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1.
Braz. j. biol ; 83: e244784, 2023. tab, graf
Artigo em Inglês | LILACS-Express | LILACS, VETINDEX | ID: biblio-1278541

RESUMO

Abstract The work aimed to evaluate the weight-length relationship and the condition factor, characterizing the biometry, anatomy, histology and volumetric density of the liver of Brycon amazonicus, in different stages of body growth. The experiment used twenty specimens in four stages of body growth (PI, PII, PIII and PIV) harvested every 90 days, containing five specimens, each group. The livers were dissected, weighed (g) and processed routinely using the hematoxylin and eosin technique. The data were submitted to analysis of variance, Pearson's correlation test and linear regression. The equation that represented the weight-length relation was W = 0.05902 x L2.63, with negative allometric growth, but with a relative condition factor equal to 1.0. The liver was divided into three lobes with the gallbladder close to the right lobe and its color varied from light red to dark red, not varying in relation to other fish species. The hepatosomatic relationship followed body growth until the PII group stage and then declined, demonstrating the behavior of its development in Brycon amazonicus. The organ consists predominantly of hepatocytes, followed by sinusoidal vessels and capillaries, with histological morphology similar to that of many species of fish. Melanomacrophage centers were found only in the most developed animals, but in small quantities, prompting the development of new research on this cell, in this species. In this way, research of this nature allows the characterization of fish species, helping to improve breeding methods, understanding pathological processes caused by diseases, and obtaining better productive capacity, serving an increasingly demanding and prosperous market.


Resumo O trabalho objetivou avaliar a relação peso-comprimento e o fator de condição, caracterizando a biometria, anatomia, histologia e densidade volumétrica do fígado de Brycon amazonicus, em diferentes estágios de crescimento corporal. O experimento utilizou vinte espécimes em quatro fases de crescimento corporal (PI, PII, PIII e PIV) colhidos a cada 90 dias, contendo cinco espécimes, cada grupo. Os fígados foram dissecados, pesados (g) e processados rotineiramente pela técnica da hematoxilina e eosina. Os dados foram submetidos à análise de variância, teste de correlação de Pearson e regressão linear. A equação que representou a relação peso-comprimento foi W = 0,05902 x L2,63, com crescimento alométrico negativo, mas com fator de condição relativo igual a 1,0. O fígado apresentou-se dividido em três lobos com a vesícula biliar próxima ao lobo direito e sua cor variou de vermelho claro a vermelho escuro, não variando em relação a outras espécies de peixes. A relação hepatossomática acompanhou o crescimento corporal até a fase do grupo PII e então declinou, demonstrando o comportamento de seu desenvolvimento em Brycon amazonicus. O órgão constitui-se predominantemente por hepatócitos, seguido de vasos e capilares sinusoidais, com a morfologia histológica semelhante ao de muitas espécies de peixes. Centros melanomacrófagos foram encontrados apenas nos animais mais desenvolvidos, mas em pequena quantidade, instigando o desenvolvimento de novas pesquisas sobre esta célula, nesta espécie. Desta forma, pesquisas desta natureza permitem a caracterização de espécies de peixes, auxiliando no aperfeiçoamento de métodos de criação, compreensão de processos patológicos provocados por enfermidades, e obtenção de melhor capacidade produtiva, atendendo um mercado cada vez mais exigente e próspero.

2.
Res Vet Sci ; 139: 87-93, 2021 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-34265511

RESUMO

Regional lymph node status impacts survival in dogs with malignant mammary tumors. However, few studies have evaluated extracapsular extension and tumor implants in regional lymph node metastases in dogs with mammary carcinoma. Therefore, 84 cases of mammary carcinomas with metastases in inguinal and/or axillary lymph nodes from female dogs of different breeds and a total of 139 metastatic lymph nodes were evaluated by routine histological staining. Clinical and pathological characteristics of primary tumors as well as the presence of extracapsular extension and tumor implants in the lymph nodes were analyzed, in addition to survival. One to 5 lymph nodes were evaluated in each case. Extracapsular extension and tumor implants were present in 17.9% and 39.3% of cases, respectively. The simultaneous presence of extracapsular extension and tumor implants were associated with an increased risk of death (hazard ratio 10.46). In addition, "special type carcinomas", high histological grade (grade III), and presence of extracapsular extension associated with tumor implants were related to a worse prognosis and shorter survival times (p < 0.05). Based on these results, we highlight the importance of identifying extracapsular extension and tumor implants in dogs with metastatic mammary carcinomas, as they are associated with a higher risk of death and shorter survival.

