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1.
J Org Chem ; 2022 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-36098507

RESUMO

A demethylative silyl radical cascade cyclization of 2-isocyanothioanisoles toward 2-silylated benzothiazole building blocks has been developed. The development of a "radical initiator-inhibitor symbiosis" system solves the challenge of otherwise dominant methyl radical-triggered side reactions brought about by kinetically unfavored generation of reactive silyl radical species. The products accessed in this protocol are amendable to various downstream functionalization reactions, including the quick construction of a topoisomerase II inhibitor via a Hiyama cross-coupling reaction and of an antiviral agent via a fluoride-/hydroxide-free nucleophilic substitution to acyl chloride.

2.
Arch Public Health ; 80(1): 209, 2022 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-36115969

RESUMO

BACKGROUND: Regularly updated epidemiological data on the burden of brain and central nervous system (CNS) cancers are important in the prioritization of research and the allocation of resources. This study aimed to investigate incidence, mortality, disability, and trends in brain and CNS cancers between 1990 and 2019. METHODS: Epidemiological data, including the cancer incidence, mortality, disability-adjusted life years(DALYs), age-standardized incidence rate (ASIR), age-standardized mortality rate (ASMR), and age-standardized DALY rate (per 100,000 population) stratified by region, country, sex, and age group were retrieved and extracted using the Global Health Data Exchange (GHDx) query tool. RESULTS: In 2019, there were 347,992(262,084-388,896) global cases of brain and CNS cancers, which showed a significant increase (94.35%) from the period between 1990 to 2019. The global ASIR in 2019 was 4.34 (3.27-4.86) per 100, 000 population, which showed an increasing trend for the years 1990-2019 (13.82% [-27.27-32.83]). In 2019, there were 246,253 (185,642-270,930) global deaths caused by brain and CNS cancers, which showed a significant increase (76.36%) during the study period. The global ASMR in 2019 was 3.05(2.29-3.36) per 100, 000 population, which did not change significantly over the study period (-1.19% [-36.79-13.86]). In 2019, there were 8,659,871 DALYs, which was a 109.04% increase compared with 1990. Similarly, during 1990-2019, the age-standardized DALY rate decreased by 10.39%. Additionally, 76.60% of the incident cases, 72.98% of the deaths, and 65.16% of the DALYs due to brain and CNS cancers occurred in the high-income and upper-middle-income regions. CONCLUSIONS: In conclusion, brain and CNS cancers remain a major public health burden, particularly in high-income regions. The global incidence, deaths, and DALYs of brain and CNS cancers were shown to have increased significantly from 1990 to 2019. The global ASIR kept rising steadily, while the ASMR and age-standardized DALY rate declined over the past three decades.

3.
Artigo em Inglês | MEDLINE | ID: mdl-36102873

RESUMO

Different from organic dye/quantum dot possessing one luminescent center, upconversion luminescence (UCL) is actually a temporal statistic of countless individual activators. Our experimental results have shown that the rise and decay dynamics of UCL is directly associated with the relative contribution of sensitizer-to-activator energy transfer and energy migration among sensitizers, which can be physically modulated by tuning the excitation laser. Therefore, dynamic UCL with record-wide 20-fold lifetime, ~70-fold red-to-green intensity ratio, and reversibly definable emission color is easily realized by just modulating the excitation laser. Moreover, this generally applicable strategy only requires a simplest-possible UCL system whereas prevalent material engineering such as complicated composition design, sophisticated core-shell construction, or tedious chemical synthesis, is no longer needed.

