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1.
Rev Sci Instrum ; 92(9): 093904, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34598494

RESUMO

A novel high-resolution x-ray spectrometer for point-like emission sources has been developed using a crystal shape having both a variable major and a variable minor radius of curvature. This variable-radii sinusoidal spiral spectrometer (VR-Spiral) allows three common spectrometer design goals to be achieved simultaneously: 1. reduction of aberrations and improved spectral (energy) resolution, 2. reduction of source size broadening, and 3. use of large crystals to improve total throughput. The VR-Spiral concept and its application to practical spectrometer design are described in detail. This concept is then used to design a spectrometer for an extreme extended x-ray absorption fine structure experiment at the National Ignition Facility looking at the Pb L3 absorption edge at 13.0352 keV. The expected performance of this VR-Spiral spectrometer, both in terms of energy resolution and spatial resolution, is evaluated through the use of a newly developed raytracing tool, xicsrt. Finally, the expected performance of the VR-Spiral concept is compared to that of spectrometers based on conventional toroidal and variable-radii toroidal crystal geometries showing a greatly improved energy resolution.

2.
Int J Radiat Oncol Biol Phys ; 111(3S): e32-e33, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34701173

RESUMO

PURPOSE/OBJECTIVE(S): A reliable model is needed to estimate the risk of postoperative recurrence and the benefits of postoperative radiotherapy (PORT) in patients with thoracic esophageal squamous cell cancer (TESCC). MATERIALS/METHODS: The study retrospectively reviewed 3652 TESCC patients in stage I-III after radical esophagectomy, with or without PORT. In one institution as the training cohort (n = 1620), independent risk factors associated with locoregional recurrence (LRR), identified by the competing risk regression, were used to establish a predicting nomogram, which was validated in an external cohort (n = 1048). Area under curve (AUC) values of receiver operating characteristic curves were calculated to evaluate discrimination. Risk stratification was conducted using a decision tree analysis based on the cumulative point score of the LRR nomogram, and the effect of PORT was evaluated in each risk group. RESULTS: Sex, age, tumor location, tumor grade, and N category were identified as independent risk factors for LRR and added into the nomogram. The AUC values were 0.638 and 0.706 in the training and validation cohorts, respectively. Three risk groups were established. For patients in the intermediate- and high-risk groups, PORT significantly improved the 5-year overall survival by 8.8% and 11.1%, respectively (P < 0.05). Though PORT was significantly associated with reduced LRR in the low-risk group, overall survival was not improved. CONCLUSION: The nomogram can effectively estimate the individual risk of LRR, and patients in the intermediate- and high-risk groups are highly recommended to undergo PORT.

3.
Int J Radiat Oncol Biol Phys ; 111(3S): e33, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34701197

RESUMO

PURPOSE/OBJECTIVE(S): To compare the prognostic predictive power of the 11th Japan Esophageal Society (JES) system with the 8th American Joint Committee on Cancer (AJCC) system in patients with thoracic esophageal squamous cell carcinoma (TESCC), and to estimate the survival benefits of postoperative radiotherapy (PORT) based on substage of the JES system. MATERIALS/METHODS: Area under the curve (AUC) values of receiver operating characteristic curve were calculated to evaluate prognostic efficacy. Propensity score matching (PSM) analysis was conducted to balance the two groups (surgery only [S group] or surgery plus postoperative radiotherapy [S+RT group]) across substages of the 11th JES system according to independent prognostic factors for overall survival (OS) identified using the Cox proportional hazards regression. RESULTS: A total of 2960 patients were eligible. The 5-year OS AUC for the 8th AJCC system was significantly higher than that for the 11th JES system (0.701 vs. 0.675, P < 0.001). Before PSM, PORT significantly improved 5-year OS rates for patients in stage III and IVA by 9.1% (P < 0.001) and 21.1% (P < 0.001), respectively. After PSM, the 5-year OS rates in stage II, III, and IVA of the S+RT group were significantly higher than those in the S group (70.9%, 39.7%, and 35.1% vs. 57.8%, 27.2%, and 10.3%, respectively; P < 0.001). CONCLUSION: The 11th JES system was less capable of predicting prognosis than the 8th AJCC system and patients in stage III of the JES system were highly recommended to undergo PORT.

4.
Int J Radiat Oncol Biol Phys ; 111(3S): e363-e364, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34701275

RESUMO

PURPOSE/OBJECTIVE(S): The survival and larynx preservation were both important in hypopharyngeal cancer. For primary surgery, most patients need total laryngectomy. For primary radical concurrent chemoradiotherapy (CCRT), the non-responders to CCRT had worse outcome compared with surgery. Thus, our cancer center explored a new response-adapted strategy based on early response to radiotherapy. MATERIALS/METHODS: A total of 423 patients with resectable, III/IVA hypopharyngeal cancer were reviewed between May 2009 and October 2019. There were three main treatment strategies for locally advanced hypopharyngeal carcinoma in our institution, surgery followed by radiotherapy (S+RT group), response-adapted group and radical CCRT/RT (CRT group). The response-adapted strategy was determined by primary tumor regression evaluated at DT 50 Gy, only primary lesions reaching large PR were recommended for radical CCRT, otherwise planned surgery. We defined the survival with functional larynx as total laryngectomy or death from local disease. RESULTS: With a medial follow-up of 66.5 months, the 5-year overall survival (OS), locoregional recurrence-free survival (LRRFS), distance metastasis free survival (DMFS) and survival with functional larynx were 49.5%, 45.7%, 47.0% and 52.5% for whole cohort. For different groups, the 5-year OS, LRRFS, DMFS and survival with functional larynx were 54.4%, 52.5%, 52.3% and 47.4% for S+RT group (n = 144); 52.7%, 47.0%, 49.5% and 58.6% for response-adapted group (n = 212); 27.7%, 28.6%, 26.3% and 41.3% for CRT group (n = 67). The response-adapted strategy achieved similar survival and better survival with functional larynx compared with S+RT group, but the baseline between them were unbalanced. We use PSM to balance the two arms, the adjusted 5-year OS, LRRFS and DMFS were 54.8%, 48.7%, 53.5% for response-adapted group vs 52.2% 50.8%, 50.8% for S+RT group, without significant difference. But the adjusted 5-year larynx preservation rate and survival with functional larynx in response-adapted group were 75.1% and 53.8%, better compared with 56.1% and 50.1% in S+RT group (P < 0.01). The operative complications and acute toxicities (P > 0.05) for two groups had no difference. In subgroup analysis, 87.7% (186/212) required total laryngectomy when evaluated before treatment in the response-adapted group, but the 5-year survival with functional larynx were still 57.4%. In multivariable analysis, treatment strategies, ECOG, T and N stage were independently significant for OS, LRRFS, DMFS and survival with functional larynx. CONCLUSION: The response-adapted strategy achieved favorable survival with functional larynx and equally oncologist outcome compared with S+RT strategy. Besides, the response-adapted strategy did not result in additional operative complications and was well tolerated. Therefore, the response-adapted strategy might be an innovative, desirable for locally advanced hypopharyngeal cancer, especially for those with organ preservation intention.

