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1.
Inorg Chem ; 60(20): 15435-15444, 2021 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-34546735

RESUMO

Coordination polymers have emerged as a new class of potent biologically active agents due to a variety of important characteristics such as the presence of bioactive metal centers and linkers, low toxicity, stability, tailorable structures, and bioavailability. The research on intermediate metabolites has also been explored with implications toward the development of selective anticancer, antimicrobial, and antiviral therapeutic strategies. In particular, quinolinic acid (H2quin) is a recognized metabolite in kynurenine pathway and potent neurotoxic molecule, which has been selected in this study as a bioactive building block for assembling a new silver(I) coordination polymer, [Ag(Hquin)(µ-PTA)]n·H2O (1). This product has been prepared from silver oxide, H2quin, and 1,3,5-triaza-7-phosphaadamantane (PTA), and fully characterized by standard methods including single-crystal X-ray diffraction. Compound 1 has revealed distinctive bioactive features, namely (i) a remarkable antiviral activity against herpes simplex virus type 1 (HSV-1) and adenovirus 36 (Ad-36), (ii) a significant antibacterial activity against clinically important bacteria (Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa), and (iii) a selective cytotoxicity against HeLa (human cervix carcinoma) cell line. The present work widens a growing family of bioactive coordination polymers with potent antiviral, antibacterial, and antiproliferative activity.

2.
Animals (Basel) ; 11(8)2021 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-34438717

RESUMO

Huculs (Equus caballus) are an old breed of primitive mountain horses, originating from the Carpathian Mountains. To the best of our knowledge, data concerning the epidemiology of viral infections observed within this breed are sparse. The objective of this study was to estimate the serological status of a semi-isolated, unvaccinated Hucul herd, with respect to both common equine viral infections and horse-infecting arboviruses, the presence of which was previously reported in Poland. Twenty horses of the Hucul breed, living in a remote area in Poland, were studied in 2018 from March to May. Using nasal secretion swabs as a specimen source, isolation attempts were negative regarding ERAV, EHV-1, EAV, and EIV. According to the virus neutralisation method, in the sera obtained from the animals, antibodies against the following viruses were detected: EHV-1 in 12 horses (60%; with titres from 1:8 to 1:64), EIV A/H7N7 in 13 (65%; titres from 1:20 to 1:80), EIV A /H3N8 in 12 (60%; titres from 1:20 to 1:80), USUV in 5 (25%; titres from 1:10 to 1:80), and ERAV in 1 (5%; titre 1:32). Antibodies against EAV, EIAV, and WNV were not present in the tested sera. The detected presence of specific antibodies associated with five out of the eight equine viruses investigated indicates that the Hucul herd, due to its partial separation and lack of specific prophylaxis, could serve as a sentinel animal group for the detection of equine viruses/arboviruses present within the local ecosystem. The detection of common equine viral infections within the herd provides additional epidemiological data concerning the breed.

3.
Sci Rep ; 11(1): 6639, 2021 03 23.
Artigo em Inglês | MEDLINE | ID: mdl-33758319

RESUMO

Fungi belonging to the Cryptococcus neoformans/C. gattii species complex (CNGSC) are pathogens causing severe infections in humans and animals, that for humans may result in a mortality rate ranging up to 70%. The CNGSC is divided into eight major molecular types, that may differ in their virulence and susceptibility. In order to fully understand the epidemiology of cryptococcosis, it is important to study the world distribution and population structure of these pathogens. The present study is the first presenting a population of strains isolated in Poland and one of the few using a multi-species animal group as a source of the specimen. The pathogen was present in 2.375% of the tested animals. The URA5-RFLP and MALDI-TOF MS analyses have revealed that the population consisted exclusively of C. neoformans strains, with a predominance of major molecular type VNIV (C. neoformans var. neoformans). The MALDI-TOF MS was used to perform the CNGSC strains identification on both the species and sub-species level. Despite the fact that the animals providing the specimens were not treated with 5-fluorocytosine, around 10% of the tested population presented MIC values exceeding 64 mg/L, indicating the existence of the 5-fluorocytosine-resistant strains in the environment.


