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1.
Mol Biol (Mosk) ; 55(4): 598-605, 2021.
Artigo em Russo | MEDLINE | ID: mdl-34432777

RESUMO

Recently, a wealth of data have been accumulating on the role of long non-coding RNAs (lncRNAs) in the fine-tuning of mRNA expression. Four new lncRNAs, namely, TMEM92-AS1, FAM222A-AS, TXLNB, and lnc-CCL28, were identified as differentially expressed in ovarian tumors using deep machine learning. The levels of lnc-CCL28 transcripts in both tumors and normal tissue samples were sufficient for further analysis by RT-PCR. In addition, the promising ovarian cancer biomarkers, lncRNAs LINC00152, NEAT 1 and SNHG17 were added to RT-PCR analysis. For the first time, an increase in the level of lnc-CCL28 and SNHG 17 lncRNAs was found in ovarian tumors, and the overexpression of LINC00152 and NEAT1 was confirmed. It seems that lnc-CCL28 is involved in carcinogenesis and, in particular, in ovarian cancer progression. Overexpression of LINC00152 and lnc-CCL28 was significantly associated with the later stages and metastasis.


Assuntos
Neoplasias Ovarianas , RNA Longo não Codificante , Carcinogênese/genética , Feminino , Humanos , Neoplasias Ovarianas/genética , RNA Longo não Codificante/genética
2.
Bull Exp Biol Med ; 171(3): 370-374, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34292442

RESUMO

The role of methylation in the regulation of genes of long noncoding RNA (lncRNA) is still poorly understood. We revealed new hypermethylated lncRNA genes in ovarian tumors and their effect on metastasis of ovarian cancer. A multiple and significant (p<0.001) increase in methylation of a group of lncRNA genes (MEG3, SEMA3B-AS1, ZNF667-AS1, and TINCR) was shown by quantitative methylation-specific PCR using the non-parametric Mann-Whitney test. Moreover, methylation of SEMA3B-AS1, ZNF667-AS1, and TINCR genes in ovarian cancer tumors was detected for the first time. Comparative analysis of 19 samples of peritoneal metastases and paired primary tumors showed a significant decrease in the methylation level of the same 4 genes: MEG3 (p=0.004), SEMA3B-AS1 (p=0.002), TINCR (p=0.002), and ZNF667-AS1 (p<0.001). Reduced methylation of suppressor lncRNA genes in peritoneal metastases is probably associated with the involvement of these lncRNA in the regulation of plastic reversion of the epithelial-mesenchymal transition to the mesenchymal-epithelial transition. Thus, the effect of lncRNA and their methylation on the development of tumors and metastases of ovarian cancer was demonstrated, which is important for understanding of the pathogenesis and mechanisms of metastasis of ovarian cancer. New properties of lncRNA can find application in the development of new approaches in the therapy of ovarian cancer.

3.
Mol Biol (Mosk) ; 54(5): 776-801, 2020.
Artigo em Russo | MEDLINE | ID: mdl-33009789

RESUMO

It was more than twenty years ago that miRNAs were recognized as a new class of RNA, but the understanding of their regulatory role is just beginning to emerge. Furthermore, it was found that the function of miRNAs as "master regulators" can be controlled by other non-coding RNAs (ncRNAs), in particular, long ncRNAs (lncRNAs). The regulatory functions of lncRNAs have been indicated in tumors in various locations and, in particular, in osteosarcoma, the most common and most aggressive malignant bone disease in children during puberty. This review discusses studies about the role of lncRNAs in the regulation of gene expression by the competitive endogenous RNAs (ceRNAs) mechanism. Data from these publications confirm the involvement of lncRNAs in the major signaling pathways, such as Notch, PI3K/AKT, Wnt/ß-catenin, JNK, and HIV/VEGF. For example, seven members of the SNHG family (small nucleolar RNA host gene) were shown to participate in the Notch and PI3K/AKT signaling pathways; moreover, several lncRNA/miRNA/mRNA regulatory axes were identified for nearly all members of this family. The functions of other multifunctional oncogenic lncRNAs are also discussed; in particular, six to ten such axes have been determined for TUG1, MALAT1, and XIST. Using the Gene Cards, KEGG, and Panther databases, the key signaling pathways were identified for the targets of these three multifunctional lncRNAs. Investigation of lncRNA function contributes to the development of new diagnostic and prognostic markers for the treatment of patients with osteosarcoma. According to the available data, interactions between ceRNAs, that is, miRNAs, mRNAs, and lncRNAs, represent a new form of gene expression regulation that is involved in various pathophysiological processes, including bone oncogenesis.


