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1.
Curr Microbiol ; 2020 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-32382952

RESUMO

A Gram-stain-negative, aerobic bacterium, designated HXT-9T, was isolated from a river sediment. Cells were rod-shaped and non-motile. Growth occurred at 10-42 ℃ (optimum 30 ℃), at pH 5.0-8.0 (optimum pH 7.0) and with NaCl concentrations of 0-2.5% (optimum 0.5%). Phylogenetic trees based on 16S rRNA gene sequences showed that strain HXT-9T belonged to the genus Parvibaculum, and showed highest similarities to P. lavamentivorans KCTC 22775T (96.1%), followed by P. hydrocarboniclasticum EPR 92T (94.8%) and P. indicum P31T (93.6%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain HXT-9T and P. lavamentivorans KCTC 22775T were 75.2% and 20.8%, respectively. The G + C content of strain HXT-9T genome was 62.1 mol%. The major fatty acids (> 10%) were summed feature 8 (C18:1ω7c and/or C18:1ω6c), C19:0 cyclo ω8c and C16:0 3-OH. The major respiratory quinone was ubiquinone 11 (Q-11). The major polar lipids were DPG (diphosphatidylglycerol), PG (phosphatidylglycerol), PME (phosphatidylmonomethylethanolamine), PE (phosphatidylethanolamine), PC (phosphatidylcholine) and AL (unidentified aminolipids). The phylogenetic analysis and physiological and biochemical data showed that strain HXT-9T represents a novel species in the genus Parvibaculum, for which the name P. sedimenti sp. nov. is proposed. The type strain is HXT-9T (= CCTCC AB 2019273T = KCTC 72547T).

2.
Pestic Biochem Physiol ; 166: 104449, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32448412

RESUMO

Tobacco mosaic virus helicase (TMV-Hel) plays important roles in viral multiplication. TMV-Hel is a potential target of anti-TMV agents. Our previous studies expressed and purified TMV-Hel as target protein for cytosinpeptidemycin. In this study, we preform molecular docking to study the binding sites of commercial antiviral agents with TMV-Hel. Then we verify the interactions between the potential anti-TMV agents and TMV-Hel in vitro using Microscale Thermophoresis experiment and study the inhibiting expression of TMV-Hel with the potential anti-TMV agents in vivo using Western-blot (WB) method. The results showed that ribavirin bound to TMV-Hel with a dissociation constant of 1.55 µM by direct interaction with eight binding sites, which was consistent with the docking studies. Ribavirin inhibited the expression of TMV-Hel in Nicotiana benthamiana. Docking studies combined Microscale Thermophoresis and WB experiment provided a new method to screen anti-TMV agents targeting TMV-Hel.


Assuntos
Antivirais , Vírus do Mosaico do Tabaco , Simulação de Acoplamento Molecular , Tabaco
3.
Langmuir ; 2020 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-32281378

RESUMO

The synthesis of some complex polyoxometalates (POMs) is critical to develop potential photocatalysts with high catalytic activity and selectivity. Here, we address this challenge by a hydrothermal self-assembly route to obtain a novel POM-based Co4W6O21(OH)2·4H2O with a hierarchical microsphere structure. The Co4W6O21(OH)2·4H2O crystallizes in the cubic space group Im3̅ with cell parameters: a = b = c = 12.878 Å, α = ß = γ = 90°, and Z = 4. The structure is further characterized by X-ray diffraction, X-ray photoelectron spectroscopy, UV-vis spectroscopy, thermogravimetric analysis, and Fourier transform infrared spectra. After depositing Ag2O nanoparticles on the 3D Co4W6O21(OH)2·4H2O microsphere by photochemical synthesis, the Co4W6O21(OH)2·4H2O/Ag2O heterojunction presents enhanced photocatalytic activity for RhB compared with P25 and pristine Ag2O. Moreover, we confirm the key role of holes for the Co4W6O21(OH)2·4H2O/Ag2O and put forward a possible mechanism for the photocatalytic degradation reaction.

