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1.
Front Microbiol ; 13: 816631, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35185847

RESUMO

OBJECTIVES: For patients with intra-abdominal infection (IAI), the rapid and accurate identification of pathogens remains a challenge. Metagenomic next-generation sequencing (mNGS) is a novel technique for infectious diseases, but its application in IAI is limited. In this study, we compared the microbiological diagnostic ability of plasma mNGS with that of conventional peritoneal drainage (PD) culture in critical care settings. METHODS: From January 2018 to December 2020, a prospective observational study was performed at a tertiary teaching hospital in China and data on 109 abdominal sepsis patients were collected. The pathogen detection performance of plasma mNGS and PD culture method were compared. MEASUREMENTS AND RESULTS: Ninety-two positive cases detected on PD culture, while plasma mNGS detected 61 positive cases. Forty-five patients (44.0%) had at least one matched pair of plasma mNGS and PD culture results. Compared with PD culture, the plasma mNGS was more rapid (27.1 ± 4.0 vs. 68.9 ± 22.3 h, p < 0.05). The patients received initial antibiotic treatment matched with mNGS detection showed better clinical outcomes. CONCLUSION: For abdominal sepsis patients, plasma mNGS can provide early, noninvasive, and rapid microbiological diagnosis. Compared with conventional PD smear, culture, and blood culture methods, plasma mNGS promote the rapid detection of pathogenic bacteria.

2.
EBioMedicine ; 74: 103649, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34814051

RESUMO

BACKGROUND: Shotgun metagenomics has been used clinically for diagnosing infectious diseases. However, most technical assessments have been limited to individual sets of reference standards, experimental workflows, and laboratories. METHODS: A reference panel and performance metrics were designed and used to examine the performance of shotgun metagenomics at 17 laboratories in a coordinated collaborative study. We comprehensively assessed the reliability, key performance determinants, reproducibility, and quantitative potential. FINDINGS: Assay performance varied significantly across sites and microbial classes, with a read depth of 20 millions as a generally cost-efficient assay setting. Results of mapped reads by shotgun metagenomics could indicate relative and intra-site (but not absolute or inter-site) microbial abundance. INTERPRETATION: Assay performance was significantly impacted by the microbial type, the host context, and read depth, which emphasizes the importance of these factors when designing reference reagents and benchmarking studies. Across sites, workflows and platforms, false positive reporting and considerable site/library effects were common challenges to the assay's accuracy and quantifiability. Our study also suggested that laboratory-developed shotgun metagenomics tests for pathogen detection should aim to detect microbes at 500 CFU/mL (or copies/mL) in a clinically relevant host context (10^5 human cells/mL) within a 24h turn-around time, and with an efficient read depth of 20M. FUNDING: This work was supported by National Science and Technology Major Project of China (2018ZX10102001).


Assuntos
Bactérias/isolamento & purificação , Doenças Transmissíveis/diagnóstico , Fungos/isolamento & purificação , Metagenômica/instrumentação , Metagenômica/métodos , Bactérias/classificação , Bactérias/genética , Benchmarking , China , Fungos/classificação , Fungos/genética , Células HeLa , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Laboratórios , Metagenômica/normas , Reprodutibilidade dos Testes , Análise de Sequência de DNA , Fluxo de Trabalho
3.
Emerg Infect Dis ; 27(10): 2691-2694, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34545782

RESUMO

We describe 3 similar cases of rickettsial disease that occurred after tick bites in a mountainous rural area of Shandong Province, China. Next-generation sequencing indicated the etiologic agent of 1 patient was Rickettsia conorii subspecies indica. This agent may be more widely distributed across China than previously thought.


