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1.
Front Cell Infect Microbiol ; 12: 1057774, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36439218

RESUMO

The endoplasmic reticulum (ER) of higher eukaryotic cells forms an intricate membranous network that serves as the main processing facility for folding and assembling of secreted and membrane proteins. The ER is a highly dynamic organelle that interacts with other intracellular structures, as well as endosymbiotic pathogenic and non-pathogenic microorganisms. A strict ER quality control (ERQC) must work to ensure that proteins entering the ER are folded and processed correctly. Unfolded or misfolded proteins are usually identified, selected, and addressed to Endoplasmic Reticulum-Associated Degradation (ERAD) complex. Conversely, when there is a large demand for secreted proteins or ER imbalance, the accumulation of unfolded or misfolded proteins activates the Unfold Protein Response (UPR) to restore the ER homeostasis or, in the case of persistent ER stress, induces the cell death. Pathogenic trypanosomatids, such as Trypanosoma cruzi, Trypanosoma brucei and Leishmania spp are the etiological agents of important neglected diseases. These protozoans have a complex life cycle alternating between vertebrate and invertebrate hosts. The ER of trypanosomatids, like those found in higher eukaryotes, is also specialized for secretion, and depends on the ERAD and non-canonical UPR to deal with the ER stress. Here, we reviewed the basic aspects of ER biology, organization, and quality control in trypanosomatids. We also focused on the unusual way by which T. cruzi, T. brucei, and Leishmania spp. respond to ER stress, emphasizing how these parasites' ER-unrevealed roads might be an attractive target for chemotherapy.


Assuntos
Leishmania , Resposta a Proteínas não Dobradas , Degradação Associada com o Retículo Endoplasmático , Retículo Endoplasmático/metabolismo , Estresse do Retículo Endoplasmático , Proteínas/metabolismo , Leishmania/metabolismo
2.
Front Mol Biosci ; 9: 971811, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36275617

RESUMO

Gene expression in pathogenic protozoans of the family Trypanosomatidae has several novel features, including multiple eIF4F-like complexes involved in protein synthesis. The eukaryotic eIF4F complex, formed mainly by eIF4E and eIF4G subunits, is responsible for the canonical selection of mRNAs required for the initiation of mRNA translation. The best-known complexes implicated in translation in trypanosomatids are based on two related pairs of eIF4E and eIF4G subunits (EIF4E3/EIF4G4 and EIF4E4/EIF4G3), whose functional distinctions remain to be fully described. Here, to define interactomes associated with both complexes in Trypanosoma brucei procyclic forms, we performed parallel immunoprecipitation experiments followed by identification of proteins co-precipitated with the four tagged eIF4E and eIF4G subunits. A number of different protein partners, including RNA binding proteins and helicases, specifically co-precipitate with each complex. Highlights with the EIF4E4/EIF4G3 pair include RBP23, PABP1, EIF4AI and the CRK1 kinase. Co-precipitated partners with the EIF4E3/EIF4G4 pair are more diverse and include DRBD2, PABP2 and different zinc-finger proteins and RNA helicases. EIF4E3/EIF4G4 are essential for viability and to better define their role, we further investigated their phenotypes after knockdown. Depletion of either EIF4E3/EIF4G4 mRNAs lead to aberrant morphology with a more direct impact on events associated with cytokinesis. We also sought to identify those mRNAs differentially associated with each complex through CLIP-seq with the two eIF4E subunits. Predominant among EIF4E4-bound transcripts are those encoding ribosomal proteins, absent from those found with EIF4E3, which are generally more diverse. RNAi mediated depletion of EIF4E4, which does not affect proliferation, does not lead to changes in mRNAs or proteins associated with EIF4E3, confirming a lack of redundancy and distinct roles for the two complexes.