Assuntos
Neoplasias Ósseas , Osteossarcoma , RNA Longo não Codificante , Neoplasias Ósseas/genética , Redes Reguladoras de Genes , Humanos , Osteossarcoma/genética , RNA Longo não Codificante/genética , Transdução de Sinais
4.
Bull Exp Biol Med ; 168(3): 366-370, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31938917

RESUMO

Systems of markers for the diagnosis of breast cancer based on DNA methylation of a group of suppressor protein-coding genes, hypermethylated microRNA genes, and their combinations were compiled. On a representative sample of 70 paired breast cancer specimens (tumor/normal), MS-PCR analysis revealed a significant increase in the methylation frequency of 5 protein-coding genes: RASSF1A suppressor and apoptosis genes APAF1, BAX, BIM/BCL2L11, and DAPK1 (34-61% vs. 4-24%) and 6 microRNA genes: MIRG124G1, MIRG125bG1, MIRG129G2, MIRG148a, MIRG34b/c, and MIRG9G3 (36-76% vs. 6-27%). ROC-analysis showed that a combination of 4 genes (APAF1, BAX, BIM/BCL2L11, and DAPK1) and MIRG125bG1 gene constitute a highly efficient 5-marker system with 100% specificity and sensitivity of 94-96% at AUC=0.98-0.97, suitable also for patients with stage I and II breast cancer. Detection of methylation of at least one gene in this system in biopsy or postoperative material is sufficient to refer the sample to breast cancer.


Assuntos
Neoplasias da Mama/diagnóstico , Neoplasias da Mama/genética , MicroRNAs/metabolismo , Fator Apoptótico 1 Ativador de Proteases/genética , Proteína 11 Semelhante a Bcl-2/genética , Metilação de DNA/genética , Metilação de DNA/fisiologia , Proteínas Quinases Associadas com Morte Celular/genética , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Técnicas In Vitro , Espectrometria de Massas , Reação em Cadeia da Polimerase , Proteínas Supressoras de Tumor/genética
5.
Bull Exp Biol Med ; 168(2): 280-284, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31782000

RESUMO

Groups of microRNA genes, methylation of which is associated with the initial (I-II) stages of breast cancer, are determined, and new markers and marker systems for the disease diagnosis were created on the basis of these data. A total of 14 genes in which methylation was associated with breast cancer were identified with the use of methyl-specific PCR on a representative sample of 70 tumor specimens. Analysis of 46 specimens from patients with clinical stages I and II detected 9 genes (MIR-124-1, MIR-124-3, MIR-125b-1, MIR-129-2, MIR-132, MIR-148a, MIR-193a, MIR-34b/c, and MIR-9-3), in which methylation was associated with the initial stages of the disease. Using ROC analysis, we formed two systems including 6 markers each and detecting breast cancer at stages I-II with high sensitivity (89 and 91%) and specificity (88%) at AUC=0.92-0.93. These sets were validated on the total sample of 70 specimens including all disease stages; they showed 93 and 94% sensitivities, 88% specificity, and AUC=0.95. Highly sensitive systems of markers, based on microRNA gene methylation, were created for the diagnosis of breast cancer at stages I-II.


