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1.
Sci Total Environ ; : 134861, 2019 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-31836220

RESUMO

Understanding the influence factors of nitrogen (N) use efficiencies (NUEs) in different stages of the food system at the provincial scale is critical to achieving cleaner food production while ensuring food security. Nevertheless, they are not well understood. Here we comprehensively analyzed NUE and its influence factors at different stages of the provincial food system. The results showed that per unit agricultural land N input increased by 5-92% in 27 provinces, during 1990-2010, resulting in a low NUE for the crop system when N input per unit agricultural land exceeded about 400 kg N ha-1. This situation has brought some positive changes, as N input decreased by 3-271 kg N ha-1 in 77% of the provinces in 2017, relative to that of 2010, but 10 provinces were still over 450 kg N ha-1 in 2017. Animal food production is expected to continue to expand because 35% and 68% of provinces' urban and rural households, respectively, were still below the recommended minimum animal food N consumption recommendation in 2017, posing great challenges for reducing environmental N pollution. An exciting result is that the NUE of the animal system can be improved by increasing the share of animal food contributed by poultry, eggs, milk and fish, to align with the diets recommended by the Chinese Nutrition Society. NUEs of the provincial food systems excluding Inner Mongolia, Xinjiang, Qinghai and Tibet, would increase by 13% if the net imported food N increased by 1 kg capita-1. Nevertheless, virtual NUE-including N input for imported food in the calculation of NUE-should be considered for accurate comparison of the NUEs of the provincial food systems, especially in highly urbanized areas, while N input for non-food animals should be excluded for accurate evaluation of the NUE in pastoral areas, considering their special production systems and feeding structures.

2.
Onco Targets Ther ; 12: 10323-10334, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31819516

RESUMO

Purpose: Long non-coding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) has been reported to dysregulate in many tumors. However, the mechanism of HOTAIR was rarely reported in GC. Methods: The levels of HOTAIR, microRNA-618 (miR-618) and Krueppel-like factor 12 (KLF12) in GC tissues and cells were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The cell viability and apoptotic rate were assessed via cell counting kit-8 (CCK-8) assay and flow cytometry, respectively. The migrating and invading abilities were tested by Transwell assay. The protein levels of KLF12, p-PI3K, PI3K, p-ATK and ATK were measured by Western blot assay. These interactions between miR-618 and HOTAIR or KLF12 were predicted by DIANA tools, and then, dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were conducted to validate these interactions. Besides, the xenograft tumor experiment was performed to further verify the roles of HOTAIR in GC. Results: The levels of HOTAIR and KLF12 were significantly upregulated and the level of miR-618 was strikingly downregulated in GC tissues and cells. miR-618 was verified as a direct target of HOTAIR or KLF12. HOTAIR silencing blocked GC progression and PI3K/ATK signaling pathway by sponging miR-618 and also restrained xenograft tumor growth in vivo. miR-618 inhibited GC progression and PI3K/ATK signaling pathway by targeting KLF12. Mechanistically, HOTAIR modulated KLF12 expression by sponging miR-618 in GC cells. Conclusion: These data unraveled that HOTAIR promoted GC progression through PI3K/ATK signaling pathway via miR-618/KLF12 axis.

3.
Huan Jing Ke Xue ; 40(8): 3501-3508, 2019 Aug 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854755

RESUMO

The external pollution of Dianchi Lake has been effectively controlled with the implementation of the integrated water environment control project. However, further attention should be paid to endogenous pollutants, such as surface sediments. To investigate the distribution of 16 polycyclic aromatic hydrocarbons (PAHs) in surface sediments of Dianchi Lake, PAH concentrations in 19 surface sediment samples (collected in December 2016) were quantitatively measured by gas chromatography-mass spectrometry (GC-MS). The spatial and temporal distribution, sources, and ecological risks were also analyzed. The concentration of total PAHs (TPAHs) in the Dianchi Lake surface sediments varied in the range of 92.31-1546.78 ng·g-1 with an average of 496.30 ng·g-1. The average concentration of TPAHs in the surface sediments from Caohai (932.37 ng·g-1) was much greater than that from Waihai (380.02 ng·g-1). With the implement of the integrated water environment control project, the concentration of TPAHs in the surface sediments from Dianchi Lake was significantly lower than those detected in 2012, and was already relatively low level among other key waterbodies in China. The PAH with the highest concentration was fluoranthene (80.65 ng·g-1) and the substance with the highest toxic equivalent quantity (TEQ) was dibenz[a, h] anthracene (42.97 ng·g-1). The PAHs were mainly composed of 4 ring and 5-6 ring compounds (with the concentration ratio of 40.38% and 40.22%, respectively), which indicated that the proportions of middle-ring and high-ring compounds were generally consistent. The results of the molecular diagnostic ratio analysis showed that the primary source of PAHs in Dianchi Lake surface sediments are biomass and coal combustion. Based on the potential ecological risk marker comparison method, the entire lake was classified as having a low ecological risk, while the ecological risk of Caohai was relatively higher, which should be concerned further. The results provide initial data and act as an important reference for the conservation and improvement of water quality in Dianchi Lake.

4.
Oxid Med Cell Longev ; 2019: 9307256, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31772715

RESUMO

Crystal-cell interactions are a vital step toward kidney stone formation. However, its mechanisms remained unclear. Here, a protein-protein interaction (PPI) network analysis of a kidney stone revealed that the proteins were enriched in a posttranslational protein modification process in the endoplasmic reticulum (ER). The in vitro study showed that the markers of ER stress, including Bip and CHOP, were upregulated, PERK and ATF6 were activated, and XBP-1 mRNA was spliced. An ER stress-specific protein, caspase-12, was activated in the apoptotic cells induced by calcium oxalate monohydrate (COM) crystals. The treatment with tunicamycin, an ER stress inducer, promoted the crystal-cell adhesion assayed by atomic absorption, reduced cell viability assayed by MTT, and downregulated the expression of proteins involved in the crystal formations. The treatment with salubrinal, an ER stress inhibitor, reversed the above effects for both tunicamycin and COM crystals. The aforementioned main observations were supported by in vivo study. These data demonstrated that ER stress was an essentially biological process of crystal-cell interactions. Our findings suggest that blocking ER stress may become a potential approach to preventing a kidney stone.

5.
Kidney Blood Press Res ; 44(6): 1392-1403, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31639794

RESUMO

BACKGROUND AND OBJECTIVES: Vitamin K (VK) plays a major role in modifying the binding of calcium in bones and blood vessels. Understanding the effect of VK on crystal formation in the kidney would contribute to advancing the treatment and prevention of kidney stones. METHODS: Rats were treated with vitamin K1 (VK1) for 8 weeks. VK1 levels were detected and crystal formation were observed. HK2 cells were exposed to calcium oxalate monohydrate crystals. Apoptosis and cell viability were detected. Crystal deposition was analyzed using atomic absorption assay. The adenovirus vectors expressing matrix Gla protein (MGP) and siMGP were constructed to elucidate the effect and mechanism of VK1 on crystal formation. MGP expression in vivo and in vitro was analyzed by Western blot. The mRNA levels of monocyte chemoattractant protein-1 (MCP-1) and collagen I was measured by semiquantitative RT-PCR. RESULTS: The concentrations of VK1 in whole blood and kidney tissues rose under treatment with VK1. Crystal formation was inhibited from the second to the 6th week, the frequency and quality of crystal formation decreased significantly, and the location of crystal formation was limited to a greater extent in the rats treated by VK1 compared to the control group. Warfarin treatment in the crystals-exposed HK2 cells significantly increased the number of crystals adhering to cells and the number of apoptotic cells and reduced cell viability. VK1 treatment reversed warfarin's above influence. VK1 inhibited the upregulations of MCP-1 and collagen I in kidney tissues under crystal load. VK1 treatment increased MGP expression in vivo and in vitro, and MGP is necessary for VK1 to play a role in crystal deposition in cells. CONCLUSIONS: VK1 treatment can inhibit the formation of renal crystals in vivo. VK1 increases MGP expression and functions through MGP to reduce crystal deposition in cells and provide cell protection. Our findings suggest that VK1 treatment could be a potential strategy for the treatment and prevention of nephrolithiasis.

6.
Opt Express ; 27(18): 25902-25911, 2019 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-31510452

RESUMO

A tunable metamaterial absorber is proposed in the terahertz regime. The amplitude and center frequency of the absorber can be tuned independently. Owing to the effective combination of graphene and strontium titanate (STO) in one metamaterial structure, the tunable properties of the amplitude and center frequency are implemented. The amplitude can be tuned by adjusting the chemical potential of graphene sheet, and center frequency can get a shift through temperature changes in the STO material. In a full-wave numerical simulation, the amplitude of the absorber can be tuned from approximately 100% to 35% with a fixed center frequency when chemical potential varies from 0.7 eV to 0.0 eV. The center frequency of the absorber can shift from 0.43 THz to 0.3 THz when temperature changes from 400 K to 200 K. The complex surface impedance of the graphene and permittivity of STO material in this research range are thoroughly examined, and the independently tunable mechanism of the absorber is explored by elucidating the electric field distribution. The influence of the oblique incidence of electromagnetic wave to the absorber is studied. The absorber can be scalable to the infrared and visible frequencies and demonstrates promising application on tunable sensors, filters, and photovoltaic devices.

7.
Med Sci Monit ; 25: 6812-6819, 2019 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-31506418

RESUMO

BACKGROUND Human chronic myelogenous leukemia (CML) is a hematopoietic stem cell disorder with high malignant and invasive activity. lncRNA SNHG5 has been reported to be upregulated in CML. However, whether it affects the proliferation, differentiation, and apoptosis in CML cells is still unknown. This study investigated the role of SNHG5 in CML and revealed the potential mechanism. MATERIAL AND METHODS K562 cells were transfected with shRNA, and the expression level of SNHG5 was assessd by quantitative RT-PCR. The proliferation ability was determined by CCK-8 assay. Western blot analysis was performed to detect protein expressions related to cell proliferation, differentiation, and apoptosis. Cell apoptosis rate was analyzed by flow cytometry. The DNA methylation level was determined by methylation-specific PCR (MSP). RESULTS Our results show that inhibition of SNHG5 induced by RNA interference significantly inhibits K562 cells proliferation and induces cell differentiation with the increased expression of CD42b, CD11b, CD14, GATA-1, and ß-globin. Flow cytometry analysis indicated that inhibition of SNHG5 significantly induced cell apoptosis with decreased expression of Bcl-2 and increased expression of Bax and cleaved capase-3. Additionally, Western blot and MSP analyses confirmed that inhibition of SNHG5 increased the expression of DR4 gene through suppressing its methylation. CONCLUSIONS Inhibition of SNHG5 suppressed K562 cell proliferation through inducing the differentiation and apoptosis by inhibiting methylation of DR4. Therefore, downregulated SNHG5 could play a key role in CML progression, and might provide a new strategy for the treatment of CML.