3.
J Fungi (Basel) ; 8(6)2022 May 25.
Artigo em Inglês | MEDLINE | ID: mdl-35736039

RESUMO

Candida albicans is the main cause of superficial candidiasis. While the antifungals available are defied by biofilm formation and resistance emergence, antimicrobial photodynamic inactivation (aPDI) arises as an alternative antifungal therapy. The tetracationic metalloporphyrin Zn(II) meso-tetrakis(N-n-hexylpyridinium-2-yl)porphyrin (ZnTnHex-2-PyP4+) has high photoefficiency and improved cellular interactions. We investigated the ZnTnHex-2-PyP4+ as a photosensitizer (PS) to photoinactivate yeasts and biofilms of C. albicans strains (ATCC 10231 and ATCC 90028) using a blue light-emitting diode. The photoinactivation of yeasts was evaluated by quantifying the colony forming units. The aPDI of ATCC 90028 biofilms was assessed by the MTT assay, propidium iodide (PI) labeling, and scanning electron microscopy. Mammalian cytotoxicity was investigated in Vero cells using MTT assay. The aPDI (4.3 J/cm2) promoted eradication of yeasts at 0.8 and 1.5 µM of PS for ATCC 10231 and ATCC 90028, respectively. At 0.8 µM and same light dose, aPDI-treated biofilms showed intense PI labeling, about 89% decrease in the cell viability, and structural alterations with reduced hyphae. No considerable toxicity was observed in mammalian cells. Our results introduce the ZnTnHex-2-PyP4+ as a promising PS to photoinactivate both yeasts and biofilms of C. albicans, stimulating studies with other Candida species and resistant isolates.

4.
J Appl Microbiol ; 132(6): 4300-4309, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35338561

RESUMO

AIMS: To synthesize and evaluate the antifungal activity of poly(methacrylic acid)-silver nanoparticles (PMAA-AgNPs) against nine Candida albicans isolated from clinical specimens. METHODS AND RESULTS: The effects of PMAA-AgNPs-fluconazole combination was analysed by checkerboard methodology. The synergistic potential of PMAA-AgNPs-fluconazole was determined by the fractional inhibitory concentration index (FICI). The inhibition of germ tube formation and the determination of PMAA-AgNPs cytotoxicity were also performed. All C. albicans strains were susceptible to PMAA-AgNPs and resistant to fluconazole. PMAA-AgNPs at subinhibitory concentrations restored the susceptibility of resistant C. albicans to fluconazole, whose FICI ranged from 0.3 to 0.5. The synergistic interaction of the combination was observed in eight of nine strains. The PMAA-AgNPs-fluconazole combination was also able to inhibit the germ tube formation. PMAA-AgNPs showed a dose-dependent decrease in viability for cells tested, with 50% cytotoxic concentration (CC50 ) values of 6.5, 4.9 and 6.8 µg ml-1 for macrophages, fibroblasts and Vero cells, respectively. CONCLUSIONS: This study demonstrated that, in general, PMAA-AgNPs acts synergistically in combination with fluconazole, inhibiting fluconazole-resistant C. albicans strains. PMAA-AgNPs-fluconazole combination was also able to inhibit germ tube formation, an important virulence factor. Inhibitory effect of PMAA-AgNPs alone or in combination was higher in C. albicans than in mammalian cells. SIGNIFICANCE AND IMPACT OF STUDY: This study shows the potential of PMAA-AgNPs combined with fluconazole to inhibit fluconazole-resistant C. albicans strains.


Assuntos
Fluconazol , Nanopartículas Metálicas , Animais , Antifúngicos/farmacologia , Candida albicans , Chlorocebus aethiops , Farmacorresistência Fúngica , Sinergismo Farmacológico , Fluconazol/farmacologia , Mamíferos , Metacrilatos , Testes de Sensibilidade Microbiana , Prata/farmacologia , Células Vero
5.
J Biomol Struct Dyn ; 40(12): 5462-5480, 2022 08.
Artigo em Inglês | MEDLINE | ID: mdl-33459182

RESUMO

In this work we aimed to perform an in silico predictive screening, docking and molecular dynamic study to identify 1,2,3-triazole-phthalimide derivatives as drug candidates against SARS-CoV-2. The in silico prediction of pharmacokinetic and toxicological properties of hundred one 1,2,3-triazole-phtalimide derivatives, obtained from SciFinder® library, were investigated. Compounds that did not show good gastrointestinal absorption, violated the Lipinski's rules, proved to be positive for the AMES test, and showed to be hepatotoxic or immunotoxic in our ADMET analysis, were filtered out of our study. The hit compounds were further subjected to molecular docking on SARS-CoV-2 target proteins. The ADMET analysis revealed that 43 derivatives violated the Lipinski's rules and 51 other compounds showed to be positive for the toxicity test. Seven 1,2,3-triazole-phthalimide derivatives (A7, A8, B05, E35, E38, E39, and E40) were selected for molecular docking and MFCC-ab initio analysis. The results of molecular docking pointed the derivative E40 as a promising compound interacting with multiple target proteins of SARS-CoV-2. The complex E40-Mpro was found to have minimum binding energy of -10.26 kcal/mol and a general energy balance, calculated by the quantum mechanical analysis, of -8.63 eV. MD simulation and MMGBSA calculations confirmed that the derivatives E38 and E40 have high binding energies of -63.47 ± 3 and -63.31 ± 7 kcal/mol against SARS-CoV-2 main protease. In addition, the derivative E40 exhibited excellent interaction values and inhibitory potential against SAR-Cov-2 main protease and viral nucleocapsid proteins, suggesting this derivative as a potent antiviral for the treatment and/or prophylaxis of COVID-19.Communicated by Ramaswamy H. Sarma.


