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1.
J Asian Nat Prod Res ; : 1-6, 2019 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-31829036

RESUMO

Two new 1,3-benzodioxole derivatives, leucandioxoles A and B (1-2), together with two known related compounds (3-4), have been isolated from the South China Sea sponge Leucandra sp. The structures of all compounds were clearly elucidated on the basis of spectroscopic analyses and compared with the literatures. The cytotoxicity against A549, Hep G2, MDA-MB-231, and HeLa cell lines of 1-4 were evaluated. Only compound 1 exhibited moderate activity against MDA-MB-231 cells with the IC50 value of 7.98 ± 0.74 µM.

2.
Artigo em Inglês | MEDLINE | ID: mdl-30818215

RESUMO

Surimi products have become increasingly-consumed food with prominent characteristics of high nutrition and convenience and its supply falls short of demand. However, due to exhausted fishery resource in recent years, surimi adulteration, such as addition of plant proteins, starch and other animal origin meat, is becoming serious, so recognition of these exogenous substances has become an urgent issue. In this study, Fourier transform infrared spectroscopy (FT-IR) combined with infrared spectroscopic imaging could distinguish heterogeneity in surimi qualitatively and quantitatively and obtain integral chemical images so that spatial distribution of each component in surimi could be visually displayed, thus a rapid recognition method and a prediction model were developed. The different starch contents in surimi had been primarily identified through intensity change of infrared absorption peaks at 1045cm-1 and 988cm-1, specifically with peak shifts to 1041cm-1 and to 992cm-1, respectively. In infrared imaging analysis, principal components (PCs) were separated and one key PC was confirmed as starch by characteristic peaks comparison at 1147cm-1, 1075cm-1, 997cm-1 and 930cm-1. Meanwhile, an established statistic model could predict starch content in surimi correctly with a reliable correlation coefficient (R=0.9856) and root mean square error of prediction (RMSEP=5.64). Therefore, FT-IR combined with infrared spectroscopic imaging could be applicable to integrally recognize and quantitatively detect starch in surimi.


Assuntos
Produtos Pesqueiros/análise , Espectrofotometria Infravermelho/métodos , Amido/análise , Análise de Componente Principal , Sensibilidade e Especificidade , Amido/química
3.
Food Chem ; 229: 458-463, 2017 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-28372201

RESUMO

Formaldehyde abuse for retaining squid freshness is detrimental to public health. The aim is to establish a rapid and quantitative detection method of formaldehyde in squid for screening massive samples. A linear relationship between formaldehyde concentration in squid and formaldehyde concentration in squid soaked water was observed using HPLC. Chemical profile variances of squids were rapidly analyzed by Tri-step infrared spectroscopy. Specifically, with increasing formaldehyde concentration, peak intensities of 2927cm-1 (vas(CH2)), 1081cm-1 (glycogen), 1631cm-1 (ß-sheet proteins) decreased while 1655cm-1 (α-helix proteins) increased. Spectral curve-fitting results further disclosed that ß-sheet proteins were transformed to α-helix and ß-turn conformations. Furthermore, a quantitative prediction model based on IR spectra was established by PLS (R2, 0.9787; RMSEC, 5.51). The developed method was applicable for rapid (c.a. 5min) and quantitative analysis of formaldehyde in squids with LOD of 15mg/kg.


Assuntos
Decapodiformes/química , Contaminação de Alimentos/análise , Formaldeído/análise , Espectrofotometria Infravermelho/métodos , Animais , Análise dos Mínimos Quadrados , Limite de Detecção
4.
J Asian Nat Prod Res ; 17(12): 1231-8, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26699877

RESUMO

Three new aaptamine derivatives (1-3), together with six known related compounds (4-9), have been isolated from the South China Sea sponge Aaptos aaptos. The structures of all compounds were unambiguously elucidated on the basis of spectroscopic analyses. Compounds 1, 4, 5, 7, and 9 showed cytotoxic activities against HeLa, K562, MCF-7, and U937 cell lines with IC50 values in the range of 0.90-12.32 µM.


Assuntos
Antineoplásicos/isolamento & purificação , Naftiridinas/isolamento & purificação , Poríferos/química , Animais , Antineoplásicos/química , Antineoplásicos/farmacologia , China , Ensaios de Seleção de Medicamentos Antitumorais , Células HeLa , Humanos , Células K562 , Biologia Marinha , Estrutura Molecular , Naftiridinas/química , Naftiridinas/farmacologia , Células U937
5.
J Nat Prod ; 78(5): 1169-73, 2015 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-25932671

RESUMO

Four new macrocyclic lactones, bryostatin 21 (1) and 9-O-methylbryostatins 4, 16, and 17 (2-4), together with three known related compounds, bryostatins 4, 16, and 17 (5-7), have been isolated from an extract of the South China Sea bryozoan Bugula neritina. The structures of all compounds were unambiguously elucidated using detailed spectroscopic analysis. Structurally, the presence of a single methyl group at C-18 in compound 1 has not been observed before for known bryostatins. The isolated macrolides exhibited inhibitory effects against a small panel of human cancer cell lines.