Assuntos
Febre Botonosa , Infecções por Rickettsia , Rickettsia conorii , Rickettsia , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Rickettsia/genética , Infecções por Rickettsia/diagnóstico , Rickettsia conorii/genética
4.
ACS Omega ; 6(25): 16488-16497, 2021 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-34235320

RESUMO

Aluminum hydroxide is an effective defluoridation adsorbent; however, the poor defluoridation performance limits its wide application. In this work, amorphous and crystalline AlOOH adsorbents are synthesized through hydrolysis of Al salts, and their defluoridation performances are evaluated in terms of adsorption capacity and rate, sensitivity to pH value, and water quality after defluoridation. The defluoridation performance of AlOOH is closely related to the hydrolysis pH value, but hardly to the type of Al salts. The adsorbent can remove >95% fluoride in the first 2 min and reach adsorption equilibrium within 2 h, and the maximum defluoridation capacity is 41.9 mg/g. Furthermore, the adsorbent exhibits an excellent defluoridation efficiency at a wide pH range of 4.5-10.5. After fluoride removal, the adsorbents prepared at pH values of 6 and 7 exhibit low residual Al concentration. The Fourier transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS) results confirm that the fluoride removal mechanism is the ligand exchange between fluoride and hydroxyl groups. The excellent defluoridation capacity and low residual Al demonstrate that AlOOH is a potential adsorbent for fluoride separation from water.

5.
Sci Total Environ ; 795: 148786, 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34229237

RESUMO

Bromate, a toxic by-product of bromide-containing drinking water after disinfecting with ozone, has attracted much attention in the past two decades. Traditional methods to activate zero-valent metals for reducing bromate are to eliminate their surface oxide layer by acid washing. In this work, for the first time, zero-valent Al (ZVAl) was surface treated by the following procedures including soaking, soaking and freeze-drying, soaking and heat-treating, and γ-Al2O3 covering Al particle surfaces (GCAP). It was found that all of above surface treated ZVAls have an obvious high efficiency for bromate reduction relative to pristine ZVAl. The bromate reduction rate is GCAP > soaking Al > freeze-drying Al > soaking and heat-treating Al > pristine Al, and using GCAP just 30 min is taken to completely reduce bromate to bromide in neutral solution. Mechanism analyses revealed that Al surface treating or covered by fine γ-Al2O3 phase can promote the hydration and breakage of Al surface passive oxide layer, resulting in a fast contact of inner Al with outside ions, leading to a high reduction rate of bromate in neutral solution. XPS analyses indicated that there are no bromate or bromide ions adsorbed on Al particle surfaces, implying that there is a high direct donating efficiency of electrons from inner Al to bromate ions in solution. Furthermore, GCAP has a good reusability and >90% bromate can be reduced even it was reused up to 4 cycles.


Assuntos
Poluentes Químicos da Água , Purificação da Água , Alumínio , Bromatos , Brometos , Oxirredução
6.
Clin Infect Dis ; 73(1): e246-e251, 2021 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-33146713

RESUMO

BACKGROUND: Alveolar echinococcosis (AE) is a serious parasitic disease caused by the larvae of Echinococcus multilocularis. It is the less common but substantially more deadly of the 2 major echinococcosis diseases that can occur globally but are concentrated in central Asia. METHODS: We analyzed parasite circulating cell-free DNA (cfDNA) in 149 plasma samples using a DNA sequencing-based method (105 AE, 16 cystic echinococcosis, 4 liver cancer, 4 gallstones, and 20 healthy volunteers). After identifying the Echinococcus-specific cfDNA (Em-cfDNA) sequences in the samples, we determined whether Em-cfDNA could be used for AE diagnosis and as a potential indicator of the effectiveness of surgical treatment. We also examined potential associations between Em-cfDNA levels and clinical features of AE patients. RESULTS: Our work demonstrates that varying reads of Em-cfDNA were detectable in the plasma of 100% of preoperative AE patients and that all of the non-AE patients and healthy volunteers were negative. Em-cfDNA has good sensitivity and specificity for the diagnosis of AE. We also found that Em-cfDNA levels apparently have reference value for evaluating the therapeutic efficacy of surgery interventions for AE lesions. Finally, our analysis revealed that Em-cfDNA levels can reflect meaningful information about lesion size in preoperative AE patients. CONCLUSIONS: We demonstrate that sequencing-based monitoring of Em-cfDNA can be used in the clinic as a powerful diagnostic indicator for AE. We also note that there is a strong potential for use of this liquid-biopsy method to monitor ongoing disease status in postintervention AE patients.


