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2.
Gene ; 715: 143991, 2019 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-31357023

RESUMO

BACKGROUND: Asthma is a complex disease with worldwide public health relevance, is related to environmental causes and a genetic predisposition. The chromosomal 17q12-21 locus has been consistently demonstrated to be associated with asthma risk. The effects of variants in the 17q12-21 locus on childhood asthma were first identified in a genome wide- association study. Since that time, those findings have been replicated in different populations but not in South American populations. OBJECTIVE: This study aimed to investigate the role of variants in the 17q12-21 locus on asthma in a sample of Brazilian children. METHODS: This was a cross-sectional study conducted on a cohort of 1247 children. These analyses used 50 Single Nucleotide Variants (SNVs) in the 17q12-21 locus were genotyped as part of a genome wide association study (GWAS). RESULTS: Four SNVs (rs4065275, rs12603332, rs73985228 and rs77777702) were associated with childhood asthma. The rs73985228 exhibited the strongest association across the different genetic models (OR, 95%CI 2.8, 1.44-3.21, p < 0.01). In an analysis that was stratified by atopy, two SNVs (rs73985228 and rs2715555) were found to be associated with atopic and non-atopic asthma. For the first time, we observed a significant interaction with seropositivity for the Varicella zoster virus (for rs4065275, p = 0.02, and for rs12603332, p = 0.04); i.e., the association was found in those who were seropositive but not in those who were seronegative for this virus. CONCLUSIONS: We confirmed the associations of variants in the 17q12-21 locus with atopic and non-atopic asthma and identified an interaction with seropositivity for the Varicella zoster virus.


Assuntos
Asma/genética , Cromossomos Humanos Par 17/genética , Predisposição Genética para Doença , Herpesvirus Humano 3 , Polimorfismo de Nucleotídeo Único , Infecção pelo Vírus da Varicela-Zoster/genética , Asma/virologia , Criança , Pré-Escolar , Estudos Transversais , Feminino , Loci Gênicos , Estudo de Associação Genômica Ampla , Humanos , Masculino
3.
Artigo em Inglês | MEDLINE | ID: mdl-31126515

RESUMO

BACKGROUND: Asthma is a chronic disease of the airways and its most common phenotype is characterized by a T2 type response with IgE production and inflammatory mediators in response to common allergens. Cysteinyl leukotrienes (CysLTs), LTC4, LTD4 and LTE4, are mediators known to possess important proinflammatory action. CysLTs can bind to the Cysteinyl leukotriene receptor type 2 (CysLTR2) and activate an inflammatory. Polymorphisms in CysLTR2 have been associated with asthma and atopy, although the mechanism is not clear. OBJECTIVE: To evaluate the association between genetic polymorphisms in CYSLTR2 with asthma phenotypes, atopy markers and helminth infection. METHODS: Genotyping was performed using a panel Illumina and carried out in 1245 participants of SCAALA program (Social Change, Asthma, Allergy in Latin American). Logistic regressions for asthma, helminth infections (Trichuris trichiura and Ascaris lumbricoides) and allergy markers (skin tests and IgE production) were performed using PLINK 1.9 software adjusted for sex, age, helminth infection and ancestry markers. RESULTS: The G allele of rs1323556 was negatively associated with asthma in the additive model (OR 0.74, 95% CI 0.59-0.93) and in the dominant model (OR 0.71, 95% CI 0.53-0.74). The G allele of rs1575464 was also negatively associated with asthma in two genetic models, additive (OR 0.77, 95% CI 0.62-0.96) and dominant (OR 0.73, 95% CI 0.55-0.97). The G allele of rs61735175 was positively associated with asthma severity in the additive model (OR 1.72, 95% CI 1.07-2.77) and in the dominant model (OR 1.77, 95% CI 1.09-2.85). Five SNVs were associated with atopy markers and four SNVs were associated with helminth infections. CONCLUSION: Polymorphisms in the CYSLTR2 gene are associated with asthma, atopy markers and helminth infection in Brazilian individuals, which may lead to protection or risk for such conditions, however, more studies are needed to evaluate the functional of this variants here in described.

