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1.
Rev. Paul. Pediatr. (Ed. Port., Online) ; 40: e2020205, 2022. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1180891

RESUMO

ABSTRACT Objective: To investigate the adequacy of the theoretical model of the Movement Assessment Battery for Children-Second Edition (MABC-2) instrument. Methods: 582 children, of both sexes, aged between 3 and 5 years and residents in the city of Maringá (state of Paraná, Southern Brazil) participated in the study. Data were collected from May/2014 to June/2015 and analyzed using descriptive and inferential statistics. Results: The evidence obtained from exploratory factor analysis indicated the presence of two factors, which was the option that best fitted the explanatory model. Hence, it was necessary to regroup the motor tasks of the dimensions "Aiming & catching" and "Balance" into only one dimension. It is noteworthy that the "Bicycle trail" motor task did not fit the model, as it presented a low and negative factor load in the analyzed dimensions. In the confirmatory factor analysis, adequate adjustment indices were observed for the tested model, which confirmed the non-classification of the "Bicycle trail" motor task in the original dimension. Conclusions: After removing the "Bicycle trail" motor task, the adjusted two-factor model seems to be the most appropriate to assess the motor performance of children participating in the study.


RESUMO Objetivo: Investigar a adequabilidade do modelo teórico do instrumento de avaliação motora Movement Assessment Battery for Children-Second Edition. Métodos: Participaram do estudo 582 crianças, de ambos os sexos com idade entre 3 e 5 anos da cidade de Maringá, Paraná, no período de maio/2014 a junho/2015. Para analisar os dados, utilizou-se a estatística descritiva e inferencial. Resultados: As evidências obtidas por meio da análise fatorial exploratória indicaram a presença de dois fatores. Essa opção foi a que melhor se ajustou ao modelo explicativo. Com isso, foi necessário reagrupar as tarefas motoras das dimensões "lançar e receber" e "equilíbrio" em apenas uma dimensão. Destaca-se que a tarefa motora "caminho da bicicleta" não se adequou ao modelo, pois apresentou carga fatorial baixa e negativa nas dimensões analisadas. Na análise fatorial confirmatória, observaram-se índices de ajustamento adequados para o modelo testado, a qual confirmou o não enquadramento da tarefa motora "caminho da bicicleta" na dimensão original. Conclusões: Após a retirada da tarefa motora "caminho da bicicleta", o modelo ajustado de dois fatores parece ser o mais adequado para avaliar o desempenho motor das crianças participantes do estudo.

2.
Rev Paul Pediatr ; 40: e2020205, 2021.
Artigo em Inglês, Português | MEDLINE | ID: mdl-33825767

RESUMO

OBJECTIVE: To investigate the adequacy of the theoretical model of the Movement Assessment Battery for Children-Second Edition (MABC-2) instrument. METHODS: 582 children, of both sexes, aged between 3 and 5 years and residents in the city of Maringá (state of Paraná, Southern Brazil) participated in the study. Data were collected from May/2014 to June/2015 and analyzed using descriptive and inferential statistics. RESULTS: The evidence obtained from exploratory factor analysis indicated the presence of two factors, which was the option that best fitted the explanatory model. Hence, it was necessary to regroup the motor tasks of the dimensions "Aiming & catching" and "Balance" into only one dimension. It is noteworthy that the "Bicycle trail" motor task did not fit the model, as it presented a low and negative factor load in the analyzed dimensions. In the confirmatory factor analysis, adequate adjustment indices were observed for the tested model, which confirmed the non-classification of the "Bicycle trail" motor task in the original dimension. CONCLUSIONS: After removing the "Bicycle trail" motor task, the adjusted two-factor model seems to be the most appropriate to assess the motor performance of children participating in the study.

3.
Oncol Rep ; 45(2): 652-664, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33416171

RESUMO

Osteopontin (OPN) is upregulated in several types of tumor and has been associated with chemoresistance. However, the contribution of OPN splicing isoforms (OPN­SIs) to chemoresistance requires further investigation. The present study aimed to evaluate the expression patterns of each tested OPN­SI in cisplatin (CDDP)­resistant ovarian carcinoma cell lines, focusing on the role of the OPN­c isoform (OPNc) in drug resistance. ACRP ovarian cancer cells resistant to CDDP, as well as their parental cell line A2780, were used. Analyses of the transcriptional expression of OPN­SIs, epithelial­mesenchymal transition (EMT) markers and EMT­related cytokines were performed using reverse transcription­quantitative PCR. OPNc was silenced in ACRP cells using anti­OPNc DNA oligomers and stably overexpressed by transfecting A2780 cells with a mammalian expression vector containing the full length OPNc cDNA. Functional assays were performed to determine cell proliferation, viability and colony formation. The results demonstrated that among the three tested OPN­SIs, OPNc was the most upregulated transcript in the ACRP cells compared with the parental A2780 cells. In addition, the expression levels of P­glycoprotein multidrug transporter were upregulated in CDDP­resistant ACRP cells compared with those in A2780 cells. OPNc knockdown sensitized ACRP cells to CDDP treatment and downregulated P­gp expression levels compared with those in the negative control group. Additionally, silencing of OPNc impaired cell proliferative and colony formation abilities, as well as reversed the expression levels of EMT markers and EMT­related cytokines compared with those in the negative control cells. Notably, although stable OPNc overexpression resulted in increased A2780 cell proliferation, it notably increased CDDP sensitivity compared with that in the cells transfected with a control vector. These results suggested that OPNc silencing may represent a putative approach to sensitize resistant ovarian cancer cells to chemotherapeutic agents.

4.
PLoS Negl Trop Dis ; 14(12): e0009015, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-33370305

RESUMO

Trypanosoma rangeli is a non-pathogenic protozoan parasite that infects mammals, including humans, in Chagas disease-endemic areas of South and Central America. The parasite is transmitted to a mammalian host when an infected triatomine injects metacyclic trypomastigotes into the host's skin during a bloodmeal. Infected mammals behave as parasite reservoirs for several months and despite intensive research, some major aspects of T. rangeli-vertebrate interactions are still poorly understood. In particular, many questions still remain unanswered, e.g. parasite survival and development inside vertebrates, as no parasite multiplication sites have yet been identified. The present study used an insect bite transmission strategy to investigate whether the vector inoculation spot in the skin behave as a parasite-replication site. Histological data from the skin identified extracellular parasites in the dermis and hypodermis of infected mice in the first 24 hours post-infection, as well as the presence of inflammatory infiltrates in a period of up to 7 days. However, qPCR analyses demonstrated that T. rangeli is eliminated from the skin after 7 days of infection despite being still consistently found on circulating blood and secondary lymphoid tissues for up to 30 days post-infection. Interestingly, significant numbers of parasites were found in the spleen and mesenteric lymph nodes of infected mice during different periods of infection and steady basal numbers of flagellates are maintained in the host's bloodstream, which might behave as a transmission source to insect vectors. The presence of parasites in the spleen was confirmed by fluorescent photomicrography of free and cell-associated T. rangeli forms. Altogether our results suggest that this organ could possibly behave as a T. rangeli maintenance hotspot in vertebrates.


Assuntos
Doença de Chagas/transmissão , Linfonodos/parasitologia , Pele/parasitologia , Baço/parasitologia , Trypanosoma rangeli/isolamento & purificação , Animais , América Central/epidemiologia , Doença de Chagas/epidemiologia , Modelos Animais de Doenças , Interações Hospedeiro-Parasita , Humanos , Mordeduras e Picadas de Insetos/parasitologia , Insetos Vetores/parasitologia , Camundongos , Rhodnius/parasitologia , Sepse/parasitologia , América do Sul/epidemiologia
5.
Cad. Bras. Ter. Ocup ; 28(4): 1180-1192, Oct.-Dec. 2020. tab
Artigo em Português | LILACS-Express | LILACS, Index Psicologia - Periódicos técnico-científicos | ID: biblio-1153631

RESUMO

Resumo Objetivo Acompanhar, ao longo de 24 meses, as alterações entre o diagnóstico de Desenvolvimento Motor Típico (DM) e o provável Transtorno do Desenvolvimento da Coordenação (pTDC) em pré-escolares. Método Foram elaborados dois designs de coleta: 1) Design longitudinal de 12 meses (Long-1), constituído por 201 crianças, com idade inicial média de 3,6±0,5 anos; 2) Design longitudinal de 24 meses (Long-2), composto por 27 crianças, com idade inicial média de 3,6±0,1 anos. Como instrumento de avaliação, foi utilizado o Movement Assessment Battery for Children 2 (MABC-2). Os dados foram analisados usando os testes Kolmogorov-Smirnov, Shapiro-Wilk, Wilcoxon e Friedman, adotando p<0,05. Resultados Os resultados do DM no Long-1 revelaram alta prevalência do pTDC de 24,4% na avaliação inicial e 19,4% após 12 meses. No DM, observou-se aumento na destreza manual e diminuição na habilidade de lançar e receber após 12 meses. Nas tarefas de destreza manual, as meninas foram superiores aos meninos após 12 meses, sendo os meninos superiores na tarefa de lançar e receber nas duas avaliações. Os resultados do DM no Long-2 evidenciaram prevalência de 18,5% de pTDC na primeira avaliação, de 7,4% após 12 meses e 22,2% após 24 meses. Ocorreu aumento do escore na destreza manual ao longo dos 24 meses, no entanto, ocorreu declínio nas tarefas de equilíbrio entre as 3 avaliações. Conclusão O desenvolvimento motor típico e o provável Transtorno do Desenvolvimento da Coordenação não apresentaram constância e consistência entre as avaliações motoras ao longo de 24 meses, demonstrando oscilação na classificação de provável transtorno de desenvolvimento da coordenação e/ou do desenvolvimento motor típico.


Abstract Objective To follow changes in the diagnosis of Typical Motor Development (MD) and the probable Coordination development disorder (pCDD) diagnostics in preschoolers for 24 months. Method Two data collection designs were elaborated: 1) Longitudinal design of 12 months (Long-1) consisting of 201 children, with an average initial age of 3.6 ± 0.5 years old; 2) Longitudinal design of 24 months (Long-2) composed of 27 children, with an average initial age of 3.6±0.5 years old. The assessment tool used was the Movement Assessment Battery for Children 2 (MABC-2). For data analysis, the Kolmogorov-Smirnov, Shapiro-Wilk, Wilcoxon, and Friedman tests were used, adopting p <0.05. Results The results of MD in Long-1 revealed a high prevalence of probable pCDD with 24.4% in the 1st evaluation and 19.4% in the 2nd evaluation. In the MD, we observed a manual dexterity improvement and a reduced ability to throw and receive during the 12 months. In manual dexterity tasks, girls were better than boys in the 2nd evaluation, and for the task of throwing and receiving boys were better at two of the evaluations. The results of MD in Long-2 showed a prevalence of 18.5% of pTDC in the first evaluation, 7.4% after 12 months, and 22.2% after 24 months. There was an increase in the score in manual dexterity over the 24 months, however, there was a decline in the balance tasks between the 3 assessments. Conclusion It is concluded that the typical DM and the probable DCD did not show constant and consistent development during motor evaluations over 24 months, showing oscillation in the classification of probable coordination development disorder and/or typical motor development.