Assuntos
Antineoplásicos/isolamento & purificação , Antineoplásicos/farmacologia , Briostatinas/isolamento & purificação , Briostatinas/farmacologia , Briozoários/química , Macrolídeos/isolamento & purificação , Animais , Antineoplásicos/química , Briostatinas/química , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Macrolídeos/química , Macrolídeos/farmacologia , Estrutura Molecular , Oceanos e Mares
6.
Mar Drugs ; 12(12): 6003-13, 2014 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-25532563

RESUMO

Five new alkaloids of aaptamine family, compounds (1-5) and three known derivatives (6-8), have been isolated from the South China Sea sponge Aaptos aaptos. The structures of all compounds were unambiguously elucidated by spectroscopic analyses, as well as by comparison with the literature data. Compounds 1-2 are characterized with triazapyrene lactam skeleton, whereas compounds 4-5 share an imidazole-fused aaptamine moiety. These compounds were evaluated in antifungal and anti-HIV-1 assays. Compounds 3, 7, and 8 showed antifungal activity against six fungi, with MIC values in the range of 4 to 64 µg/mL. Compounds 7-8 exhibited anti-HIV-1 activity, with inhibitory rates of 88.0% and 72.3%, respectively, at a concentration of 10 µM.


Assuntos
Fármacos Anti-HIV/química , Fármacos Anti-HIV/farmacologia , Antifúngicos/química , Antifúngicos/farmacologia , Naftiridinas/química , Naftiridinas/farmacologia , Poríferos/química , Alcaloides/química , Alcaloides/farmacologia , Animais , China , Fungos/efeitos dos fármacos , HIV-1/efeitos dos fármacos
7.
J Nat Prod ; 77(9): 2124-9, 2014 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-25211032

RESUMO

Nine new aaptamine derivatives (1-9), together with three known related compounds (10-12), have been isolated from the South China Sea sponge Aaptos aaptos. The structures of all compounds were unambiguously elucidated on the basis of spectroscopic analyses. Structurally, compound 1 possesses a piperidinyl group fused to a demethyl(oxy)aaptamine moiety, whereas compounds 3-6 share an imidazole-fused 1H-benzo[de][1,6]naphthyridin-2(4H)-one skeleton. The cytotoxic activities of the compounds were evaluated against various human cancer cell lines, and compounds 2, 8, 11, and 12 showed potent cytotoxicities against HL60, K562, MCF-7, KB, HepG2, and HT-29 cells, with IC50 values in the range of 0.03 to 8.5 µM.


Assuntos
Antineoplásicos/isolamento & purificação , Antineoplásicos/farmacologia , Naftiridinas/isolamento & purificação , Naftiridinas/farmacologia , Poríferos/química , Animais , Antineoplásicos/química , China , Ensaios de Seleção de Medicamentos Antitumorais , Células HL-60 , Células HT29 , Humanos , Concentração Inibidora 50 , Células K562 , Estrutura Molecular , Naftiridinas/química , Ressonância Magnética Nuclear Biomolecular , Oceanos e Mares
8.
Nat Prod Commun ; 9(6): 763-4, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25115073

RESUMO

A new scalarane sesterterpene, sesterstamide (1), together with four known sesterterpenes (2-5), were isolated from the Paracel Islands marine sponge Hyrtios sp. The chemical structures were established on the basis of spectroscopic analysis and comparison with known compounds. The cytotoxic and antileishmanial activities of the isolated compounds were also evaluated.


Assuntos
Poríferos/metabolismo , Sesterterpenos/química , Sesterterpenos/farmacologia , Animais , Antineoplásicos/química , Antineoplásicos/farmacologia , Antiprotozoários/química , Antiprotozoários/farmacologia , Ilhas , Estrutura Molecular , Oceano Pacífico , Poríferos/química
9.
Nat Prod Commun ; 8(12): 1713-4, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24555279

RESUMO

A new bisuracil analogue, 3,3-bis(uracil-1-yl)-propan-1-aminium (1), together with four known N-containing metabolites (2-5), were isolated from the South China Sea sponge Agelas clathrodes. Their chemical structures were established on the basis of spectroscopic and spectrometric analysis and comparison with known compounds. Compound 1 is an unusual naturally occurring bisuracil analogue, and compound 2 was isolated from a natural source for the first time. Compounds 2 and 4 exhibit moderate cytotoxicity against cancer cell line SGC7901.


Assuntos
Poríferos/química , Uracila/análogos & derivados , Animais , Antineoplásicos/isolamento & purificação , Linhagem Celular Tumoral , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Estrutura Molecular , Poríferos/metabolismo
10.
Mar Drugs ; 10(5): 1027-36, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22822354

RESUMO

Three new polyketides, woodylides A-C (1-3), were isolated from the ethanol extract of the South China Sea sponge Plakortis simplex. The structures were elucidated by spectroscopic data (IR, 1D and 2D NMR, and HRESIMS). The absolute configurations at C-3 of 1 and 3 were determined by the modified Mosher's method. Antifungal, cytotoxic, and PTP1B inhibitory activities of these polyketides were evaluated. Compounds 1 and 3 showed antifungal activity against fungi Cryptococcus neoformans with IC50 values of 3.67 and 10.85 µg/mL, respectively. In the cytotoxicity test, compound 1 exhibited a moderate effect against the HeLa cell line with an IC50 value of 11.2 µg/mL, and compound 3 showed cytotoxic activity against the HCT-116 human colon tumor cell line and PTP1B inhibitory activity with IC50 values of 9.4 and 4.7 µg/mL, respectively.


Assuntos
Produtos Biológicos/química , Produtos Biológicos/isolamento & purificação , Plakortis/química , Policetídeos/química , Policetídeos/isolamento & purificação , Animais , Antifúngicos/química , Antifúngicos/isolamento & purificação , Antifúngicos/farmacologia , Organismos Aquáticos/química , Produtos Biológicos/farmacologia , Linhagem Celular Tumoral , China , Cryptococcus neoformans/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais/métodos , Células HCT116 , Células HeLa , Humanos , Concentração Inibidora 50 , Espectroscopia de Ressonância Magnética/métodos , Policetídeos/farmacologia , Água do Mar
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