4.
J Neuroinflammation ; 19(1): 215, 2022 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-36058959

RESUMO

Alzheimer's disease (AD) is the most common neurodegenerative disease and the most common cause of dementia. Among various pathophysiological aspects, microglia are considered to play important roles in the pathogenesis of AD. Genome wide association studies (GWAS) showed that the majority of AD risk genes are highly or exclusively expressed in microglia, underscoring the critical roles of microglia in AD pathogenesis. Recently, omics technologies have greatly advanced our knowledge of microglia biology in AD. Omics approaches, including genomics, epigenomics, transcriptomics, proteomics, and metabolomics/lipidomics, present remarkable opportunities to delineate the underlying mechanisms, discover novel diagnostic biomarkers, monitor disease progression, and shape therapeutic strategies for diseases. In this review, we summarized research based on microglial "omics" analysis in AD, especially the recent research advances in the identification of AD-associated microglial subsets. This review reinforces the important role of microglia in AD and advances our understanding of the mechanism of microglia in AD pathogenesis. Moreover, we proposed the value of microglia-based omics in the development of therapeutic strategies and biomarkers for AD.


Assuntos
Doença de Alzheimer , Doenças Neurodegenerativas , Doença de Alzheimer/genética , Doença de Alzheimer/patologia , Biomarcadores , Estudo de Associação Genômica Ampla , Humanos , Microglia/patologia , Doenças Neurodegenerativas/patologia
5.
Micromachines (Basel) ; 13(9)2022 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-36144050

RESUMO

To address the problems in the calibration of soil water content sensors, in this study, we designed a low-cost edge electromagnetic field induction (EEMFI) sensor for soil water content measurement and proposed a normalized calibration method to eliminate the errors caused by the measurement sensor's characteristics and improve the probe's consistency, replaceability, and calibration efficiency. The model calibration curve-fitting coefficients of the EEMFI sensors were above 0.98, which indicated a significant correlation. The experimental results of the static and dynamic characteristics showed that the measurement range of the sensor varied from 0% to 100% saturation, measurement accuracy was within ±2%, the maximum value of the extreme difference of the stability test was 1.09%, the resolution was 0.05%, the delay time was 3.9 s, and the effective measurement diameter of the EEMFI sensor probe was 10 cm. The linear fit coefficient of determination of the results was greater than 0.99, and the maximum absolute error of the measurement results with the drying method was less than ±2%, which meets the requirements of soil water content measurement in agriculture and forestry fields. The field experiment results further showed that the EEMFI sensor can accurately respond to changes in soil water content, indicating that the EEMFI sensor is reliable.

6.
Int Wound J ; 2022 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-36148625

RESUMO

This study aimed to explore the clinical application and efficacy of traditional Chinese medicine (TCM) powder in the treatment of acute and chronic wounds. Eighty patients with a wound infection were randomly and equally divided into a control group and an observation group. Gauze padding containing furacilin was used to dress the infected wounds of the control group. TCM powder was used to treat the wounds of the observation group. The total response rate of the observation group was significantly higher than the control group (P = .017). The colour and exudate volume scores in the observation group were lower than the control group, and the differences between the two groups were statistically significant (P < .05). The time to the appearance of new epithelium and time to the wound healing of the burns in the observation group were shorter than the control group, and the differences were statistically significant (P < .05). The TCM powder absorbed a large amount of necrotic tissue and exudate from the wound surface, cleared heat and toxins, and activated blood circulation. It also resolved blood stasis, eliminated pus, and allowed for new skin growth, as well as regenerating muscle.

7.
Nanomicro Lett ; 14(1): 192, 2022 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-36121520

RESUMO

Eliminating topological defects to achieve monodomain liquid crystals is highly significant for the fundamental studies of soft matter and building long-range ordered materials. However, liquid crystals are metastable and sensitive to external stimuli, such as flow, confinement, and electromagnetic fields, which cause their intrinsic polycrystallinity and topological defects. Here, we achieve the monodomain liquid crystals of graphene oxide over 30 cm through boundary-free sheargraphy. The obtained monodomain liquid crystals exhibit large-area uniform alignment of sheets, which has the same optical polarized angle and intensity. The monodomain liquid crystals provide bidirectionally ordered skeletons, which can be applied as lightweight thermal management materials with bidirectionally high thermal and electrical conductivity. Furthermore, we extend the controllable topology of two-dimensional colloids by introducing singularities and disclinations in monodomain liquid crystals. Topological structures with defect strength from - 2 to + 2 were realized. This work provides a facile methodology to study the structural order of soft matter at a macroscopic level, facilitating the fabrication of metamaterials with tunable and highly anisotropic architectures.

