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1.
Sci Rep ; 13(1): 426, 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36624144

RESUMO

The Early Jurassic angiosperm Nanjinganthus has triggered a heated debate among botanists, partially due to the fact that the enclosed ovules were visible to naked eyes only when the ovary is broken but not visible when the closed ovary is intact. Although traditional technologies cannot confirm the existence of ovules in a closed ovary, newly available Micro-CT can non-destructively reveal internal features of fossil plants. Here, we performed Micro-CT observations on three dimensionally preserved coalified compressions of Nanjinganthus. Our outcomes corroborate the conclusion given by Fu et al., namely, that Nanjinganthus is an Early Jurassic angiosperm.


Assuntos
Magnoliopsida , Ovário , Óvulo Vegetal , Genitália Feminina , Ovário/diagnóstico por imagem , Microtomografia por Raio-X
2.
BMC Urol ; 23(1): 1, 2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36609237

RESUMO

OBJECTIVE: Urethral stenosis caused by pelvic fracture urethral injury (PFUI) is a complex urological disease, especially for the redo cased. However, to find the proximal end of the posterior urethra, and to avoid injury to the rectum and to forecast to remove the inferior pubic margin are two key points for a successful surgery. These steps can be challenging for even the most experienced urologists. This study is to describe a new technique for understanding the three-dimensional (3D) anatomy of the urethra, which will also aid in surgical planning and simplify urethroplasty. MATERIALS AND METHODS: Three patients underwent routine urethroscopy, X ray urethrography and contrast CT urethrography. The 3D images were then reconstructed, and the data were transmitted to a 3D printer. 3D models were printed with polyacrylic acid to simulate the anatomical structure and relationship of urethral stenosis with pubic symphysis and rectum. Various diagnosis methods were compared with the condition in surgery. The patients and trainee questionnaires were performed. RESULTS: Three models of urethral CT were obtained. These models were presented to patients and trainee doctors along with routine urethroscopy, urethrography, and urethral CT. The scores of patients and trainee question forms demonstrated that the 3D printed urethral stenosis model of pelvic fracture has obvious advantages in urethral adjacency and ease of understanding. The 3D printed urethras were easy to show the pubic symphysis and simulate its excision and exposure of urethra. The model could show the precise distance from urethra to rectum to prevent the rectum injury in surgery. CONCLUSIONS: 3D printing technology can be applied to the preoperative evaluation of urethral stenosis caused by PFUI. It can be auxiliary to understand the anatomical structure of the posterior urethra, the direction of urethral displacement, protecting the rectum and the forecasting for pubectomy. It is especially helpful for the accurate preoperative planning of some complex urethral stenosis and redo cases.


Assuntos
Fraturas Ósseas , Ossos Pélvicos , Estreitamento Uretral , Humanos , Uretra/diagnóstico por imagem , Uretra/cirurgia , Uretra/lesões , Estreitamento Uretral/diagnóstico por imagem , Estreitamento Uretral/etiologia , Estreitamento Uretral/cirurgia , Estudos Retrospectivos , Ossos Pélvicos/diagnóstico por imagem , Ossos Pélvicos/cirurgia , Ossos Pélvicos/lesões , Fraturas Ósseas/diagnóstico por imagem , Fraturas Ósseas/cirurgia , Fraturas Ósseas/complicações
3.
JACS Au ; 3(1): 176-184, 2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36711105

RESUMO

Rational design of highly stable and active metal catalysts requires a deep understanding of metal-support interactions at the atomic scale. Here, ultrathin films of FeO and FeO2-x grown on Pt(111) are used as templates for the construction of well-defined metal nanoclusters. Periodic arrays of Cu clusters in the form of monomers and trimers are preferentially located at FCC domains of FeO/Pt(111) surface, while the selective location of Cu clusters at FeO2 domains is observed on FeO2-x /Pt(111) surface. The preferential nucleation and formation of well-ordered Cu clusters are driven by different interactions of Cu with the Fe oxide domains in the sequence of FeO2-FCC > FeO-FCC > FeO-HCP > FeO-TOP, which is further validated by density functional theory calculations. It has been revealed that the p-band center as a reactivity descriptor of surface O atoms determines the interaction between metal adatoms and Fe oxides. The modulated metal-oxide interaction provides guidance for the rational design of supported single-atom and nanocluster catalysts.

4.
J Clin Med ; 12(1)2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36615138

RESUMO

(1) Background: The study aimed to construct nomograms to improve the detection rates of prostate cancer (PCa) and clinically significant prostate cancer (CSPCa) in the Asian population. (2) Methods: This multicenter prospective study included a group of 293 patients from three hospitals. Univariable and multivariable logistic regression analysis was performed to identify potential risk factors and construct nomograms. Discrimination, calibration, and clinical utility were used to assess the performance of the nomogram. The web-based dynamic nomograms were subsequently built based on multivariable logistic analysis. (3) Results: A total of 293 patients were included in our study with 201 negative and 92 positive results in PCa. Four independent predictive factors (age, prostate health index (PHI), prostate volume, and prostate imaging reporting and data system score (PI-RADS)) for PCa were included, and four factors (age, PHI, PI-RADS, and Log PSA Density) for CSPCa were included. The area under the ROC curve (AUC) for PCa was 0.902 in the training cohort and 0.869 in the validation cohort. The AUC for CSPCa was 0.896 in the training cohort and 0.890 in the validation cohort. (4) Conclusions: The combined diagnosis of PHI and PI-RADS can avoid more unnecessary biopsies and improve the detection rate of PCa and CSPCa. The nomogram with the combination of age, PHI, PV, and PI-RADS could improve the detection of PCa, and the nomogram with the combination of age, PHI, PI-RADS, and Log PSAD could improve the detection of CSPCa.

5.
Artigo em Inglês | MEDLINE | ID: mdl-36627117

RESUMO

BACKGROUND: There is a well-established relationship between high allostatic load (AL) and increased risk of mortality. This study expands on the literature by combined latent profile analysis (LPA) with survival data analysis techniques to assess the degree to which AL status is associated with time to death. METHODS: LPA was employed to identify underlying classes of biological dysregulation among a sample of 815 participants from the Midlife in the US study. Sex-stratified Cox proportional hazards regression models were used to estimate the association between class of biological dysregulation and time to death while controlling for sociodemographic covariates. RESULTS: The LPA resulted in three classes: low dysregulation, immunometabolic dysregulation and parasympathetic reactivity. Women in the immunometabolic dysregulation group had more than three times the risk of death as compared with women in the low dysregulation group (HR=3.25, 95% CI: 1.47 to 7.07), but that there was not a statistically significant difference between the parasympathetic reactivity group and the low dysregulation group (HR=1.80, 95% CI: 0.62 to 5.23). For men, the risk of death for those in the immunometabolic dysregulation (HR=1.79, 95% CI: 0.88 to 3.65) and parasympathetic reactivity (HR=0.90, 95% CI: 0.34 to 3.65) groups did not differ from the low dysregulation group. CONCLUSION: The findings are consistent with the previous research that demonstrates increased AL as a risk factor for mortality. Specifically, in women, that increased risk may be associated with immunometabolic dysregulation and not simply a generalised measure of cumulative risk as is typically employed in AL research.

6.
Artigo em Inglês | MEDLINE | ID: mdl-36630032

RESUMO

As the shallow mineral resources are nearly depleted, the mining of deep resources has become an urgent problem to be studied. The increase in mine depth can lead to the increase of mine heat hazard, which is a critical concern for mining safety/occupational health and safety. However, there are limited review articles available regarding the prevention of mine heat hazard. To fill in this gap, a bibliometric analysis and knowledge mapping of the field of mine heat hazard prevention are presented in this paper. A total of 314 papers from the Web of Science (WOS) core collection database that published between January 1998 and July 2022 were analyzed using VOSviewer and CiteSpace. China, South Africa, Poland, USA, and Australia are the top five countries in this field. The important journals are Applied Thermal Engineering, Applied Energy, Energies, and International Journal of Mining Science and Technology. In addition, the research focal points and two research fronts were identified and discussed. The knowledge base of mine heat hazard research focuses on mine cooling technology, energy efficiency optimization of cooling systems, thermodynamic theory, and occupational health. There are two research fronts. One is to use the numerical simulation method to study various problems such as simulate the performance of refrigeration systems and thermal comfort in mines. The second is to study the occupational health impact of climate change on miners. Therefore, this paper provides readers and academics with an overview of the intellectual structure and knowledge body that have been developed on the subject of mine heat hazard.

7.
BMC Genomics ; 24(1): 8, 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36624393

RESUMO

BACKGROUND: Exosomes are nanosized membranous vesicles secreted by various types of cells, which facilitate intercellular communication by transporting bioactive compounds. Exosomes are abundant in biological fluids including semen, and their protein composition and the potential of seminal plasma exosomes (SPEs) as fertility biomarkers were elucidated in humans, however, little information is available regarding buffalo (Bubalus bubalis). Here, we examined protein correlation between spermatozoa, seminal plasma (SP), and SPEs, and we compared and analyzed protein differences between high-motility (H-motility) and low-motility (L-motility) SPEs in buffalo. RESULTS: SPEs were concentrated and purified by ultracentrifugation combined with sucrose density gradient centrifugation, followed by verification using western blotting, nanoparticle tracking analysis, and transmission electron microscopy. Protein composition in spermatozoa, SP and SPEs, and protein difference in H- and L-motility SPEs were identified by LC-MS/MS proteomic analysis and were functionally analyzed through comprehensive bioinformatics. Many SPEs proteins originated from spermatozoa and SP, and nearly one third were also present in spermatozoa and SP. A series of proteins associated with reproductive processes including sperm capacitation, spermatid differentiation, fertilization, sperm-egg recognition, membrane fusion, and acrosome reaction were integrated in a functional network. Comparative proteomic analyses showed 119 down-regulated and 41 up-regulated proteins in L-motility SPEs, compared with H-motility SPEs. Gene Ontology (GO) enrichment of differentially expressed proteins (DEPs) showed that most differential proteins were located in sperm and vesicles, with activities of hydrolase and metalloproteinase, and were involved in sperm-egg recognition, fertilization, single fertilization, and sperm-zona pellucida binding processes, etc. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that differential proteins were mainly involved in the PPRP signaling pathway, calcium signaling pathway, and cAMP signaling pathway, among others. Furthermore, 6 proteins associated with reproduction were validated by parallel reaction monitoring analysis. CONCLUSION: This study provides a comprehensive description of the seminal plasma exosome proteome and may be of use for further screening of biomarkers associated with male infertility.


Assuntos
Exossomos , Sêmen , Animais , Masculino , Humanos , Sêmen/metabolismo , Búfalos , Motilidade Espermática , Cromatografia Líquida , Exossomos/metabolismo , Proteômica , Espectrometria de Massas em Tandem , Espermatozoides/metabolismo , Proteoma/metabolismo
8.
Chem Rev ; 2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36633446

RESUMO

In recent years, the simple synthesis of artificial humic substances (A-HS) by alkaline hydrothermal processing of waste biomass was described. This A-HS was shown to support water and mineral binding, to change soil structure, to avoid fertilizer mineralization, and to support plant growth. Many of the observed macroscopic effects could, however, not be directly related to the minute amounts of A-HS which have been added, and an A-HS stimulated microbiome was found to be the key for understanding. In this review, we describe such anthropogenic soil in the language of the modern concept of living engineered materials and identify natural and artificial HS as the enabler to set up the interactive microbial system along the interfaces of the mineral grains. In that, old chemical concepts as surface activity, redox mediation, and pH buffering are the base of the system structure build-up and the complex self-adaptability of biological systems. The resulting chemical/biological hybrid system has the potential to address world problems as soil fertility, nutrition of a growing world population, and climate change.

9.
Pharmaceutics ; 15(1)2023 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-36678848

RESUMO

Amorphization is widely used as an effective method of increasing the solubility of insoluble drugs. However, some amorphous drugs exhibit a much lower dissolution rate than their corresponding crystalline form due to their gelation. In this study, we reported the gels formed from amorphous acemetacin (ACM) for the first time. Gelation was promoted at conditions of lower pH, higher temperature and lower ionic strength. Solid-state characterizations suggested that ACM gels may be formed by recrystallization. This mechanism provides a new direction in facilitating the elimination of gelation for amorphous drugs. Moreover, it also provides the basis for the development of sustained-release formulations using the gelation properties.