Assuntos
Ácidos Nucleicos Livres , Equinococose , Echinococcus multilocularis , Parasitos , Animais , Equinococose/diagnóstico , Echinococcus multilocularis/genética , Humanos
7.
Sensors (Basel) ; 20(4)2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-32075274

RESUMO

Although there are many algorithms to track people that are walking, existing methods mostly fail to cope with occluded bodies in the setting of multi-person tracking with one camera. In this paper, we propose a method to use people's shadows as a clue to track them instead of treating shadows as mere noise. We introduce a novel method to track multiple people by fusing shadow data from the RGB image with skeleton data, both of which are captured by a single RGB Depth (RGB-D) camera. Skeletal tracking provides the positions of people that can be captured directly, while their shadows are used to track them when they are no longer visible. Our experiments confirm that this method can efficiently handle full occlusions. It thus has substantial value in resolving the occlusion problem in multi-person tracking, even with other kinds of cameras.


Assuntos
Reconhecimento Automatizado de Padrão , Fotografação/instrumentação , Algoritmos , Humanos , Movimento (Física) , Fatores de Tempo
8.
RSC Adv ; 11(2): 867-875, 2020 Dec 24.
Artigo em Inglês | MEDLINE | ID: mdl-35423697

RESUMO

In this work, pristine aluminum (Al) powder was soaked in deionized water for a time period and then it was dried and heat-treated at 400 °C such that a layer of fine Al2O3 grains covered the Al particle surfaces, forming oxide modified Al powder (OM-Al). It was found that OM-Al greatly enhanced the efficiency in removing methyl orange (M-orange) and methyl blue (M-blue) in aqueous solution. The time to completely degrade M-orange and M-blue by OM-Al is about one third of that by pristine Al powder, and decreases with increasing dosage of OM-Al. The enhancement in dye removal rate by oxide modification is much better than that with ultrasonic assistance, especially for M-blue. LC/MS spectrum analyses revealed that large dye molecules are broken into small biodegradable organic molecules after reaction with OM-Al. It is deduced that the promotion of fine Al2O3 on the hydration process of Al surface passive oxide film is the main mechanism responsible for the enhancement of dye removal by OM-Al. Furthermore, OM-Al has a good recyclability and 80% of M-orange and M-blue can be removed even when it was reused for up to three cycles. These results indicate that oxide modification is an effective way to activate Al for the removal of organic dyes.

9.
Infect Drug Resist ; 12: 2467-2470, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31496760

RESUMO

A lung abscess is an infectious pulmonary disease characterized by pus-filled cavity formation and often an air-fluid level. In this article, we described an indolent community-acquired lung abscess suspected as a tumor previously. A 56-year-old male presented with cough and expectoration for 2 months and hemoptysis for 2 weeks. His physical examinations, whole blood count and C-reactive protein level were normal. The chest computed tomography (CT) scan showed a 40×38×39 mm high-density mass in the right upper pulmonary lobe, with irregular borders. The pathology of a CT-guided percutaneous needle aspiration biopsy showed numerous inflammatory cells and bacteria infiltration without tumor lesions. Bacteriological detection of lung tissue revealed the cause was odontogenic flora. A next-generation sequencing demonstrated the etiologic correlation between lung abscess and periodontitis. After a 2-month pathogen-directed oral antibiotics therapy combined with chlorhexidine gargle oral care, this patient showed a remarkable improvement. Periodontitis can be a cause of a lung abscess, which would be taken into account in the treatment regimes preventing infectious recurrence.

10.
BMC Cancer ; 19(1): 893, 2019 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-31492157

RESUMO

AIMS: To evaluate the short- and long-term outcomes of 3 different endoscopic dissection techniques for upper gastrointestinal (GI) submucosal tumours (SMTs). METHODS: Data for 135 patients withGI SMTs who underwent multiband mucosectomy (MBM), endoscopic submucosal dissection (ESD), or endoscopic submucosal excavation (ESE) were retrospectively assessed. The en bloc resection rate, endoscopic complete resection rate, operation time, potential complications and local recurrence rate were compared. RESULTS: No significant differences were observed in the rate of endoscopic complete resections and pathologic complete resections among the three groups. For SMTs > 15 mm in width, the lowest en bloc resection rate was found for MBM (P = 0.000). MBM was also associated with the shortest procedure time, lowest perforation rate and lowest rate of major bleeding. ESE was the most effective procedure for muscularis propria (MP) lesions but was associated with the longest operation time (P < 0.01). The ESD and ESE groups had similar perforation rates (P > 0.05). No differences were observed in 4-year local recurrence rates among the groups (P = 0.945). CONCLUSIONS: MBM is a simple and effective method for the treatment of small SMTs and achieves clinical success rates similar to those of ESD and ESE. However, ESD and ESE are preferable for larger and deep lesions and are associated with a longer operation time. Nonetheless, all 3 techniques resulted in a low 4-year local recurrence rate. Large-scale randomized clinical trials are needed to further investigate these results.


Assuntos
Ressecção Endoscópica de Mucosa/métodos , Neoplasias Gastrointestinais/cirurgia , Ressecção Endoscópica de Mucosa/efeitos adversos , Mucosa Esofágica/patologia , Mucosa Esofágica/cirurgia , Feminino , Seguimentos , Mucosa Gástrica/patologia , Mucosa Gástrica/cirurgia , Neoplasias Gastrointestinais/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia , Duração da Cirurgia , Complicações Pós-Operatórias/etiologia , Estudos Retrospectivos , Resultado do Tratamento
11.
Infect Drug Resist ; 12: 1073-1079, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31213854

RESUMO

Background: Mycoplasma is an opportunistic pathogen causing both urogenital and extragenital infections. The lack of cell wall renders Mycoplasma difficult to culture and identify with ordinary methods. Next-generation sequencing (NGS) is a new technology helping a lot in the diagnosis of infective diseases. In this case, NGS played a key role in the diagnosis of Mycoplasma infection. Case presentation: A mid-aged man suffering from renal cyst underwent cyst incision followed by invasive treatments to eliminate hematoma caused by renal artery hemorrhage. After the cyst incision operation, the patient had a persistent high temperature. The persistent increase of blood neutrophile granulocyte count and C-reaction protein suggested an unresolved infection. The empirically chosen anti-infective agents were meropenem and linezolid since the ordinary bacterial cultures of surgical site drainage and blood yielded a negative result. At postoperation day (POD) 17, NGS result of his drainage clearly indicated the pathogen was Mycoplasma hominis. At POD 24, the drug sensitivity test showed resistance to quinolones, clarithromycin and erythromycin, but intermediate to azithromycin. Since then, the antimicrobial agents were changed into azithromycin and kept unchanged until the patient was fully recovered and discharged at POD 39. Conclusion: When the ordinary laboratory diagnostic methods failed, NGS diagnosis could reduce the hospitalization expenses and shorten the lengths of hospital stay.

