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1.
Toxicology ; 465: 153011, 2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-34715266

RESUMO

Endocrine-disrupting chemicals (EDCs) might increase the risk of childhood diseases by disrupting hormone-mediated processes that are critical for growth and development during childhood, however, the association among the exposure level of EDCs such as Nonylphenol (NP), Bisphenol A (BPA), Dimethyl phthalate (DMP) in children and environmental risk factors, as well as hepatic function has not been elaborated. This study aimed to discuss this interesting relationship among NP, BPA, DMP concentrations in serum, environmental risk factors, hepatic function of 5- to 14-year-old children in industrial zone, residential zone and suburb in northern district of Guizhou Province, China. In Zunyi city, 1006 children participated in cross-sectional health assessments from July to August 2018, and their parents completed identical questionnaires on the environmental risk factors of EDCs exposure to mothers and children. Serum NP, BPA and DMP concentrations were measured by high performance liquid chromatography (HPLC). Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), AST/ALT, total bilirubin (TBIL), direct bilirubin (DBIL) and indirect bilirubin (IBIL) were detected with automatic biochemical analyzer. The median concentrations of serum NP, BPA, and DMP in the participants were 45.85 ng/mL, 26.31 ng/mL and 31.62 ng/mL, respectively, which were higher than the environmental concentration limits of the U.S. National Environmental Protection Agency (EPA). Hair gels used during pregnancy, types of domestic drinking water, nail polish and cosmetics used by children were significantly positive correlated with serum NP concentration (P < 0.05). Gender, feeding pattern, plastic water cup used during pregnancy, hair spray and perfume use for children, duration of children birth, materials for baby bottle or cup and ways to plastic products were significantly positively correlated with serum BPA concentration (P < 0.05). Gender, perms used during pregnancy, hair spray and perfume use for children, using plastic lunch box during pregnancy, duration of children birth, exposure to pesticides, parents' occupations were significantly positively correlated with serum DMP concentrations (P < 0.05). Serum NP (ß = 0.296, P = 0.036) and DMP (ß = 0.316, P = 0.026) concentrations and TBIL level were significantly positively correlated. Serum NP concentration and the levels of IBIL (ß = 0.382, P = 0.006) are significantly positively correlated. Cosmetics used during pregnancy significantly increased AST level (ß = 2.641, P = 0.021). There was a positive correlation between the frequency of hair spray and perfume use for children and the AST (ß = 4.241, P = 0.022). NP, BPA and DMP, which were commonly detected in the serum of children aged 5-14 years old in Zunyi City, Northern Guizhou Province, China, were closely related to the environmental risk factors of exposure environment during pregnancy, infancy and school age. Exposure to NP, BPA and DMP would have negative effects on hepatic function, and these effects showed differences in gender and geographical location. Notably,The relationships were more evident in girls than in boys.

2.
Biopharm Drug Dispos ; 42(9): 427-434, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34651308

RESUMO

To explore the clinical application of a population pharmacokinetics (PPK) model of vancomycin in patients with hematological diseases and neutropenia. Patients with hematological diseases and neutropenia were included in the PPK model study. Nonlinear mixed effect modeling approach (NONMEM) was used for model establishment. Monte Carlo simulation was carried out. A total of 74 patients were divided into model group and non-model group for clinical application research. The model group was given the initial dose of 1g q8h, and the non-model group was given 1g q12h as an empiric initial dosage. The follow-up dose adjustments were made according to the concentration results. This two-compartment model showed good stability and accuracy. The first trough concentration (C0 ) and the compliance rate of the first C0 were much higher in the model group than that in the non-model group (14.30 ± 4.73 µg/ml and 59.38% vs. 8.02 ± 2.61 µg/ml, 35.71%). Less patients needed dose adjustments and fewer adjustment times in the model group than those in the non-model group (12.50% and 0.13 ± 0.34 times vs. 50.00% and 0.61 ± 0.66 times). This suggested that for those patients who had a Creatinine clearance rate (CLCR) ≥ 90 ml/min/1.73 m2 , the initial dose of 1g q8h may help to reach the target C0 (10∼20 µg/ml) quickly. It also helped to reduce the times and number of patients who need dose adjustments. Our PPK model of vancomycin in patients with hematologic diseases and neutropenia can be used to shorten the time to reach the target concentration and reduce the number of dose adjustments.Clinical trial registration: Not applicable (Retrospective study).