4.
J Comput Biol ; 2020 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-32191538

RESUMO

The classical methods for the classification problem include hypothesis test with the Benjamini-Hochberg method, hidden Markov chain model, and support vector machine. One major application of the classification problem is gene expression analysis, for example, detecting the host genes having interaction with pathogen. The classical methods can be applied and have a good performance when the number of genes having interaction with the pathogen is not sparse with respect to the candidate genes. However, conditional random field (CRF), with an appropriate design, can be applied and have good performance even when it is sparse. In this work, we proposed a modified CRF with a baseline to reduce the number of parameters in CRF. Moreover, we show an application of CRF with the least absolute shrinkage and selection operator (LASSO) to classifying barley genes of its reaction to the pathogen.

5.
J Sci Med Sport ; 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-32197926

RESUMO

OBJECTIVES: To assess the association between comprehensive physical fitness and high blood pressure (HBP) among Chinese children and adolescents. DESIGN: National cross-sectional surveys. METHODS: 214,301 school students' data aged 7-18years was extracted in 2014. Six components of physical fitness (forced vital capacity, standing long jump, sit-and-reach, body muscle strength, 50m dash and endurance running) were measured, standardized and aggregated as a summary physical fitness indicator (PFI). HBP, systolic HBP (SHBP) and diastolic HBP (DHBP) were defined according to sex-, age- and height-specific references in China. RESULTS: The prevalence of HBP, SHBP and DHBP was 8.6%, 4.7% and 5.7%, respectively, and PFI was -0.9 in Chinese children and adolescents. A significant negative association between the PFI and HBP was observed with adjusted prevalence of HBP (10.8% (95% CI: 10.4-11.2) to 7.6% (95% CI: 7.3-8.0), Ptrend<0.001), SHBP (5.7% (95% CI: 5.4-6.1) to 4.4% (95% CI: 4.1-4.6), Ptrend<0.001), and DHBP (7.6% (95% CI: 7.2-7.9) to 4.6% (95% CI: 4.3-4.9), Ptrend<0.001) and their ORs (HBP: 0.87(95% CI: 0.82-0.93) to 0.68(95% CI: 0.64-0.73), Ptrend<0.001; SHBP: 0.86(95% CI: 0.79-0.94) to 0.75(95% CI:0.69-0.82), Ptrend<0.001; DHBP: 0.85(95% CI: 0.79-0.92) to 0.59(95% CI: 0.54-0.64), Ptrend<0.001) declined with the increase in PFI. Stratified nutritional status exhibited a similar negative association between PFI and HBP, SHBP and DHBP in children with normal weight, overnutrition, and undernutrition. Stand long jump, body muscle strength, 50m dash, and endurance running, had a negative association with HBP, SHBP and DHBP, but forced vital capacity had a positive such association. Sit-and-reach and HBP are not significantly associated. CONCLUSIONS: Physical fitness was negatively correlated to the increased HBP in children and adolescents. Comprehensive policies and measures to enhance children and adolescents' physical fitness are urgently needed through the promotion of physical activity, healthy dietary patterns, and strategies of educational guidelines to reduce schoolwork, which will in turn reduce the cardiovascular burdens in the future.

6.
Nat Chem ; 12(4): 381-390, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32152477

RESUMO

Stimuli-responsive biomaterials that contain logic gates hold great potential for detecting and responding to pathological markers as part of clinical therapies. However, a major barrier is the lack of a generalized system that can be used to easily assemble different ligand-responsive units to form programmable nanodevices for advanced biocomputation. Here we develop a programmable polymer library by including responsive units in building blocks with similar structure and reactivity. Using these polymers, we have developed a series of smart nanocarriers with hierarchical structures containing logic gates linked to self-immolative motifs. Designed with disease biomarkers as inputs, our logic devices showed site-specific release of multiple therapeutics (including kinase inhibitors, drugs and short interfering RNA) in vitro and in vivo. We expect that this 'plug and play' platform will be expanded towards smart biomaterial engineering for therapeutic delivery, precision medicine, tissue engineering and stem cell therapy.

