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1.
Food Chem ; 368: 130651, 2022 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-34392117

RESUMO

Bovine serum albumin (BSA) and BSA-glucose conjugates (GBSAⅠ and GBSAⅠI) with different extent of glycation were complexed with curcumin (CUR). The formation mechanism of BSA/GBSA-CUR complexes and the effect of glycation on their physicochemical properties were investigated. Fluorescence quenching and FTIR analysis indicated that the BSA/GBSA-CUR nanocomplexes were formed mainly by hydrophobic interactions. XRD analysis demonstrated that CUR was present in an amorphous state in the nanocomplexes. BSA with a greater extent of glycation (BSA < GBSAⅠ

Assuntos
Curcumina , Glicosilação , Interações Hidrofóbicas e Hidrofílicas , Soroalbumina Bovina
2.
Diabetes Obes Metab ; 23(9): 2125-2136, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34048142

RESUMO

AIM: To determine the overall efficacy of high- versus low-dose sodium-glucose co-transporter-2 (SGLT2) inhibitors in patients with type 2 diabetes (T2D). MATERIAL AND METHODS: A literature search using MEDLINE, EMBASE and the Cochrane Library was performed from 1 January 2006 to 23 September 2020. Random effects models were used to calculate mean differences (MDs) and pooled relative risk (RR). Prespecified subgroup analyses for each SGLT2 inhibitor, follow-up and controls were performed. Leave-one-out sensitivity and meta-regression analyses were conducted. RESULTS: A total of 51 randomized controlled trials involving 23 989 participants (weighted mean age, 58.9 years; men, 58.8%) were eligible for our meta-analysis. For glycaemic regulation ability, a significant reduction in HbA1c (MD -0.080%, 95% confidence interval [CI] -0.100 to -0.060), fasting plasma glucose (MD -0.227 mmol/L, 95% CI -0.282 to -0.173) and postprandial plasma glucose (MD -0.834 mmol/L, 95% CI -1.268 to -0.400) levels was observed in the high-dose SGLT2 inhibitor group. Treatment with high-dose SGLT2 inhibitors enabled easier achievement of the target (HbA1c <7%) than low-dose SGLT2 inhibitors (RR 1.148, 95% CI 1.104 to 1.193). High-dose SGLT2 inhibitor-based treatment resulted in more efficient regulation of body weight and blood pressure (body weight: MD -0.346 kg, 95% CI -0.437 to -0.254; systolic blood pressure: MD -0.583 mmHg, 95% CI -0.903 to -0.263; diastolic blood pressure: MD -0.352 mmHg, 95% CI -0.563 to -0.142). The results were similar in sensitivity analyses. CONCLUSIONS: The overall efficacy of SGLT2 inhibitors, mainly canagliflozin, dapagliflozin and empagliflozin, was found to be dose dependent.


Assuntos
Diabetes Mellitus Tipo 2 , Inibidores do Transportador 2 de Sódio-Glicose , Simportadores , Glicemia , Diabetes Mellitus Tipo 2/tratamento farmacológico , Humanos , Hipoglicemiantes/uso terapêutico , Masculino , Pessoa de Meia-Idade , Ensaios Clínicos Controlados Aleatórios como Assunto , Sódio , Inibidores do Transportador 2 de Sódio-Glicose/uso terapêutico
3.
Cell Mol Bioeng ; 14(2): 187-199, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33868499

RESUMO

A fast and low-cost fabrication process of flexible hanging drop chips for 3D spheroid cultures was proposed by cutting and bonding Parafilm®, a cohesive thermoplastic. The Parafilm® Hanging Drop Chip (PHDC) was assembled by two-layer of Parafilm® sheet with different sizes of holes. The hole on the upper layer of the Parafilm® is smaller than the hole on the bottom layer. The impact of hole size and sample volume on hanging drop formation and 3D spheroid formations in the hanging drop were investigated. The results showed that 20 µL solution on PHDC with a 3 mm hole could form stabile drop and facilitate spheroid formation. The initial cell number determinates the size of the formed spheroids. Exchanging liquid from the upper hole of the PHDC enables the co-culture of two types of cells in one spheroid and drug efficacy testing in hanging drops. The relative expression of cell adhesion and hypoxia-related genes from spheroids in hanging drop and conventional culture plate suggested the relevance of 3D spheroids and in vivo tumor tissue. The economical hanging drop chip can be fabricated without wet chemistry or expensive fabrication equipment, strengthening its application potential in conventional biological laboratories.

4.
Anal Methods ; 12(33): 4166-4171, 2020 08 27.
Artigo em Inglês | MEDLINE | ID: mdl-32785298

RESUMO

A smartphone-based analysis system is favored for point-of-care testing applications. The present work proposes a novel micro-spectroscopy/imaging system comprising a portable spectrometer as an optical sensor and a compact homemade microscope to acquire the image and spectra of micron-scale regions. Protein concentration quantification based on the bicinchoninic acid method was demonstrated with the proposed micro-spectroscopy/imaging system to analyse the spectrometer signals. Morphologies of onion endothelial and human breast cancer cells, used as biological sample models, were characterized to demonstrate the microscopic imaging capacity of the device. The ability to simultaneously obtain morphological and spectral information using the proposed portable device was demonstrated by examining the 10 µm sub-pixels of a smartphone screen. These results highlight the potential for adopting a smartphone-based micro-spectroscopy/imaging system for point-of-care testing.


Assuntos
Microscopia , Smartphone , Humanos , Testes Imediatos , Refratometria , Análise Espectral
5.
Food Chem ; 328: 127122, 2020 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-32480260

RESUMO

Glycation between target proteins and saccharides is time-consuming or requires high temperatures. Here, a promising reaction medium, natural deep eutectic solvents (NADES), for glucose glycation with bovine serum albumin (BSA) was applied to improve the grafting of glucose-glycated BSA by shifting reaction equilibrium. Two types of glucose-glycated BSA products were prepared using NADES and water systems. SDS-PAGE and MALDI-TOF-MS revealed that BSA and glucose were covalently bonded. Compared with in water system, glycated BSA products in NADES system had more -OH groups, more disordered secondary structures, lower intrinsic fluorescence intensity, and higher ultraviolet-visible absorption. Lower surface hydrophobicity (1100 versus 1356), higher emulsifying activity index (66.17 versus 46.49 m2/g), higher emulsion stability index (79.62 versus 63.61%), and lesser free sulfhydryl (8.07 versus 8.98 µmol/g) groups were obtained with NADES system than with water system. The results suggest that NADES is a suitable alternative reaction medium for promoting the glycation of BSA.


Assuntos
Glucose/química , Soroalbumina Bovina/química , Animais , Glicosilação , Interações Hidrofóbicas e Hidrofílicas , Solventes/química
6.
Mater Sci Eng C Mater Biol Appl ; 111: 110836, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32279765

RESUMO

Solid dispersion is a widely used method to improve the dissolution and oral bioavailability of water-insoluble drugs. However, due to the strong hydrophobicity, the drug crystallization in the release media after drug dissolution and the resulted decreased drug absorption retards the use of solid dispersions. It is widely known that the amphiphilic copolymer can encapsulate the hydrophobic compounds and help form stable nano-dispersions in water. Inspired by this, we tried to formulate the solid dispersion of nimodipine by using amphipathic copolymer as one of the carriers. Concerning the solid dispersions, there are many important points involved in these formulations, such as the miscibility between the drug and the carriers, the storage stability of solid dispersions, the dissolution enhancement and so on. In this study, a systemic method is proposed. In details, the supersaturation test and the glass transition temperature (Tg) measurement to predict the crystallization inhibition, the ratios of different components and the storage stability, the interactions among the components were investigated in detail by nuclear magnetic resonance (1H NMR) and isothermal titration calorimetry (ITC) and, the final dissolution and oral bioavailability enhancement. It was found that the amphiphilic copolymer used in the solid dispersion encouraged the formation the drug loading micelles in the release media and, finally, the problem of drug crystallization in the dissolution process was successfully solved.


Assuntos
Sistemas de Liberação de Medicamentos , Liberação Controlada de Fármacos , Nanopartículas/química , Nimodipina/farmacologia , Tensoativos/química , Administração Oral , Animais , Células CACO-2 , Cristalização , Composição de Medicamentos , Endocitose , Trato Gastrointestinal/efeitos dos fármacos , Humanos , Camundongos , Micelas , Nanopartículas/ultraestrutura , Nimodipina/administração & dosagem , Nimodipina/sangue , Nimodipina/farmacocinética , Polietilenoglicóis/química , Polivinil/química , Povidona/análogos & derivados , Povidona/química , Soluções
7.
J Clin Ultrasound ; 48(1): 19-28, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31609458

RESUMO

OBJECTIVES: To summarize the contrast-enhanced ultrasound (CEUS) features of mediastinal lymphomas and thymic epithelial tumors (including thymomas and thymic carcinomas) and to explore the value of CEUS in the differential diagnosis of lymphomas and thymic epithelial tumors. METHODS: Sixty-nine patients with 69 mediastinal lesions who underwent CEUS and had disease confirmed by histopathology were enrolled in the study. There were 33 cases of lymphoma, 19 cases of thymic carcinoma, and 17 cases of thymoma. CEUS features, including the enhancement pattern, enhancement distribution, enhancement time, inner necrosis status, wash out pattern, and vascular morphology, were evaluated in each group. RESULTS: Thymomas often presented with homogeneous (88.2%, 15/17) and late (88.2%, 15/17) enhancement and a low rate of inner necrosis (17.6%, 3/17). Late (73.7%, 14/19), heterogeneous (68.4%, 13/19), and centripetal (63.2%, 12/19) enhancement were more often observed in thymic carcinoma, as was a high rate of inner necrosis (78.9%, 15/19). Lymphomas showed a homogeneous enhancement rate of 57.6% (19/33) and a late enhancement rate of 54.5% (18/33). The rate of inner necrosis for lymphomas was 45.5% (15/33). The diagnostic accuracy of this finding for distinguishing thymic epithelial tumors from lymphomas was 63.8%, the sensitivity was 80.6%, and the specificity was 45.5%. Enlarged blood vessels were a feature specific to lymphomas, while small vessels arranged in a comb shape was a feature specific to thymic epithelial tumors. CONCLUSION: This study describes the CEUS features of common mediastinal tumors and may stimulate further studies in this field.


Assuntos
Linfoma/diagnóstico por imagem , Neoplasias do Mediastino/diagnóstico por imagem , Neoplasias Epiteliais e Glandulares/diagnóstico por imagem , Timoma/diagnóstico por imagem , Neoplasias do Timo/diagnóstico por imagem , Adulto , Idoso , Meios de Contraste , Diagnóstico Diferencial , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Sensibilidade e Especificidade , Ultrassonografia
8.
Talanta ; 208: 120407, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31816706

RESUMO

To achieve low-cost, compact, and portable nucleic acid testing, an integrated device containing a three-dimensional printing fabricated reverse transcription loop-mediated isothermal amplification (RT-LAMP) chip, a thermal module, and an imaging module was developed. Samples and RT-LAMP reagents were loaded on a sponge-like polyvinyl alcohol pad on a chip, whereas the colorimetric detection zone was a dry paper pre-loaded with Calcine. The sealed chip was incubated on the integrated thermal module, and the RT-LAMP products were pressed into the Calcine pre-loaded dry paper by a stick. Colorimetric changes could be visually observed by the naked eye or imaged with a smartphone camera through the imaging module. For detection of the prostate cancer antigen 3 (PCA3) biomarker, LAMP primers were designed and verified. The specificity of Calcine pre-loaded dry paper based on colorimetric detection of positive LAMP products was investigated. The reaction conditions for on-chip RT-LAMP such as amplification time, temperature, and volume were optimized. Finally, a detection limit of 0.34 fg/µL RNA was achieved with the proposed on-chip RT-LAMP and colorimetric detection method for PCA3. Since the thermal plate is powered by a 12-V battery and the color change can be imaged with a smartphone, the integrated platform can be operated on-site, highlighting its potential in point-of-care testing applications.


Assuntos
Antígenos de Neoplasias/genética , Técnicas de Amplificação de Ácido Nucleico , Neoplasias da Próstata/genética , Biomarcadores/análise , Linhagem Celular Tumoral , Colorimetria , Células Endoteliais da Veia Umbilical Humana , Humanos , Masculino , RNA/análise
9.
Opt Express ; 27(21): 29479-29490, 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31684208

RESUMO

We theoretically investigate the entanglement between two macroscopic rotating mirrors in a Laguerre-Gaussian (L-G) cavity, in which they exchange orbital angular momentum with a same L-G cavity mode, and we examine the influence of various factors such as angular frequencies of two mirrors, effective detuning, temperature and orbital angular momentum of L-G cavity mode on the entanglement. The results show that in some range of mirrors' angular frequencies the entanglement will appear and has strong robustness. And we also study the range of effective detuning and the minimum orbital angular momentum of the L-G cavity mode required to generate entanglement.

10.
Colloids Surf B Biointerfaces ; 174: 426-434, 2019 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-30481703

RESUMO

Emerging evidence has demonstrated that cancer stem cells (CSCs) play critical roles in tumor invasion, metastasis and recurrence. The specific targeting capability on CSCs is of high importance for the development of effective anti-tumor therapeutics. However, isolation, enrichment and cultivation of these special and rare groups of tumor cells for in vitro analyses is a nontrivial job and requires particular culture medium and environmental control. Herein, we established a low-cost and efficient method for CSC enrichment by culturing prostate cancer cells on a hydrophilic filter paper. We found that tumor spheroids could form spontaneously on a pristine filter paper solely with regular cell culture medium. The paper-grown cells had elevated expression of putative CSC markers, indicating increased stemness of the cancer cells. Moreover, increased resistance of the chemotherapeutic drug doxorubicin was observed on the formed CSC spheroids compared to regular culture. The properties of the filter paper were characterized to investigate the underlying mechanism behind the promoted tumor spheroid formation. The obtained results suggested that the excellent hydrophilicity of the cellulose fibers retarded the hydrophobic interaction-mediated cell anchoring on the cellulose fibers, while the limited space/niche between fibers promoted the aggregation of cells. In addition, biocompatible paper-based materials are able to realize convenient assembly of tissue-like structures for developing in vitro disease models or organs-on-paper applications. Therefore, hydrophilic filter papers could be a low-cost material for construction of various assay platforms for isolating and enriching CSCs, screening anti-tumor drugs, and constructing tumor models in vitro.


Assuntos
Proliferação de Células , Células-Tronco Neoplásicas/patologia , Neoplasias da Próstata/patologia , Esferoides Celulares/patologia , Microambiente Tumoral , Humanos , Interações Hidrofóbicas e Hidrofílicas , Masculino , Células Tumorais Cultivadas
11.
Micromachines (Basel) ; 9(12)2018 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-30558236

RESUMO

Cancer cell metastasis has been recognized as one hallmark of malignant tumor progression; thus, measuring the motility of cells, especially tumor cell migration, is important for evaluating the therapeutic effects of anti-tumor drugs. Here, we used a paper-based cell migration platform to separate and isolate cells according to their distinct motility. A multi-layer cells-in-gels-in-paper (CiGiP) stack was assembled. Only a small portion of DU 145 prostate cancer cells seeded in the middle layer could successfully migrate into the top and bottom layers of the stack, showing heterogeneous motility. The cells with distinct migration were isolated for further analysis. Quantitative PCR assay results demonstrated that cells with higher migration potential had increased expression of the ALDH1A1, SRY (sex-determining region Y)-box 2, NANOG, and octamer-binding transcription 4. Increased doxorubicin tolerance was also observed in cells that migrated through the CiGiP layers. In summary, the separation and characterization of prostate cancer cell subtype can be achieved by using the multi-layer CiGiP cell migration platform.

