the development of newer preventive agents that can function as an adjunct to fluoride or independent of it. AIM: The purpose of the present study was to evaluate the ability of Casein Phosphopeptide-Amorphous Calcium Phosphate Fluoride (CPP ACPF), Bioactive Glass (BAG), fluoride enhanced Hydroxyapatite (HA) gel and self-assembling peptide P11-4 to remineralise artificial carious lesions in enamel in vitro using a 30 day pH cycling model through surface microhardness analysis and SEM. MATERIALS AND METHODS: Sixty enamel samples were divided into five groups of 12 samples each. The control Group A consisted of intact enamel samples, Group B: CPP-ACPF (Tooth Mousse Plus), Group C: BAG (SHY- NM), Group D: fluoride enhanced HA gel (ReminPro) and Group E: Self-assembling peptide P11-4 (Curodont Protect). All groups excluding the control group were subjected to demineralisation following which four of these groups were remineralised using the four remineralising agents. The treated groups were subjected to pH cycling over a period of 30 days. This was followed by assessment of surface microhardness and SEM for qualitative evaluation of surface changes. The results were analysed by One-Way Analysis Of Variance (ANOVA). Multiple comparisons between groups were performed by paired t-test and post-hoc Tukey test. RESULTS: The results of the study revealed that remineralisation of enamel was the highest in samples of Group E (Self assembling peptide P11-4) followed by Group B (CPP-ACPF), Group C (BAG) and Group D (fluoride enhanced HA gel). There was a significant difference (p<0.05) in the remineralising ability between the self assembling peptide P11-4 group and BAG and fluoride enhanced HA gel group. Although no significant difference was observed between the self assembling peptide P11-4 and CPP-ACPF group, the self assembling peptide P11-4 remineralised the enamel lesions more effectively. SEM photomicrographs of the test groups demonstrated either amorphous crystals or particles scattered on the surface or lines of remineralisation along the prismatic borders. CONCLUSION: Self assembling peptide P11-4 demonstrated promising results by effectively and significantly remineralising the enamel lesions as compared to other test agents.

16.
Vet Parasitol ; 237: 37-46, 2017 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-28291601

RESUMO

Hepatozoon parasites comprise intracellular apicomplexan parasites transmitted to vertebrate animals by ingestion of arthropods definitive hosts. The present work aimed to investigate the occurrence of Hepatozoon spp. in wild animals, domestic dogs and their respective ectoparasites, in southern Pantanal region, central-western Brazil, by molecular techniques. Between August 2013 and March 2015, 31 coatis (Nasua nasua), 78 crab-eating foxes (Cerdocyon thous), seven ocelots (Leopardus pardalis), 42 dogs (Canis lupus familiaris), 110 wild rodents (77 Thichomys fosteri, 25 Oecomys mamorae, and 8 Clyomys laticeps), 30 marsupials (14 Thylamys macrurus, 11 Gracilinanus agilis, 4 Monodelphis domestica and 1 Didelphis albiventris), and 1582 ticks and 80 fleas collected from the sampled animals were investigated. DNA samples were submitted to PCR assays for Hepatozoon spp. targeting 18S rRNA gene. Purified amplicons were directly sequenced and submitted to phylogenetic analysis. A high prevalence of Hepatozoon among carnivores (C. thous [91.02%], dogs [45.23%], N. nasua [41.9%] and L. pardalis [71.4%]) was found. However, ticks and fleas were negative to Hepatozoon PCR assays. By phylogenetic analysis based on 18S rRNA sequences, Hepatozoon sequences amplified from crab-eating foxes, dogs, coatis and ocelots clustered with sequences of H. canis, H. americanum and H. felis. The closely related positioning of Hepatozoon sequences amplified from wild rodents and T. macrurus marsupial to Hepatozoon from reptiles and amphibians suggest a possible transmission of those Hepatozoon species between hosts by ectoparasites or by predation. Hepatozoon haplotypes found circulating in wild rodents seem to present a higher degree of polymorphism when compared to those found in other groups of animals. Although rodents seem not to participate as source of Hepatozoon infection to wild carnivores and domestic dogs, they may play an important role in the transmission of Hepatozoon to reptiles and amphibians in Pantanal biome.


