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1.
Spectrochim Acta A Mol Biomol Spectrosc ; 282: 121646, 2022 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-35926284

RESUMO

Anthraquinone dyes have been widely used to color textile fibers since antiquity. Identification of the dyes can help us understand the dyeing processes and when and where the textiles were produced. Herein, we present a strategy based on surface-enhanced Raman scattering (SERS) with a novel silver colloid substrate for the detection of anthraquinone dyes. Quasi-spherical silver particles with different sizes were prepared by the solvothermal method and then characterized by transmission electron microscopy (TEM). The silver colloid substrates exhibited high-density hot spots with good reproducibility (RSDs of 3 âˆ¼ 16 %) and high sensitivity. Among them, Ag-C2 (the molar ratio of AgNO3 to PVP is 0.367, reacted for 2 h) was used to detect anthraquinone dyes in reference silk fibers as well as ancient textile samples due to the highest sensitivity and the low RSD (5.37 %) in this study. More importantly, Ag-C2 can be utilized to distinguish three madder species (Rubia tinctorum, Rubia cordifolia, and Rubia argyi) depending on the SERS intensity of alizarin and purpurin.

2.
Nat Rev Nephrol ; 2022 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-35788561

RESUMO

Kidney fibrosis, characterized by excessive deposition of extracellular matrix (ECM) that leads to tissue scarring, is the final common outcome of a wide variety of chronic kidney diseases. Rather than being distributed uniformly across the kidney parenchyma, renal fibrotic lesions initiate at certain focal sites in which the fibrogenic niche is formed in a spatially confined fashion. This niche provides a unique tissue microenvironment that is orchestrated by a specialized ECM network consisting of de novo-induced matricellular proteins. Other structural elements of the fibrogenic niche include kidney resident and infiltrated inflammatory cells, extracellular vesicles, soluble factors and metabolites. ECM proteins in the fibrogenic niche recruit soluble factors including WNTs and transforming growth factor-ß from the extracellular milieu, creating a distinctive profibrotic microenvironment. Studies using decellularized ECM scaffolds from fibrotic kidneys show that the fibrogenic niche autonomously promotes fibroblast proliferation, tubular injury, macrophage activation and endothelial cell depletion, pathological features that recapitulate key events in the pathogenesis of chronic kidney disease. The concept of the fibrogenic niche represents a paradigm shift in understanding of the mechanism of kidney fibrosis that could lead to the development of non-invasive biomarkers and novel therapies not only for chronic kidney disease, but also for fibrotic diseases of other organs.

3.
RSC Adv ; 12(26): 16886-16892, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35754890

RESUMO

Geographical origin and authenticity are two core factors to promote the development of traditional Chinese medicine (TCM) herbs perception in terms of quality and price. Therefore, they are important to both sellers and consumers. Herein, we propose an efficient, accurate method for discrimination of genuine and non-authentic producing areas of TCM by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Take Atractylodes macrocephala Koidz (AMK) of compositae as an example, the MALDI-TOF MS spectra data of 120 AMK samples aided by principal component analysis-linear discriminant analysis (PCA-LDA), partial least squares discriminant analysis (PLS-DA) and random forest (RF) successfully differentiated Zhejiang province, Anhui province and Hunan province AMK according to their geographical location of origin. The correct classification rates of test set were above 93.3%. Furthermore, 5 recollected AMK samples were used to verify the performance of the classification models. The outcome of this study can be a good resource in building a database for AMK. The combined utility of MALDI-TOF MS and chemometrics is expected to be expanded and applied to the origin traceability of other TCMs.

