Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 26
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
Molecules ; 26(4)2021 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-33578817

RESUMO

Multidrug resistance (MDR) is the main challenge in the treatment of chronic myeloid leukemia (CML), and P-glycoprotein (P-gp) overexpression is an important mechanism involved in this resistance process. However, some compounds can selectively affect MDR cells, inducing collateral sensitivity (CS), which may be dependent on P-gp. The aim of this study was to investigate the effect of piperine, a phytochemical from black pepper, on CS induction in CML MDR cells, and the mechanisms involved. The results indicate that piperine induced CS, being more cytotoxic to K562-derived MDR cells (Lucena-1 and FEPS) than to K562, the parental CML cell. CS was confirmed by analysis of cell metabolic activity and viability, cell morphology and apoptosis. P-gp was partially required for CS induction. To investigate a P-gp independent mechanism, we analyzed the possibility that poly (ADP-ribose) polymerase-1 (PARP-1) could be involved in piperine cytotoxic effects. It was previously shown that only MDR FEPS cells present a high level of 24 kDa fragment of PARP-1, which could protect these cells against cell death. In the present study, piperine was able to decrease the 24 kDa fragment of PARP-1 in MDR FEPS cells. We conclude that piperine targets selectively MDR cells, inducing CS, through a mechanism that might be dependent or not on P-gp.

2.
Nutrition ; : 111045, 2020 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-33279359

RESUMO

OBJECTIVES: The aim of this study was to evaluate polyphenol intake in women with different classes of obesity and identify which are consumed more frequently and what the food sources are. METHODS: A cross-sectional study was conducted with 114 women with obesity. The study evaluated polyphenol intake via a 3-d food record using Phenol-Explorer. Anthropometric, biochemical, and dietetic variables were evaluated. RESULTS: The women's habitual food intake was low calorie and adequate in macronutrients. Mean polyphenol intake by the group was 573 ± 490, 614 ± 475, and 379 ± 25 mg/d for class I, class II, and class III obesity (P = 0.002), respectively. The most frequent food or beverage consumed by the group was coffee and caffeoylquinic acid, its phenolic compound. Fruits, vegetables, and nuts contributed the least to the intake of polyphenols. CONCLUSIONS: Although the diets of the study participants did include some food sources of polyphenols, they were not of sufficient quality to significantly contribute to a healthy diet; instead, they sometimes were foods may that contributed to weight gain. Women with class III obesity consumed the most calories; however, they had low fruit, vegetable, and whole foods intake.

3.
An Acad Bras Cienc ; 92(4): e20201181, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33295583

RESUMO

Leishmaniasis is caused by protozoan parasites belonging to the genus Leishmania and includes cutaneous, mucocutaneous and visceral clinical forms. Drugs currently available for leishmaniasis treatment present high toxicity, and development of parasite resistance. Plants constitute an important source of compounds with leishmanicidal potential. This study aimed to evaluate the anti-Leishmania amazonensis activity of the terpenoid fraction of Eugenia pruniformis leaves (TF-EpL). TF-EpL was active against the promastigote and intracellular amastigote forms of L. amazonensis with IC50(24h) value of 43.60µg/mL and 44.77µg/mL, respectively. TF-EpL altered the cell cycle of the parasite, increasing 2.32-fold the cells in the Sub-G0/G1 phase. TF-EpL also changed the ΔΨm and increased ROS and the number of annexin-V-PI positive promastigotes, which suggests incidental death. ß-sitosterol, ursolic acid, corosolic acid and asiatic acid were isolated from TF-EpL. The results showed the antileishmanial activity of E. pruniformis terpenoids and its potential for further studies as a source of new drugs for leishmaniasis.

4.
Molecules ; 25(15)2020 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-32752302

RESUMO

Phytochemicals and their metabolites are not considered essential nutrients in humans, although an increasing number of well-conducted studies are linking their higher intake with a lower incidence of non-communicable diseases, including cancer. This review summarizes the current findings concerning the molecular mechanisms of bioactive compounds from grapes and red wine and their metabolites on breast cancer-the most commonly occurring cancer in women-chemoprevention and treatment. Flavonoid compounds like flavonols, monomeric catechins, proanthocyanidins, anthocyanins, anthocyanidins and non-flavonoid phenolic compounds, such as resveratrol, as well as their metabolites, are discussed with respect to structure and metabolism/bioavailability. In addition, a broad discussion regarding in vitro, in vivo and clinical trials about the chemoprevention and therapy using these molecules is presented.

