Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 7 de 7
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
J Clin Pathol ; 73(1): 30-34, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31315894

RESUMO

AIMS: The purpose of the present study was to elucidate the presence of human herpesvirus 6A (HHV-6A), HHV-6B and HHV-7 in samples of the uterine cervix through detection of viral DNA. We analysed normal tissues, samples with low-grade squamous intraepithelial lesions (LSILs) and high-grade squamous intraepithelial lesions (HSILs). We correlated the presence of HHV-6 and HHV-7 with the finding of human papillomavirus (HPV) in mucosal samples. METHODS: Cervical samples were examined and grouped as follows: group 1 (n=29), normal cytology; group 2 (n=61), samples with LSIL; group 3 (n=35), samples with HSIL. Molecular biology examinations were performed in all samples to detect HHV-6, HHV-7 and HPV DNA and to typify HHV-6 species. RESULTS: Group 1: normal cytology and HPV (-): HHV-6: 6.8% (2/29), HHV-7: 79.3% (23/29); group 2: LSIL and HPV (-): HHV-6: 93.1% (27/29), HHV-7: 96.5% (28/29); LSIL and HPV (+): HHV-6: 0% (0/32), HHV-7: 90.6% (29/32); group 3: HSIL and HPV (-): HHV-6: 20% (2/10), HHV-7: 70% (7/10); HSIL HPV (+): HHV-6: 12% (3/25), HHV-7: 68% (17/25). HHV-6A DNA was not detected in any samples. CONCLUSIONS: (1) Both HHV-6 and HHV-7 infect the mucosal cells of the cervix with higher prevalence of HHV-7. (2) The higher prevalence of HHV-6 in LSIL HPV (-) samples compared with those with normal cytology indicates that it constitutes a possible risk factor for atypia production. (3) The presence of HHV-7 in all samples questions its role in the production of atypia. (4) The finding of HHV-6 and HHV-7 suggests that the cervical mucosa is a possible transmission pathway for these viruses.


Assuntos
Neoplasia Intraepitelial Cervical/diagnóstico , DNA Viral/genética , Herpesvirus Humano 6/genética , Herpesvirus Humano 7/genética , Técnicas de Diagnóstico Molecular , Infecções por Roseolovirus/diagnóstico , Lesões Intraepiteliais Escamosas Cervicais/diagnóstico , Neoplasias do Colo do Útero/diagnóstico , Adolescente , Adulto , Argentina , Neoplasia Intraepitelial Cervical/genética , Neoplasia Intraepitelial Cervical/virologia , Feminino , Testes de DNA para Papilomavírus Humano , Humanos , Pessoa de Meia-Idade , Papillomaviridae/genética , Infecções por Papillomavirus/diagnóstico , Infecções por Papillomavirus/genética , Infecções por Papillomavirus/virologia , Valor Preditivo dos Testes , Prognóstico , Estudos Retrospectivos , Infecções por Roseolovirus/genética , Infecções por Roseolovirus/transmissão , Infecções por Roseolovirus/virologia , Lesões Intraepiteliais Escamosas Cervicais/genética , Lesões Intraepiteliais Escamosas Cervicais/virologia , Neoplasias do Colo do Útero/genética , Neoplasias do Colo do Útero/virologia , Adulto Jovem
2.
Transfus Med Hemother ; 45(6): 423-428, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30800032

