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1.
J Assist Reprod Genet ; 37(12): 3109-3119, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33034826

RESUMO

PURPOSE: To investigate the association of partial-AZFc deletions in Chilean men with primary spermatogenic failure and their testicular histopathological phenotypes, analyzing the contribution of DAZ dosage, CDY1 copies, and Y-chromosome haplogroups. SUBJECTS AND METHODS: We studied 479 Chilean men: 334 infertile patients with histological examination (233 cases with spermatogenic defects and 101 normal spermatogenesis, obstructive controls, OC), and 145 normozoospermic controls (NC). AZFc subdeletions were detected by single-tagged sequences and single nucleotide variants analysis. DAZ-copy number was quantified by real-time qPCR. Y-chromosome haplogroups (Y-hg) were hierarchically genotyped through 16 biallelic-markers. RESULTS: The prevalence of AZFc-partial deletions was increased in cases (6%) compared with NC (1.4%) (P = 0.035). There was no difference between 143 Sertoli-cell only syndrome, 35 maturation arrest, or 35 mix atrophy patients and controls. However, gr/gr deletions were more frequent in 16 subjects with hypospermatogenesis compared with NC (P = 0.003) and OC (P = 0.013). Y-hg R was the most prevalent (~ 50%), but decreased among gr/gr deletions (21%, P = 0.03). The prevalence of Y-hg M increased in cases versus controls, both in total and non-deleted men (3.9 and 3.7% versus 0.4%, P = 0.009 and P = 0.016, respectively). Among gr/gr deletions, Y-hg H increased compared with non-deleted men (14.3% versus 0.4%, P = 0.0047). CONCLUSION: Partial-AZFc deletions in a Chilean admixed population are associated with secretory azo/oligozoospermia and might have a role in the development of hypospermatogenesis. Low represented haplogroups, Y-hg M and Y-hg H, show an association with the occurrence of spermatogenic failure and gr/gr deletions respectively; however, additional studies are required.

2.
Prev Chronic Dis ; 16: E102, 2019 08 08.
Artigo em Inglês | MEDLINE | ID: mdl-31400100

RESUMO

Preterm birth (<37 weeks gestation) continues to be a significant cause of disease and death in the United States. Its complex causes are associated with several genetic, biological, environmental, and sociodemographic factors. Organizing and visualizing various data that may be related to preterm birth is an essential step for pattern exploration and hypothesis generation and presents an opportunity to increase public and stakeholder involvement. In this article, we describe a collaborative effort to create an online geographic data visualization tool using open software to explore preterm birth in Fresno County, where rates are the highest in California. The tool incorporates information on births, environmental exposures, sociodemographic characteristics, the built environment, and access to care. We describe data sets used to build the tool, the data-hosting platform, and the process used to engage stakeholders in its creation. We highlight an important example of how collaboration can increase the utility of geographic data visualization to improve public health and address health equity in birth outcomes.


Assuntos
Visualização de Dados , Exposição Ambiental , Mapeamento Geográfico , Resultado da Gravidez/epidemiologia , Nascimento Prematuro , Saúde Pública/métodos , California/epidemiologia , Exposição Ambiental/análise , Exposição Ambiental/prevenção & controle , Feminino , Humanos , Recém-Nascido , Colaboração Intersetorial , Vigilância da População/métodos , Gravidez , Nascimento Prematuro/epidemiologia , Nascimento Prematuro/prevenção & controle , Fatores de Risco , Participação dos Interessados
3.
J Appl Oral Sci ; 27: e20180359, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30970114

RESUMO

Amelogenesis imperfecta (AI) is a group of enamel development disorders that alter the structure and chemical composition of the tissue. There is great variability in the clinical presentation; according to Witkop, AI can be categorized into 14 subtypes, which makes its diagnosis extremely complex. OBJECTIVE: This study aimed to describe and determine the frequency of clinical and radiographic features and inheritance patterns found in 41 Chilean families diagnosed with diverse types of AI. MATERIAL AND METHODS: We analyzed the clinical records, photographs, pedigrees and radiographs of 121 individuals recruited between 2003 and 2016. All of the information was included in a database that was analyzed using the application Stata 14. RESULTS: The 72 affected individuals had average age of 16 years, and no sex association with the presence of AI was found. The most frequent clinical subtypes were as follows: 43% hypomature, 25% hypoplastic, 21% hypomature/hypoplastic, 7% hypocalcified and 4% hypocalcified/hypoplastic. The number of severely affected teeth was 22, which occurred in the patients with hypocalcified and hypocalcified/hypoplasic AI who presented the highest number of damaged teeth. Caries and periodontal disease were found in 47 and 32% of the patients, respectively. Malocclusions were observed in 43% of the individuals with AI, with open bite being the most frequent. Radiographically, the thickness of the enamel decreased in 51% of the patients, and 80% showed decreased radiopacity of the enamel compared to that of dentin. Autosomal dominant inheritance pattern was found in 37% of the families with hypoplastic AI, and autosomal recessive pattern was present in 56% of the other clinical subtypes, but more frequently in those affected with hypomature and hypocalcified AI. CONCLUSION: Of the five clinical subtypes, autosomal recessive hypomature, autosomal dominant hypoplastic and autosomal recessive hypomature/hypoplastic AI were the most prevalent subtypes in this group.