5.
Int J Mol Sci ; 21(15)2020 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-32721999

RESUMO

Breast cancer is the leading cause of cancer mortality in women worldwide. Conventional cancer treatment is costly and results in many side effects. Dietary bioactive compounds may be a potential source for breast cancer prevention and treatment. In this scenario, the aim of this study was to investigate the effects of the bioactive compounds resveratrol, curcumin and piperine (R-C-P) on MCF-7 breast cancer cells and to associate them to Glyoxalase 1 (GLO1) activity. The findings indicate that R-C-P exhibits cytotoxicity towards MCF-7 cells. R-C-P decreased mitochondrial membrane potential (ΔΨm) by 1.93-, 2.04- and 1.17-fold, respectively. Glutathione and N-acetylcysteine were able to reverse the cytotoxicity of the assessed bioactive compounds in MCF-7 cells. R-C-P reduced GLO1 activity by 1.36-, 1.92- and 1.31-fold, respectively. R-C-P in the presence of antimycin A led to 1.98-, 1.65- and 2.16-fold decreases in D-lactate levels after 2 h of treatment, respectively. Glyoxal and methylglyoxal presented cytotoxic effects on MCF-7 cells, with IC50 values of 2.8 and 2.7 mM and of 1.5 and 1.4 mM after 24 and 48 h of treatment, respectively. In conclusion, this study demonstrated that R-C-P results in cytotoxic effects in MCF-7 cells and that this outcome is associated with decreasing GLO1 activity and mitochondrial dysfunction.

6.
Biomed Res Int ; 2020: 5393041, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32149115

RESUMO

Resveratrol (Resv) offers health benefits in cancer and has been reported to modulate important enzymes of lipid metabolism. Studies of its effects on lipid composition in different subtypes of breast-cancer cells are scarce. Thus, we investigated the alterations in phospholipids (PL), fatty acids (FA), and lipid metabolism enzymes in two breast-cancer cell lines after Resv treatment. MCF-7 and MDA-MB-231 cells were treated with 80 and 200 µM of Resv, respectively, for 24 hours. We analyzed PL with radiolabeled inorganic phosphate (32Pi) by thin-layer chromatography, FA by gas chromatography-mass spectrometry, and lipid metabolism enzymes (DGAT2, FAS, ρACCß, pAMPKα, and AMPK) by Western blot. Resv treated MDA-MB-231 phospholipids showed a reduction in phosphatidylcholine (63%) and phosphatidylethanolamine (35%). We observed an increase in eicosapentaenoic acid (EPA) (73%) and docosahexaenoic acid (DHA) (65%) in MCF-7 cells after Resv treatment. Interestingly, the same treatment caused 50% and 90% increases in EPA and DHA, respectively, in MDA-MB-231 cells. In MCF-7 cells, Resv increased the expression of ρACCß (3.3-fold) and AMPKα/ρAMPKα (1.5-fold) and in MDA-MB-231 cells it inhibited the expression of ρACCß (111.8-fold) and AMPKα/ρAMPKα (1.2 fold). Our results show that Resv modified PL and saturated and unsaturated FA especially in MDA-MB-231 cells, and open new perspectives to the understanding of the reported anticancer effect of Resv on these cells.


Assuntos
Neoplasias da Mama/tratamento farmacológico , Metabolismo dos Lipídeos/efeitos dos fármacos , Resveratrol/farmacologia , Proteínas Quinases Ativadas por AMP/metabolismo , Neoplasias da Mama/metabolismo , Linhagem Celular Tumoral/efeitos dos fármacos , Linhagem Celular Tumoral/metabolismo , Ácidos Docosa-Hexaenoicos/metabolismo , Ácido Eicosapentaenoico/análogos & derivados , Ácidos Graxos/metabolismo , Ácidos Graxos Insaturados , Feminino , Humanos , Lipídeos/análise , Células MCF-7 , Fosfatidilcolinas/metabolismo , Fosfatidiletanolaminas/metabolismo , Fosfolipídeos/metabolismo , Resveratrol/uso terapêutico
7.
Sci Rep ; 9(1): 13960, 2019 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-31562347

RESUMO

Flotillin-1 and flotillin-2 are highly conserved proteins that localize into cholesterol-rich microdomains in cellular membranes. Flotillins are closely related to the occurrence and development of various types of human cancers. Flotillin-1 is highly expressed in breast cancer, and the high expression level of flotillin-1 is significantly correlated with poorer patient survival. Here we studied the relationship between the formation of lipid rafts and the expression of flotillins and lipids in human breast cancer cells. We used the polyphenol compound resveratrol to alter the structure and function of the plasma membrane. Our data revealed an increase in fatty acids in MCF-7 and MDA-MB-231 cells upon resveratrol treatment. Interestingly, we also found an increase in the expression of both flotillin-1 and flotillin-2 in breast tumor cells after treatment. Resveratrol also induced changes in the pattern of flotillin distribution among detergent-resistant lipid rafts fractions in both cell lines and induced the nuclear translocation of flotillin-2. Since resveratrol has been pointed out as a putative cancer therapy agent, our results could have an impact on the understanding of the effects of resveratrol in tumor cells.