7.
Zhongguo Dang Dai Er Ke Za Zhi ; 22(2): 158-163, 2020 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-32051084

RESUMO

OBJECTIVE: To explore the effect of parental training based on the Early Start Denver Model (ESDM) combined with intensive training on the treatment outcome of children with autism spectrum disorder (ASD) and its impact on parenting stress. METHODS: Seventy children aged 2-5 years who were diagnosed with ASD were enrolled in the study. They were divided into an ESDM group and a parental training group by the random number table method (n=35 each). The ESDM group received intensive training based on ESDM. In addition to intensive ESDM-based training, parents of the children in the parental training group received ESDM skills training. Both groups were assessed by Autism Behavior Checklist (ABC), Childhood Autism Rating Scale (CARS), Autism Treatment Evaluation Checklist (ATEC) and Parenting Stress Index-Short Form (PSI-SF) before and after the intervention of 3 months. RESULTS: After 3 months of intervention, the total scores of ABC, CARS and ATEC were both significantly decreased in the two groups (P<0.05). There was no significant difference in the total scores of ABC, CARS and ATEC between the two groups before and after intervention (P>0.05). The change between ABC, CARS and ATEC total scores in the two groups had no significant difference (P>0.05). After 3 months of intervention, the total scores of PSI-SF were both significantly decreased in the two groups (P<0.05). The difficult child sub-scale scores in PSI-SF were significantly decreased in the ESDM group (P<0.05). While three sub-scale scores of parent distress, parent-child dysfunctional interaction and difficult child in PSI-SF were significantly decreased in the parental training group (P<0.05). Before and after intervention of 3 months, no significant difference was found in PSI-SF total scores between the two groups. Compared with the ESDM group, the change between PSI-SF total scores and two sub-scales of PSI-SF (parent distress and difficult child) were significantly bigger in the parental training group (P<0.05). CONCLUSIONS: Both the combination of intensive training and parent training based on ESDM and ESDM intensive training alone can improve the core symptoms of children with ASD aged 2-5 years and relieve the parenting stress, however, the former is more effective in relieving parenting stress.


Assuntos
Transtorno do Espectro Autista , Pré-Escolar , Humanos , Relações Pais-Filho , Poder Familiar , Pais , Estresse Psicológico
8.
Nat Commun ; 11(1): 183, 2020 01 10.
Artigo em Inglês | MEDLINE | ID: mdl-31924799

RESUMO

The basolateral amygdala (BLA) and ventral hippocampal CA1 (vCA1) are cellularly and functionally diverse along their anterior-posterior and superficial-deep axes. Here, we find that anterior BLA (aBLA) and posterior BLA (pBLA) innervate deep-layer calbindin1-negative (Calb1-) and superficial-layer calbindin1-positive neurons (Calb1+) in vCA1, respectively. Photostimulation of pBLA-vCA1 inputs has an anxiolytic effect in mice, promoting approach behaviours during conflict exploratory tasks. By contrast, stimulating aBLA-vCA1 inputs induces anxiety-like behaviour resulting in fewer approaches. During conflict stages of the elevated plus maze task vCA1Calb1+ neurons are preferentially activated at the open-to-closed arm transition, and photostimulation of vCA1Calb1+ neurons at decision-making zones promotes approach with fewer retreats. In the APP/PS1 mouse model of Alzheimer's disease, which shows anxiety-like behaviour, photostimulating the pBLA-vCA1Calb1+ circuit ameliorates the anxiety in a Calb1-dependent manner. These findings suggest the pBLA-vCA1Calb1+ circuit from heterogeneous BLA-vCA1 connections drives approach behaviour to reduce anxiety-like behaviour.


Assuntos
Ansiolíticos/farmacologia , Complexo Nuclear Basolateral da Amígdala/metabolismo , Região CA1 Hipocampal/metabolismo , Calbindina 1/metabolismo , Comportamento de Escolha/fisiologia , Doença de Alzheimer/metabolismo , Animais , Ansiedade , Comportamento Animal , Calbindina 1/genética , Tomada de Decisões , Masculino , Aprendizagem em Labirinto/fisiologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Neurônios/fisiologia , Proteômica
9.
Theriogenology ; 142: 421-432, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31711705

RESUMO

AMH (Anti-Müllerian Hormone) is involved in the regulation of follicle growth initiation and inhibits FSH-induced aromatase expression and estrogen production in granulosa cells. However, the function of AMH in steroidogenesis by theca cells remains unclear. The aim of this study is to investigate the role of AMH as a regulator of the basal and stimulated steroid production by pig granulosa cells (pGCs) and theca cells (pTCs). PGCs and pTCs were incubated with hormones AMH, LH (luteinizing hormone), FSH (follicle stimulating hormone), individually or in combination. The expression of CYP19A1, HSD3B1, CYP11A1, LHCGR, and CYP17A1 mRNA were evaluated by quantitative reverse transcriptase PCR. In pGCs, 10 ng/mL AMH significantly decreased the FSH-stimulated effect on FSHR and CYP19A1 expression and estradiol production. In pTCs, LH treatment significantly increased the expression of HSD3B1, CYP11A1, LHCGR, and androstenedione or progesterone production (P < 0.05). Additionally, 10 ng/mL AMH also significantly decreased the LH-stimulated effects on the expression of HSD3B1, CYP11A1, CYP17A1, LHCGR and androstenedione production. Transfection with siAMHR2-I abolished the suppressive effects of AMH on LH-induced HSD3B1 expression and androstenedione production. Taken together, these results demonstrate that AMH is involved in FSH induced estradiol production in pGCs and LH induced androstenedione production in pTCs by regulating the steroidogenesis pathway.

10.
Theriogenology ; 142: 158-168, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31593883

RESUMO

SIRT6, a member of the sirtuin family, is a NAD + dependent protein deacetylase and has been implicated in transcriptional regulation of somatic cells and post-transcriptional regulation of oocyte meiosis. However, the function of cumulus cell-derived and maternal SIRT6 in meiotic maturation of porcine oocytes is not yet known. Here, we report that SIRT6 mRNA and protein exists in the oocyte and its surrounding cumulus cells during meiotic maturation. Functional studies using a specific inhibitor in cumulus-enclosed oocytes revealed important roles for SIRT6 in germinal vesicle breakdown (GVBD) and cumulus expansion. Moreover, inhibitor treatment led to a significant reduction in the rate of first polar body (PB1) extrusion and early development of parthenogenetically activated embryos. In contrast, SIRT6 inhibition in cumulus-free oocytes only resulted in a significant reduction in the rate of PB1 extrusion. Furthermore, SIRT6 dysfunction regardless of the origin in both cumulus cells and oocytes severely impaired spindle organization and chromosome alignment at the metaphase stage. Molecularly, SIRT6 inhibition in cumulus cells significantly reduced expression of genes associated with cumulus expansion and gap junctional communication and even expression levels of active phosphorylated CDK1 in oocytes. Importantly, adenylate cyclase inhibition could partially rescue GVBD and PB1 extrusion in SIRT6-inhibited cumulus-enclosed oocytes. Taken together, these results demonstrate that cumulus cell-expressed and maternal SIRT6 differentially regulates porcine oocyte meiotic maturation.

11.
Cells ; 8(12)2019 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-31835702

RESUMO

The establishment of a functional trophectoderm (TE) epithelium is an essential prerequisite for blastocyst formation and placentation. Transcription coactivator yes-associated protein (YAP), a downstream effector of the hippo signaling pathway, is required for specification of both the TE and epiblast lineages in mice. However, the biological role of YAP in porcine blastocyst development is not known. Here, we report that maternally derived YAP protein is localized to both the cytoplasm and nuclei prior to the morula stage and is then predominantly localized to the TE nuclei in blastocysts. Functionally, maternal YAP knockdown severely impeded blastocyst formation and perturbed the allocation of the first two lineages. The treatment of embryos with verteporfin, a pharmacological inhibitor of YAP, faithfully recapitulated the phenotype observed in YAP deleted embryos. Mechanistically, we found that maternal YAP regulates multiple genes which are important for lineage commitment, tight junction assembly, and fluid accumulation. Consistent with the effects on tight junction gene expression, a permeability assay revealed that paracellular sealing was defective in the trophectoderm epithelium. Lastly, YAP knockdown in a single blastomere at the 2-cell stage revealed that the cellular progeny of the YAP+ blastomere were sufficient to sustain blastocyst formation via direct complementation of the defective trophectoderm epithelium. In summary, these findings demonstrate that maternal YAP facilitates porcine blastocyst development through transcriptional regulation of key genes that are essential for lineage commitment, tight junction assembly, and fluid accumulation.

12.
Nanoscale ; 11(48): 23296-23303, 2019 Dec 28.
Artigo em Inglês | MEDLINE | ID: mdl-31782483

RESUMO

The development of efficient and stable non-noble-metal electrocatalytic materials for the oxygen evolution reaction (OER) is a huge and important challenge at present. Herein, we report the prominent enhancement of OER activity via doping vanadium into CoFe-based layered double hydroxide (LDH) electrocatalysts. Electrochemical characterization shows that the Co2Fe0.5V0.5 LDH grown on carbon papers (CPs) has an enormous electrochemical surface area (ECSA) and exhibits the smallest overpotential of 242 mV at 10 mA cm-2, which only requires a small Tafel slope of 41.4 mV dec-1 in 1 M KOH solution. The X-ray photoelectron spectroscopy (XPS) peak position of Co, Fe and O moves slightly to higher binding energy, elucidating the improved covalency of the metal-oxygen bond after V doping. DFT+U simulation indicates that the outstanding electrocatalytic activity of Co2Fe0.5V0.5 could be ascribed to the increased metal-oxygen covalency in LDH after V element doping, and facilitates the charge-transfer from oxygen to the metal. This finely tuned strategy by V doping into the CoFe-based LDH matrix can adjust the covalency of metal-oxygen bridges and enhance its electrocatalytic activity for the OER. In this work, we also present a general method to study various highly efficient metal hydroxide catalysts for the OER.

13.
Org Biomol Chem ; 17(40): 9065-9069, 2019 10 28.
Artigo em Inglês | MEDLINE | ID: mdl-31584058

RESUMO

The efficient ruthenium-catalyzed meta-selective CAr-H nitration of azole ring substituted arenes has been developed. In this work, Ru3(CO)12 was used as the catalyst, AgNO2 as the nitro source, HPcy3+·BF4- as the ligand, pivalic acid as the additive, and DCE as the solvent, and a wide spectrum of arenes bearing thiazole, pyrazolyl or removable oxazoline directing groups were tolerated in this meta-selective CAr-H nitration, affording the nitrated products in moderate to good yields. Moreover, this study reveals a gentler and environmentally friendly way to access meta-nitration arenes compared to the traditional process.

14.
Aging (Albany NY) ; 11(18): 8015-8034, 2019 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-31562810

RESUMO

Thousands of circular RNAs (circRNAs) have been recently discovered in cumulus cells and oocytes from several species. However, the expression and function of circRNA during porcine oocyte meiotic maturation have been never examined. Here, we separately identified 7,067 and 637 circRNAs in both cumulus cells and oocytes via deep sequencing and bioinformatic analysis. Further analysis revealed that a faction of circRNAs is differentially expressed (DE) in a developmental stage-specific manner. The host genes of DE circRNAs are markedly enriched to multiple signaling pathways associated with cumulus cell function and oocyte maturation. Additionally, most DE circRNAs harbor several miRNA targets, suggesting that these DE circRNAs potentially act as miRNA sponge. Importantly, we found that maternal circARMC4 knockdown by siRNA microinjection caused a severely impaired chromosome alignment, and significantly inhibited first polar body extrusion and early embryo development. Taken together, these results demonstrate for the first time that circRNAs are abundantly and dynamically expressed in a developmental stage-specific manner in cumulus cells and oocytes, and maternally expressed circARMC4 is essential for porcine oocyte meiotic maturation and early embryo development.