12.
AMB Express ; 9(1): 77, 2019 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-31139958

RESUMO

We introduced a novel micro/nanofluidic chip platform (MNCP), which is based on an isothermal nucleic acid amplification method. This study aimed to evaluate the MNCP method for influenza A and B viruses detecting and subtyping using throat swab samples from patients with influenza-like illness (ILI). A total of 266 throat swab samples from 266 non-repeated patients with ILI were tested for influenza A and B viruses using three methods, MNCP, a rapid influenza diagnostic test (RIDT), and real-time reverse transcription polymerase chain reaction (rRT-PCR). The results of MNCP were compared to those obtained by rRT-PCR and RIDT and the performance of MNCP was further evaluated. Compared with rRT-PCR results, the rates of sensitivity, specificity, overall concordance, and the kappa value of MNCP were 98.89%, 96.97%, 97.65%, and 0.95 for influenza A virus; 94.95%, 99.38%, 97.68%, and 0.95 for influenza B virus, respectively. Subtypes of influenza A viruses, e.g., A(H1N1)pdm09, A(H3N2), and A(not subtyped), and influenza B viruses could be distinguished in one MNCP assay within 1 h. Compared with rRT-PCR and MNCP, RIDT showed poor clinical sensitivity for influenza virus detection. This study showed MNCP is rapid, sensitive and versatile detecting system with potential for clinical application in pathogen diagnosis for patients with ILI.

13.
Cells Tissues Organs ; 208(1-2): 13-24, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-32045921

RESUMO

OBJECTIVE: The aim of this work was to determine the effect of miR-375 on chondrocyte metabolism and oxidative stress in osteoarthritis (OA) mouse models through the JAK2/STAT3 signaling pathway. METHODS: Chondrocytes were divided into control, IL-1ß, IL-1ß + miR-375 mimic, IL-1ß + miR-375 inhibitor, IL-1ß + miR-NC (negative control), and IL-1ß + miR-375 inhibitor + siJAK2 groups. The chondrocyte proliferation was determined by MTT assay, the superoxide dismutase (SOD) and malondialdehyde (MDA) levels by corresponding kits, and the chondrocyte apoptosis by TUNEL staining. Furthermore, OA mouse models were divided into Sham, OA + miR-NC, and OA + miRNA-375 antagomir groups. The pathological changes were observed, and the expressions of miR-375 and the JAK2/STAT3 pathway were determined by qRT-PCR and Western blotting, respectively. RESULTS: IL-1ß-induced chondrocytes had significant increases in miR-375 and MDA, with decreased proliferation and SOD levels, as compared to the control group. Meanwhile, they also exhibited elevated apoptosis, with upregulations of ADAMTS-5 and MMP-13 and downregulations of COL2A1 and ACAN, as well as decreased p-JAK2/JAK2, p-STAT3/STAT3, and Bcl-2/Bax. However, these changes were significantly improved after transfection with miR-375 inhibitor, but transfection with miR-375 mimic resulted in severer exacerbation. Notably, the improvement of miR-375 inhibitor could be abolished by transfection with siJAK2. Furthermore, miR-375 antagomir significantly alleviated OA progression in OA mice in vivo. CONCLUSION: MiR-375 suppression enhanced the ability of chondrocyte to antagonize the oxidative stress and maintained the homeostasis of extracellular matrix metabolism to protect chondrocytes from OA via activation of the JAK2/STAT3 pathway, indicating that miR-375 is a potential molecular target for OA treatment.


Assuntos
Condrócitos/patologia , MicroRNAs/metabolismo , Osteoartrite , Animais , Apoptose , Modelos Animais de Doenças , Desenvolvimento de Medicamentos , Interleucina-1beta/metabolismo , Janus Quinase 2/metabolismo , Camundongos , Osteoartrite/tratamento farmacológico , Osteoartrite/metabolismo , Estresse Oxidativo , Fator de Transcrição STAT3/metabolismo , Transdução de Sinais
14.
Rev Sci Instrum ; 89(9): 093301, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30278713