9.
Front Aging Neurosci ; 14: 957997, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36118705

RESUMO

Objective: This study aimed to investigate the association of altered cortical thickness and functional connectivity (FC) with depression in Parkinson's disease (PD). Materials and methods: A total of 26 non-depressed PD patients (PD-ND), 30 PD patients with minor depression (PD-MnD), 32 PD patients with major depression (PD-MDD), and 30 healthy controls (HC) were enrolled. Differences in cortical thickness among the four groups were assessed, and the results were used to analyze FC differences in regions of cortical atrophy. Binary logistic regression and receiver operating characteristic (ROC) curve analyses were also performed to identify clinical features and neuroimaging biomarkers that might help in the prediction of PD-MDD. Results: Patients with PD-MDD showed decreased cortical thickness compared to patients with PD-ND in the left superior temporal and right rostral middle frontal gyri (RMFG), as well as weak FC between the left superior temporal gyrus and right cerebellum posterior lobe and between right RMFG and right inferior frontal gyrus and insula. The combination of cortical thickness, FC, and basic clinical features showed strong potential for predicting PD-MDD based on the area under the ROC curve (0.927, 95% CI 0.854-0.999, p < 0.001). Conclusion: Patients with PD-MDD show extensive cortical atrophy and FC alterations, suggesting that cortical thickness and FC may be neuroimaging-based diagnostic biomarkers for PD-MDD.

10.
Artigo em Inglês | MEDLINE | ID: mdl-36062173

RESUMO

Purpose: To analyse the efficacy of high-dose methotrexate + adriamycin + cisplatin (HD-MTX + ADR + PDD, MAP) regimens applied to osteosarcoma and the pretreatment and resolution of chemotherapeutic reactions. Methods: The clinical data of 21 patients with osteosarcoma in our hospital from January 2015 to January 2018 were retrospectively analysed. All patients were treated with the MAP protocol, 21 days for 1 cycle, and treated with artificial joint replacement or amputation after 3∼4 cycles of treatment. The tumour tissue necrosis rate, limb preservation success rate after treatment, and chemotherapy response during chemotherapy were counted and analysed for all patients. A local recurrence rate, a distant metastasis rate, and an overall survival rate were recorded during the 3-year follow-up period. Results: After treatment, the percentage of tumour tissue necrosis ≥90% was 85.71% (18/21) and the percentage of successful limb preservation was 57.14% (12/21) in 21 patients with osteosarcoma. During chemotherapy, all 21 patients with osteosarcoma experienced various degrees of chemotherapy reactions, mainly bone marrow suppression of 100% (21/21), gastrointestinal reactions of 100% (21/21), liver function impairment of 66.67% (14/21), and cardiotoxicity of 52.38% (11/21), all of which improved and completed treatment after treatment. During the 3-year follow-up period, the 21 patients with osteosarcoma had a local recurrence rate of 9.52% (2/21), a distant metastasis rate of 28.57% (6/21), and an overall survival rate of 80.95% (17/21). Conclusion: With stringent protection and relief measures, patients with osteosarcoma treated with the MAP regimen have promising near-term outcomes, with high survival rates over 3 years and tolerable chemotherapy responses. The clinical trial is registered under L2015093.