Assuntos
Amelogênese Imperfeita/diagnóstico por imagem , Amelogênese Imperfeita/genética , Genealogia e Heráldica , Padrões de Herança , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Amelogênese Imperfeita/epidemiologia , Amelogênese Imperfeita/patologia , Criança , Pré-Escolar , Chile/epidemiologia , Esmalte Dentário/patologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fenótipo , Distribuição por Sexo , Estatísticas não Paramétricas , Adulto Jovem
4.
Colloids Surf B Biointerfaces ; 173: 769-775, 2019 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-30384274

RESUMO

Bacterial inclusion bodies (IBs) were historically considered one of the major obstacles in protein production through recombinant DNA techniques and conceived as amorphous deposits formed by passive and rather unspecific structures of unfolded proteins aggregates. Subsequent studies demonstrated that IBs contained an important quantity of active protein. In this work, we proved that recombinant ß-galactosidase inclusion bodies (IBß-Gal) are functional aggregates. Moreover, they exhibit particular features distinct to the soluble version of the enzyme. The particulate enzyme was highly active against lactose in physiological and in acid pH and also retained its activity upon a pre-incubation at high temperature. IBß-Gal washing or dilution induced the spontaneous release of active enzymes from the supramolecular aggregates. Along this process, we observed a continuous change in the values of several kinetic parameters, including specific activity and Michaelis-Menten constant, measured in the IBß-Gal suspensions. Simultaneously, IBß-Gal turned into a more heterogeneous population where smaller particles appeared. The released protein exhibited secondary structure features more similar to those of the soluble species than to the aggregated enzyme. Concluding, IBß-Gal represents a reservoir and packed source of highly active and stable enzyme.


Assuntos
Proteínas de Escherichia coli/química , Escherichia coli/enzimologia , Corpos de Inclusão/enzimologia , Lactose/química , beta-Galactosidase/química , Clonagem Molecular , Estabilidade Enzimática , Escherichia coli/genética , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Expressão Gênica , Vetores Genéticos/química , Vetores Genéticos/metabolismo , Temperatura Alta , Concentração de Íons de Hidrogênio , Corpos de Inclusão/química , Cinética , Lactose/metabolismo , Agregados Proteicos , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Solubilidade , Relação Estrutura-Atividade , beta-Galactosidase/genética , beta-Galactosidase/metabolismo
5.
J. appl. oral sci ; 27: e20180359, 2019. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-990104

RESUMO

Abstract Amelogenesis imperfecta (AI) is a group of enamel development disorders that alter the structure and chemical composition of the tissue. There is great variability in the clinical presentation; according to Witkop, AI can be categorized into 14 subtypes, which makes its diagnosis extremely complex. Objective: This study aimed to describe and determine the frequency of clinical and radiographic features and inheritance patterns found in 41 Chilean families diagnosed with diverse types of AI. Material and Methods: We analyzed the clinical records, photographs, pedigrees and radiographs of 121 individuals recruited between 2003 and 2016. All of the information was included in a database that was analyzed using the application Stata 14. Results: The 72 affected individuals had average age of 16 years, and no sex association with the presence of AI was found. The most frequent clinical subtypes were as follows: 43% hypomature, 25% hypoplastic, 21% hypomature/hypoplastic, 7% hypocalcified and 4% hypocalcified/hypoplastic. The number of severely affected teeth was 22, which occurred in the patients with hypocalcified and hypocalcified/hypoplasic AI who presented the highest number of damaged teeth. Caries and periodontal disease were found in 47 and 32% of the patients, respectively. Malocclusions were observed in 43% of the individuals with AI, with open bite being the most frequent. Radiographically, the thickness of the enamel decreased in 51% of the patients, and 80% showed decreased radiopacity of the enamel compared to that of dentin. Autosomal dominant inheritance pattern was found in 37% of the families with hypoplastic AI, and autosomal recessive pattern was present in 56% of the other clinical subtypes, but more frequently in those affected with hypomature and hypocalcified AI. Conclusion: Of the five clinical subtypes, autosomal recessive hypomature, autosomal dominant hypoplastic and autosomal recessive hypomature/hypoplastic AI were the most prevalent subtypes in this group.