Assuntos
Antioxidantes/farmacologia , Membrana Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Ácidos Graxos/metabolismo , Proteínas de Membrana/metabolismo , Resveratrol/farmacologia , Neoplasias da Mama/metabolismo , Membrana Celular/metabolismo , Humanos , Células MCF-7 , Microdomínios da Membrana/efeitos dos fármacos , Microdomínios da Membrana/metabolismo
8.
Molecules ; 22(6)2017 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-28629161

RESUMO

Increasing epidemiological and experimental evidence has demonstrated an inverse relationship between the consumption of plant foods and the incidence of chronic diseases, including cancer. Microcomponents that are naturally present in such foods, especially polyphenols, are responsible for the benefits to human health. Resveratrol is a diet-derived cancer chemopreventive agent with high therapeutic potential, as demonstrated by different authors. The aim of this review is to collect and present recent evidence from the literature regarding resveratrol and its effects on cancer prevention, molecular signaling (especially regarding the involvement of p53 protein), and therapeutic perspectives with an emphasis on clinical trial results to date.


Assuntos
Antineoplásicos Fitogênicos/uso terapêutico , Quimioprevenção , Neoplasias/tratamento farmacológico , Neoplasias/prevenção & controle , Estilbenos/uso terapêutico , Animais , Antineoplásicos Fitogênicos/farmacologia , Disponibilidade Biológica , Biomarcadores Tumorais , Ensaios Clínicos como Assunto , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Neoplasias/genética , Neoplasias/metabolismo , Avaliação de Resultados em Cuidados de Saúde , Resveratrol , Transdução de Sinais/efeitos dos fármacos , Estilbenos/farmacologia , Proteína Supressora de Tumor p53/antagonistas & inibidores , Proteína Supressora de Tumor p53/genética , Proteína Supressora de Tumor p53/metabolismo
9.
J Agric Food Chem ; 62(6): 1283-93, 2014 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-24377746

RESUMO

Soybeans have several functional properties due to their composition and may exert beneficial health effects that are attributed to proteins and their derivative peptides. The present study aimed to analyze the protein profiles of four new conventional soybean seeds (BRS 257, BRS 258, BRS 267, and Embrapa 48) with the use of proteomic tools. Two-dimensional (2D) and one-dimensional (1D) gel electrophoreses were performed, followed by MALDI-TOF/TOF and ESI-Q-TOF mass spectrometry analyses, respectively. These two different experimental approaches allowed the identification of 117 proteins from 1D gels and 46 differentially expressed protein spots in 2D gels. BRS 267 showed the greatest diversity of identified spots in the 2D gel analyses. In the 1D gels, the major groups were storage (25-40%) and lipid metabolism (11-25%) proteins. The differences in protein composition between cultivars could indicate functional and nutritional differences and could direct the development of new cultivars.


Assuntos
Proteômica , Sementes/química , Proteínas de Soja/análise , Soja/química , Eletroforese em Gel de Poliacrilamida , Especificidade da Espécie , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
10.
PLoS One ; 7(11): e48746, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23152798

RESUMO

Resveratrol is a promising chemopreventive agent that mediates many cellular targets involved in cancer signaling pathways. p53 has been suggested to play a role in the anticancer properties of resveratrol. We investigated resveratrol-induced cytotoxicity in H1299 cells, which are non-small lung cancer cells that have a partial deletion of the gene that encodes the p53 protein. The results for H1299 cells were compared with those for three cell lines that constitutively express wild-type p53: breast cancer MCF-7, adenocarcinomic alveolar basal epithelia A549 and non-small lung cancer H460. Cell viability assays revealed that resveratrol reduced the viability of all four of these cell lines in a dose- and time-dependent manner. MCF-7, A549 and H460 cells were more sensitive to resveratrol than were H1299 cells when exposed to the drug for 24 h at concentrations above 100 µM. Resveratrol also increased the p53 protein levels in MCF-7 cells without altering the p53 mRNA levels, suggesting a post-translational modulation of the protein. The resveratrol-induced cytotoxicity in these cells was partially mediated by p53 and involved the activation of caspases 9 and 7 and the cleavage of PARP. In H1299 cells, resveratrol-induced cytotoxicity was less pronounced and (in contrast to MCF-7 cells) cell death was not accompanied by caspase activation. These findings are consistent with the observation that MCF-7 cells were positively labeled by TUNEL following exposure to 100 µM resveratrol whereas H1299 cells under similar conditions were not labeled by TUNEL. The transient transfection of a wild-type p53-GFP gene caused H1299 cells to become more responsive to the pro-apoptotic properties of resveratrol, similarly to findings in the p53-positive MCF-7 cells. Our results suggest a possible therapeutic strategy based on the use of resveratrol for the treatment of tumors that are typically unresponsive to conventional therapies because of the loss of normal p53 function.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Apoptose/efeitos dos fármacos , Apoptose/genética , Estilbenos/farmacologia , Proteína Supressora de Tumor p53/genética , Antineoplásicos Fitogênicos/toxicidade , Neoplasias da Mama/genética , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/genética , Relação Dose-Resposta a Droga , Feminino , Humanos , Neoplasias Pulmonares/genética , Células MCF-7 , Masculino , Resveratrol , Estilbenos/toxicidade , Ativação Transcricional/efeitos dos fármacos , Transfecção , Proteína Supressora de Tumor p53/metabolismo
11.
J Agric Food Chem ; 60(19): 4826-32, 2012 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-22509740

RESUMO

Consumption of plant food rich meals, such as feijoada, a traditional meal in Brazil, is associated with the reduction of chronic disease. The objectives of this study were to determine phytochemical content and antioxidant activity by chemical and cellular antioxidant assays (CAA) of feijoada with or without in vitro digestion. Feijoada showed no difference in phenolics and flavonoids after digestion. Bound and residue contributions to total phenolics were 20.9% and 32.2%, respectively, suggesting that phenolics reach the colon after intake. Flavonoids in residue and bound fractions represented 50% of total flavonoids. Antioxidant activity of feijoada without digestion was higher than that with digestion; however, it showed lower antiproliferative activity and CAA. Feijoada with in vitro digestion also yielded phenolics with higher CAA. Analyses of whole meals should be used to evaluate phytochemicals present in food mixtures consumed, especially with digestion models coupled with CAA resulting in information similar to those in physiological conditions.


Assuntos
Antioxidantes/química , Células/efeitos dos fármacos , Dieta Vegetariana , Digestão , Grão Comestível/química , Extratos Vegetais/química , Antioxidantes/farmacologia , Brasil , Proliferação de Células/efeitos dos fármacos , Células/metabolismo , Flavonoides/química , Flavonoides/farmacologia , Células Hep G2 , Humanos , Modelos Biológicos , Oxirredução , Fenóis/química , Fenóis/farmacologia , Extratos Vegetais/farmacologia
12.
J Cell Biochem ; 113(8): 2586-96, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22415970

RESUMO

Melphalan (MEL) is a chemotherapeutic agent used in breast cancer therapy; however, MEL's side effects limit its clinical applications. In the last 20 years, resveratrol (RSV), a polyphenol found in grape skins, has been proposed to reduce the risk of cancer development. The aim of this study was to investigate whether RSV would be able to enhance the antitumor effects of MEL in MCF-7 and MDA-MB-231 cells. RSV potentiated the cytotoxic effects of MEL in human breast cancer cells. This finding was related to the ability of RSV to sensitize MCF-7 cells to MEL-induced apoptosis. The sensitization by RSV involved the enhancement of p53 levels, the decrease of procaspase 8 and the activation of caspases 7 and 9. Another proposed mechanism for the chemosensitization effect of MCF-7 cells to MEL by RSV was the cell cycle arrest in the S phase. The treatment with RSV or MEL increased the levels of p-Chk2. The increase became pronounced in the combined treatments of the compounds. The expression of cyclin A was decreased by treatment with RSV and by the combination of RSV with MEL. While the levels of cyclin dependent kinase 2 (CDK2) remained unchanged by treatments, its active form (Thr(160) -phosphorylated CDK2) was decreased by treatment with RSV and by the combination of RSV with MEL. The activity of CDK7, kinase that phosphorylates CDK2 at Thr(160), was inhibited by RSV and by the combination of RSV with MEL. These results indicate that RSV could be used as an adjuvant agent during breast cancer therapy with MEL.


Assuntos
Melfalan/farmacologia , Estilbenos/farmacologia , Apoptose/efeitos dos fármacos , Western Blotting , Neoplasias da Mama/metabolismo , Ciclo Celular/efeitos dos fármacos , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Feminino , Humanos , Imunoprecipitação , Resveratrol
13.
Rev. nutr ; 22(2): 257-269, mar.-abr. 2009. ilus, tab
Artigo em Português | LILACS | ID: lil-517451

RESUMO

O chá é uma bebida amplamente utilizada, perdendo apenas para a água como a bebida mais consumida no mundo. O chá verde é rico em polifenóis, principalmente catequinas. Entre uma variedade de efeitos benéficos à saúde atribuídos ao consumo do chá verde, grande atenção tem sido focalizada no seu efeito na redução da gordura corporal. Este estudo tem como objetivo apresentar uma descrição dos estudos com o chá verde e/ou seus compostos bioativos relacionados à biologia celular, estudos experimentais e epidemiológicos associados ao metabolismo lipídico e à redução da gordura corporal. Galato de epigalocatequina é o principal composto bioativo presente no chá verde e seus efeitos anti-obesidade estão sendo investigados. Tais efeitos estão associados a diversos mecanismos bioquímicos e fisiológicos, dentre eles podem-se destacar a estimulação do metabolismo lipídico pela combinação da ingestão de catequinas e a prática de exercícios físicos regulares. Apesar do efeito promissor do chá verde e seus compostos bioativos no tratamento da obesidade, estudos clínicos controlados devem ser conduzidos. Finalmente, um plano alimentar adequado associado à prática regular de atividade física constitui a principal ferramenta para a prevenção da obesidade e de suas comorbidades.