Assuntos
Doenças dos Animais/epidemiologia , Doenças dos Animais/microbiologia , Criptococose/veterinária , Cryptococcus neoformans/classificação , Doenças dos Animais/história , Animais , Antifúngicos/farmacologia , Cryptococcus neoformans/efeitos dos fármacos , Cryptococcus neoformans/genética , Cryptococcus neoformans/isolamento & purificação , História do Século XXI , Testes de Sensibilidade Microbiana , Tipagem Molecular , Técnicas de Tipagem Micológica , Polônia/epidemiologia , Polimorfismo de Fragmento de Restrição , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
4.
Materials (Basel) ; 12(20)2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31618829

RESUMO

New Ag(I) coordination polymers, formulated as [Ag(µ-PTAH)(NO3)2]n (1) and [Ag(µ-PTA)(NO2)]n (2), were self-assembled as light- and air-stable microcrystalline solids and fully characterized by NMR and IR spectroscopy, electrospray ionization mass spectrometry (ESI-MS(±), elemental analysis, powder (PXRD) and single-crystal X-ray diffraction. Their crystal structures reveal resembling 1D metal-ligand chains that are driven by the 1,3,5-triaza-7-phospaadamantane (PTA) linkers and supported by terminal nitrate or nitrite ligands; these chains were classified within a 2C1 topological type. Additionally, the structure of 1 features a 1D→2D network extension through intermolecular hydrogen bonds, forming a two-dimensional hydrogen-bonded network with fes topology. Furthermore, both products 1 and 2 exhibit remarkable antimicrobial activity against different human pathogen bacteria (S. aureus, E. coli, and P. aeruginosa) and yeast (C. albicans), which is significantly superior to the activity of silver(I) nitrate as a reference topical antimicrobial.

5.
Folia Microbiol (Praha) ; 64(6): 857-860, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30963417

RESUMO

URA5-RFLP is one of the most widely used genotyping methods relating to Cryptococcus neoformans and C. gattii consensus genotype nomenclature. In order to identify a molecular type, this method uses a visual comparison of digested PCR products of tested and reference strains, therefore any anomaly in RFLP patterns of studied isolates makes recognition difficult or impossible. This report describes a strain of VNIV type showing an atypical URA5-RFLP pattern as well as a group of AD hybrids displaying the same anomaly. The atypical RFLP pattern is the result of a point mutation and emergence of a new restriction site. Emergence of the allele presenting a new banding pattern may lead to misidentification using the URA5-RFLP technique; the results of this study as well as the literature data may suggest the spread of the allele in the environment.


Assuntos
Cryptococcus neoformans/genética , Genes Fúngicos/genética , Sequência de Bases , Cryptococcus neoformans/classificação , Microbiologia Ambiental , Genótipo , Mutação , Técnicas de Tipagem Micológica , Orotato Fosforribosiltransferase/genética , Polimorfismo de Fragmento de Restrição
6.
Inorg Chem ; 55(12): 5886-94, 2016 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-27244270

RESUMO

Three new bioactive silver(I) coordination polymers formulated as [Ag2(µ2-PTA)(µ3-PTA)(µ2-pga)(H2O)]n·6H2O (1), [Ag2(µ2-PTA)(µ3-PTA)(Hpmal)2]n·2H2O (2), and [Ag(µ3-PTA) (Hdmga)]n (3) were self-assembled from Ag2O, 1,3,5-triaza-7-phosphaadamantane (PTA), and a substituted dicarboxylic acid (3-phenylglutaric acid (H2pga), phenylmalonic acid (H2pmal), or 3,3-dimethylglutaric acid (H2dmga)) as an ancillary ligand. Compounds 1-3 were fully characterized by IR and NMR spectroscopy, ESI-MS(±), elemental analysis, and single-crystal X-ray diffraction, revealing that their architectural and topological diversity is governed by structural modulation of a dicarboxylate building block. The structures vary from a 1D cyclic chain with the SP 1-periodic net (4,4)(0,2) topology in 2 to distinct 2D metal-organic layers with the cem-d and hcb topologies in 1 and 3, respectively. In addition, compounds 1-3 exhibit a notable antimicrobial efficiency against a panel of common Gram-negative (E. coli and P. aeruginosa) and Gram-positive (S. aureus) bacteria and yeast (C. albicans). The best normalized minimum inhibitory concentrations (normalized MIC) of 11-23 nmol mL(-1) (for bacterial strains) or 68 nmol mL(-1) (for a yeast strain) are shown by compound 2, and the eventual structure-bioactivity correlations are discussed.