6.
Rev. Fac. Med. (Guatemala) ; 1(25 Segunda Época): 22-28, Jun - Dic 2018.
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1127679

RESUMO

Introducción: El delirio es una complicación angustiante del uso del Sevoflurano en anestesia general. Este estudio determinó la incidencia del delirio y los factores de riesgo en pacientes en el Hospital Herrera Llerandi. Métodos. Este fue un estudio transversal y observacional que incluyó pacientes pediátricos con edades entre 1 y 12 años, que recibieron anestesia general con Sevoflurano para procedimientos electivos y de urgencia. Resultados: Se incluyeron 159 niños, con una incidencia de 31 (18%) casos de delirio. No existe dependencia entre la edad y género de los niños y su nivel de agitación según la escala postoperatoria. Discusión: Los niños más jóvenes que tienen ansiedad moderada-severa en el período preoperatorio poseen un riesgo mayor de desarrollar delirio pos anestesia general con Sevoflurano. Palabras clave: delirio, Sevofluorano, Hospital Herrera Llerandi, ansiedad


Background: Delirium is a distressing complication with the use of Sevoflurane for general anesthesia. This study determined the incidence of delirium and risk factors in patients at Herrera Llerandi Hospital. Methods: This was a cross-sectional, observational study including pediatric patients aged 1-12 years, undergoing general anesthesia with sevoflurane for elective day-case and urgency procedures. Results: 159 children were included, with an incidence of 31 (18%) cases of delirium. There is no dependence between children's age and gender and their level of agitation according to the postoperative scale. Discussion: Children of younger age with greater preoperative anxiety are at increased risk of developing emergence delirium following general anesthesia with Sevoflurane. Key Words: delirium, Sevoflurane, Hospital Herrera Llerandi, anxiety

7.
Rev. Fac. Med. (Guatemala) ; 1(24 Segunda Época): 1-5, Ene - Jun 2018.
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1049538

RESUMO

Introducción: se ha demostrado que el uso de Ácido Tranexámico (AT) en diferentes procedimientos quirúrgicos puede disminuir la necesidad de transfusiones sanguíneas. Objetivo: Determinar si disminuye la necesidad de transfusiones sanguíneas con el uso de AT en artroplastias de cadera y rodilla. Métodos: Estudio descriptivo y retrospectivo desarrollado con información de 230 pacientes operados en los hospitales Herrera Llerandi y Multimédica en los períodos de 2015, 2016 y 2017. Resultados: Utilizando la distribución binomial para dos poblaciones se obtuvo que existe una disminución de la necesidad de transfusiones sanguíneas del 97.7% al utilizar AT. Los pacientes que no reciben AT tienen una probabilidad de 83.4% de necesitar una transfusión sanguínea. Conclusión: Se logró determinar que con la utilización de Ácido Tranexámico disminuye la necesidad de transfusiones sanguíneas en artroplastias de cadera y rodilla en un 97.7% con el 95% de confianza


Introduction: It has been demonstrated that blood transfusions need is reduced when tranexamic acid (TA) is used during different surgical procedures. Objective: To determine if blood transfusions need is reduced by using TA during total knee and hip arthroplasty. Methods: Descriptive and retrospective study about 230 patients operated in Herrera Llerandi and Multimédica hospitals from 2015 through 2017. Binomial distribution was used for two populations to find out that the probability of reducing the need of blood transfusions Results: The use of blood transfusions was reduced in 97.7% when using TA. The probability of needing a blood transfusion is 83.4% when TA is not used. Conclusion: The need of blood transfusions during total knee and hip arthroplasty is reduced when using tranexamic acid in 97.7% with 95% confidence level.

