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1.
Enzyme Microb Technol ; 164: 110190, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36603321

RESUMO

Carotenoids are a series of natural pigments with unique structure and physiological functions. In this study, a novel Rhodococcus aetherivorans strain N1 was discovered, which can produce 6.4 mg/g carotenoids including ß-carotene, zeaxanthin and isorenieratene from glucose. Moreover, strain N1 can directly produce 3.0 mg/g carotenoids from the undetoxified straw hydrolysate, representing the highest carotenoids production from the undetoxified lignocellulosic hydrolysate. The crude carotenoid extracts of strain N1 showed efficient free radical scavenging activity and stability. Strain N1 has complete methylerythritol 4-phosphate (MEP) pathway and related genes for carotenoid synthesis, especially the rare aromatic carotenoid of isorenieratene. Genomic comparison between strain N1 and other carotenoid producing Rhodococcus sp. strains showed the conservatism and universality of carotenoids synthesis gene. These results proved that R. aetherivorans strain N1 can serve as a promising producer for the industrialization of carotenoid production.


Assuntos
Carotenoides , Rhodococcus , Carotenoides/metabolismo , Fenóis , Rhodococcus/genética , Rhodococcus/metabolismo
2.
Artigo em Inglês | MEDLINE | ID: mdl-36651706

RESUMO

Objective: The purpose of this study was to investigate the effect and influencing factors of postsurgical radioactive iodine (RAI) therapy for patients with low- and intermediate-risk differentiated thyroid cancer (DTC). Subjects and methods: A retrospective analysis of 423 low- and intermediaterisk DTC patients admitted to the Department of Nuclear Medicine, Sichuan Provincial People's Hospital from January 2005 to December 2020 was performed. All patients were treated with surgery, had a postoperative pathological diagnosis, and were treated with RAI, including 89 males and 334 females. Recurrence risk stratification: 143 cases were low-risk, and 280 cases were intermediaterisk. Results: The excellent response (ER) rate for low- and intermediate-risk were 93.7% and 78.2%, respectively (P < 0.05). There were significant differences in age, cumulative dose of [131I], and pretreatment stimulated-Tg (pre-Tg) levels between the low- and intermediate-risk groups (P < 0.05). There were significant differences in the cumulative dose of 131I and pre-Tg levels between ER and the non-ER group (P < 0.05). The area under the curve (AUC) values were 0.799 in the low-risk group, and 0.747 in the intermediate-risk group for the ROC curve by ER status of pre-Tg. The ER rate with RAI treatment decreased with an increase in pre-Tg levels. Conclusion: Pre-Tg was an important factor for RAI treatment decision-making and prognostic evaluation and differed between low-risk and intermediate-risk DTC. Aggressive RAI therapy was recommended for low-risk DTC with pre-Tg ≥ 20.0 ng/mL and in intermediate-risk group with pre-Tg ≥ 10.0 ng/mL.

3.
Food Chem ; 411: 135409, 2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36682168

RESUMO

Co-pigmentation effect of phenolic acids on cyanidin-3-O-glucoside (C3G) and the mechanisms were investigated. Sinapic acid (SIA), ferulic acid (FA), p-coumaric acid (p-CA) and syringic acid (SYA) significantly enhanced C3G stability (P < 0.05), whereas vanillic acid (VA) and gallic acid (GA) showed no influence (P > 0.05). Among these phenolic acids, SIA and FA had higher binding coefficient with C3G (48.83 and 43.38), reduced degradation rate constant by 40.0 âˆ¼ 50.0 %, prolonged half-life by 74.6 âˆ¼ 94.7 % at 323 K, and significantly inhibited C3G hydration reaction (pKh = 2.87 and 2.80, P < 0.05). Molecular docking revealed that C3G and co-pigments were connected by hydrogen bond and π-π stacking interaction. Hydroxycinnamic acids of SIA, FA and p-CA bound with ring B and ring C of C3G, while hydroxybenzoic acids of SYA, VA and GA hardly interacted with ring C. Generally, the protection effect of hydroxycinnamic acids on C3G was better than that of hydroxybenzoic acids, exhibiting stronger hyperchromic effect.