Assuntos
Adamantano/análogos & derivados , Anti-Infecciosos/química , Complexos de Coordenação/química , Glutaratos/química , Malonatos/química , Compostos Organofosforados/química , Polímeros/química , Prata/química , Adamantano/química , Anti-Infecciosos/farmacologia , Bactérias/efeitos dos fármacos , Candida albicans/efeitos dos fármacos , Complexos de Coordenação/síntese química , Complexos de Coordenação/farmacologia , Testes de Sensibilidade Microbiana , Análise Espectral/métodos
7.
Chem Biol Drug Des ; 82(5): 579-86, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23841542

RESUMO

Herein, a series of CuI or CuNCS complexes with neocuproine (2,9-dimethyl-1,10-phenanthroline: dmp) and two tris(aminomethyl)phosphines derived from morpholine (P(CH2 N(CH2 CH2 )2 O)3 ) or thiomorpholine (P(CH2 N(CH2 CH2 )2 S)3 ) were tested as cytotoxic agents in vitro towards mouse colon carcinoma (CT26) and human lung adenocarcinoma (A549). The studies showed that the complexes exhibit potential antitumor properties, displayed by IC50 values below 10 µm towards the tested cell lines, in the case of 4-h incubation time with the examined compounds. Moreover, a high antimicrobial activity of all the complexes was observed against Staphylococcus aureus and Candida albicans with minimal inhibitory concentrations equal to 1-2 µg/mL. To gain insight into the molecular mechanism of biological activity of the complexes, we investigated also their interactions with plasmid DNA (pUC18) and the human and bovine serum albumins. Gel electrophoresis experiments demonstrated that all the compounds were comparably efficient in DNA degradation process; however, luminescence quenching showed surprising dependence on the interactions strength of the used compounds with the albumins. Apart from exceptionally effective [CuI(dmp)P(CH2 N(CH2 CH2 )2 O)3 ], the complexes with P(CH2 N(CH2 CH2 )2 O)3 quenched more strongly luminescence of bovine serum albumin, while the complexes with P(CH2 N(CH2 CH2 )2 S)3 were more active in the quenching of human serum albumin luminescence.


Assuntos
Anti-Infecciosos , Complexos de Coordenação , Cobre/química , DNA/metabolismo , Fenantrolinas/química , Fosfinas/química , Albumina Sérica/metabolismo , Animais , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Anti-Infecciosos/toxicidade , Candida albicans/efeitos dos fármacos , Bovinos , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Complexos de Coordenação/química , Complexos de Coordenação/farmacologia , Complexos de Coordenação/toxicidade , DNA/química , Humanos , Camundongos , Testes de Sensibilidade Microbiana , Morfolinas/química , Albumina Sérica/química , Staphylococcus aureus/efeitos dos fármacos
8.
J Vet Diagn Invest ; 23(3): 532-7, 2011 May.
Artigo em Inglês | MEDLINE | ID: mdl-21908285

RESUMO

A set of polymerase chain reaction (PCR) assays for identification of the most important Pasteurellaceae species encountered in cats and dogs were developed. Primers for Pasteurella multocida were designed to detect a fragment of the kmt, a gene encoding the outer-membrane protein. Primers specific to Pasteurella canis, Pasteurella dagmatis, and Pasteurella stomatis were based on the manganese-dependent superoxide dismutase gene (sodA) and those specific to [Haemophilus] haemoglobinophilus on species-specific sequences of the 16S ribosomal RNA gene. All the primers were tested on respective reference and control strains and applied to the identification of 47 canine and feline field isolates of Pasteurellaceae. The PCR assays were shown to be species specific, providing a valuable supplement to phenotypic identification of species within this group of bacteria.