5.
Int J Radiat Oncol Biol Phys ; 111(3S): e364, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34701278

RESUMO

PURPOSE/OBJECTIVE(S): Hypopharyngeal carcinoma (HPC) is one of the most devastating malignancies among head and neck cancers. Our aim is to analyze the clinical characteristics and prognosis of synchronous and metachronous second primary malignancies (SPMs) in patients with hypopharyngeal carcinoma. MATERIALS/METHODS: We retrospectively reviewed 673 patients diagnosed in our institution between May 2009 and October 2019. Endoscopic examination was performed in 97.1% (654/673) of patients before treatment. We defined a synchronous SPM as which developed within 6 months of the index HPC diagnosis, while a metachronous SPM as which developed more than 6 months after initial diagnosis of HPC. The Kaplan-Meier method, propensity score-matched (PSM) and Cox model were used to analyze the data. RESULTS: Within the median follow-up of 66.5 months, 71 (10.5%) patients developed gastric/esophageal tumor in situ (Tis) and 143 (21.2%), patients developed SPMs, consisting of 78 with synchronous SPMs and 75 with metachronous SPMs, respectively, with patient overlap. Among the 143 patients, the most common secondary site was esophagus (n = 85), followed by the head and neck squamous cell carcinoma (HNSCC, n = 36), lung (n = 20) and other cancers (n = 19). The 5-year overall survivals (OS) for non-SPMs (n = 459), SPMs (n = 143) and Tis group (n = 71) were 43.8% vs 37.1% vs 42.9%, respectively. Accordingly, the disease-specific survivals (DSS) were 46.3% vs 56.2% vs 47.8%, respectively. We compared synchronous SPMs and no-SPMs groups, the 5-year OS and DSS were 37.1% and 56.2% for synchronous SPMs group (n = 78), compared with 43.8% (P = 0.093) and 46.3% (P = 0.098) for no-SPMs group (n = 459). We use PSM to balance two arms with the ratio of 1:1, resulting in the number of 62 patients in each group. The adjusted 5-year OS and DSS were 23.6% and 32.7% for synchronous SPMs group (n = 62), compared with 49.8% (P < 0.001) and 52.4% (P < 0.001) for no-SPMs group (n = 62). For synchronous and metachronous SPMs, the 5-year OS and DSS were 27.3% and 40.8% for synchronous SPMs group (n = 78) vs 48.8% (P = 0.001) and 70.7% (P = 0.001) for metachronous SPMs group (n = 75). According to the site of the SPMs, the 5-year OS and DSS were 24.5% and 40.4% for esophagus (n = 85), 51.9% and 66.9% for HNSCC (n = 36), 52.0% and 75.1% for lung (n = 20), 60.7% and 83.9% for others (n = 19). The SPMs in esophagus had worse OS than the other three groups (P<0.05). In multivariable analysis, synchronous SPMs, site of the SPMs, alcohol consumption and N stage were independently significant for OS. CONCLUSION: Approximately 30% of patients with HPC develop multiple malignancies during the course of disease and the most frequently involved site is the upper aero digestive tract. We should be aware of the possible coexistence of SPMs in HPC. Co-existence of gastric/esophageal tumor in situ does not affect outcome. The OS and DSS for synchronous SPMs are significantly worse than that for metachronous SPMs and no-SPMs. The SPMs in esophagus is the most common site and present the worst outcome than others.

6.
Int J Radiat Oncol Biol Phys ; 111(3S): e482-e483, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34701557

RESUMO

PURPOSE/OBJECTIVE(S): The current treatment strategy for patients with metastatic nasopharyngeal carcinoma (NPC) is mainly based on palliative chemotherapy, with low complete remission rate and poor progression-free survival (PFS). Radiation therapy has proven efficacy in the treatment of patients with nonmetastatic NPC, with emerging indication in the setting of limited metastatic disease. We proposed a single-arm phase II study to determine if metastasis-directed SBRT plus systemic treatment could prolong PFS compared with historical findings at first-line therapy for patients with oligometastatic NPC. MATERIALS/METHODS: We enrolled metastatic NPC with 1-5 metastatic lesions, with all metastases amenable to SBRT. Prescribed doses ranged from 25-50 Gy administered in five fractions. All patients received systemic chemotherapy before or after SBRT. The primary endpoint was PFS at 1 year from the start of SBRT treatment. RESULTS: Between October 2016 and January 2020, 24 patients with a median age of 48 years (range, 29-65 years) were recruited. A total of 44 sites were treated with 11 of 24 patients receiving SBRT to more than one site. Spinal bone was most often irradiated. By Kaplan-Meier actuarial analysis, 1-year PFS was 62% and overall survival rate 87%. Most patients progressed in new distant sites with only one local SBRT failures out of 44 lesions. Patients with lung metastasis had a lower chance of distant progression than those with bone metastasis or liver metastasis. There were no SBRT relevant grade 3-5 toxicity, and only one patient suffered from grade 2 adverse event. CONCLUSION: Use of metastasis-directed SBRT with systemic chemotherapy for patients with oligometastatic NPC as first-line therapy was well tolerated, and resulted in high PFS, substantially greater than historical values for patients who only received systemic agents.

7.
Nan Fang Yi Ke Da Xue Xue Bao ; 41(9): 1296-1303, 2021 Aug 31.
Artigo em Chinês | MEDLINE | ID: mdl-34658342

RESUMO

OBJECTIVE: We propose a parallel neural network classification method to improve the performance of classification of 4 types of arrhythmias: normal beat, supraventricular ectopic beat, ventricular ectopic beat and fused beat. METHODS: Preprocessing was performed including denoising of ECG signal, segmentation of small-scale heartbeat and large-scale heartbeat and data enhancement. Based on deep learning theory, densely connected convolutional network was applied to improve the limitation of waveform feature extraction, and bidirectional long short-term memory network and efficient channel attention network were combined to enhance the function of time series features and important features of the waveform. The parallel network structure was adopted, and the waveform features of small- scale heartbeat and large-scale heartbeat were input to improve the accuracy of arrhythmia classification at the same time. Softmax was used to carry out the 4 classification tasks of arrhythmia by the parallel network model. RESULTS: The proposed method was verified using MIT-BIH Arrhythmia Database and 3 groups of experiments. The experiments for comparing the classification performance of multiple parallel network models and that of each classification model under different heartbeat input methods showed that the proposed classification model had an overall accuracy, average sensitivity and average specificity of 99.36%, 96.08% and 99.41%, respectively. Convergence performance analysis of the parallel network classification model showed that the training time of the classification model was 41 s. CONCLUSION: The parallel multi-network classification method can improve the average sensitivity, specificity and training time while maintaining a high overall accuracy, and may thus provide a new technical solution for clinical diagnosis of arrhythmia.


Assuntos
Aprendizado Profundo , Complexos Ventriculares Prematuros , Eletrocardiografia , Frequência Cardíaca , Humanos , Redes Neurais de Computação
8.
Nan Fang Yi Ke Da Xue Xue Bao ; 41(9): 1334-1341, 2021 Aug 31.
Artigo em Chinês | MEDLINE | ID: mdl-34658347

RESUMO

OBJECTIVE: To investigate the effects of RNA interference of long noncoding RNA FOXCUT on epithelial mesenchymal transformation and mitochondrial function in nasopharyngeal carcinoma (NPC) cells. METHODS: FOXCUT expression levels were detected by RT-PCR in tumor tissues and adjacent tissues from 50 patients with NPC and in NP69, CNE1, CNE2, SUNE2, HER2 and 5-8F cell lines. CNE1 cells were transfected with a short hairpin RNA (shRNA) targeting FOXCUT or a negative control RNA construct (shRNA-NC), and the changes in cell proliferation and morphology were assessed with CCK8 assay, clone formation assay and microscopic observation. An immunofluorescence assay was used to examine the vimentin-positive cells, and the levels of SOD, MDA and LDH in the cells were detected. The changes of mitochondrial membrane potential were detected with flow cytometry, and the expression levels of E-cad, N-cad, vimentin, Bax, Bcl-2, caspase-3 and c-Myc in the cells were detected with Western blotting. RESULTS: The expression level of FOXCUT was significantly increased in NPC tissues as compared with the adjacent tissues (P < 0.001). Compared with NP69 cells, CNE1, CNE2, SUNE2, HER2 and 5-8F cells all exhibited significantly increased expressions of FOXCUT (P < 0.001). In CNE1 cells, transfection with FOXCUT shRNA significantly inhibited cell proliferation and clone formation (P < 0.001), and caused obvious changes in cell morphology. FOXCUT knockdown significantly decreased the expressions of N-cad and vimentin, increased E- cad expression and the contents of MDA and LDH (P < 0.05), reduced vimentin- positive cells and the activity of SOD, and caused a shift of red fluorescent cells to green fluorescent cells and an increased percentage of green fluorescent cells. FOXCUT knockdown also resulted in significantly increased expressions of Bax/Bcl2 and cleaved Cas3/Cas3 and a lowered expression of c-Myc. CONCLUSIONS: Interference of FOXCUT can inhibit the proliferation and epithelial-mesenchymal transformation, enhance oxidative stress, induce mitochondrial function injury, and promote apoptosis in NPC cells, suggesting the potential of FOXCUT interference for targeted treatment of NPC.


Assuntos
Transição Epitelial-Mesenquimal , Mitocôndrias/patologia , Carcinoma Nasofaríngeo , Neoplasias Nasofaríngeas , RNA Longo não Codificante , Linhagem Celular Tumoral , Humanos , Carcinoma Nasofaríngeo/genética , Neoplasias Nasofaríngeas/genética , RNA Longo não Codificante/genética
9.
Public Health ; 198: 315-323, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34507138

RESUMO

OBJECTIVES: This study aimed to evaluate associations between mental distress and COVID-19-related changes in behavioral outcomes and potential modifiers (age, gender, educational attainment) of such associations. STUDY DESIGN: This was a cross-sectional study. METHODS: An online survey using anonymous network sampling was conducted in China from April to May 2020 using a 74-item questionnaire. A national sample of 10,545 adults in 31 provinces provided data on sociodemographic characteristics, COVID-19-related mental distress, and changes in behavioral outcomes. Structural equation models were used for data analyses. RESULTS: After adjusting for covariates, greater mental distress was associated with increased smoking (odds ratio [OR] = 1.42, 95% confidence interval [CI]: 1.20-1.68 and OR = 1.54, 95% CI: 1.31-1.82 per one standard deviation [SD] increase in mental distress) and alcohol consumption (OR = 1.67, 95% CI: 1.45-1.92 and OR = 1.47, 95% CI: 1.24-1.75 per one SD increase in mental distress) among current smokers and drinkers and with both increased and decreased physical activity (ORs ranged from 1.32 to 1.56). Underweight adults were more likely to lose body weight (≥1 kg; OR = 1.63, 95% CI: 1.30-2.04), whereas overweight adults were more likely to gain weight (OR = 1.61, 95% CI: 1.46-1.78) by the same amount. Association between mental distress and change in physical activity was stronger in adults aged ≥40 years (ORs ranged from 1.43 to 2.05) and those with high education (ORs ranged from 1.43 to 1.77). Mental distress was associated with increased smoking in males (OR = 1.60, 95% CI: 1.37-1.87) but not females (OR = 1.11, 95% CI: 0.82-1.51). CONCLUSIONS: Greater mental distress was associated with some positive and negative changes in behavioral outcomes during the pandemic. These findings inform the design of tailored public health interventions aimed to mitigate long-term negative consequences of mental distress on outcomes.


Assuntos
COVID-19 , Pandemias , Adulto , China/epidemiologia , Estudos Transversais , Humanos , Masculino , SARS-CoV-2
10.
Artigo em Chinês | MEDLINE | ID: mdl-34521172

RESUMO

Objective: To investigate the sonographic features of primary thyroid lymphoma (PTL) and to evaluate the clinical significance of ultrasound-guided core needle biopsy (US-CNB) in PTL. Methods: A total of 24 patients with suspected PTL in Sir Run Run Shaw Hospital from January 2013 to June 2018 were analyzed retrospectively. All cases were confirmed by pathology, of them 23 patients received US-CNB and 1 patient chose operation without US-CNB, including 5 males and 19 females, aged from 39 to 75 years old. The effectiveness and safety of 23 patients with US-CNB were evaluated, and the sonographic features of 20 patients with PTL diagnosed by pathology were analyzed. Descriptive statistical methods were used in the study. Results: In the 23 patients with suspected PTL underwent US-CNB, 18 patients were diagnosed as PTL, 4 patients were respectively diagnosed as subacute thyroiditis, anaplastic carcinoma, Hashimoto's thyroiditis, and fibro thyroiditis, and the another patient was hard to diagnose by US-CNB and then was diagnosed as PTL by surgical biopsy. The success rate of US-CNB for diagnosis of PTL was 18/19, and no severe complications occurred in the patients with US-CNB. The other case was diagnosed as PTL by surgical biopsy without US-CNB. Sonographic features of 20 cases with PTL (18 cases diagnosed by US-CNB and 2 cases by surgery or surgery biopsy) were as follows: (1) Most nodules had irregular shapes and unsmooth margins; (2) Hypoechoic or markedly hypoechoic nodules with honeycombed or cord structures were observed in most cases; (3) Calcification was rare; (4) Multiple lesions were common; (5) Abundant intralesional vascularization was commonly observed; (6) Most cases had intensification of posterior acoustic enhancement; (7) Thyroid gland enlargement or with irregular shape; and (8) PTL often accompanied with lymph nodes enlargement in lateral neck or central region. Conclusion: PTL has certain sonographic features, with assistance of US-CNB, more accurate diagnosis of PTL can be obtained.


Assuntos
Linfoma , Neoplasias da Glândula Tireoide , Nódulo da Glândula Tireoide , Adulto , Idoso , Biópsia com Agulha de Grande Calibre , Feminino , Humanos , Biópsia Guiada por Imagem , Linfoma/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Glândula Tireoide , Neoplasias da Glândula Tireoide/diagnóstico por imagem , Ultrassonografia de Intervenção
12.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 56(8): 816-820, 2021 Aug 09.
Artigo em Chinês | MEDLINE | ID: mdl-34404150

RESUMO

Effective removal of dental plaque is a key measure to prevent and control common oral diseases and to maintain the long-term effect of dental prostheses. The methods of oral plaque control are mainly depending on mechanical measures, including regular toothbrushing, interdental cleaning and oral irrigation. Toothbrushing is the most common and effective method for mechanical removal of dental plaque.How ever the effect of toothbrushing alone to remove dental plaque is limited. It is necessary to control the interdental plaque using tools such as dental floss and interdental brushes. Oral irrigation is an assistant method to improve oral hygiene. Toothbrushing combined with the use of oral irrigator can significantly improve the removal rate of dental plaque. The present article reviews the research progress of the mechanical plaque control methods and their application results.

13.
Zhonghua Xin Xue Guan Bing Za Zhi ; 49(8): 790-795, 2021 Aug 24.
Artigo em Chinês | MEDLINE | ID: mdl-34404188

RESUMO

Objective: To analyze the impact of cancer on the recurrence rate of atrial fibrillation (AF) after AF radiofrequency ablation and further evaluate the feasibility of radiofrequency ablation therapy in cancer patients with AF. Methods: This study was a single-center, retrospective study. Cancer patients with AF undergoing radiofrequency ablation for the first time in the First Affiliated Hospital of Dalian Medical University from May 30, 2008 to September 30, 2018 were included (cancer group). AF patients without cancer undergoing radiofrequency ablation for the first time during the same period served as non-cancer group. Clinical data including age, gender, past history, cancer and AF-related parameters, etc. were analyzed. Patients were followed up after radiofrequency ablation. The primary endpoints were AF recurrence or all-cause death. Kaplan-Meier survival analysis was used to analyze the effect of cancers on the recurrence after AF ablation. The multivariate cox regression analysis was further applied to correct for other confounding factors to analyze whether the impact of cancers on the recurrence of atrial fibrillation was statistically significant. Results: A total of 90 patients were enrolled, there were 30 patients in the cancer group (mean age (64.8±6.6) years, 16 (53.3%) males) and 60 patients in the non-cancer group (mean age (63.6±6.2) years, 32 (53.3%) males). Clinical data, such as age, gender, and cancer treatment, were similar between the two groups. During an average follow-up period of (328.7±110.2) days, there were 6 AF recurrences (recurrence rate 20.0%) in the cancer group, and 17 AF recurrences (recurrence rate 28.3%) in the control group. AF recurrence rate was similar between the two groups (P>0.05). During the follow-up period, there was no all-cause death in the two groups. Kaplan-Meier survival analysis showed that cancer was not related to AF recurrence after radiofrequency ablation (P = 0.383). After adjusting for other confounding factors, the multivariate Cox regression analysis showed that cancer was not an independent predictor of AF recurrence after radiofrequency ablation (HR=0.508, 95%CI: 0.192-1.342, P = 0.172). Conclusions: The combination of cancer has no impact on the recurrence of AF after radiofrequency ablation. For cancer patients with AF, radiofrequency ablation therapy can be considered as a feasible heart rhythm control treatment strategy.

14.
Nan Fang Yi Ke Da Xue Xue Bao ; 41(7): 1002-1011, 2021 Jul 20.
Artigo em Chinês | MEDLINE | ID: mdl-34308849

RESUMO

OBJECTIVE: To investigate the protective effect of Bao Gan Ning (BGN), a traditional Chinese medicinal formula, against CCl4-induced liver fibrosis in mice and explore the mechanism. METHODS: C57BL/6 mice were randomly divided into control group, liver fibrosis model group, positive control group and the low-, middle-, and high-dose BGN groups (n=10). In all but the control group, the mice were subjected to daily intraperitoneal injection of 25% CCl4 (in olive oil) to induce liver fibrosis and intragastric gavage of corresponding drugs. After 8 weeks, serum levels of ALT and AST were detected. Pathological examination of the liver was performed using HE and Sirius Red staining and α-SMA immunohistochemistry. The expression level of indoleamine 2, 3-dioxygenase 1 (IDO1) and phenotypic changes of hepatic DCs in the liver were measured. Another 18 mice were randomly divided into AAV9-NC, AAV9-IDO1 and high-dose BGN + AAV-IDO1 groups (n=6) for corresponding treatment, and 4 weeks later the deposit of hepatic IDO1 and phenotypic changes of the hepatic DCs were analyzed. RESULTS: Compared with those in the model group, serum AST and ALT levels decreased significantly in BGN group (P < 0.01). Obvious liver fibrosis was observed in the model group, while the mice treated with BGN showed obviously reduced cell necrosis and collagen proliferation in the liver with significantly lowered the expression levels of hepatic α-SMA and IDO1 (P < 0.05). The percentages of CD11C + DCs, CD11C +CD80 + DCs, CD11C +CD86 + DCs, CD11C +CD40 + DCs, CD11C +MHCII + DCs, CD3 + T cells, and CD3 +CD4 + T cells all increased significantly in BGN group as compared with the model group (P < 0.05). In mice with adenovirus-mediated IDO1 overexpression in the liver, BGN treatment significantly lowered the expression level of IDO1 (P=0.000) and increased the percentages of hepatic CD11C +CD40 + DCs, CD11C +MHCII + DCs and CD3 +CD4 + T cells (P < 0.05). CONCLUSION: BGN can effectively inhibit liver fibrosis in mice possibly by lowering the expression level of IDO1 in the liver, thus improving the function of hepatic DCs and subsequently promoting proliferation of T cells.


Assuntos
Cirrose Hepática , Fígado , Animais , Células Dendríticas , Fígado/patologia , Cirrose Hepática/tratamento farmacológico , Camundongos , Camundongos Endogâmicos C57BL , Linfócitos T
16.
Clin Transl Oncol ; 2021 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-34318428

RESUMO

PURPOSE: Increasing evidences suggest dysfunctions of microRNAs (miRNAs) are playing important part in tumors. Therefore, the role of miR-802 in osteosarcoma (OS) was exploited. The object was to evaluate the effect of miR-802 and verify its influence on p27 Kip1 (p27) in OS. METHODS: RT-qPCR experiment was used to detect miR-802 and p27 expression in OS tissues and cells. We explored the function of miR-802 through Transwell assays. The phosphoinositide 3-kinase (PI3K)/AKT serine/threonine kinase pathway and epithelial-mesenchymal transition (EMT) was detected by Western blot assays. Luciferase assay was used to testify the target of miR-802. RESULTS: MiR-802 expression was elevated in OS, which was related to poor clinical outcome in OS patients. MiR-802 overexpression promoted OS migration, invasion and EMT. Further, p27 is a direct target of miR-802. P27 elevation counteracted the promotion effect of OS on EMT, migration and invasion induced by miR-802. In addition, miR-802 overexpression inactivated PI3K/AKT pathway via targeting p27 in OS. CONCLUSION: MiR-802 promoted the progress of EMT, migration and invasion in OS via targeting p27. This newly identified miR-802/p27/PI3K/AKT axis may represent potential targets for OS.

17.
Rev Sci Instrum ; 92(5): 053102, 2021 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-34243250

RESUMO

We report the development of a high-resolution spectrometer for extended x-ray absorption fine structure (EXAFS) studies of materials under extreme conditions. A curved crystal and detector in the spectrometer are replaceable such that a single body is employed to perform EXAFS measurements at different x-ray energy intervals of interest. Two configurations have been implemented using toroidal crystals with Ge 311 reflection set to provide EXAFS at the Cu K-edge (energy range 8.9-9.8 keV) and Ge 400 reflection set to provide EXAFS at the Ta L3-edge (9.8-10.7 keV). Key performance characteristics of the spectrometer were found to be consistent with design parameters. The data generated at the National Ignition Facility have shown an ≃3 eV spectral resolution for the Cu K-edge configuration and ≃6 eV for the Ta L3-edge configuration.

18.
Rev Sci Instrum ; 92(4): 043503, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-34243373

RESUMO

Edge turbulence is important for plasma confinement, so the gas puffing imaging (GPI) diagnostic was proposed on the J-TEXT tokamak for the two-dimensional measurement of turbulence in the edge region. GPI is a diagnostic of plasma turbulence that uses a puff of neutral gas at the plasma edge to increase the local visible light emission for improved space-time resolution of plasma fluctuations. Considering the conditions of J-TEXT, the observation area is 21° away from the position of the optical system in the toroidal direction, and the observation area is 10 cm × 10 cm inside and outside the last closed flux surface. To have a lower divergence of the gas flow, the gas puff nozzle is specially designed. An interface has been developed for operation. To photograph the line radiation generated by the neutral gas cloud along the magnetic field lines, the optical system is designed. It is composed of a quartz glass, mirrors, commercial lenses, filters, and high-speed cameras. The high-speed camera can capture the line radiation with a speed up to 180 000 frames/s with 256 pixels × 256 pixels and an exposure time of 5 µs. In a recent experiment, the new GPI diagnostic has obtained some preliminary pictures.

19.
Zhonghua Zhong Liu Za Zhi ; 43(6): 678-684, 2021 Jun 23.
Artigo em Chinês | MEDLINE | ID: mdl-34289560

RESUMO

Objective: To summarize survival outcomes and prognostic factors in esophageal cancer (EC) patients treated with intensity-modulated radiotherapy (IMRT). Methods: A retrospective analysis was performed on the clinical and follow-up data of 1 637 patients with EC who were admitted to our hospital from January 2005 to December 2017 and met the inclusion criteria.The 5-year overall survival (OS), progression-free survival (PFS) and pattern of recurrence were analyzed. The Kaplan-Meier method was used to calculate survival rates, Log-rank test for univariate analysis and Cox method for multivariate analysis were used to detect survival difference. Results: 1-year, 3-year and 5-year OS and PFS of the entire group were 65.9% and 45.8%, 34.2% and 25.0%, 27.0% and 18.5%, respectively. Median OS and PFS were 19.4 months (95% CI=18.0-20.7 months) and 10.4 months (95% CI=9.3-11.3 months), respectively. Univariate analysis showed that the sex, KPS, tumor location, T stage, N stage, M stage, TNM stage, radiation dose and treatment modality were prognostic factors for 5-year OS and PFS of EC patients (P<0.05). Multivariate analysis indicated that the sex, KPS, TNM stage, radiation dose and treatment modality were independent prognostic factors for 5-year OS and PFS (P<0.05). Conclusions: EC patients treated with IMRT can obtain a promising survival. The sex, KPS, TNM stage, radiation dose and treatment modality are independent prognostic factors for prognosis.


Assuntos
Neoplasias Esofágicas , Radioterapia de Intensidade Modulada , Intervalo Livre de Doença , Neoplasias Esofágicas/patologia , Neoplasias Esofágicas/radioterapia , Humanos , Recidiva Local de Neoplasia , Estadiamento de Neoplasias , Prognóstico , Estudos Retrospectivos
20.
Beijing Da Xue Xue Bao Yi Xue Ban ; 53(3): 479-484, 2021 Jun 18.
Artigo em Chinês | MEDLINE | ID: mdl-34145848

RESUMO

OBJECTIVE: To test the reliability and validity of the Chinese version of parenting sense of competence scale (PSOC) in Chinese mothers of preschool children, and to explore the perception of preschool children's mothers on their own parenting skills and their comfort of being a parent in Yanqing District of Beijing. METHODS: A cross-sectional survey was conducted using a convenience sample in 1 384 preschool children's mothers in Yanqing District of Beijing. SPSS 21.0 and Mplus 7.4 software were used for statistical analysis to test the structural validity, criterion related validity, internal consistency and split half reliability of the scale, and to analyze the score of the scale and its influencing factors. RESULTS: The PSOC had good reliability and validity. Exploratory factor analysis showed that each item of the PSOC had more than 0.4 factor loading in efficacy factor or satisfaction factor, and there was no double load phenomenon. Confirmatory factor analysis showed that the factor loadings ranged from 0.212 to 0.843 in efficacy factor and satisfaction factor, respectively. The goodness of fit test showed that all the fitting indexes were within the acceptable range, and the correlation between the effectiveness subscale and the satisfaction subscale was high. The Cronbach's α coefficient of the whole scale, the efficacy subscale and the satisfaction subscale were 0.872, 0.802, and 0.874, respectively. The Spearman-Brown coefficient of PSOC was 0.851. The average score of the whole scale, the efficacy subscale, and the satisfaction subscale were 72.33±11.31, 35.54±5.91, and 36.79±7.11, respectively, and the score of parenting competence in Chinese mothers of preschool children was influenced by the mother's educational level and the annual income of her family. CONCLUSION: The PSOC has satisfactory reliability and validity in Chinese mothers of preschool children. It can be used as an evaluation instrument for measuring the parenting competency, self perceived efficacy and satisfaction in the mainland Chinese mothers of preschool children. The competency of preschool children's mothers in Yanqing District of Beijing is very good, which may be related to the higher education level of the mothers and the higher annual income of their families in this study.


Assuntos
Mães , Poder Familiar , Pequim , Pré-Escolar , China , Estudos Transversais , Feminino , Humanos , Psicometria , Reprodutibilidade dos Testes , Inquéritos e Questionários
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