15.
J Agric Food Chem ; 67(41): 11380-11387, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31535865

RESUMO

Southern rice black-streaked dwarf virus (SRBSDV) causes disease in crops, which reduces the quality and yield. Several commercial antiviral agents are available to control the SRBSDV induced disease. However, the mechanism of antiviral agents controlling SRBSDV is largely unknown. Identifying targets in SRBSDV is a key step of antiviral agent discovery. Here, we investigated the potential protein target of the antiviral agent dufulin. We cloned and expressed a soluble viroplasmic P6 protein in the prokaryote Escherichia coli and the eukaryote Spodoptera frugiperda 9. The dissociation constants of dufulin with the purified P6 protein from E. coli and S. frugiperda 9 expression systems were 4.49 and 4.95 µM, respectively, indicating a strong binding affinity between dufulin and P6 protein. In vivo, dufulin significantly inhibited the expression of both P6 protein and P6 gene in the SRBSDV-infected rice leaves. This inhibition on P6 protein expression was also observed in transformed Nicotiana benthamiana where the P6 was overexpressed. Our data also showed that dufulin inhibited the duplication of SRBSDV in a dose-dependent manner in infected rice leaves with a half maximum effective concentration of 3.32 mM. It is therefore concluded that dufulin targets the viroplasmic protein P6 to inhibit the virulence of SRBSDV.


Assuntos
Antivirais/farmacologia , Benzotiazóis/farmacologia , Doenças das Plantas/virologia , Reoviridae/efeitos dos fármacos , Proteínas Virais/metabolismo , Escherichia coli/genética , Escherichia coli/metabolismo , Regulação Viral da Expressão Gênica/efeitos dos fármacos , Oryza/virologia , Reoviridae/genética , Reoviridae/metabolismo , Proteínas Virais/genética
16.
Artigo em Inglês | MEDLINE | ID: mdl-31412652

RESUMO

OBJECTIVE: To investigate the relationship between low birthweight (LBW) and blood pressure and to assess whether LBW leads to a higher risk of high blood pressure (HBP) by gender in Chinese students aged 6-18 years. Also, to investigate whether the association was affected by childhood obesity. METHODS: Data was obtained from a baseline dataset of a national school-based program. Anthropometric parameters, including height, weight, and blood pressure, were measured, while birthweight and other characteristics were obtained from questionnaires. Stratified chi-squared tests were used to compare the prevalence of HBP between LBW and normal birthweight (NBW) groups in each age and sex category. Multivariable logistic regressions were conducted to estimate the HBP risks in each birthweight group. RESULTS: Both systolic and diastolic blood pressure showed a U-shaped relationship with increased birthweight. Compared to NBW groups, LBW girls showed a higher HBP risk, with an odds ratio of 1.29 (95% confidence interval (CI): 1.02, 1.64, p = 0.033), regardless of their current body mass index status, while no significant association in boys was found. Conclusions: Low birthweight is associated with higher HBP risk in adolescent girls, regardless of their childhood BMI status.