11.
J Oleo Sci ; 2022 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-36089399

RESUMO

Renal tissue plays a crucial function in maintaining homeostasis, making it vulnerable to xenobiotic toxicity. Pueraria montana has more beneficial potential against the various diseases and has long history used as a traditional Chinese medicine. But its effect against the renal cancer not scrutinize. The goal of this study is to see if Pueraria montana can protect rats from developing kidney tumors caused by diethylnitrosamine (DEN) and ferric nitrite (Fe-NTA). Wistar rats was selected for the current study and DEN (use as an inducer) and Fe-NTA (promoter) for induction the renal cancer. For 22 weeks, the rats were given oral Pueraria montana (12.5, 25, and 50 mg/kg) treatment. At regular intervals, the body weight and food intake were calculated. The rats were macroscopically evaluated for identification of cancer in the renal tissue. The renal tumor makers, renal parameters, antioxidant enzymes, phase I and II enzymes, inflammatory cytokines and mediators were estimated at end of the experimental study. Pueraria montana treated rats displayed the suppression of renal tumors, incidence of the tumors along with suppression of tumor percentage. Pueraria montana treated rats significantly (p < 0.001) increased body weight and suppressed the renal weight and food intake. It also reduced the level of renal tumor marker ornithine decarboxylase (ODC) and [3H] thymidine incorporation along with suppression of renal parameter such as uric acid, blood urea nitrogen (BUN) and urea and creatinine. Pueraria montana treatment significantly (p < 0.001) altered the level of phase enzymes and antioxidant. Pueraria montana treatment significantly (p < 0.001) repressed the level of tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß), interleukin-6 (IL-6) and improved the level of interleukin-10 (IL-10). Pueraria montana treatment suppressed the level of prostaglandin (PGE2), cyclooxygenase-2 (COX-2), nuclear kappa B factor (NF-κB) and transforming growth factor beta 1 (TGF-ß1). Pueraria montana suppressed the inflammatory necrosis, size the bowman capsules in the renal histopathology. Pueraria montana exhibited the chemoprotective effect via dual mechanism such as suppression of inflammatory reaction and oxidative stress.

12.
Polymers (Basel) ; 14(16)2022 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-36015681

RESUMO

The safety and stability of power systems are seriously threatened by the decay-like fracture of composite insulators. This paper analyzes the aging characteristics (physical properties, NMR, and FTIR) of the silicone rubber sheds of the decay-like fractured insulator. The same V-string insulator and a new insulator are used for comparison. The study shows that the sheds' degradation is concentrated on the side with heavy pollution. The physical properties (appearance, pollution, hardness, and hydrophobicity) of the high voltage end decrease significantly compared to other positions, but there is no direct connection between the physical properties of sheds and the decay-like fracture of the core rod. The severity of aging increases with a decrease in the equivalent transverse relaxation time T2. The main chain of the PDMS material was severely damaged at the location of the insulator fracture. NMR and FTIR can well judge the aging degree of silicone rubber housings. However, no definite characteristic quantity can characterize the decay-like fracture. It is challenging to evaluate the decay-like fracture of the silicone rubber shed only by its aging degree.

13.
Reprod Fertil Dev ; 2022 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-36031717

RESUMO

CONTEXT: Low ovarian putrescine levels and decreased peak values following luteinising hormone peaks are related to poor oocyte quantity and quality in ageing women. AIMS: To investigate the effects of putrescine supplementation in in vitro maturation (IVM) medium on oocyte quality and epigenetic modification. METHODS: Germinal vesicle oocytes retrieved from the ovaries of 8-week-old and 9-month-old mice were divided into four groups (the young, young+difluoromethylornithine (DFMO), ageing and ageing+putrescine groups) and cultured in IVM medium with or without 1mM putrescine or DFMO for 16h. The first polar body extrusion (PBE), cleavage and embryonic development were evaluated. Spindles, chromosomes, mitochondria and reactive oxygen species (ROS) were measured. The expression levels of SIRT1, H3K9ac, H3K9me2, H3K9me3, and 5mC levels were evaluated. Sirt1 and imprinted genes were detected. RESULTS: The PBE was higher in the ageing+putrescine group than in the ageing group. Putrescine increased the total and inner cell mass cell numbers of blastocysts in ageing oocytes. Putrescine decreased aberrant spindles and chromosome aneuploidy, increased the mitochondrial membrane potential and decreased ROS levels. Putrescine increased SIRT1 expression and attenuated the upregulation of H3K9ac levels in ageing oocytes. Putrescine did not affect 5mC, H3K9me2 or H3K9me3 levels or imprinted gene expression. CONCLUSIONS: Putrescine supplementation during IVM improved the maturation and quality of ageing oocytes and promoted embryonic development by decreasing ROS generation, maintaining mitochondrial and spindle function and correcting aberrant epigenetic modification. IMPLICATIONS: Putrescine shows application potential for human-assisted reproduction, especially for IVM of oocytes from ageing women.

14.
Artigo em Inglês | MEDLINE | ID: mdl-35954743

RESUMO

According to recent research, the COVID-19 pandemic has impacted road traffic quality. This study aims to analyze the impacts of COVID-19 travel restriction policies on the traffic quality of the national and provincial trunk highway network (NPTHN) in Shaanxi Province. We collected the traffic data of the NPTHN for three consecutive years (from 2019 to 2021), before and after the COVID-19 outbreak, including weekly average daily traffic, weekly traffic interruption times, weekly traffic control time, weekly traffic accidents, weekly traffic injuries, and weekly traffic deaths. Using descriptive statistics and dynamic analysis methods, we studied the safety and service levels of the NPTHN. We set up an assessment model of the NPTHN operational orderliness through dissipative structure theory and entropy theory to study the operational orderliness of the NPTHN. Results show that in 2020, the service level, safety level, and operational orderliness of the NPTHN dropped to the lowest levels. The pandemic was gradually brought under control, and the travel restriction policies were gradually reduced and lifted. The adverse impacts on the operational orderliness of the NPTHN decreased, but the operational orderliness did not yet recover to the pre-pandemic level. Meanwhile, the service and safety levels of the NPTHN did not recover. Taken together, the COVID-19 travel restriction policies had adverse impacts on the traffic quality of the NPTHN in Shaanxi Province.


Assuntos
COVID-19 , Acidentes de Trânsito , COVID-19/epidemiologia , Humanos , Pandemias , Políticas , Viagem
15.
Artigo em Inglês | MEDLINE | ID: mdl-36001991

RESUMO

AIMS: The aim of this study was to investigate the impact on 10-year survival of patient-reported anginal status at 1-year following percutaneous coronary intervention (PCI) or coronary artery bypass graft (CABG) in patients with left main coronary artery disease (LMCAD) and/or 3-vessel CAD (3VD). METHODS AND RESULTS: In this post-hoc analysis of the randomized SYNTAX Extended Survival study, patients were classified as having residual angina (RA) if their self-reported Seattle Angina Questionnaire angina frequency scale (SAQ-AF) was ≤ 90 at 1-year follow-up post revascularization with PCI or CABG. The primary endpoint of all-cause death at 10 years was compared between the RA and no-RA groups. A sensitivity analysis was performed using 6-month SAQ-AF.At 1-year, 373 (26.1%) out of 1428 patients reported RA. Whilst RA at 1-year was an independent correlate of repeat revascularization at 5 years (18.3% vs. 11.5%; adjusted hazard ratio [HR]: 1.54; 95% confidence interval [CI]: 1.10-2.15), it was not associated with all-cause death at 10 years (22.1% vs. 21.6%; adjusted HR: 1.11; 95%CI: 0.83-1.47). These results were consistent when stratified by modality of revascularization (PCI or CABG) or by anginal frequency. The sensitivity analysis replicating the analyses based on 6-month angina status resulted in similar findings. CONCLUSION: Among patients with LMCAD and/or 3VD, patient-reported RA at 1-year post revascularization was independently associated with repeat revascularization at 5 years, however it did not significantly increase 10-year mortality, irrespective of the primary modality of revascularization.