4.
Mediators Inflamm ; 2019: 6758159, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31011284

RESUMO

This study aimed at evaluating the transcriptional profile of apoptosis-related genes after in vitro stimulation of peripheral blood mononuclear cells (PBMCs) derived from individuals with periodontitis (P) and healthy nonperiodontitis (NP) control subjects with P. gingivalis HmuY protein. PBMCs from the P and NP groups were stimulated with HmuY P. gingivalis protein, and the expression of genes related to apoptosis was assessed by custom real-time polymerase chain reaction array (Custom RT2 PCR Array). Compared with the NP group, the P group showed low relative levels of apoptosis-related gene expression, downregulated for FAS, FAS ligand, TNFSF10 (TRAIL), BAK1, CASP9, and APAF1 after P. gingivalis HmuY protein stimulation. Furthermore, the P group exhibited low levels of relative gene expression, downregulated for CASP7 when the cells were not stimulated. Our data suggest that P. gingivalis HmuY protein might participate differently in the modulation of the intrinsic and extrinsic apoptosis pathways.


Assuntos
Apoptose/fisiologia , Proteínas de Bactérias/metabolismo , Leucócitos Mononucleares/metabolismo , Leucócitos Mononucleares/microbiologia , Porphyromonas gingivalis/metabolismo , Porphyromonas gingivalis/patogenicidade , Apoptose/genética , Proteínas de Bactérias/genética , Humanos , Reação em Cadeia da Polimerase em Tempo Real
5.
An Acad Bras Cienc ; 91(1): e20170831, 2019 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-30916148

RESUMO

Medicinal plants have long been used as an alternative to traditional drugs for the treatment of inflammatory conditions due to the classical side effects and restricted access of various commercially available drugs, such as steroids (GCs) and nonsteroidal anti-inflammatory drugs (NSAIDs). Sambucus australis is a Brazilian herb that is commonly used to treat inflammatory diseases; however, few studies have examined the use of this species in the treatment of inflammatory conditions. The present study aims to evaluate the potential anti-inflammatory activity of S. australis in vitro. We established spleen cell cultures stimulated with pokeweed mitogen (PWM) to evaluate the production of proinflammatory cytokines, such as IL-4, IL-5, IFN-y, and IL-10 (by ELISA), and the expression of the transcription factor NF-kB (by RT-PCR). In addition, we evaluated the levels of nitric oxide in macrophage cultures and the membrane-stabilizing activity of S. australis methanolic extract (EMSA). Treatment with EMSA at concentrations of 100, 50, 25 and 12.5 µg/ml significantly decreased IL-4 (p<0.001) and IL-5 (p<0.001) levels. Treatment with 100 µg/ml EMSA reduced IFN-у (p<0.001) levels. Moreover, at 100 mg/ml, EMSA also increased IL-10 production and reduced NF-kB expression (p<0.01). In macrophage cultures stimulated with LPS, EMSA decreased nitric oxide levels (p<0.001) at all concentrations tested (100, 50, 25 and 12.5 µg/ml). Additionally, EMSA had a protective effect in the erythrocyte membrane stabilization assay. Taken together, these results suggest that S. australis has anti-inflammatory potential in vitro, characterized by the reduction of both inflammatory cytokines and the expression of NF-kB along with the up-regulation of IL-10.


Assuntos
Anti-Inflamatórios/farmacologia , Citocinas/metabolismo , Inflamação/metabolismo , NF-kappa B/metabolismo , Extratos Vegetais/química , Sambucus/química , Animais , Células Cultivadas , Citocinas/análise , Modelos Animais de Doenças , Mediadores da Inflamação/metabolismo , Masculino , Camundongos Endogâmicos BALB C , NF-kappa B/análise , Folhas de Planta/química
6.
Rev. cuba. estomatol ; 56(1): e1796, ene.-mar. 2019. tab
Artigo em Português | LILACS | ID: biblio-1003868

