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1.
Adv Med Sci ; 65(1): 127-133, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31927424

RESUMO

PURPOSE: The aim of this study is to compare levels of nasal nitric oxide (nNO) in pediatric patients with respiratory diseases. MATERIALS AND METHODS: nNO was measured by an electrochemical analyzer in 179 patients aged 7-15 with asthma, allergic rhinitis or with asthma and allergic rhinitis and in healthy children recruited from a local allergology clinic. Correlations between nNO levels and patient clinical parameters were assessed. RESULTS: nNO was significantly higher in patients with allergic rhinitis (2316.3 ± 442.33 ppb, p < 0.001) as well as with asthma and allergic rhinitis (2399.9 ± 446.73 ppb, p < 0.001) compared to asthmatic and healthy children (1066.4 ± 416.75; 836.2 ± 333.47 ppb, respectively). A receiver operating characteristic curve analysis revealed that a cut-off value of 1545 ppb nNO and 1459 ppb nNO has sensitivity of 100% and specificity of 100% in distinguishing allergic rhinitis and combined asthma and allergic rhinitis from healthy subjects. A positive correlation between nNO and age and height was determined only in groups of healthy controls. We found no association between nNO level and clinical parameters including percent of eosinophils and total IgE. CONCLUSION: Levels of nNO are currently measured by different analyzers and with different methods, so assessment of nNO is in need of standardization improvement to become a more reliable tool. However, because it is cheap, painless and fast, it may be helpful in combination with recognition of clinical symptoms and typical diagnostic methods, especially in estimation of inflammation.

2.
J Breath Res ; 13(4): 046007, 2019 07 18.
Artigo em Inglês | MEDLINE | ID: mdl-31234165

RESUMO

Measurement of nitric oxide (NO) levels in exhaled air from the upper and lower airways is currently used as a non-invasive marker of inflammation in respiratory diseases. Assessment of NO exhaled from the lower air respiratory tract is considered to be a quick method for confirmation and control of asthma in patients as well as an estimation of treatment efficiency. The main aim of this study was to determine differences between levels of exhaled nitric oxide (fractional exhaled NO; FeNO) in patients with respiratory disease as measured by an electrochemical analyzer. Measurements were taken in 352 pediatric patients aged 4-17 with cystic fibrosis (CF) (n = 43), asthma (n = 69), allergic rhinitis (AR) (n = 70), asthma and AR (n = 128) and non-diseased children (n = 42) recruited from the Allergology Outpatient Department, Provincial Hospital No 2, Rzeszów. The second objective of this study was to assess any correlations between FeNO and clinical parameters of patients. The level of FeNO in patients with CF was normal when compared with control subjects (10.8 ± 2.9 versus 11.4 ± 6 ppb). We found significantly higher FeNO in patients with asthma (26.6 ± 15.3 ppb, p < 0.001), AR (18.4 ± 9.6 ppb, p < 0.01) as well as in patients with both asthma and AR (43.3 ± 31.1 ppb, p < 0.001) when compared to healthy children. Statistical analysis revealed a positive correlation between FeNO and age, height and weight of control subjects, and height in children with AR. FeNO was independent of sex, BMI, spirometry and blood results as well as the type of residence in control children and subjects with CF, asthma, AR and combined asthma and AR. In conclusion, we found normal levels of FeNO in children with CF and elevated levels in patients with asthma, AR and combined asthma and AR as compared to control subjects. Due to conflicting data, there is still a need for additional research, especially related to regarding factors that affect FeNO levels in respiratory disease.


Assuntos
Testes Respiratórios/métodos , Expiração , Óxido Nítrico/análise , Doenças Respiratórias/diagnóstico , Adolescente , Biomarcadores/sangue , Estudos de Casos e Controles , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Análise Multivariada , Óxido Nítrico/sangue , Análise de Regressão , Doenças Respiratórias/sangue , Caracteres Sexuais
3.
Acta Biochim Pol ; 66(1): 13-21, 2019 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-30816367

RESUMO

Resveratrol is a polyphenol that is abundant in grape skin and seeds. Food sources of resveratrol include wine, berries, and peanuts. This compound has many properties, including activity against glycation, oxidative stress, inflammation, neurodegeneration, several types of cancer, and aging. Because resveratrol is generally  welltolerated, it is believed to be a promising compound in preventing many diseases, such as diabetes and its complications. Unfortunately, this compound exhibits low bioavailability and solubility. The aim of this review is to summarize the latest information on the multiple effects of resveratrol on health and the benefits of its intake, based on in vitro and in vivo studies in animals and humans.