3.
World J Clin Cases ; 9(24): 7237-7244, 2021 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-34540984

RESUMO

BACKGROUND: Multiple primary cancer refers to more than one synchronous or sequential cancer in the same individual. Multiple primary cancer always presents as solid cancer or acute myeloid leukemia (AML) secondary to lymphoma. Here, we report a rare case of secondary peripheral T-cell lymphoma and AML after Burkitt lymphoma treatment. CASE SUMMARY: A 54-year-old female patient was admitted to our hospital complaining of edema on her left lower limb. Physical examination revealed multiple superficial lymphadenectasis on her neck and pelvis. Color ultrasonography examination showed multiple uterine fibroids and a solid mass at the lower left side of the abdomen. Pathological biopsy revealed Burkitt lymphoma. After three hyper-CVAD (A + B) regimens, she achieved complete remission. Two years later, lymphadenectasis reoccurred. A relevant biopsy confirmed the diagnosis of peripheral T-cell lymphoma, which was accompanied by gastrointestinal invasion and hemocytopenia. Meanwhile, bone marrow examination revealed AML. On the second day of scheduled treatment, she developed gastrointestinal bleeding, peptic ulcers, and hemorrhagic shock and was critically ill. She was then discharged from the hospital due to financial concerns. CONCLUSION: This is the first report of secondary peripheral T-cell lymphoma and AML after Burkitt lymphoma treatment with heterochronous and synchronal multiple primary cancers.

4.
Front Mol Biosci ; 8: 738219, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34552961

RESUMO

Background: MicroRNA (abbreviated miRNA)-based treatment holds great promise for application as clinical antitumor therapy, but good carriers for delivery of the miRNA drug are lacking. Exosomes secreted by mesenchymal stem cells (MSCs) have proved to be safe, and exogenously modified exosomes may potentially represent an excellent drug delivery vehicle. Methods: In this study, we designed a delivery nano system using single-stranded variable fragment (scFv)-modified exosomes derived from human cord blood MSCs. Genetic engineering technology was used to obtain anti-Glypican 3 (GPC3) scFv-modified exosomes, which were then loaded with miR-26a mimics through electroporation. Results: Results of electron microscopy and dynamic light scattering indicated that the diameter of the drug-carrying exosomes was about 160 nm. Furthermore, anti-GPC3 scFv-modified exosomes effectively delivered miR-26a to GPC3-positive hepatocellular carcinoma cells, thereby inhibiting cell proliferation and migration by regulating the expression of downstream target genes of miR-26a. The exosomes-based nano system displayed favorable anti-tumor effect in vivo with no obvious side effects. Conclusion: Our data provided a new perspective for the use of exosome delivery systems for miRNA-based antitumor therapy.

5.
PeerJ ; 9: e11968, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34447636

RESUMO

Background: Acute myeloid leukemia (AML) is one of the most common blood cancers, and is characterized by impaired hematopoietic function and bone marrow (BM) failure. Under normal circumstances, autophagy may suppress tumorigenesis, however under the stressful conditions of late stage tumor growth autophagy actually protects tumor cells, so inhibiting autophagy in these cases also inhibits tumor growth and promotes tumor cell death. Methods: AML gene expression profile data and corresponding clinical data were obtained from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases, from which prognostic-related genes were screened to construct a risk score model through LASSO and univariate and multivariate Cox analyses. Then the model was verified in the TCGA cohort and GEO cohorts. In addition, we also analyzed the relationship between autophagy genes and immune infiltrating cells and therapeutic drugs. Results: We built a model containing 10 autophagy-related genes to predict the survival of AML patients by dividing them into high- or low-risk subgroups. The high-risk subgroup was prone to a poorer prognosis in both the training TCGA-LAML cohort and the validation GSE37642 cohort. Univariate and multivariate Cox analysis revealed that the risk score of the autophagy model can be used as an independent prognostic factor. The high-risk subgroup had not only higher fractions of CD4 naïve T cell, NK cell activated, and resting mast cells but also higher expression of immune checkpoint genes CTLA4 and CD274. Last, we screened drug sensitivity between high- and low-risk subgroups. Conclusion: The risk score model based on 10 autophagy-related genes can serve as an effective prognostic predictor for AML patients and may guide for patient stratification for immunotherapies and drugs.