4.
Zhongguo Zhong Yao Za Zhi ; 47(11): 2899-2908, 2022 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-35718510

RESUMO

Chinese medicinal materials are the precious resources of China and favored by patients at home and abroad because of their natural sources and curative effects. Pesticides are often used to prevent and control diseases and insect pests and regulate the growth of Chinese medicinal plants, so as to improve the yield and quality of Chinese medicinal materials. Most of the pesticides can play a role in pest control through systemic action, stomach toxicity, contact, fumigation and other ways, especially the systemic pesticides can kill hidden pests by entering the Chinese medicinal plants. Despite the good pest control effect, it is difficult to remove the systemic pesticides by simple cleaning, which poses a great risk to the safety of Chinese medicinal materials. At the same time, excessive or non-standard use of pesticides leads to serious pesticide residues in Chinese medicinal materials, which affects not only the quality and efficacy of the materials and harm human health but also the international development of Chinese medicinal materials industry. Pesticide residues have become a bottleneck affecting the industry development and hindering the export of Chinese medicinal materials. Therefore, it is of great significance to study how to quickly, sensitively, and accurately detect and remove pesticide residues in Chinese medicinal materials. We reviewed the common pesticide residues in Chinese medicinal materials in recent years in terms of characteristics, harm, and detection and removal techniques, and discussed the future development of the detection and removal deve-lopment. With this review, we aimed to provide a reference for the quality control of Chinese medicinal materials and promote the healthy development of Chinese medicine industry.


Assuntos
Resíduos de Praguicidas , Praguicidas , Plantas Medicinais , Fumigação , Humanos , Medicina Tradicional Chinesa , Resíduos de Praguicidas/análise , Praguicidas/análise , Praguicidas/toxicidade
5.
J Org Chem ; 87(12): 7975-7988, 2022 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-35658477

RESUMO

Nucleophilic recyclization of pyridinium salts involving a CCN interchange ring transformation for the synthesis of 2-methylnicotinonitrile derivatives was herein developed. 3-Aminocrotononitrile (3-ACN) produced in situ from CH3CN acted as a C-nucleophile, as well as the source of CH3 and CN groups, which was supported by isotope-labeling and control experiments.

6.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 34(5): 514-518, 2022 May.
Artigo em Chinês | MEDLINE | ID: mdl-35728854

RESUMO

OBJECTIVE: To investigate the correlation between dead space fraction and lung ultrasound score (LUS) and their prognostic value in patients with acute respiratory distress syndrome (ARDS). METHODS: The data of 98 patients with ARDS treated in the intensive care unit (ICU) of the First Affiliated Hospital of Jinzhou Medical University from January to December 2020 were collected and analyzed. The gender, age, acute physiology and chronic health evaluation II (APACHE II), oxygenation index, dead space fraction and LUS score immediately and 24, 48, 72 and 96 hours after ICU admission and 28-day outcomes of all patients were collected. Pearson correlation was used to analyze the correlation between dead space fraction and LUS score. Binary Logistic regression was performed to analyze whether the dead space fraction and LUS score could be the risk factors of the prognosis in patients with ARDS. Receiver operator characteristic curve (ROC) was used to analyze the predictive effect of dead space fraction and LUS score on 28-day mortality in patients with ARDS. RESULTS: A total of 98 patients with ARDS were included, of which 76 cases survived and 22 cases died within 28 days. With the prolongation of ICU stay, the dead space fraction and LUS score in the survival group increased first and then decreased. The dead space fraction and LUS score in the death group continued to increase to 96 hours, and were significantly higher than those in the survival group (dead space fraction: 0.569±0.019 vs. 0.491±0.021, LUS score: 20.09±2.39 vs. 15.13±1.91, both P < 0.05). There was a positive correlation between the dead space fraction and LUS score at 48, 72 and 96 hours in ICU (r values were 0.200, 0.471 and 0.677, all P < 0.05). Binary Logistic regression analysis showed that dead space fraction and LUS score were independent risk factors affecting the prognosis of patients with ARDS [dead space fraction: odds ratio (OR) was 69.064, 95% confidence interval (95%CI) was 22.680-123.499, P = 0.008; LUS score: OR was 4.790, 95%CI was 1.609-14.261, P = 0.005]. The results of ROC curve analysis showed that the dead space fraction at 48, 72 and 96 hours after ICU admission could be used to predict the 28-day mortality of patients with ARDS, the sensitivity was 59.1%, 90.9% and 95.5%, and the specificity was 89.5%, 80.3% and 98.7%. The area under the curve (AUC) of dead space fraction predicting 28-day mortality was 0.802, 0.952 and 0.998, all P < 0.01. The LUS score of 72 hours and 96 hours in ICU could be used to predict the 28-day mortality of patients with ARDS, the sensitivity was 77.3%, 77.3% and 100.0%, and the specificity was 68.4%, 88.2% and 80.3%, respectively. The AUC of the LUS score to predict the 28-day mortality of patients were 0.935 and 0.959, both P < 0.01. CONCLUSIONS: There was significant correlation between dead space fraction and LUS score, both of which were risk factors of 28-day mortality and be used to evaluate the 28-day prognosis of patients with ARDS.


