Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 7 de 7
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
BMC Infect Dis ; 19(1): 1042, 2019 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-31823777

RESUMO

BACKGROUND: Toxoplasma gondii is an opportunistic protozoan parasite that can infect all warm-blooded animals including humans and cause serious clinical manifestations. Toxoplasmosis can be diagnosed using histological, serological, and molecular methods. In this study, we aimed to detect T. gondii RE gene in various human samples by in house and commercial real time polymerase chain reactions. METHODS: A total of 38 suspected cases of toxoplasmosis [peripheral blood (n:12), amnion fluid (n:11), tissue (n:9), cerebrospinal fluid (n:5), and intraocular fluid (n:1)] were included to the study. An in house and a commercial RT-PCR were applied to investigate the T. gondii RE gene in these samples. RESULTS: The compatibility rate of the two tests was 94.7% (37/38). When the commercial RT-PCR kit was taken as reference, the sensitivity and specificity of in house RT-PCR test was 87.5 and 100%. When the in house RT-PCR test was taken as reference, the commercial RT-PCR kit has 100% sensitivity and 96.8% specificity. Incompatibility was detected in only in a buffy coat sample with high protein content. CONCLUSIONS: Both the commercial and in house RT-PCR tests can be used to investigate T. gondii RE gene in various clinical specimens with their high sensitivity and specificity. In house RT-PCR assay can be favorable due to cost savings compared to using the commercial test.


Assuntos
DNA de Protozoário/metabolismo , Reação em Cadeia da Polimerase em Tempo Real/métodos , Toxoplasma/genética , Líquido Amniótico/microbiologia , Animais , Buffy Coat/microbiologia , DNA de Protozoário/isolamento & purificação , Humanos , Masculino , Kit de Reagentes para Diagnóstico , Toxoplasma/isolamento & purificação , Toxoplasmose/diagnóstico , Toxoplasmose/microbiologia , Turquia
2.
Turkiye Parazitol Derg ; 42(3): 237-239, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30280697

RESUMO

Toxoplasma gondii is an obligatory intracellular protozoon parasite that causes toxoplasmosis in humans and all warm-blooded animals. In this study, we aimed to investigate the presence of T. gondii DNA in a Eurasian badger (Meles meles) that was found dead in the wildlife area of Izmir. According to the results of real time polymerase chain reaction, T. gondii REP gene was found to be positive in the Eurasian badger brain homogenate. In conclusion, Eurasian badger, a known carnivore, can be a potential source of toxoplasmosis in the natural settings of Izmir, Turkey.


Assuntos
Mustelidae , Toxoplasma/isolamento & purificação , Toxoplasmose Animal/diagnóstico , Animais , Animais Selvagens , DNA de Protozoário/análise , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Toxoplasma/genética , Toxoplasmose Animal/parasitologia , Turquia
3.
Parasit Vectors ; 11(1): 393, 2018 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-29973272

RESUMO

BACKGROUND: Toxoplasma gondii is an obligate intracellular protozoan parasite that causes congenital toxoplasmosis, as well as other serious clinical presentations in immune compromised humans. The parasite has also been recently linked to behavioral diseases in humans and other mammalian hosts. New antigens are being evaluated to develop a diagnostic kit for the diagnosis of acute infection or a protective vaccine. METHODS: In this study, we have focused on the discovery of new antigenic proteins from T. gondii genomic data using a high throughput protein microarray screening. To date, microarrays containing > 2870 candidate exon products of T. gondii have been probed with sera collected from patients with toxoplasmosis. Here, the protein microarrays are probed with well-characterized serum samples from animal models administered orally with oocysts or tissue cysts. The aim was to discover the antigens that overlap in the mouse profile with human antibody profiles published previously. For this, a reactive antigen list of 240 antigens recognized by murine IgG and IgM was identified using pooled sera from orally infected mice. RESULTS: Analyses of screening data have identified plenty of antigens and showed strong immunogenicity in both mouse and human antibody profiles. Among them, ROP1, GRA2, GRA3, GRA4, GRA5, GRA6, GRA7, GRA8, GRA14, MIC1, MIC2 and MAG1 have shown strong immunogenicity and used as antigen in development of vaccines or serological diagnostic assays in previous studies. CONCLUSION: In addition to the above findings, ROP6, MIC12, SRS29A and SRS13 have shown strong immunogenicity but have not been tested in development of a diagnostic assay or a vaccine model yet.