RESUMO

Introduction: Since anti-D immunoprophylaxis given to D-negative pregnant women is a blood product, blood donations have an impact on the availability of prophylactic doses. The Pan American Health Organization reported, in June 2017, that less than half of blood donors are volunteers in Latin America and the Caribbean. In these countries, guidelines for use of anti-D prophylaxis are still controversial. The aim of this study was to demonstrate the convenience of a simple and cost-effectivene non-invasive prenatal diagnostic assay for anti-D prophylaxis optimization in multiethnic populations. Methods: Cell-free fetal DNA from plasma samples of D-negative pregnant women were analyzed by real-time PCR for simultaneous amplification of sequences of exons 5 and 10 of the RHD gene. Fetal RHD genotype was determined in 111 pregnant women. Neonates' phenotype was determined 72 h after birth. Results: Genotyping predicted fetal phenotype with 100% accuracy. Prenatal diagnosis showed 78% RHD-positive and 22% RHD-negative neonates. Conclusion: We demonstrated that, beyond the large genetic variation of the Rh system and the numerous D variants present in multiethnic groups, non-invasive fetal RHD genotyping using two sequences of the gene can be enough for clinical application in an admixed population. This robust technique of simple implementation allows to determine fetal RHD in maternal blood with high sensitivity, specificity, and accuracy. The introduction of fetal RhD genotyping as part of an antenatal screening program constitutes a reliable manner to optimize anti-D prophylaxis; however, it has not been implemented so far in most American countries.

3.
Asian Pac J Cancer Prev ; 17(5): 2689-94, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27268652

RESUMO

BACKGROUND: Most studies of human papilloma virus (HPV) are aimed at the natural history of the infection and its relation to cancer; however, there are few studies to assess knowledge of the general population. Our aim was analyze the degree of knowledge of Argentinians about HPV infection and its prevention. MATERIALS AND METHODS: We conducted a voluntary, anonymous and non-binding survey with 27 multiple-choice items, in twelve private and public establishments, selected to include a broad population in terms of education, age and gender. The survey consisted of three sections: individual characteristics of the volunteer, HPV infection basic knowledge, its prevention and the virus relationship with other cancers. RESULTS: One thousand two hundred ninety seven volunteers aged 18 to 80 participated. The total number of correct answers was 45.1%. The correct answers for relationship HPV and cervical cancer was 62.1%. Almost 55% did not know about types of HPV that the vaccines for protection. Statistical analysis showed that women, single people, workers, the better educated, those who have had a STDs or HPV and receiving information through medical or educational establishments had greater knowledge of the topic. Only 0.2% of participants answered all questions correctly. CONCLUSIONS: Knowledge plays an important role in health care and the deficiency found in our population could influence the success of the measures taken in the fight against cervical cancer. In this regard, we believe it would be appropriate, not only to emphasize early diagnosis and vaccine implementation, but also incorporate new communication strategies, facilitating reception of accurate and precise information by all strata of society.


Assuntos
Conhecimentos, Atitudes e Prática em Saúde , Papillomaviridae/patogenicidade , Infecções por Papillomavirus/prevenção & controle , Vacinas contra Papillomavirus/uso terapêutico , Neoplasias do Colo do Útero/prevenção & controle , Adolescente , Adulto , Argentina , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/virologia , Aceitação pelo Paciente de Cuidados de Saúde , Prognóstico , Inquéritos e Questionários , Neoplasias do Colo do Útero/virologia , Vacinação , Adulto Jovem
4.
Córdoba; s.n; 2015. 168 p. ilus.
Tese em Espanhol | LILACS | ID: biblio-831446

RESUMO

Las infecciones zoonóticas son una creciente amenaza para la salud mundial. Las neumonías atípicas son causadas frecuentemente por patógenos zoonóticos como por ejemplo Chlamydia; sin embargo, varias de estas especies bacterianas y sus implicancias son aún poco conocidas. El objetivo del estudio fue profundizar en el conocimiento eco-epidemiológico de las especies de Chlamydia de importancia médico-veterinaria presentes en la provincia de Córdoba, Argentina. Para tal fin, se implementaron técnicas serológicas y moleculares para la detección, cuantificación y caracterización genética de Chlamydia en un amplio rango de muestras humanas [individuos sanos (n=314), individuos con nexo epidemiológico asociado a psitacosis (n=44) y animales [aves silvestres (n=505), aves en cautiverio (n=288), reptiles (n=30), equinos (n=30)]. La especie de Chlamydia más frecuentemente detectada en humanos fue C. pneumoniae, seguida de C. pecorum y C. psittaci. También se detectaron co-infecciones. Este hallazgo no pudo asociarse al sexo, edad, cuadros clínicos específicos, patrón estacional, ni especie aviar de contacto. Sin embargo, la neumonía atípica fue el cuadro clínico más fuertemente asociado al hallazgo de estos agentes y las infecciones mixtas estuvieron asociadas a mayor cuantificación bacteriana y a una exacerbación del cuadro clínico, llevando a la hospitalización de los pacientes, quienes requirieron cuidados intensivos.