6.
Mol Genet Genomic Med ; 8(9): e1369, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32638549

RESUMO

BACKGROUND: Despite targeted sequencing have identified several mutations for leukemia, there is still a limit of mutation screening for Chinese leukemia. Here, we used targeted next-generation sequencing for testing the mutation patterns of Chinese leukemia patients. METHODS: We performed targeted sequencing of 504 tumor-related genes in 109 leukemia samples to identify single-nucleotide variants (SNVs) and insertions and deletions (INDELs). Pathogenic variants were assessed based on the American College of Medical Genetics and Genomics (ACMG) guidelines. The functional impact of pathogenic genes was explored through gene ontology (GO), pathway analysis, and protein-protein interaction network in silico. RESULTS: We identified a total of 4,655 SNVs and 614 INDELs in 419 genes, in which PDE4DIP, NOTCH2, FANCA, BCR, and ROS1 emerged as the highly mutated genes. Of note, we were the first to demonstrate an association of PDE4DIP mutation and leukemia. Based on ACMG guidelines, 39 pathogenic and likely pathogenic mutations in 27 genes were found. GO annotation showed that the biological process including gland development, leukocyte differentiation, respiratory system development, myeloid leukocyte differentiation, mesenchymal to epithelial transition, and so on were involved. CONCLUSION: Our study provided a map of gene mutations in Chinese patients with leukemia and gave insights into the molecular pathogenesis of leukemia.


Assuntos
Loci Gênicos , Mutação INDEL , Leucemia/genética , Polimorfismo de Nucleotídeo Único , Proteínas Adaptadoras de Transdução de Sinal/genética , Adulto , Idoso , Proteínas do Citoesqueleto/genética , Proteína do Grupo de Complementação A da Anemia de Fanconi/genética , Feminino , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Masculino , Pessoa de Meia-Idade , Proteínas Tirosina Quinases/genética , Proteínas Proto-Oncogênicas/genética , Proteínas Proto-Oncogênicas c-bcr/genética , Receptor Notch2/genética , Análise de Sequência de DNA
7.
Environ Res ; 187: 109464, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32438096

RESUMO

BACKGROUND: Except for known cardiovascular risk factors, long-term exposure to environmental endocrine disruptors (EEDs) - a class of exogenous chemicals, or a mixture of chemicals, that can interfere with any aspect of hormone action - has been shown to increase the risk of cardiovascular diseases (CVDs), which are still controversial. OBJECTIVE: To conduct a comprehensive systematic review and meta-analysis to estimate the association between EEDs, including nonylphenol (NP), bisphenol A (BPA), polychlorinated biphenyl (PCB), organo-chlorine pesticide (OCP) and phthalate (PAE) exposure and CVD risk. METHODS: The heterogeneity between different studies was qualitatively and quantitatively evaluated using Q test and I2 statistical magnitude, respectively. Subgroup analysis was performed using chemical homologs - a previously unused grouping method - to extract data and perform meta-analysis to assess their exposure to CVD. RESULTS: Twenty-nine literatures were enrolled with a total sample size of 88891. The results indicated that exposure to PCB138 and PCB153 were the risk factors for CVD morbidity (odds ratio (OR) = 1.35, 95% confidence interval (CI): 1.10-1.66; OR = 1.35, 95% CI: 1.13-1.62). Exposure to organo-chlorine pesticide (OCP) (OR = 1.12, 95% CI: 1.00-1.24), as well as with phthalate (PAE) (OR = 1.11, 95% CI: 1.06-1.17) and BPA (OR = 1.19, 95% CI: 1.03-1.37) were positively associated with CVD risk, respectively. BPA exposure concentration had no correlation with total cholesterol (TC), or low-density lipoprotein (LDL), but exhibited a correlation with gender, waist circumference (WC), high-density lipoprotein (HDL), age, and body mass index (BMI) (standardized mean difference (SMD)) = 1.51; 95% CI: =(1.01-2.25); SMD = 0.16; 95% CI: (0.08-0.23); SMD = -0.19; 95% CI: (-0.27-0.12); SMD = -0.78; 95% CI: (-1.42-0.14); SMD = 0.08; 95% CI: (0.00-0.16). CONCLUSIONS: EED exposure is a risk factor for CVD. Long-term exposure to EEDs can influence cardiovascular health in humans. A possible synergistic effect may exist between the homologs. The mechanism of which needs to be further explored and demonstrated by additional prospective cohort studies, results of in vitro and in vivo analyses, as well as indices affecting CVD.