RESUMO

In recent experiments at Tsinghua University Accelerator Laboratory, the 31 MeV electron beam, which has been compressed to subpicosecond pulse durations, has been used to generate high peak power, narrow band Terahertz (THz) radiation by transit through different slow wave structures, specifically quartz capillaries metallized on the outside. Despite the high peak powers that have been produced, the THz pulse energy is negligible compared to the energy of the electron beam. Therefore, the THz generation process can be complementary to other beamline applications like plasma wakefield acceleration studies and Compton x-ray free electron lasers. This approach can be used at x-ray free electron laser beamlines, where THz radiation can be generated without disturbing the x-ray generation process. In the experiment reported here, a high peak current electron beam generated strong narrow band (∼1% bandwidth) THz signals in the form of a mixture of TM01 and TM02 modes. Each slow wave structure is completed with a mode converter at the end of the structure that allows for efficient (>90%) power extraction into free space. In the experiment, both modes in these two dielectric-loaded waveguides TM01 (0.3 THz/0.5 THz) and TM02 (0.9 THz/1.3 THz) were explicitly measured with an interferometer. The THz pulse energy was measured with a calibrated Golay cell at a few µJ.

15.
Environ Sci Pollut Res Int ; 25(27): 27196-27202, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30027376

RESUMO

Fe-based metal-organic framework MIL-88A microrods were synthesized by hydrothermal method, which were used to adsorb As(V) in water for the first time. The experimental results indicated that MIL-88A has a very fast adsorption rate towards arsenic in water. The kinetic and isothermal data for arsenic removal were better fitted to the pseudo-second-order kinetic model and Langmuir model, respectively, implying a chemical and monolayer adsorption for As(V) on MIL-88A microrods. Two rate-controlling processes during adsorption were revealed by the intraparticle diffusion model. The maximum adsorption capacity of MIL-88A reached 145 mg g-1, higher than those of Fe-based MIL adsorbents reported previously, which probably originates from its unique microstructure with abundant OH- groups and an unusual large swelling towards water. These show that Fe-based MIL-88A is a good candidate for arsenic removal.


Assuntos
Arsênio/química , Estruturas Metalorgânicas/química , Poluentes Químicos da Água/química , Adsorção , Difusão , Cinética , Purificação da Água/métodos
16.
EMBO Rep ; 19(5)2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29599149

RESUMO

Aggregation of α-synuclein is a hallmark of Parkinson's disease and dementia with Lewy bodies. We here investigate the relationship between cytosolic Ca2+ and α-synuclein aggregation. Analyses of cell lines and primary culture models of α-synuclein cytopathology reveal an early phase with reduced cytosolic Ca2+ levels followed by a later Ca2+ increase. Aggregated but not monomeric α-synuclein binds to and activates SERCA in vitro, and proximity ligation assays confirm this interaction in cells. The SERCA inhibitor cyclopiazonic acid (CPA) normalises both the initial reduction and the later increase in cytosolic Ca2+ CPA protects the cells against α-synuclein-aggregate stress and improves viability in cell models and in Caenorhabditis elegans in vivo Proximity ligation assays also reveal an increased interaction between α-synuclein aggregates and SERCA in human brains affected by dementia with Lewy bodies. We conclude that α-synuclein aggregates bind SERCA and stimulate its activity. Reducing SERCA activity is neuroprotective, indicating that SERCA and down-stream processes may be therapeutic targets for treating α-synucleinopathies.


Assuntos
Cálcio/química , Cálcio/metabolismo , Citosol/química , Agregados Proteicos , ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/metabolismo , alfa-Sinucleína/metabolismo , Animais , Encéfalo/patologia , Caenorhabditis elegans , Linhagem Celular , Células Cultivadas , Retículo Endoplasmático/metabolismo , Humanos , Indóis/farmacologia , Corpos de Lewy , Masculino , Camundongos , Doença de Parkinson/patologia , Ratos , ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/antagonistas & inibidores
17.
Indian J Gastroenterol ; 37(2): 79-85, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29516416