The use of tea is widespread, second only to water in worldwide consumption. Green tea is rich in polyphenols, mainly catechins. Among a variety of beneficial health effects attributed to the consumption of the green tea, much attention has been given to its ability to reduce body fat. This study has the objective of presenting a description of the studies of green tea and/or its bioactive compounds related to cell biology and of experimental and epidemiological studies associated with lipid metabolism and the reduction of body fat. Epigallocatechin gallate is the main bioactive compound present in green tea and its anti-obesity effects are being investigated. Such effects are associated with several biochemical and physiological mechanisms and among them the following stands out: stimulation of lipid metabolism by combining catechin intake with regular physical activity. In spite of the promising effect of green tea and its bioactive compounds on the treatment of obesity, there is a need for controlled clinical trials. Finally, a proper diet associated with regular physical activity is the key to prevent obesity and its comorbidities.


Assuntos
Camellia sinensis/fisiologia , Alimento Funcional , Metabolismo dos Lipídeos/fisiologia , Obesidade/prevenção & controle
14.
Rev. saúde pública ; 43(2): 211-218, abr. 2009. tab
Artigo em Inglês, Português | LILACS | ID: lil-507813

RESUMO

OBJETIVO: Estimar a disponibilidade de polifenóis totais em frutas e hortaliças consumidas no Brasil segundo macrorregião e identificar os principais alimentos-fonte que fazem parte do hábito alimentar nacional. MÉTODOS: O conteúdo de polifenóis foi determinado pelo método Folin-Ciocalteu e sua disponibilidade foi estimada com base na Pesquisa de Orçamentos Familiares 2002/2003. Foram escolhidos 12 alimentos de maior consumo, sendo seis "frutas tropicais" e seis "hortaliças folhosas e florais", "hortaliças frutosas" e "hortaliças tuberosas". A determinação de polifenóis foi realizada em três experimentos independentes, cada um em duplicata. A disponibilidade nacional de polifenóis foi estimada por grama de peso fresco de cada vegetal analisado. A ingestão diária per capita no Brasil e regiões foi calculada como sendo o aporte diário de polifenóis fornecido pelo consumo dos 12 alimentos analisados. RESULTADOS: O teor de polifenóis nos alimentos variou de 15,35 a 214,84mg EAG/100g peso fresco. A disponibilidade nacional, com base na quantidade, em kg, adquirida anualmente no Brasil foi de 48,3mg/dia, tendo a região Sudeste e a região Centro-Oeste os maiores e menores valores, respectivamente. A banana foi a principal fonte de polifenóis consumida no Brasil, variando conforme macrorregião. CONCLUSÕES: A estimativa de disponibilidade de polifenóis no Brasil encontrada foi semelhante à de outros países. Diferenças observadas entre as macrorregiões geográficas podem estar diretamente relacionadas às diferenças culturais de cada região. Apesar de não haver uma quantidade recomendada para o consumo de polifenóis, a adoção da recomendação diária de frutas e hortaliças representa um aumento de 16 vezes na disponibilidade nacional de polifenóis, demonstrando a relação entre o consumo destes grupos alimentares com a ingestão de compostos bioativos benéficos à saúde.


OBJECTIVE: To estimate total polyphenol availability in fruits and vegetables commonly consumed in Brazil and its regions, and to identify the main food sources that constitute food habits in this country. METHODS: Total polyphenols were determined by the Folin-Ciocalteu method and the availability estimated according to the Pesquisa de Orçamentos Familiares 2002/ 2003 (2002/2003 Family Budget Survey). Twelve highly consumed food items were chosen, of which six were "tropical fruits" and six were vegetables under the categories of "leafy and flower vegetables", "fruit vegetables" and "tuberous vegetables". Polyphenol quantification was performed with three independent experiments, each one in duplicate. The national polyphenol availability was estimated in grams per fresh weight of each analyzed food. Daily per capita availability in Brazil and its regions was calculated using the amount of polyphenol provided by the consumption of the 12 foods analyzed. RESULTS: Polyphenol contents of foods varied from 15.35 to 214.84 mg GAE/ 100 g of fresh weight. Polyphenol availability in Brazil, based on the amount in kilograms that is annually acquired in Brazil, of the 12 selected foods was 48.3 mg/ day, and the Southeast and Central-West regions had the highest and lowest values, respectively. Banana was the main polyphenol source consumed in Brazil, even though this pattern varied among regions. CONCLUSIONS: The estimated daily polyphenol availability in Brazil was similar to other countries. Differences observed among regions could be directly related to distinct cultural habits. Although there is no recommended daily availability of polyphenols, consumption of the recommended daily amount of fruits and vegetables can increase the availability of polyphenols 16 times, showing a clear relationship between the consumption of these food groups and the availability of beneficial bioactive compounds.