Assuntos
Hipertensão/epidemiologia , Recém-Nascido de Baixo Peso , Adolescente , Índice de Massa Corporal , Criança , China/epidemiologia , Estudos Transversais , Feminino , Humanos , Recém-Nascido , Razão de Chances , Medição de Risco
17.
BMC Infect Dis ; 19(1): 617, 2019 Jul 12.
Artigo em Inglês | MEDLINE | ID: mdl-31299910

RESUMO

BACKGROUND: The major infectious diseases of hepatitis B has constituted an acute public health challenge in China. An effective and affordable HBV control model is urgently needed. A national project of Community-based Collaborative Innovation HBV (CCI-HBV) demonstration areas has optimized the existing community healthcare resources and obtained initial results in HBV control. METHODS: Based on the existing community healthcare network, CCI-HBV project combined the community health management and health contract signing service for long-staying residents in hepatitis B screening. Moreover, HBV field research strategy was popularized in CCI-HBV areas. After screening, patients with seropositive results were enrolled in corresponding cohorts and received treatment at an early stage. And the uninfected people received medical supports including health education through new media, behavior intervention and HBV vaccinations. In this process, a cloud-based National Information Platform (NIP) was established to collect and store residents' epidemiological data. In addition, a special quality control team was set up for CCI project. RESULTS: After two rounds of screening, HBsAg positive rate dropped from 5.05% (with 5,173,003 people screened) to 4.57% (with 3,819,675 people screened), while the rate of new HBV infections was 0.28 per 100 person-years in the fixed cohorts of 2,584,322 people. The quality control team completed PPS sampling simultaneously and established the serum sample database with 2,800,000 serum samples for unified testing. CONCLUSIONS: CCI-HBV project has established a large-scale field research to conduct whole-population screening and intervention. We analyzed the HBsAg prevalence and new infection rate of HBV in the fixed population for the epidemic trend and intervention effect. The purpose of CCI-HBV project is to establish and evaluate a practical model of grid management and field strategy, to realize the new goal to control hepatitis B in China. To provide policymakers with a feasible model, our results are directly applicable. TRIAL REGISTRATION: The project was funded by the Major Projects of Science Research for the 11th and 12th five-year plans of China, entitled "The prevention and control of AIDS, viral hepatitis and other major infectious diseases", Grant Nos. 2009ZX10004901, 2011ZX10004901, 2013ZX10004904, 2014ZX10004007 and 2014ZX10004008.


Assuntos
Bases de Dados Factuais , Hepatite B/epidemiologia , Adolescente , China/epidemiologia , Computação em Nuvem , Serviços de Saúde Comunitária , Feminino , Política de Saúde , Hepatite B/diagnóstico , Hepatite B/prevenção & controle , Antígenos de Superfície da Hepatite B/sangue , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência
18.
Anal Chem ; 91(16): 10894-10900, 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31331163

RESUMO

Acrylate has been widely used as the recognition unit for Cys fluorescent probes. Despite this widespread use, a potential drawback of this probe type is that the ester linkage between the fluorophore and acryloyl recognition unit is liable to be hydrolyzed by abundant esterase in the cytosol, thus affording a high background signal. To solve this problem, we herein put forward a new strategy to construct a selective fluorescent probe for cysteine (Cys)/homocysteine (Hcy) with propynamide as the recognition moiety. The free probe CPA displays weakly fluorescent emission in aqueous media because of the donor-excited photoinduced electron transfer (d-PET) process within the molecule. The Michael addition of Cys (or Hcy) thiols to the conjugated alkyne of CPA gives the expected ß-sulfido-α,ß-unsaturated amides (1a/1b), which subsequently undergo an intramolecular S,N rearrangement, yielding ß-amino-α,ß-unsaturated amides (2a/2b) as the final products. The above cascade reaction results in the blockage of d-PET within CPA, thus affording a dramatic fluorescence enhancement at 495 nm. The involvement of the sulfhydryl and the adjacent amino groups in the sensing process renders CPA high selectivity for Cys/Hcy over glutathione as well as other amino acids. The probe has been successfully applied to image Cys in different cell lines. Further, CPA shows two-photon fluorescence properties, and its ability to monitor Cys in deep tissues has been demonstrated by using two-photon microscopy.