Assuntos
Síndrome do Desconforto Respiratório , APACHE , Humanos , Unidades de Terapia Intensiva , Pulmão/diagnóstico por imagem , Prognóstico , Curva ROC , Síndrome do Desconforto Respiratório/diagnóstico por imagem , Estudos Retrospectivos
7.
Kidney Int ; 2022 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-35644285

RESUMO

Diabetic kidney disease (DKD) is one of the most common and devastating complications of diabetic mellitus, and its prevalence is rising worldwide. Klotho, an anti-aging protein, is kidney protective in DKD. However, its large size, prohibitive cost and structural complexity hamper its potential utility in clinics. Here we report that Klotho-derived peptide 6 (KP6) mimics Klotho function and ameliorates DKD. In either an accelerated model of DKD induced by streptozotocin and advanced oxidation protein products in unilateral nephrectomized mice or db/db mice genetically prone to diabetes, chronic infusion of KP6 reversed established proteinuria, attenuated glomerular hypertrophy, mitigated podocyte damage, and ameliorated glomerulosclerosis and interstitial fibrotic lesions, but did not affect serum phosphorus and calcium levels. KP6 inhibited ß-catenin activation in vivo and blocked the expression of its downstream target genes in glomerular podocytes and tubular epithelial cells. In vitro, KP6 prevented podocyte injury and inhibited ß-catenin activation induced by high glucose without affecting Wnt expression. Co-immunoprecipitation revealed that KP6 bound to Wnt ligands and disrupted the engagement of Wnts with low density lipoprotein receptor-related protein 6, thereby interrupting Wnt/ß-catenin signaling. Mutated KP6 with a scrambled amino acid sequence failed to bind Wnts and did not alleviate DKD in db/db mice. Thus, our studies identified KP6 as a novel Klotho-derived peptide that ameliorated DKD by blocking Wnt/ß-catenin. Hence, our findings also suggest a new therapeutic strategy for the treatment of patients with DKD.

8.
Food Chem ; 394: 133495, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-35753252

RESUMO

Carbaryl is a typical carbamate pesticide that plays an essential role in agricultural production, but its residues cause serious harm to the environment and human health. Here, we developed a polychromatic colorimetric sensor based on ZnTPyP-DTAB peroxidase activity and gold nano-bipyramids (Au NBPs) etching to detect carbaryl. ZnTPyP-DTAB catalyzes the decomposition of H2O2 to hydroxyl radicals, and Au NBPs are etched. The coordination of zinc and nitrogen in nanometer porphyrins was affected by the steric effects of carbaryl, which resulted in decreased activity of ZnTPyP-DTAB peroxidase. The detection limit of carbaryl was 0.26 mg/kg. The recoveries of carbaryl in reaal sample ranged from 91 % to 107% (RSD ≤ 0.7%). The sensor platform displayed a series of high-resolution multicolor variations of rainbow colors within the above concentration range. The rich color variation facilitates the acquisition of digital images. RGB value transformation combined with partial least squares regression model can accurately and quantitatively detect carbaryl in vegetables, fruits and Chinese medicinal materials.