6.
Arch Virol ; 2020 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-33159590

RESUMO

Pseudocowpox is a zoonosis caused by pseudocowpox virus (PCPV), which mainly affects cows but can be an occupational disease of humans. The aim of the study was to validate a quantitative polymerase chain reaction (qPCR) assay for the detection of PCPV. The assay was able to detect up to 1000 copies of PCPV per µL in field samples, with a sensitivity of 80% and a specificity of 100%. We did not observe any cross-reactivity between PCPV-positive samples and samples that were positive for other genetically similar viruses. The repeatability and reproducibility were adequate according to parameters preestablished in official test validation manuals.

7.
Mol Biol Rep ; 47(10): 8339-8345, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33006711

RESUMO

Among osteopontin splice variants (OPN-SV), the expression profile of osteopontin-4 (OPN4) and osteopontin-5 (OPN5) has not been addressed in distinct cancer types. We herein aimed to investigate their expression in several cancer cell lines, besides comparing it in relation to the three previously described OPN-SV: OPNa, OPNb and OPNc. Total RNA from cancer cell lines, including prostate (PC3 and DU145), ovarian (A2780), breast (MCF-7 and MDA-MB-231), colorectal (Caco-2, HT-29 and HCT-116), thyroid (TT, TPC1 and 8505c) and lung (A549 and NCI-H460) was extracted, followed by cDNA synthesis. OPN-SV transcript analysis by RT-PCR or RT-qPCR were performed using OPN-SV specific oligonucleotides and gapdh and actin transcripts were used as housekeeping controls. OPN4 and OPN5 transcripts displayed co-expression in most tested cell lines. OPN4 was found expressed in similar or higher levels in relation to OPN5. Moreover, in most tested cell lines, OPN4 is also expressed in similar levels to OPNa or OPNb. The expression of OPN5 is also generally variable in relation to the other OPN-SV, but expressed in similar or higher levels in relation to OPNc, depending on each tested cell line. OPN4 and OPN5 seem to be co-expressed in several tumor types and OPN4 is one of the most overexpressed OPN-SV in distinct tumor cell lines. Once both OPN4 and OPN5 are differentially expressed and also evidence tumor-specific expression patterns, we hypothesize that similarly to the other OPN-SV, they also possibly contribute to key aspects of tumor progression, what should be further functionally investigated in distinct tumor models.

8.
Arq Bras Cardiol ; 115(4): 669-677, 2020 10.
Artigo em Inglês, Português | MEDLINE | ID: mdl-33111868

RESUMO

BACKGROUND: Many studies have shown that the diterpenoid classes exert a significant effect on the cardiovascular system. Diterpenes, in particular, are among the main compound links to cardiovascular properties such as vasorelaxant, inotropic, diuretic and hypotensive activity. While the manool vasorelaxation mechanism is visible, its effect on blood pressure (BP) is still unknown. OBJECTIVE: To evaluate the in vivo hypotensive effect of manool and check the ex vivo vasorelaxation effect in rat aortic rings. METHODS: The animals were divided randomly into two groups: normotensive and hypertensive. The normotensive group was sham-operated, and the 2K1C model was adopted for the hypertensive group. Invasive BP monitoring was performed for manool tests at different doses (10, 20 and 40 mg/kg). Concentration-response curves for manool were obtained in the aorta rings, with endothelium, pre-contracted with phenylephrine (Phe) after incubation with Nω-nitro-L-arginine methyl ester(L-NAME) or oxadiazole [4,3-a]quinoxalin-1-one (ODQ). Nitric oxide (NOx) plasma levels were measured by chemiluminescence assay. RESULTS: After manool administration, BP was reduced in normotensive and hypertensive groups, and this effect was inhibited by L-NAME in hypertensive animals only in 10 mg/kg dose. Ex vivo manool promoted vasorelaxation, which was inhibited by L-NAME and ODQ incubation or endothelium removal. NOx plasma levels increased in the hypertensive group after manool administration. Manool elicits endothelium-dependent vascular relaxation in rat aorta mediated by the NO/cGMP signaling pathway and BP reduction, also by NOx plasma increase. These combined effects could be involved in modulating peripheral resistance, contributing to the antihypertensive effect of diterpene. CONCLUSION: These effects together could be involved in modulating peripheral resistance, contributing to the antihypertensive effect of diterpene.