8.
Oncol Lett ; 18(3): 3381-3387, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31452818

RESUMO

Increasing evidence has demonstrated that circulating microRNAs (miRNAs) can be utilized as potential biomarkers for the diagnosis of cancer, as well as a prognostic tool for the management of the disease. Therefore, the present study aimed to evaluate the predictive ability of miRNA (miR)-155, miR-96 and miR-99a for the diagnosis and prognosis of hepatocellular carcinoma (HCC). Tissues were collected from 30 patients with HCC and their matched adjacent normal liver tissues, as well as from serum samples from 30 patients with HCC and 30 healthy controls. Reverse transcription-quantitative PCR was used to measure the expression levels of miR-155, miR-96 and miR-99a. The expression levels of miR-155 and miR-96 were upregulated in the tissues and serum of patients with HCC, whereas miR-99a expression levels were decreased. Receiver operating characteristics (ROC) curve analysis revealed that circulating miR-155, miR-96, miR-99a and a combination of these three miRNAs could serve as diagnostic biomarkers for HCC with areas under the curve (AUC) of 0.84, 0.824, 0.799 and 0.931, respectively. Serum α-fetoprotein (AFP) was detected using electrochemiluminescence immunoassay analyzer. The addition of AFP with the combination of these three miRNAs offered a higher accuracy of HCC diagnosis (AUC, 0.979; sensitivity, 90.0%; specificity, 100.0%). In addition, elevated expression levels of miR-155 and miR-96 were associated with poor survival time of patients with HCC. The panel of miR-155, miR-96, miR-99a and AFP had a higher sensitivity and specificity for the diagnosis of HCC when compared with a single marker. Furthermore, the present data suggested that miR-155 and miR-96 may be potential prognostic markers for the clinical management of patients with HCC.

9.
Sheng Li Xue Bao ; 71(4): 625-636, 2019 Aug 25.
Artigo em Chinês | MEDLINE | ID: mdl-31440760

RESUMO

Mitochondrial dynamics, involving mitochondrial fusion, fission and autophagy, plays an important role in maintaining cellular physiological function and homeostasis. Mitochondria are the "energy plant" of human body, so the changes of mitochondrial fusion, division and autophagy are important for cell respiration and energy production. On the other hand, energy metabolism influences mitochondrial dynamics in turn. This paper reviewed the recent advances in studies on the relationship between energy metabolism and the proteins regulating mitochondrial fusion, fission and autophagy. The association of mitochondrial dynamics with electron chain complex expression, oxidative phosphorylation and ATP synthesis upon exercise intervention will provide theoretical references for the further studies in sports training and disease intervention.


Assuntos
Autofagia , Metabolismo Energético , Exercício , Mitocôndrias/fisiologia , Dinâmica Mitocondrial , Trifosfato de Adenosina/biossíntese , Humanos , Proteínas Mitocondriais/metabolismo
10.
Artigo em Inglês | MEDLINE | ID: mdl-31362347

RESUMO

The Chinese government projected 30% of total consumed potatoes as a staple food (PSF) by 2020. We comprehensively assessed the potential impacts of PSF on rice and flour consumption, rice and wheat planting, energy and nutrient supply, irrigation-water, chemical nitrogen (N), phosphorus pentoxide (P2O5) and potassium oxide (K2O) fertilizer inputs and total greenhouse gases (GHG) emission for potatoes, rice and wheat, by assuming different proportions of potato substitutes for rice and flour. The results showed that per capita, 2.9 ± 0.3 and 4.7 ± 0.5 kg more potatoes per year would enter the Chinese staple-food diet, under the government's target. PSF consumed are expected to reach 5.2 ± 0.7 Tg yr-1, equivalent to substituting potatoes for 4.2 ± 0.8-8.5 ± 0.8 Tg yr-1 wheat and 5.1 ± 0.9-10.1 ± 1.8 Tg yr-1 rice under different scenarios. While this substitution can increase the nutrient supply index by 63% towards nutrient reference values, it may induce no significant effect on staple-food energy supply with lower chemical fertilizer (except for K2O) and irrigation-water inputs and GHG emissions in different substitution scenarios than the business as usual scenario. The reduction in rice and wheat demands lead to wheat in the North China Plain and early rice decrease by 6.1-11.4% and 12.1-24.1%, respectively. The total GHG reduction is equal to 1.1-9.0% of CO2 equivalent associated with CH4 and N2O emitted from the Chinese agroecosystem in 2005. The saved irrigation water for three crops compared to 2012 reaches the total water use of 17.9 ± 4.9-21.8 ± 5.9 million people in 2015. More N fertilizer, irrigation-water, and GHG can be reduced, if the PSF ratio is increased to 50% together with potato yield improves to the optimal level. Our results implied that the PSF policy is worth doing not only because of the healthier diets, but also to mitigate resource inputs and GHG emissions and it also supports agricultural structure adjustments in the areas of irrigated wheat on the North China Plain and early rice across China, designed to increase the adaptability to climate change.


Assuntos
Produtos Agrícolas , Abastecimento de Alimentos , Política Nutricional , Solanum tuberosum , Agricultura/métodos , China , Fertilizantes/análise , Gases de Efeito Estufa , Nitrogênio/química , Óxido Nitroso/análise , Oryza , Triticum , Água
11.
Acta Neuropsychiatr ; 31(6): 316-324, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31405402

RESUMO

OBJECTIVE: To explore whether and how group cognitive-behavioural therapy (GCBT) plus medication differs from medication alone for the treatment of generalised anxiety disorder (GAD). METHODS: Hundred and seventy patients were randomly assigned to the GCBT plus duloxetine (n=89) or duloxetine group (n=81). The primary outcomes were Hamilton Anxiety Scale (HAMA) response and remission rates. The explorative secondary measures included score reductions from baseline in the HAMA total, psychic, and somatic anxiety subscales (HAMA-PA, HAMA-SA), the Hamilton Depression Scale, the Severity Subscale of Clinical Global Impression Scale, Global Assessment of Functioning, and the 12-item Short-Form Health Survey. Assessments were conducted at baseline, 4-week, 8-week, and 3-month follow-up. RESULTS: At 4 weeks, HAMA response (GCBT group 57.0% vs. control group 24.4%, p=0.000, Cohen's d=0.90) and remission rates (GCBT group 21.5% vs. control group 6.2%, p=0.004; d=0.51), and most secondary outcomes (all p<0.05, d=0.36-0.77) showed that the combined therapy was superior. At 8 weeks, all the primary and secondary significant differences found at 4 weeks were maintained with smaller effect sizes (p<0.05, d=0.32-0.48). At 3-month follow-up, the combined therapy was only significantly superior in the HAMA total (p<0.045, d=0.43) and HAMA-PA score reductions (p<0.001, d=0.77). Logistic regression showed superiority of the combined therapy for HAMA response rates [odds ratio (OR)=2.12, 95% confidence interval (CI) 1.02-4.42, p=0.04] and remission rates (OR=2.80, 95% CI 1.27-6.16, p=0.01). CONCLUSIONS: Compared with duloxetine alone, GCBT plus duloxetine showed significant treatment response for GAD over a shorter period of time, particularly for psychic anxiety symptoms, which may suggest that GCBT was effective in changing cognitive style.

12.
World J Clin Cases ; 7(15): 2075-2080, 2019 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-31423440

RESUMO

BACKGROUND: Adrenocortical cancer (ACC) is an infrequent and often aggressive malignancy with a very poor prognosis. It can be classified as functional or nonfunctional. Nonfunctional ACC is hampered by the absence of specific signs or symptoms; only abdominal pain with or without incidental adrenal occupation is typically present. CASE SUMMARY: We report a rare case of a patient with a 30 cm × 15 cm × 8 cm ectopic ACC on the anterior abdominal wall without organ adhesion. A 77-year-old male was admitted to our hospital because of a huge abdominal mass, which, by ultrasonography, had an unclear border with the liver. Computed tomography showed that the mass was not associated with any organ but was adherent to the anterior abdominal wall. The patient underwent tumor resection, and a postoperative pathology examination showed a neuroendocrine tumor, which was diagnosed as ACC. The patient was disease-free at the 9-mo follow up. CONCLUSION: The anterior abdominal wall is a rare site of ACC growth.