RESUMO

Introdução: A disfunção temporomandibular apresenta etiologia multifatorial, enquadrando-se nesses fatores etiológicos condições psicológicas como a ansiedade. Objetivo: Identificar a prevalência da disfunção temporomandibular, ansiedade e seus respectivos graus nos graduandos. Métodos: Trata-se de um estudo do tipo transversal, observacional, com abordagem indutiva, adotando como estratégia de coleta de dados o Índice Anamnésico de Fonseca e o Inventário de Ansiedade Traço-Estado em uma amostra de 185 estudantes de Odontologia da Universidade Federal de Campina Grande, Patos, Brasil. Os dados foram trabalhados pela estatística descritiva e submetidos ao teste estatístico Qui-quadrado e Exato de Fisher considerado significativo ao nível de 5 porcento. Resultados: Os resultados indicam que a maioria da amostra é composta pelo sexo feminino (67 porcento) com idade média de 21,4 anos. Além disso, 79 porcento dos alunos apresentou algum grau da disfunção, tendo a maior parte (72 porcento) se encaixado no grau leve. A ansiedade estado e a ansiedade traço foram mais prevalentes no grau moderado com 79 porcento e 72 porcento, respectivamente. Não foi identificada associação estatística significativa entre a presença e ausência de Disfunção Temporomandibular e ansiedade, seja ela traço ou estado, na amostra estudada com p= 0,484 e p= 0,297, respectivamente. Conclusão: Conclui-se que houve uma alta prevalência de Disfunção Temporomandibular e ansiedade nos graduandos de Odontologia, destacando-se, assim, a importância da busca detalhada desses sinais e sintomas durante o exame clínico(AU)


Introducción: La disfunción temporomandibular presenta una causa multifactorial, incluyéndose en estos factores causales condiciones psicológicas como la ansiedad. Objetivo: Identificar la prevalencia de la disfunción temporomandibular y la ansiedad y sus respectivos niveles en estudiantes. Métodos: Se trata de un estudio del tipo transversal, observacional y con enfoque inductivo. Se adoptó como estrategia de recolección de datos el índice de anamnesis de Fonseca y el inventario de ansiedad rasgo-estado en una muestra de 185 estudiantes de Odontología de la Universidade Federal de Campina Grande. Los datos fueron trabajados por la estadística descriptiva y sometidos a la prueba estadística de chi cuadrado y exacta de Fisher, considerado significativo al nivel de 5 por ciento. Resultados: Los resultados indican que la mayoría de la muestra eran del sexo femenino (67 por ciento) con una edad promedio de 21,4 años. Además, 79 por ciento de los alumnos presentaron algún nivel de disfunción, y la mayor parte (72 por ciento) se encuadró en el nivel leve. La ansiedad estado y la ansiedad rasgo fueron más prevalentes en el nivel moderado con 79 por ciento y 72 por ciento, respectivamente. No se identificó ninguna asociación estadística significativa entre la presencia y ausencia de disfunción temporomandibular y la ansiedad, sea rasgo o estado, en la muestra investigada, con los respectivos valores de p= 0,484 y p= 0,297. Conclusiones: Hubo una alta prevalencia estadística de disfunción temporomandibular y ansiedad en los estudiantes de Odontología, así que se destaca la importancia de la búsqueda detallada de estos signos y síntomas en el examen clínico(AU)


Introduction: The etiology of temporomandibular dysfunction is multifactorial, and causal factors include psychological conditions such as anxiety. Objective: Identify the prevalence of temporomandibular dysfunction and anxiety as well as their levels among dental students from the Federal University of Campina Grande. Methods: An inductive cross-sectional observational study was conducted of a sample of 185 dental students from the Federal University of Campina Grande. Data collection was based on Fonseca's anamnesis index and the State-Trait Anxiety Inventory. Data were analyzed by descriptive statistics and subjected to the chi-square statistical test and Fisher's exact test, with a significance level of 5 percent. Results: Results show that most of the sample was female (67 percent) with a mean age of 21.4 years. On the other hand, 79 percent of the students had some degree of dysfunction, which was mild in most (72 percent). Anxiety state and anxiety trait were more prevalent on the moderate level with 79 percent and 72 percent respectively. No significant statistical association was identified in the study sample between the presence and absence of temporomandibular dysfunction and anxiety, be it trait or state, with values of p= 0.484 and p= 0.297 respectively. Conclusions: High statistical prevalence was found of temporomandibular dysfunction and anxiety among dental students, hence the importance of a detailed search for those signs and symptoms during clinical examination(AU)