Assuntos
Doenças Cardiovasculares , Disruptores Endócrinos , Índice de Massa Corporal , Doenças Cardiovasculares/induzido quimicamente , Doenças Cardiovasculares/epidemiologia , Disruptores Endócrinos/toxicidade , Humanos , Estudos Prospectivos , Fatores de Risco
8.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 28(1): 130-135, 2020 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-32027265

RESUMO

OBJECTIVE: To study the expression level of TGFß1 and VEGF gene in patients with acute myeloid leukemia (AML) and its clinical prognostic value. METHODS: Seventy-eight AML patients treated in our hospital from July 2016 to September 2018 were selected. After isolation of bone marrow mononuclear cells from the patients, the levels of TGFß1 and VEGF genes were detected by RT-PCR, and the correlation of TGFß1 with VEGF genes and clinical characteristics of AML patients was analyzed. OS and EFS of the patients were evaluated by Kaplan-Meier, and Cox risk ratio model was used to analyze the prognostic risk factors of AML patients. RESULTS: The relative expression level of TGFß1 gene in AML patients was 0.32±0.04, which was significantly lower than that in control group (P<005). The relative expression level of vascular endothelial growth factor(VEGF) gene in the patients was 2.65±0.15, which was significantly higher than that in the control group (P<0.05). The levels of TGFß1 and VEGF genes significantly correlated with leukocyte count, hemoglobin, platelet and peripheral blast levels in AML patients (P<0.05). The level of TGFß1 in AML patients with complete remission was higher than that in patients with partial remission or non-remission (P<0.05). The level of TGFß1 in AML patients with partial remission was significantly higher than that in patients with non-remission (P<0.05). The level of VEGF in AML patients with complete remission was lower than at in patients with partial remission or non-remission (P<0.05). The level of VEGF in AML patients with partial remission was significantly lower than that in patients with non-remission (P<0.05). Kaplan-Meier survival analysis showed that OS and DFS in AML patients with high expression of TGFß1 were better than those in patients with low expression of TGFß1 (P<0.05), OS and DFS in AML patients with low expression of VEGF were better than those in patients with high expression of VEGF (P<0.05). Multivariate Cox regression analysis showed that platelet, TGFß1 and VEGF gene were independent influencing factors of OS (P<0.05). Leukocyte, TGFß1 and VEGF gene were independent influencing factors of DFS (P<0.05). CONCLUSION: Decreased expression of TGFß1 and increased expression of VEGF gene in AML patients closely relate to the poor prognosis of AML patients, which can provide reference for improving clinical efficacy of AML patients.


Assuntos
Leucemia Mieloide Aguda , Fator de Crescimento Transformador beta1/genética , Fator A de Crescimento do Endotélio Vascular/genética , Humanos , Estimativa de Kaplan-Meier , Leucemia Mieloide Aguda/genética , Prognóstico , Indução de Remissão
9.
PeerJ ; 7: e7039, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31245175

RESUMO

Objective: Environmental endocrine disruptors (EEDs) with a weak ability to mimic estrogen have been associated with thyroid dysfunction. However, little is known about the effect of nonylphenol (NP), a well-known EED, on thyroid structure. The present study evaluates whether gestational and lactational exposure to NP impacts growth and thyroid structure in F1 male rats. Methods: A total of 60 rats were gavaged with NP (25, 50, and 100 mg/kg), estradiol (E2, 30 µg/kg/day), and corn oil alone (vehicle control) from gestational day 6 to postnatal day (PND) 21. Serum thyroid hormones free triiodothyronine (FT3), free thyroxine (FT4) and thyroid stimulating hormone levels were detected by automated chemiluminescence immunoassay analyzer. The NP level in the thyroid was measured using high-performance liquid chromatography. The ultrastructure of follicular epithelial cells was examined using transmission electron microscopy. Histopathology was conducted using hematoxylin and eosin staining. Results: On PND 0, exposure to 50 and 100 mg/kg/day NP led to a significant decrease in the average litter size, litter weight and number of live pups per litter compared to the control group (P < 0.05). Dams exposed to NP during perinatal period demonstrated decreased serum levels of FT3 and FT4 in F1 male rats, when compared to the control group (P < 0.05). The NP level in the control group was 3.39 ± 0.08 ng/mg, while NP levels in the low, middle, and high dose groups ranged from 5.20 to 11.00 ng/mg. Exposure caused a dose-related increase in NP level in the thyroid of male pups (P < 0.01). The thicknesses of the thyroid follicular epithelium were 2.06 ± 0.37 µm in the control group and 3.97 ± 1.61 µm in the high-dose group. The thickness of the thyroid follicular epithelium increased with an increase in treatment dose in a dose-dependent manner (P < 0.05). The sizes of the thyroid follicles were 1,405.53 ± 866.62 µm2 in the control group and 317.49 ± 231.15 µm2 in the high-dose group. With increasing NP dosages, animals showed a decreased size of the thyroid follicle (P < 0.01). Thyroid follicular cells of NP-treated rats showed mildly swollen mitochondria and dilated rough endoplasmic reticulum in the cytoplasm. Conclusion: Nonylphenol can cross the placental barrier and accumulate in the thyroid of F1 male rats. Gestational and lactational exposure to NP in dams impacted both development and growth of pups and damaged the ultrastructure of their thyroid tissue, which may further negatively influence normal thyroid function.