13.
J Environ Manage ; 249: 109180, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31400586

RESUMO

Nitrogen flows in urban food systems are attracting increasing concern. However, characteristics of nitrogen flow and systematic measures to reduce reactive nitrogen losses in the food systems of consumption-oriented cities in developing countries have not been well understood, especially in a quantitative way. This study empirically investigates the transforming nitrogen flows of an urban food system in a food-sink city in China, with a nitrogen metabolism model. Three types of nitrogen loads transfer are identified: from production to consumption side, between different environmental media, and from areas within to areas beyond the city boundary. By integrating sensitivity analysis into the metabolism model, increases in the sewage treatment rate, the sewage nitrogen removal rate, and the ratio of animal excreta returned to field are found to contribute the most to the water nitrogen load reduction, and reducing food waste at the consumer level is the most influential measure for lowering soil nitrogen loads, under the existing nitrogen flow regime. Additionally, a three-tier template framework is proposed to streamline city strategies (prevention, abatement, recycling, regional cooperation, etc.) for reducing the N loads of urban food systems, providing references for sustainable nutrient management in urban ecosystems.


Assuntos
Ecossistema , Nitrogênio , Animais , China , Cidades , Alimentos
14.
Zhongguo Zhong Yao Za Zhi ; 44(11): 2397-2402, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31359669

RESUMO

The purpose of this paper is to study the use rules of drugs for lung diseases in internal medicine department of Xin'an Wang's family, discuss the compatibility of common drugs for lung diseases, guide clinical application, and inherit Xin'an medicine. By retrospective study on lung diseases cases in Wang's internal medicine works, the lung diseases and use frequency of common drugs treated by Wang's medicine were counted, and the systematic clustering and association rule analysis of common drugs were conducted by using SPSS Statistic 20 and SPSS Modeler 18.0, respectively. The results showed that asthma, cold and cough were the main lung diseases treated by Wang's medicine, and the commonly used medicines included antitussive and antiasthmatic drugs, spleen-invigorating and dampness-removing drugs, and expectorants. The medicine taste was mainly bitter, pungent and sweet, with cold and warm properties in a balanced way, without severely cold or hot herbs, mainly attributing to the lung and stomach meridians. In clustering analysis, 10 drug combinations were obtained; association analysis showed that two, three, four association rules respectively had 11, 21, and 10 groups, and each drug group had 11, 16, and 5 items. Core combinations: Poria, Armeniacae Semen Amarum, Asteris Radix et Rhizome, Coicis Semen, Farfarae Flos, Dendrobii Caulis, Perilla Frutescens, Stemonae Radix, Citri Reticulatae Pericarpium, Cynanchi Stauntonii Rhizome et Radix, Meretricis Concha Cyclinae Concha, Belamcandae Rhizoma, and Pinelliae Rhizome. Xin'an Wang's medicine paid attention to the lung nature when treating lung diseases. Lung is a delicate organ, not resistant to coldness or heat, so severely cold or hot herbs shall not be used, and the clear and light drugs with functions of dispersing lung Qi, clearing phlegm evil, strengthening spleen, eliminating phlegm, and relieving cough and asthma are often used. Lung deficiency is a kind of deficiency of Qi and Yin, so both Qi and Yin shall be regulated. Deficiency of Yin would burn the lung and make the lung collaterals blocked. In this case, the lung collaterals shall be dredged for hemostasis. Long time of lung deficiency would hinder the distribution of body fluid, and lung shall be regulated to dissipate phlegm.