RESUMO

OBJECTIVE: The aim of this study is to evaluate the efficacy of narrow-band imaging (NBI) in the detecting early esophageal cancer and precancerous lesions and to investigate the risk factors for its occurrence. METHODS: The esophagus was examined with ordinary endoscopy, NBI, and iodine staining. All the lesions were confirmed by histopathologically as the gold standard; NBI and intrapapillary capillary scale (IPCL) scale were compared with pathologic diagnosis. The accuracy, sensitivity, specificity, positive and negative predictive values (PPV, NPV) were calculated. Subgroup analysis was performed between the elderly vs. younger group, and head and neck squamous cell cancer (HNSCC) vs. non-HNSCC patients. RESULTS: Ninety lesions were detected with ordinary endoscopy, 108 with NBI, and 120 with iodine staining. All esophageal cancers were detected both by NBI and by iodine staining. Accuracy, sensitivity, and specificity for esophageal cancer and precancerous lesion were 67.8%, 58.1%, and 76.6%; 92%, 89.7%, and 96%; 93.4%, 93.4%, and 93.2%, respectively. NBI endoscopy and iodine staining were superior to ordinary endoscopy for detecting esophageal cancer and precancerous lesions (p < 0.05). NBI showed better detection of esophageal neoplasms in the elderly patients (p < 0.001). The incidence of multiple squamous cell cancers (SCCs) was significantly higher in non-elderly group (p = 0.009). NBI can also detect more esophageal neoplastic lesions in patients with head and neck cancers (p = 0.003). CONCLUSIONS: NBI endoscopy appears as effective as Lugol staining to detect and screen the early esophageal cancer. NBI shows better detection of esophageal neoplasms in the elderly patients. The incidence of multiple SCCs was much higher in non-elderly patients.


Assuntos
Carcinoma de Células Escamosas/diagnóstico por imagem , Carcinoma de Células Escamosas/etiologia , Endoscopia Gastrointestinal , Neoplasias Esofágicas/diagnóstico por imagem , Neoplasias Esofágicas/etiologia , Imagem de Banda Estreita , Fatores Etários , Idoso , Carcinoma de Células Escamosas/epidemiologia , Detecção Precoce de Câncer , Neoplasias Esofágicas/epidemiologia , Feminino , Humanos , Incidência , Iodo , Masculino , Pessoa de Meia-Idade , Lesões Pré-Cancerosas/diagnóstico por imagem , Lesões Pré-Cancerosas/epidemiologia , Lesões Pré-Cancerosas/etiologia , Valor Preditivo dos Testes , Fatores de Risco , Sensibilidade e Especificidade , Coloração e Rotulagem
18.
Sci Rep ; 7(1): 12393, 2017 09 29.
Artigo em Inglês | MEDLINE | ID: mdl-28963542

RESUMO

Nanotwinned copper (nt-Cu) shows a broad application prospects as interconnection materials in integrated circuit industry, since it combines the excellent mechanical and electrical properties. However, the formation and growth behavior of twin lamellae in pulse electrodeposited copper films are not fully understood. In this work, a series of electroplated copper films are prepared by verifying the electroplating parameters and the microstructures are analyzed using scanning electron microscope (SEM) and transmission electron microscope (TEM). The surface morphology indicates strong evidence of stacked twin terraces and layers, suggesting that nanotwins grow up layer by layer. Combined with in situ characterization by SEM and molecular dynamics (MD) simulation, it is proved that the terraces originate from the triple junction of grain boundaries and grow up by extending along the lateral (111) crystal plane. A twin terrace-growing model for nt-Cu is then deduced, which distinguishes from deformation twins or annealed twins. This growth model would be prospective to help obtain high quality of nt-Cu in industry.