8.
Transpl Immunol ; 40: 42-50, 2017 02.
Artigo em Inglês | MEDLINE | ID: mdl-27888093

RESUMO

Our goal in using dual induction therapy is to bring the kidney transplant recipient closer (through more effectively timed lymphodepletion) to an optimally immunosuppressed state. Here, we report long-term results of a prospective randomized trial comparing (Group I,N=100) rATG/Dac (3 rATG, 2 Dac doses) vs. (Group II,N=100) rATG/Alemtuzumab(C1H) (1 dose each), using reduced tacrolimus dosing, EC-MPS, and early corticosteroid withdrawal. Lower EC-MPS dosing was targeted in Group II to avoid severe leukopenia. Median follow-up was 96mo post-transplant. There were no differences in 1st BPAR (including borderline) rates: 10/100 vs. 9/100 in Groups I and II during the first 12mo(P=0.54), and 20/100 vs. 20/100 throughout the study(P=0.90). Equally favorable renal function was maintained in both treatment arms(N.S.). While not significant, more patients in Group II experienced graft loss, 25/100 vs. 18/100 in Group I(P=0.23). Actuarial patient/graft survival at 96mo was 92%/83% vs. 85%/73% in Groups I and II(N.S.). DWFG-due-to-infection(N.S.), EC-MPS withholding-due-to-leukopenia during the first 2mo(P=0.03), and incidence of viral infections(P=0.09) were higher in Group II, whereas EC-MPS withholding-due-to-GI symptoms was higher in Group I(P=0.009). No other adverse event differences were observed. While long-term anti-rejection and renal function efficacy were demonstrated in both treatment arms, slight over-immunosuppression of Group II patients occurred.


Assuntos
Alemtuzumab/uso terapêutico , Anticorpos Monoclonais Humanizados/uso terapêutico , Soro Antilinfocitário/uso terapêutico , Rejeição de Enxerto/tratamento farmacológico , Imunoglobulina G/uso terapêutico , Imunossupressores/uso terapêutico , Transplante de Rim , Animais , Daclizumabe , Quimioterapia Combinada , Feminino , Seguimentos , Rejeição de Enxerto/mortalidade , Sobrevivência de Enxerto , Humanos , Imunossupressão , Transplante de Rim/efeitos adversos , Depleção Linfocítica , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Coelhos , Análise de Sobrevida , Resultado do Tratamento
9.
Transpl Int ; 29(2): 216-26, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26442829

RESUMO

The premise that lower TAC trough levels are associated with subsequently higher first BPAR risk during the first 12 mo post-transplant was recently questioned. Using our prospectively followed cohort of 528 adult, primary kidney transplant recipients (pooled across four randomized trials) who received reduced TAC dosing plus an IMPDH inhibitor, TAC trough levels measured at seven time points, 7, 14 days, 1, 2, 3, 6 and 9 months post-transplant, were utilized along with Cox's model to determine the multivariable significance of TAC level(t) (a continuous time-dependent covariate equaling the most recently measured TAC level prior to time t) on the hazard rate of developing first BPAR during the first 12 months post-transplant. The percentage developing BPAR during the first 12 months post-transplant was 10.2% (54/528). In univariable analysis, lower TAC level(t) was associated with a significantly higher BPAR rate (P = 0.00006), and its significance was maintained even after controlling for 2 significant baseline predictors (African-American/Hispanic Recipient and Developed DGF) in Cox's model (multivariable P = 0.0003). Use of a cutpoint, TAC level(t) <4.0 vs. ≥4.0 ng/ml, yielded an even greater association with BPAR rate (univariable and multivariable P < 0.000001), with an estimated hazard ratio of 6.33. These results suggest that TAC levels <4.0 ng/ml should be avoided during the first 12 months post-transplant when TAC is used in combination with fixed-dose mycophenolate with or without corticosteroids and induction therapy.


Assuntos
Rejeição de Enxerto/etiologia , Imunossupressores/farmacocinética , Transplante de Rim/efeitos adversos , Tacrolimo/farmacocinética , Doença Aguda , Adulto , Idoso , Função Retardada do Enxerto/etiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Modelos de Riscos Proporcionais , Estudos Prospectivos , Fatores de Tempo
10.
Ginecol Obstet Mex ; 83(9): 522-8, 2015 Sep.
Artigo em Espanhol | MEDLINE | ID: mdl-26591040

RESUMO

BACKGROUND: Breast cancer is the leading cause of death from cancer in the female population; consequently, there are multiple prevention campaigns. Within these campaigns, a special emphasis is given on promoting monthly breast self-examination; however, many women have never received formal education on proper method of self-examination. OBJECTIVE: To establish if the educational intervention we propose improves the breast self-examination technique. MATERIAL AND METHODS: A descriptive longitudinal study that included 52 women aged 20-40 years, attending a Family Medicine Unit of the Mexican Institute of Social Security, who were evaluated about self-examination technique before and after educational intervention, measured on a scale of 0 to 16. Statistical analysis was made with descriptive statistics and Student's t test. RESULTS: The mean age was 30.76 ± 5.87 years. The mean baseline score was 3.13 ± 2.55. The final average score after a month of the educational intervention was 10.69 ± 2.74, which represents an increase in average score of 7.55 ± 3.53. There was a significant increase in assessment scores after the educational intervention (p < 0.001). CONCLUSIONS: "Supervised breast self-examination" technique showed an increase in the ability of self-examination in patients. It can be considered an effective complementary method of teaching breast self-examination.


Assuntos
Autoexame de Mama , Adulto , Neoplasias da Mama/prevenção & controle , Autoexame de Mama/normas , Feminino , Educação em Saúde/métodos , Humanos , Estudos Longitudinais , Adulto Jovem
11.
Clin Transplant ; 29(4): 301-10, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25581205

RESUMO

BACKGROUND: Understanding the relative contributions of baseline demographics and immunosuppressive therapy on NODAT risk may help in developing preventive strategies. METHODS: Using our prospectively followed cohort of 481 adult, primary kidney transplant recipients without pre-transplant diabetes, we determined the significant baseline predictors for the hazard rate of developing NODAT via Cox stepwise regression. The multivariable influence of first BPAR (defined as a time-dependent covariate) was also tested. RESULTS: Median follow-up was 57 mo post-transplant; the overall percentage who developed NODAT was 22.5% (108/481). Four baseline predictors of a greater NODAT hazard rate were found (by order of selection): higher BMI (p < 0.000001), planned maintenance with SRL (p = 0.0003), non-white recipient (p = 0.0004), and older recipient age (p = 0.0004). Approximately one-half of the 106 patients in the highest demographic risk category (BMI ≥25 kg/m(2) , non-white race, and age at transplant ≥40 yr) developed NODAT; actuarial NODAT risk ranged from 10% to 30% in the lower demographic risk categories. First BPAR was also associated with significantly higher NODAT in multivariable analysis (p = 0.02)-the highly elevated NODAT rate observed during the first few months post-transplant and following first BPAR appears to demonstrate the diabetogenic effect of using high-dose (intravenous) corticosteroids. CONCLUSIONS: The disturbingly high NODAT rate found among patients having multiple demographic risk factors is still an important problem that awaits a better solution.


Assuntos
Diabetes Mellitus/etiologia , Falência Renal Crônica/cirurgia , Transplante de Rim/efeitos adversos , Complicações Pós-Operatórias , Adulto , Fatores Etários , Feminino , Seguimentos , Taxa de Filtração Glomerular , Rejeição de Enxerto/tratamento farmacológico , Rejeição de Enxerto/etiologia , Humanos , Imunossupressores/uso terapêutico , Testes de Função Renal , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Prospectivos , Fatores de Risco , Transplantados
12.
Diabetologia ; 58(2): 334-45, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25361829

RESUMO

AIMS/HYPOTHESIS: To better understand the implications of new-onset diabetes after transplant (NODAT), we used our prospectively followed cohort of 628 adult primary kidney transplant recipients to determine the prognostic impact of pretransplant diabetes and NODAT. METHODS: The study cohort consisted of all participants in four randomised immunosuppression trials performed at our centre since May 2000. For each cause-specific hazard analysed, Cox stepwise regression was used to determine a multivariable model of significant baseline predictors; the multivariable influence of having pretransplant diabetes and NODAT (t) (the latter defined as a zero-one, time-dependent covariate) was subsequently tested. Similar analyses of estimated glomerular filtration rate (eGFR) at 36 and 60 months post transplant were performed using stepwise linear regression. Finally, a repeated measures analysis of mean HbA1c as a function of diabetes category (pretransplant diabetes vs NODAT) and randomised trial (first to fourth) was performed. RESULTS: Median follow-up was 56 months post transplant. Patients with pretransplant diabetes comprised 23.4% (147/628), and 22.5% (108/481) of the remaining patients developed NODAT. Pretransplant diabetes had no prognostic influence on first biopsy-proven acute rejection and death-censored graft failure hazard rates, nor on eGFR, but was associated with significantly higher rates of death with a functioning graft (DWFG) (p = 0.003), DWFG due to a cardiovascular event (p = 0.005) and infection that required hospitalisation (p = 0.03). NODAT (t) had no unfavourable impact on any of these hazard rates nor on eGFR, with actuarial freedom from DWFG remaining at over 90% among patients in pre- and post-NODAT states at 72 months post transplant/NODAT. Mean HbA1c for patients in the first to fourth randomised trials, averaged across diabetes category, decreased by trial (7.28%, 6.92%, 6.87% and 6.64% [56.1, 52.1, 51.6 and 49.1 mmol/mol], respectively; p = 0.02). CONCLUSIONS/INTERPRETATION: Less-than-expected post-NODAT risk for graft loss and death may exist in the current climate of tighter glucose monitoring post transplant.