4.
Food Chem ; 408: 135191, 2023 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-36527919

RESUMO

Food quality, safety, and the regulatory metabolism of food nutrients in cells are primary factors in determining human health. However, residues of undesirable or hazardous compounds in food products and dysregulation in the nutrient metabolism inevitably occur occasionally. For years, chromatography-mass spectrometry technology has been recognized as an essential research tool in food analysis and nutrient metabolism research, and it is more accurate and robust when coupled with stable isotopes. In this study, we summarize the applications of stable isotope technology in the quantification of contaminant residues (pesticides, veterinary drugs, mycotoxins, polycyclic aromatic hydrocarbons, and other hazardous compounds) in foods and in the nutrients (glucose, lipids, amino acids and proteins) metabolism research. The aim of this review was to serve as a reference for providing effective analysis techniques for protecting food quality and human health, and to pave the way for the broader application of stable isotope technology.


Assuntos
Análise de Perigos e Pontos Críticos de Controle , Isótopos , Humanos , Isótopos/análise , Espectrometria de Massas/métodos , Proteínas/análise , Nutrientes/análise , Análise de Alimentos/métodos , Marcação por Isótopo/métodos
5.
Diabetes Metab Syndr Obes ; 15: 3705-3715, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36465992

RESUMO

Purpose: To evaluate the relationships of the triglyceride-glucose (TyG) index with pregnancy-related complications (PRCs) and to clarify the predictability of the TyG index for PRCs. Patients and Methods: Totally of 11,387 women with a singleton pregnancy were prospectively followed until after delivery. Maternal fasting lipids and glucose concentration were measured in the first trimester (11 weeks gestation on average). The TyG index was calculated as ln [triglyceride (mg/dL) × fasting plasma glucose (mg/dL)/2]. We used generalized linear models to calculate the relative risks and 95% confidence intervals. Receiver-operating characteristic curve analysis was employed to assess the ability of the TyG index to predict the risks of PRCs. Results: Smooth spline reveals that the probability of gestational diabetes mellitus (GDM) is intensified with the increasing TyG index. Multivariate logistic regression adjusted for risk factors demonstrates a 1-unit and a 1-SD increment in the TyG index raises the risk of GDM by 3.63 and 1.57 times, respectively. Identically, the risk of GDM maximizes in the TyG quintile 5 (OR: 3.14; 95% CI: 2.55~3.85) relative to the lowest TyG index group. However, no association between TyG index and the risk of other PRCs was observed after full adjustment. The area under receiver operating characteristic curves is 0.647 (95% CI: 0.632-0.66) for GDM, and the optimal predictive cut-off is 8.55, with a specificity of 0.679 and sensitivity of 0.535. Conclusion: The first-trimester TyG index is significantly associated with the risk of incident GDM, while the relationships between the TyG index and other PRCs need further exploration.

6.
Front Plant Sci ; 13: 1024909, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36388486

RESUMO

Fruit acidity is an essential factor affecting blueberry organoleptic quality. The organic acid content in blueberry fruit mainly contributes to fruit acidity. This study aims to evaluate the effect of exogenous salicylic acid (SA), the principal metabolite of aspirin, on the organoleptic quality and organic acid metabolism in rabbiteye blueberry (Vaccinium virgatum Ait, 'Powderblue') during cold storage (4 °C). Results showed that SA-treated fruit reduced fruit decay and weight loss delayed fruit softening, and decline of total soluble solids (TSS). TA and total organic acid amounts stayed the same during the late storage period in SA-treated fruit. Four kinds of organic acid components, malic acid, quinic acid, citric acid, and succinic acid, were at higher levels in fruit treated by SA as compared to control. SA enhanced the activities of PEPC, NAD-MDH, and CS to promote the synthesis of malic acid and citric acid. Meanwhile, the activities of NADP-ME, ACL, and ACO, which participated in the degradation of malic acid and citric acid, were inhibited by SA. qPCR results also showed that the expression of VcPEPC, VcNAD-MDH, and VcCS genes were upregulated. In contrast, SA downregulated the expression of VcNADP-ME, VcACL, and VcACO genes. In conclusion, SA could regulate the key genes and enzymes that participated in organic acids metabolism to maintain the freshness of blueberry during cold storage, therefore minimizing the economic loss.