10.
Oncotarget ; 8(39): 65211-65217, 2017 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-29029424

RESUMO

AIM: Acute myeloid leukemia (AML) is the most common blood tumor with poor prognosis. At present, the research found that the pathogenesis of AML is related to many factors, such as recurrent somatic mutations and gene expression and epigenetic changes, however, the molecular mechanism of AML is still unclear. Long non-coding RNA MEG3 is a newly found tumor suppressor and plays a very important role in the regulation of a variety of tumor formation and progression. Studies found that the MEG3 expression was significantly decreased in AML. However, to date, it is not clear the cause of its abnormal expression. Therefore, the molecular mechanism of AML is urgently needed to study the molecular mechanism of AML. METHODS: The different expression level of MEG3, TET2, miR-22-3p, miR-22-5p in AML was detected by real-time quantification PCR. MEG3, TET2, miR-22-3p, miR-22-3p expression cell pools in K562 cells was used to interfering and TET2, MEG3 TET2, relations with miR-22-3p, miR-22-5p. The effect of AML cell on proliferation was evaluated by TET2 lower expression. RESULTS: 1. The lower expression of MEG3 and TET2 in AML cell lines was detected by RT-qPCR. 2. The stable MEG3, TET2 overexpression cell pools in K562 cells was successful established. 3. After transfection, MTT assay revealed that cell growth was significantly increased in AML cell lines transfected with TET2 compared with controls. CONCLUSIONS: Our findings suggested that MEG3 is significantly down regulated in AML cell lines.

11.
Mol Med Rep ; 15(6): 4266-4272, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28487941

RESUMO

The present study aimed to investigate the microRNA (miRNA) profile in human medullary thyroid carcinoma (MTC) tissue. The GSE40807 data profile was downloaded from the Gene Expression Omnibus database. Following preprocessing, differentially expressed microRNAs (DEMs) between MTC and healthy tissues were identified. Based on the obtained DEMs, transcription factor (TF)­miRNA and miRNA­target gene regulatory association pairs were predicted. Finally, functional enrichment analysis was performed on target genes of DEMs. Fifteen upregulated and 17 downregulated DEMs were identified. In the constructed TF­miRNA regulatory network, hsa­miR­9­5p was regulated by 9 TFs and hsa­miR­1 was regulated by 8 TFs. TFs of nuclear factor of κ light polypeptide gene enhancer in B­cells 1 (NF­κB1) and v­myc avian myelocytomatosis viral oncogene homolog (MYC) regulated 4 and 3 DEMs, respectively. In the miRNA­target gene regulatory network, hsa­miR­1, hsa­miR­9­5p, hsa­miR­96­5p and hsa­miR­590­5p were most upregulated. The target genes of these 4 miRNAs were primarily enriched in the mitogen activated protein kinase (MAPK) signaling pathway. Therefore, MAPK signaling pathway may serve important roles in MTC progression. In conclusion, the DEMs hsa­miR­1 and hsa­miR­9­5p, and TFs of NF­κB1 and MYC may be used as biomarkers for the diagnosis and treatment of MTC.


Assuntos
Carcinoma Neuroendócrino/genética , MicroRNAs/genética , Neoplasias da Glândula Tireoide/genética , Biomarcadores Tumorais/genética , Biologia Computacional/métodos , Regulação para Baixo/genética , Perfilação da Expressão Gênica/métodos , Regulação Neoplásica da Expressão Gênica/genética , Redes Reguladoras de Genes/genética , Genes myc/genética , Humanos , Proteínas Quinases Ativadas por Mitógeno/genética , NF-kappa B/genética , Transdução de Sinais/genética , Regulação para Cima/genética
12.
Oncotarget ; 8(11): 18337-18347, 2017 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-28407691