3.
Enferm. foco (Brasília) ; 12(1): 196-201, jun. 2021. ilus
Artigo em Português | LILACS, BDENF - Enfermagem | ID: biblio-1280852

RESUMO

Objetivo: Descrever o desenvolvimento de um protótipo de game educativo para prevenção de acidentes comuns na infância. Metodologia: Estudo descritivo sobre desenvolvimento tecnológico de um protótipo de game educativo, proveniente de pesquisa original sobre utilização da gamificação como ferramenta educativa para prevenção de acidentes na infância. O desenvolvimento do protótipo guiou-se pela estratégia Design Thinking, com uso do software educacional Scratch que é uma plataforma online, gratuita. Os cenários e personagens foram construídos com figuras disponíveis em acesso livre na internet. Resultados: O protótipo do game foi intitulado "Detetives do perigo", com público alvo crianças entre 8 a 10 anos de idade. As fases do game apresenta situações de risco de acidente na rotina diária da criança, e explica de forma simples e interativa como evitá-los. O game é composto por dez cenários, sendo quatro de orientações e seis dos ambientes domiciliar, escolar, via e parque público, onde são expostas as situações de risco de acidente. Conclusão: O uso de ferramentas tecnológicas nas ações de educação em saúde, permite que a criança seja protagonista no cuidado com sua saúde e bem-estar, o despertar do senso crítico compatível com sua faixa etária, pode contribuir na redução de acidentes na infância. (AU)


Objective: To describe the development of an educational game prototype for the prevention of common accidents in childhood. Methods: Descriptive study on technological development of an educational game prototype, from original research on the use of gamification as an educational tool for the prevention of childhood accidents. The development of the prototype was guided by the Design Thinking strategy, using scratch educational software which is a free online platform. The scenarios and characters were built with figures available in free internet access. Results: The prototype of the game was titled "Danger Detectives", with audience targeting children between 8 to 10 years of age. The phases of the game presents situations of risk of accident in the daily routine of the child and explains in a simple and interactive way how to avoid them. The game consists of ten scenarios, four of which are guidelines and six of the home, school, road and public park environments, where accident risk situations are exposed. Conclusion: The use of technological tools in health education actions allows the child to be a protagonist in the care of their health and well-being, the awakening of critical sense compatible with their age group can contribute to the reduction of accidents in childhood. (AU)


Objetivo: Describir el desarrollo de un prototipo de juego educativo para la prevención de accidentes comunes en la infância. Methods: Estudio descriptivo sobre desarrollo tecnológico de un prototipo de juego educativo, derivado de la investigación original sobre el uso de la gamificación como herramienta educativa para la prevención de accidentes infantiles. El desarrollo del prototipo fue guiado por la estrategia Design Thinking, utilizando software educativo scratch que es una plataforma en línea gratuita. Los escenarios y personajes fueron construidos con figuras disponibles en acceso gratuito a Internet. Resultados: El prototipo del juego se tituló "Detectives de peligro", con un público dirigido a niños de entre 8 y 10 años de edad. Las fases del juego presentan situaciones de riesgo de accidente en la rutina diaria del niño, y explican de una manera sencilla e interactiva cómo evitarlos. El juego consta de diez escenarios, cuatro de los cuales son pautas y seis de los ambientes de hogar, escuela, carretera y parque público, donde las situaciones de riesgo de accidentes están expuestas. Conclusión: El uso de herramientas tecnológicas en las acciones de educación sanitaria permite al niño ser protagonista en el cuidado de su salud y bienestar, el despertar del sentido crítico compatible con su grupo de edad puede contribuir a la reducción de los accidentes en la infancia. (AU)


Assuntos
Prevenção de Acidentes , Jogos e Brinquedos , Saúde da Criança , Educação em Saúde , Informática em Enfermagem
4.
Braz J Biol ; 83: e244784, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34190759

RESUMO

The work aimed to evaluate the weight-length relationship and the condition factor, characterizing the biometry, anatomy, histology and volumetric density of the liver of Brycon amazonicus, in different stages of body growth. The experiment used twenty specimens in four stages of body growth (PI, PII, PIII and PIV) harvested every 90 days, containing five specimens, each group. The livers were dissected, weighed (g) and processed routinely using the hematoxylin and eosin technique. The data were submitted to analysis of variance, Pearson's correlation test and linear regression. The equation that represented the weight-length relation was W = 0.05902 x L2.63, with negative allometric growth, but with a relative condition factor equal to 1.0. The liver was divided into three lobes with the gallbladder close to the right lobe and its color varied from light red to dark red, not varying in relation to other fish species. The hepatosomatic relationship followed body growth until the PII group stage and then declined, demonstrating the behavior of its development in Brycon amazonicus. The organ consists predominantly of hepatocytes, followed by sinusoidal vessels and capillaries, with histological morphology similar to that of many species of fish. Melanomacrophage centers were found only in the most developed animals, but in small quantities, prompting the development of new research on this cell, in this species. In this way, research of this nature allows the characterization of fish species, helping to improve breeding methods, understanding pathological processes caused by diseases, and obtaining better productive capacity, serving an increasingly demanding and prosperous market.


Assuntos
Caraciformes , Animais , Fígado
5.
Braz. j. biol ; 81(2): 251-257, Jan.-May 2021. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1153347

RESUMO

Abstract Genetically modified plants are one of the tactics used in integrated pest management - IPM. There is great concern about the impact of these plants on non-target organisms. On the other hand, there is little information in the literature on the effects of transgenics (Bacillus thuringiensis) Bt on populations of phytophagous mites, and the physiological responses that this attack promotes on plants. The objective of this work was to evaluate the biology of the T. ludeni mite in Bt cotton, expressing the Cry1F and Cry1Ac proteins. To evaluate the behavior of food and oviposition preference of the T. ludeni with Bt cotton and isohybrid. Verify if the physiological stress caused by T. ludeni's attack is differentiated in Bt cotton. The mites were reared in Bt cotton and isohybrid, in a total of 40 replicates in the completely randomized design and the biological cycle was evaluated. The food preference and oviposition analysis were done with 10 replicates, with choice. The physiological stress was evaluated through chlorophyll fluorescence, under greenhouse conditions. The data of the T. ludeni biology were analyzed by Student's t-test, for food and oviposition preference the chi-square test was performed. Regression models were fitted for the fluorescence parameters. The model identity test was used to evaluate the differences between Bt and isohybrid treatments. Cry1F and Cry1Ac proteins have not affected the biology of T. ludeni. The photosynthetic parameters in Bt cotton plants were less influenced by T. ludeni infestation.