Assuntos
Quimiometria , Ouro , Nanopartículas Metálicas , Carbaril/análise , Quimiometria/métodos , Colorimetria/métodos , Ouro/química , Humanos , Peróxido de Hidrogênio/química , Nanopartículas Metálicas/química , Metaloporfirinas , Peroxidase/química , Porfirinas/química , Compostos de Zinco
9.
Food Chem ; 394: 133473, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-35716498

RESUMO

Identifying geographical origins of red wines made in specific regions is of significance since the false claim of geographical origins has been frequently exposed in China's wine industry. In this work, an untargeted metabolomic approach based on UPLC-QTOF-MS was established to discriminate geographical origins of Chinese red wines. The principal component analysis (PCA) showed significant differences between wine samples from three famous geographical origins in China. The metabolites contributing to the differentiation were screened by orthogonal partial least squares-discriminant analysis (OPLS-DA) with pairwise modeling. 40 and 46 differential metabolites in positive and negative ionization modes were putatively identified as chemical markers. Furthermore, heatmap visualization and OPLS-DA models were constructed based on these identified markers and external verification wine samples from different regions were successfully discriminated, with recognition rate up to 96.7%. This study indicated that UPLC-QTOF-MS-based untargeted metabolomics has great potential for the geographical origin traceability of Chinese red wines.


Assuntos
Vinho , Quimiometria , Cromatografia Líquida de Alta Pressão , Análise Discriminante , Espectrometria de Massas , Metabolômica , Vinho/análise
10.
ACS Appl Mater Interfaces ; 14(26): 29934-29944, 2022 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-35730788

RESUMO

This work presents a low-temperature engineering strategy, from quantum dot (QD) synthesis to fabrication of a hybrid from a homogeneous dispersion to thermal annealing with elaborate use of a small organic molecule dopamine, for achieving a kind of ZnO QD-hybridized piezoelectric polymer film directly integrated into a flexible electrode and a plastic substrate. This strategy is the key for non-transfer assembly of flexible piezoelectric nanogenerators (FPENGs) with both mechanical robustness and high electrical performance via direct lamination. The rational addition of dopamine plays multiple roles of (1) significantly decreasing the size of ZnO particles to a QD level (3.77 nm), (2) formation of a stable and homogeneous dispersion of a ZnO QDs/piezoelectric polyvinylidene fluoride-co-hexafluoropropylene copolymer for uniform hybridization of a piezoelectric film, and (3) increment of the piezoelectric phase via induced crystallization at a low annealing temperature. This dopamine-assisted low-temperature annealing strategy for a hybrid piezoelectric film with a high d33 value (∼31.56 pC/N, 30.56% larger than that of a pure piezoelectric polymer film) required no additional high-voltage polarization treatment and effectively avoided the delamination, distortion, or melt phenomenon between the piezoelectric layer, flexible electrode, and plastic protective layer caused by the high temperature and thermal stress. The obtained FPENGs showed significantly enhanced output performance and mechanical robustness under repeated impact and large amounts of strain conditions. Their specific output voltage and charge density were stably maintained at 7.16 V and 2.40 nC/cm2, which were 30.7 and 50.0% higher than those of FPENGs based on a pure piezoelectric polymer film, respectively. They were further used as biomechanical energy harvesters for generating electricity to charge capacitor energy storage devices for power electronics and self-powered sensors for visual motion-detecting systems, indicating their promising applications in both wearable technology and smart homes.