OBJETIVO: Estimar la disponibilidad de polifenoles totales en frutas y hortalizas consumidas en Brasil según macro-región e identificar los principales alimentos fuente que forman parte del hábito alimenticio nacional. MÉTODOS: El contenido de polifenoles fue determinado por el método Folin-Ciocalteu y su disponibilidad fue estimada con base en la Pesquisa de Presupuesto Familiares 2002/2003. Fueron escogidos 12 alimentos de mayor consumo, siendo seis "frutas tropicales" y seis "hortalizas de hojas y florales", "hortalizas frutales" y "hortalizas de tubérculos". La determinación de polifenoles fue realizada en tres experimentos independientes, cada uno por duplicado. La disponibilidad nacional de polifenoles fue estimada por gramo de peso fresco de cada vegetal analizado. La ingestión diaria per capita en Brasil y regiones fue calculada como el aporte diario suministrado por el consumo de los 12 alimentos analizados. RESULTADOS: La proporción de polifenoles en los alimentos varió de 15,35 a 214,84 mg EAG/100 g peso fresco. La disponibilidad nacional, con base en la cantidad, en kg, adquirida anualmente en Brasil fue de 48,3 mg/día, teniendo la región Sureste y la región Centro-oeste los mayores y menores valores, respectivamente. La banana fue la principal fuente de polifenoles consumida en Brasil, variando conforme macro-región. CONCLUSIONES: La estimación de disponibilidad de polifenoles en Brasil encontrada fue semejante a la de otros países. Diferencias observadas entre macro-regiones geográficas pueden estar directamente relacionadas a las diferencias culturales de cada región. A pesar de no haber una cantidad recomendada para el consumo de polifenoles, la adopción de la recomendación diaria de frutas y hortalizas representa un aumento de 16 veces en la disponibilidad nacional de polifenoles, demostrando la relación entre el consumo de estos grupos de alimentos con la ingestión de compuestos bioactivos beneficiosos para la salud.


Assuntos
Humanos , Comportamento Alimentar , Flavonoides/análise , Frutas/química , Fenóis/análise , Verduras/química , Brasil
15.
Rev Saude Publica ; 43(2): 211-8, 2009 Apr.
Artigo em Português | MEDLINE | ID: mdl-19225692

RESUMO

OBJECTIVE: To estimate total polyphenol availability in fruits and vegetables commonly consumed in Brazil and its regions, and to identify the main food sources that constitute food habits in this country. METHODS: Total polyphenols were determined by the Folin-Ciocalteu method and the availability estimated according to the Pesquisa de Orçamentos Familiares 2002/ 2003 (2002/2003 Family Budget Survey). Twelve highly consumed food items were chosen, of which six were 'tropical fruits' and six were vegetables under the categories of 'leafy and flower vegetables', 'fruit vegetables' and 'tuberous vegetables'. Polyphenol quantification was performed with three independent experiments, each one in duplicate. The national polyphenol availability was estimated in grams per fresh weight of each analyzed food. Daily per capita availability in Brazil and its regions was calculated using the amount of polyphenol provided by the consumption of the 12 foods analyzed. RESULTS: Polyphenol contents of foods varied from 15.35 to 214.84 mg GAE/ 100 g of fresh weight. Polyphenol availability in Brazil, based on the amount in kilograms that is annually acquired in Brazil, of the 12 selected foods was 48.3 mg/ day, and the Southeast and Central-West regions had the highest and lowest values, respectively. Banana was the main polyphenol source consumed in Brazil, even though this pattern varied among regions. CONCLUSIONS: The estimated daily polyphenol availability in Brazil was similar to other countries. Differences observed among regions could be directly related to distinct cultural habits. Although there is no recommended daily availability of polyphenols, consumption of the recommended daily amount of fruits and vegetables can increase the availability of polyphenols 16 times, showing a clear relationship between the consumption of these food groups and the availability of beneficial bioactive compounds.