Resumo O uso de plantas geneticamente modificadas é uma das táticas utilizadas no manejo integrado de pragas - MIP. Observa-se grande preocupação com o impacto dessas plantas sobre organismos não alvos. Por outro lado, existe pouca informação na literatura sobre efeitos dos transgênicos (Bacillus thuringiensis) Bt em populações de ácaros fitófagos, e as respostas fisiológicas que esse ataque promove nas plantas. Objetivou-se com esse trabalho avaliar a biologia do ácaro T. ludeni em algodoeiro Bt, expressando as proteínas Cry1F e Cry1Ac. Avaliar se há comportamento de preferência alimentar e postura de T. ludeni em relação ao algodoeiro Bt e seu iso-híbrido. E verificar se o estresse fisiológico causado pelo ataque de T. ludeni é diferenciado em algodoeiro Bt. Os ácaros foram criados em algodoeiro Bt e iso-híbrido, em um total de 40 repetições no delineamento inteiramente casualizado, onde foi avaliado o ciclo biológico. A análise de preferência alimentar e de posturas foi feita com 10 repetições, com escolha. O estresse fisiológico foi avaliando através da fluorescência da clorofila, em casa de vegetação. Os dados da biologia de T. ludeni foram analisados pelo teste t Student, para preferência alimentar e postura foi realizado o teste qui-quadrado. Para os parâmetros da fluorescência, foram ajustados modelos de regressão. Para testar as diferenças entre Bt e iso-híbrido foi utilizado o teste de identidade de modelos. As proteínas Cry1F e Cry1Ac não afetaram a biologia de T. ludeni. Os parâmetros fotossintéticos em plantas de algodoeiro Bt foram menos influenciados pela infestação de T. ludeni.

6.
J Laryngol Otol ; 135(5): 420-425, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33952362

RESUMO

OBJECTIVE: Tympanostomy is one of the most commonly performed surgical procedures in otolaryngology, and its complexity is challenging for trainee surgeons. Investing in medical education is a cornerstone of good patient safety practices. For trainees, use of simulators before operating on actual patients helps mitigate risks. This study aimed to develop a three-dimensional printed model simulator for myringotomy, tympanostomy and ventilation tube placement. METHODS: An articulated model with a detachable portion, base and plastic bag to simulate the external auditory canal, middle ear and tympanic membrane, respectively, was modelled and printed. RESULTS: The final simulator was made from acrylonitrile butadiene styrene polymer and measured 4 × 4 × 12 cm. It was designed to mimic the angulation of patient anatomy in the myringotomy position and simulate the texture and colour of the tissues of interest. The cost was low, and testing with an operating microscope and endoscope yielded satisfactory results. The advent of three-dimensional printing technology has made surgical simulation more accessible and less expensive, providing several advantages for medical education. CONCLUSION: The proposed model fulfilled expectations as a safe, inexpensive, reproducible, user-friendly and accessible surgical education tool that can be improved and reassessed for further research.


Assuntos
Endoscopia/educação , Microcirurgia/educação , Ventilação da Orelha Média/educação , Modelos Anatômicos , Impressão Tridimensional , Treinamento por Simulação , Adulto , Criança , Pré-Escolar , Competência Clínica , Humanos , Internato e Residência
7.
Toxicon ; 198: 171-175, 2021 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-34029603

RESUMO

Photobiomodulation using light-emitting diode (LED) treatment has analgesic and anti-inflammatory effects which can be an effective therapeutic associated with serum therapy for local treatment of snakebites. Here we explored the effects of LED treatment on isolated macrophage under Bothrops jararacussu venom. Results showed that LED induced IL-6 and TNF-α genes down-regulation and, TGF and ARG1 genes up-regulation which indicates a polarization of macrophages to an M2 phenotype contributing to both tissue repair and resolution of inflammation.


Assuntos
Bothrops , Venenos de Crotalídeos , Terapia com Luz de Baixa Intensidade , Animais , Macrófagos , Camundongos , Fenótipo
8.
Clin Transl Oncol ; 23(9): 1955-1960, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33840046

RESUMO

INTRODUCTION: The aim of this study was to analyze the associations between perceived social support and sociodemographic variables on coping strategies. METHODS: A prospective, cross-sectional, multicenter study was conducted in 404 women with resected, non-metastatic breast cancer. Participants completed questionnaires: perceived social support (MSPSS), coping strategies (Mini-MAC), and psychological distress (BSI-18). RESULTS: Sociodemographic factors as age, education, and partnership status were associated with coping strategies. As for maladaptive strategies, hopelessness was more frequent in older people and lower educational level; fatalism in older and single people, and cognitive avoidance was associated with lower educational level. Suppor t from family, friends, and partners was associated with a greater fighting spirit. In contrast, high psychological distress (anxiety and depression) was associated with greater use of maladaptive strategies. CONCLUSION: Young people, a high level of education, having a partner, low psychological distress, and seeking social support were associated with the use of adaptive cancer coping strategies.