Assuntos
Humanos , Feminino , Adulto , Ansiedade/etiologia , Estudantes de Odontologia/estatística & dados numéricos , Síndrome da Disfunção da Articulação Temporomandibular/epidemiologia , Coleta de Dados/estatística & dados numéricos , Estudos Transversais , Estudo Observacional
7.
Appl Biochem Biotechnol ; 188(3): 798-809, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30706415

RESUMO

The polyphagous caterpillar, Spodoptera frugiperda, has been controlled with either chemical insecticides or transgenic plants such as Bt maize that expresses the cry and/or vip genes of the Bacillus thuringiensis (Bt) bacterium. Despite the efficiency of Bt toxins in lepidopteran control, populations resistant to Bt plants have emerged in different locations around the world. Thus, understanding how combined proteins interact against pests can assist resistance control and management. This work demonstrated the toxicity of Cry1Ab, Cry1Ac, Cry1Ca, Cry1Ea, Cry2Aa, Cry2Ab, Vip3Aa, and Vip3Ca in single and combined assays against S. frugiperda neonatal larvae. All protein mixtures had synergistic action in the control of the larvae. The Vip3Aa + Cry1Ab mixture had the highest toxicity, sequentially followed by Vip3Aa + Cry2Ab, Cry1Ab + Cry2Ab + Vip3Aa, Cry1Ea + Cry1Ca, Cry1Ab + Cry2Ab, Vip3Ca + Cry1Ea, and Vip3Ca + Cry1Ca. Cry1Ab, Cry1Ac, Cry2Ab, and Vip3Aa bound to more than one site on the brush border membrane vesicles (BBMV) of S. frugiperda. The Cry1Ab and Cry1Ac proteins share binding site, while Cry1Ab does not share binding site with the Cry2Aa and Cry2Ab proteins. The Vip3Aa protein does not share receptors with the tested Cry1 and Cry2. The results suggest that combination these tested proteins may increase toxicity against S. frugiperda neonates.


Assuntos
Bacillus thuringiensis/metabolismo , Proteínas de Bactérias/toxicidade , Larva/efeitos dos fármacos , Controle Biológico de Vetores/métodos , Spodoptera/efeitos dos fármacos , Animais , Proteínas de Bactérias/metabolismo , Western Blotting , Ligantes , Spodoptera/crescimento & desenvolvimento
8.
Nat Commun ; 10(1): 880, 2019 02 20.
Artigo em Inglês | MEDLINE | ID: mdl-30787307

RESUMO

Asthma is a complex disease with striking disparities across racial and ethnic groups. Despite its relatively high burden, representation of individuals of African ancestry in asthma genome-wide association studies (GWAS) has been inadequate, and true associations in these underrepresented minority groups have been inconclusive. We report the results of a genome-wide meta-analysis from the Consortium on Asthma among African Ancestry Populations (CAAPA; 7009 asthma cases, 7645 controls). We find strong evidence for association at four previously reported asthma loci whose discovery was driven largely by non-African populations, including the chromosome 17q12-q21 locus and the chr12q13 region, a novel (and not previously replicated) asthma locus recently identified by the Trans-National Asthma Genetic Consortium (TAGC). An additional seven loci reported by TAGC show marginal evidence for association in CAAPA. We also identify two novel loci (8p23 and 8q24) that may be specific to asthma risk in African ancestry populations.


Assuntos
Afro-Americanos/genética , Asma/genética , Predisposição Genética para Doença/genética , Asma/epidemiologia , Cromossomos Humanos Par 12/genética , Cromossomos Humanos Par 17/genética , Cromossomos Humanos Par 8/genética , Loci Gênicos , Estudo de Associação Genômica Ampla , Hispano-Americanos/genética , Humanos , Polimorfismo de Nucleotídeo Único/genética , Estados Unidos/epidemiologia
9.
Pediatr Pulmonol ; 54(2): 125-132, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30548437