19.
Artigo em Inglês | MEDLINE | ID: mdl-31337125

RESUMO

OBJECTIVE: To investigate the association of long-term PM2.5 exposure with blood pressure (BP) outcomes in children aged 6-18 years, and to examine the population attributable risk (PAR) of PM2.5 exposure. METHODS: A total of 53,289 participants aged 6-18 years with full record of age, sex, BP, height, and local PM2.5 exposure from a cross-sectional survey conducted in 6 cities of China in 2013 were involved in the present study. PM2.5 data from 18 January 2013 to 31 December 2013 were obtained from the nearest environmental monitoring station for each selected school. Two-level linear and logistic regression models were used to evaluate the influence of PM2.5 on children's BP, and PAR was calculated in each sex and age group. RESULTS: Participants had a mean age of 10.8 (standard deviation: 3.4) years at enrollment, 51.7% of them were boys. U-shaped trends along with increased PM2.5 concentration were found for both systolic blood pressure (SBP) and diastolic blood pressure (DBP), with the thresholds of 57.8 and 65.0 µg/m3, respectively. Both increased annual mean of PM2.5 concentration and ratio of polluted days were associated with increased BP levels and high blood pressure (HBP), with effect estimates for BP ranging from 2.80 (95% CI: -0.51, 6.11) mmHg to 5.78 (95% CI: 2.32, 9.25) mmHg for SBP and from 0.77 (95% CI: -1.98, 3.52) mmHg to 2.66 (-0.35, 5.66) mmHg for DBP, and the odds ratios for HBP from 1.21 (0.43, 3.38) to 1.92 (0.65, 5.67) in the highest vs. the lowest quartiles. Overall, 1.16% of HBP in our participants could be attributed to increased annual mean of PM2.5 concentration, while 2.82% could be attributed to increased ratio of polluted days. These proportions increased with age. CONCLUSIONS: The association between long-term PM2.5 exposure and BP values appeared to be U-shaped in Chinese children aged 6-18 years, and increased PM2.5 exposure was associated with higher risk of HBP.


Assuntos
Exposição Ambiental , Hipertensão/epidemiologia , Material Particulado/análise , Instituições Acadêmicas , Adolescente , Pressão Sanguínea , Criança , Pré-Escolar , China/epidemiologia , Cidades , Estudos Transversais , Feminino , Humanos , Masculino , Razão de Chances
20.
BMJ Open ; 9(5): e024532, 2019 05 22.
Artigo em Inglês | MEDLINE | ID: mdl-31122966

RESUMO

BACKGROUND: The prevalence of childhood overweight and obesity in China has drastically increased 57 times over the past 30 years, and to control birth weight is an effective way to reduce the risk of overweight and obesity across the life course. OBJECTIVE: This paper aimed to evaluate the association of high birth weight (HBW) with overweight and obesity in Chinese students aged 6-18 years. METHODS: All students with HBW (n=4981) aged 6-18 years were selected from a cross-sectional survey from seven provinces of China, and 4981 other students with normal birth weight (NBW) were randomly sampled with matched gender, age and province. Anthropometric parameters were measured and characteristics were collected by questionnaires. Multiple logistic regression was used to estimate the OR of overweight and obesity with HBW, unadjusted and adjusted for confounding factors. RESULTS: Participants with HBW revealed higher body mass index in childhood. The prevalence of overweight and obesity was significantly higher in the HBW group than in the NBW group (overweight 15.3% vs 13.1%, p<0.05; obesity 16.9% vs 10.6%, p<0.05), and the results were similar for overweight in all age groups except age 6-7, age 14-15 and age 16-18. Additionally, HBW was positively associated with overweight (OR=1.230; 95% CI 1.056 to 1.432) and obesity (OR=1.611; 95% CI 1.368 to 1.897) after adjustment for covariates. CONCLUSIONS: HBW leads to an increased risk of overweight and obesity in childhood; thus, measures to control birth weight, such as controlling gestational weight gain, should be taken from the earliest beginning of life. TRIAL REGISTRATION NUMBER: NCT02343588; Post-results.

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