Assuntos
Mineração de Dados , Medicamentos de Ervas Chinesas/uso terapêutico , Pneumopatias/tratamento farmacológico , Humanos , Medicina Tradicional Chinesa , Meridianos , Estudos Retrospectivos
15.
World J Gastroenterol ; 25(24): 3044-3055, 2019 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-31293340

RESUMO

BACKGROUND: The formation of liver fibrosis is mainly caused by the activation of hepatic stellate cells (HSCs) and the imbalance of extracellular matrix (ECM) production and degradation. The treatment of liver fibrosis mainly includes removing the cause, inhibiting the activation of HSCs, and inhibiting inflammation. NOD-like receptor (NLR) family, caspase activation and recruitment domain (CARD) domain containing 5/NOD27/CLR16.1 (NLRC5) is a highly conserved member of the NLR family and is involved in inflammation and immune responses by regulating various signaling pathways such as nuclear factor-κB (NF-κB) signaling. It has been found that NLRC5 plays an important role in liver fibrosis, but its specific effect and possible mechanism remain to be fully elucidated. AIM: To investigate the role of NLRC5 in the activation and reversion of HSCs induced with transforming growth factor-ß (TGF-ß) and MDI, and to explore its relationship with liver fibrosis. METHODS: A total of 24 male C57BL/6 mice were randomly divided into three groups, including normal, fibrosis, and recovery groups. Twenty-four hours after a liver fibrosis and spontaneous reversion model was established, the mice were sacrificed and pathological examination of liver tissue was performed to observe the degree of liver fibrosis in each group. LX-2 cells were cultured in vitro and treated with TGF-ß1 and MDI. Real-time quantitative PCR (qPCR) and Western blot were used to analyze the expression levels of NLRC5, α-smooth muscle actin (α-SMA), and collagen type I alpha1 (Col1a1) in each group. The activity of NF-κB in each group of cells transfected with NLRC5-siRNA was detected. RESULTS: Compared with the normal mice, the expression level of NLRC5 increased significantly (P < 0.01) in the fibrosis group, but decreased significantly in the recovery group (P < 0.01). In in vitro experiments, the content of NLRC5 was enhanced after TGF-ß1 stimulation and decreased to a lower level when treated with MDI (P < 0.01). The expression of α-SMA and Col1a1 proteins and mRNAs in TGF-ß1-mediated cells was suppressed by transfection with NLRC5-siRNA (P < 0.01). Western blot analysis showed that the expression of NF-κB p65 protein and phosphorylated IκBα (p-IκBα) was increased in the liver of mice in the fibrosis group but decreased in the recovery group (P < 0.01), and the protein level of nuclear p65 and p-IκBα was significantly increased after treatment with NLRC5-siRNA (P < 0.01). CONCLUSION: NLRC5 may play a key role in the development and reversal of hepatic fibrosis through the NF-κB signaling pathway, and it is expected to be one of the clinical therapeutic targets.


Assuntos
Células Estreladas do Fígado/patologia , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Cirrose Hepática Experimental/patologia , NF-kappa B/metabolismo , Animais , Tetracloreto de Carbono/toxicidade , Linhagem Celular , Matriz Extracelular/patologia , Técnicas de Silenciamento de Genes , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/genética , Fígado/citologia , Fígado/patologia , Cirrose Hepática Experimental/induzido quimicamente , Masculino , Camundongos , RNA Interferente Pequeno/metabolismo , Proteínas Recombinantes/metabolismo , Transdução de Sinais , Fator de Crescimento Transformador beta1/metabolismo
18.
Zhongguo Ying Yong Sheng Li Xue Za Zhi ; 35(2): 126-129, 2019 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-31250602