11.
J Sci Food Agric ; 2022 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-35608930

RESUMO

BACKGROUND: Ascorbic acid (AA) is an essential nutrient for humans, which must be obtained from vegetables, fruits, and other foods. The content of AA has become an important standard to evaluate the quality and nutritional value of food. The fluorescence sensing method based on nanomaterials is a good alternative for the rapid detection of AA. In this study, we developed an inner filter effect-based fluorescent probe that hybridized nitrogen-doped carbon dots (NCDs) with cobalt oxyhydroxide nanoflakes (CoOOH NFs). RESULTS: An optimal NCDs was successfully selected because it has a strong fluorescence at 430 nm and the most significant quenching phenomenon with CoOOH NFs due to the inner filter effect. When adding AA into the NCDs-CoOOH NFs probe solution, a specific redox reaction will occur between the enediol group of AA and the CoOOH NFs to interfere with the quenching ability of CoOOH NFs and recover the fluorescence of NCDs. The recovered fluorescence intensities demonstrated a linear relationship with the concentrations of AA. The assay based on the NCDs-CoOOH NFs probe allows AA to be tested in a wide range of 5-200 µmol L-1 with a detection limit of 2.31 nmol L-1 . Furthermore, to evaluate its practical application, the NCDs-CoOOH NFs fluorescence probe was utilized to analyze AA in vegetable, fruit, and serum matrixes with satisfactory results. CONCLUSION: An inner filter effect-based fluorescence probe for the rapid detection of AA was developed, and it has a good potential to be applied in both food and clinical testing. © 2022 Society of Chemical Industry.

12.
Org Lett ; 24(21): 3878-3883, 2022 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-35609118

RESUMO

Herein, the divergent transformations of 1,3-diols with arylhydrazines via acceptorless dehydrogenative coupling reactions to selectively synthesize pyrazoles and 2-pyrazolines were reported, which were based on Ru3(CO)12/NHC-phosphine-phosphine catalytic systems. The reactions featured low catalyst loading, high selectivity, wide substrate scope, and good yields, with only water and hydrogen gas (H2) as the byproducts.

13.
J Chromatogr A ; 1674: 463121, 2022 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-35605467

RESUMO

In this work, a simple and effective strategy for the determination of 12 active compounds of Atractylodes macrocephala Koidz. (AM) was proposed by using high performance liquid chromatography-diode array detection (HPLC-DAD) combined with alternating trilinear decomposition (ATLD) algorithm. Utilizing the "second-order advantage", three common problems in HPLC could be resolved, namely baseline drifts, peak overlaps, and unknown interferences. 12 compounds were rapidly eluted within 12.5 min, and the average spiked recoveries were 80.8-109.9%. The figures of merit reflected the feasibility of the proposed method. Compared with the results of the traditional univariate calibration method based on HPLC-UV technique, the proposed strategy further verified the reliability and simplicity of the mathematical separation. On this basis, partial least squares-discriminant analysis (PLS-DA) was applied to discriminate 113 AM samples from different geographical origins, and variable importance in projection (VIP) was used to further screen the main differential components that affect the regional division of AM. A series of results show that the AM samples from the three regions have obviously different clustering trends. Overall, the strategy is expected to provide a scientific basis for the modern research of medicinal materials, and it is also conducive to the clinical use and market supervision of AM.


Assuntos
Atractylodes , Calibragem , Cromatografia Líquida de Alta Pressão/métodos , Reprodutibilidade dos Testes
14.
Sci Total Environ ; 838(Pt 2): 156161, 2022 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-35609705

RESUMO

Extracellular electron transfer and pharmaceutical products degradation mechanisms of electrochemical active microbial are helpful in optimizing electricity generation and biotoxic contaminants removal for microbial fuel cells (MFCs). An exoelectrogenic bacterial strain (designated as LYK-6) capable of degrading carbamazepine was first isolated from MFCs operated with carbamazepine as unique fuel. The strain LYK-6 was identified as the member of Pseudomonas genus according to morphological characteristics and 16S rRNA gene sequence analysis. Carbamazepine degradation rate of the strain LYK-6 was determined as 56.28% in inorganic salt medium using carbamazepine as sole carbon source. There were two oxidation peaks located at -0.044 V and 0.288 V revealed with differential pulse voltammetry analysis of the strain LQK-6. The maximum voltage of MFCs inoculated the strain LYK-6 reached to 187 mV when the MFCs fed with carbamazepine. The complete genome of the strain LYK-6 was of 4,454,672 bp in length and encoding 4209 protein genes. Genome annotation and functional gene analysis showed that the strain LYK-6 had significant genes encoding proteins responsible for the degradation of carbamazepine. The results demonstrated that the strain LYK-6 was promising application for the treatment of carbamazepine contaminant water by MFCs. This finding increases the known diversity of exoelectrogens.