SUMMARY: Zoonotic infections are a growing threat to global health. Atypical pneumonias are often caused by zoonotic pathogens such as Chlamydia. However; very little is known about chlamydial infections and their implications in our region The aim of this study was to enhance the eco-epidemiological knowledge of Chlamydia species in the province of Córdoba, Argentina. Serological and molecular techniques were implemented for the detection, quantification and genetic characterization of Chlamydia from a wide range of human samples [healthy individuals (n = 314) and individuals with suspected human psittacosis (n = 44) as well as animal samples [wild birds (n = 505), captive birds (n = 288), reptiles (n = 30), horses (n = 30)]. C. pneumoniae was the most frequently detected species in humans, followed by C. psittaci and C. pecorum. Co-infections were also detected. We did not find associated with sex, age, specific clinical conditions, seasonal pattern, or avian contact. However, atypical pneumonia was the main clinical manifestation associated with these agents. Mixed infections were associated with increased DNA quantification and an exacerbation of clinical symptoms, leading to hospitalization of patients who required intensive care.


Assuntos
Humanos , Masculino , Feminino , Chlamydophila pneumoniae , Chlamydophila psittaci , Evolução Molecular , Especiação Genética , Impactos na Saúde , Estudos Soroepidemiológicos , Argentina/epidemiologia
5.
Diagn Microbiol Infect Dis ; 74(3): 320-2, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22917583

RESUMO

In central area of Argentina, the epidemiologic and molecular characteristics of Chlamydophila psittaci infections are still unknown. Nested polymerase chain reaction of domains II, III, and IV of the omp A gene was used to detect Chlamydophila in 43 pharyngeal swab samples from patients with suspected human psittacosis (2010-2011); 9 (21%) of them yielded positive results. Molecular typing was performed by direct sequencing demonstrating the presence of C. psittaci genotypes A, E/B, and WC.


Assuntos
Chlamydophila psittaci/classificação , Chlamydophila psittaci/genética , DNA Bacteriano/genética , Psitacose/microbiologia , Adolescente , Adulto , Idoso , Animais , Argentina/epidemiologia , Criança , Chlamydophila psittaci/isolamento & purificação , DNA Bacteriano/química , Feminino , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Epidemiologia Molecular , Dados de Sequência Molecular , Tipagem Molecular , Nasofaringe/microbiologia , Reação em Cadeia da Polimerase , Psitacose/epidemiologia , Análise de Sequência de DNA , Adulto Jovem
6.
Rev Inst Med Trop Sao Paulo ; 54(1): 11-6, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22370748

RESUMO

Human papillomavirus (HPV) can induce a wide spectrum of squamous intraepithelial lesions (SIL) of varying severity. The aim of the present study was to establish the frequency of HPV infection and identify the genotypes circulating in women from Córdoba, Argentina, in relation to age and cytology. A total of 186 women, between 18 and 65 years old, with antecedents of SIL, underwent a pelvic examination and had cervical cells collected for cytology and HPV DNA detection. Ninety-six samples (51.6%) were positive for HPV detection, and sixty-three (65.6%) of them showed the presence of at least one HR-HPV. Low- and high-grade SIL showed significant association in patients younger than 35 years of age. We found 18 different genotypes, with a greater presence of HR-HPV. Genotypes 16 and 6 were the most frequent. Seven (7.3%) multiple infections, 85.7% of which had at least one HR-HPV, were detected. The detection of a large number of different HPV genotypes is a warning sign. It is thus necessary to strengthen the monitoring of the circulation of high-risk genotypes, currently less prevalent in intraepithelial lesions, as a control measure for the possible impact of the implementation of vaccines against genotypes 16 and 18.


Assuntos
Neoplasia Intraepitelial Cervical/virologia , Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/virologia , Displasia do Colo do Útero/virologia , Neoplasias do Colo do Útero/virologia , Adolescente , Adulto , Idoso , Argentina/epidemiologia , Neoplasia Intraepitelial Cervical/epidemiologia , DNA Viral/análise , Feminino , Genótipo , Humanos , Pessoa de Meia-Idade , Papillomaviridae/genética , Infecções por Papillomavirus/epidemiologia , Displasia do Colo do Útero/epidemiologia , Neoplasias do Colo do Útero/epidemiologia , Esfregaço Vaginal , Adulto Jovem
7.
Rev. Inst. Med. Trop. Säo Paulo ; 54(1): 11-16, Jan.-Feb. 2012. graf, tab
Artigo em Inglês | LILACS | ID: lil-614890

RESUMO

Human papillomavirus (HPV) can induce a wide spectrum of squamous intraepithelial lesions (SIL) of varying severity. The aim of the present study was to establish the frequency of HPV infection and identify the genotypes circulating in women from Córdoba, Argentina, in relation to age and cytology. A total of 186 women, between 18 and 65 years old, with antecedents of SIL, underwent a pelvic examination and had cervical cells collected for cytology and HPV DNA detection. Ninety-six samples (51.6 percent) were positive for HPV detection, and sixty-three (65.6 percent) of them showed the presence of at least one HR-HPV. Low- and high-grade SIL showed significant association in patients younger than 35 years of age. We found 18 different genotypes, with a greater presence of HR-HPV. Genotypes 16 and 6 were the most frequent. Seven (7.3 percent) multiple infections, 85.7 percent of which had at least one HR-HPV, were detected. The detection of a large number of different HPV genotypes is a warning sign. It is thus necessary to strengthen the monitoring of the circulation of high-risk genotypes, currently less prevalent in intraepithelial lesions, as a control measure for the possible impact of the implementation of vaccines against genotypes 16 and 18.


El papilomavirus humano (human papilloma, HPV) induce un amplio espectro de lesiones intraepiteliales escamosas (SIL) de variada severidad. Objetivo: conocer la frecuencia de infección por HPV y determinar los genotipos circulantes en mujeres de la ciudad de Córdoba, Argentina, en relación con la edad y la citología. Se realizó citología y detección de ADN-HPV en células cervicales de 186 mujeres de 18 a 65 años con antecedentes de SIL. Noventa y seis (51.6 por ciento) fueron positivas para la detección del HPV, de las cuales, en 63 (65.6 por ciento) se detectó la presencia de al menos, un HPV de Alto Riesgo (HR-HPV). Las SIL de alto grado (HSIL) y de bajo grado (LSIL) se asociaron a pacientes menores de 35 años. Se hallaron 18 genotipos diferentes, con mayor presencia de HR-HPV. HPV 16 y 6 fueron más frecuentes y se detectaron 7 (7.3 por ciento) infecciones múltiples, 85.7 por ciento de éstas presentaron al menos un HR-HPV. La detección de un alto número de diferentes genotipos es una señal de alerta. Por tanto, es necesario fortalecer la vigilancia de los HR-HPV, actualmente menos frecuentes en las SIL, como medida de control del impacto que tendrá la implementación de las vacunas contra HPV 16 y 18.


Assuntos
Adolescente , Adulto , Idoso , Feminino , Humanos , Pessoa de Meia-Idade , Adulto Jovem , Neoplasia Intraepitelial Cervical/virologia , Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/virologia , Displasia do Colo do Útero/virologia , Neoplasias do Colo do Útero/virologia , Argentina/epidemiologia , Neoplasia Intraepitelial Cervical/epidemiologia , DNA Viral/análise , Genótipo , Papillomaviridae/genética , Infecções por Papillomavirus/epidemiologia , Displasia do Colo do Útero/epidemiologia , Neoplasias do Colo do Útero/epidemiologia , Esfregaço Vaginal
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA