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1.
Zhongguo Zhong Yao Za Zhi ; 45(15): 3651-3658, 2020 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-32893554

RESUMO

As an important substitute for agarwood, mountain-agarwood, belonging to the family Oleaceae, comes from the root, stem and thick branch of Syringa pinnatifolia, which has a wide range of application in Inner Mongolia, China. It has good clinical efficacy in the use of cardiovascular diseases. However, the formation speed of mountain-agarwood is extremely slow, and its cultivated seedlings have low resin content. Therefore, how to speed up the formation of mountain-agarwood and increase the resin content is a hot research topic in this field. In this work, 16 S rDNA amplicon sequencing method was used to systematically analyze the bacterial communities of different samples of mountain-agarwood. Our data revealed that the samples of mountain-agarwood had more obvious species diversity than the ones of non-mountain-agarwood, especially the wild mountain-agarwood samples. By analysis of bacterial community composition and species abundance, Sphingomonas, Modestobacter and unidentified Cyanobacteria genus were three dominant bacterial genera in all samples. In addition, there are two identified genera of dominant bacteria, namely Actinoplanes and Microbacterium in both wild and cultivated mountain-agarwood, by bacterial community composition and species richness analysis. Meanwhile, Roseomonas was the dominant bacterial genus in both wild and cultivated non-mountain-agarwood samples. Our work could provides basic data for exploring the mechanism of the mountain-agarwood formation, and help to exploit resource of endophytic bacteria reasonably.


Assuntos
Thymelaeaceae , Bactérias/genética , China , DNA Ribossômico , Resinas Vegetais
2.
Zhongguo Zhong Yao Za Zhi ; 45(16): 3797-3804, 2020 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-32893573

RESUMO

Mountain-agarwood plays an important role in ethnic medicine in China for its pharmaceutical value. Modern pharmacological researches demonstrated that mountain-agarwood was effective for its anti-myocardial ischemia, antibacterial, anti-inflammatory, antitumor and analgesic effects. Mountain-agarwood derives from the peeled roots, stems or twigs of Syringa pinnatifolia which belongs to Syringa genus. It often depends on the purple substance and fragrance to estimate the formation of mountain-agarwood. However, the mechanism of mountain-agarwood formation has not been reported. To observe the microcosmic change in the process during the formation of mountain-agarwood, this study described the microscopic and histochemical characteristics of mountain-agarwood formation through histochemical staining. Our results showed that a significant difference of the distribution of tyloses existed during mountain-agarwood formation. It was observed that inchoate mountain-agarwood had more starch granules and viable cells than mountain-agarwood formed with high level or low level. The amount of polysaccharide and degree of lignification were increased during the mountain-agarwood formation. The results indicated that the mountain-agarwood, which meets the quality requirements for pharmaceutical use, contained the following characteristics: a large amount of purple tyloses in heartwood; yellow-brown tyloses distributing in heartwood and sapwood which were less in the latter; lignification with high level; a few viable cells; lots of polysaccharide and few starch granules in xylem rays cell. This study is aimed to reveal the change of histochemical characteristics during mountain-agarwood formation, and lay the foundation for exploring the mechanism of mountain-agarwood formation.


Assuntos
Isquemia Miocárdica , Syringa , Thymelaeaceae , China , Humanos
3.
Zhongguo Zhong Yao Za Zhi ; 45(10): 2343-2352, 2020 May.
Artigo em Chinês | MEDLINE | ID: mdl-32495591

RESUMO

Syringa plants are of important value in ornamental, economic and medical fields. The terpenoids in Syringa plants mainly include iridoids, sesquiterpenoids, and triterpenoids, most showing activities such as cardioprotective, neuroprotective, hypoglycemic, anti-flu virus, anti-bacterial, anti-inflammatory, and anti-oxidation effects. Among the above active compounds, sesquiterpenoids have attracted increasing attention. In this review, the phytochemical and pharmacological activities of Syringa terpenoids were summarized in order to provide an overview for further research and development of Syringa plants.


Assuntos
Sesquiterpenos , Syringa , Triterpenos , Compostos Fitoquímicos , Terpenos
4.
Zhongguo Zhong Yao Za Zhi ; 45(10): 2374-2381, 2020 May.
Artigo em Chinês | MEDLINE | ID: mdl-32495595

RESUMO

To explore the diversity of bacterial community structure between different layers of agarwood, Hiseq(high-throughput sequencing) was used to analyze the bacterial community structure of samples from different layers of agarwood. Our results showed that 1 150 096 optimized sequences and 9 690 OTUs were obtained from 15 samples of 5 layers of agarwood, which belonged to 28 bacterial phyla, 61 classes, 110 orders, 212 families and 384 genera. Further analysis revealed that the normal layer(NL) had the lowest bacterial species richness and the smallest number of OTUs. And the total number of OTUs of the agarwood layer(AL) and NL was zero, which was quite different.At the same time, there were significant differences in bacterial community structure and species diversity between NL and the other four layers. While there were some common dominant bacterial genera in both transition layer(TL) and NL. The similarity of bacterial distribution in 4 non-NL layers was relatively high, which had four common genera, such as Acidibacter, Bradyrhizobium, Acidothemus and Sphingomonas. While Acidibacter, Bradyrhizobium and Acidothemus were the dominant bacterial genus of DA and AL, and all of these layers contained volatile oil. In addition, the Bradyrhizobium was the most abundant in agarwood layer. Our results showed that bacterial community diversity and abundance were decreasing from DL to AL, and different layers showed significant differences in bacterial enrichment. It provided the clues to investigate how bacteria participate in the formation of agarwood.


Assuntos
Óleos Voláteis , Thymelaeaceae/genética , Bactérias , Sequenciamento de Nucleotídeos em Larga Escala
5.
Huan Jing Ke Xue ; 40(11): 5066-5072, 2019 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854575

RESUMO

Substrate exposure levels are vital for the growth and metabolism of ANAMMOX microorganisms, and their effects on growth characteristics of ANAMMOX sludge during the enrichment process have been rarely reported. Using two continuous flow stirred reactors and the process of a gradually developing nitrogen load, the changes in biomass and activity, as well as nitrogen removal efficiency of the reactors were investigated under high substrate exposure level culture mode (R1:effluent NH4+-N and NO2--N concentrations were 40-60 mg·L-1) and low substrate exposure level culture mode (R2:effluent NH4+-N and NO2--N concentrations were 0-20 mg·L-1). The results showed that the high substrate exposure level culture mode was more beneficial to the improvement of nitrogen removal performance of the ANAMMOX reactor. For comparison, the NLR (nitrogen load rate), which was 0.69 kg·(m3·d)-1, and the NRR (nitrogen remove rate), which was up to 0.41 kg·(m3·d)-1, was obtained in the high substrate exposure culture mode. These values were twice as high as those obtained in the low substrate exposure culture mode. Under the culture mode with high substrate exposure level, the sludge concentration (in VSS) and the total gene copy numbers of ANAMMOX reached 1805 mg·L-1 and 4.81×1012 copies, respectively, which was conducive to the rapid enrichment of ANAMMOX microorganisms. In the low substrate exposure level culture mode, ANAMMOX sludge was more active,in N/VSS, 0.27 g·(g·d)-1, which was conducive to the cultivation of ANAMMOX sludge with higher biological activity.


Assuntos
Reatores Biológicos , Esgotos , Anaerobiose , Biomassa , Nitrogênio , Oxirredução
6.
Sci Rep ; 8(1): 6731, 2018 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-29695821

RESUMO

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.

7.
Chin Med J (Engl) ; 131(6): 713-720, 2018 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-29521295

RESUMO

Background:: Laparoscopic total gastrectomy (LTG) is increasingly performed in patients with gastric cancer. However, the usage of intracorporeal esophagojejunostomy (IEJ) following LTG is limited, as the safety and efficacy remain unclear. The present meta-analysis aimed to evaluate the feasibility and safety of IEJ following LTG. Methods:: Studies published from January 1994 to January 2017 comparing the outcomes of IEJ and extracorporeal esophagojejunostomy (EEJ) following LTG were reviewed and collected from the PubMed, EBSCO, Cochrane Library, Embase, and China National Knowledge Internet (CNKI). Operative results, postoperative recovery, and postoperative complications were compared and analyzed. The weighted mean difference (WMD) and odds ratio (OR) with a 95% confidence interval (CI) were calculated using the Review Manager 5.3. Results:: Seven nonrandomized studies with 785 patients were included. Compared with EEJ, IEJ has less blood loss (WMD: -13.52 ml; 95% CI: -24.82--2.22; P = 0.02), earlier time to first oral intake (WMD: -0.49 day; 95% CI: -0.83--0.14; P < 0.01), and shorter length of hospitalization (WMD: -0.62 day; 95% CI: -1.08--0.16; P < 0.01). There was no significant difference between IEJ and EEJ regarding the operation time, anastomotic time, number of retrieved lymph nodes, time to first flatus, anastomosis leakage rate, anastomosis stenosis rate, and proximal resections (all P > 0.05). Conclusions: Compared with EEJ, IEJ has better cosmesis, milder surgical trauma, and a faster postoperative recovery. IEJ can be performed as safely as EEJ. IEJ should be encouraged to surgeons with sufficient expertise.


Assuntos
Gastrectomia/métodos , Laparoscopia/métodos , Neoplasias Gástricas/cirurgia , Esofagostomia/efeitos adversos , Esofagostomia/métodos , Esôfago/cirurgia , Gastrectomia/efeitos adversos , Humanos , Jejunostomia/efeitos adversos , Jejunostomia/métodos , Laparoscopia/efeitos adversos , Resultado do Tratamento
8.
Sci Rep ; 7(1): 11095, 2017 09 11.
Artigo em Inglês | MEDLINE | ID: mdl-28894216

RESUMO

Surgeons' mental and physical workloads are major focuses of operating room (OR) ergonomics, and studies on this topic have generally focused on either mental workload or physical workload, ignoring the interaction between them. Previous studies have shown that physically demanding work may affect mental performance and may be accompanied by impaired mental processing and decreased performance. In this study, 14 participants were recruited to perform laparoscopic cholecystectomy (LC) procedures in a virtual simulator. Surface electromyography (sEMG) signals of the bilateral trapezius, bicipital, brachioradialis and flexor carpi ulnaris (FCU) muscles and eye-tracking signals were acquired during the experiment. The results showed that the least square means of muscle activity during the LC phases of surgery in an all-participants mixed effects model were 0.79, 0.81, and 0.98, respectively. The observed muscle activities in the different phases exhibited some similarity, while marked differences were found between the forearm bilateral muscles. Regarding mental workload, significant differences were observed in pupil dilation between the three phases of laparoscopic surgery. The mental and physical workloads of laparoscopic surgeons do not appear to be generally correlated, although a few significant negative correlations were found. This result further indicates that mental fatigue does markedly interfere with surgeons' operating movements.


Assuntos
Eletromiografia , Movimentos Oculares , Cirurgiões/psicologia , Carga de Trabalho , Adulto , Humanos , Laparoscopia , Masculino , Músculo Esquelético/fisiologia , Pupila/fisiologia
9.
Int J Clin Exp Pathol ; 10(9): 9704-9709, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-31966852

RESUMO

BACKGROUND: Totally laparoscopic distal gastrectomy gained wide popularity in recent years. Laparoscopic total gastrectomy with intracorporeal esophagojejunostomy (LTGIE) is much less performed. In this study, we reported our preliminary experience of LTGIE using the transorally inserted anvil (OrVil). METHODS: Clinical data of patients with upper gastric cancer who underwent LTGIE from January 2016 to January 2017 were retrospectively collected. The operative time, intraoperative blood loss, postoperative recovery time of intestinal function, the length of hospitalization and postoperative complications were summarized and compared between early and later cases. RESULTS: There were totally 26 patients underwent LTGIE using OrVil successfully. The mean total operation time and esophagojejunostomy time was 272.8 min and 45.3 min. The mean estimated blood loss was 113.8 ml. The mean first flatus time was 3.1±0.9 days and the postoperative length of hospitalization (LOH) was 13.0±6.4 days. Three patients suffered postoperative complications, including one abdominal fluid collection, one pulmonary embolism and one pulmonary infection. During the follow-up period, neither local recurrence nor anastomosis-related morbidity was observed. CONCLUSIONS: The LTGIE using OrVil is feasible and safe for upper gastric cancer. These preliminary results warrant further evaluation in a larger population to validate.

10.
Int J Oncol ; 49(2): 611-22, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27279633

RESUMO

Enhanced aldehyde dehydrogenase (ALDH) activity has been shown to serve as a hallmark for cancer stem cells (CSCs). Recent evidence suggests that its role as a stem cell-related marker has come down to the specific isoform. However, little is known about the specific ALDH isoform contributing to aldefluor activity in gastric cancer. In this study, we isolated ALDHbright cells from 2 human gastric cancer cell lines MKN-45 and SGC­7901 by using an Aldefluor assay and found elevated self-renewal, differentiation and tumorigenicity, as demonstration of stemness characteristics. We also found that ALDHbright cells expressed decreased levels of E-cadherin but increased levels of Snail and Vimentin, indication of an epithelial-mesenchymal transition (EMT) phenotype which may be responsible for the enhanced metastatic potential. Since further research and prognostic application based on ALDH prevalence require the quantification of the specific ALDH isoform, we characterized the expression of all 19 ALDH isoforms in the sorted gastric cancer cell lines by quantitative real-time polymerase chain reaction (qRT-PCR). Compared with the non-stem counterparts, robust upregulation of ALDH-3A1 was observed in these gastric cancer stem-like cells. Furthermore, we performed immunohistological analysis on 93 fixed patient gastric tumor samples and found that ALDH-3A1 expression correlated well with gastric cancer dysplasia and grades, differentiation, lymph node metastasis and cancer stage. Our data, therefore, provide strong evidence that ALDH-3A1 is a novel gastric cancer stem cell related marker with potential prognostic values and demonstrate a clear association between ALDH-3A1 prevalence and gastric cancer progression.


Assuntos
Aldeído Desidrogenase/metabolismo , Células-Tronco Neoplásicas/enzimologia , Neoplasias Gástricas/enzimologia , Aldeído Desidrogenase/biossíntese , Animais , Carcinogênese , Linhagem Celular Tumoral , Progressão da Doença , Feminino , Xenoenxertos , Humanos , Neoplasias Pulmonares/secundário , Masculino , Camundongos , Camundongos Endogâmicos NOD , Camundongos SCID , Pessoa de Meia-Idade , Células-Tronco Neoplásicas/patologia , Neoplasias Gástricas/patologia , Regulação para Cima
11.
Huan Jing Ke Xue ; 33(6): 1952-7, 2012 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-22946181

RESUMO

In this study, PM2.5 samples had been measured from the summer of 2009 to the winter of 2010 in a suburb of Shenzhen. The results indicated serious PM2.5 pollution in the suburb of Shenzhen. Seasonal and daily variations of water-soluble ions were discussed. SO4(2-), NO3(-) and NH4+ were the most abundant ions in winter. In summer, the average contributions of Cl(-), Na+, K+, Ca2+ and Mg2+ increased significantly, while SO4(2-), NO3(-) and NH4+ decreased. Furthermore, Naphthalene, acenaphthylene, acenaphthene and benzo fluoranthene were the major PAHs in PM2.5. Ratio analysis and characteristic compounds analysis were also used to determine the possible sources of PM2.5 in the suburb of Shenzhen.


Assuntos
Atmosfera/análise , Monitoramento Ambiental/métodos , Material Particulado , China , Cidades , Material Particulado/análise , Material Particulado/química , Hidrocarbonetos Policíclicos Aromáticos/análise
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