12.
Ying Yong Sheng Tai Xue Bao ; 29(8): 2455-2462, 2018 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-30182582

RESUMO

Fire is one of the major factors that alter structure and function of forest in the Great Xing'an Mountains, with consequences on soil carbon cycling in forests. In this study, we collected soil samples (layers O, A, AB, BC, and C) under different fire-severity levels (low, moderate, and high) and post-fire recovery times (1987-2012) in the forests of Great Xing'an Mountains. Analysis of variance and multiple comparison were used to analyze effects of fire severity and reco-very time on content of soil organic carbon. The results showed that soil organic carbon (SOC) content in layer O presented a rising trend under both moderate- and high-severity fire disturbances. The content of SOC in layers A and B decreased year by year under low- and moderate-severity fires, which ranked in the order: 3 years > 5 years > 10 years > over 10 years since fire. The content of SOC under high-severity fire presented an increasing trend within 10 years since fire distur-bance and then decreased rapidly over 10 years. The content of SOC in layer BC presented no obvious changes.


Assuntos
Carbono , Incêndios , Solo/química , Ciclo do Carbono , China , Florestas
13.
Interv Neuroradiol ; 24(2): 225-228, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28994621

RESUMO

Vertebral artery origin anomalies are typically incidental findings during angiography. We present an extremely rare variant in which the right vertebral artery has a double origin from the right subclavian artery and right common carotid artery in association with an aberrant right subclavian artery, which has never been reported before.


Assuntos
Aneurisma/diagnóstico por imagem , Anormalidades Cardiovasculares/diagnóstico por imagem , Artéria Carótida Primitiva/anormalidades , Doenças Cerebelares/diagnóstico por imagem , Doenças Cerebelares/cirurgia , Hematoma/diagnóstico por imagem , Hematoma/cirurgia , Artéria Subclávia/anormalidades , Artéria Vertebral/anormalidades , Adolescente , Angiografia Digital , Angiografia Cerebral , Feminino , Humanos , Artéria Subclávia/diagnóstico por imagem , Tomografia Computadorizada por Raios X
14.
Zhen Ci Yan Jiu ; 42(5): 423-8, 2017 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-29105471

RESUMO

OBJECTIVE: To observe the effect of manual acupuncture and electroacupuncture (EA) on ultrastructure of facial nerve Schwann cells, myelin sheath and mitochondria in facial nerve injury rabbits, so as to explore its mechanism underlying improving facial palsy. METHODS: A total of 50 New Zealand rabbits were randomly divided into normal, sham-operation, model, MA and EA groups (n=10 in each group). Facial nerve injury model was made by clamping the facial nerve for 5 min using a pair of forceps. Manual needle stimulation (mild reinforcing-reducing) or EA (continuous wave, 20 Hz) was applied to "Dicang" (ST 4), "Xiaguan" (ST 7), "Taiyang" (EX-HN 5) and "Yangbai" (GB 14) on the injured sides for 4 weeks, 30 min each day. The facial nerve motion score was performed every 7 days. The ultrastructure of facial nerve was observed by electron microscope after 28 days' treatment. RESULTS: There were no significant differences in behavioral score and ultrastructure in normal and sham-operation groups (P<0.05). Compared with the normal group, facial nerve motion scores, ultrastructural morphological changes and the number of axons per unit area, myelin sheath thickness and axon area were worse in the model group (P<0.05). After treatment, facial nerve motion scores, ultrastructural morphological changes and the number of axons per unit area, myelin sheath thickness and axon area in the two treatment groups were better than those in the model group (P<0.05), and EA worked better than MA (P<0.05). CONCLUSIONS: In the treatment of facial nerve injury, EA can promote axoplasmic mitochondrial proliferation, myelin sheath recovery and axonal regeneration more effectively than MA, which may be one of the mechanisms that EA therapy is superior to MA.


Assuntos
Terapia por Acupuntura , Eletroacupuntura , Traumatismos do Nervo Facial , Pontos de Acupuntura , Animais , Elétrons , Traumatismos do Nervo Facial/terapia , Coelhos
15.
Cardiovasc Intervent Radiol ; 40(7): 1077-1085, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28271330

RESUMO

PURPOSE: To evaluate the protective effect of using thermo-sensitive basement membrane extract (Matrigel) for hydrodissection to minimize thermal injury to nearby structures and to evaluate its heat sink effect on the ablation zone in radiofrequency ablation (RFA) of the liver. MATERIALS AND METHODS: First, the viscosity profile and heat sink effect of Matrigel were assessed during RFA in vitro and ex vivo. Fresh pig liver tissue was used, and the temperature changes in Matrigel and in 5% dextrose in water (D5W) during RFA were recorded. Then, the size of the ablation zone in the peripheral liver after RFA was measured. Second, in an in vivo study, 45 Sprague-Dawley rats were divided into three groups of 15 rats each (Matrigel, D5W and control). In the experimental groups, artificial ascites with 10 ml of Matrigel or D5W were injected using ultrasound guidance prior to RFA. The frequency of thermal injury to the nearby organs was compared among the three groups, with assessments of several locations: near the diaphragm, the abdominal wall and the gastrointestinal (GI) tract. Finally, the biological degradation of Matrigel by ultrasound was evaluated over 60 days. RESULTS: First, Matrigel produced a greater heat retention (less heat sink) effect than D5W during ex vivo ablation (63 ± 9 vs. 26 ± 6 °C at 1 min on the surface of the liver, P < 0.001). Hepatic ablation zone volume did not differ between the two groups. Second, thermal injury to the nearby structures was found in 14 of 15 cases (93.3%) in the control group, 8 of 15 cases (53.3%) in the D5W group, and 1 of 15 cases (6.7%) in the Matrigel group. Significant differences in the thermal injury rates for nearby structures were detected among the three groups (P < 0.001). The most significant difference in the thermal injury rate was found in locations near the GI tract (P = 0.003). Finally, Matrigel that was injected in vivo was gradually degraded during the following 60 days. CONCLUSIONS: Using thermo-sensitive Matrigel as a hydrodissection material might help reduce the frequency of collateral thermal injury to nearby structures, especially in locations close to the GI tract, compared to conventional D5W. Additionally, Matrigel did not increase the heat sink effect on the ablation zone during ablation and was degraded over time in vivo.


Assuntos
Ablação por Cateter/efeitos adversos , Colágeno , Dissecação/métodos , Laminina , Fígado/cirurgia , Proteoglicanas , Cirurgia Assistida por Computador , Animais , Ascite/cirurgia , Membrana Basal , Queimaduras/prevenção & controle , Combinação de Medicamentos , Temperatura Alta , Técnicas In Vitro , Neoplasias Hepáticas/cirurgia , Masculino , Estudos Prospectivos , Ratos , Ratos Sprague-Dawley , Suínos
16.
Medicine (Baltimore) ; 95(32): e4361, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27512847

RESUMO

The aim of this study was to investigate the role of microflow imaging (MFI) of contrast-enhanced ultrasound (CEUS) for evaluating microvascular architecture of different types of peripheral lung cancer (PLC) and to explore the correlated pathological basis.Ninety-five patients with PLC were enrolled in this study. Two radiologists independently evaluated the microvascular architecture of PLC with MFI. The interobserver agreement was measured with Kappa test. The diagnosis value of MFI was calculated. With pathological analysis, the correlation between MFI and microvascular density (MVD)/microvascular diameter (MD) was evaluated.Of the 95 PLCs, MFI were mainly classified "dead wood" (27.4%, 25.3%), "vascular" (47.4%, 49.5%), and "cotton" (20.0%, 20.0%) patterns by the 2 readers. Kappa test showed a good agreement between the 2 readers (Kappa = 0.758). The "dead wood" can be regarded as a specific diagnostic factor for squamous carcinoma; the sensitivity, specificity, and accuracy was 62.9%, 93.3%, and 82.1%, respectively. The "vascular" and "cotton" patterns correlated well with adenocarcinoma and SCLC (small cell lung cancer); diagnostic sensitivity, specificity, and accuracy were 86.7%, 65.7%, and 78.9%, respectively. MVD of "dead wood" was lower than "vascular" and "cotton," while MD was bigger than the other 2 patterns (P < 0.05). There was a good correlation between MFI and histopathological types of PLC as well as between MFI and MVD/MD (P < 0.05).MFI has the advantage to display the microvascular architecture of PLCs and might become a promising diagnostic method of histopathological types of PLC. MFI features also correlated well with its pathological basis, including MVD and MD.


Assuntos
Neoplasias Pulmonares/patologia , Pulmão/irrigação sanguínea , Neovascularização Patológica/diagnóstico , Hexafluoreto de Enxofre , Ultrassonografia Doppler/métodos , Adulto , Idoso , Estudos de Coortes , Meios de Contraste , Feminino , Humanos , Pulmão/cirurgia , Neoplasias Pulmonares/fisiopatologia , Neoplasias Pulmonares/cirurgia , Masculino , Pessoa de Meia-Idade , Variações Dependentes do Observador , Cuidados Pré-Operatórios , Estudos Retrospectivos , Sensibilidade e Especificidade
17.
J Ultrasound Med ; 35(7): 1523-32, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27246663

RESUMO

OBJECTIVES: The purpose of this study was to explore the diagnostic value of the arrival time difference between lesions and surrounding lung tissue on contrast-enhanced sonography of subpleural pulmonary lesions. METHODS: A total of 110 patients with subpleural pulmonary lesions who underwent both conventional and contrast-enhanced sonography and had a definite diagnosis were enrolled. After contrast agent injection, the arrival times in the lesion, lung, and chest wall were recorded. The arrival time differences between various tissues were also calculated. RESULTS: Statistical analysis showed a significant difference in the lesion arrival time, the arrival time difference between the lesion and lung, and the arrival time difference between the chest wall and lesion (all P < .001) for benign and malignant lesions. Receiver operating characteristic curve analysis revealed that the optimal diagnostic criterion was the arrival time difference between the lesion and lung, and that the best cutoff point was 2.5 seconds (later arrival signified malignancy). This new diagnostic criterion showed superior diagnostic accuracy (97.1%) compared to conventional diagnostic criteria. CONCLUSIONS: The individualized diagnostic method based on an arrival time comparison using contrast-enhanced sonography had high diagnostic accuracy (97.1%) with good feasibility and could provide useful diagnostic information for subpleural pulmonary lesions.


Assuntos
Meios de Contraste/farmacocinética , Aumento da Imagem/métodos , Neoplasias Pulmonares/diagnóstico por imagem , Ultrassonografia/métodos , Adulto , Idoso , Diagnóstico Diferencial , Feminino , Humanos , Pulmão/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Curva ROC , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Tempo
18.
Chemistry ; 22(4): 1334-9, 2016 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-26661529

RESUMO

Presented herein are two luminescent magnesium coordination polymers (Mg-CPs), namely [Mg2 (H2O)2 (2-NDC)4 (1,10-phen)2] (1) and [Mg2 (H2O)(1,4-NDC)2 (1,10-phen)] (2), in which 2-NDCH=2-naphthalenecarboxylic acid, 1,4-NDCH2 =1,4-naphthalene dicarboxylic acid, and 1,10-phen=1,10-phenanthroline. Based on the mixed ligands, the title compounds exhibit linker-based photoluminescence (PL) properties thanks to the unique configuration of the Mg(2+) ions. The two compounds show interesting dual emission on excitation of the different luminophores of the mixed linkers. In particular, the emissions of compound 2 could be tuned from green to yellow simply by varying the excitation energies. Furthermore, 2 could be excited by using a commercial λ=450 nm blue LED chip to generate white-light emission, which allows the fabrication of a white-light-emitting diode (WLED) with 20 lm W(-1) luminous efficacy. This work may provide a new method for designing tunable PL CPs by using the low-cost and abundant magnesium ion.

19.
Appl Environ Microbiol ; 80(20): 6303-15, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25085495

RESUMO

Subtilomycin was detected from the plant endophytic strain Bacillus subtilis BSn5 and was first reported from B. subtilis strain MMA7. In this study, a gene cluster that has been proposed to be related to subtilomycin biosynthesis was isolated from the BSn5 genome and was experimentally validated by gene inactivation and heterologous expression. Comparison of the subtilomycin gene cluster with other verified related lantibiotic gene clusters revealed a particular organization of the genes apnI and apnT downstream of apnAPBC, which may be involved in subtilomycin immunity. Through analysis of expression of the apnI and/or apnT genes in the subtilomycin-sensitive strain CU1065 and inactivation of apnI and apnT in the producer strain BSn5, we showed that the single gene apnI, encoding a putative transmembrane protein, was responsible for subtilomycin immunity. To our knowledge, evidence for lantibiotic immunity that is solely dependent on a transmembrane protein is quite rare. Further bioinformatic analysis revealed the abundant presence of ApnI-like proteins that may be responsible for lantibiotic immunity in Bacillus and Paenibacillus. We cloned the paeI gene, encoding one such ApnI-like protein, into CU1065 and showed that it confers resistance to paenibacillin. However, no cross-resistance was detected between ApnI and PaeI, even though subtilomycin and paenibacillin share similar structures, suggesting that the protection provided by ApnI/ApnI-like proteins involves a specific-sequence recognition mechanism. Peptide release/binding assays indicated that the recombinant B. subtilis expressing apnI interacted with subtilomycin. Thus, ApnI represents a novel model for lantibiotic immunity that appears to be common.


Assuntos
Bacillus subtilis/genética , Proteínas de Bactérias/imunologia , Bacteriocinas/genética , Bacteriocinas/imunologia , Bacteriocinas/farmacologia , Família Multigênica , Bacillus subtilis/efeitos dos fármacos , Bacillus subtilis/imunologia , Bacillus subtilis/metabolismo , Proteínas de Bactérias/genética , Clonagem Molecular , Farmacorresistência Bacteriana/genética , Farmacorresistência Bacteriana/imunologia , Proteínas de Membrana/imunologia , Proteínas de Membrana/metabolismo , Testes de Sensibilidade Microbiana
20.
Immunol Invest ; 43(4): 360-9, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24484319

RESUMO

Dendritic cells (DCs) are the most powerful antigen-presenting cells that have an important role in the immunity and immune tolerance. Transforming growth factor ß (TGF-ß) is a pleiotropic cytokine widely expressing in various tissues and cells, which regulates cellular proliferation, differentiation and apoptosis of several immune cells and is considered to be a key factor in inducing immune tolerance. The effect of TGF-ß on DCs is very complex. In this study, we further investigated the effect of TGF-ß on inducing immune tolerance of DCs. DCs were differentiated from mice bone marrow cells in the absence or presence of TGF-ß. The phenotype as well as function was studied in detail. We found that TGF-ß limited the expression of CD40, CD83, CD86 and MHCII in DCs, increased CD45RB and indoleamine 2, 3-dioxygenase (IDO) expression in DCs, promoted IL-10 and limited IL-12 secretion by DCs. Moreover, TGF-ß increased the endocytosis ability of DCs and limited the ability of DCs in activating T cells. These results suggest that TGF-ß affects the immunity of DCs and enhances their tolerogenicity.


Assuntos
Células Dendríticas/imunologia , Células Dendríticas/metabolismo , Tolerância Imunológica/genética , Fator de Crescimento Transformador beta/metabolismo , Animais , Antígenos de Superfície/genética , Antígenos de Superfície/metabolismo , Células Dendríticas/efeitos dos fármacos , Endocitose/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos dos fármacos , Imunofenotipagem , Interleucina-10/biossíntese , Interleucina-12/biossíntese , Ativação Linfocitária/efeitos dos fármacos , Masculino , Camundongos , Linfócitos T/efeitos dos fármacos , Linfócitos T/imunologia , Fator de Crescimento Transformador beta/farmacologia
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