9.
Int J Mol Sci ; 21(20)2020 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-33086487

RESUMO

In thyroid cancer, calcification is mainly present in classical papillary thyroid carcinoma (PTC) and in medullary thyroid carcinoma (MTC), despite being described in benign lesions and in other subtypes of thyroid carcinomas. Thyroid calcifications are classified according to their diameter and location. At ultrasonography, microcalcifications appear as hyperechoic spots ≤ 1 mm in diameter and can be named as stromal calcification, bone formation, or psammoma bodies (PBs), whereas calcifications > 1 mm are macrocalcifications. The mechanism of their formation is still poorly understood. Microcalcifications are generally accepted as a reliable indicator of malignancy as they mostly represent PBs. In order to progress in terms of the understanding of the mechanisms behind calcification occurring in thyroid tumors in general, and in PTC in particular, we decided to use histopathology as the basis of the possible cellular and molecular mechanisms of calcification formation in thyroid cancer. We explored the involvement of molecules such as runt-related transcription factor-2 (Runx-2), osteonectin/secreted protein acidic and rich in cysteine (SPARC), alkaline phosphatase (ALP), bone sialoprotein (BSP), and osteopontin (OPN) in the formation of calcification. The present review offers a novel insight into the mechanisms underlying the development of calcification in thyroid cancer.

10.
Arq. bras. cardiol ; 115(4): 669-677, out. 2020. tab, graf
Artigo em Português | LILACS, Sec. Est. Saúde SP | ID: biblio-1131333

RESUMO

Resumo Fundamento: Diversos estudos têm mostrado que as classes de diterpenos exercem efeito significativo no sistema cardiovascular. Os diterpenos, em particular, estão entre os principais compostos associados às propriedades cardiovasculares, como a propriedade vasorrelaxante, inotrópica, diurética e a atividade hipotensora. Embora o mecanismo de vasorrelaxamento do manool seja visível, seu efeito sobre a pressão arterial (PA) ainda é desconhecido. Objetivo: Avaliar o efeito hipotensor in vivo do manool e verificar o efeito de vasorrelaxamento ex vivo em anéis aórticos de ratos. Métodos: Os animais foram divididos aleatoriamente em dois grupos: normotensos e hipertensos. O grupo normotenso foi submetido à cirurgia sham e adotou-se o modelo 2R1C para o grupo hipertenso. Realizou-se monitoramento invasivo da PA para testes com manool em diferentes doses (10, 20 e 40 mg/kg). Foram obtidas curvas de concentração-resposta para o manool nos anéis aórticos, com endotélio pré-contraído com fenilefrina (Phe) após incubação com Nω-nitro-L-arginina metil éster (L-NAME) ou oxadiazolo[4,3-a]quinoxalina-1-ona (ODQ). Os níveis plasmáticos de óxido nítrico (NOx) foram medidos por ensaio de quimioluminescência. Resultados: Após a administração de manool, a PA se reduziu nos grupos normotenso e hipertenso, e esse efeito foi inibido pelo L-NAME em animais hipertensos apenas na dose de 10 mg/kg. O manool ex vivo promoveu vasorrelaxamento, inibido pela incubação de L-NAME e ODQ ou remoção do endotélio. Os níveis plasmáticos de NOx aumentaram no grupo hipertenso após a administração de manool. O manool induz o relaxamento vascular dependente do endotélio na aorta de ratos, mediado pela via de sinalização NO/cGMP e redução da PA, e também pelo aumento plasmático de NOx. Esses efeitos combinados podem estar envolvidos na modulação da resistência periférica, contribuindo para o efeito anti-hipertensivo do diterpeno. Conclusão: Esses efeitos em conjunto podem estar envolvidos na modulação da resistência periférica, contribuindo para o efeito anti-hipertensivo do diterpeno.


Abstract Background: Many studies have shown that the diterpenoid classes exert a significant effect on the cardiovascular system. Diterpenes, in particular, are among the main compound links to cardiovascular properties such as vasorelaxant, inotropic, diuretic and hypotensive activity. While the manool vasorelaxation mechanism is visible, its effect on blood pressure (BP) is still unknown. Objective: To evaluate the in vivo hypotensive effect of manool and check the ex vivo vasorelaxation effect in rat aortic rings. Methods: The animals were divided randomly into two groups: normotensive and hypertensive. The normotensive group was sham-operated, and the 2K1C model was adopted for the hypertensive group. Invasive BP monitoring was performed for manool tests at different doses (10, 20 and 40 mg/kg). Concentration-response curves for manool were obtained in the aorta rings, with endothelium, pre-contracted with phenylephrine (Phe) after incubation with Nω-nitro-L-arginine methyl ester(L-NAME) or oxadiazole [4,3-a]quinoxalin-1-one (ODQ). Nitric oxide (NOx) plasma levels were measured by chemiluminescence assay. Results: After manool administration, BP was reduced in normotensive and hypertensive groups, and this effect was inhibited by L-NAME in hypertensive animals only in 10 mg/kg dose. Ex vivo manool promoted vasorelaxation, which was inhibited by L-NAME and ODQ incubation or endothelium removal. NOx plasma levels increased in the hypertensive group after manool administration. Manool elicits endothelium-dependent vascular relaxation in rat aorta mediated by the NO/cGMP signaling pathway and BP reduction, also by NOx plasma increase. These combined effects could be involved in modulating peripheral resistance, contributing to the antihypertensive effect of diterpene. Conclusion: These effects together could be involved in modulating peripheral resistance, contributing to the antihypertensive effect of diterpene.