9.
Rev. SOBECC ; 26(1): 21-26, 31-03-2021.
Artigo em Inglês | LILACS | ID: biblio-1177427

RESUMO

Objetivo: Identificar a percepção de enfermeiros do bloco operatório sobre os motivos para o cancelamento de cirurgias eletivas e as estratégias para reduzir as taxas de suspensão. Método: Estudo de abordagem qualitativa realizado em um hospital de alta complexidade do Recife, Pernambuco, Brasil, com enfermeiros atuantes no bloco operatório. A coleta de dados foi realizada por meio de entrevistas gravadas com sete enfermeiros responsáveis pelas cirurgias eletivas. Para o tratamento dos dados, utilizou-se a técnica da análise de conteúdo de Bardin. Resultados: Os discursos foram agrupados em duas categorias temáticas: lacunas no planejamento cirúrgico, com três subcategorias, e prevenção de situações evitáveis, com duas subcategorias. Conclusão: Os motivos para o cancelamento de cirurgias estavam relacionados ao planejamento do ambiente cirúrgico e à preparação clínica do paciente. Os enfermeiros identificaram esses motivos como situações evitáveis e consideraram que a comunicação efetiva e atitudes de liderança assertivas são estratégias que reduzem os índices de suspensão cirúrgica.


Objective: To identify the perception of surgical block nurses of the reasons for the cancellation of elective surgeries and strategies to reduce suspension rates. Method: This is a qualitative study carried out in a tertiary hospital of Recife, Pernambuco, Brazil, with nurses working in the surgical block. Data were collected through recorded interviews with seven nurses responsible for elective surgeries. Data treatment was based on Bardin's content analysis technique. Results: The statements were grouped into two thematic categories: gaps in surgical planning, with three subcategories, and prevention of avoidable situations, with two subcategories. Conclusion: The causes for surgery cancellation were related to surgical environment planning and the patient's clinical preparation. The nurses defined these reasons as avoidable situations and considered effective communication and assertive leadership attitudes as strategies that reduce surgical suspension rates.


Objetivo: Identificar la percepción de los enfermeros en el quirófano sobre los motivos de cancelación de las cirugías electivas y las estrategias para reducir las tasas de suspensión. Método: Estudio cualitativo, realizado en un hospital de alta complejidad en Recife, Pernambuco, Brasil, con enfermeros trabajando en quirófano. La recolección de datos se realizó mediante entrevistas grabadas con siete enfermeros responsables de cirugías electivas. Para el tratamiento de los datos se utilizó la técnica de Análisis de Contenido de Bardin (Bardin Content Analysis). Resultados: Los discursos se agruparon en dos categorías temáticas: brechas en la planificación quirúrgica, con tres subcategorías, y prevención de situaciones evitables, con dos subcategorías. Conclusión: Los motivos para cancelar cirugías estaban relacionados con la planificación del entorno quirúrgico y la preparación clínica del paciente. Los enfermeros identificaron estos motivos como situaciones prevenibles y consideraron que la comunicación eficaz y las actitudes de liderazgo asertivo son estrategias que reducen las tasas de suspensión quirúrgica.


Assuntos
Humanos , Salas Cirúrgicas , Cirurgia Geral , Enfermeiras e Enfermeiros , Prevenção de Doenças , Planejamento , Hospitais
10.
Actas urol. esp ; 45(1): 64-72, ene.-feb. 2021. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-200670

RESUMO

INTRODUCCIÓN Y OBJETIVOS: La vejiga hiperactiva (VH) es un trastorno frecuente que aumenta con la edad y afecta a la calidad de vida de los pacientes. Las guías recomiendan los programas de modificación de la conducta como tratamiento de primera línea, aunque la fisioterapia también se ha utilizado con éxito, seguridad y bajo coste. La fisioterapia clínica está utilizando la electroestimulación transcutánea del nervio tibial (ETNT) y la electroestimulación vaginal (EV). Este estudio tuvo como objetivo evaluar si la combinación de EV con ETNT es más exitosa que la monoterapia con ETNT para el tratamiento de las mujeres con VH. PACIENTES Y MÉTODOS: En total, 106 mujeres mayores de 18 años diagnosticadas con VH o incontinencia urinaria mixta con síntomas típicos de VH se dividieron aleatoriamente en 2 grupos: grupo 1: ETNT (n = 52); grupo 2: ETNT + EV (n = 54). El diario miccional de 3 días, la fuerza muscular del suelo pélvico (escala de Ortiz), el cuestionario de salud de King y el cuestionario de VH se evaluaron antes y después del tratamiento. La variable principal fue la frecuencia urinaria y una reducción de ≥ 3 micciones/día se consideró como clínicamente relevante. Se usaron modelos lineales mixtos para la comparación de los 2 grupos. RESULTADOS: Inicialmente, los grupos fueron similares en edad, índice de masa corporal, número de embarazos, tiempo de aparición de VH y prevalencia de síntomas de VH. Después del tratamiento se observó una reducción en la frecuencia urinaria de 1,5 micciones en el grupo 2; a pesar de ser estadísticamente significativa, esta no fue clínicamente relevante. CONCLUSIONES: La combinación de EV con ETNT no hizo que el tratamiento para la VH fuese más efectivo que la monoterapia con ETNT


INTRODUCTION AND OBJECTIVES: Overactive bladder (OAB) is a prevalent disorder that increases with age and impairs patients' quality of life. Guidelines recommend behavior modifications as the first-line treatment; however, physiotherapy has also been used with success, safety, and low cost. Transcutaneous tibial nerve electrical stimulation (TTNS) and vaginal electrical stimulation (VS) are being used in clinical physiotherapy practice. This study aimed to verify whether the addition of VS to TTNS is more beneficial than TTNS alone for women with OAB. PATIENTS AND METHODS: In all, 106 women aged >18 years diagnosed with OAB or mixed urinary incontinence with prevalent OAB symptoms were randomly divided into 2 groups: Group 1: TTNS (n = 52); Group 2: TTNS + VS (n = 54). The 3 day voiding diary, pelvic floor muscle strength (Ortiz Scale), King's Health Questionnaire, and Overactive Bladder Questionnaire were assessed before and after treatment. Urinary frequency was considered the primary outcome, and a reduction of ≥ 3 micturitions/day was considered clinically relevant. Mixed linear models were used to compare the 2 groups. RESULTS: Initially, the groups were similar in age, body mass index, number of pregnancies, time of OAB onset, and prevalence of OAB symptoms. After treatment, a reduction in urinary frequency of 1.5 micturitions was observed in Group 2, which was not clinically relevant despite being statistically significant. CONCLUSIONS: The addition of VS to TTNS for the treatment of OAB was not more effective than TTNS as a single therapy


Assuntos
Humanos , Feminino , Pessoa de Meia-Idade , Idoso , Bexiga Urinária Hiperativa/terapia , Terapia por Estimulação Elétrica/métodos , Estimulação Elétrica Nervosa Transcutânea/métodos , Vagina , Nervo Tibial , Resultado do Tratamento , Inquéritos e Questionários , Qualidade de Vida , Bexiga Urinária Hiperativa/fisiopatologia
11.
Nat Commun ; 12(1): 1244, 2021 02 23.
Artigo em Inglês | MEDLINE | ID: mdl-33623024

RESUMO

Differentiation between distinct stages is fundamental for the life cycle of intracellular protozoan parasites and for transmission between hosts, requiring stringent spatial and temporal regulation. Here, we apply kinome-wide gene deletion and gene tagging in Leishmania mexicana promastigotes to define protein kinases with life cycle transition roles. Whilst 162 are dispensable, 44 protein kinase genes are refractory to deletion in promastigotes and are likely core genes required for parasite replication. Phenotyping of pooled gene deletion mutants using bar-seq and projection pursuit clustering reveal functional phenotypic groups of protein kinases involved in differentiation from metacyclic promastigote to amastigote, growth and survival in macrophages and mice, colonisation of the sand fly and motility. This unbiased interrogation of protein kinase function in Leishmania allows targeted investigation of organelle-associated signalling pathways required for successful intracellular parasitism.


Assuntos
Diferenciação Celular , Leishmania mexicana/citologia , Leishmania mexicana/enzimologia , Animais , Proteína 9 Associada à CRISPR/metabolismo , Sistemas CRISPR-Cas/genética , Sobrevivência Celular , Feminino , Flagelos/enzimologia , Deleção de Genes , Leishmaniose/parasitologia , Leishmaniose/patologia , Camundongos Endogâmicos BALB C , Modelos Biológicos , Mutação/genética , Proteínas Quinases/genética , Proteínas Quinases/metabolismo , Proteoma/metabolismo , Psychodidae/parasitologia
12.
Braz J Biol ; 81(2): 251-257, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-32844904

RESUMO

Genetically modified plants are one of the tactics used in integrated pest management - IPM. There is great concern about the impact of these plants on non-target organisms. On the other hand, there is little information in the literature on the effects of transgenics (Bacillus thuringiensis) Bt on populations of phytophagous mites, and the physiological responses that this attack promotes on plants. The objective of this work was to evaluate the biology of the T. ludeni mite in Bt cotton, expressing the Cry1F and Cry1Ac proteins. To evaluate the behavior of food and oviposition preference of the T. ludeni with Bt cotton and isohybrid. Verify if the physiological stress caused by T. ludeni's attack is differentiated in Bt cotton. The mites were reared in Bt cotton and isohybrid, in a total of 40 replicates in the completely randomized design and the biological cycle was evaluated. The food preference and oviposition analysis were done with 10 replicates, with choice. The physiological stress was evaluated through chlorophyll fluorescence, under greenhouse conditions. The data of the T. ludeni biology were analyzed by Student's t-test, for food and oviposition preference the chi-square test was performed. Regression models were fitted for the fluorescence parameters. The model identity test was used to evaluate the differences between Bt and isohybrid treatments. Cry1F and Cry1Ac proteins have not affected the biology of T. ludeni. The photosynthetic parameters in Bt cotton plants were less influenced by T. ludeni infestation.


Assuntos
Tetranychidae , Animais , Toxinas de Bacillus thuringiensis , Proteínas de Bactérias/genética , Endotoxinas , Feminino , Gossypium/genética , Proteínas Hemolisinas/genética , Humanos , Larva , Plantas Geneticamente Modificadas/genética , Estresse Fisiológico , Tetranychidae/genética
13.
Chem Biol Interact ; 333: 109347, 2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-33259806

RESUMO

Several reports have suggested that photobiomodulation, owing to its analgesic, anti-inflammatory, and healing effects, may be an effective therapeutic option for local effects of snakebites when the availability and accessibility of conventional serum therapy are inefficient and far from medical care centers. Although there have been studies that demonstrate the application of photobiomodulation in the treatment of local adverse events due to snakebites from snakes of the genus Bothrops, its role in the activation of leukocytes, particularly macrophages, has not been evaluated. Here, we assessed the effect of light-emitting diode (LED) treatment on macrophage activation induced by B. jararacussu venom (BjV). LED treatment caused an increase in the viability of macrophages incubated with BjV. This treatment reduced reactive oxygen species (ROS) and nitric oxide (NO) production by macrophages after incubation with BjV. However, LED treatment did not interfere with IL-1ß and IL-10 production by macrophages after incubation with BjV. In conclusion, this study showed that LED treatment has the potential to be used in combination with conventional serum therapy to prevent or minimize the progression of local to severe symptoms after Bothrops envenomation.


Assuntos
Bothrops , Venenos de Crotalídeos/toxicidade , Terapia com Luz de Baixa Intensidade/instrumentação , Macrófagos/efeitos da radiação , Semicondutores , Mordeduras de Serpentes/imunologia , Mordeduras de Serpentes/radioterapia , Animais , Sobrevivência Celular/efeitos dos fármacos , Interleucina-10/metabolismo , Interleucina-1beta/metabolismo , Espaço Intracelular/efeitos dos fármacos , Espaço Intracelular/metabolismo , Espaço Intracelular/efeitos da radiação , Macrófagos/imunologia , Masculino , Camundongos , Óxido Nítrico/biossíntese , Espécies Reativas de Oxigênio/metabolismo , Mordeduras de Serpentes/metabolismo , Mordeduras de Serpentes/patologia , Superóxidos/metabolismo
14.
Actas Urol Esp (Engl Ed) ; 45(1): 64-72, 2021.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-32660872

RESUMO

INTRODUCTION AND OBJECTIVES: Overactive bladder (OAB) is a prevalent disorder that increases with age and impairs patients' quality of life. Guidelines recommend behavior modifications as the first-line treatment; however, physiotherapy has also been used with success, safety, and low cost. Transcutaneous tibial nerve electrical stimulation (TTNS) and vaginal electrical stimulation (VS) are being used in clinical physiotherapy practice. This study aimed to verify whether the addition of VS to TTNS is more beneficial than TTNS alone for women with OAB. PATIENTS AND METHODS: In all, 106 women aged >18 years diagnosed with OAB or mixed urinary incontinence with prevalent OAB symptoms were randomly divided into 2 groups: Group 1: TTNS (n = 52); Group 2: TTNS + VS (n = 54). The 3 day voiding diary, pelvic floor muscle strength (Ortiz Scale), King's Health Questionnaire, and Overactive Bladder Questionnaire were assessed before and after treatment. Urinary frequency was considered the primary outcome, and a reduction of ≥ 3 micturitions/day was considered clinically relevant. Mixed linear models were used to compare the 2groups. RESULTS: Initially, the groups were similar in age, body mass index, number of pregnancies, time of OAB onset, and prevalence of OAB symptoms. After treatment, a reduction in urinary frequency of 1.5 micturitions was observed in Group 2, which was not clinically relevant despite being statistically significant. CONCLUSIONS: The addition of VS to TTNS for the treatment of OAB was not more effective than TTNS as a single therapy.

15.
Int J Paediatr Dent ; 31(3): 372-382, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-32955769

RESUMO

BACKGROUND: Oral health research in children has focused both on identifying the risk factors for dental caries and on the impact the disease has on children's life. AIM: Identifying studies that used the salutogenic theory to investigate positive aspects to promote a healthy condition in preschool children. DESIGN: A literature scoping review was conducted to answer the question: 'What has been produced in scientific literature about children's oral health with a salutogenic theoretical reference?' The checklist preferred reporting items for systematic reviews and meta-analyses extension for scoping reviews (PRISMA-ScR) was applied. The research was conducted on the databases PubMed, Scopus, and Web of Science with no language, design, country or date restrictions. Three independent reviewers selected the studies to be included in the research, extracted data, and assessed the studies' contribution to the review. RESULTS: Among the 38 studies retained, 10 were included, those were published between 2009 and 2018: five in Brazil, two in the United States of America, and one in Canada, China and India, respectively. All articles were published in English language. Nine studies investigated the relation between the parents'/caretakers' sense of coherence and aspects related to the children's oral health condition. One study investigated health factors among the parents of caries-free children. CONCLUSION: Salutogenic orientation must be included in oral health research, so that salutary and risk factors may coexist, contributing to a new perspective for oral health promotion among children.


Assuntos
Cárie Dentária , Senso de Coerência , Brasil , Pré-Escolar , China , Cárie Dentária/epidemiologia , Humanos , Índia , Saúde Bucal
16.
Domest Anim Endocrinol ; 74: 106526, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-32810656

RESUMO

This study investigated the effects of growth rates and compensatory growth on puberty attainment in Nellore heifers. Nellore heifers (n = 120), weaned at 8 ± 0.75 mo of age, were blocked by sire and BW (180 ± 8.6 kg) and assigned randomly to receive 1 of 4 treatments over a 10-mo period. Treatments included ad libitum feeding (high gain, HG), feed intake to gain 0.6 kg/d (medium gain, MG), restricted feeding (0.2 kg/d) for 4 mo followed by ad libitum feeding for 6 mo (compensatory gain, CG), and alternating periods of ad libitum and restricted feeding for 2 mo each throughout the trial (alternated CG, ACG). Puberty was assessed weekly by transrectal ultrasonography. Blood samples were collected at 8, 11, and 18 mo of age and at puberty to determine circulating concentrations of leptin. At 18 mo of age, nonpubertal heifers were treated with a puberty induction protocol using an intravaginal progestin device. There was no treatment effect (P = 0.17) on the percentage of heifers pubertal by 18 mo of age (HG: 66, MG: 40, CG: 58, and ACG: 52%), BW at puberty, and age at puberty. However, HG heifers had higher ADG (P < 0.01), dry matter intake (P < 0.01), and leptin concentrations (P = 0.03) than heifers from other groups. The response to the puberty induction protocol was similar (P = 0.90) among treatments. Regarding sire effects (genetic effects), there was an effect (P = 0.03) on the percentage of heifers pubertal by 18 mo of age and a tendency (P = 0.07) of sire effect in response to the puberty induction protocol. Compensatory growth appears to be an effective managerial approach to decrease feeding costs and stimulate puberty in Nellore heifers.

17.
Cien Saude Colet ; 25(12): 4875-4886, 2020 Dec.
Artigo em Português, Inglês | MEDLINE | ID: mdl-33295508

RESUMO

Celebrating the 25 years of existence of the Journal Ciência & Saúde Coletiva (C&SC), this paper analyzed 375 documents published between 2000-2019 as an integral part of the editorial of collective oral health. The production analysis aimed to understand how oral health core appears in publications and how it could have contributed to knowledge on the population's health-disease, specific public policies, education, and management of oral health services in the SUS. The process employed bibliometric and documental analysis. We could show the authors' territorial distribution, their extensive collaboration network, and the dimension of citations in publications, including the international plan. The Brazilian states most present in the publications were São Paulo and Minas Gerais, followed by authors from Pernambuco, Rio Grande do Sul, and Santa Catarina. Citations were more frequent in Brazil (85.14%), followed by the United States (2.31%), Portugal (1.34%), and Australia (1.34%). We concluded that, despite the limitations, the C&SC showed unequivocally a powerful instrument for the dissemination of scientific production from the perspective of collective oral health, enabling the exchange of information and facilitating the integration between researchers and enabling a path to its consolidation.


Assuntos
Saúde Bucal , Redação , Austrália , Brasil , Humanos , Portugal
18.
Rev. esp. anestesiol. reanim ; 67(10): 559-562, dic. 2020. ilus
Artigo em Espanhol | IBECS | ID: ibc-200725

RESUMO

El embolismo gaseoso arterial cerebral es un episodio grave y frecuentemente iatrogénico fatal, asociado a procedimientos invasivos. Se trata de una causa posible de paro cardiaco, y el diagnóstico es difícil. Reportamos un caso de paro cardiaco tras una embolia gaseosa en arteria cerebral, en un varón de 63 años sometido a biopsia por aspiración transtorácica con aguja ecoguiada por TAC, que fue exitosamente tratado con oxigenoterapia hiperbárica


Cerebral arterial gas embolism is a serious and often iatrogenic fatal event associated with invasive procedures. It is a possible cause of a cardiac arrest and the diagnosis is challenging. We report a case of a cardiac arrest after a cerebral arterial gas embolism, in a 63-year-old male subjected to a Computed Tomography-guided Transthoracic Needle Aspiration Biopsy, which was successfully managed with hyperbaric oxygen therapy


Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Biópsia Guiada por Imagem/efeitos adversos , Embolia Intracraniana/complicações , Embolia Aérea/complicações , Parada Cardíaca/etiologia , Tomografia Computadorizada por Raios X , Embolia Intracraniana/diagnóstico por imagem
19.
Ciênc. Saúde Colet ; 25(12): 4875-4886, Dec. 2020. graf
Artigo em Português | LILACS-Express | LILACS, Coleciona SUS, Sec. Est. Saúde SP | ID: biblio-1142710

RESUMO

Resumo Comemorando os 25 anos da Revista Ciência & Saúde Coletiva (C&SC), o presente artigo analisou 375 documentos publicados entre 2000-2019 neste periódico, como parte integrante da editoria de saúde bucal coletiva. A análise da produção visou compreender como o núcleo de saúde bucal aparece nas publicações e como poderia ter contribuído com o conhecimento sobre a saúde-doença da população, as políticas públicas específicas, o ensino e a gestão dos serviços de saúde bucal no SUS. O processo privilegiou a análise bibliométrica e a de documentos. Foi possível explicitar a distribuição institucional dos autores, sua expressiva rede de colaboradores e a dimensão das citações das publicações inclusive no plano internacional. Os estados brasileiros mais presentes nas publicações foram São Paulo e Minas Gerais, seguidos por autores de Pernambuco, Rio Grande do Sul e Santa Catarina. As citações foram mais frequentemente do Brasil (85,14%), seguido por Estados Unidos (2,31%), Portugal (1,34%) e Austrália (1,34%). Conclui-se que, mesmo com limitações, C&SC se mostrou, inequivocamente, um potente instrumento de divulgação da produção científica na perspectiva da saúde bucal coletiva, possibilitando a divulgação e o intercâmbio de informações, facilitando a integração entre os pesquisadores e possibilitando um caminho da sua consolidação.


Abstract Celebrating the 25 years of existence of the Journal Ciência & Saúde Coletiva (C&SC), this paper analyzed 375 documents published between 2000-2019 as an integral part of the editorial of collective oral health. The production analysis aimed to understand how oral health core appears in publications and how it could have contributed to knowledge on the population's health-disease, specific public policies, education, and management of oral health services in the SUS. The process employed bibliometric and documental analysis. We could show the authors' territorial distribution, their extensive collaboration network, and the dimension of citations in publications, including the international plan. The Brazilian states most present in the publications were São Paulo and Minas Gerais, followed by authors from Pernambuco, Rio Grande do Sul, and Santa Catarina. Citations were more frequent in Brazil (85.14%), followed by the United States (2.31%), Portugal (1.34%), and Australia (1.34%). We concluded that, despite the limitations, the C&SC showed unequivocally a powerful instrument for the dissemination of scientific production from the perspective of collective oral health, enabling the exchange of information and facilitating the integration between researchers and enabling a path to its consolidation.

20.
Arq. bras. med. vet. zootec. (Online) ; 72(6): 2271-2278, Nov.-Dec. 2020. graf
Artigo em Português | LILACS-Express | LILACS | ID: biblio-1142300

RESUMO

RESUMO Relata-se um caso de melanoma oral disseminado em uma cadela de dois anos, com protrusão de bulbo ocular unilateral e quadro convulsivo progressivo. Os exames de imagem revelaram aumento de volume nas regiões submandibular, maxilar e cerebral, padrão nodular pulmonar e aumento das dimensões ovarianas. A citologia da massa submandibular indicou proliferação epitelial maligna, enquanto a biópsia excisional foi sugestiva de melanoma amelanótico. Na necropsia, havia uma massa gengival localmente infiltrativa e nodulações brancas nos linfonodos, nos rins, no pulmão, no cérebro e nos ovários, indicativas de metástase. O diagnóstico histopatológico consistiu de neoplasia maligna metastática indiferenciada, indicativo de melanoma amelanótico. Células caracterizadas por núcleo com cromatina espessa, múltiplos nucléolos bem evidentes, mitoses atípicas e multinucleações consistiram nos principais critérios de malignidade. No espaço peritrabecular ósseo facial, havia rara diferenciação pigmentar melanocítica, confirmada histoquimicamente pela técnica de Fontana-Massom e Giemsa. Algumas células foram positivas pela imuno-histoquímica para PNL-2 e Melan-A, e o diagnóstico de melanoma amelanótico disseminado foi firmado. A indiferenciação neoplásica marcante, com disseminação metastática multissistêmica e acometimento mútuo de sítios anatômicos pouco comuns, conjuntamente com a ampla variação dos padrões celulares, foi responsável pelo desafio diagnóstico do presente caso, ressaltando o papel decisivo da imuno-histoquímica para confirmação diagnóstica. A importância clínica deste trabalho consiste ainda em alertar a comunidade clínica e científica acerca da dificuldade diagnóstica, devendo-se considerar o melanoma amelanótico como diferencial mesmo em casos de lesões orais menos perceptíveis e/ou desprovidas de pigmentação.


ABSTRACT A case of disseminated oral melanoma in a two year old female dog with unilateral protuberance of the eye bulb and progressive seizure is described. Imaging exams revealed increase of the submandibular, maxillary and cerebral regions, nodular pattern in lungs and increased ovarian dimensions. The cytology of the submandibular mass indicated a malignant epithelial proliferation, whereas the excisional biopsy indicated an amelanotic melanoma. At necropsy, a locally infiltrating gingival mass and white nodules in the lymph nodes, kidneys, lung, brain and ovaries were observed, indicative of metastases. Histopathological diagnosis consisted of an undifferentiated malignant metastatic neoplasm. Nucleus with coarse chromatin, prominent nucleoli, bizarre mitotic figures and multinucleated cells were the major malignant features. There was a poor melanocytic pigment differentiation in the peritrabecular space of facial bones, confirmed by Fontana-Masson and Giemsa histochemical techniques. Only a few cells were immunohistochemically positive for PNL-2 and Melan-A and the diagnosis of a disseminated amelanotic melanoma was performed. The diagnostic challenge was based on marked neoplastic undifferentiation, with multisystemic metastasis and mutual involvement of uncommon anatomic sites, associated with a large variability of cellular patterns, highlighting the decisive role of immunohistochemistry for diagnostic confirmation. Therefore, the clinical importance of this study is to warn the clinical and scientific community about the diagnostic challenge, considering the amelanotic melanoma as a differential even in cases of poorly apparent and/or nonpigmented oral lesions.

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