Assuntos
Resveratrol/uso terapêutico , Envelhecimento/efeitos dos fármacos , Animais , Anti-Inflamatórios/uso terapêutico , Humanos , Neoplasias/prevenção & controle , Neuroproteção/efeitos dos fármacos
4.
Oxid Med Cell Longev ; 2017: 7905148, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29181127

RESUMO

Multiple sclerosis (MS) is a disease involving oxidative stress (OS). This study was aimed at examination of the effect of melatonin supplementation on OS parameters, especially oxidative protein modifications of blood serum proteins, in MS patients. The study included 11 control subjects, 14 de novo diagnosed MS patients with the relapsing-remitting form of MS (RRMS), 36 patients with RRMS receiving interferon beta-1b (250 µg every other day), and 25 RRMS patients receiving interferon beta-1b plus melatonin (5 mg daily). The levels of N'-formylkynurenine, kynurenine, dityrosine, carbonyl groups, advanced glycation products (AGEs), advanced oxidation protein products (AOPP), and malondialdehyde were elevated in nontreated RRSM patients. N'-Formylkynurenine, kynurenine, AGEs, and carbonyl contents were decreased only in the group treated with interferon beta plus melatonin, while dityrosine and AOPP contents were decreased both in the group of patients treated with interferon beta and in the group treated with interferon beta-1b plus melatonin. These results demonstrate that melatonin ameliorates OS in MS patients supporting the view that combined administration of interferon beta-1b and melatonin can be more effective in reducing OS in MS patients than interferon beta-1b alone.


Assuntos
Proteínas Sanguíneas/metabolismo , Interferon beta/uso terapêutico , Melatonina/uso terapêutico , Esclerose Múltipla/tratamento farmacológico , Adulto , Feminino , Humanos , Interferon beta/farmacologia , Masculino , Melatonina/farmacologia , Esclerose Múltipla/patologia , Estresse Oxidativo
5.
J Neuroimmunol ; 305: 145-153, 2017 04 15.
Artigo em Inglês | MEDLINE | ID: mdl-28284335

RESUMO

Myasthenia gravis (MG) is an autoimmune disease caused by production of antibodies against acetylcholine receptors of the neuromuscular junction (Ab). The aim of this study was to ascertain if oxidative stress accompanies MG by estimation of the several independent parameters of oxidative damage, mainly the levels of oxidative modifications of blood serum proteins. The group studied consisted of 50 MG patients (28 females and 22 males), 24 with ocular MG (OMG) and 26 with generalized MG (GMG), of mean age of 66.7 (30-81)years (y), mean disease duration of 9.5 (0.5-34)y, mean level of Ab of 8.9 (0.1-85)nmol/ml, and 25 age-matched healthy controls. MG patients were stratified into groups according to disease duration (<5y or >5y), Ab level (low, <3 or high, >3nmol/l) as well as symptoms (GMG or OMG). Glycophore fluorescence was increased in OMGa. Dityrosine was increased in both types of MGc, in patients ill <5b and >5cy, with lowc and highc levels of Ab. N-formylkynurenine was increased in OMGa and GMGb, in both disease duration groupsa, in the group of low Aba. Kynurenine was increased in the group with high Aba. Tryptophan fluorescence was decreased in OMGb and GMGc, in patients ill for <5b and >5ay, with lowa and highc Ab. Serum thiol group concentration were decreased in GMGc, in patients ill for >5yb. AOPP level was elevated in OMGa, in patients ill for >5ya with high Aba. Protein carbonyls were increased in both OMGc and GMGc, in patients ill for >5ay, with lowb and highb Ab. FRAP and ABTS• scavenging by fast antioxidants were unchanged, but ABTS• scavenging by slow antioxidants was lower in OMGb and GMGc, in patients ill for >5cy, in patients with lowc and highb Ab (ap<0.05, bp<0.01, cp<0.001). These results demonstrate systemic oxidative stress in MG, suggesting therapeutic use of antioxidants.


Assuntos
Proteínas Sanguíneas/metabolismo , Miastenia Gravis/sangue , Estresse Oxidativo/fisiologia , Adulto , Produtos da Oxidação Avançada de Proteínas/metabolismo , Idoso , Idoso de 80 Anos ou mais , Autoanticorpos/sangue , Benzotiazóis/metabolismo , Progressão da Doença , Feminino , Glicoforinas/metabolismo , Humanos , Cinurenina/metabolismo , Masculino , Pessoa de Meia-Idade , Miastenia Gravis/cirurgia , Carbonilação Proteica , Receptores Colinérgicos/imunologia , Ácidos Sulfônicos/metabolismo , Timectomia , Tirosina/análogos & derivados , Tirosina/metabolismo
6.
Gen Physiol Biophys ; 36(2): 175-186, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28218612

RESUMO

Glycation is the cause of diabetic complications and contributes to the development of other diseases and aging. Numerous exogenous compounds have been tested for their anti-glycating activity. The aim of this study was to answer the question, which endogenous compounds at physiological concentrations can effectively prevent glycation. A set of endogenous compounds has been tested for the ability to protect albumin from glucose-induced glycation in vitro at a concentration of 1 mM and in a physiological concentration range. Only glutathione was found to protect significantly against glycation at physiological concentrations. Glutathione depletion increased the rate of hemoglobin glycation in erythrocytes incubated with high glucose concentrations. These results indicate that the level of glutathione is the main determinant of glycation of intracellular proteins.


Assuntos
Glucose/química , Glucose/metabolismo , Glutationa/sangue , Glutationa/química , Hemoglobinas/química , Hemoglobinas/metabolismo , Adulto , Proteínas Sanguíneas/química , Proteínas Sanguíneas/metabolismo , Células Cultivadas , Feminino , Produtos Finais de Glicação Avançada/sangue , Produtos Finais de Glicação Avançada/química , Humanos , Soroalbumina Bovina/química
7.
Acta Biochim Pol ; 64(1): 195-198, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-27991936

RESUMO

Deoxyribose test has been widely used for determination of reactivities of various compounds for the hydroxyl radical. The test is based on the formation of hydroxyl radical by Fe2+ complex in the Fenton reaction. We propose a modification of the deoxyribose test to detect strong iron binding, inhibiting participation of Fe2+ in the Fenton reaction, on the basis of examination of concentration dependence of deoxyribose degradation on Fe2+ concentration, at a constant concentration of a chelating agent.


Assuntos
Desoxirribose/química , Peróxido de Hidrogênio/química , Radical Hidroxila/química , Ferro/química , Sítios de Ligação , Quelantes/química , Técnicas de Laboratório Clínico/métodos , Relação Dose-Resposta a Droga , Quelantes de Ferro/química
8.
Schizophr Res ; 176(2-3): 245-251, 2016 10.
Artigo em Inglês | MEDLINE | ID: mdl-27449251

RESUMO

The aim of this study was to compare the antioxidant activities of six atypical antipsychotic drugs: clozapine (CLZ), quetiapine, olanzapine (OLA), risperidone, ziprasidone, aripiprazole (ARI), as well as a typical antipsychotic drug, haloperidol. Several tests of antioxidant activity were used: protection of thiol groups against oxidation by peroxynitrite (PN) and 3-morpholinosydnonimine (SIN-1, generator of PN), oxidation of dihydrorhodamine 123 by PN, SIN-1 and hypochlorite (NaOCl), bleaching of fluorescein fluorescence by PN, 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH, generator of peroxyl radicals) and NaOCl, radical-scavenging activity with respect to 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) radical, 2,2-diphenyl-1-picrylhydrazyl free radical and the Ferric Reducing Antioxidant Potential. In most of the tests, OLA showed the highest antioxidant activity, followed by CLZ and in some cases ARI, other compounds being much less active or not active. OLA and CLZ exerted limited toxicity on mouse neuroblastoma Neuro-2A (N2A) cells and protected the cells against the toxic action of SIN-1, AAPH and NaOCl in the physiologically relevant concentration range of these oxidants. Both drugs reduced the PN-induced nitration of intracellular proteins. Given that schizophrenia is associated with oxidative and nitrosative stress, the direct antioxidant activity OLA and CLZ may contribute to the therapeutic action of these compounds.