Assuntos
Biomarcadores Tumorais/genética , Neoplasias da Mama/diagnóstico , Neoplasias da Mama/genética , Metilação de DNA/genética , MicroRNAs/genética , Progressão da Doença , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos
6.
Bull Exp Biol Med ; 167(1): 79-83, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31177462

RESUMO

We identified a group of miRNA genes whose methylation is associated with ovarian cancer metastasis. Based on these data, new markers and the systems of markers predicting tumor dissemination were selected. Using methylation-specific PCR and a representative set of 54 ovarian cancer samples, we identified 10 microRNA genes (MIR-124a-2, MIR-127, MIR-125b-1, MIR-129-2, MIR-137, MIR-193a, MIR-203a, MIR-34b/c, MIR-130b, and MIR-1258) whose methylation is associated with tumor metastasis. The greatest association was established for 4 genes: MIR-137, MIR-193a, MIR-34b/c, and MIR-130b (p<0.01). ROC analysis revealed 3 most optimal marker systems including 4-5 miRNA genes and characterized by high sensitivity (82-94%) and specificity (76-86%) at AUC=0.89-0.92. Methylation of any three genes from these systems is sufficient to predict metastasis with the specified accuracy. Detection of the group of hypermethylated miRNA genes with predictive value for ovarian cancer metastasis is of great importance for personalized treatment of the patients.


Assuntos
Metilação de DNA/genética , MicroRNAs/genética , Neoplasias Ovarianas/genética , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Reação em Cadeia da Polimerase
7.
Biochemistry (Mosc) ; 82(5): 529-541, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28601063

RESUMO

Metastasis of primary tumors progresses stepwise - from change in biochemistry, morphology, and migratory patterns of tumor cells to the emergence of receptors on their surface that facilitate directional migration to target organs followed by the formation of a specific microenvironment in a target organ that helps attachment and survival of metastatic cells. A set of specific genes and signaling pathways mediate this process under control of microRNA. The molecular mechanisms underlying biological processes associated with tumor metastasis are reviewed in this publication using ovarian cancer, which exhibits high metastatic potential, as an example. Information and data on the genes and regulatory microRNAs involved in the formation of cancer stem cells, epithelial-mesenchymal transition, reducing focal adhesion, degradation of extracellular matrix, increasing migration activity of cancer cells, formation of spheroids, apoptosis, autophagy, angiogenesis, formation of metastases, and development of ascites are presented. Clusters of microRNAs (miR-145, miR-31, miR-506, miR-101) most essential for metastasis of ovarian cancer including the families of microRNAs (miR-200, miR-214, miR-25) with dual role, which is different in different histological types of ovarian cancer, are discussed in detail in a section of the review.


Assuntos
Movimento Celular , Transição Epitelial-Mesenquimal , MicroRNAs/metabolismo , Células-Tronco Neoplásicas/metabolismo , Neoplasias Ovarianas/metabolismo , RNA Neoplásico/metabolismo , Animais , Feminino , Humanos , MicroRNAs/genética , Metástase Neoplásica , Células-Tronco Neoplásicas/patologia , Neoplasias Ovarianas/genética , Neoplasias Ovarianas/patologia , RNA Neoplásico/genética
8.
Mol Biol (Mosk) ; 51(1): 73-84, 2017.
Artigo em Russo | MEDLINE | ID: mdl-28251969

RESUMO

Methylation of promoter CpG islands and microRNA (miRNA) interactions with mRNAs of target genes are epigenetic mechanisms that play a crucial role in deregulation of gene expression and signaling pathways in tumors. Altered expression of six chromosome 3p genes (RARB(2), SEMA3B, RHOA, GPX1, NKIRAS1, and CHL1) and two miRNA genes (MIR-129-2 and MIR-9-1) was observed in primary clear cell renal cell carcinomas (ccRCCs, 31-48 samples) by RT-PCR and qPCR. Significant downregulation (p < 0.05, Fisher's exact test) was observed for SEMA3B, NKIRAS1, and CHL1; and differential expression, for the other chromosome 3p and miRNA genes. Methylation-specific PCR with primers to RARB(2), SEMA3B, MIR-129-2, and MIR-9-1 showed that their methylation frequency was significantly (p < 0.05, Fisher's exact test) elevated in the ccRCC samples. Significant correlations between promoter methylation and expression were confirmed for SEMA3B and observed for the first time for RARB(2), GPX1, and MIR-129-2 in ccRCC (Spearman's correlation coefficient rs ranging 0.31-0.60, p < 0.05). The MIR-129-2 and RARB(2) methylation frequencies significantly correlated with ccRCC progression. MIR-129-2 methylation correlated with upregulation of RARB(2), RHOA, NKIRAS1, and CHL1 (rs ranging 0.35-0.53, p < 0.05). The findings implicate methylation in regulating RARB(2), SEMA3B, GPX1, and MIR-129-2 and indicate that miR-129-2 and methylation of its gene affect RARB(2), RHOA, NKIRAS1, and CHL1 expression.


Assuntos
Carcinoma de Células Renais/genética , Metilação de DNA , Neoplasias Renais/genética , MicroRNAs/genética , Linhagem Celular Tumoral , Regulação Neoplásica da Expressão Gênica , Humanos , Regiões Promotoras Genéticas
9.
Biochemistry (Mosc) ; 81(4): 315-28, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27293089

RESUMO

This review summarizes data on microRNA (miRNA) genomic organization, biogenesis, and functions in carcinogenesis. The roles of key genes and regulatory miRNAs in molecular mechanisms and signaling pathways involved in the development of osteosarcoma, the most aggressive type of bone tumor striking mainly in adolescence and early adulthood, are discussed in detail. The most critical pathways in osteosarcoma pathogenesis are the Notch, Wnt, NF-κB, p53, PI3K/Akt, and MAPK pathways. The balance between cell survival and apoptosis is determined by the Wnt and NF-κB pathways, as well as by the ratio between the activities of the MAPK and PI3K/Akt pathways. Several miRNAs (miR-21, -34a, -143, -148a, -195a, -199a-3p, -382) regulate multiple target genes, pathways, and processes essential for osteosarcoma pathogenesis. Data on the key genes and regulatory miRNAs involved in metastasis and tumor cell response to drug treatment are presented. Possible applications of miRNA in osteosarcoma diagnostics and treatment are discussed.


Assuntos
Neoplasias Ósseas/patologia , MicroRNAs/metabolismo , Osteossarcoma/patologia , Neoplasias Ósseas/genética , Neoplasias Ósseas/metabolismo , Humanos , NF-kappa B/metabolismo , Metástase Neoplásica , Osteossarcoma/genética , Osteossarcoma/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Transdução de Sinais , Proteína Supressora de Tumor p53/metabolismo , Proteínas Wnt/metabolismo
10.
Biochemistry (Mosc) ; 80(2): 145-62, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25756530

RESUMO

Interaction between microRNA (miRNA) and messenger RNA of target genes at the posttranscriptional level provides fine-tuned dynamic regulation of cell signaling pathways. Each miRNA can be involved in regulating hundreds of protein-coding genes, and, conversely, a number of different miRNAs usually target a structural gene. Epigenetic gene inactivation associated with methylation of promoter CpG-islands is common to both protein-coding genes and miRNA genes. Here, data on functions of miRNAs in development of tumor-cell phenotype are reviewed. Genomic organization of promoter CpG-islands of the miRNA genes located in inter- and intragenic areas is discussed. The literature and our own results on frequency of CpG-island methylation in miRNA genes from tumors are summarized, and data regarding a link between such modification and changed activity of miRNA genes and, consequently, protein-coding target genes are presented. Moreover, the impact of miRNA gene methylation on key oncogenetic processes as well as affected signaling pathways is discussed.