Assuntos
Diabetes Mellitus/etiologia , Transplante de Rim/efeitos adversos , Transplantados/estatística & dados numéricos , Estudos de Coortes , Diabetes Mellitus/sangue , Diabetes Mellitus/mortalidade , Feminino , Seguimentos , Rejeição de Enxerto , Sobrevivência de Enxerto , Humanos , Imunossupressores , Transplante de Rim/mortalidade , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Prospectivos , Fatores de Risco
13.
Artif Cells Nanomed Biotechnol ; 43(1): 40-9, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24047541

RESUMO

Abstract NIPA and HEMA-lactate-Dextran-based biodegradable and thermoresponsive cryogels were synthesized at different compositions by cryogelation. Chemical and morphological properties of the HEMA-lactate-Dextran-co-NIPA cryogel matrices were demonstrated by FTIR, SEM, and ESEM. Thermoresponsivity of the prepared cryogels was investigated by DSC, imaging NMR, and swelling studies. For possible use of the cryogels in potential bone tissue engineering applications, either hydrophobic simvastatin was embedded, or hydrophilic simvastatin was incorporated in the cryogels. Release profiles of simvastatin delivering cryogel scaffolds depending on their composition, hydrophobicity or hydrophilicity of loaded simvastatin and the medium temperature were demonstrated.


Assuntos
Anticolesterolemiantes/química , Criogéis/química , Preparações de Ação Retardada/química , Polímeros/química , Sinvastatina/química , Acrilamidas/química , Dextranos/química , Composição de Medicamentos , Humanos , Interações Hidrofóbicas e Hidrofílicas , Cinética , Ácido Láctico/química , Metacrilatos/química , Microscopia Eletrônica de Varredura , Porosidade , Soluções , Espectroscopia de Infravermelho com Transformada de Fourier , Temperatura
14.
Braz J Microbiol ; 45(3): 1095-9, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25477948

RESUMO

Edwarsiella tarda is a zoonotic bacterium that can be isolated from humans, animals and the environment. Although E. tarda is primarily considered a fish pathogen, it is the only species of its genus considered to be pathogenic for humans as well. A survey of zoonotic intestinal bacteria in fresh feces from South American sea lions (SASL) Otaria flavescens, reported E. tarda as the most frequently isolated species. In this study, we used HEp-2 cells to establish in vitro the adherence and invasive ability of 17 E. tarda strains isolated from SASL fecal material. All the strains were able to adhere and invade HEp-2 cells with adhesion and invasion percentages ranging from 56 to 100% and 21 to 74%, respectively. Despite the expression of these pathogenic factors, further investigation is needed to determine whether this bacterium could play a role as primary pathogen for this and other species of pinnipeds.


Assuntos
Aderência Bacteriana , Edwardsiella tarda/fisiologia , Endocitose , Infecções por Enterobacteriaceae/veterinária , Hepatócitos/microbiologia , Leões-Marinhos/microbiologia , Animais , Edwardsiella tarda/isolamento & purificação , Infecções por Enterobacteriaceae/microbiologia , Células Hep G2 , Humanos , América do Sul
15.
Braz. j. microbiol ; 45(3): 1095-1099, July-Sept. 2014. ilus, tab
Artigo em Inglês | LILACS | ID: lil-727043

RESUMO

Edwarsiella tarda is a zoonotic bacterium that can be isolated from humans, animals and the environment. Although E. tarda is primarily considered a fish pathogen, it is the only species of its genus considered to be pathogenic for humans as well. A survey of zoonotic intestinal bacteria in fresh feces from South American sea lions (SASL) Otaria flavescens, reported E. tarda as the most frequently isolated species. In this study, we used HEp-2 cells to establish in vitro the adherence and invasive ability of 17 E. tarda strains isolated from SASL fecal material. All the strains were able to adhere and invade HEp-2 cells with adhesion and invasion percentages ranging from 56 to 100% and 21 to 74%, respectively. Despite the expression of these pathogenic factors, further investigation is needed to determine whether this bacterium could play a role as primary pathogen for this and other species of pinnipeds.