7.
J Insect Sci ; 22(6)2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36374481

RESUMO

Oedaleus asiaticus (Bey-Bienko) is an economically devastating locust species found in grassland and pastoral areas of the Inner Mongolia region of northern China. In this study, resistance to three frequently used insecticides (beta-cypermethrin, matrine, and azadirachtin) was investigated in six field populations of O. asiaticus using the leaf-dip bioassay method. The inhibitory effects of synergists and the activities of detoxification enzyme activities in the different populations were determined to explore potential biochemical resistance mechanisms. The results showed that the field populations SB (resistance ratio [RR] = 7.85), ZB (RR = 5.64), and DB (RR = 6.75) had developed low levels of resistance to beta-cypermethrin compared with a susceptible control strain. Both the SB (RR = 5.92) and XC (RR = 6.38) populations had also developed low levels of resistance against matrine, with the other populations remaining susceptible to both beta-cypermethrin and matrine. All field populations were susceptible to azadirachtin. Synergism analysis showed that triphenyl phosphate (TPP) and diethyl-maleate (DEM) increased the toxicity of beta-cypermethrin significantly in the SB population, while the synergistic effects of TPP, piperonyl butoxide (PBO), and DEM on the toxicity of matrine were higher in SB (SR 3.86, 4.18, and 3.07, respectively) than in SS (SR 2.24, 2.86, and 2.29, respectively), but no synergistic effects of TPP, PBO, and DEM on azadirachtin were found. Biochemical assays showed that the activities of carboxylesterases (CarEs) and glutathione-S-transferases (GSTs) were significantly raised in all field populations of O. asiaticus, with a significant positive correlation observed between beta-cypermethrin resistance and CarE activity. The activities of cytochrome P450 monooxygenases (P450) and multi-function oxidases (MFO) were elevated in all six field populations, and P450 activity displayed strong positive correlations with the three insecticides. Our findings suggest that resistance to beta-cypermethrin in O. asiaticus may be mainly attributed to elevated CarE and GST activities, while P450 plays an important role in metabolizing matrine and azadirachtin. Our study provides insights that will help improve insecticide resistance management strategies.


Assuntos
Gafanhotos , Inseticidas , Piretrinas , Animais , Inseticidas/farmacologia , Piretrinas/farmacologia , Resistência a Inseticidas , China
8.
Nat Commun ; 13(1): 6773, 2022 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-36351933

RESUMO

DNA phosphorothioate (PT) modification, with a nonbridging phosphate oxygen substituted by sulfur, represents a widespread epigenetic marker in prokaryotes and provides protection against genetic parasites. In the PT-based defense system Ssp, SspABCD confers a single-stranded PT modification of host DNA in the 5'-CPSCA-3' motif and SspE impedes phage propagation. SspE relies on PT modification in host DNA to exert antiphage activity. Here, structural and biochemical analyses reveal that SspE is preferentially recruited to PT sites mediated by the joint action of its N-terminal domain (NTD) hydrophobic cavity and C-terminal domain (CTD) DNA binding region. PT recognition enlarges the GTP-binding pocket, thereby increasing GTP hydrolysis activity, which subsequently triggers a conformational switch of SspE from a closed to an open state. The closed-to-open transition promotes the dissociation of SspE from self PT-DNA and turns on the DNA nicking nuclease activity of CTD, enabling SspE to accomplish self-nonself discrimination and limit phage predation, even when only a small fraction of modifiable consensus sequences is PT-protected in a bacterial genome.


Assuntos
DNA , Genoma Bacteriano , DNA Bacteriano/genética , DNA/genética , DNA/química , Fosfatos/metabolismo , Guanosina Trifosfato
9.
Insects ; 13(11)2022 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-36354858

RESUMO

The high-density-dependent phase change from solitary to gregarious individuals in locusts is a typical example of phenotypic plasticity. However, the underlying molecular mechanism is not clear. In this study, first, Oedaleus asiaticus were treated with high-density population stress and then analyzed by Illumina sequencing on days 1, 3, 5, and 7 of the body color change to identify the stage-specific differentially expressed genes (DEGs). The KEGG pathway enrichment analysis of the identified DEGs revealed their role in metabolic pathways. Furthermore, the expression patterns of the nine key DEGs were studied in detail; this showed that the material change in locusts began on the third day of the high-density treatment, with the number of DEGs being the largest, indicating the importance of this period in the phase transition. In addition, the phenotypic change involved several key genes of important regulatory pathways, possibly working in a complex network. Phenotypic plasticity in locusts is multifactorial, involving multilevel material network interactions. This study improves the mechanistic understanding of phenotypic variation in insects at the genetic level.

10.
Int J Mol Sci ; 23(20)2022 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-36293335

RESUMO

Fruit softening that occurs during fruit ripening and postharvest storage determines the fruit quality, shelf life and commercial value and makes fruits more attractive for seed dispersal. In addition, over-softening results in fruit eventual decay, render fruit susceptible to invasion by opportunistic pathogens. Many studies have been conducted to reveal how fruit softens and how to control softening. However, softening is a complex and delicate life process, including physiological, biochemical and metabolic changes, which are closely related to each other and are affected by environmental conditions such as temperature, humidity and light. In this review, the current knowledge regarding fruit softening mechanisms is summarized from cell wall metabolism (cell wall structure changes and cell-wall-degrading enzymes), plant hormones (ETH, ABA, IAA and BR et al.), transcription factors (MADS-Box, AP2/ERF, NAC, MYB and BZR) and epigenetics (DNA methylation, histone demethylation and histone acetylation) and a diagram of the regulatory relationship between these factors is provided. It will provide reference for the cultivation of anti-softening fruits.


Assuntos
Frutas , Reguladores de Crescimento de Plantas , Frutas/metabolismo , Reguladores de Crescimento de Plantas/metabolismo , Histonas/metabolismo , Parede Celular/genética , Parede Celular/metabolismo , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/metabolismo
11.
J Sci Food Agric ; 2022 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-36254097

RESUMO

BACKGROUNDS: This research intended to explore the effects of different frozen storage temperatures and time on the stability of steamed bread. The quality characteristics, moisture content and microstructure of steamed bread were determined after quick-frozen for 30 min at -32 °C and frozen storage at -6, -12, -18, -24 and -30 °C for 1-4 weeks. RESULTS: When the frozen storage temperature is lower, the moisture content, specific volume, pH and the strong bound water in the steamed bread increase, the water loss rate and the contents of freezable water, the weak bound water and free water decreased. With the extension of frozen storage time, the pH value and water loss of steamed bread first increased and then decreased, while the trend of water content was opposite. The specific volume, cohesion and elasticity of steamed bread decreased, while the freezable water content, hardness and chewiness increased. The bound water of steamed bread gradually migrated to free water. In addition, the longer the frozen storage time and the higher the temperature, and the more serious the damage to the microstructure was. CONCLUSION: The shelf life of steamed bread frozen storage at -12 °C could be up to 3 weeks, and the quality of steamed bread stored at -30 °C for more than 3 weeks was the best. © 2022 Society of Chemical Industry.

12.
J Agric Food Chem ; 70(44): 14235-14246, 2022 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-36283033

RESUMO

The constituents of germinated brown rice (GBR), brown rice (BRR), and white rice (WHR) and their impact on metabolism, inflammation, and gut microbiota in high fat (HF) diet-fed mice were examined. The contents of total fiber and γ-aminobutyric acid in BRR and GBR were higher than that in WHR (p < 0.05). Male C57 BL/6J mice received HF diet+26 g% of WHR, BRR, or GBR for 12 weeks. BRR and GBR comparably reduced HF diet-induced increases in fasting plasma glucose, lipids, insulin resistance, and inflammatory markers compared to WHR (p < 0.01). The abundance of fecal Bacteroidetes in mice fed HF+GBR or HF+BRR was higher than in HF+WHR-fed mice (p < 0.05). The abundance of fecal Lactobacillus gasseri in GBR-fed mice was greater than that in WHR- or BRR-fed mice (p < 0.05). The results indicated that GBR or BRR attenuated hyperglycemia, insulin resistance, and inflammation in mice. HF+GBR, but not HF+BRR, increased a probiotic bacteria in the gut.