Assuntos
Animais , Ratos , Pressão Arterial , Hipertensão/tratamento farmacológico , Aorta Torácica , Vasodilatação , Vasodilatadores/farmacologia , Pressão Sanguínea , Endotélio Vascular , Diterpenos/farmacologia , Óxido Nítrico/farmacologia
11.
Int J Biol Macromol ; 162: 599-608, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-32565303

RESUMO

This study investigates conformational aspects of ulvans (F2) and their polycarboxyl derivatives obtained through periodate-chlorite oxidation (C3) followed by DEAE-Sephacel fractioning (C3b and C3c). Size exclusion chromatography coupled with laser light scattering and viscometric detection, in addition to circular dichroism (CD) and molecular modeling analyses, suggested that F2 had a compact sphere conformation with a helical motif as secondary structure. In contrast, all the polycarboxyl ulvans showed a random coil conformation, although C3c (NaSO3- 21.0%; COO- 1.81 mmol·g-1; Mw 18 kg·mol-1) had a more rigid and constrained backbone than C3 (NaSO3- 21.0%; COO- 1.81 mmol·g-1; Mw 49 kg·mol-1), largely due to its higher sulfate and carboxyl content. Despite the higher ionic character of C3c, its anticoagulant activity (ACA), determined by activated partial thromboplastin time (APTT) assay, was not improved compared to that of C3. Moreover, C3b (NaSO3- 14.1%; COO- 1.23 mmol·g-1; Mw 8.1 kg·mol-1) had higher activity than F2 (NaSO3- 20.6.%; COO- 0.36 mmol·g-1; Mw 123 kg·mol-1), even with its lower sulfate content and molar mass. These results suggest that the ACA of polycarboxyl ulvans relies on carboxyl and sulfate content and may depend, in addition, on a proper flexible conformation.

12.
Rev Bras Anestesiol ; 70(1): 3-8, 2020.
Artigo em Português | MEDLINE | ID: mdl-32164996

RESUMO

BACKGROUND: The elderly population is an especially heterogeneous group of patients with a rising number of surgical interventions being performed in the very elderly patient. The aim of this study was to evaluate the correlation between different age strata and functional status with the surgical outcome of the elderly patient. METHODS: Retrospective cohort study conducted in a Surgical Intensive Care Unit (SICU), between 2006 and 2013. A total of 2331 surgical patients ≥ 65 years old were included. Patients were grouped according to age: Older Elderly Group (OEG: 65-85 years old); Very Elderly Group (VEG > 85 years old). Demographic and perioperative data were recorded. Revised Cardiac Risk Index, APACHE II and SAPS II scores were calculated and postoperative complications were documented. Variables were compared on univariate analysis. RESULTS: The incidence of the VEG was 5.4%. This group had a higher proportion of non-elective surgery (22.4% vs. 11.2%, p < 0.001), higher APACHE II (12.0 vs. 10.0, p < 0.001) and SAPS II (26.6 vs. 22.2, p < 0.001) scores, higher incidence of organ failure (24.6% vs. 17.6%, p = 0.048) and a higher mortality rate during SICU (14.0% vs. 5.2%, p = 0.026) and hospital stay (9.3% vs. 5.0%, p = 0.012). CONCLUSION: We found that very elderly patients represented a significant proportion of patients admitted to the SICU. They had higher severity scores with a higher prevalence of organ failure and were more likely to undergo non-elective surgery. They had worse outcomes in regarding mortality during SICU and hospital stay.

13.
Leuk Res ; 91: 106316, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32114371
14.
Rev. bras. anestesiol ; 70(1): 3-8, Jan.-Feb. 2020. tab
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1137143

RESUMO

Abstract Background: The elderly population is an especially heterogeneous group of patients with a rising number of surgical interventions being performed in the very elderly patient. The aim of this study was to evaluate the correlation between different age strata and functional status with the surgical outcome of the elderly patient. Methods: Retrospective cohort study conducted in a Surgical Intensive Care Unit (SICU), between 2006 and 2013. A total of 2331 surgical patients' ≥ 65 years old were included. Patients were grouped according to age: Older Elderly Group (OEG: 65‒85 years old); Very Elderly Group (VEG > 85 years old). Demographic and perioperative data were recorded. Revised Cardiac Risk Index, APACHE II and SAPS II scores were calculated and postoperative complications were documented. Variables were compared on univariate analysis. Results: The incidence of the VEG was 5.4%. This group had a higher proportion of non-elective surgery (22.4% vs. 11.2%, p < 0.001), higher APACHE II (12.0 vs. 10.0, p < 0.001) and SAPS II (26.6 vs. 22.2, p < 0.001) scores, higher incidence of organ failure (24.6% vs. 17.6%, p = 0.048) and a higher mortality rate during SICU (14.0% vs. 5.2%, p = 0.026) and hospital stay (9.3% vs. 5.0%, p = 0.012). Conclusion: We found that very elderly patients represented a significant proportion of patients admitted to the SICU. They had higher severity scores with a higher prevalence of organ failure and were more likely to undergo non-elective surgery. They had worse outcomes in regarding mortality during SICU and hospital stay.