Assuntos
Anticorpos Antiprotozoários/sangue , Antígenos de Protozoários/isolamento & purificação , Oocistos/imunologia , Análise Serial de Proteínas , Proteínas de Protozoários/isolamento & purificação , Toxoplasma/química , Administração Oral , Animais , Antígenos de Protozoários/genética , Antígenos de Protozoários/imunologia , Modelos Animais de Doenças , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imunoglobulina G/sangue , Camundongos , Proteínas de Protozoários/imunologia , Testes Sorológicos , Toxoplasma/genética , Toxoplasma/imunologia , Toxoplasmose/sangue , Toxoplasmose/diagnóstico , Toxoplasmose/imunologia
4.
PLoS One ; 13(4): e0196159, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29668747

RESUMO

Toxoplasma gondii is a protozoon parasite that causes congenital toxoplasmosis, as well as other serious clinical presentations, in immune compromised humans. Analyses of the prevalence and genotyping of strains from the definitive host and intermediate hosts will help to understanding the circulation of the different strains and elucidating the role of the genotype(s) in human toxoplasmosis. Turkey has a specific geographic location bridging Africa, Europe, and Asia. We hypothesized that T. gondii strains may have been transferred to Turkey from these continents via migratory birds or vice versa. The present study aimed to assess the prevalence of toxoplasmosis in wild birds of prey of Izmir and Manisa provinces as well as genetically characterize T. gondii strains from these wild birds to show the relation between bird strains and neighboring stray cats as well as human strains previously isolated in Turkey. Tissues obtained from 48 wild birds were investigated for the presence of T. gondii DNA and then bioassayed in mouse. Isolated strains were genotyped using 15 microsatellite markers. The prevalence of T. gondii DNA was found to be 89.6% (n: 43/48) in wild birds. Out of 43 positive samples, a total of 14 strains were genotyped by 15 microsatellite markers. Among them, eight were type II, three were type III and three were mixture of genotypes (two type II/II and one was II/III). These are the first data that showed the presence of T. gondii and types II and III genotypes in wild birds of Turkey. Moreover, Africa 1 was not detected. In addition, cluster analysis showed that T. gondii strains within type II and III lineage have close relation with strains previously isolated from stray cats in Izmir. Further studies are required to isolate more strains from human cases, other intermediate hosts, and water sources to reveal this relation.


Assuntos
Animais Selvagens , Aves/parasitologia , Genótipo , Toxoplasma/classificação , Toxoplasma/genética , Toxoplasmose Animal/epidemiologia , Toxoplasmose Animal/parasitologia , Animais , Modelos Animais de Doenças , Variação Genética , Geografia , Camundongos , Repetições de Microssatélites , Tipagem Molecular , Filogenia , Toxoplasma/isolamento & purificação , Turquia/epidemiologia
5.
Exp Parasitol ; 183: 137-142, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28807756

RESUMO

Acanthamoeba is a free-living amoeba which can be isolated from environment and among others well known as an opportunist protozoan parasite causing infections in humans and animals. Eyes are extremely important for the wild birds and losing sight ability due to Acanthamoeba can be dangerous. The studies on Acanthamoeba infection in wild birds is very few in world and Turkey therefore we aimed to screen deceased wild birds found in Izmir and Manisa provinces located in western Turkey using PCR and non-nutrition agar (NNA) plate method. Cornea samples were obtained from 18 deceased wild birds. During the external examination, signs of keratitis were observed in two Eurasian sparrowhawks (Accipiter nisus). All of the corneal samples were analyzed by two PCR methods and NNA plate. According to results, the Acanthamoeba positivity in corneal samples was 16.6% and 5.5% by PCR and plate method, respectively. According to sequencing data, two of isolates belonged to genotype T5 and one was genotype T4. In conclusion, Acanthamoeba infection was detected in wild bird cornea samples with/without keratitis for the first time in the world. The result of this study also show that Acanthamoeba can be a cause of keratitis in wild birds of Turkey and thus these predator birds can be a target of other wild animals due to loss of sight ability. In terms of public health, these results show the importance of wild birds as a source of Acanthamoeba infection in nature.