Assuntos
Microbioma Gastrointestinal , Resistência à Insulina , Oryza , Camundongos , Masculino , Animais , Dieta Hiperlipídica/efeitos adversos , Insulina , Inflamação , Camundongos Endogâmicos C57BL
13.
J Oncol ; 2022: 1892459, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36157236

RESUMO

The tumor suppressor protein B-cell translocation gene 2 (BTG2) is downexpressed in lung adenocarcinoma (LUAD); however, its role in LUAD survival remains unknown. This investigation is aimed at exploring the activity of BTG2 in LUAD. We analyzed BTG2 expression in LUAD datasets of the TCGA database and examined that BTG2 was markedly downregulated in comparison with adjacent normal tissues. The prognostic analysis suggested that higher expression of BTG2 protein correlates with prolonged survival in patients. Vectors expressing BTG2 were stably transduced into lung adenocarcinoma A549 cells. The overexpression of BTG2 in A549 cells causes cellular G1 phase arrest but did not affect cell proliferation, accompanied by increased activation of NF-κB. Our data indicate that BTG2 overexpression may trigger an autoregulatory prosurvival NF-κB pathway, which is resistant to environmental intervention owing to an increased level of BTG2.

14.
Int J Biol Macromol ; 221: 1093-1102, 2022 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-36113588

RESUMO

Chia seed gum (CSG) plays an important role in the aggregation and structural properties of gluten protein. The experimental results showed that adding 1.0 % CSG increased the freezing rate and shortened the freezing time by 42.3 % compared with gluten without CSG. At the same time, CSG had no significant effect on the composition of the gluten subunit but could better control the change in binding water and delay the structural deterioration caused by the extension of time (30 d). The viscoelasticity of gluten was increased, but only with the addition of 0.2-0.6 % CSG. In addition, it increased the denaturation transition temperature (Tp) and the degradation temperature (Td) of gluten protein to reduce the occurrence of recrystallization and resist pyrolysis. During frozen storage, gluten can form fine ice crystals and inhibit the transformation of α-helices and ß-turns to random coils and ß-sheets, which is more conducive to long-term frozen storage.


Assuntos
Glutens , Sementes , Glutens/química , Congelamento , Sementes/química , Viscosidade
15.
J Glob Health ; 12: 11010, 2022 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-35973040

RESUMO

Background: The effects of meteorological factors and air pollutants on respiratory diseases (RDs) were various in different populations according to the demographic characteristics, and children were considered a vulnerable population. Previous studies were mainly based in cities with serious air pollution. This study aimed to qualify the lag effects of meteorological factors and air pollution on respiratory diseases among children under 18 years old in Fuzhou. Methods: Meteorological data, air pollutants concentrations and hospital admission data of Fujian Maternity and Child Health Hospital between 2015 and 2019 were collected. A Distributed Lag Nonlinear Model (DLNM) was used to evaluate the nonlinear and lagged effect of meteorological factors and air pollutants on daily RDs number. A subgroup analysis was also conducted to evaluate the effect on different sex groups and age groups. Results: A total number of 796 125 RDs visits was included during the study period. For meteorological factors, lower mean temperature and relative humidity were significantly associated with daily RDs number (peak relative risk (RR) = 1.032 (95% confidence interval (CI) = 1.011-1.053) and 1.021 (95% CI = 1.013-1.029)), while lower wind speed showed a significant association at low range (peak RR = 0.995 (95% CI = 0.992-0.999)). Temperature warming was a significant protective factor for RDs (peak RR = 0.989 (95% CI = 0.986-0.993)). For air pollutants, SO2, NO2, PM10 and PM2.5 were all significantly associated with RDs (peak RR = 1.028 (95% CI = 1.022-1.035), 1.024 (95% CI = 1.013-1.034), 1.036 (95% CI = 1.025-1.047), 1.028 (95% CI = 1.019-1.037)), and the relationship had no threshold. The estimated RR and peak lag day did not change extremely between subgroups. Conclusions: The findings provide statistical evidence for the prevention of child RDs. In addition, our findings suggested that even at low concentrations, air pollutants still have negative effects on the respiratory system.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Transtornos Respiratórios , Adolescente , Poluentes Atmosféricos/efeitos adversos , Poluentes Atmosféricos/análise , Poluição do Ar/efeitos adversos , Criança , China/epidemiologia , Feminino , Humanos , Conceitos Meteorológicos , Gravidez
16.
Acta Haematol ; 145(6): 627-641, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35933981

RESUMO

INTRODUCTION: The aim of the study was to construct a pyroptosis-related risk score (RS) model for the prognosis of acute myeloid leukemia (AML). METHODS: The TARGET (training) and E-MTAB-1216 (validation) datasets were downloaded. Pyroptosis-related genes with differences in expression were identified between the recurrent and nonrecurrent samples of the training dataset. An RS prognostic model comprising seven pyroptosis-related genes was constructed using LASSO regression coefficients. The samples were classified into the high- and low-risk groups using the RS model; the differentially expressed genes (DEGs) between these groups were identified, followed by DEG functional analysis and the immunological evaluation of these groups. RESULTS: Forty-nine pyroptosis-related genes, including 22 DEGs, were screened. WT1, NPM, FLT3/ITD, and CEBPA mutations were found in most pediatric AML samples. An RS prognostic model was constructed using 7 pyroptosis-related genes. The two risk groups and prognostic data were significantly related. FLT3/ITD mutations, CEBPA mutations, and RS model status were identified as independent prognostic factors, using the clinical information. The DEGs between the two groups were correlated with immune-related pathways. Moreover, the immune cell distribution and the occurrence of immune-related pathways were notably decreased in the high-risk group. DISCUSSION/CONCLUSION: Seven pyroptosis-related genes, CHMP2A, PRKACA, CASP9, IRF2, CHMP3, HMGB1, and AIM2, can predict the prognosis and recurrence of childhood AML.


Assuntos
Leucemia Mieloide Aguda , Piroptose , Criança , Humanos , Leucemia Mieloide Aguda/diagnóstico , Leucemia Mieloide Aguda/genética , Leucemia Mieloide Aguda/epidemiologia , Mutação , Prognóstico , Piroptose/genética , Fatores de Risco
17.
J Psychosom Res ; 160: 110959, 2022 09.
Artigo em Inglês | MEDLINE | ID: mdl-35810504

RESUMO

BACKGROUND: The COVID-19 pandemic has had a significant impact on individuals' emotional wellbeing and mental health. However, little research has examined emotional resilience during the pandemic. This study investigated the changes in emotional distress among residents in Hubei, the epicenter of the pandemic in China during the early stage of the pandemic, and we examined the sociodemographic differences in their emotional recovery. METHODS: We undertook a two-wave panel survey of 3816 residents aged ≥18 in Hubei, China. The baseline survey was conducted during early February 2020, the peak of the outbreak. The follow-up survey was carried out when the pandemic was mainly under control. The data enabled us to investigate the within-person changes in COVID-19-related negative emotions. Mixed-effect regression models with a random effect for participants were used to accommodate repeated measures. RESULTS: Respondents reported high levels of emotional distress at the peak of the pandemic and experienced a decline in emotional distress when the pandemic was under control. Moreover, respondents aged 35-49, with a college education or above, were employed, and having better self-rated health experienced a more substantial decrease in negative emotions during the COVID-19 pandemic. CONCLUSION: This study identified vulnerable populations who may experience prolonged emotional distress during the COVID-19 pandemic in China. The results suggest that respondents who aged over 50, with no college education, were not employed, and with worse self-rated health were less resilient during the COVID-19 pandemic in China.


Assuntos
COVID-19 , Angústia Psicológica , COVID-19/epidemiologia , China/epidemiologia , Emoções , Humanos , Pessoa de Meia-Idade , Pandemias , SARS-CoV-2
18.
PLoS One ; 17(6): e0269237, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35727743

RESUMO

BACKGROUND: While restriction measures are critical in containing the COVID-19 outbreak, limited studies have investigated the behavioral and psychological impact of these measures. This study aimed to investigate the effects of physical and sedentary behavioral changes and online behavior during the COVID-19 pandemic on mental health and life satisfaction among the Chinese population. METHODS: The data were obtained from a cross-sectional survey of 2145 residents aged between 18 and 80 in Hubei province, China between March 23, 2020, and April 9, 2020. RESULTS: Participants who had high frequencies of physical activities before or during the COVID-19 outbreak exhibited higher levels of life satisfaction. Participants who increased their sitting time during the pandemic or kept sitting for more than eight hours before and during the pandemic reported worse mental health than those who maintained less sedentary behavior. Besides, participants who used the Internet for information seeking, communication, and entertainment more frequently reported better mental health and life satisfaction. In contrast, there was a positive association between commercial use of the Internet and symptoms of mental disorders. CONCLUSION: Given the link between physical and sedentary behavioral changes with worse mental wellbeing, strategies to reduce sedentariness and increase physical activity during the COVID-19 pandemic are needed.