Resumo Introdução: A população idosa envolve um grupo muito heterogêneo de doentes, com um crescente número de doentes muito idosos a serem propostos para cirurgia. O objetivo do presente estudo foi avaliar a relação entre diferentes grupos etários e estados funcionais com os resultados cirúrgicos do doente idoso. Métodos: Estudo retrospectivo de coorte realizado em uma Unidade de Cuidados Intensivos Cirúrgica (UCIC) que incluiu um total de 2331 doentes cirúrgicos com idade ≥ 65 anos, entre 2006 e 2013. Os doentes foram agrupados de acordo com a idade: doentes idosos (65-85 anos); doentes muito idosos (DMI > 85 anos). Dados demográficos e perioperatórios foram registrados. Índice de Risco Cardíaco Revisto, scores de APACHE e SAPS II foram calculados e complicações pós-operatórias, documentadas. As variáveis foram comparadas em análise univariada. Resultados: A incidência de DMI foi de 5,4%. Este grupo foi mais frequentemente submetido à cirurgia não eletiva (22,4%vs.11,2%; p< 0,001), apresentou scores maiores de APACHE II (12,0vs.10,0; p< 0,001) e SAPS II (26,6 vs. 22,2; p< 0,001), maior incidência de insuficiência do órgão (24,6%vs.17,6%; p= 0,048) e uma mortalidade superior na UCIC (14,0%vs.5,2%; p= 0,026) e no hospital (9,3% vs.5,0%; p= 0,012). Discussão: Os piores resultados nos DMI podem refletir uma maior vulnerabilidade a complicações pós-operatórias, possivelmente relacionadas com múltiplas comorbilidades e uma reserva fisiológica diminuídas. Conclusão: Os doentes muito idosos representaram uma porção importante dos doentes admitidos na UCIC, tinham scores de gravidade mais elevados e maior prevalência de falência orgânica e foram mais frequentemente submetidos a cirurgias não eletivas. Tinham piores resultados relativamente à mortalidade durante a permanência na UCIC e no hospital.

15.
Arch Toxicol ; 94(1): 257-271, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31768571

RESUMO

Circadian rhythms disruption can be the cause of chronic diseases. External cues, including therapeutic drugs, have been shown to modulate peripheral-circadian clocks. Since anthracycline cardiotoxicity is associated with loss of mitochondrial function and metabolic remodeling, we investigated whether the energetic failure induced by sub-chronic doxorubicin (DOX) treatment in juvenile mice was associated with persistent disruption of circadian regulators. Juvenile C57BL/6J male mice were subjected to a sub-chronic DOX treatment (4 weekly injections of 5 mg/kg DOX) and several cardiac parameters, as well as circadian-gene expression and acetylation patterns, were analyzed after 6 weeks of recovery time. Complementary experiments were performed with Mouse Embryonic Fibroblasts (MEFs) and Human Embryonic Kidney 293 cells. DOX-treated juvenile mice showed cardiotoxicity markers and persistent alterations of transcriptional- and signaling cardiac circadian homeostasis. The results showed a delayed influence of DOX on gene expression, accompanied by changes in SIRT1-mediated cyclic deacetylation. The mechanism behind DOX interference with the circadian clock was further studied in vitro, in which were observed alterations of circadian-gene expression and increased BMAL1 SIRT1-mediated deacetylation. In conclusion, DOX treatment in juvenile mice resulted in disruption of oscillatory molecular mechanisms including gene expression and acetylation profiles.

16.
Phys Occup Ther Pediatr ; 40(2): 121-133, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31544582

RESUMO

Aim: Parental screening of children's motor skills can be helpful for early identification of Developmental Coordination Disorder (DCD). The present study examined the associations between the Developmental Coordination Disorder Questionnaire - Brazilian version (DCDQ-BR) and motor competence in school-age children.Methods: 707 children (332 boys, 375 girls) aged between 6- to 10-years and one of their parents participated in the study. Parents completed the DCDQ-BR and children's motor competence was determined using the Bruininks-Oseretsky Test of Motor Proficiency - 2nd edition (BOT-2).Results: The agreement between the BOT-2 and DCDQ-BR results was comparable across age, ranging from 74-84 percent. In general, there were low- to moderate correlations between all aspects of the parent report and children's motor competence. Sensitivity and specificity of the DCDQ-BR were 70% and 81%, respectively. Overall, boys had higher motor competence than girls, but parent reports were similar for gender.Conclusions: Parental assessment of their child's motor ability is moderately associated with motor competence, and this association is similar for boys and girls, even though boys scored higher in motor competence. Our findings suggest that it may be possible to reduce the length of clinical assessment by only testing children that flag as suspect for DCD in the DCDQ-BR.


Assuntos
Deficiências do Desenvolvimento/diagnóstico , Transtornos das Habilidades Motoras/diagnóstico , Inquéritos e Questionários , Fatores Etários , Brasil , Criança , Feminino , Humanos , Masculino , Reprodutibilidade dos Testes , Fatores Sexuais
17.
Rev. bras. cineantropom. desempenho hum ; 22: e65027, 2020. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1092437

RESUMO

Abstract This study aimed to (1) create percentile curves and norms for the eight subtests of the Bruininks-Oseretsky Test of Motor Proficiency, 2nd edition (BOT-2) for 6- to 10-year-old Brazilian children and to (2) compare them to the values of the original test manual. To that, we tested a sample of 931 Brazilian children (477 girls, 454 boys) with ages between 6 to 10 with the BOT-2 assessment. The LMS method was used to generate the percentile curves and normative values, with the LMSchartmaker Pro software version 2.54. Results demonstrate that girls had significantly higher scores for the fine motor precision, fine motor integration, manual dexterity and balance subtests, while boys had significantly higher scores on upper-limb coordination, running speed and agility, and the strength subtests. The findings also indicated higher values for the Brazilian group in the subtests of bilateral coordination, running speed and agility, balance, and upper-limb coordination when compared to the North American normative sample. The percentile curves illustrate the increase in motor proficiency levels as age increases, with different trajectories for each subtest. Future studies should continue the investigation of cultural norms and appropriate assessments for the Brazilian population. Here, the creation of percentile curves and norms that are better suited for the Brazilian population can significantly help with assessment and intervention for motor development in distinct settings and with typical and atypical school-age children.