10.
ACS Synth Biol ; 2023 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-36701126

RESUMO

l-Methionine is the only sulfur-containing amino acid among the essential amino acids, and it is mainly produced by the chemical method in industry so far. The fermentation production of l-methionine by genetically engineered strains is an attractive alternative. Due to the complex metabolic mechanism and multilevel regulation of the synthesis pathway in the organism, the fermentation production of l-methionine by genetically engineered strains was still not satisfied. In this study, the biosynthesis pathway of l-methionine was regulated based on the previous studies. As the competitive pathway and an essential amino acid for cell growth, the biosynthesis pathway of l-lysine was first repaired by complementation of the lysA gene in situ on the genome and then replaced the in situ promoter with the dynamically regulated promoter PfliA to construct a nonauxotroph strain. In addition, the central metabolic pathway and l-cysteine catabolism pathway were further modified to promote the cell growth and enhance the l-methionine production. Finally, the l-methionine fermentation yield in a 5 L bioreactor reached 17.74 g/L without adding exogenous amino acids. These strategies can effectively balance the contradiction between cell growth and l-methionine production and alleviate the complexity of fermentation operation and the cost with auxotroph strains, which provide a reference for the industrial production of l-methionine by microbial fermentation.

11.
World J Urol ; 2023 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-36592177

RESUMO

PURPOSE: The purpose of this study is to identify patients in the prostate imaging reporting and data system (PI-RADS) 3 population who need biopsy by using prostate health index (PHI) and other clinical parameters in order to avoid unnecessary biopsies. METHODS: A total of 302 patients from four hospital were enrolled, and 92 patients with PI-RADS 3 were included finally. All patients were biopsy-naïve and had suspicion of prostate cancer (PCa) with PSA level in 4-20 ng/ml and a normal digital rectal exam. Univariable and stepwise forward multivariable logistic regression analyses were used to evaluated the risk factors. The sensitivity, specificity, and positive and negative predictive values of different cut-off value of PHI were calculated for the diagnosis of clinically significant prostate cancer (CSPCa). RESULTS: The overall patient's mean age was 65.65 ± 9.55 years, median PSA was 7.68 (5.28-12.07) ng/ml and median PHI was 43.80 (33.09-64.69). PCa was identified in 32.61% (30/92) of PI-RADS 3 and CSPCa was identified in 28.26% (26/92) of PI-RADS 3. The risk factors for detecting PCa and CSPCa in multivariable regression analysis were age and PHI. When the biopsy was restricted to those PHI ≥ 43.5, 42.39% unnecessary biopsied could avoid. The sensitivity, specificity, positive predictive value and negative predictive value for the detection of CSPCa in the PHI ≥ 43.5 were 92.31%, 63.64%, 50% and 95.45% respectively. CONCLUSION: The inclusion of PHI in the diagnosis of the PI-RADS 3 population may avoid many unnecessary biopsies. The multivariable models could increase the detection of cancer.

12.
Nanoscale ; 15(4): 1937-1946, 2023 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-36625215

RESUMO

pH-Responsive nanotherapeutics were recently developed for the treatment of ulcerative colitis (UC). However, they target the entire colon rather than the UC site, which leads to insufficient accumulation in inflamed colon lesions and causes side effects. Core-shell nanoparticles exhibit unique advantages in improving the precision of targeted delivery. In this study, Eudragit® EPO and L100, two pH-sensitive materials, were coated on nano-sized curcumin to fabricate core-shell nanoparticles. The developed CNs@EPO@L100 exhibited programmed pH-responsive drug release behavior, improved in vitro anti-inflammatory ability, and enhanced accumulation at the site of inflammation in the colon. Furthermore, after oral administration, CNs@EPO@L100 significantly ameliorated the inflammatory symptoms in mice. Taken together, this study provides insights into programmed release through the rational application of pH-sensitive materials and offers strategies for a precisely targeted therapy of UC using core-shell nanoparticles.


Assuntos
Colite Ulcerativa , Nanopartículas , Camundongos , Animais , Colite Ulcerativa/tratamento farmacológico , Inflamação/tratamento farmacológico , Sistemas de Liberação de Medicamentos , Nanopartículas/uso terapêutico , Concentração de Íons de Hidrogênio
13.
Fish Shellfish Immunol ; 133: 108555, 2023 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-36669604

RESUMO

Sebastes schlegelii is a kind of fish with great economic values. Recently, with the continuous expansion of aquaculture scale and the continuous improvement of aquaculture density, outbreak of various diseases has caused huge economic losses to its aquaculture industry. Study of fish immune system can help to understand the mechanism of immune response to external pathogens and can promote the development of immune prevention and control methods. Immunoglobulins (Igs) are complex glycoproteins that appear to be unique to the vertebrates that can recognize a wide variety of pathogens and recruit immune cells and molecules to destroy pathogens, which are generated by a series of rearrangement and somatic mutations. We therefore studied the immunoglobulin genes of S. schlegelii in view of their important roles in resisting to external pathogen infections. In this study, the immunoglobulin heavy chain genes (sIgM, mIgM, sIgT, and mIgT) of S. schlegelii were successfully identified and cloned. Phylogenetic analysis showed that the IgM and IgT genes of S. schlegelii were clustered together with homologous genes of other species, indicating that they were highly conserved during the evolutionary process. Collinearity analysis showed that the immunoglobulin genes and their adjacent genes were aligned with zebrafish, Atlantic salmon and tilapia, which further confirmed the conserved immunoglobulin gene of teleost. Expression analysis of healthy tissues showed that the expression levels of sIgM, sIgT and mIgT were the highest in the skin, while mIgM was the highest in spleen. After different bacterial infection, IgM and IgT were significantly expressed in skin and gill, which may be because skin and gill are the first line of defense against the infection pathogens. Subcellular localization showed that the mIgT protein was expressed in both the cell membrane and cytoplasm. Meanwhile, recombinant protein of mIgT was obtained in vitro, which laid a foundation for subsequent protein function studies. These results provide a theoretical basis for understanding the immunity role of immunoglobulin in S. schlegelii.

14.
3 Biotech ; 13(2): 65, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36718409

RESUMO

Toxic polycyclic aromatic hydrocarbons (PAHs) are often released into the environment during the combustion and processing of fossil fuels and are capable of causing significant pollution to people and the environment. One of the representative substances of PAHs is phenanthrene, which is often studied as a model compound for PAHs treatment. In this study, we compared the results of transcriptome analysis of Pseudomonas stutzeri LH-42 in two different culture conditions under phenanthrene-induced culture (test group) and glucose-induced culture (control group), and analysed the key enzymatic mechanisms of Pseudomonas stutzeri LH-42 in the biodegradation of phenanthrene. In our experiments, the transcriptome results showed that a total of 380 genes were more than twofold differentially expressed in the test group, of which 187 genes were significantly up-regulated in expression under Phenanthrene induction. Among the 380 differentially expressed genes, 90 genes were involved in Phenanthrene biodegradation, mainly including genes involved in biometabolism, cellular chemotaxis, substrate transport, signal induction and other related processes. Based on the transcriptome sequence analysis of Pseudomonas stutzeri LH-42 at the time of phenanthrene induction, a total of 25 dioxygenase genes were identified, and the related genes were mainly concentrated in two relatively concentrated clusters of PAHs biodegradation genes. The transcriptome analysis resulted in a complete set of enzyme genes related to the phenanthrene biodegradation pathway. The analysis of key enzymes led to the inference of a possible phenanthrene biodegradation pathway: the salicylic acid degradation pathway. The results of this study provide a theoretical basis for in situ remediation of PAHs-contaminated environments using Pseudomonas stutzeri LH-42. Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-023-03473-7.

15.
J Alzheimers Dis ; 2023 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-36641681

RESUMO

BACKGROUND: Previous cross-sectional studies have identified a possible link between Helicobacter pylori (H. pylori) infection and dementia. However, the association of H. pylori infection with longitudinal cognitive decline has rarely been investigated. OBJECTIVE: This cohort study aims to demonstrate the effects of H. pylori infection on longitudinal cognitive decline. METHODS: This cohort study recruited 268 subjects with memory complaints. Among these subjects, 72 had a history of H. pylori infection, and the rest 196 subjects had no H. pylori infection. These subjects were followed up for 24 months and received cognitive assessment in fixed intervals of 12 months. RESULTS: At baseline, H. pylori infected, and uninfected participants had no difference in MMSE scores. At 2 years of follow-up, H. pylori infected participants had lower MMSE scores than uninfected participants. H. pylori infection was associated with an increased risk of longitudinal cognitive decline, as defined by a decrease of MMSE of 3 points or more during follow-up, adjusting for age, sex, education, APOEɛ4 genotype, hypertension, diabetes, hyperlipidemia, and smoking history (HR: 2.701; 95% CI: 1.392 to 5.242). H. pylori infection was associated with larger cognitive decline during follow-up, adjusting for the above covariates (standardized coefficient: 0.282, p < 0.001). Furthermore, H. pylori infected subjects had significantly higher speed of cognitive decline than uninfected subjects during follow-up, adjusting for the above covariates. CONCLUSION: H. pylori infection increases the risk of longitudinal cognitive decline in older subjects with memory complaints. This study is helpful for further understanding the association between infection and dementia.

16.
Appl Opt ; 62(2): 398-410, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36630240

RESUMO

To effectively evaluate the detection capability of the infrared search and tracking system (IRST), the mathematical models of target infrared (IR) radiation intensity envelope, atmospheric transmittance, and signal-to-noise ratio (SNR) operating range are improved, respectively. Based on the above three models, the IRST performance evaluation simulation system is designed to analyze the optimal detection point and effective detection area under different conditions (such as target speed, detection angle, detection probability, false alarm probability, and operating range). Meanwhile, in order to verify the validity of the model, the range evaluation method of calibration and cross-validation is proposed. And taking the cross-validation method as the baseline, the error of the optimized mathematical model of IRST in this paper is within 10%. The research results are of reference significance for IRST operating range evaluation, design, and use.


Assuntos
Modelos Teóricos , Razão Sinal-Ruído , Probabilidade
17.
Sci Total Environ ; : 161593, 2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36642275

RESUMO

As a passivation material for heavy metals in-situ remediation, biochar (BC) has often been expected to maintain long-term adsorption performance for target pollutants. There is still lack of consensus about the impact of aging processes on biochar properties, particularly with respect to its long-term sorption performance. In this study, the changes to immobilization mechanisms as well as the speciation distribution of Cd(II) triggered by combined aging simulation (dry-wet, freeze-thaw cycle and oxidation treatment) on BC prepared under three levels of pyrolysis temperatures (300, 500 and 700 °C) were investigated. The results showed significant inhibition of aging on adsorption performance with the adsorptive capacity of BC300, BC500 and BC700 for Cd(II) decreased by 31.12 %, 50.63 % and 14.94 %, respectively. However, sequential extraction results indicated little influence of the aging process on the relative fractionation of Cd(II) speciation. The distribution of readily bioavailable, potentially bioavailable and non-bioavailable fractions of Cd(II) on BC showed only minimal changes post-aging. Overall, there was less Cd(II) sorption following aging, but the fractional availability (in relative terms) remained the same. Compared with 300 and 700 °C, the biochar prepared under 500 °C accounted the highest fraction of non-bioavailable Cd(II) (67.23 % of BC500, 59.17 % of Aged-500), and thus showed most promising for Cd(II) immobilization. This study has important practical significance for the long-term application of biochar in real environment.

18.
Biomacromolecules ; 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36642871

RESUMO

At-home tooth whitening solutions with good efficacy and biosafety are highly desirable to meet the ever-growing demand for aesthetic dentistry. As a promising alternative to the classic peroxide bleaching that may damage tooth enamel and gums, piezocatalysis has been recently proposed to realize non-destructive whitening by toothbrushing with piezoelectrical particles. However, traditional particles either pose potential threats to human health or exhibit low piezoresponse to weak mechanical stimuli in the toothbrushing. Here, biocompatible and biodegradable polylactide particles constructed from interlocking crystalline lamellae have been hierarchically designed as next-generation whitening materials with ultra-high piezocatalytic activity and biosafety. By simultaneously controlling the chain conformation within lamellae and the porosity of such unique lamellae network at the nano- and microscales, the particles possessing unprecedented piezoelectricity have been successfully prepared due to the markedly increased dipole alignment, mechanical deformability, and specific surface area. The piezoelectric output can reach as high as 18.8 V, nearly 50 times higher than that of common solid polylactide particles. Consequently, their piezocatalytic effect can be readily activated by a toothbrush to rapidly clean the teeth stained with black tea and coffee, without causing detectable enamel damage. Furthermore, these particles have no cytotoxicity. This work presents a paradigm for achieving high piezoelectric activity in polylactide, which enables its practical application in tooth whitening.

19.
BMC Med ; 21(1): 6, 2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36600276

RESUMO

BACKGROUND: Immune checkpoint inhibitor (ICI) therapy combined with conventional therapies is being broadly applied in non-small cell lung cancer (NSCLC) patients. However, the risk of interstitial pneumonitis (IP) following a combined regimen is incompletely characterized. METHODS: A total of 46,127 NSCLC patients were extracted for disproportionality analyses of IP from the Food and Drug Administration's Adverse Event Reporting System (FAERS) database. A total of 1108 NSCLC patients who received ICI treatment at Nanfang Hospital of Southern Medical University were collected and utilized for real-world validation. RESULTS: Of the 46,127 patients with NSCLC, 3830 cases (8.3%; 95% confidence interval [CI], 8.05-8.56) developed IP. Multivariable logistic regression analyses revealed that the adjusted ROR of ICI combined with radiation (RT) was the highest (121.69; 95% CI, 83.60-184.96; P < 0.0001) among all therapies, while that of ICI combined with chemotherapy (CHEMO) or targeted therapy (TARGET) was 0.90 (95% CI, 0.78-1.04; P = 0.160) and 1.49 (95% CI, 0.95-2.23; P = 0.065), respectively, using ICI monotherapy as reference. Furthermore, analyses from our validation cohort of 1108 cases showed that the adjusted odds ratio of ICI combined with RT was the highest (12.25; 95% CI, 3.34-50.22; P < 0.01) among all the therapies, while that of ICI combined with CHEMO or TARGET was 2.32 (95% CI, 0.89-7.92; P = 0.12) and 0.66 (95% CI, 0.03-4.55; P = 0.71), respectively, using ICI monotherapy as reference. CONCLUSIONS: Compared with ICI monotherapy, ICI combined with RT, rather than with CHEMO or TARGET, is associated with a higher risk of IP in NSCLC patients. Hence, patients receiving these treatments should be carefully monitored for IP.


Assuntos
Carcinoma Pulmonar de Células não Pequenas , Doenças Pulmonares Intersticiais , Neoplasias Pulmonares , Humanos , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Neoplasias Pulmonares/tratamento farmacológico , Farmacovigilância , Imunoterapia , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Doenças Pulmonares Intersticiais/epidemiologia , Doenças Pulmonares Intersticiais/tratamento farmacológico , Doenças Pulmonares Intersticiais/etiologia , Estudos Retrospectivos
20.
Funct Integr Genomics ; 23(1): 29, 2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36604355

RESUMO

ThE present work focused on exploring Girdin expression within gastric cancer (GC), examining the effect of Girdin on the cell phenotype of GC, and clarifying the underlying mechanisms. Girdin expression in GC samples was identified by immunohistochemistry (IHC) and quantitative real-time PCR (qRT-PCR) assays. Girdin-targeting siRNAs were transfected into GC cells; later, we examined GC cell proliferation, migration, invasion, and apoptosis, respectively. Additionally, the protein expression was examined through Western blotting assay. Moreover, the tumor implantation experiment was conducted for examining Girdin knockdown in vivo. The results showed that Girdin expression elevated within GC samples, which was associated with the dismal prognostic outcome. Girdin knockdown suppressed GC cell proliferation, migration, and invasion, and enhanced apoptosis and cell cycle arrest. Girdin promoted the phosphorylation of AKT, GSK3ß, and ß-catenin. Moreover, Girdin inhibited the phosphorylation of ß-catenin. Girdin suppressed cell apoptosis and stimulated cell migration and invasion, while AKT inhibitor (MK2206) treatment reversed the effect of Girdin overexpression, and GSK3ß inhibitor (CHIR99021) treatment enhanced the effect of Girdin overexpression on GC cells. Besides, Girdin delayed tumor growth in vivo. In conclusion, Girdin was abnormally expressed in GC samples, which promoted the development of GC by regulating AKT/GSK3ß/ß-catenin signaling.


Assuntos
Proteínas dos Microfilamentos , Proteínas Proto-Oncogênicas c-akt , Neoplasias Gástricas , Proteínas de Transporte Vesicular , Humanos , beta Catenina/genética , beta Catenina/metabolismo , Linhagem Celular Tumoral , Proliferação de Células/genética , Regulação Neoplásica da Expressão Gênica , Glicogênio Sintase Quinase 3 beta/genética , Glicogênio Sintase Quinase 3 beta/metabolismo , Oncogenes , Proteínas Proto-Oncogênicas c-akt/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , Neoplasias Gástricas/metabolismo , Proteínas de Transporte Vesicular/genética , Proteínas de Transporte Vesicular/metabolismo , Proteínas dos Microfilamentos/genética , Proteínas dos Microfilamentos/metabolismo
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