16.
Front Oncol ; 12: 936507, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35978818

RESUMO

Objective: Cholangiocarcinoma (CHOL) is a deadly cancer worldwide with limited available therapies. The aim of this study was to investigate key exosomal miRNAs and their functions in CHOL development. Methods: Serum exosomes were isolated from patients with CHOL and healthy controls, followed by miRNA sequencing for identifying differentially expressed miRNAs (DEMs) and their functions. Then, the expression of key DEMs was experimentally validated in exosomes from clinical CHOL patients and CHOL cells. The effects of overexpression of key DEMs on CHOL cell migration and proliferation were investigated. A key exosomal DEM miR-3124-5p was identified. The effects of overexpression or knockdown of exosomal miR-3124-5p on the proliferation, migration, and angiogenesis of human umbilical vein endothelial cells (HUVECs) were investigated. Moreover, the function of exosomal miR-3124-5p on tumor growth in vivo was explored. Results: A total of 632 exosomal DEMs were identified between CHOL and control samples. Target genes of DEMs were significantly enriched in pathways, such as the p53 signaling pathway. miR-3124-5p was upregulated in serum exosomes from CHOL patients and exosomes from CHOL cells, and overexpression of miR-3124-5p promoted RBE cell migration and viability. Moreover, overexpression of exosomal miR-3124-5p promoted the proliferation, migration, and angiogenesis of HUVECs, while knockdown of miR-3124-5p had the opposite effect. miR-3124-5p could target growth differentiation factor 11 (GDF11) and downregulate GDF11 expression. Furthermore, exosomal miR-3124-5p promoted tumor growth in vivo. Conclusions: Our findings revealed that exosome-encapsulated miR-3124-5p promoted the malignant progression of CHOL by targeting GDF11. Exosomal miR-3124-5p and GDF11 could be promising biomarkers or therapeutic targets for CHOL.

17.
ACS Nano ; 2022 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-36001475

RESUMO

Constructing conductive filler networks with high efficiency is essential to fabricating functional polymer composites. Although two-dimensional (2D) sheets have prevailed in nanocomposites, their efficiency in enhancing conductive functions seems to reach a limit, as if merely addressing the dispersion homogeneity. Here, we exploit the unrecognized geometric curvature of 2D sheets to break the efficiency limit of filler systems. We introduce the hyperbolic curvature concept to mediate the incompatibility between 2D planar topology and 3D filler space and hold the efficient conductive path through face-to-face contact. The hyperbolic graphene framework exhibits a record efficiency in enhancing electrically and thermally conductive functions of nanocomposites. At a volume loading of only 1.6%, the thermal and electrical conductivities reach 31.6 W/(mK) and 13 911 S/m, respectively. We demonstrate that the conductive nanocomposites with a hyperbolic graphene aerogel framework are useful for thermal management, sensing, and electromagnetic shielding. Our work provides a solution to reconcile the incompatibility between the 2D planar structure of sheets and the highly expected 3D conductive path, presenting a geometrically optimal filler system to break the efficiency limit of multifunctional nanocomposites and broaden the structural design space of 2D sheets by curvature modulation to meet more applications.

18.
PLoS One ; 17(8): e0266519, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35994492

RESUMO

The friction coefficient between the tire and the road is one of the key parameters affecting road traffic safety. The purpose of this paper is to quantify the risk of skidding for the vehicles due to the friction evolution caused by the traffic polishing in the horizontal curve. Based on the reliability theory, an innovative dynamic risk assessment model is developed in the present study for passenger cars and trucks. The influence of two traffic characteristics for pavement friction is quantified: cumulative traffic volume (CTV) and annual average daily traffic of trucks (AADTT). The speed distribution on the horizontal curve of the motorway is obtained through field experiments as the basic parameter of the model. The Hasofer-Lind Method is adopted to solve the reliability and the risk probability of vehicle skidding. The results show that in the traffic characteristics, the AADTT has a significant impact on the pavement friction; When the AADTT on the road exceeds 2000 veh/d, the increasing CTV leads to friction decrease rapidly and therefore has a significant impact on the risk of horizontal curve. Especially for roads with more than 50 million vehicles of the CTV, the risk of the horizontal curve shows a sharp increase with CTV rising. The research results can provide reference for the road maintenance department to determine the timing of road maintenance.


Assuntos
Condução de Veículo , Acidentes de Trânsito , Fricção , Reprodutibilidade dos Testes , Segurança
19.
J Environ Manage ; 320: 115805, 2022 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-35930883

RESUMO

Water scarcity and water pollution problems are becoming increasingly serious in many regions of the world due to rapid socioeconomic development. Water resources environmental carrying capacity (WRECC), which embodies the attributes of social, economic, resource and environmental aspects, is usually considered as a significant metric to guide regional sustainable development. Most existing studies on WRECC mainly focus on conceptual discussions and evaluation methods, lacking a systematic insight into the theoretical connotations of WRECC and neglecting the interaction mechanisms within the WRECC system. Therefore, this study aims to propose a new WRECC evaluation method based on support and pressure theory to deeply reveal the state change and driving mechanisms of WRECC. Firstly, a WRECC evaluation system incorporating support and pressure is constructed and the WRECC performance is determined by both the support index and pressure index. Subsequently, the catastrophe progression method and the obstacle degree model are introduced to quantify the WRECC and identify the primary influencing factors. Lastly, the feasibility and validity of this methodology are verified through an empirical application in the Guangdong-Hong Kong-Macao Greater Bay Area (GBA). The results indicate that the WRECC of the GBA presents an upward trend from 2010 to 2019, shifting from a low-value load state to a low-value surplus state. Moreover, the WRECC performance varies significantly among cities in the GBA, especially for edge cities that generally exhibit higher support, pressure and WRECC indices than central cities. In addition, common obstacles that affect WRECC are total water resources, water resources per capita, water consumption per hectare for agriculture and proportion of the tertiary industry. Furthermore, this study reveals that the coupling relationship between support and pressure develops towards a positive direction, and water environment protection and economic development are the main drivers of WRECC development. This new proposed methodology can provide a theoretical reference for investigating regional WRECC and formulating appropriate sustainable development strategies.


Assuntos
Conservação dos Recursos Naturais , Recursos Hídricos , China , Cidades , Hong Kong , Macau , Água
20.
J Surg Res ; 279: 619-632, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-35926312

RESUMO

INTRODUCTION: The objective of our study was to explore the expression pattern of circular ribonucleic acid (RNA)_0,007,331 (circ_0,007,331) in breast cancer (BC) and its functional association with cellular paclitaxel (PTX) resistance and proliferation, migration, invasion and apoptosis. METHODS: Real-time quantitative polymerase chain reaction was applied to measure RNA expression. The PTX resistance of BC cells was analyzed by cell counting kit-8 assay. Flow cytometry was applied to assess cell cycle progression and cell apoptosis. Transwell assays were utilized to analyze cell migration and invasion abilities. Protein expression was determined by Western blot assay. The target relationship between microRNA-200b-3p (miR-200b-3p) and circ_0,007,331 or Anillin (ANLN) was verified by dual-luciferase reporter assay and RNA-pull down assay. The in vivo role of circ_0,007,331 was analyzed using xenograft tumor model. RESULTS: Circ_0,007,331 expression was elevated in PTX-resistant BC cell lines relative to parental BC cell lines. Circ_0,007,331 contributed to the PTX resistance, proliferation, migration, invasion and suppressed the apoptosis of BC cells. Circ_0,007,331 interacted with miR-200b-3p in BC cells. Circ_0,007,331 silencing-mediated effects in BC cells were largely overturned by the knockdown of miR-200b-3p. ANLN was a target of miR-200b-3p in BC cells. Circ_0,007,331 silencing reduced ANLN expression partly through upregulating miR-200b-3p in BC cells. miR-200b-3p overexpression-induced effects in BC cells were largely counteracted by the accumulation of ANLN. Circ_0,007,331 silencing aggravated PTX-mediated inhibitory effect on tumor growth in vivo. CONCLUSIONS: Circ_0,007,331 contributed to the PTX resistance, proliferation and motility and inhibited the apoptosis of BC cells through mediating miR-200b-3p/ANLN signaling.


Assuntos
Neoplasias da Mama , MicroRNAs , Neoplasias da Mama/genética , Linhagem Celular Tumoral , Proliferação de Células/genética , Proteínas Contráteis , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , MicroRNAs/genética , MicroRNAs/metabolismo , Paclitaxel/farmacologia , Paclitaxel/uso terapêutico , RNA Circular/genética
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