Assuntos
Fontes de Energia Bioelétrica , Fontes de Energia Bioelétrica/microbiologia , Carbamazepina , Eletricidade , Eletrodos , Transporte de Elétrons , RNA Ribossômico 16S/genética
15.
Spectrochim Acta A Mol Biomol Spectrosc ; 278: 121273, 2022 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-35537257

RESUMO

An effective method to discriminate Baijiu carries important applications for grade identification and quality control in the Baijiu industry. Herein, we report on a novel and straightforward dual-channel fluorescence sensor array for flavor compounds (FCs) and Chinese Baijiu discrimination. Unit 1 (U1) is the reaction between o-phenylenediamine (OPD) and pyrocatechol (ODHB), and unit 2 (U2) is the reaction between 3,4-diaminotoluene (3,4-DAT) and ODHB. The fluorescent products were changed via FCs in Baijiu relying on the influence of protonation of the amino group on OPD/3,4-DAT and chemical reactions. The array successfully achieves qualitative and quantitative identification of FCs with low detection limits and wide linear ranges. In addition, qualitative identification of 0.7 mmol/L FCs in Baijiu is achieved. Finally, this is applied to discriminate 32 Baijius varieties with different aromas, brands, and grades. The fluorescence sensor array is reliable and straightforward for FCs identification and Baijius discrimination, which is of great significance for authenticity identification in the Baijiu industry.


Assuntos
Catecóis , Odorantes , Fluorescência , Odorantes/análise , Fenilenodiaminas
16.
Spectrochim Acta A Mol Biomol Spectrosc ; 277: 121243, 2022 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-35468376

RESUMO

Eucommia ulmoides is an important and valuable traditional Chinese medicine with various medical functions, and has been widely used as health food in China, Japan, South Korea and other Asian countries for many years. The efficacy and quality of E. ulmoides are closely associated with the geographical origin. In this work, the potential of excitation-emission matrix (EEMs) fluorescence coupled with chemometric methods was investigated for simple, rapid and accurate for identification E. ulmoides from different geographical origins. Parallel factor analysis (PARAFAC) was applied for characterizing the fluorescence fingerprints of E. ulmoides samples. Moreover, k-nearest neighbor (kNN), principal component analysis-linear discriminant analysis (PCA-LDA) and partial least squares discriminant analysis (PLS-DA) models were used for the classification of E. ulmoides samples according to their geographical origins. The results showed that kNN model was more suitable for identification of E. ulmoides samples from different provinces. The kNN model could identify E. ulmoides samples from eight different geographical origins with 100% accuracy on the training and test sets. Therefore, the proposed method was available for conveniently and accurately determining the geographical origin of E. ulmoides, which can expect to be an attractive alternative method for identifying the geographic origin of other traditional Chinese medicines.


Assuntos
Eucommiaceae , Análise Discriminante , Geografia , Análise dos Mínimos Quadrados
17.
Ann Transl Med ; 10(6): 310, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35433965

RESUMO

Background: For patients with multiple organ dysfunction syndrome (MODS), timely assessment of the condition and real-time adjustment of the treatment plan are of critical importance. To this end, transthoracic echocardiography (TTE) is widely used in clinical practice, but whether TTE can improve the short-term prognosis of MODS patients is currently unclear. Methods: We extracted data of patients from the Medical Information Mart for Intensive Care III (MIMIC-III) database and included cases according to inclusion and exclusion criteria. The primary endpoint was the mortality within 28 days after admission to the intensive care unit (ICU), and multi-variate regression analysis was conducted to infer the risk factors associated death within 28 days after ICU admission. Double robust analysis was conducted to investigate the correlation between TTE and the endpoint. A gradient-boosted model (GBM) was constructed to calculate the propensity score (PS) of patients received TTE, so as to reduce the difference of variates between these two groups. Results: A total of 13,844 MODS cases were included and were divided into a TTE group and a non-TTE group. There were 5,022 cases (36.28%) in the TTE group, 2,416 (48.10%) of whom were female; and 8,822 (63.72%) in the non-TTE group, 4,129 of whom (46.80%) were female. The covariates that contributed significantly to PS included chronic heart failure (CHF), creatine kinase (CK), troponin, partial pressure of oxygen (PO2), and sequential organ failure assessment (SOFA) scores. Double robust analysis showed that within 28 days after ICU admission, the TTE group had lower risk of death when compared with the non-TTE group. The adjusted odds ratio (OR) value of TTE for 28 days death was 0.73 [95% confidence interval (CI): 0.65-0.82; P<0.001]. The other 3 models had similar results, suggesting that conduct TTE for patients with MODS in ICU was associated with lower risk of 28 days mortality. Conclusions: TTE can reduce the mortality of MODS patients in the ICU.