Assuntos
COVID-19 , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , COVID-19/epidemiologia , China/epidemiologia , Estudos Transversais , Humanos , Saúde Mental , Pessoa de Meia-Idade , Pandemias , Satisfação Pessoal , SARS-CoV-2 , Comportamento Sedentário , Adulto Jovem
19.
Biotechnol Adv ; 60: 108004, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-35690272

RESUMO

Lignin represents the most abundant renewable aromatics in nature, which has complicated and heterogeneous structure. The rapid development of biotransformation technology has brought new opportunities to achieve the complete lignin valorization. Especially, Rhodococcus sp. possesses excellent capabilities to metabolize aromatic hydrocarbons degraded from lignin. Furthermore, it can convert these toxic compounds into high value added bioproducts, such as microbial lipids, polyhydroxyalkanoate and carotenoid et al. Accordingly, this review will discuss the potentials of Rhodococcus sp. as a cell factory for lignin biotransformation, including phenol tolerance, lignin depolymerization and lignin-derived aromatic hydrocarbon metabolism. The detailed metabolic mechanism for lignin biotransformation and bioproducts spectrum of Rhodococcus sp. will be comprehensively discussed. The available molecular tools for the conversion of lignin by Rhodococcus sp. will be reviewed, and the possible direction for lignin biotransformation in the future will also be proposed.


Assuntos
Hidrocarbonetos Aromáticos , Poli-Hidroxialcanoatos , Rhodococcus , Carotenoides/metabolismo , Hidrocarbonetos Aromáticos/metabolismo , Lignina/química , Fenóis , Poli-Hidroxialcanoatos/metabolismo , Rhodococcus/genética , Rhodococcus/metabolismo
20.
Phys Med Biol ; 67(15)2022 07 19.
Artigo em Inglês | MEDLINE | ID: mdl-35767979

RESUMO

Objective. Glioma is one of the most fatal cancers in the world which has been divided into low grade glioma (LGG) and high grade glioma (HGG), and its image grading has become a hot topic of contemporary research. Magnetic resonance imaging (MRI) is a vital diagnostic tool for brain tumor detection, analysis, and surgical planning. Accurate and automatic glioma grading is crucial for speeding up diagnosis and treatment planning. Aiming at the problems of (1) large number of parameters, (2) complex calculation, and (3) poor speed of the current glioma grading algorithms based on deep learning, this paper proposes a lightweight 3D UNet deep learning framework, which can improve classification accuracy in comparison with the existing methods.Approach. To improve efficiency while maintaining accuracy, existing 3D UNet has been excluded, and depthwise separable convolution has been applied to 3D convolution to reduce the number of network parameters. The weight of parameters on the basis of space and channel compression & excitation module has been strengthened to improve the model in the feature map, reduce the weight of redundant parameters, and strengthen the performance of the model.Main results. A total of 560 patients with glioma were retrospectively reviewed. All patients underwent MRI before surgery. The experiments were carried out on T1w, T2w, fluid attenuated inversion recovery, and CET1w images. Additionally, a way of marking tumor area by cube bounding box is presented which has no significant difference in model performance with the manually drawn ground truth. Evaluated on test datasets using the proposed model has shown good results (with accuracy of 89.29%).Significance. This work serves to achieve LGG/HGG grading by simple, effective, and non-invasive diagnostic approaches to provide diagnostic suggestions for clinical usage, thereby facilitating hasten treatment decisions.


Assuntos
Neoplasias Encefálicas , Glioma , Neoplasias Encefálicas/patologia , Glioma/diagnóstico por imagem , Glioma/patologia , Humanos , Imageamento por Ressonância Magnética/métodos , Gradação de Tumores , Estudos Retrospectivos
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