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1.
J Med Chem ; 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-35005973

RESUMO

Acute lung injury/acute respiratory distress syndrome (ALI/ARDS) is one of the most common complications in COVID-19. Elastase has been recognized as an important target to prevent ALI/ARDS in the patient of COVID-19. Cyclotheonellazole A (CTL-A) is a natural macrocyclic peptide reported to be a potent elastase inhibitor. Herein, we completed the first total synthesis of CTL-A in 24 linear steps. The key reactions include three-component MAC reactions and two late-stage oxidations. We also provided seven CTL-A analogues and elucidated preliminary structure-activity relationships. The in vivo ALI mouse model further suggested that CTL-A alleviated acute lung injury with reductions in lung edema and pathological deterioration, which is better than sivelestat, one approved elastase inhibitor. The activity of CTL-A against elastase, along with its cellular safety and well-established synthetic route, warrants further investigation of CTL-A as a candidate against COVID-19 pathogeneses.

2.
Reprod Domest Anim ; 2022 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-35044003

RESUMO

Maternal-effect genes (MEGs) accumulate in oocytes during oogenesis and mediate the preimplantation embryo developmental program until activation of the zygote genome. Nlrp5 and Tle6 are required for normal preimplantation and embryonic development. However, the precise function of these MEGs in buffalo (Bubalus bubalis) remains to be elucidated. The aim of this study was to characterize Nlrp5 and Tle6 sequences, and analyze their mRNA and protein expression patterns in somatic tissues, oocytes, and preimplantation embryos of buffalo. The coding sequences of each gene were successfully cloned and characterized. Real-time quantitative reverse transcription PCR results revealed an absence of Nlrp5 or Tle6 transcripts in somatic tissues, with the exception of ovary. Expression levels of Nlrp5 and Tle6 in oocytes increased from the germinal vesicle stage to metaphase II stage, and then gradually decreased during morula and blastocyst stages. Protein expression patterns were confirmed by immunofluorescence analysis. This study lays a foundation for further validation of the function of MEGs in buffalo.

3.
Sex Med ; 10(1): 100475, 2022 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-34999483

RESUMO

INTRODUCTION: Measurement of intra-cavernous pressure (ICP) is an internationally recognized method to evaluate erectile function of animals, however, this process is invasive, destructive, and cannot be repeated, leading to a daunting challenge for monitoring the changes in erectile function throughout the whole treatment duration. AIM: To verify whether infrared ray thermography technology based system could be a good substitution of ICP for evaluating rat erectile function. METHODS: A novel thermal image-based method, infrared ray thermography technology (IRT) was employed to monitor erectile function in erectile dysfunction (ED) rats. To detect the sensitivity and specificity of this new technology, 4 ED rat models (Diabetic, nerve-injury, vascular-injury and aged ED models) were established and subjected to both ICP and IRT test. OUTCOMES: Statistical comparisons were done to test the effectiveness of this new way for detecting and dynamically monitoring erectile function. RESULTS: Based on the data curves obtained from ICP and IRT, the IRT showed a similar trend (including peak value, climbing speed) as that of ICP. IRT is considered as a precise way to monitor the real-time changes of erectile function in all ED rat models. The AUC of peak temperature detected by IRT in DMED, aged ED, vascular-injury ED, the nerve-injury ED and total ED rat models were 0.9811,0.9836,0.9893,0.9989 and 0.9882, respectively. Meanwhile, the AUC of temperature climbing rate were 0.6486,0.8357,0.9184,0.8675and 0.8168.Also,it is a non-invasive process of dynamically monitoring erectile function of a same rat at different time points (before and after drug intervention). The data showed that the real-time recovery by tadalafil was obtained by IRT methods even after treatment for only 2 weeks in the diabetic ED (DMED) rat model. CONCLUSION: A novel noninvasive method for monitoring erectile function in rat ED models was established, and can replace or supplement ICP test. Liu S., Zhao Z. Wang Z. et al., Establishing a Thermal Imaging Technology (IRT) Based System for Evaluating Rat Erectile Function. Sex Med 2021;XX:XXXXXX.

4.
Phys Chem Chem Phys ; 24(3): 1885-1895, 2022 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-34990505

RESUMO

Hydrogen bonds (H-bonds) in poly(vinyl alcohol) (PVA) play a crucial role in macroscopic mechanical properties, particularly for stretchability. However, there is still some ambiguity about the quantitative dependence of H-bond interactions on the mechanical performance, mainly attributed to the difficulty in the discrimination of various H-bond types. Herein, small molecular chemicals as plasticizers were incorporated into the PVA matrix to tailor the H-bonding interactions. By altering the PVA molecular weight, plasticizer type and loading, both the stretchability and H-bond content were regulated on a large scale. By a combination of DMA, IR spectroscopy, MD simulation and solid-state 13C-NMR, every sort of H-bond in PVA was assigned, and their relative fractions were ascertained quantitatively. After correlating the elongation ratio with the relative fraction of the different types of H-bonding interaction, it was found that all the pairs of elongation vs. intermolecular H-bond content derived from different series of PVA/plasticizer films could be plotted into a master curve and exhibited good linearity, indicating that intermolecular H-bonds dominate the mechanical stretchability in PVA films. Our efforts contribute towards an in-depth understanding of performance optimization induced by H-bond manipulation from empirical, phenomenological aspects to intrinsic, numerical insights.

5.
Oncogene ; 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-35034094

RESUMO

The role of B cells in the anti-tumor immune response remains controversial. An increase in the number of B cells in the peripheral blood of some tumor patients has been associated with poor immunotherapy efficacy. However, the mechanism leading to the generation of these cells is not well-described. Using a fibrosarcoma model, we show that intraperitoneal administration of a xenogeneic antigen in tumor-bearing mice evokes large increases in antigen-specific serum immunoglobulin formation compared to tumor-naïve mice. An inability of tumor-bearing mice to induce enhanced antibody production after myeloid cell depletion suggests the antibody responses are CD11b+ myeloid cell-dependent. In vitro, CD11b+ myeloid cells promoted B cell proliferation, activation, and survival. High levels of tumor necrosis factor (TNF)-α were produced by CD11b+ cells, and TNF-α blockade inhibited B cell responses. CD11b+ cells appear to be important promoters of B cell responses and targeting B cells may increase the efficacy of immunotherapy in tumor-bearing hosts.

7.
Sci Total Environ ; 807(Pt 1): 150746, 2022 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-34619186

RESUMO

The moisture content of farmland soils is closely related to the farmland soil environment. Although biochar has been widely studied for farmland soil amelioration in tropical and temperate farmland soils, its application in areas of seasonally frozen soil is rare. In this study, field experiments were conducted to explore the effect of biochar on soil temperature and soil liquid moisture content in seasonally frozen soils and its corresponding mechanism. Biochar was applied to the soil at different rates (3 kg·m-2, 6 kg·m-2, 9 kg·m-2, and 12 kg·m-2) in autumn and spring. Daily monitoring data from the 20 cm soil-layer recorded for one year from the biochar application date were analyzed. The approximate entropy was introduced to explore the complex changes in soil temperature and soil liquid moisture content under biochar application in seasonally frozen soils. According to the calculation of approximate entropy, the application of biochar increased the complexity for most treatments. In the case of ignoring the heterogeneity of snowfall and uneven land tillage to the soil, we infer that this change was caused by changes in properties of the biochar due to the freeze-thaw cycle. The treatment under mixed biochar application in spring and autumn of 9 kg·m-2 had the smallest change in water and heat complexity. And the approximate entropy of this treatment is the smallest. Moreover, in the freezing period, the soil liquid moisture content is positively correlated with the biochar amount applied and negatively correlated with the biochar and soil mixing time. In the melting period, the opposite correlations occur. The changes in soil moisture conditions caused by freezing and thawing restrict the affinity of biochar for water. Therefore, the effect of biochar addition on the soil liquid moisture content varies among different freezing and thawing periods.


Assuntos
Solo , Água , Carvão Vegetal , Fazendas , Congelamento
8.
Shock ; 57(1): 118-130, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34905532

RESUMO

OBJECTIVE: Microglial activation is a vital process in the neuroinflammatory response induced by I/R injury. It has been reported that myocyte enhancer factor (MEF)2D expression in activated microglia is associated with microglia-induced inflammatory responses and plays an important role in neuronal survival. This research aimed to investigate the role and mechanism of MEF2D in microglial activation and neuroinflammation in cerebral I/R in vitro and in vivo. METHODS: A cerebral I/R model was established. In vitro, neuronal, or microglial cells were exposed to oxygen-glucose deprivation and reoxygenation to mimic I/R. MEF2D overexpression was induced, and siRNA was administered in vitro and in vivo. Microglial polarization; MEF2D, nuclear transcription factor (NF)-κb, TLR4, and cytokine levels; neuronal injury; mitochondrial function; brain injury and cognitive function were detected in the different groups in vitro and in vivo. RESULTS: We found that oxygen-glucose deprivation increased MEF2D expression in a time-dependent manner in BV2 cells and primary microglia. MEF2D overexpression inhibited microglial activation, the expression of NF-κb and TLR, cytokine levels, and neuronal injury in microglia exposed to oxygen-glucose deprivation and reoxygenation. In the middle cerebral artery occlusion model, microglial activation, the neuroinflammatory response, mitochondrial dysfunction, brain injury, and cognitive function were improved by MEF2D overexpression and aggravated by MEF2D siRNA treatment. CONCLUSION: These results indicate that MEF2D is a necessary molecule for neuroinflammation regulation and neuronal injury in cerebral ischemia.

9.
J Environ Manage ; 301: 113943, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34731955

RESUMO

To explore the effects of different regulation modes on the soil structure and gas transport characteristics in seasonal permafrost regions, freeze-thaw cycles (FTCs) were used as boundary conditions and three typical soils on the Songnen Plain were used: black soil, baijiang soil and meadow soil. Four treatments were established: biochar addition (B1), straw addition (S1), biochar combined with straw addition (B1S1) and an untreated control (CK). The changes in the proportion of soil water-stable aggregates, total soil porosity (TP), soil water characteristic curves (SWRCs), soil dissolved organic carbon (DOC) and soil air permeability (PL) were analyzed. The results showed that biochar and straw influenced the structure of the three soil types. The proportions of large (2-0.5 mm) and medium (0.5-0.25 mm) aggregates increased significantly. The soil aggregate stability indexes of the treated soils were better than those of the CK, and the three-phase ratios of the treated soils were closer to ideal. The different treatments had particularly obvious effects on the black soil; the generalized soil structure index (GSSI) values reached 95.59, 94.36 and 98.74 in the B1, S1 and B1S1 treatments, respectively. An interaction effect was observed between biochar and straw. B1S1 had a stronger effect than the other treatments, and the soil water holding capacity was significantly improved (FC = 0.317 cm3 cm-3). Under the B1S1 treatment, the DOC contents in black soil, baijiang soil and meadow soil were 160.78 mg/kg, 272.828 mg/kg and 271.912 mg/kg, respectively. Moreover, biochar and straw combined effectively reduced PL fluctuations under FTCs and improved the long-term stability of the soil structure. These results can aid in rational straw and biochar use to achieve comprehensive agricultural waste utilization.


Assuntos
Solo , Água , Carbono , Carvão Vegetal
10.
J Ethnopharmacol ; 284: 114791, 2022 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-34737112

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: The dried aboveground part of Geranium Wilfordii Maxim. (G. Wilfordii) is a traditional Chinese herbal medicine named lao-guan-cao. It has long been used for dispelling wind-dampness, unblocking meridians, and stopping diarrhea and dysentery. Previous investigations have revealed that 50% ethanolic extract of G. Wilfordii has anti-inflammatory and anti-proliferation activities on TNF-α induced murine fibrosarcoma L929 cells. Corilagin (COR) is a main compound in G. Wilfordii with the content up to 1.69 mg/g. Pharmacology study showed that COR has anti-inflammatory, anti-tumor, anti-microorganism, anti-oxidant, and hepatoprotective effects. However, there is no any investigation on its anti-proliferation and anti-inflammation effects in rheumatoid arthritis (RA). AIM OF THE STUDY: The present study aimed to evaluate the potential pharmacological mechanisms of anti-proliferation and anti-inflammation effects of COR in RA. MATERIALS AND METHODS: In vitro, MH7A cells model induced by IL-1ß was used. The anti-proliferation activity of COR was assessed by Cell Counting Kit-8 (CCK-8) assay, and the anti-migration and anti-invasion activity of COR was determined by wound healing assay and transwell assay, respectively. Furthermore, apoptosis assay by flow cytometer was used to measure the pro-apoptotic effect of COR. The mRNA expressions of Bax, Bcl-2, IL-6, IL-8, MMP-1, MMP-2, MMP-3, MMP-9, COX-2, and iNOS were measured by qRT-PCR, and related protein were further verified by ELISA kits or Western blot. Moreover, protein levels associated with NF-κB and MAPK signaling pathways of p65, P-p65, IκBα, P-IκBα, ERK1/2, P-ERK1/2, JNK, P-JNK1/2/3, p38, and P-p38 were determined by Western blot. The nuclear translocation of NF-κB-p65 was detected by immunofluorescent staining. In vivo, adjuvant-induced arthritis (AIA) rat model was used, and the body weight, paw swelling, and arthritis score during the entire period were measured. Histopathological analysis of joints of synovial tissues was also determined. The expression of pro-inflammatory cytokines in serum including IL-6, TNF-α, IL-1ß, and IL-17 were measured. RESULTS: The in vitro results showed that COR could dose-dependently inhibit the proliferation, migration, and invasion of IL-1ß-induced MH7A cells, as well as promote its apoptosis. Moreover, it also suppressed the over-expression of Bcl-2, IL-6, IL-8, MMP-1, MMP-2, MMP-3, MMP-9, COX-2, and iNOS while up-regulated the level of Bax. Besides, the ratios of P-p65/p65, P-IκBα/IκBα, P-ERK/ERK, P-JNK/JNK, and P-p38/p38 were decreased, and the nuclear translocation of p65 induced by IL-1ß was blocked by COR. In vivo results indicated that COR significantly reduced the paw swelling and arthritis score in AIA rats, and inhibited synovial tissue hyperplasia and erosion, as well as inflammatory cells infiltration. It also decreased the serum pro-inflammatory cytokines (IL-6, TNF-α, IL-1ß, and IL-17) production. CONCLUSION: These results revealed that COR exerted anti-rheumatoid arthritis effect, and its underlying mechanisms may be related to inhibiting the proliferation, migration, and invasion of synovial fibroblasts, enhancing cell apoptosis, and suppressing inflammatory responses via downregulating NF-κB and MAPK signaling pathways.

11.
Colloids Surf B Biointerfaces ; 209(Pt 2): 112218, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34801930

RESUMO

Regeneration of urethral defects has been difficult in the clinic. To address it, the collagen/ poly (L-lactide-co-caprolactone) (P(LLA-CL)) nanoyarn scaffold delivering adipose-derived stem cells' exosomes (ADSC-exos) was fabricated. The multipotential differentiation potential of ADSCs were confirmed by Adipogenic, osteogenic, and chondrogenic differentiation. The 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide assay shows that 50% concentration of ADSC-exos nanoyarn scaffold dramatically enhanced the cell viability of fibroblasts. The ADSC-exos nanoyarn scaffold for human foreskin fibroblasts (HFFs) and human urethral scar fibroblasts (HSFs) shows good biocompatibility: theproduction of inflammatory factors IL-6 and Col 1A1 was less, indicating that ADSC-exos had the minimal inflammatory effect of cells. Besides, the cells on the ADSC-exos nanoyarn scaffold did not appear to contribute to DNA damage in the same way as the normal cell's growth did. The HFFs seeding on the ADSC-exos nanoyarn scaffold shows a typical morphology of extending outwards. Urethral repair with ADSC-exos nanoyarn scaffold did not lead to either a sign of urethral stricture or scar formation after 4 weeks post-surgery. The deposition of collagen was less and the epithelial cells formed multiple layer epithelium. The treatment of ADSC-exos stimulated epithelization and vascularization. And the transition from an inflammatory state to a regenerative state was promoted. The ADSC-exos-treated group did not promote the over-proliferation of fibroblasts and the expression of Collagen I. Therefore, the ADSC-exos nanoyarn scaffold has evident, positive effects on wound healing and tissue fibrosis inhibition.


Assuntos
Exossomos , Nanofibras , Uretra , Tecido Adiposo , Colágeno , Humanos , Células-Tronco , Engenharia Tecidual , Cicatrização
12.
World J Clin Cases ; 9(32): 9954-9959, 2021 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-34877336

RESUMO

BACKGROUND: Complex aberration in lung is rare, which may increase risk of vascular injury and cause ligation of wrong pulmonary vein or bronchus by mistake during lung surgery, and result in sever complication like pulmonary congestion or atelectasis. CASE SUMMARY: A 44-year-old female was admitted for a ground glass nodule (24 mm in diameter) in her right upper lobe. Video-assisted thoracoscopic (VATS) right upper lobectomy with lymph nodes dissection was performed. During operation, we simultaneously identified extremely rare aberrations of right preeparterial bronchus, right upper lobe vein behind pulmonary artery and right middle lobe vein drained into left atrium in this patient. The patient was well recovered and discharged at the postoperative-day 4. CONCLUSION: Preoperatively, three-dimensional reconstruction can help to identify inconspicuous variation of pulmonary vessels and bronchus effectively. During lung surgery, if anatomic aberration is suspected, careful dissection of vessels and bronchus will help to confirm whether there is an aberration or not.

13.
Nat Metab ; 2021 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-34873338

RESUMO

Ageing-associated functional decline of organs and increased risk for age-related chronic pathologies is driven in part by the accumulation of senescent cells, which develop the senescence-associated secretory phenotype (SASP). Here we show that procyanidin C1 (PCC1), a polyphenolic component of grape seed extract (GSE), increases the healthspan and lifespan of mice through its action on senescent cells. By screening a library of natural products, we find that GSE, and PCC1 as one of its active components, have specific effects on senescent cells. At low concentrations, PCC1 appears to inhibit SASP formation, whereas it selectively kills senescent cells at higher concentrations, possibly by promoting production of reactive oxygen species and mitochondrial dysfunction. In rodent models, PCC1 depletes senescent cells in a treatment-damaged tumour microenvironment and enhances therapeutic efficacy when co-administered with chemotherapy. Intermittent administration of PCC1 to either irradiated, senescent cell-implanted or naturally aged old mice alleviates physical dysfunction and prolongs survival. We identify PCC1 as a natural senotherapeutic agent with in vivo activity and high potential for further development as a clinical intervention to delay, alleviate or prevent age-related pathologies.

14.
Anal Chem ; 2021 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-34878248

RESUMO

Hydroxyl groups are among the major active surface sites over metal oxides. However, their spectroscopic characterizations have been challenging due to limited resolutions, especially on hydroxyl-rich surfaces where strong hydroxyl networks are present. Here, using nanostructured In2O3 as an example, we show significantly enhanced discrimination of the surface hydroxyl groups, owing to the high-resolution 1H NMR spectra performed at a high magnetic field (18.8 T) and a fast magic angle spinning (MAS) of up to 60 kHz. A total of nine kinds of hydroxyl groups were distinguished and their assignments (µ1, µ2, and µ3) were further identified with the assistance of 17O NMR. The spatial distribution of these hydroxyl groups was further explored via two-dimensional (2D) 1H-1H homonuclear correlation experiments with which the complex surface hydroxyl network was unraveled at the atomic level. Moreover, the quantitative analysis of these hydroxyl groups with such high resolution enables further investigations into the physicochemical property and catalytic performance characterizations (in CO2 reduction) of these hydroxyl groups. This work provides insightful understanding on the surface structure/property of the In2O3 nanoparticles and, importantly, may prompt general applications of high-field ultrafast MAS NMR techniques in the study of hydroxyl-rich surfaces on other metal oxide materials.

15.
Adv Healthc Mater ; : e2101496, 2021 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-34878725

RESUMO

The major obstacles of anti-PD therapy in metastatic tumors are limited drug delivery in primary tumors and metastatic foci, and the lack of tumor-infiltrating lymphocytes (TILs). Here, we constructed a novel cellular membrane nanovesicles platform (M/IR NPs) based on homologous targeting and near-infrared (NIR) responsive release strategy to potentiate PD-1/PD-L1 blockade therapy against metastatic tumors. In tumor-bearing mice, biomimetic M/IR NPs targeted to both primary tumors and their lung metastases. Upon laser irradiation, M/IR NPs reduced cancer-associated fibroblasts (CAFs) in tumor microenvironment, thus increasing the penetration of TILs. When shed from homologous tumor cell membranes, positively charged nanoparticles (IR NPs) core could capture released tumor-associated antigens (TAAs), thereby enhancing the antigen-presenting ability of DCs to activate cytotoxic T lymphocytes. When the photothermal conversion temperature under NIR-laser was higher than 42°C, M/IR NPs initiated the rupture of cell membranes and the responsive release of PD-1/PD-L1 inhibitor BMS, which significantly attenuated tumor-associated immunosuppression and synergistically induced T cellular immunity to inhibit the tumor growth and metastasis. Overall, biomimetic M/IR NPs can improve the targeting and therapeutic efficacy of anti-PD therapy in primary tumors and metastases, opening up a new avenue for the diagnosis and treatment of metastatic tumors in the future. This article is protected by copyright. All rights reserved.

16.
Front Psychol ; 12: 683709, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34880801

RESUMO

With the increasing demand from aging population and seasonal blood shortage, recruiting and retaining blood donors has become an urgent issue for the blood collection centers in China. This study aims to understand intention to donate again from a social cognitive perspective among whole blood donors in China through investigating the association between the blood donation fear, perceived rewards, self-efficacy, and intention to return. A cross-sectional survey was conducted in six cities, which are geographically and socioeconomically distinct areas in Jiangsu, China. Respondents completed a self-administrated questionnaire interviewed by two well-trained medical students. A total of 191 blood donors were included in the current study. Descriptive analysis, correlation analysis, and a generalized linear regression model were used to explore the association between demographic characteristics, psychological factors, and intention to donate again. After controlling other covariates, donors with higher fear scores reported lower intention to return (p = 0.008). Association between self-efficacy and intention to return was statistically significant (p < 0.001), whereas the association between intrinsic rewards (p = 0.387), extrinsic rewards (p = 0.939), and intention to return were statistically insignificant. This study found that either intrinsic rewards or extrinsic rewards are not significantly associated with intention to donate again among whole blood donors in China, and fear is negatively associated with intention to donate again. Therefore, purposive strategies could be enacted beyond appeals to rewards and focus on the management of donors' fear.

17.
Molecules ; 26(23)2021 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-34885838

RESUMO

Prunus mume is a traditional ornamental plant, which owed a unique floral scent. However, the diversity of the floral scent in P. mume cultivars with different aroma types was not identified. In this study, the floral scent of eight P. mume cultivars was studied using headspace solid-phase microextraction (HS-SPME) and organic solvent extraction (OSE), combined with gas chromatography-mass spectrometry (GC-MS). In total, 66 headspace volatiles and 74 endogenous extracts were putatively identified, of which phenylpropanoids/benzenoids were the main volatile organic compounds categories. As a result of GC-MS analysis, benzyl acetate (1.55-61.26%), eugenol (0.87-6.03%), benzaldehyde (5.34-46.46%), benzyl alcohol (5.13-57.13%), chavicol (0-5.46%), and cinnamyl alcohol (0-6.49%) were considered to be the main components in most varieties. However, the volatilization rate of these main components was different. Based on the variable importance in projection (VIP) values in the orthogonal partial least-squares discriminate analysis (OPLS-DA), differential components of four aroma types were identified as biomarkers, and 10 volatile and 12 endogenous biomarkers were screened out, respectively. The odor activity value (OAV) revealed that several biomarkers, including (Z)-2-hexen-1-ol, pentyl acetate, (E)-cinnamaldehyde, methyl salicylate, cinnamyl alcohol, and benzoyl cyanide, contributed greatly to the strong-scented, fresh-scented, sweet-scented, and light-scented types of P. mume cultivars. This study provided a theoretical basis for the floral scent evaluation and breeding of P. mume cultivars.

18.
Molecules ; 26(23)2021 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-34885900

RESUMO

Rearrangement reactions are efficient strategies in organic synthesis and contribute enormously to the development of energetic materials. Here, we report on the preparation of a fused energetic structure of 7-nitro-3,5-dihydro-4H-pyrazolo[4,3-d][1,2,3]triazin-4-one (NPTO) based on a novel Hofmann-type rearrangement. The 1,2,3-triazine unit was introduced into the fused bicyclic skeleton from a pyrazole unit for the first time. The new compound of NPTO was fully characterized using multinuclear NMR and IR spectroscopy, elemental analysis as well as X-ray diffraction studies. The thermal behaviors and detonation properties of NPTO were investigated through a differential scanning calorimetry (DSC-TG) approach and EXPLO5 program-based calculations, respectively. The calculation results showed similar detonation performances between NPTO and the energetic materials of DNPP and ANPP, indicating that NPTO has a good application perspective in insensitive explosives and propellants.

20.
Int J Gen Med ; 14: 8797-8803, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34858047

RESUMO

Objective: Earlobe crease (ELC) has been considered as a skin sign of atherosclerosis, and its pathophysiological mechanism is still unclear. Our study aims to test the hypothesis that ELC patients with lower serum levels of the age-suppressing hormone Klotho, which is not only associated with premature aging but also with endothelial dysfunction, may be associated with atherosclerosis. Methods: A total of 135 patients aged 40-68 years underwent coronary angiography. According to the presence or absence of coronary heart disease (CAD) and ELC, they were divided into three groups: CAD group and ELC group (ELC group, n = 45); no ELC group (non-ELC group, n = 45). There was no ELC or CAD in the control group (control group, n = 45). Serum Klotho concentration was obtained by enzyme-linked immunosorbent assay (ELISA). Results: The Klotho level in the ELC group was 365.6 ± 38.1 pg/mL, while the Klotho level in the non-ELC group was 568.8 ± 44.9 pg/mL. It is worth noting that the Klotho level of the ELC group was significantly lower than that of the non-ELC group (P < 0.001). The serum Klotho level of the control group was higher than that of the non-ELC group (593.3±45.3 vs 568.8±44.9 pg/mL, P = 0.702), but the difference was not statistically significant. Multiple logistic regression analysis showed that the Klotho level is a parameter that affects the appearance of ELC. Conclusion: Serum Klotho levels were considerably lower in patients with ELC. We concluded that the perturbations of Klotho in patients might be associated with ELC and with CAD.

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