Assuntos
Comportamento Alimentar , Flavonoides/análise , Frutas/química , Fenóis/análise , Verduras/química , Brasil , Humanos , Polifenóis
16.
Rev. nutr ; 21(6): 757-766, nov.-dez. 2008. ilus
Artigo em Português | LILACS | ID: lil-509608

RESUMO

Após seqüenciamento do genoma humano, os estudos genômicos têm se voltado à elucidação das funções de todos os genes, bem como à caracterização de suas interações com fatores ambientais. A nutrigenômica surgiu no contexto do pós-genoma humano e é considerada área-chave para a nutrição nesta década. Seu foco de estudo baseia-se na interação gene-nutriente. Esta ciência recente tem como objetivo principal o estabelecimento de dietas personalizadas, com base no genótipo, para a promoção da saúde e a redução do risco de doenças crônicas não transmissíveis como as cardiovasculares, o câncer, o diabetes, entre outras. Nesse contexto, é fundamental a aplicação na área de nutrição das ferramentas de genômica funcional para análise do transcritoma (transcritômica), do proteoma (proteômica) e do metaboloma (metabolômica). As aplicabilidades dessas metodologias em estudos nutricionais parecem ilimitadas, pois podem ser conduzidas em cultura de células, modelos de experimentação em animais, estudos pré-clinicos e clínicos. Tais técnicas apresentam potencial para identificar biomarcadores que respondem especificamente a um determinado nutriente ou composto bioativo dos alimentos e para estabelecer as melhores recomendações dietéticas individuais para redução do risco das doenças crônicas não transmissíveis e promoção da saúde.


After sequencing the human genome, genomic studies have been focusing on elucidating the function of all genes, as well as characterizing their interactions with environmental factors. Nutrigenomics emerged in the pos-genome era and is considered a key-area for nutrition in the present decade. Its research focus is nutrient-gene interaction. The main objective of this recent science is to establish personalized genotype-based diets that promote health and reduce the risk of non-communicable chronic diseases such as cardiovascular diseases, cancer, diabetes and others. In this context, it is essential to use functional genomic tools to analyze the transcriptome (transcriptomics), proteome (proteomics) and metabolome (metabolomics) in the field of nutrition. The applicabilities of such methodologies in nutritional studies seem unlimited since they can be conducted in cell cultures, animal models and pre-clinical and clinical studies. Such techniques may allow one to identify biomarkers that respond specifically to a certain dietary nutrient or bioactive compound and to establish the best individual dietary advice to reduce the risk of non-communicable chronic diseases and promote health.


Assuntos
Ciências da Nutrição , Nutrigenômica/métodos , Proteômica/métodos
17.
Biol Res ; 41(3): 317-30, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-19399344

RESUMO

Lactating Wistar rats were fed a liquid diet containing either ethanol [ethanol-fed group (EFG)] or an isocaloric amount of carbohydrate [pair-fed group (PFG)] from day 1 postpartum up to day 14 of lactation, to investigate micro/macronutrient milk composition and the mineral status of pups. EFG presented a reduction of daily milk production and milk composition was significantly higher in protein and lower in carbohydrate, while the lipid content was similar to that of PFG. When compared to PFG, the milk of EFG had a decreased proportion of C22:6 n-3 fatty acid and an increase in medium-chain fatty acids and of several minerals. Pups of EFG showed reduced growth and a lower concentration of Cu and Sr in plasma and lower concentrations of Ca, P and Cl, and higher concentrations of Cd in the brain. We conclude that maternal EtOH intake greatly impairs lactational performance and modifies the mineral status of pups.


Assuntos
Animais Lactentes/crescimento & desenvolvimento , Química Encefálica/efeitos dos fármacos , Etanol/farmacologia , Fígado/química , Leite/efeitos dos fármacos , Minerais/análise , Animais , Animais Recém-Nascidos , Peso Corporal/efeitos dos fármacos , Carboidratos da Dieta/administração & dosagem , Etanol/administração & dosagem , Feminino , Fígado/efeitos dos fármacos , Leite/química , Ratos , Ratos Wistar , Fatores de Tempo
18.
Biol. Res ; 41(3): 317-330, 2008. tab, graf
Artigo em Inglês | LILACS | ID: lil-511921

RESUMO

Lactating Wistar rats were fed a liquid diet containing either ethanol [ethanol-fed group (EFG)] or an isocaloric amount of carbohydrate [pair-fed group (PFG)] from day 1 postpartum up to day 14 of lactation, to investigate micro/macronutrient milk composition and the mineral status of pups. EFG presented a reduction of daily milk production and milk composition was significantly higher in protein and lower in carbohydrate, while the lipid content was similar to that of PFG. When compared to PFG, the milk of EFG had a decreased proportion of C22:6 n-3 fatty acid and an increase in medium-chain fatty acids and of several minerals. Pups of EFG showed reduced growth and a lower concentration of Cu and Sr in plasma and lower concentrations of Ca, P and Cl, and higher concentrations of Cd in the brain. We conclude that maternal EtOH intake greatly impairs lactational performance and modifies the mineral status of pups.