19.
Exp Neurol ; 295: 243-255, 2017 09.
Artigo em Inglês | MEDLINE | ID: mdl-28601604

RESUMO

Axonal dystrophy has been described as an early pathological feature of neurodegenerative disorders including Alzheimer's disease and amyotrophic lateral sclerosis. Axonal inclusions have also been reported to occur in several neurodegenerative lysosomal storage disorders including Mucopolysaccharidosis type IIIA (MPS IIIA; Sanfilippo syndrome). This disorder results from a mutation in the gene encoding the lysosomal sulphatase sulphamidase, and as a consequence heparan sulphate accumulates, accompanied by secondarily-stored gangliosides. The precise basis of symptom generation in MPS IIIA has not been elucidated, however axonal dystrophy may conceivably lead to impaired vesicular trafficking, neuronal dysfunction and/or death. We have utilised a faithful murine model of MPS IIIA to determine the spatio-temporal profile of neuronal inclusion formation and determine the effect of restoring normal lysosomal function. Dopaminergic (tyrosine hydroxylase-positive), cholinergic (choline acetyltransferase-positive) and GABAergic (glutamic acid decarboxylase65/67-positive) neurons were found to exhibit axonal dystrophy in MPS IIIA mouse brain. Axonal lesions present by ~seven weeks of age were Rab5-positive but lysosomal integral membrane protein-2 negative, suggesting early endosomal involvement. By 9-12-weeks of age, immunoreactivity for the autophagosome-related proteins LC3 and p62 and the proteasomal subunit 19S was noted in the spheroidal structures, together with wildtype α-synuclein, phosphorylated Thr-181 Tau and amyloid precursor protein, indicative of impaired axonal trafficking. Sulphamidase replacement reduced but did not abrogate the axonal lesions. Therefore, if axonal dystrophy impairs neuronal activity and ultimately, neuronal function, its incomplete resolution warrants further investigation.


Assuntos
Axônios/patologia , Encéfalo/patologia , Mucopolissacaridose III/patologia , Precursor de Proteína beta-Amiloide/metabolismo , Animais , Feminino , Hidrolases/genética , Imuno-Histoquímica , Lisossomos/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Mucopolissacaridose III/diagnóstico por imagem , alfa-Sinucleína/metabolismo , Proteínas tau/metabolismo
20.
J Clin Gastroenterol ; 51(3): 223-227, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27306943

RESUMO

OBJECTIVE: To determine whether endoscopic resection (ER) and minimally invasive esophagectomy (MIE) are safe and effective for treating squamous intraepithelial neoplasia of the esophagus. MATERIALS AND METHODS: This study retrospectively analyzed a total of 99 consecutive patients with pathologically confirmed early esophageal cancer between December 2007 and 2011. ER was performed in 59 patients, whereas MIE was performed in 40 patients. We compared the 2 groups according to R0 resection rates, treatment-related complications, mean hospital stay, local recurrence rates, and 3- and 4-year overall survival. RESULTS: No significant differences were found in the R0 resection rates between ER and MIE (94.9% vs. 97.5%, P>0.05). The occurrence rate of minor complications in the ER group was significantly lower than that in the thoracoscopic esophagectomy group (11.8% vs. 32.5%, P>0.05). The mean operative time in the ER group was 74±23 minutes, which was significantly shorter than that in the MIE group (298±46 min). The average length of hospital stay in the ER group was significantly shorter than that in the MIE group (P<0.001). No significant differences were observed in the local recurrence rates between the 2 groups (P>0.05). Similarly, no differences were found in the 3-year survival rate (ER: 96.6%, vs. MIE: 97.5%, P>0.05) and 4-year survival rate (ER: 91.5% vs. MIE: 90%, P>0.05) between the 2 groups. CONCLUSIONS: ER achieves the same positive results as MIE in the treatment of early esophageal cancer and is associated with a lower complication rate, a shorter recovery time, and a similar survival rate. However, multiple ER procedures were required for several patients in this study.


Assuntos
Carcinoma de Células Escamosas/cirurgia , Neoplasias Esofágicas/cirurgia , Recidiva Local de Neoplasia/cirurgia , Carcinoma de Células Escamosas/mortalidade , Carcinoma de Células Escamosas/patologia , China , Intervalo Livre de Doença , Neoplasias Esofágicas/mortalidade , Neoplasias Esofágicas/patologia , Esofagectomia , Esofagoscopia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Procedimentos Cirúrgicos Minimamente Invasivos , Recidiva Local de Neoplasia/mortalidade , Recidiva Local de Neoplasia/patologia , Estudos Retrospectivos , Análise de Sobrevida , Resultado do Tratamento
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