RESUMO

The promoter of MEG3, which encodes the long non-coding RNA (lncRNA) MEG3, is often hypermethylated in acute myeloid leukemia (AML). Additionally, the Tet methylcytosine dioxygenase 2 gene (TET2) is frequently inactivated, which can lead to impaired DNA methylation and promote AML development. We examined the association between TET2 and MEG3 promoter hypermethylation in Hainan patients with AML. The expression of MEG3, TET2, miR-22-3p, and miR-22-5p was assessed in bone marrow samples from AML patients and healthy controls using real-time quantitative PCR. Using Sequenom MassARRAY technology, we compared MEG3 promoter methylation in AML patients and healthy controls. MEG3 expression was lower in AML patients than in the controls (P = 0.136). Moreover, there was greater methylation of MEG3 promoter in the AML patients than the controls (P < 0.05). Methylation of the MEG3 promoter correlated negatively with TET2 expression (P < 0.05, r < 0). Likewise there was a negative correlation between TET2 activity and MEG3 promoter methylation (P < 0.05, r < 0). These results suggest that hypermethylation of the MEG3 promoter in AML may result from decreased TET2 activity. These data provide insight into the molecular mechanisms underlying AML development and progression.


Assuntos
Metilação de DNA , Proteínas de Ligação a DNA/genética , Leucemia Mieloide Aguda/genética , Proteínas Proto-Oncogênicas/genética , RNA Longo não Codificante/genética , Estudos de Casos e Controles , Linhagem Celular Tumoral , China , Proteínas de Ligação a DNA/metabolismo , Humanos , Leucemia Mieloide Aguda/metabolismo , MicroRNAs/biossíntese , MicroRNAs/genética , Regiões Promotoras Genéticas , Proteínas Proto-Oncogênicas/metabolismo , RNA Longo não Codificante/metabolismo
13.
Artigo em Chinês | MEDLINE | ID: mdl-26201191

RESUMO

OBJECTIVE: To investigate the effect of T lymphoma invasion and metastasis 1 (Tiam 1) overexpression in head and neck squamous cell carcinoma (HNSCC) cells. METHOD: Endogenous expression of Tiam 1 in 8 head and neck squamous cell carcinoma cell (HNSCC) lines was investigated by real-time RT-PCR. A lentivirus vector containing Tiaml was transfected into UM-SCC-47 cells, a head and neck squamous cell carcinoma cell line with little endogenous Tiaml expression. Stable clone, obtained by G418 screening, were assayed by RT-PCR and Western blot to validate the gene expression efficiency. The biological behaviors of the transduced cells were determined by cell counting, MTT and in-vitro migration assay. RESULT: Tiam 1 gene was highly expressed in M2 cell line and it's low level expression was found in UM-SCC-47. Cell counting and MTT assay showed that over-expression of Tiaml significantly promoted cell proliferation (P < 0.05). The cell monolayers overexpressed Tiaml that resulted in a significant increasment of cell migration in infected head and neck squamous cell carcinoma cell lines (P < 0.05). CONCLUSION: Tiam 1 gene plays an important role in the growth and migration in head and neck squamous cell carcinoma cell lines. It may be a useful marker for metastasis of head and neck squamous cell carcinoma.


Assuntos
Carcinoma de Células Escamosas/patologia , Fatores de Troca do Nucleotídeo Guanina/metabolismo , Neoplasias de Cabeça e Pescoço/patologia , Western Blotting , Carcinoma de Células Escamosas/metabolismo , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Fatores de Troca do Nucleotídeo Guanina/genética , Neoplasias de Cabeça e Pescoço/metabolismo , Humanos , Carcinoma de Células Escamosas de Cabeça e Pescoço , Proteína 1 Indutora de Invasão e Metástase de Linfoma de Células T , Transfecção
14.
Blood Cells Mol Dis ; 53(1-2): 16-20, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24534675

RESUMO

This study examines the frequency and spectrum of α- and ß-thalassemia (thal) mutations of the Li people in Hainan Province of China. We have analyzed by genotyping a sample of 8600 subjects of the Li people and found that 53.45% subjects have only α-thal mutations with high frequencies of -α(4.2) and -α(3.7), but fewer --(SEA) mutation; 3.83% have ß-thal mutations all identified to be 41/42 (-TCTT); whereas 7.99% carry both α-thal and ß-thal mutations. We also examined 9800 subjects of the Han people, and the result showed 12.16% subjects have only α-thal mutations with --(SEA) and -α(3.7) the most frequent mutation types, 6.11% have only ß-thal mutations of 7 types, whereas 4.85% carry both α-thal and ß-thal mutations. Our study demonstrated that the Li people in Hainan province have a high incidence of -α(4.2) and -α(3.7) thalassemia, low frequencies of α-thal -(SEA), and a novel ß mutation, 41/42 (-TCTT). We provide the complete spectrum of α-thal and ß-thal mutations and a strategy for accurate molecular diagnostic testing in the Li people in Hainan Province of Southern China.