Resumo Este estudo objetivou (1) criar curvas e normas percentílicas para os oito subtestes do Teste de Proficiência Motora Bruininks-Oseretsky, 2ª edição (BOT-2) para crianças brasileiras de 6 a 10 anos e compará-las com os valores do manual de teste original. Para tanto, testamos uma amostra de 931 crianças brasileiras (477 meninas, 454 meninos) com idades entre 6 a 10 anos com a avaliação do BOT-2. O método LMS foi usado para gerar as curvas de percentil e valores normativos, utilizando o software LMSchartmaker Pro versão 2.54. Os resultados demonstram que as meninas tiveram escores significativamente mais altos nos subtestes de precisão motora fina, integração motora fina, destreza manual e equilíbrio, enquanto os meninos tiveram escores significativamente mais altos nos subtestes de coordenação de membros superiores, velocidade de corrida e agilidade e força. Os resultados também indicaram valores mais elevados para o grupo brasileiro nos subtestes de coordenação bilateral, corrida de velocidade e agilidade, equilíbrio e coordenação dos membros superiores quando comparados com a amostra normativa norte-americana. As curvas percentílicas ilustram o aumento nos níveis de proficiência motora à medida que a idade aumenta, com diferentes trajetórias para cada subteste. Estudos futuros devem continuar a investigação de normas culturais e avaliações apropriadas para a população brasileira. Neste estudo, a criação de curvas e normas percentílicas mais adequadas para a população brasileira pode ajudar significativamente na avaliação e intervenção para o desenvolvimento motor em diferentes contextos e com crianças em idade escolar típicas e atípicas.

18.
REVISA (Online) ; 9(2): 344-356, 2020.
Artigo em Português | LILACS | ID: biblio-1100156

RESUMO

Objetivo: realizar uma revisão integrativa sobre a associação entre o câncer de boca e a infecção pelo HPV. Método: foi realizada uma busca bibliográfica na base de dados científicos PubMed, utilizando descritores previamente selecionados. Dois revisores independentes avaliaram criticamente os resultados, obedecendo estritamente aos critérios de inclusão e exclusão definidos no protocolo do estudo. Além disso, a qualidade dos artigos foi avaliada considerando o Strengthening the reporting of observational studies in Epidemiology (STROBE). Resultados: Após a análise dos artigos, foram selecionados doze artigos, desses, sete mostraram ocorrência de HPV em pacientes com câncer de boca, e apenas dois estudos mostraram o HPV como possível etiologia para o câncer oral. Conclusão: não foi possível associar a presença do HPV à etiologia do câncer de boca, necessitando que mais estudos sejam realizados a fim de analisar melhor essa associaç


Objective: to carry out a integrative review on the association between oral cancer and HPV infection. Method: a bibliographic search was performed in the PubMed scientific database, using previously selected descriptors. Two independent reviewers critically evaluated the results, strictly obeying the inclusion and exclusion criteria defined in the study protocol. In addition, the quality of the articles was assessed considering the Strengthening the reporting of observational studies in Epidemiology (STROBE). Results: After analyzing the articles, twelve articles were selected, of these, seven showed the occurrence of HPV in patients with oral cancer, and only two studies showed HPV as a possible etiology for oral cancer. Conclusion: it was not possible to associate the presence of HPV with the etiology of oral cancer, requiring more studies to be carried out in order to better analyze this association


Objetivo: Objetivo: llevar a cabo una revisión integradora sobre la asociación entre el cáncer oral y la infección por VPH. Método: se realizó una búsqueda bibliográfica en la base de datos científica PubMed, utilizando descriptores previamente seleccionados. Dos revisores independientes evaluaron críticamente los resultados, obedeciendo estrictamente los criterios de inclusión y exclusión definidos en el protocolo del estudio. Además, se evaluó la calidad de los artículos considerando el Fortalecimiento de la notificación de estudios observacionales en Epidemiología (STROBE). Resultados: Después de analizar los artículos, se seleccionaron doce artículos, de estos, siete mostraron la presencia de HPV en pacientes con cáncer oral, y solo dos estudios mostraron el HPV como una posible etiología para el cáncer oral. La calidad del artículo se evaluó utilizando STROBE. Conclusión: no fue posible asociar la presencia de HPV con la etiología del cáncer oral, lo que requirió la realización de más estudios para analizar mejor esta asociación.