Assuntos
Animais , Humanos , Aderência Bacteriana , Endocitose , Edwardsiella tarda/fisiologia , Infecções por Enterobacteriaceae/veterinária , Hepatócitos/microbiologia , Leões-Marinhos/microbiologia , Edwardsiella tarda/isolamento & purificação , Infecções por Enterobacteriaceae/microbiologia , América do Sul
16.
Transplantation ; 97(9): 925-33, 2014 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-24445926

RESUMO

BACKGROUND: In adult kidney transplantation, there is no clear consensus on the incidence of graft failure-due-to noncompliance (GFNC), with some reporting it as relatively uncommon and others as a major cause of late graft failure. We suspected that GFNC was a major cause of late graft loss at our center but did not know the extent of this problem. METHODS: In our prospectively followed cohort of 628 adult, primary kidney-alone transplant recipients with long-term follow-up, GFNC and other graft loss causes were determined from our ongoing clinical evaluations. Using competing risks methodology, we determined the overall percentage of patients developing GFNC and the significant prognostic factors for its hazard rate and cumulative incidence (via Cox regression). RESULTS: Cumulative incidence estimates (± standard error) of GFNC (n=29), GF-with-compliance (n=46), receiving a never-functioning graft (n=7), and death-with-a-functioning-graft (n=53) at 101 months after transplant (last-observed-graft loss) were as follows: 9.8%± 2.4%, 10.9%± 1.7%, 1.1%± 0.4%, and 13.0%± 1.9%, respectively. Only three patients experienced GFNC during the first 24 months; GFNC represented 48.1% (26/54) of death-censored GFs beyond 24 months. Two baseline variables were jointly associated with a significantly higher GFNC hazard and cumulative incidence: younger recipient age (P<0.000001 each) and non-white recipient (P=0.004 and P=0.02). Estimated percentages of ever developing GFNC were 28.4%± 6.5% among 79 non-whites younger than 35 years versus 0.0% (0/144) among whites 50 years or older. Among 302 recipients younger than 50 years, 18.1%± 4.1% developed GFNC, representing 67.6% (25/37) of its death-censored graft failures observed beyond 24 months after transplant. CONCLUSIONS: GFNC is a major cause of late GF at our center, with younger and non-white recipients at a significantly greater GFNC risk. Interventional approaches to eliminate GFNC could dramatically improve long-term kidney graft survival.


Assuntos
Rejeição de Enxerto , Transplante de Rim , Adesão à Medicação , Insuficiência Renal/terapia , Adulto , Fatores Etários , Feminino , Seguimentos , Humanos , Imunossupressores/uso terapêutico , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Prognóstico , Modelos de Riscos Proporcionais , Estudos Prospectivos , Ensaios Clínicos Controlados Aleatórios como Assunto , Análise de Regressão , Insuficiência Renal/etnologia , Risco , Resultado do Tratamento
17.
Transplantation ; 97(11): 1128-38, 2014 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-24477186

RESUMO

BACKGROUND: In searching for an optimal induction regimen, we conducted two separate randomized trials of 38 living donor and 90 deceased donor adult, primary kidney transplant recipients comparing antithymocyte globulin (Thymoglobulin) (group A, N=43) versus alemtuzumab (group B, N=43) versus daclizumab (group C, N=42), using exactly the same three treatment arms in each trial. METHODS: For the purpose of maximizing statistical power, results from the two randomized trials were combined. Groups A and C received standard maintenance dosing with tacrolimus (TAC), mycophenolate mofetil (MMF), and corticosteroids. Because of intense lymphodepletion expected with alemtuzumab use (and hoped-for achievement of a truer immunoregulatory state), group B received lower TAC and MMF dosing and corticosteroid avoidance. Long-term target TAC trough level and MMF dosing were 5 to 7 ng/mL and 1,000 mg b.i.d. in groups A and C; 4 to 6 ng/mL and 500 mg b.i.d. in group B. RESULTS: With median follow-up of 95 months, biopsy-proven cute rejection incidence was similar in the three groups (8/43, 14/43, and 12/42, P=0.34), but biopsy-proven chronic allograft injury incidence was significantly higher in group B (19/43) in comparison with groups A (9/43) and C (7/42) combined (P=0.0008). Mean calculated creatinine clearance was significantly lower in group B versus the average of groups A and C means throughout 60 months posttransplant (62.9±4.2 vs. 83.6±6.9 and 79.8±5.9 at 60 months, P=0.01), and death-censored graft failure was significantly higher in group B (13/43) versus groups A (5/43) and C (5/42) combined (P=0.009). Total infection and new-onset diabetes after transplant rates were not significantly different. Ad hoc analysis suggested that the inferior results in group B were specifically a result of reduced dosing and greater withholding of TAC and MMF occurring in that group. CONCLUSIONS: Long-term results clearly indicate inferior clinical outcomes in group B.


Assuntos
Anticorpos Monoclonais/química , Transplante de Rim/métodos , Corticosteroides/administração & dosagem , Adulto , Alemtuzumab , Anticorpos Monoclonais Humanizados/administração & dosagem , Soro Antilinfocitário/administração & dosagem , Cadáver , Creatinina/sangue , Daclizumabe , Feminino , Seguimentos , Rejeição de Enxerto , Sobrevivência de Enxerto/imunologia , Humanos , Imunoglobulina G/administração & dosagem , Imunossupressores/administração & dosagem , Doadores Vivos , Masculino , Pessoa de Meia-Idade , Ácido Micofenólico/administração & dosagem , Ácido Micofenólico/análogos & derivados , Tacrolimo/administração & dosagem , Resultado do Tratamento
18.
Nat Methods ; 10(11): 1063-7, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-24173381

RESUMO

Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.


Assuntos
Serviços de Informação , Reação em Cadeia da Polimerase/métodos , Coleta de Dados
19.
Mol Cancer ; 12(1): 127, 2013 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-24148564

RESUMO

Malignant tumors of the central nervous system (CNS) are the 10th most frequent cause of cancer mortality. Despite the strong malignancy of some such tumors, oncogenic mutations are rarely found in classic members of the RAS family of small GTPases. This raises the question as to whether other RAS family members may be affected in CNS tumors, excessively activating RAS pathways. The RAS-related subfamily of GTPases is that which is most closely related to classical Ras and it currently contains 3 members: RRAS, RRAS2 and RRAS3. While R-RAS and R-RAS2 are expressed ubiquitously, R-RAS3 expression is restricted to the CNS. Significantly, both wild type and mutated RRAS2 (also known as TC21) are overexpressed in human carcinomas of the oral cavity, esophagus, stomach, skin and breast, as well as in lymphomas. Hence, we analyzed the expression of R-RAS2 mRNA and protein in a wide variety of human CNS tumors and we found the R-RAS2 protein to be overexpressed in all of the 90 CNS cancer samples studied, including glioblastomas, astrocytomas and oligodendrogliomas. However, R-Ras2 was more strongly expressed in low grade (World Health Organization grades I-II) rather than high grade (grades III-IV) tumors, suggesting that R-RAS2 is overexpressed in the early stages of malignancy. Indeed, R-RAS2 overexpression was evident in pre-malignant hyperplasias, both at the mRNA and protein levels. Nevertheless, such dramatic changes in expression were not evident for the other two subfamily members, which implies that RRAS2 is the main factor triggering neural transformation.


Assuntos
Neoplasias Encefálicas/metabolismo , Glioma/metabolismo , Proteínas de Membrana/metabolismo , Proteínas Monoméricas de Ligação ao GTP/metabolismo , Adolescente , Adulto , Idoso , Feminino , Expressão Gênica , Humanos , Masculino , Proteínas de Membrana/genética , Pessoa de Meia-Idade , Proteínas Monoméricas de Ligação ao GTP/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Análise Serial de Tecidos , Adulto Jovem
20.
Rev Argent Microbiol ; 45(2): 75-9, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23876267

RESUMO

We investigated the possibility of enhancing the adherence capacity of four low-adherent Arcobacter butzleri strains after serial intraperitoneal passage (i.p.) in mice. All the strains enhanced their adherence capacity after the first passage, increasing their adhesion rates after each passage. These results suggest that i.p. passage enhances the expression of adherence in A. butzleri strains.


Assuntos
Arcobacter/fisiologia , Aderência Bacteriana , Animais , Camundongos , Peritônio , Inoculações Seriadas
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