Assuntos
Grupos Étnicos/genética , Mutação , Talassemia alfa/genética , Talassemia beta/genética , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Alelos , Criança , Pré-Escolar , China/epidemiologia , Frequência do Gene , Genótipo , Geografia , Humanos , Incidência , Lactente , Pessoa de Meia-Idade , Fenótipo , Adulto Jovem , alfa-Globinas/genética , Talassemia alfa/epidemiologia , Globinas beta/genética , Talassemia beta/epidemiologia
15.
Zhonghua Yi Xue Za Zhi ; 90(4): 253-5, 2010 Jan 26.
Artigo em Chinês | MEDLINE | ID: mdl-20356540

RESUMO

OBJECTIVE: To observe the influence of the plasma thromboxane B2 (TXB2), 6-keto-PGF1alpha, CD62P and PAC-1 and Thrombus in patients with primary thrombocytosis (ET). To observe the effect of sodium ozagrel to prevent and treat thrombosis in patients with ET. METHODS: The subjects including 48 patients with ET. All patients were measured the plasma TXB2, 6-keto-PGF1alpha, CD62P and PAC-1 before and after treatment with or without sodium ozagrel. RESULTS: The plasma levels of CD62P, PAC-1, TXB2, 6-keto-PGF1alpha and TXA2/PGI2 in the patients with ET were significantly higher than the normal people (P < 0.01). The levels of CD62P, PAC-1, TXB2, TXB2/6-keto-PGF1alpha in patients with treatment of sodium ozagrel were higher than patients without treatment of sodium ozagrel (P < 0.01). The plasma levels of CD62P, PAC-1 and TXA2/PGI2 in patients with treatment of sodium ozagrel and that in normal people had no significant distinction (P < 0.01). All the index of conventional therapy group were higher than normal people (P < 0.01) but had no significant distinction with the patients before conventional treating. The incidence of thrombus in patients treated with sodium ozagrel was lower than patients treated without sodium ozagrel (P < 0.05). CONCLUSION: With the treatment of sodium ozagrel in patients with ET, the CD62P, PAC-1, TXB2 and TXA2/PGI2 of plasma could be decreased. And the incidence of thrombus was decreased.


Assuntos
Anticorpos Monoclonais/sangue , Plaquetas/fisiologia , Metacrilatos/uso terapêutico , Trombocitemia Essencial/fisiopatologia , Trombose/prevenção & controle , 6-Cetoprostaglandina F1 alfa/sangue , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Selectina-P/sangue , Receptores de Fibrinogênio/imunologia , Receptores de Polipeptídeo Hipofisário Ativador de Adenilato Ciclase/sangue , Trombose/tratamento farmacológico , Tromboxano A2/sangue , Tromboxano B2/sangue
16.
Artigo em Chinês | MEDLINE | ID: mdl-19685714

RESUMO

OBJECTIVE: To investigate the effects of radioactive ray on transmission capability of nose mucociliary. METHOD: Forty-six patients with NPC were selected and saccharin clearance time (SCT) for 7 phases were detected in both pre- and post-radiotherapy respectively. RESULT: Among 46 patients with NPC, the shortest SCT was 247 seconds and the longest 601 seconds in pre-radiotherapy phases; from 4th week of introradiotherapy to 18 months of postradiotherapy, the longest SCT was in 12 months after radiotherapy, which was 903 seconds. There were no significant differences in SCT before radiotherapy and 18 months after radiotherapy. There were significant differences in SCT of preradiotherapy and introdiotherapy, post radiotherapy, after radiotherapy 3 months, 6 months, 12 months after radiotherapy. CONCLUSION: Radiotherapy is the important factors in influencing transmission capability of nose cavity and sinus mucociliary and hints that gender and nasal cavity side don't affect SCT. Detection of SCT in different stages of NPC patients can be helpful to protect nasal mucous membrane effectively, and to reduce incidence rate of RNS.


Assuntos
Depuração Mucociliar/efeitos da radiação , Mucosa Nasal/fisiopatologia , Neoplasias Nasofaríngeas/fisiopatologia , Neoplasias Nasofaríngeas/radioterapia , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Sacarina/metabolismo , Adulto Jovem
17.
Lin Chuang Er Bi Yan Hou Ke Za Zhi ; 18(9): 553-4, 2004 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-15696957

RESUMO

OBJECTIVE: To explore the diagnosis and treatment of maxillary aneurysmal bone cyst. METHOD: For 4 cases with maxillary aneurysmal bone cyst, 1 case underwent radical maxillary sinusotomy in local anaesthesia and 3 cases underwent lateral rhinotomy in general anaestesia. RESULT: The 4 cases were followed up for 13 months to 2 years after operation and no patient was found recurrence. CONCLUSION: The main treatment of aneurysmal bone cyst was radical operation, to strive for thorough treatment. Confirming and embolizing the feed artery by DSA preoperatively can reduce bleeding during operation and shorten the time of operation and general anesthesia obviously.