18.
Org Lett ; 24(15): 2821-2825, 2022 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-35405076

RESUMO

A direct pyridinium C-H sulfonylimination has been developed for the synthesis of sulfonyl iminopyridine derivatives with high efficiency. This transformation features the direct and efficient formation of a C═N bond with a high functional group tolerance under metal-free conditions. The spectroscopic properties potentially enable these sulfonyl iminopyridine compounds to be useful new emitting materials.

19.
Kidney Int ; 102(1): 96-107, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35341792

RESUMO

Activation of canonical Wnt signaling has been implicated in podocyte injury and proteinuria. As Wnts are secreted proteins, whether Wnts derived from podocytes are obligatory for promoting proteinuria remains unknown. To address this, we generated conditional knockout mice where Wntless, a cargo receptor protein required for Wnt secretion, was specifically deleted in glomerular podocytes. Mice with podocyte-specific ablation of Wntless (Podo-Wntless-/-) were phenotypically normal. However, after inducing kidney damage with Adriamycin for six days, Podo-Wntless-/- mice developed more severe podocyte injury and albuminuria than their control littermates. Surprisingly, ablation of Wntless resulted in upregulation of ß-catenin, accompanied by reduction of nephrin, podocin, podocalyxin, and Wilms tumor 1 proteins. In chronic injury induced by Adriamycin, increased albuminuria, aggravated podocyte lesions and extracellular matrix deposition were evident in Podo-Wntlessl-/- mice, compared to wild type mice. Mechanistically, specific ablation of Wntless in podocytes caused down-regulation of the nuclear factor of activated T cell 1 (NFAT1) and Nemo-like kinase (NLK), key downstream mediators of non-canonical Wnt/calcium signaling. In vitro, knockdown of either NFAT1 or NLK induced ß-catenin activation while overexpression of NLK significantly repressed ß-catenin induction and largely preserved nephrin in glomerular podocytes. Thus, our results indicate that podocyte-derived Wnts play an important role in protecting podocytes from injury by repressing ß-catenin via activating non-canonical Wnt/calcium signaling.


Assuntos
Nefropatias , Podócitos , beta Catenina , Albuminúria/genética , Albuminúria/metabolismo , Albuminúria/prevenção & controle , Animais , Cálcio/metabolismo , Sinalização do Cálcio , Doxorrubicina/toxicidade , Nefropatias/patologia , Camundongos , Podócitos/patologia , Proteinúria/genética , Proteinúria/metabolismo , Proteinúria/prevenção & controle , Via de Sinalização Wnt/fisiologia , beta Catenina/genética , beta Catenina/metabolismo
20.
J Org Chem ; 87(7): 4550-4559, 2022 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-35293759

RESUMO

The acceptorless dehydrogenative cross-coupling of primary alcohols to form cross-esters with the liberation of H2 gas was enabled using a [RuCl(η6-C6H6)(κ2-CNP)][PF6]Cl complex as the catalyst. This sustainable protocol is applicable to a broad range of primary alcohols, particularly for the sterically demanding ones, featuring good functional group tolerance and high selectivity. The good catalytic performance can be attributed to the nitrogen-phosphine-functionalized N-heterocyclic carbene (CNP) ligand, which adopts a facial coordination mode as well as the facile dissociation of coordinated benzene.

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