RESUMO

BACKGROUND: Genetic variants underlying African ancestry have been suggested be implicated in the ethnic-racial inequalities reported for asthma and allergies. OBJECTIVES: To investigate the association between individual African ancestry and asthma symptoms, atopic and non-atopic asthma, and atopy in children. METHODS: A cross-sectional study encompassing 1190 individuals was conducted. African biogeographic ancestry was estimated using 370 539 genome-wide SNPs. Serum levels of specific IgE were measured, and skin prick test (SPT) performed for the most common local aeroallergens. Information on asthma symptoms was obtained by applying the International Study of Allergy and Asthma in Childhood questionnaire. The associations between the proportion of individual African ancestry and the outcomes investigated were analyzed through multivariate models adjusted for socio-environmental variables, infections markers, and psychosocial factors. RESULTS: Each 20% increase in the proportion of African ancestry was negatively associated with SPT reactivity (OR: 0.79, 95%CI: 0.66-0.96) and positively associated with asthma symptoms in non-atopic individuals (OR: 1.40, 95%CI: 1.03-1.89). We estimated that socioeconomic status and number of infections mediated 28.4% of the effect of African ancestry on SPT reactivity, while 20.2% of the effect on non-atopic asthma was explained by socioeconomic status and behavioral problems in children. CONCLUSIONS: The negative association observed between African ancestry and atopy is most probably explained by unobserved environmental or social factors that covariate with ancestry. For non-atopic asthma, in turn, putative genetic variants of risk underlying African ancestry may play some role.

10.
Cytokine ; 113: 177-184, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30539779

RESUMO

Asthma and allergy affect hundreds of millions of people from childhood to old age. In most of them, the inflammatory process of respiratory allergies involves the participation of type 2 cytokines, derived from T helper-2 (Th2)-cell, and Group 2 Innate Lymphoid (ILC2) Cells. An efficient memory Th2 cell response is dependent on IL-13 produced by ILC2s, causing allergic lung inflammation and elevated serum levels of immunoglobulin E. ILC2 cells are derived from common lymphoid progenitors and their growing depends on the transcription factor RORA. The aim of this work was to identify genetic variants in RORA associated with asthma phenotypes and allergy markers. Genomic DNA samples of 1246 individuals participating from Social Changes Asthma and Allergy in Latin America Program (SCAALA) have been genotyped using Illumina Human 2.5 Omni Beadchip. Logistics regressions have been performed to analyze the association among RORA variants and asthma, skin prick tests (SPT), specific IgE and type 2 cytokine production. Twelve single nucleotide variants (SNVs) were significantly associated with atopy (P < 0.01), in which four of them, rs10162630, rs17191519, rs17270243, and rs55796775 and their haplotypes were strongly and positively associated (P < 0.001). Furthermore, these variants increased the RORA gene expression in silico analysis. Other SNVs in RORA were associated with allergy markers, atopic and non-atopic asthma. Therefore, it is believed that variants in RORA gene may influence immunologic features of asthma and allergies and could be possible targets for future treatment of allergic diseases.

11.
Rev Col Bras Cir ; 45(6): e1978, 2018 Nov 29.
Artigo em Português, Inglês | MEDLINE | ID: mdl-30517359

RESUMO

OBJECTIVE: to study the epidemiological data of patients suffering from buccomaxillofacial trauma treated at a referral hospital in the State of Paraíba. METHODS: we conducted a cross-sectional study of inductive approach, with a comparative statistical procedure and research technique by field direct documentation. The sample comprised hospital records obtained from January 2016 to December 2017 of patients attended by the Service of Buccomaxillofacial Surgery and Traumatology of this hospital, and consisted of 332 patients according to the study's eligibility criteria. Two previously calibrated examiners collected and analyzed the data, both descriptively and inferentially. RESULTS: males sustained the majority of facial trauma (83.1%), mainly in the third decade of life (32.2%). Motorcycle accidents were the most common etiology of trauma for both genders. In relation to inferential statistics with a margin of error of 5%, there was no significant association (p>0.05) between the genders and the trauma etiological factors. The bones of the nose (38.2%) were the most affected bones and the most frequent soft tissue injury was edema, in 50.9% of cases. Only 20.8% of the patients with bone fractures were polytraumatized. CONCLUSION: the victims of oral and maxillofacial trauma attended at our hospital are predominantly men in the third decade of life, involved in motorcycle accidents and sustaining lesions in the nose bones nose.