Assuntos
Cistos Ósseos Aneurismáticos/cirurgia , Maxila/cirurgia , Adolescente , Cistos Ósseos Aneurismáticos/diagnóstico , Criança , Feminino , Seguimentos , Humanos , Masculino , Maxila/patologia
18.
Zhonghua Er Bi Yan Hou Ke Za Zhi ; 39(12): 730-2, 2004 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-15813015

RESUMO

OBJECTIVE: To study the incidence, clinical features and related factors of nasosinusitis after radiotherapy for nasopharyngeal carcinoma. METHODS: Five hundred and thirteen patients with nasopharyngeal carcinoma were included in the study, to observe the clinical manifestation and image changes before and after radiotherapy. The incidence and influencing factors of nasosinusitis after radiotherapy were analyzed. RESULTS: Among 513 patients, before radiotherapy, nasosinusitis was found in 51 patients (9.9%). After radiotherapy, another 401 nasosinusitis was found (401/462). The difference of incidence rate of nasosinusitis before and after radiotherapy was obvious (chi2 = 533.21, P < 0.01). The incidence rate of nasosinusitis in the end of radiotherapy, 3 months, 6 months, 12 months and 18 months after radiotherapy was 10.7% (43/401), 13.7% (55/401), 58.1% (233/401), 12.0% (48/401), 5.5% (22/401) respectively. The incidence rate of nasosinusitis after fractional radiotherapy and continuous radiotherapy was 35.7% (143/401), 64.3% (258/401) respectively. CONCLUSION: The incidence rate of nasosinusitis after radiotherapy is very high. It is influenced by the dose of radiotherapy, but it has no relation with the extension of nasopharyngeal carcinoma.


Assuntos
Neoplasias Nasofaríngeas/radioterapia , Sinusite/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma , Causalidade , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Carcinoma Nasofaríngeo , Neoplasias Nasofaríngeas/patologia , Radioterapia (Especialidade) , Dosagem Radioterapêutica , Adulto Jovem
19.
Lin Chuang Er Bi Yan Hou Ke Za Zhi ; 17(12): 739-41, 2003 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-15017724

RESUMO

OBJECTIVE: To examine the expression of apoptosis-related genes c-IAP2 and caspase-4 in head and cervical undifferentiation cell carcinoma, as well as to investigate the role and mechanism of the two apoptosis-related genes in head and cervical undifferentiation squamous cell carcinoma. METHOD: Sixty-six historily confirmed specimen of head and cervical undifferentiation squamous cell carcinoma were subjected for immunohistochemical staining to analyze the expression of c-IAP2 and caspase-4, in addition 5 cases of normal nasal mucosa tissue were selected as control. RESULT: 1. Higher level expression of c-IAP2 protein was detected in the head and cervical undifferentiation squamous cell carcinoma specimen compared with normal nasal mucosa(P < 0.05), and the expression of caspase-4 was more deletion in it than in normal nasal mucosa(P < 0.05). 2. The expression of c-IAP2 protein was positive correlation with clinical prognosis, not correlated with clinical staging. The expression of caspase-4 protein was negative correlated with clinical prognosis, not correlated with clinical staging, too. 3. The expression of c-IAP2 was negative correlated with the expression of caspase-4. CONCLUSION: It indicates that in tissue of head and cervical undifferentiation squamous cell carcinoma, c-IAP2 is activated while caspase-4 is restrained. The signal transduction pathway of apoptosis is arrested, leading to surviving of the tumor cells, which maybe directly associated with the developing of head and cervical undifferentiation squamous cell carcinoma, and also with the failure of tumor treatment.


Assuntos
Apoptose , Carcinoma de Células Escamosas/metabolismo , Caspases/biossíntese , Neoplasias de Cabeça e Pescoço/metabolismo , Biossíntese de Proteínas , Caspases/genética , Caspases Iniciadoras , Neoplasias Laríngeas/metabolismo , Neoplasias Nasofaríngeas/metabolismo , Prognóstico , Proteínas/genética
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