Assuntos
Animais , Feminino , Ratos , Animais Lactentes/crescimento & desenvolvimento , Química Encefálica/efeitos dos fármacos , Etanol/farmacologia , Fígado/química , Leite/efeitos dos fármacos , Minerais/análise , Animais Recém-Nascidos , Peso Corporal/efeitos dos fármacos , Carboidratos da Dieta/administração & dosagem , Etanol/administração & dosagem , Fígado/efeitos dos fármacos , Leite/química , Ratos Wistar , Fatores de Tempo
19.
Acta Trop ; 103(3): 222-30, 2007 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-17686447

RESUMO

Retinoic acid and insect juvenile hormone (JH) are structurally related terpenoids which are widespread in nature and are involved in many biological events such as morphogenesis, embryogenesis and cellular differentiation. Here, we investigated the effects of the retinoids 9-cis retinoic acid (9cisRA), all trans retinol (atROH), all trans retinoic acid (atRA) and the juvenoids methoprene (Met) and JH injection on moult and on phenoloxidase activity in the blood-sucking insect Rhodnius prolixus. Overall, we observed that injection of retinoids or juvenoids (120 pmols) in the hemocoel of 4th instar nymphs reduced the percentage of insects which appeared normal in morphology upon moult. Noteworthy, insects exposed to 9cisRA or JH underwent profound morphological changes upon moult, generating abnormal 5th instar nymphs and also markedly increased the death of insects during the moulting process. In addition, reduction in the percentage of insects that moult without any morphological alteration, induced by retinoids or juvenoids treatment, was negatively correlated with insects that both display abnormal moult and those that die during moult. Hemolymphatic phenoloxidase activity in adult male insects injected with 9cisRA, Met and JH were significantly reduced after a bacterial challenge. Together, these results indicate that not only juvenoids but also retinoids play an important role on morphogenesis and on immune response in R. prolixus, suggesting that the molecular mechanisms involved in these events recognize the terpenoid backbone as an important structural determinant in insects.


Assuntos
Hormônios Juvenis/farmacologia , Metoprene/farmacologia , Muda/efeitos dos fármacos , Monofenol Mono-Oxigenase/efeitos dos fármacos , Retinoides/farmacologia , Rhodnius/efeitos dos fármacos , Animais , Hormônios Juvenis/química , Metoprene/química , Monofenol Mono-Oxigenase/metabolismo , Retinoides/química , Rhodnius/enzimologia , Rhodnius/crescimento & desenvolvimento
20.
Bol. Centro Pesqui. Process. Aliment ; 25(2): 235-246, jul.-ago. 2007. tab, graf
Artigo em Português | LILACS | ID: lil-481530

RESUMO

Este estudo teve como objetivo avaliar as estabilidades química, físico-química e microbiólogica de suco de laranja cv. "Pêra", mmantido em temperatura ambiente, estocado sob refrigeração ou sob congelamento durante diferentes períodos. Determinaram-se os teores de ácido ascórbico e de sólidos solúveis totais, a acidez total titulável e o pH dos sucos. Foram realizadas, também, ascontagens de bactérias heterotróficas aeróbicas mesófilas, fungos miceliais e leveduras, bactérias láticas, coliformes totais e fecais e Salmonella. Os teores de ácido ascórbico e de sólidos solúveis totais dos sucos permaneceram estáveis durante o período de estocagem nas condições estudadas. O mesmo foi observado para acidez total titulável e pH, exceto para os sucos congelados em que houve aqlteração desses parãmetros a partir de 120 dias de estocagemm. Não foram encontrados Salmonella spp. e Coliformes totais e fecais nas amostras estudadas. A contagem inicial das bactérias heterotróficas aeróbicas mesófilas, fungos miceliais e leveduras e bactérias láticas foi de aproximadamente 3 Log UFC/mL. No suco mantido em temperatura ambiente durante 24 horas observou-se aumento de 1,5, 2,3 e 3,4 Log UFC/mL na contagem de bactérias heterotróficas aeróbicas mesófilas, fungos miceliais e leveduras e bactériasláticas, respectivamente. Nos sucos refrigerados, a contagem microbiana não excedeu a inicial até o quarto dia. Nos sucos congelados, a carga microbiana permaneceu inferior à inicial durante 180 dias de estocagem. De acordo com os resultados obtidos, o suco de laranja in natura preserva sua qualidade quando estocado sob refrigeração durante 48 horas ou sob congelamento durante 120 dias.


Assuntos
Citrus sinensis , Microbiologia de Alimentos , Conservação de Alimentos , Tecnologia de Alimentos , Frutas
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...