12.
Histol Histopathol ; : 18038, 2018 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-30207375

RESUMO

A crucial component of the integration between foreign implants and the host is angiogenesis. However, to date, none of the available techniques and/or endothelial markers employed to assess angiogenesis in the implant/host interface seems to be able to highlight vascular structures convincingly. In the present study we investigated and compared the expression of two endothelial cell markers: platelet endothelial cell adhesion molecule (PECAM-1) (CD31) and endoglin (CD105) using immunohistochemistry (IHC) and immunofluorescence (IF) to identify and quantify newly formed blood vessels in subcutaneous implants of polyether-polyurethane sponge of formalin-fixed paraffin-embedded tissue. At day 14 post implantation the discs of the synthetic matrix were removed and processed for histological and morphometric analysis. In IHC staining for CD31 antibody the number of vessels was 2.27±0.69 and 5.25±0.46 for CD105. In IF for CD31 the number of vessels was 15.36±1.295 and 10.54±0.8213 for CD105. The level of cross-reaction was lesser in IF images compared with IHC images. Co-localization of CD31/CD105 using confocal images showed positive correlation (Pearson's co-relation and Manders' equation). The double labeling for blood vessels using the IF technique for CD31/CD105 may be an important tool for evaluation of angiogenesis in biomaterial/host integration.

13.
Parasite Immunol ; : e12588, 2018 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-30188574

RESUMO

Brazil is a middle-income country undergoing the epidemiological transition. Effects of changes in daily life habits and access to clean water, sanitation and urban services on a growing urban population have contributed to a double burden of both infectious and noncommunicable chronic diseases. Studies have indicated that parasite infections may modulate the human immune system and influence the development of allergic conditions such as asthma. However, there is no consensus in the published literature on the effects of parasitic infections on allergy, perhaps as a consequence of factors determining the epidemiology of these infections that vary between populations such as age of first infection, duration and chronicity of infections, parasite burden and species, and host genetic susceptibility. In this review, we discuss the observations from Brazil concerning the relationship between parasite infections and allergy.

14.
Sci Rep ; 8(1): 14475, 2018 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-30262839

RESUMO

The genetic architecture of asthma was relatively well explored. However, some work remains in the field to improve our understanding on asthma genetics, especially in non-Caucasian populations and with regards to commonly neglected genetic variants, such as Copy Number Variations (CNVs). In the present study, we investigated the contribution of CNVs on asthma risk among Latin Americans. CNVs were inferred from SNP genotyping data. Genome wide burden and association analyses were conducted to evaluate the impact of CNVs on asthma outcome. We found no significant difference in the numbers of CNVs between asthmatics and non-asthmatics. Nevertheless, we found that CNVs are larger in patients then in healthy controls and that CNVs from cases intersect significantly more genes and regulatory elements. We also found that a deletion at 6p22.1 is associated with asthma symptoms in children from Salvador (Brazil) and in young adults from Pelotas (Brazil). To support our results, we conducted an in silico functional analysis and found that this deletion spans several regulatory elements, including two promoter elements active in lung cells. In conclusion, we found robust evidence that CNVs could contribute for asthma susceptibility. These results uncover a new perspective on the influence of genetic factors modulating asthma risk.

15.
RGO (Porto Alegre) ; 66(3): 212-218, July-Sept. 2018. tab
Artigo em Inglês | LILACS-Express | ID: biblio-984905

RESUMO

ABSTRACT Objective: To determine the profile of children from 6 to 12 years of age, in relation to anxiety towards dental treatment and evaluate the main factors that trigger anxiety in this population. Methods: The sample was composed of 200 children with an average age of 8.5 years who attended the Clinic of the School of Dentistry of the Federal University of Campina Grande and the Basic Healthcare Units of the municipality of Patos, Paraíba, Brazil between June 2015 and May 2016. Anxiety was assessed using the Dental Anxiety Scale and the Venham Picture Test. The Chi-Square test and Fisher's exact test were used to explore the level of significance of the associations between the variables Results: Anxiety was observed in the majority of children (Dental Anxiety Scale - 89% and Venham Picture Test - 70.5%) and the predominant levels were low to moderate. The child´s age group was significantly associated with anxiety (p=0.014) by the Venham Picture Test, while gender did not present this correlation. Anxiety was influenced by all the factors studied: making the appointment, waiting room, rotary instrument and periodontal scaling. Conclusion: The majority of children presented anxiety and the trigger factors: making the appointment, waiting room, rotary instrument and periodontal scaling contributed considerably to its development.


RESUMO Objetivo: Determinar o perfil das crianças de 6 a 12 anos de idade, com relação à ansiedade frente ao tratamento odontológico e avaliar os principais fatores desencadeadores de ansiedade nesta população. Métodos: A amostra foi composta por 200 crianças com idade média de 8,5 anos que frequentaram a Clínica-Escola de Odontologia da Universidade Federal de Campina Grande e as Unidades Básicas de Saúde da Família do município de Patos-PB entre junho de 2015 e maio de 2016. A ansiedade foi avaliada utilizando a Dental Anxiety Scale e o Venham Picture Test. O teste Qui-Quadrado e Exato de Fisher foram usados para explorar o nível de significância das associações entre as variáveis. Resultados: A ansiedade foi observada na maioria das crianças (Dental Anxiety Scale - 89% e Venham Picture Test - 70,5%) e os níveis predominantes foram baixo e moderado. A faixa etária da criança foi associada significativamente à ansiedade (p=0,014) através do teste Venham Picture Test, ao passo que o gênero não apresentou essa correlação. A ansiedade sofreu influência de todos os fatores estudados: marcação da consulta, sala de espera, caneta odontológica e raspagem periodontal. Conclusão: A maioria das crianças apresentou ansiedade e os fatores desencadeadores marcação da consulta, sala de espera, caneta odontológica e raspagem periodontal atuaram consideravelmente para o seu desenvolvimento.

16.
Nutrients ; 10(8)2018 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-30126176

RESUMO

No studies showing that food consumption is a modifier of the association of variants of the leptin receptor gene (LEPR) with body weight have involved a Brazilian population. The aim of this study was to evaluate the modifying effect of dietary intake on the association between the LEPR gene and excess weight. In this study, 1211 children and adolescents aged 4⁻11 years were assessed. Participants were genotyped for 112 single-nucleotide variants of the LEPR gene. Anthropometric measurements were performed, and dietary data were obtained. Logistic regressions were used to study the associations of interest. Of the participants, 13.4% were overweight/obese. The risk allele (G) of the rs1137100 variant was associated with excess weight in individuals with fat consumption below the median (odds ratio OR = 1.92; 95% confidence interval CI = 1.18⁻3.14), with daily frequency of consumption of drink/artificial juice (OR = 2.15; 95% CI = 1.26⁻3.68) and refined cereals (OR = 2.17; 95% CI = 1.31⁻3.62) above the median. The risk allele (G) of variant rs1177681 was also associated with excess weight (OR = 2.74; 95% CI = 1.65⁻4.57) in subjects with a daily frequency of refined cereal consumption above the median. The association between LEPR and excess weight can be modulated by the type and distribution of dietary fatty acids, sugary drinks, and refined cereals.

17.
ERJ Open Res ; 4(3)2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30151371

RESUMO

The World Asthma Phenotypes (WASP) study started in 2016 and has been conducted in five centres, in the UK, New Zealand, Brazil, Ecuador and Uganda. The objectives of this study are to combine detailed biomarker and clinical information in order to 1) better understand and characterise asthma phenotypes in high-income countries (HICs) and low and middle-income countries (LMICs), and in high and low prevalence centres; 2) compare phenotype characteristics, including clinical severity; 3) assess the risk factors for each phenotype; and 4) assess how the distribution of phenotypes differs between high prevalence and low prevalence centres. Here we present the rationale and protocol for the WASP study to enable other centres around the world to carry out similar analyses using a standardised protocol. Large collaborative and integrative studies like this are essential to further our understanding of asthma phenotypes. The findings of this study will help elucidate the aetiological mechanisms of asthma and might potentially identify new causes and guide the development of new treatments, thereby enabling better management and prevention of asthma in both HICs and LMICs.