Assuntos
Antioxidantes/farmacologia , Antipsicóticos/farmacologia , Esquizofrenia/tratamento farmacológico , Animais , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Camundongos , Estresse Oxidativo/efeitos dos fármacos
9.
Cell Mol Biol Lett ; 20(4): 562-70, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26146126

RESUMO

The role of metal (especially) iron ions has been postulated to play a prominent role in protein glycation, suggesting antiglycating effectiveness of metal chelators. However, this rule may not apply to all model glycation systems. We found that metal chelators are not effective in prevention of glycation of bovine serum albumin (BSA) in vitro, and there is no correlation between the antiglycating effects of 32 compounds and their iron chelation activity as measured with the ferrozine test. These data indicate that the glycation of BSA in vitro is iron-independent and is not a proper system to study the role of metals in protein glycation.


Assuntos
Quelantes/química , Produtos Finais de Glicação Avançada , Soroalbumina Bovina/química , Catalase/química , Quelantes/farmacologia , Ferrozina/química , Ferrozina/metabolismo , Glioxal/química , Glioxal/metabolismo , Técnicas In Vitro , Concentração Inibidora 50 , Ferro/química , Aldeído Pirúvico/química , Aldeído Pirúvico/metabolismo , Resinas Sintéticas/química , Soroalbumina Bovina/metabolismo , Superóxido Dismutase/química
10.
Redox Biol ; 5: 381-387, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26141922

RESUMO

Exposure to high glucose concentrations in vitro is often employed as a model for understanding erythrocyte modifications in diabetes. However, effects of such experiments may be affected by glucose consumption during prolonged incubation and changes of cellular parameters conditioned by impaired energy balance. The aim of this study was to compare alterations in various red cell parameters in this type of experiment to differentiate between those affected by glycoxidation and those affected by energy imbalance. Erythrocytes were incubated with 5, 45 or 100mM glucose for up to 72 h. High glucose concentrations intensified lipid peroxidation and loss of activities of erythrocyte enzymes (glutathione S-transferase and glutathione reductase). On the other hand, hemolysis, eryptosis, calcium accumulation, loss of glutathione and increase in the GSSG/GSH ratio were attenuated by high glucose apparently due to maintenance of energy supply to the cells. Loss of plasma membrane Ca(2+)-ATPase activity and decrease in superoxide production were not affected by glucose concentration, being seemingly determined by processes independent of both glycoxidation and energy depletion. These results point to the necessity of careful interpretation of data obtained in experiments, in which erythrocytes are subject to treatment with high glucose concentrations in vitro.


Assuntos
Eritrócitos/efeitos dos fármacos , Glucose/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , ATPases Transportadoras de Cálcio/metabolismo , Catalase/metabolismo , Eritrócitos/citologia , Eritrócitos/metabolismo , Glutationa/metabolismo , Glutationa Redutase/metabolismo , Glutationa Transferase/metabolismo , Hemoglobina A Glicada/análise , Hemólise/efeitos dos fármacos , Humanos , Superóxidos/análise
11.
Redox Biol ; 6: 93-99, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26202868

RESUMO

Ascorbic acid (AA) has been reported to be both pro-and antiglycating agent. In vitro, mainly proglycating effects of AA have been observed. We studied the glycation of bovine serum albumin (BSA) induced by AA in vitro. BSA glycation was accompanied by oxidative modifications, in agreement with the idea of glycoxidation. Glycation was inhibited by antioxidants including polyphenols and accelerated by 2,​2'-​azobis-​2-​methyl-​propanimidamide and superoxide dismutase. Nitroxides, known to oxidize AA, did not inhibit BSA glycation. A good correlation was observed between the steady-state level of the ascorbyl radical in BSA samples incubated with AA and additives and the extent of glycation. On this basis we propose that ascorbyl radical, in addition to further products of AA oxidation, may initiate protein glycation.


Assuntos
Ácido Ascórbico/química , Produtos Finais de Glicação Avançada/química , Soroalbumina Bovina/química , Amidinas/química , Animais , Antioxidantes/química , Bovinos , Quelantes/química , Espectroscopia de Ressonância de Spin Eletrônica , Radicais Livres , Glicosilação , Oxidantes/química , Oxirredução , Polifenóis/química , Soluções , Espectrometria de Fluorescência , Superóxido Dismutase/química
12.
Molecules ; 19(11): 18828-49, 2014 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-25407721

RESUMO

The aim of this study was to compare the kinetics of the glycoxidation of bovine serum albumin (BSA) as a model protein by three sugars: glucose, fructose and ribose, using fluorometric measurements of the content of advanced glycation end products (AGEs), protein-bound fructosamine, dityrosine, N'-formylkynurenine, kynurenine, tryptophan, the content of advanced oxidation protein products (AOPP), protein carbonyl groups, as well as thiol groups. Moreover, the levels of glycoalbumin and AGEs were determined by using an enzyme-linked immunosorbent assay. Based on the kinetic results, the optimal incubation time for studies of the modification of the glycoxidation rate by additives was chosen, and the effects of 25 compounds of natural origin on the glycoxidation of BSA induced by various sugars were examined. The same compounds were found to have different effects on glycoxidation induced by various sugars, which suggests caution in extrapolation from experiments based on one sugar to other sugars. From among the compounds tested, the most effective inhibitors of glycoxidation were: polyphenols, pyridoxine and 1-cyano-4-hydroxycinnamic acid.


Assuntos
Frutose/química , Glucose/química , Ribose/química , Soroalbumina Bovina/química , Produtos da Oxidação Avançada de Proteínas/química , Animais , Bovinos , Ácidos Cumáricos/química , Frutosamina/química , Produtos Finais de Glicação Avançada/química , Cinética , Cinurenina/análogos & derivados , Cinurenina/química , Polifenóis/química , Piridoxina/química , Albumina Sérica/química , Triptofano/química , Tirosina/análogos & derivados , Tirosina/química
13.
Oxid Med Cell Longev ; 2014: 389629, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24803981

RESUMO

Pseudomonas aeruginosa and Staphylococcus aureus cause chronic lung infection in cystic fibrosis (CF) patients, inducing chronic oxidative stress. Several markers of plasma protein oxidative damage and glycoxidation and activities of erythrocyte antioxidant enzymes have been compared in stable CF patients chronically infected with Pseudomonas aeruginosa (n = 12) and Staphylococcus aureus (n = 10) in relation to healthy subjects (n = 11). Concentration of nitric oxide was also measured in the exhaled air from the lower respiratory tract of patients with CF. Elevated glycophore (4.22 ± 0.91 and 4.19 ± 1.04 versus control 3.18 ± 0.53 fluorescence units (FU)/mg protein; P < 0.05) and carbonyl group levels (1.9 ± 0.64, 1.87 ± 0.45 versus control 0.94 ± 0.19 nmol/mg protein; P < 0.05) as well as increased glutathione S-transferase activity (2.51 ± 0.88 and 2.57 ± 0.79 U/g Hb versus 0.77 ± 0.16 U/g Hb; P < 0.05) were noted in Pseudomonas aeruginosa and Staphylococcus aureus infected CF. Kynurenine level (4.91 ± 1.22 versus 3.89 ± 0.54 FU/mg protein; P < 0.05) was elevated only in Staphylococcus aureus infected CF. These results confirm oxidative stress in CF and demonstrate the usefulness of the glycophore level and protein carbonyl groups as markers of oxidative modifications of plasma proteins in this disease.


Assuntos
Infecções Bacterianas/diagnóstico , Proteínas Sanguíneas/metabolismo , Fibrose Cística/diagnóstico , Pseudomonas aeruginosa/patogenicidade , Staphylococcus aureus/patogenicidade , Adolescente , Infecções Bacterianas/complicações , Infecções Bacterianas/microbiologia , Proteínas Sanguíneas/química , Criança , Fibrose Cística/complicações , Fibrose Cística/metabolismo , Eritrócitos/citologia , Eritrócitos/enzimologia , Feminino , Glutationa Transferase/metabolismo , Humanos , Cinurenina/metabolismo , Masculino , Óxido Nítrico/metabolismo , Estresse Oxidativo , Carbonilação Proteica , Sistema Respiratório/enzimologia , Sistema Respiratório/metabolismo , Sistema Respiratório/microbiologia
14.
Molecules ; 19(4): 4880-96, 2014 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-24747646

RESUMO

The aim of this study was to compare several methods for measurement of bovine serum albumin (BSA) modification by glycoxidation with reactive dicarbonyl compounds (methylglyoxal--MGO and glyoxal--GO), for studies of the kinetics of this process and to compare the effects of 19 selected compounds on BSA glycation by the aldehydes. The results confirm the higher reactivity of MGO with respect to GO and point to the usefulness of AGE, dityrosine and N'-formylkynurenine fluorescence for monitoring glycation and evaluation of protection against glycation. Different extent of protection against glycation induced by MGO and GO was found for many compounds, probably reflecting effects on various stages of the glycation process. Polyphenols (genistein, naringin and ellagic acid) were found to protect against aldehyde-induced glycation; 1-cyano-4-hydroxycinnamic acid was also an effective protector.