Assuntos
Doenças do Gato/diagnóstico , Doenças do Cão/diagnóstico , Infecções por Pasteurellaceae/veterinária , Pasteurellaceae , Reação em Cadeia da Polimerase/veterinária , Animais , Doenças do Gato/microbiologia , Gatos , Doenças do Cão/microbiologia , Cães , Pasteurella/isolamento & purificação , Infecções por Pasteurella/diagnóstico , Infecções por Pasteurella/microbiologia , Infecções por Pasteurella/veterinária , Pasteurellaceae/isolamento & purificação , Infecções por Pasteurellaceae/diagnóstico , Infecções por Pasteurellaceae/microbiologia , Fenótipo , Polônia
9.
J Antimicrob Chemother ; 66(12): 2784-90, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-21954457

RESUMO

OBJECTIVES: The global occurrence of antibiotic resistance genes in bacteria in water environments is an increasing concern. Treated wastewater was sampled daily over a 45 day period from the outflow of a municipal wastewater treatment plant in Brno, Czech Republic, and examined for extended-spectrum ß-lactamase (ESBL)-producing bacteria. METHODS: Water samples were cultivated on MacConkey agar with cefotaxime (2 mg/L) and individual colonies were examined for ESBL production. Phenotypic ESBL-positive bacteria identified as Escherichia coli or Klebsiella spp. were tested for the presence of antibiotic resistance genes, the virulence gene afa/dra and the bla(CTX-M) upstream region. Genetic relatedness was analysed by PFGE, multilocus sequence typing and plasmid analysis. RESULTS: A total of 68 ESBL-producing Enterobacteriaceae isolates were detected in 34 out of 45 wastewater samples. ESBL-producing isolates included 26 E. coli isolates, 4 Klebsiella pneumoniae isolates and 1 Klebsiella oxytoca isolate. The pandemic and multiresistant B2-O25b-ST131 clone was predominant, being detected among 19 E. coli isolates, and 17 of the B2-O25b-ST131 isolates were positive for the FIA replicon and the afa/dra operon and had an IS26 element flanking bla(CTX-M-15). Seventeen of the B2-O25b-ST131 isolates showed closely related PFGE profiles (defined by 84% band similarity) and belonged to identical clonal groups. CONCLUSIONS: The results highlight the inadequacy of the treatment process in removing multiresistant bacteria from municipal wastewater and point to a risk of transmission of clinically important multiresistant strains, such as the pandemic ST131 clone, to the environment. This is the first study demonstrating the pandemic ST131 clone in wastewater.


Assuntos
Escherichia coli/enzimologia , Escherichia coli/isolamento & purificação , Klebsiella/enzimologia , Klebsiella/isolamento & purificação , Microbiologia da Água , beta-Lactamases/metabolismo , Técnicas Bacteriológicas/métodos , Meios de Cultura/química , República Tcheca , Impressões Digitais de DNA , DNA Bacteriano/genética , Eletroforese em Gel de Campo Pulsado , Escherichia coli/classificação , Escherichia coli/genética , Genótipo , Humanos , Klebsiella/classificação , Klebsiella/genética , Tipagem de Sequências Multilocus , Plasmídeos/análise , Reação em Cadeia da Polimerase , Fatores de Virulência/genética , Purificação da Água
10.
J Inorg Biochem ; 105(8): 1102-8, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21726774

RESUMO

This paper presents the biological activity of copper(I) iodide complexes with 1,10-phenanthroline (phen) or 2,9-dimethyl-1,10-phenanthroline (dmp) and three tris (aminomethyl) phosphanes: P(CH2N(CH2CH2)2NCH3)3 (1), P(CH2N(CH2CH2)2O)3 (2) and P (CH2N(CH3)CH2CH2OH)3 (3). Crystallographic and DFT data indicate a significantly stronger binding ability of 3 in the complexes [CuI (phen) P (CH2N (CH3)CH2CH2OH)3] (3P) and [CuI(dmp)P(CH2N(CH3)CH2CH2OH)3] (3N) in comparison to the 1 or 2 ligands. Most probably, this is caused by the relatively small steric requirements of 3. The complexes with dmp exhibit a very high in vitro activity against the Staphylococcus aureus strain (MIC - minimal inhibitory concentration: 2.5-5 µg/mL) and Candida albicans diploid fungus (MIC: 1.25-2.5 µg/mL). All the tested complexes also show a strong in vitro antitumor activity against human ovarian carcinoma cell lines: MDAH 2774 (IC50: 7-2 µM) and cisplatin-resistant SCOV3 (IC50: 3-2 µM). Interestingly, the complexes with dmp of higher biological activity more weakly interact with bovine serum albumin (BSA) and less efficiently cleave the pBluescriptSK+ plasmid.


Assuntos
Antibacterianos/química , Antifúngicos/química , Antineoplásicos/química , Cobre/química , Iodetos/química , Fosfinas/química , Antibacterianos/farmacologia , Antifúngicos/farmacologia , Antineoplásicos/farmacologia , Candida albicans/efeitos dos fármacos , Linhagem Celular Tumoral , Cobre/farmacologia , Humanos , Iodetos/farmacologia , Ligantes , Fenantrolinas/química , Fenantrolinas/farmacologia , Fosfinas/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Relação Estrutura-Atividade , Água
11.
Curr Microbiol ; 63(1): 87-93, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21573831

RESUMO

A total of 16 Pasteurella dagmatis strains, including 11 feline and 4 canine isolates as well as one strain isolated from a tiger, were analyzed using partial 16S rRNA and rpoB gene sequence comparison. Phylogenetic studies based on both genes revealed that the population of P. dagmatis recovered from cats in Poland differs markedly from canine strains, constituting a well-separated cluster within Pasteurella sensu stricto species group. The isolate from a tiger seems to represent yet another evolutionary lineage within P. dagmatis.


Assuntos
Proteínas de Bactérias/genética , DNA Bacteriano/genética , Variação Genética , Pasteurella/genética , Pasteurella/isolamento & purificação , RNA Ribossômico 16S/genética , Animais , Gatos/microbiologia , Reservatórios de Doenças/microbiologia , Cães/microbiologia , Dados de Sequência Molecular , Pasteurella/classificação , Faringe/microbiologia , Filogenia , Tigres/microbiologia
12.
Pharmacol Biochem Behav ; 98(2): 261-7, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21238473

RESUMO

The aim of this study was to determine the effect of WIN 55,212-2 mesylate (WIN--a non-selective cannabinoid CB1 and CB2 receptor agonist) on the protective action of four classical antiepileptic drugs (carbamazepine, phenytoin, phenobarbital, and valproate) in the mouse maximal electroshock seizure (MES) model. The results indicate that WIN (10 mg/kg, i.p.) significantly enhanced the anticonvulsant action of carbamazepine, phenytoin, phenobarbital and valproate in the MES test in mice. WIN (5 mg/kg) potentiated the anticonvulsant action of carbamazepine and valproate, but not that of phenytoin or phenobarbital in the MES test in mice. However, WIN administered alone and in combination with carbamazepine, phenytoin, phenobarbital and valproate significantly reduced muscular strength in mice in the grip-strength test. In the passive avoidance task, WIN in combination with phenobarbital, phenytoin and valproate significantly impaired long-term memory in mice. In the chimney test, only the combinations of WIN with phenobarbital and valproate significantly impaired motor coordination in mice. In conclusion, WIN enhanced the anticonvulsant action of carbamazepine, phenytoin, phenobarbital and valproate in the MES test. However, the utmost caution is advised when combining WIN with classical antiepileptic drugs due to impairment of motor coordination and long-term memory and/or reduction of skeletal muscular strength that might appear during combined treatment.


Assuntos
Anticonvulsivantes/administração & dosagem , Benzoxazinas/administração & dosagem , Morfolinas/administração & dosagem , Naftalenos/administração & dosagem , Convulsões/prevenção & controle , Animais , Aprendizagem da Esquiva/efeitos dos fármacos , Benzoxazinas/toxicidade , Agonistas de Receptores de Canabinoides , Carbamazepina/administração & dosagem , Sinergismo Farmacológico , Eletrochoque , Masculino , Memória de Longo Prazo/efeitos dos fármacos , Camundongos , Morfolinas/toxicidade , Força Muscular/efeitos dos fármacos , Naftalenos/toxicidade , Fenobarbital/administração & dosagem , Fenitoína/administração & dosagem , Desempenho Psicomotor/efeitos dos fármacos , Convulsões/etiologia , Ácido Valproico/administração & dosagem
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