Assuntos
Carcinogênese/genética , Metilação de DNA , MicroRNAs/genética , Carcinogênese/metabolismo , Ilhas de CpG , Regulação Neoplásica da Expressão Gênica , Genes , Regiões Promotoras Genéticas , Transdução de Sinais/genética
11.
Arkh Patol ; 76(5): 20-5, 2014.
Artigo em Russo | MEDLINE | ID: mdl-25543404

RESUMO

OBJECTIVE: To make a comparative retrospective clinical and morphological analysis of cases of post-Chernobyl (technogenic) and sporadic (cryptogenic) papillary thyroid carcinoma (PTC) in children and adolescents in the Republic of Belarus. MATERIAL AND METHODS: Nine hundred and thirty-six patients aged less than 19 years, operated on in 1990-2005, when cancer incidence in this age group was directly related to the consequences of the Chernobyl accident (technogenic carcinoma) were examined. A comparison group included their 140 peers who were born after March 1987 and treated in 2005-2010 for PTC (cryptogenic carcinoma). The Kruskal-Wallis test was used to compare quantitative variables; the Fisher, Pearson, and Fisher-Freeman-Halton tests were employed to compare categorical variables. All calculations were made using the R package version 2.15.0. The results were considered to be statistically significant at p<0.05. RESULTS: Comparison of cases of cryptogenic carcinoma and those of technogenic carcinomas diagnosed in 1990-1995 and 1996-2001 revealed substantial differences in the clinical and morphological patterns of the disease. In both mentioned periods, the patients with technogenic PTC were younger than those with cryptogenic PTC (p<0.0001 and p=0.0014, respectively). The proportion of male patients in the technogenic carcinoma group was much higher than that in the cryptogenic carcinoma one (p=0.0006 and p=0.0031). The patients with technogenic carcinoma were also more frequently observed to have infiltrative tumor growth (p=0.0003 and p=0.0169) and lung metastastic involvement (p=0.0001 and p=0.0008). What is more, the architectonics of technogenic versus cryptogenic carcinoma more often contained a solid component (p<0.0001 and p<0.0001), marked intratumoral fibrosis (p=0.0008 and p=0.0266), mononuclear infiltration (p<0.0001 and p<0.0001), and no baseline abnormality (p<0.0001 and p<0.0001). CONCLUSION: In spite of its age similarity, technogenic carcinoma proved to be more clinically aggressive than cryptogenic carcinoma: the extent of organ invasion and the infiltrative growth of carcinoma were more frequently observed.


Assuntos
Carcinoma/patologia , Carcinoma/cirurgia , Neoplasias da Glândula Tireoide/patologia , Neoplasias da Glândula Tireoide/cirurgia , Adolescente , Adulto , Carcinoma/tratamento farmacológico , Carcinoma/embriologia , Carcinoma Papilar , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Estadiamento de Neoplasias , República de Belarus , Estudos Retrospectivos , Câncer Papilífero da Tireoide , Neoplasias da Glândula Tireoide/tratamento farmacológico , Neoplasias da Glândula Tireoide/embriologia
12.
Vopr Onkol ; 60(2): 43-6, 2014.
Artigo em Russo | MEDLINE | ID: mdl-24919260

RESUMO

There is presented clinical and morphological characteristics of post-Chernobyl papillary thyroid cancer in 936 children and adolescents. In general, carcinoma of these patients featured by locally advanced growth - 57.4% (387 of 674 patients with this sign could be assessed), metastases in regional lymph nodes - 73,7% (N1b in 40.7%) and internal organs - 11.1%. The mean duration of follow-up was 12,4 +/- 3,5 years (range 4.3 to 19.6 years) including children 14,6 +/- 2,7 years (range 8.8 to 19.6 years) and adolescents - 10,1 +/- 3,1 years (range 4.3 to 18.8 years). Overall survival for the 20-year period was 96,6% +/- 1,2%. The causes of death were suicide (7), injuries and accidents (5), secondary malignancies (1), somatic diseases (2). Only in two patients the death was related to the main disease - lung metastases. Free-recurrence survival for the cohort of post-Chernobyl carcinomas was 92,7% +/- 1,0%.


Assuntos
Carcinoma/epidemiologia , Carcinoma/patologia , Acidente Nuclear de Chernobyl , Neoplasias da Glândula Tireoide/epidemiologia , Neoplasias da Glândula Tireoide/patologia , Adolescente , Carcinoma/diagnóstico , Carcinoma/mortalidade , Carcinoma Papilar , Causas de Morte , Criança , Pré-Escolar , Intervalo Livre de Doença , Feminino , Seguimentos , Humanos , Neoplasias Pulmonares/secundário , Masculino , República de Belarus/epidemiologia , Câncer Papilífero da Tireoide , Neoplasias da Glândula Tireoide/diagnóstico , Neoplasias da Glândula Tireoide/mortalidade , Adulto Jovem
13.
Vopr Onkol ; 59(2): 121-5, 2013.
Artigo em Russo | MEDLINE | ID: mdl-23814862

RESUMO

Comparison of intact thyroid tissue in children and adolescents operated for papillary thyroid carcinoma, with the reference values established for this age group, found that half of the operated patients had this amount exceeded the upper limit of age and gender norms. In multivariate assessment an exceed of the upper limit of norm was associated with a younger age (under 11 years), living in areas with moderate iodine deficiency, radiogenic history, the presence of Hashimoto thyroiditis, variant of tumor growth and the degree of intratumoral fibrosis.


Assuntos
Carcinoma/cirurgia , Doenças da Glândula Tireoide/etiologia , Doenças da Glândula Tireoide/patologia , Glândula Tireoide/patologia , Neoplasias da Glândula Tireoide/cirurgia , Tireoidectomia , Adolescente , Fatores Etários , Carcinoma/epidemiologia , Carcinoma/patologia , Carcinoma Papilar , Criança , Deficiências Nutricionais/complicações , Deficiências Nutricionais/patologia , Feminino , Fibrose/complicações , Doença de Hashimoto/complicações , Doença de Hashimoto/patologia , Humanos , Iodo/deficiência , Masculino , Análise Multivariada , Federação Russa/epidemiologia , Câncer Papilífero da Tireoide , Glândula Tireoide/anatomia & histologia , Neoplasias da Glândula Tireoide/epidemiologia , Neoplasias da Glândula Tireoide/patologia
14.
Mol Biol (Mosk) ; 46(5): 773-85, 2012.
Artigo em Russo | MEDLINE | ID: mdl-23156677

RESUMO

To date, there are more than two thousand human miRNAs, each of them may be involved in the regulation of hundreds of protein coding target genes. Methylation of CpG-islands, in turn, affects miRNAs gene expression. Our aim was to evaluate the role of methylation in the regulation of miRNA gene expression and, consequently, in the regulation of expression of target genes in primary lung tumors. Using a common collection of non-small cell lung cancer samples we performed a comprehensive study, including analysis of the methylation status and expression levels of some miRNA genes and their potential target genes on chromosome 3: RAR-beta2 and NKIRAS1. Increased frequency of methylation in lung tumors compared to histologically normal tissue was revealed for miR-9-1 and miR-34b/c genes with significant statistics (P < or = 0.05 by Fisher exact test) and for miR-9-3 and miR-193a was marginally significant (P < or = 0.1). Significant correlation was revealed between alterations of methylation and expression level of miR-9-1 gene (P = 5 x 10(-12) by Spearman) in the lung tumors, this suggests the role of methylation in the regulation of expression of this miRNA genes. Besides, a statistically significant negative correlation (P = 3 x 10(-12)-5 x 10(-13) by Spearman) was found between alterations of expression levels of miR-9-1 and miR-17and RAR-beta2 target gene and also between expression level alterations of miR-17 and NKIRAS1 that was not previously analyzed. The inverse relationship between expression levels of miRNA genes and their target genes is consistent with the known mechanism of suppression of protein coding genes expression under the action of miRNAs. For the first time significant correlations (P = 3 x 10(-10)-4 x 10(-13) by Spearman) were shown between alterations of methylation status of miRNA genes (miR-9-1, miR-9-3, miR-34b/c, miR-193a) and the expression level of RAR-beta2 target gene and between alterations of methylation status of miR-34b/c, and miR-193a and the expression level of NKIRAS1 target gene in the primary lung tumors, which suggests the possibility of indirect effects of methylation of miRNA genes on expression level of target genes.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/genética , Proteínas de Transporte/genética , Regulação Neoplásica da Expressão Gênica , Neoplasias Pulmonares/genética , Pulmão/metabolismo , MicroRNAs/genética , Receptores do Ácido Retinoico/genética , Proteínas Repressoras/genética , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Carcinoma Pulmonar de Células não Pequenas/patologia , Proteínas de Transporte/metabolismo , Cromossomos Humanos Par 3 , Ilhas de CpG/genética , Metilação de DNA , Perfilação da Expressão Gênica , Humanos , Pulmão/patologia , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patologia , MicroRNAs/metabolismo , Reação em Cadeia da Polimerase , Receptores do Ácido Retinoico/metabolismo , Proteínas Repressoras/metabolismo
16.
Arkh Patol ; 73(6): 26-9, 2011.
Artigo em Russo | MEDLINE | ID: mdl-22379896

RESUMO

The clinico-morphological investigations of 117 children and adolescences with papillary thyroid carcinoma, surgically treated in the Republic Centre for Thyroid Tumors, Minsk, Belarus in 1995-2009 and dutifully followed-up have been studied. Ninety -five observations of all cases were sporadic, but 22 patients had been treated earlier from other neoplasm (Hodgkin's diseases, malignant lymphoma, leukemia, sarcoma, medulloblastoma). Epidemiologic, clinical and pathological peculiarities of thyroid carcinoma in Belarus were analyzed. A number of features distinguished "cryptogenic" and "iatrogenic" papillary thyroid carcinoma in children and adolescents were found out.


Assuntos
Carcinoma Papilar/patologia , Doença Iatrogênica , Segunda Neoplasia Primária/patologia , Neoplasias da Glândula Tireoide/patologia , Adolescente , Carcinoma Papilar/etiologia , Carcinoma Papilar/cirurgia , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Análise Multivariada , Invasividade Neoplásica , Metástase Neoplásica , Segunda Neoplasia Primária/etiologia , Segunda Neoplasia Primária/cirurgia , Neoplasias da Glândula Tireoide/etiologia , Neoplasias da Glândula Tireoide/cirurgia
19.
Vopr Onkol ; 55(3): 351-7, 2009.
Artigo em Russo | MEDLINE | ID: mdl-19670737

RESUMO

The role of either pre- or intraoperative detection of thyroid cancer for estimating extent of surgery is not important unless malignancy of neoplasm can be established before postoperative stage. Primary morphological diagnosis may be affected by numerous subjective and objective factors. Yet repeat operation can be avoided if puncture biopsy, imprint smears and scarification alongside standard methods of evaluation of frozen sections are used in a complex.


Assuntos
Carcinoma Papilar/patologia , Carcinoma Papilar/cirurgia , Neoplasias da Glândula Tireoide/patologia , Neoplasias da Glândula Tireoide/cirurgia , Adulto , Idoso , Biópsia por Agulha , Diagnóstico Diferencial , Feminino , Secções Congeladas , Humanos , Período Intraoperatório , Modelos Logísticos , Masculino , Pessoa de Meia-Idade
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