Assuntos
Glioxal/química , Aldeído Pirúvico/química , Soroalbumina Bovina/química , Animais , Bovinos , Ácidos Cumáricos/química , Ácido Elágico/química , Flavanonas/química , Genisteína/química , Produtos Finais de Glicação Avançada/química , Glicosilação , Glioxal/antagonistas & inibidores , Cinética , Cinurenina/análogos & derivados , Cinurenina/química , Oxirredução , Aldeído Pirúvico/antagonistas & inibidores , Espectrometria de Fluorescência
15.
Free Radic Biol Med ; 75 Suppl 1: S47, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26461390

RESUMO

Glycoxidation belongs to posttranslational protein modifications which underlie pathological sequelae of diabetes and other diseases, and contribute to aging. Search for efficient inhibitors of glycoxidation is therefore of considerable importance. We studied the effect of various polyphenols on the glycoxidation of bovine serum albumin (90 uM) incubated in vitro with glucose, fructose or ribose (100mM) for 6 days in 0.1M phosphate buffer, pH 7.4. Polyphenols have multiple biological actions including antioxidant activity and chelation of transition metal ions. The extent of glycoxidation was evaluated using fluorimetic parameters reflecting formation of Advanced Glycoxidation End Products (AGEs: 325/440nm), dityrosine (330/415nm), formylkynurenine (325/434nm) and kynurenine (365/480nm) and confirmed by estimation of AGEs using an ELISA kit. The results confirmed reliability of easily measurable fluorimetric parameters such as AGEs, dityrosine and formylkynurenine level for estimation of the extent of glycoxidation.All the polyphenols used (caffeic acid, ferulic acid, gallic acid, genistein, naringin, propyl gallate, quercitrin and rutin) decreased the extent of albumin glycoxidation. The extent of protection varied for different sugars (e. g. 1mM genistein: 24.4±1.7 for glucose, 44.5±0.2 for fructose 51.4±0.3 for ribose) The sequence of protective effect was: ferulic acid>caffeic acid>propyl gallate>naringin>quercitrin>genistein for glucose, caffeic acid>ferulic acid>propyl gallate>genistein>quercitrin>rutin>naringin for fructose and genistein>ferulic acid>caffeic acid>rutin>propyl gallate>naringin>quercitrin>gallic acid. These results confirm that polyphenols, natural components of human diet, protect against protein glycation in a model in vitro system. This study was performed within the framework of COSTCM1001 action and was sponsored by Grant 2011/01/M/N23-02065 of the National Science Center of Poland.

16.
Neurochem Int ; 63(5): 507-16, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-24036284

RESUMO

Multiple sclerosis (MS) has been demonstrated to involve oxidative stress and augmented glycoxidation. In this study, several markers of protein oxidative damage and glycoxidation have been compared in 14 relapsing remittent in MS (RRMS) patients without immunomodifying treatment, 10 patients in clinical relapse, and clinically stable patient groups treated with interferon ß 1a (18) , ß 1b (19) and glatiramer acetate (GA; 6) in relation to healthy subjects (12). The glycophore content was increased in RRSM patients without treatment and in patients treated with GA. The level of advanced protein oxidation products (AOPP) was increased in RRSM patients without treatment and in patients with clinical relapse. The level of protein carbonyls was elevated in RRSM patients without treatment and in patients treated with interferon ß 1b. The levels of dityrosine level and N'-formylkynureine were elevated in RRSM patients without treatment while serum protein thiol groups were decreased in RRSM patients in clinical relapse as well as RRMS patients treated with interferon ß 1a. Several markers of protein modification showed correlation with the C-reactive protein level and white blood cell count, suggesting that oxidative protein modifications are linked to the inflammatory processes in MS. Results of this study confirm the occurrence of protein oxidative and glycoxidative damage in MS and show that spectrophotometric and fluorimetric markers of this damage, especially the AOPP level, may be useful in monitoring oxidative stress in the course of therapy of MS.


Assuntos
Proteínas Sanguíneas/metabolismo , Esclerose Múltipla/sangue , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Oxirredução
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