Assuntos
Humanos , Simulação de Acoplamento Molecular , Simulação de Dinâmica Molecular , Ftalimidas/farmacologia , Inibidores de Proteases/química , SARS-CoV-2 , Triazóis/farmacologia
6.
São Paulo; Instituto de Saúde; 2022. 122 p.
Não convencional em Português | Sec. Est. Saúde SP, CONASS, SESSP-ISPROD, Sec. Est. Saúde SP | ID: biblio-1399213

RESUMO

Em janeiro de 2020 a Organização Mundial de Saúde declarou Emergência de Saúde Pública de Importância Internacional devido à doença denominada COVID-19, causada pelo coronavírus SARS-CoV-2. Em 11 de março foi declarada a pandemia da COVID-19 que apresenta desafios sem precedentes para políticas e sistemas de saúde de todo o mundo. O Brasil já registrou mais de meio milhão de mortes desde o início da pandemia, segundo boletim do Conselho Nacional de Secretários de Saúde (Conass) do dia 21 de junho de 2021


Assuntos
Atenção Primária à Saúde , Saúde Pública , Comunicação em Saúde , Avaliação da Tecnologia Biomédica , Saúde Mental
7.
Biomolecules ; 11(12)2021 11 24.
Artigo em Inglês | MEDLINE | ID: mdl-34944393

RESUMO

Titanium and its alloys are used as biomaterials for medical and dental applications, due to their mechanical and physical properties. Surface modifications of titanium with bioactive molecules can increase the osseointegration by improving the interface between the bone and implant. In this work, titanium dioxide nanotubes (TiO2NTs) were functionalized with a lectin from the plasma of the fish Oreochromis niloticus aiming to favor the adhesion and proliferation of osteoblast-like cells, improving its biocompatibility. The TiO2NTs were obtained by anodization of titanium and annealed at 400 °C for 3 h. The resulting TiO2NTs were characterized by high-resolution scanning electron microscopy. The successful incorporation of OniL on the surface of TiO2NTs, by spin coating, was demonstrated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIE), and attenuated total reflection-Fourier transform infrared spectrum (ATR-FTIR). Our results showed that TiO2NTs were successfully synthesized in a regular and well-distributed way. The modification of TiO2NTs with OniL favored adhesion, proliferation, and the osteogenic activity of osteoblast-like cells, suggesting its use to improve the quality and biocompatibility of titanium-based biomaterials.


Assuntos
Ciclídeos/metabolismo , Lectinas/química , Osteoblastos/citologia , Titânio/farmacologia , Ligas , Animais , Adesão Celular/efeitos dos fármacos , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Espectroscopia Dielétrica , Microscopia Eletrônica de Varredura , Nanotubos , Osteogênese , Propriedades de Superfície , Titânio/química
8.
Biochim Biophys Acta Gen Subj ; 1865(7): 129897, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-33811942

RESUMO

BACKGROUND: Photodynamic inactivation (PDI) is emerging as a promising alternative for cutaneous leishmaniasis (CL). The chemotherapy currently used presents adverse effects and cases of drug resistance have been reported. ZnTnHex-2-PyP4+ is a porphyrin with a high potential as a photosensitizer (PS) for PDI, due to its photophysical properties, structural stability, and cationic/amphiphilic character that can enhance interaction with cells. This study aimed to investigate the photodynamic effects mediated by ZnTnHex-2-PyP4+ on Leishmania parasites. METHODS: ZnTnHex-2-PyP4+ stability was evaluated using accelerated solvolysis conditions. The photodynamic action on promastigotes was assessed by (i) viability assays, (ii) mitochondrial membrane potential evaluation, and (iii) morphological analysis. The PS-promastigote interaction was studied. PDI on amastigotes and the cytotoxicity on macrophages were also analyzed. RESULTS: ZnTnHex-2-PyP4+, under submicromolar concentration, led to immediate inactivation of more than 95% of promastigotes. PDI promoted intense mitochondrial depolarization, loss of the fusiform shape, and plasma membrane wrinkling in promastigotes. Fluorescence microscopy revealed a punctate PS labeling in the parasite cytoplasm. PDI also led to reductions of ca. 64% in the number of amastigotes/macrophage and 70% in the infection index after a single treatment session. No noteworthy toxicity was observed on mammalian cells. CONCLUSIONS: ZnTnHex-2-PyP4+ is stable against demetallation and more efficient as PS than the ethyl analogue ZnTE-2-PyP4+, indicating readiness for evaluation in in vivo studies as an alternative approach to CL. GENERAL SIGNIFICANCE: This report highlighted promising photodynamic effects mediated by ZnTnHex-2-PyP4+ on Leishmania parasites, opening up perspectives for applications in CL pre-clinical assays and PDI of other microorganisms.


Assuntos
Leishmania/efeitos dos fármacos , Macrófagos/efeitos dos fármacos , Metaloporfirinas/farmacologia , Fotoquimioterapia/métodos , Zinco/química , Animais , Feminino , Leishmania/crescimento & desenvolvimento , Metaloporfirinas/química , Camundongos , Camundongos Endogâmicos BALB C
9.
Rev Soc Bras Med Trop ; 54: e0055-2020, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33759914

RESUMO

INTRODUCTION: Mayaro virus (MAYV) was found in Pará state, Brazil, in 1955. Since then, sporadic outbreaks have occurred in different regions of the country. METHODS: Serum sample were collected from 49 individuals in 2016 and were initially tested for dengue virus (DENV) by real-time (RT) polymerase chain reaction (PCR). DENV-negative samples were tested for MAYV and Oropouche virus (OROV) by multiplexed RT quantitative PCR. RESULTS: All samples were negative for DENV and OROV, but MAYV was detected in four samples. CONCLUSIONS: Differential diagnoses of acute febrile syndrome are required, especially in regions where several arboviruses with similar clinical manifestations are endemic.


Assuntos
Alphavirus , Arbovírus , Dengue , Alphavirus/genética , Arbovírus/genética , Brasil/epidemiologia , Dengue/diagnóstico , Dengue/epidemiologia , Surtos de Doenças , Humanos , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa
10.
Plant Physiol Biochem ; 168: 116-127, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34628173

RESUMO

Salinity is a major issue affecting photosynthesis and crop production worldwide. High salinity induces both osmotic and ionic stress in plant tissues as a result of complex interactions among morphological, physiological, and biochemical processes. Salinity, in turn, can provoke inactivation of some enzymes in the Calvin-Benson cycle and therefore affect the fine adjustment of electron transport in photosystem I and carbon related reactions. Here, we used three contrasting Jatropha curcas genotypes namely CNPAE183 (considered tolerant to salinity), CNPAE218 (sensible), and JCAL171 (intermediate) to understand salinity responses. By performing a long-term (12 months) experiment in land conditions, we investigated distinct mechanisms used by J. curcas to cope with threatening salinity effects by analyzing gas exchange, mineral nutrition and metabolic responses. First, our results highlighted the plasticity of stomatal development and density in J. curcas under salt stress. It also demonstrated that the CNPAE183 presented higher salt-tolerance whereas CNPAE218 displayed a more sensitive salt-tolerance response. Our results also revealed that both tolerance and sensitivity to salinity were connected with an extensive metabolite reprogramming in the Calvin-Benson cycle and Tricarboxylic Acid cycle intermediates with significant changes in amino acids and organic acids. Collectively, these results indicate that the CNPAE183 and CNPAE218 genotypes demonstrated certain characteristics of salt-tolerant-like and salt-sensitive-like genotypes, respectively. Overall, our results highlight the significance of metabolites associated with salt responses and further provide a useful selection criterion in during screening for salt tolerance in J. curcas in breeding programmes.


Assuntos
Jatropha , Jatropha/genética , Fotossíntese , Salinidade , Tolerância ao Sal , Estresse Fisiológico
11.
J Biomol Struct Dyn ; : 1-16, 2021 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-34632943

RESUMO

Cutaneous Leishmaniasis (CL) is a neglected disease characterized by highest morbidity rates worldwide. The available treatment for CL has several limitations including serious side effects and resistance to the treatment. Herein we aimed to evaluate the activity of essential oil from the peel of Myrciaria floribunda fruits (MfEO) on Leishmania amazonensis. The cytotoxic potential of MfEO on host mammalian cells was evaluated by MTT. The in vitro leishmanicidal effects of MfEO were investigated on the promastigote and intracellular amastigote forms. The ultrastructural changes induced by MfEO were evaluated by Scanning Electron Microscopy (SEM). The molecular docking of the major compounds δ-Cadinene, γ-Cadinene, γ-Muurolene, α-Selinene, α-Muurolene and (E)-Caryophyllene onto the enzymes trypanothione reductase (TreR) and sterol 14-alpha demethylase (C14DM) were performed. Our results showed that MfEO presented moderate cytotoxicity for Vero cells and macrophages. The MfEO inhibited the growth of promastigote and the survival of intracellular amastigotes, in a dose- and time- dependent way. The MfEO presented high selectivity towards amastigote forms, being 44.1 times more toxic for this form than to macrophages. Molecular docking analysis showed that the major compounds of MfEO interact with Leishmania enzymes and that δ-Cadinene (δ-CAD) presented favorable affinity energy values over TreR and C14DM enzymes, when compared with the other major constituents. Molecular dynamics (MD) simulation studies revealed a stable binding of δ-CAD with lowest binding free energy values in MMGBSA assay. Our results suggested that δ-CAD may be a potent inhibitor of TreR and C14DM enzymes. Communicated by Ramaswamy H. Sarma.

12.
Cien Saude Colet ; 26(suppl 2): 3671-3682, 2021.
Artigo em Português | MEDLINE | ID: mdl-34468661

RESUMO

Little is known regarding the use of emergency contraception among women from different regions of Brazil. The use of emergency contraception as well as contraceptive methods before and after coitus was analyzed. This cross-sectional study assessed the use of emergency contraception by interviewing 2,051 women aged between 18 and 49 attending 76 basic health units in three capitals: São Paulo-SP, Aracaju-SE and Cuiabá-MT. Aspects associated with the use of emergency contraception were analyzed by means of multiple logistic regression. Over half of the women reported the use of emergency contraception (56.7%). Having a high level of education, being from a more privileged socioeconomic group, having a paid job and having had four or more sexual partners were associated with the use of emergency contraception. Being 35 years of age or older and being in a stable relationship was negatively associated. The last time they used emergency contraception, 53.2% used another method, with the male condom and oral pill being the most frequent. Of those who did not use the method, half adopted the regular method after using it (51.7%). The conclusion drawn is that emergency contraception is widely used and does not appear to affect the use of the regular contraceptive method.


Pouco se sabe sobre o uso da anticoncepção de emergência entre mulheres de diferentes regiões do país. Este estudo analisou o uso da anticoncepção de emergência e os aspectos associados, bem como o uso de métodos contraceptivos antes e após. Trata-se de estudo transversal, conduzido com 2.051 mulheres de 18-49 anos, usuárias de 76 Unidades Básicas de Saúde de São Paulo-SP, Aracaju-SE e Cuiabá-MT. Os aspectos associados ao uso da anticoncepção de emergência foram analisados por meio de regressão logística múltipla. Mais da metade das mulheres relatou já ter usado a anticoncepção de emergência (56,7%). Ter alta escolaridade, ser de grupo socioeconômico mais favorecido, ter trabalho remunerado e ter tido quatro ou mais parceiros sexuais associou-se com uso de anticoncepção de emergência. Ter 35 anos de idade ou mais e estar em união estável associou-se negativamente. Da última vez que usaram a anticoncepção de emergência, 53,2% usavam outro método, sendo preservativo masculino e pílula oral os mais frequentes. Das que não usavam método, metade adotou método regular após o uso (51,7%). Conclui-se que a anticoncepção de emergência é amplamente utilizada e parece não contribuir para interrupção do método contraceptivo de uso regular.


Assuntos
Anticoncepção Pós-Coito , Anticoncepcionais Pós-Coito , Adolescente , Adulto , Brasil , Cidades , Comportamento Contraceptivo , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Atenção Primária à Saúde , Adulto Jovem
13.
Mater Sci Eng C Mater Biol Appl ; 128: 112314, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34474865

RESUMO

We used an in situ chemical oxidation method to prepare a new composite of silver nanoparticles (AgNPs) with polypyrrole (PPy), whose properties were optimized through a 23-factorial design of the synthesis conditions. The successful formation of the AgNPs/PPy composite was confirmed by UV-Visible and FTIR spectroscopies. Transmission electron microscopy revealed the presence of AgNPs smaller than 100 nm, dispersed into the PPy matrix. This hybrid composite exhibits a blue fluorescence emission after excitation in the ultraviolet region. In MTT assays, the AgNPs/PPy composite exhibited low cytotoxicity toward non-tumoral cell lines (fibroblast, Vero, and macrophages) and selectively inhibited the viability of HeLa cells. The AgNPs/PPy composite induces ultrastructural changes in HeLa cells that are consistent with the noticeable selectivity exhibited toward them when compared to its action against non-tumoral cell lineages. Also, the AgNPs/PPy exhibited a hemolytic activity below 14% for all blood groups tested, at concentrations up to 125 µg/mL. These results suggest that the AgNPs/PPy composite has a promising potential for use as an antitumoral agent.


Assuntos
Nanopartículas Metálicas , Prata , Antibacterianos , Células HeLa , Humanos , Polímeros , Pirróis/farmacologia
14.
Immunobiology ; 226(1): 152046, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33341661

RESUMO

Although the treatment of chronic Chagas disease (CCD) patients with Benznidazole (Bz) is still controversial, its use may prevent or delay the progression of the disease to the most severe forms. One of the main factors that can influence the effectiveness of the treatment is the possible cooperation between drug effect and the host immune response. Herein, we evaluated the immune response of peripheral blood mononuclear cells (PBMCs) infected with Trypanosoma cruzi and submitted to Bz treatment. Blood samples of CCD patients (n = 7) and non-infected individuals (n = 6) were drawn to obtain PBMCs. After cell culture, the supernatants were harvested and stored, and the cell analyzed by flow cytometer. The results showed that Bz positively regulated the molecular process of cell activation (CD80) and antigen presentation (HLA-DR), increased phagocytosis receptor and macrophage activation (CD64), and did not induce an exacerbated immune response. In conclusion, these results highlight the relevance of using Bz that, despite not being a true hero, it is also not a villain, as it presents a wide range of pharmacological/immunological response interactions, important for the immune balance in the clinical progression of CCD.


Assuntos
Doença de Chagas/imunologia , Leucócitos Mononucleares/imunologia , Nitroimidazóis/farmacologia , Tripanossomicidas/farmacologia , Trypanosoma cruzi/imunologia , Apresentação de Antígeno , Antígeno B7-1/metabolismo , Células Cultivadas , Doença de Chagas/tratamento farmacológico , Doença Crônica , Antígenos HLA-DR/metabolismo , Humanos , Imunidade Celular , Leucócitos Mononucleares/parasitologia , Ativação Linfocitária , Ativação de Macrófagos , Fagocitose
15.
Toxicol Mech Methods ; 31(3): 197-204, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33349088

RESUMO

The thiazole derivative N-1-methyl-2-methyl-pyridine)-N-(p-bromophenylthiazol-2-yl)-hydrazine was used to evaluate the acute oral toxicity in Syrian hamsters. The concentration of the doses (300 mg/kg and 2000 mg/kg) were based on the "Class Acute Toxicity Method" displayed in the OECD-423 guide. In addition, renal and liver biochemical tests were performed, as well as histopathological analysis. Our results showed that the compound's lethal dose (LD50) was 1000 mg/kg and classified as category 4 according to the criteria adopted in the experiment's protocol. Biochemical analysis of the liver function's parameters showed that the LD50 values in all animals were higher than the reference values. However, the analyze of the kidney injury parameters showed an increase in the urea's dosage but a decrease in the albumin's dosage in all animals when compared to the reference values. Kidney biochemical analysis also showed that creatinine's level was only higher than the reference values in one animal. Massive damages in the liver were observed, such as hypertrophy and hyperplasia of the hepatocyte, coagulation necrosis, the presence of mononuclear cells in the sinusoidal capillaries, steatosis, cholestasis, and congestion of sinusoidal capillaries and central-lobular veins. The animals presented renal injuries related to congestion of glomerular and interstitial capillaries, nephrosis of contorted proximal and distal tubules and congestion in the medullary region. In conclusion, the thiazole derivative was well tolerated although it caused acute liver and kidney damages. Therefore, these results showed the need of further investigation of this compound in vivo to evaluate the potential therapeutic effects with chronic models.