18.
Mol Immunol ; 101: 294-302, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30032071

RESUMO

Atopic asthma, which is characterized by the chronic inflammation and morbidity of airways, is a disease of great complexity, and multiple genetic and environmental factors are involved in its etiology. In the first genome-wide association study (GWAS) conducted in Brazil for asthma, a positive association was found between atopic asthma and a variant (rs1999071), which is located between the DAD1 and OXA1L genes, although neither gene has previously been reported to be associated with asthma or allergies. The DAD1 gene is involved in the regulation of programmed cell death, and OXA1L is involved in biogenesis and mitochondrial oxidative phosphorylation. This study aimed to evaluate how polymorphisms in DAD1 and OXA1L are associated with asthma and markers of atopy in individuals from the Salvador cohort of the SCAALA (Social Change Asthma and Allergy in Latin America) program. The DNA of 1220 individuals was genotyped using the Illumina 2.5 Human Omni Bead chip. Logistic regression analyses were performed with PLINK 1.9 software to verify the association between DAD1 and OXA1L polymorphisms and asthma and atopic markers, adjusted for sex, age, helminth infections and ancestry markers, using an additive model. The DAD1 and OXA1L genes were associated with some of the evaluated phenotypes, such as asthma, skin prick test (SPT), specific IgE for aeroallergens, and Th1/Th2-type cytokine production. Using qPCR, as well as in silico gene expression analysis, we have demonstrated that some of the polymorphisms in both genes are able to affect their respective gene expression levels. In addition, DAD1 was over-expressed in asthmatic patients when compared with controls. Thus, our findings demonstrate that variants in both the DAD1 and OXA1L genes may affect atopy and asthma in a Latin American population with a high prevalence of asthma.

19.
SSM Popul Health ; 4: 301-306, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29854914

RESUMO

Racial inequalities are observed for different diseases and are mainly caused by differences in socioeconomic status between ethnoracial groups. Genetic factors have also been implicated, and recently, several studies have investigated the association between biogeographical ancestry (BGA) and complex diseases. However, the role of BGA as a proxy for non-genetic health determinants has been little investigated. Similarly, studies comparing the association of BGA and self-reported skin colour with these determinants are scarce. Here, we report the association of BGA and self-reported skin colour with socioenvironmental conditions and infections. We studied 1246 children living in a Brazilian urban poor area. The BGA was estimated using 370,539 genome-wide autosomal markers. Standardised questionnaires were administered to the children's guardians to evaluate socioenvironmental conditions. Infection (or pathogen exposure) was defined by the presence of positive serologic test results for IgG to seven pathogens (Toxocara spp, Toxoplasma gondii, Helicobacter pylori, and hepatitis A, herpes simplex, herpes zoster and Epstein-Barr viruses) and the presence of intestinal helminth eggs in stool samples (Ascaris lumbricoides and Trichiuris trichiura). African ancestry was negatively associated with maternal education and household income and positively associated with infections and variables, indicating poorer housing and living conditions. The self-reported skin colour was associated with infections only. In stratified analyses, the proportion of African ancestry was associated with most of the outcomes investigated, particularly among admixed individuals. In conclusion, BGA was associated with socioenvironmental conditions and infections even in a low-income and highly admixed population, capturing differences that self-reported skin colour miss. Importantly, our findings suggest caution in interpreting significant associations between BGA and diseases as indicative of the genetic factors involved.

20.
Sci Rep ; 8(1): 9805, 2018 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-29955082

RESUMO

The global situation of diseases transmitted by arthropod-borne viruses such as Dengue (DENV), Yellow Fever (YFV), Chikungunya (CHIKV) and Zika (ZIKV) viruses is alarming and treatment of human infection by these arboviruses faces several challenges. The discovery of broad-spectrum antiviral molecules, able to inactivate different groups of viruses, is an interesting approach. The viral envelope is a common structure among arboviruses, being a potential target for antivirals. Porphyrins are amphipathic molecules able to interact with membranes and absorb light, being widely used in photodynamic therapy. Previously, we showed that heme, Co-protoporphyrin IX (CoPPIX) and Sn-protoporphyrin IX (SnPPIX) directly inactivate DENV and YFV infectious particles. Here we demonstrate that the antiviral activity of these porphyrins can be broadened to CHIKV, ZIKV, Mayaro virus, Sindbis virus and Vesicular Stomatitis virus. Porphyrin treatment causes viral envelope protein loss, affecting viral morphology, adsorption and entry into target cells. Also, light-stimulation enhanced the SnPPIX activity against all tested arboviruses. In summary, CoPPIX and SnPPIX were shown to be efficient broad-spectrum compounds to inactivate medically and veterinary important viruses.

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