RESUMO

OBJECTIVE: To investigate the effects of intermittent negative pressure therapy on the skin microcirculation perfusion of quadriceps in male rowers, and to provide basis for the practical application of this method. METHODS: Fourteen male rowers were selected from the national rowing team and randomly divided into experimental and control groups. The daily training plans of two groups were the same. The recovery intervention for experimental group was implemented by 20 minutes in the cube of Vacusport Regeneration System (German), 5 times per week for 4 weeks, no recovery intervention for control group. Microcirculation markers were collected by PeriFlux5000 system before and after the 4-week intervention. The markers included microcirculatory blood perfusion(MBP), average velocity of blood cells(AVBC), concentration of moving blood cells (CMBC), and values of the markers included basic values and post-heating values (44℃), difference before and after heating of the values was considered as the reserve capacity of those markers. RESULTS: The test results before the 4 weeks intervention showed there was no statistical difference between the two groups(P>0.05). After the 4 weeks intervention: ①MBP: The post-heating value and the difference of the experimental group were significantly higher than those of the control group (P<0.05). But there was no statistical inner-group difference. ②AVBC: The post-heating values and the difference in the experimental group were significantly lower than those in the control group (P<0.05). Intra-group comparison found that the post-heating values after post-intervention were significantly reduced, compared with those of pre-intervention (P< 0.01); the difference after post-intervention was reduced significantly, compared with those in the pre-intervention (P<0.05). ③CMBC: The post-heating values and the difference in the experimental group were significantly higher than those in the control group (P<0.01). There were no statistical significant inner-group difference. CONCLUSION: Lower limb intermittent negative pressure therapy can improve the skin microcirculation of the quadriceps of the male rowers, which has a positive effect on the rapid recovery of physical fitness.


Assuntos
Microcirculação , Pressão , Músculo Quadríceps/irrigação sanguínea , Pele/irrigação sanguínea , Esportes Aquáticos , Humanos , Extremidade Inferior , Masculino
19.
Nanomedicine (Lond) ; 14(12): 1521-1531, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31180256

RESUMO

Aim: To investigate the enhancement of bioavailability by the usage of drug nanoparticles for increasing the efficacy of antidepressant therapeutic value. Materials & methods: Nano-amitriptyline HCI (AMT·HCl) particles were successfully prepared via a simple spray freeze drying (SFD) method. Results: The as-prepared nanoparticles are amorphous instead of crystalline. The mean size of AMT·HCl nanoparticles is 90 nm. In in vitro evaluation, AMT·HCl nanoparticles have greatly improved the dissolution compared with pure bulk materials, which have potential for enhancing human bioavailability and diminishing toxic effect. A nanoparticle formation mechanism was also proposed. Conclusion: These findings promote the development of antidepressant therapeutic evaluation based on the usage of AMT·HCl nanoparticles by SFD method and indicate that SFD is an alternative for a range of nanoparticle preparation in industrial pharmacy.

20.
J Cell Biochem ; 120(9): 14604-14617, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31148248

RESUMO

Chronic kidney disease (CKD) is a traumatic disease with significant psychic consequences to the patient's overall physical condition. microRNA-206 (miR-206) has been reported to play an essential role in the development of various diseases. The purpose of the present study is to investigate the effect of miR-206 through the JAK/STAT signaling pathway on epithelial-mesenchymal transition (EMT) of renal tubular epithelial cells and glomerulosclerosis in rats with CKD. The targeting relationship between miR-206 and ANXA1 was verified. To explore the role of miR-206 in CKD, the model of CKD rats was established to detect glomerular sclerosis index (GSI), contents of interleukin-6 (IL-6) and transforming growth factor-beta1 (TGF-ß1), and expression of type IV collagen. Moreover, to further determine the roles of both miR-206 and the JAK/STAT signaling pathway in CKD, the gain- and loss-of function approaches were performed with the expression of ANXA1, α-SMA, E-cadherin, vimentin, N-cadherin, and the JAK/STAT signaling pathway-related genes detected. miR-206 negatively targeted ANXA1. Overexpressed miR-206 inhibited the degeneration and interstitial fibrosis of renal tubular epithelial cells, decreased GSI of rats, and the expression of type IV collagen, TGF-ß1 and IL-6. Overexpressed miR-206 inhibited the degeneration of renal tubular epithelial cells, the expression of ANXA1, α-SMA, TGF-ß1, p-STAT3, STAT3, p-STAT1, STAT1, p-JAK2, and JAK2, while promoted the expression of E-cadherin. Taken together the results, miR-206 inhibits EMT of renal tubular epithelial cells and glomerulosclerosis by inactivating the JAK/STAT signaling pathway via ANXA1 in CKD.

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