Assuntos
Rim , Tiazóis , Animais , Cricetinae , Hidrazinas , Mesocricetus , Piridinas , Tiazóis/toxicidade
17.
BIS, Bol. Inst. Saúde (Impr.) ; 22(1): 16-26, 2021.
Artigo em Português | Sec. Est. Saúde SP, CONASS, SESSP-ISPROD, Sec. Est. Saúde SP | ID: biblio-1399423

RESUMO

O artigo apresenta a discussão dos controles sociais que cercam a prática sexual de adolescentes no Brasil e que visam a contenção da atividade sexual destes indivíduos, principalmente das meninas, e a atualização desses discursos na construção do problema da gravidez na adolescência, colocando-os como objetos de intervenção e de individualização fruto de problemas e responsabilidades femininas, influenciados por uma visão desigual de gênero que desconsidera o desejo feminino. Chama também a atenção para a responsabilidade do Estado e da área de Saúde Pública em democratizar informações e acesso às tecnologias contraceptivas que permitam o direito ao livre exercício da sexualidade deste público, não relacionando a prática com a gravidez, atendendo o interesse que os adolescentes têm enquanto sujeitos de direitos, inclusive de autonomia, com proteção e sigilo de sua intimidade.


Assuntos
Saúde Pública , Adolescente , Anticoncepção , Sexualidade
18.
Artigo em Português | Sec. Est. Saúde SP, CONASS, SESSP-ISPROD, Sec. Est. Saúde SP | ID: biblio-1399417

RESUMO

Fazendo uma revisão dos movimentos sociais femininos e feministas, o artigo chama atenção para as conquistas das mulheres até os direitos sexuais e reprodutivos, como derivados e integrantes de uma luta social em prol das massas, fazendo uma crítica ao abandono dessas ações por um feminismo identitário e neoliberal que vem se estabelecendo na sociedade e que não se preocupa com as questões socioeconômicas que acometem a maioria das mulheres.


Assuntos
Mulheres , Feminismo , Direitos Sexuais e Reprodutivos
19.
BIS, Bol. Inst. Saúde (Impr.) ; 22(2): 76-95, 2021.
Artigo em Português | Sec. Est. Saúde SP, CONASS, SESSP-ISPROD, Sec. Est. Saúde SP | ID: biblio-1399575

RESUMO

Este artigo tem como objetivos descrever os perfis e as atividades de serviços de saúde localizados nos municípios do estado de São Paulo quanto à atenção em saúde sexual e reprodutiva de adolescentes e jovens; e apresentar a orientação para este tema da Linha de Cuidado para a Saúde na Adolescência e Juventude para o Sistema Único de Saúde no Estado de São Paulo, que tem como marco o documento elaborado e apresentado à Comissão Intergestores Bipartite de SP, em 2018, e publicado em livro on-line em 2021.


Assuntos
Adolescente , Assistência Integral à Saúde , Saúde Reprodutiva , Sistema Único de Saúde
20.
BIS, Bol. Inst. Saúde (Impr.) ; 22(2): 125-139, 2021.
Artigo em Português | Sec. Est. Saúde SP, CONASS, SESSP-ISPROD, Sec. Est. Saúde SP | ID: biblio-1399657

RESUMO

Este artigo apresenta o levantamento realizado com serviços da Atenção Básica da Prefeitura de São Paulo durante a entrega de dispensers e folhetos de preservativos femininos pós-licitação no ano de 2019. Profissionais responsáveis pela enfermagem e/ou pelo planejamento reprodutivo relataram os principais espaços e situações de promoção do método contraceptivo no serviço e os comentários espontâneos, positivos e negativos, feitos por colegas de trabalho e pelo público. Também tipificaram a demanda do público, revelando aumento tanto da procura ­ principalmente por mulheres adultas jovens, mas também por adolescentes ­ em grupos, consultas de enfermagem e durante a coleta de papanicolau quanto da retirada por demanda espontânea, além da inserção da oferta do preservativo feminino para pessoas pós-menopausa, idosas e, inclusive, homens.


Assuntos
Saúde , Prevenção de Doenças , Saúde Reprodutiva , Infecções Sexualmente Transmissíveis , Preservativos Femininos
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