Assuntos
Coccidiose/veterinária , Doenças do Cão/epidemiologia , Eucoccidiida/isolamento & purificação , Sifonápteros/parasitologia , Carrapatos/parasitologia , Anfíbios , Animais , Brasil/epidemiologia , Coccidiose/epidemiologia , Coccidiose/parasitologia , Coccidiose/transmissão , Doenças do Cão/parasitologia , Doenças do Cão/transmissão , Cães , Eucoccidiida/classificação , Eucoccidiida/genética , Feminino , Geografia , Masculino , Mamíferos , Filogenia , Prevalência , Répteis , Roedores , Análise de Sequência de DNA/veterinária
17.
J Conserv Dent ; 20(5): 365-369, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29386788

RESUMO

Excessive tooth structure loss is a common iatrogenic error encountered during endodontic practice. Conservative treatment planning is essential to maintain the structural integrity in such teeth. This case report elucidates a novel approach in sculpting Biodentine as a dentin substitute followed by internal bleaching and restoration with fiber-reinforced composite.

18.
Vet. Parasitol. ; 237: 37-46, 2017.
Artigo | Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP | ID: but-ib15329

RESUMO

Hepatozoon parasites comprise intracellular apicomplexan parasites transmitted to vertebrate animals by ingestion of arthropods definitive hosts. The present work aimed to investigate the occurrence of Hepatozoon spp. in wild animals, domestic dogs and their respective ectoparasites, in southern Pantanal region, central-western Brazil, by molecular techniques. Between August 2013 and March 2015, 31 coatis (Nasua nasua), 78 crab-eating foxes (Cerdocyon thous), seven ocelots (Leopard us pardalis), 42 dogs (Canis lupus familiaris), 110 wild rodents (77 Thichomys fosteri, 25 Oecomys mamorae, and 8 Clyomys laticeps), 30 marsupials (14 Thylamys macrurus, 11 Gracilinanus agilis, 4 Monodelphis domestica and 1 Didelphis albiventris), and 1582 ticks and 80 fleas collected from the sampled animals were investigated. DNA samples were submitted to PCR assays for Hepatozoon spp. targeting 18S rRNA gene. Purified amplicons were directly sequenced and submitted to phylogenetic analysis. A high prevalence of Hepatozoon among carnivores (C. thous [91.02%], dogs [45.23%], N. nasua [41.9%] and L. pardalis [71.4%1) was found. However, ticks and fleas were negative to Hepatozoon PCR assays. By phylogenetic analysis based on 18S rRNA sequences, Hepatozoon sequences amplified from crab-eating foxes, dogs, coatis and ocelots clustered with sequences of H. canis, H. americanum and H. felis. The closely related positioning of Hepatozoon sequences amplified from wild rodents and T. macrurus marsupial to Hepatozoon from reptiles and amphibians suggest a possible transmission of those Hepatozoon species between hosts by ectoparasites or by predation. Hepatozoon haplotypes found circulating in wild rodents seem to present a higher degree of polymorphism when compared to those found in other groups of animals. Although rodents seem not to participate as source of Hepatozoon infection to wild carnivores and domestic dogs, they may play an important role in the transmission of Hepatozoon to reptiles and amphibians in Pantanal biome.

19.
Restor Dent Endod ; 41(2): 143-7, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-27200283

RESUMO

The reconstruction of structurally compromised posterior teeth is a rather challenging procedure. The tendency of endodontically treated teeth (ETT) to fracture is considerably higher than vital teeth. Although posts and core build-ups followed by conventional crowns have been generally employed for the purpose of reconstruction, this procedure entails sacrificing a considerable amount of residual sound enamel and dentin. This has drawn the attention of researchers to fibre reinforcement. Fibre-reinforced composite (FRC), designed to replace dentin, enables the biomimetic restoration of teeth. Besides improving the strength of the restoration, the incorporation of glass fibres into composite resins leads to favorable fracture patterns because the fibre layer acts as a stress breaker and stops crack propagation. The following case report presents a technique for reinforcing a badly broken-down ETT with biomimetic materials and FRC. The proper utilization of FRC in structurally compromised teeth can be considered to be an economical and practical measure that may obviate the use of extensive prosthetic treatment.

20.
J Clin Diagn Res ; 10(11): ZD13-ZD15, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28050515

RESUMO

This case report describes a novel technique of restoring endodontically treated teeth. Hidden caries causing irreversible pulpitis in a mandibular molar with Radix Entomolaris (RE) was evident. The intact occlusal surface anatomy was duplicated before preparing an access cavity to replicate the original occlusal surface in the post endodontic restoration using (Computer-Aided Design and Computer-Aided Manufacturing) CAD/CAM technique. This report highlights uniquely designed onlay utilizing the benefits of contemporary materials and advanced technology.

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