Assuntos
Neoplasias Bucais
19.
Arq. Inst. Biol ; 87: e0132020, 2020. tab
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1130089

RESUMO

This research aimed to evaluate the biological aspects and the feeding behavior of Aphis gossypii in watermelon cultivars submitted to silicon application. The experiment was conducted at the Institute of Education, Agriculture and Environment of the Federal University of Amazonas, Humaitá, Brazil. The experimental design was completely randomized in a 2×3 factorial (with and without silicon; cultivars Crimson Sweet, Fairfax and Charleston), with ten replications. The application of silicic acid (1%) was carried out directly on the substrate using dose equivalent to 1 ton SiO2·ha-1, 25 days after sowing. The rearing of aphids was kept in cucumber plants, cultivar Caipira. Insect biology tests were conducted to evaluate the duration of the prereproductive, reproductive and postreproductive periods, longevity, number of nymphs, and feeding behavior using the honeydew secretion technique. Analysis of variance was performed using the statistical program SISVAR and the means were compared by the F and Scott­Knott test (p ≤ 0.05). The silicon application to watermelon plants affects the reproduction and feeding of A. gossypii. The watermelon plants cultivar Crimson Sweet treated with silicon has high resistance to feeding by A. gossypii.(AU)


Nesta pesquisa objetivou-se avaliar os aspectos biológicos e o comportamento alimentar de Aphis gossypii em cultivares de melancia submetidas à aplicação de silício. O experimento foi conduzido no Instituto de Educação, Agricultura e Ambiente da Universidade Federal do Amazonas, Humaitá, Brasil. Utilizaram-se o delineamento experimental inteiramente ao acaso e o esquema fatorial 2×3 (sem silício e com silício; cultivares Crimson Sweet, Fairfax e Charleston), com dez repetições. A aplicação do ácido silícico (1%) foi realizada diretamente no substrato, com dose equivalente a 1 ton SiO2·ha-1, 25 dias após a semeadura. Os pulgões da criação foram mantidos em plantas de pepino, cultivar Caipira. Foram conduzidos ensaios de biologia do inseto para avaliação da duração dos períodos pré-reprodutivo, reprodutivo e pós-reprodutivo, longevidade, número de ninfas e comportamento alimentar por meio da técnica de secreção de honeydew. Realizou-se a análise de variância dos dados utilizando-se o programa estatístico SISVAR e as médias foram comparadas pelo teste de F e Scott­Knott (p ≤ 0,05). A aplicação de silício em plantas de melancia afeta a reprodução e a alimentação de A. gossypii. Plantas de melancia do cultivar Crimson Sweet tratadas com silício apresentam alta resistência à alimentação por A. gossypii.(AU)


Assuntos
Afídeos , Ácido Silícico , Gossypium , Citrullus , Controle de Pragas , Pragas da Agricultura , Cucurbitaceae , Comportamento Alimentar
20.
Fisioter. Mov. (Online) ; 33: e003346, 2020.
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1133879

RESUMO

Abstract Introduction: Professional training in physiotherapy still focuses primarily on rehabilitation; however, there is a need to ensure that these professionals are also equipped to work in primary care (PC), since it is the gateway to the healthcare system. Objective: Identify practices carried out in PC by undergraduate physiotherapy courses in Santa Catarina state, Brazil, from the perspective of professors. Method: Qualitative study with a multiple-case design. All eight undergraduate physiotherapy courses available in the state were included as cases in this study. Five physiotherapy professors also took part as key informants (KIs) Data were collected by document analysis of course pedagogical projects (CPPs) and a semi-structured interview regarding training practices conducted in primary care (PC) settings. Data were analyzed using thematic analysis. Results: Based on content analysis of the interviews, practices carried out in PC by the different courses include territorialization; specific group activities; home health care/home visits; postural education in school settings; interdisciplinary and multiprofessional experiences; interviews; waiting room activities; combined consultations and singular therapeutic projects. Conclusion: practices performed in primary care facilities by undergraduate physiotherapy courses in Santa Catarina state (SC), Brazil, are aimed at meeting primary care needs; however, teaching-service-community integration is essential in order to diversify these PC experiences throughout the training process and expand interdisciplinary and multiprofessional activities.


Resumo Introdução: A formação profissional em Fisioterapia ainda se dá muito centrada em práticas reabilitadoras, contudo, observa-se a necessidade de garantir no processo formativo práticas desenvolvidas na Atenção Básica (AB), visto que esta é a principal porta de entrada para o sistema de saúde. Objetivo: Desvelar as práticas realizadas na AB pelos cursos de graduação em Fisioterapia no estado de SC, segundo o olhar dos docentes. Método: Estudo de abordagem qualitativa, tendo como método o estudo de casos múltiplos. Oito cursos de graduação em Fisioterapia presentes no estado catarinense, constituíram-se nos casos desta pesquisa. Participaram do estudo cinco docentes fisioterapeutas como Informantes-Chave (IC). A coleta de dados se deu por meio de análise documental dos Projetos Pedagógicos dos Cursos (PPCs) e de entrevista semiestruturada quanto as práticas realizadas no contexto da AB. A análise de dados se deu mediante análise de conteúdo temática. Resultados: A partir da análise de conteúdo das entrevistas, entre as práticas realizadas na AB pelos cursos, estão a territorialização; ações em grupos específicos; atenção domiciliar/visita domiciliar; educação postural no contexto escolar; vivências interdisciplinares e multiprofissionais; entrevista; ações em sala de espera; consultas compartilhadas e projeto terapêutico singular. Conclusão: As práticas realizadas pelos cursos de graduação em Fisioterapia no estado de SC buscam atender as demandas da atenção básica, no entanto, há que se estabelecer a integração ensino-serviço-comunidade, a fim de potencializar a diversificação dessas vivências na AB ao longo do processo formativo, ainda ampliar as atividades interdisciplinares e multiprofissionais.

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