Assuntos
Ceratite por Acanthamoeba/veterinária , Acanthamoeba/isolamento & purificação , Doenças das Aves/parasitologia , Córnea/parasitologia , Acanthamoeba/classificação , Acanthamoeba/genética , Ceratite por Acanthamoeba/patologia , Ceratite por Acanthamoeba/fisiopatologia , Animais , Animais Selvagens , Doenças das Aves/patologia , Doenças das Aves/fisiopatologia , Aves , Córnea/patologia , DNA de Protozoário/química , DNA de Protozoário/isolamento & purificação , Genótipo , Reação em Cadeia da Polimerase/métodos , Reação em Cadeia da Polimerase/veterinária , RNA Ribossômico 18S/genética , Turquia
6.
Turk J Med Sci ; 47(3): 897-901, 2017 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-28618740

RESUMO

BACKGROUND/AIM: Strongyloides stercoralis causes life-threatening hyperinfection or disseminated strongyloidiasis in immunocompromised patients such as HIV-positive, organ transplantation, and cancer patients. This study investigated the presence of strongyloidiasis in immunocompromised patients for the first time in Turkey. MATERIALS AND METHODS: Serum and stool samples were collected from 108 patients (25.9% of them were chronic renal failure and 74.1% were renal transplantation patients) who were admitted to Ege University Medical School in Izmir, located in western Turkey. Serum samples were analyzed by ELISA (DRG, Germany) and the presence of 18S rRNA gene of S. stercoralis was detected in stool samples by real-time PCR. RESULTS: The analysis of serum samples showed that only one patient was anti-S. stercoralis IgG antibody and real-time PCR positive (0.92%). The patient was treated twice with albendazole (400 mg/day for 3 days) at 2-week intervals. Follow up real-time PCR was negative and the patient became seronegative 6 months after the initial diagnosis. CONCLUSION: This screening showed that the prevalence of strongyloidiasis in this small group of patients who were at risk of strongyloidiasis was 0.92%. Overall, the results showed that more systematic studies are required in Turkey to show the prevalence of strongyloidiasis.


Assuntos
Ensaio de Imunoadsorção Enzimática/métodos , Hospedeiro Imunocomprometido , Imunossupressores/efeitos adversos , Reação em Cadeia da Polimerase em Tempo Real/métodos , Estrongiloidíase/diagnóstico , Animais , Anticorpos Anti-Helmínticos/sangue , Estudos de Coortes , Rejeição de Enxerto/tratamento farmacológico , Rejeição de Enxerto/prevenção & controle , Humanos , Imunossupressores/uso terapêutico , Falência Renal Crônica , Transplante de Rim , Strongyloides/genética , Strongyloides/imunologia , Turquia
7.
Turkiye Parazitol Derg ; 41(4): 233-238, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29318997

RESUMO

Travel-related health problems have been reported in 22-64% of travelers to developing countries. Approximately 8% of these patients are moderately ill and are referred to health facilities. Post-travel infections are usually symptomatic in the early stages, but they may last for up to months or even years, depending on the incubation period. It has been reported that it is not necessary to have extensive knowledge on tropical diseases to be able to make a clinical evaluation after the trip. All post-travel consultations should be performed by physicians and should include travel-related illness identification, timely medical intervention, and, if necessary, referral to a senior hospital. Situations that should be taken into consideration by physicians when evaluating a possible patient with travel-related health problems are as follows: the severity of illness, the route travelled, the time between illness and travel, the underlying disease, vaccine and prophylaxis history, and exposure history. The most common clinical syndromes after travel to developing countries are systemic febrile illness, acute diarrhea, dermatological disorders, respiratory disorders, and eosinophilia. This review summarizes the approach to possible clinical situations among returned travelers.


Assuntos
Diarreia/diagnóstico , Padrões de Prática Médica , Medicina de Viagem , Doença Relacionada a Viagens , Países em Desenvolvimento , Diarreia/complicações , Febre/etiologia , Humanos , Turquia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA