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1.
J Am Dent Assoc ; 152(3): 189-201.e1, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33632408

RESUMO

BACKGROUND: The impact of hyperglycemia on dental implant therapy remains unclear. In this systematic review and meta-analysis, the authors compared the rates of implant failure and peri-implant bleeding on probing (BOP), probing depth (PD), and peri-implant bone loss (PIBL) among patients with type 2 diabetes mellitus and nondiabetic patients. The authors performed subgroup analyses based on glycemic level to evaluate whether patients with higher glycemic levels were more prone to peri-implant inflammation. TYPE OF STUDIES REVIEWED: The authors searched 4 databases for original clinical studies. Studies in which the researchers provided information on the rate of implant failure or peri-implant parameters were included. RESULTS: Nine clinical studies were identified on the basis of the inclusion criteria. No significant differences were found in rates of implant failure (P = .46) and PD (P = .1) between diabetic and nondiabetic patients. Significant differences in BOP (P < .00001) and PIBL (P = .02), favoring nondiabetic patients, were observed. Results of subgroup analyses indicated that the increase in glycemic level did not significantly influence BOP, PD, and PIBL values among diabetic patients. CONCLUSIONS AND PRACTICAL IMPLICATIONS: Patients with type 2 diabetes mellitus seem to be able to achieve a rate of implant survival similar to that of healthy patients. Regarding peri-implant parameters, BOP and PIBL were higher in patients with type 2 diabetes mellitus, indicating that hyperglycemia is an important risk factor for peri-implant inflammation. No association was found between peri-implant parameters and glycemic level among patients with type 2 diabetes mellitus, providing oral hygiene was strictly maintained.

2.
Ann Hematol ; 2021 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-33389024

RESUMO

Tumour-infiltrating lymphocytes (TILs) account for a large proportion of tumour microenvironment (TME) in angioimmunoblastic T cell lymphoma (AITL), and at present the significance of TIL in TME of AITL remains unclear. Overall, 50 de novo AITL patients undergoing lymph node flow cytometry from 2014 to 2019 were retrospectively analysed to assess the relationship between TILs and AITL prognosis. We found that high TIL-Bs (≥ 42.4%, p = 0.004) and high CD4:CD8 (≥ 0.85, p = 0.024) were independent favourable prognostic factors for de novo AITL in univariate or multivariate analyses. New TIL-related risk stratification was established based on TIL-Bs and CD4:CD8 factors. Patients in the low-risk group (TIL-Bs ≥ 42.4% and CD4:CD8 ≥ 0.85) had significantly better overall survival than the high-risk (TIL-Bs < 42.4% and CD4:CD8 < 0.85) (p < 0.001) or intermediate-risk group (TIL-Bs ≥ 42.4% and CD4:CD8 < 0.85 or TIL-Bs < 42.4% and CD4:CD8 ≥ 0.85) (p = 0.011). To our knowledge, our cohort is the largest one focusing on the TILs in de novo cases of AITL by analysing lymph node samples using flow cytometry, which is the first time to comprehensively consider humoral immunity and cellular immunity influence on AITL. Our new risk stratification was valuable and useful in evaluating prognosis of AITL and guiding immunotherapy strategies.

3.
Biomed Res Int ; 2020: 7926754, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33354570

RESUMO

The aim of this study is to reveal the certain human papillomavirus (HPV) genotype distribution between cervical cancer and esophageal cancer in the both high-incidence geographic regions. For this study, we collected and detected the infection of HPV in 120 paraffin-embedded esophageal tissues and 152 paraffin-embedded cervical tissues, respectively. The esophageal tissues include 40 normal epithelium (ENOR), 26 dysplasia (DYS), and 54 invasive squamous cell carcinoma (ESCC). The cervical tissues consisted of 40 normal epithelium (CNOR), 53 intraepithelial neoplasia (CIN), and 59 invasive squamous cell carcinoma (CSCC). Both esophageal and cervical tissues collected in this study came from the same area, in which both the ESCC and CSCC were in high incidence, Xinjiang province, China. HPV GenoArray test kits were served to analyze the HPV infection. The result shows that among the 59 CSCC tissues, the total infection rate of HPV was 98.3% (58/59). The positive rate of HPV-16 infection was 63.8% (37/58). It indicated that HPV-16 is the most common infection among all of the high-risk HPV. The multiple infection rate was 19.0% (11/58). Among the 54 ESCC, a total of 7 genotypes were detected. The total infection rate of HPV was 61.1% (33/54). The positive rate of HPV-16 infection was 63.6% (21/33). The multiple infection rate was 6.1% (2/33). Our result shows that high-risk-type HPV-16 was associated with both cervical cancer and esophageal cancer, which play a role in the high-incidence area in Xinjiang. We hope that our results could point out the direction for the treatment strategy of HPV-associated cancer, cervical cancer, and esophageal cancer and for the application of HPV vaccines in the future.

4.
Sensors (Basel) ; 20(21)2020 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-33167462

RESUMO

Five kinds of new homo-polymer and copolymers of methacrylate containing a fluorine ester group were synthesized and used for the binder of pressure-sensitive paint (PSP)to ensure the good compatibility between luminophore (Pt(II) meso-tetra (pentafluorophenyl) porphine (PtTFPP)) and polymer binder. In the work, we were concerned with how the structure of thesepolymers containing fluorine, especially the various ester group structure, affects the response frequency of PSP using oscillating sound wave technique. The results showed that the pressure sensitivities (Sp) of these PSP samples containing different polymers, exhibit some difference. The length of ester chain on the methacrylatepolymer affects the response frequency of PSP sensor layer composed of the polymer. The longer the chain length of the ester group, the higher the response frequency of the PSP sensor layer quenching by oxygen.

5.
BMC Plant Biol ; 20(1): 527, 2020 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-33208102

RESUMO

BACKGROUND: The ratio of CO2 mesophyll conductance (gm) to Ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) content has been suggested to positively affect photosynthetic nitrogen use efficiency (PNUE). The anatomical basis of gm has been quantified, but information on the relationship between cell-level anatomies and PNUE is less advanced. Here, hydroponic experiments were conducted in rice plants supplied with ammonium (NH4+) and nitrate (NO3-) under three N levels (low, 0.71 mM; intermediate, 2.86 mM; high, 7.14 mM) to investigate the gas exchange parameters, leaf anatomical structure and PNUE. RESULTS: The results showed a lower PNUE in plants supplied with high nitrogen and NH4+, which was positively correlated with the gm/Rubisco ratio. A one-dimensional within-leaf model revealed that the resistance to CO2 diffusion in the liquid phase (rliq) dominated the overall mesophyll resistance (rm), in which CO2 transfer resistance in the cell wall, cytoplasm and stroma were significantly affected by nitrogen supply. The chloroplast surface area exposed to intercellular space (Sc) per Rubisco rather than the gm/Sc ratio was positively correlated with PNUE and was thus considered a key component influencing PNUE. CONCLUSION: In conclusion, our study emphasized that Sc was the most important anatomical trait in coordinating gm and PNUE with contrasting N supply.

6.
Biochem Biophys Res Commun ; 530(3): 574-580, 2020 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-32753318

RESUMO

Inflammation and the proliferation of vascular smooth muscle cells (VSMCs) are seen to play critical roles in the development of vascular complications induced by diabetes and hyperglycemia. Dihydroartemisinin (DHA) has been identified as a semi-synthetic derivative of artemisinin that exhibits broad protective effects. However, the effect of DHA on high glucose (HG)-induced inflammation and proliferation of VSMCs remains unknown. Therefore, this study aims to show that DHA significantly inhibited the proliferation of VSMCs and that expression of the inflammatory cytokines IL-1ß and TNF-α was induced by HG in a dose-dependent manner. Additionally, we were able to determine that KLF15 played a critical role in HG-induced VSMC proliferation and inflammation, confirming its protective effects observed after DHA treatment in the HG-induced inflammatory response of VSMCs. DHA was observed to directly depress the HG-induced expression of miR-376b-3p, which targeted the 3'-UTR of KLF15 and inhibited its expression. These results suggested that DHA plays a protective role in HG-induced VSMC proliferation and associated inflammation by inhibiting the miR-376b-3p/KLF15 axis. Our findings provide new evidence of the mechanisms of DHA and its critical role in treating the pathogenesis of diabetic vascular complications.

7.
Medicine (Baltimore) ; 99(27): e21119, 2020 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-32629746

RESUMO

INTRODUCTION: Myeloid sarcoma (MS) is a rare tumor mass. It may occur at any extramedullary anatomic sites but is uncommon in the sinonasal location.MS commonly presents concurrently with acute myeloid leukemia (AML), but it may predate AML over several months or years, named isolated MS. PATIENT CONCERNS: We report a case of a 15-month-old child who presented with mouth breathing, bilateral rhinorrhea, palpebral edema and proptosis. The routine blood tests were normal for the first few months. Computed tomography scan revealed neoplasm in nasal cavity. DIAGNOSIS: The patient was definitely diagnosed with isolated MS in the nasal cavity through immunohistochemistry combined with clinical features and radiological investigations, and MS further progressed to AML which was confirmed by hematologist. INTERVENTIONS: Endoscopic sinus surgery was performed to acquire specimens. After diagnosis, the patient was promptly treated with systemic chemotherapy. OUTCOMES: All symptoms gradually subsided and the mass of nasal cavity was invisible. No relapse occurred during follow-up. CONCLUSION: Sinonasal MS may be misdiagnosed and should be considered when symptoms persist and worsen. Prompt clinic examinations are essential for cases with suspected MS. Diagnosis of MS is dependent on the immunohistological investigations combined with clinical features, radiological investigations. Early diagnosis and systemic chemotherapy are vital for patients to achieve best prognosis.


Assuntos
Leucemia Mieloide Aguda/tratamento farmacológico , Cavidade Nasal/diagnóstico por imagem , Sarcoma Mieloide/complicações , Antineoplásicos/administração & dosagem , Antineoplásicos/uso terapêutico , Erros de Diagnóstico/prevenção & controle , Diagnóstico Precoce , Edema/etiologia , Exoftalmia/etiologia , Doenças Palpebrais/patologia , Humanos , Imuno-Histoquímica/métodos , Lactente , Leucemia Mieloide Aguda/diagnóstico , Leucemia Mieloide Aguda/cirurgia , Masculino , Cavidade Nasal/patologia , Cavidade Nasal/cirurgia , Sarcoma Mieloide/diagnóstico por imagem , Sarcoma Mieloide/metabolismo , Tomografia Computadorizada por Raios X/métodos , Resultado do Tratamento
8.
Cancer Biol Ther ; 21(9): 785-789, 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32552305

RESUMO

Intrahepatic cholangiocarcinoma (iCCA) is a highly aggressive malignancy with a poor prognosis. There is no standard treatment beyond first-line chemotherapy and no molecular-targeted drug approved for advanced iCCA. We herein present a case of a 46-y-old Asian iCCA patient with multiple metastases in lung, bone, and liver. The patient progressed rapidly after first- and second-line chemotherapy. According to next-generation sequencing result of somatic Von Hippel-Lindau (VHL) gene mutation, the patient was administered third-line sunitinib and obtained a relatively longer survival of 9 months after taking sunitinib. Additionally, we briefly summarized the current targeted treatment of iCCA. To our knowledge, this is the first report of VHL mutation and sunitinib usage in metastatic iCCA patient. As a highly heterogeneous and aggressive malignancy, we strongly recommend making clinical decisions based on precision medicine concept in advanced iCCA.

9.
Environ Sci Pollut Res Int ; 27(27): 34421-34433, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32557021

RESUMO

Italian ryegrass (Lolium multifolorum Lam.) has a potential phytoextraction capacity for cadmium (Cd), which is considered as the most toxic heavy metal (HM) pollutant in the farmland. The promotion effect of urea application on Italian ryegrass growth has been clarified, while it is not clear whether and how urea application affects Cd accumulation in Italian ryegrass under Cd stress. A 2-year pot experiment was conducted to investigate the effect of urea application on Cd accumulation and related mechanisms by uptake inhibition and kinetics experiments. The results showed that both shoot biomass and Cd concentration under Cd stress were increased by up to 213.37% and 84.74% in 2016 and 38.15% and 47.11% in 2017 after urea application, respectively. The shoot Cd accumulation reached maximum value (910.23 and 630.09 µg pot-1 in 2016 and 2017, respectively) at the level of 300 kg ha-1 urea. Superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities were significantly increased by urea application. Compared with control group, urea application significantly improved inhibition ratio of 2, 4-dinitrophenol (DNP) rather than LaCl3 and Ca2+. Cadmium uptake kinetics experiment showed that urea application significantly decreased the Km value and improved the α value (P < 0.01), but no significant effect on the Vmax value (P > 0.05). In conclusion, we proposed that the higher affinity to Cd2+ of the membrane transporter after urea application promoted the active uptake of Cd, which contributed to the effective Cd accumulation capacity in Italian ryegrass.


Assuntos
Lolium , Poluentes do Solo/análise , Biodegradação Ambiental , Cádmio/análise , Itália , Raízes de Plantas/química , Ureia
10.
Am J Surg Pathol ; 44(8): 1061-1072, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32317607

RESUMO

Systemic Epstein-Barr virus-positive T-cell and natural killer (NK)-cell lymphoproliferative diseases of childhood are a group of lethal diseases mostly affecting children and young adults. The Ohshima Grading System and the 2017 World Health Organization (WHO) classification have been used for classifying this spectrum, but these systems have not been validated externally and compared. Therefore, we examined 36 cases of systemic Epstein-Barr virus-positive T-cell and NK-cell lymphoproliferative diseases of childhood with long-term follow-up, from Southwest China, to systematically summarize the clinicopathologic features and to validate and compare the Ohshima Grading System and the 2017 WHO classification in discrimination ability, predictive accuracy, concordance indices, and explained variation. Clinically, our cohort showed severe manifestations and poor prognoses. Morphologically, the hematopoietic and lymphoid specimens showed proliferation of small-sized to medium-sized bland-looking lymphocytes that might mask disease severity, whereas other extranodal lesions showed a disorganized to obliterated architecture infiltrated by medium-sized to large-sized, subtle to obvious atypical cells, which may mimic extranodal NK/T-cell lymphoma. Immunophenotypically, our cases mainly originate from CD8 αß T cells. Therefore, clinical and pathologic features should be equally considered to avoid missed diagnosis or misdiagnosis. In addition, the 2017 WHO classification shows a flexible grasp of pathologic features, thus classifying some cases (polymorphic and monoclonal cases with fulminant course) more reasonably; thereby, it showed statistically improved results compared with the Ohshima Grading System. However, underestimating the risk of some polyclonal cases and imprecisely discriminating monoclonal cases at diagnosis are common dilemmas in both systems. Therefore, the construction of a comprehensive grading algorithm for improved prognostic value and precise diagnosis requires additional studies.


Assuntos
Infecções por Vírus Epstein-Barr/classificação , Herpesvirus Humano 4/genética , Células Matadoras Naturais/imunologia , Transtornos Linfoproliferativos/classificação , Linfócitos T/imunologia , Adolescente , Adulto , Idade de Início , Proliferação de Células , Criança , Pré-Escolar , China , Bases de Dados Factuais , Infecções por Vírus Epstein-Barr/imunologia , Infecções por Vírus Epstein-Barr/patologia , Infecções por Vírus Epstein-Barr/virologia , Feminino , Genes Codificadores da Cadeia alfa de Receptores de Linfócitos T , Genes Codificadores da Cadeia beta de Receptores de Linfócitos T , Humanos , Lactente , Células Matadoras Naturais/patologia , Transtornos Linfoproliferativos/imunologia , Transtornos Linfoproliferativos/patologia , Transtornos Linfoproliferativos/virologia , Masculino , Pessoa de Meia-Idade , Prognóstico , RNA Viral/genética , Estudos Retrospectivos , Medição de Risco , Fatores de Risco , Linfócitos T/patologia , Fatores de Tempo , Adulto Jovem
11.
Cytometry B Clin Cytom ; 98(1): 28-35, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31313887

RESUMO

BACKGROUND: Extranodal natural killer (NK)/T-cell lymphoma, nasal type (ENKTL-N) is an aggressive lymphoma typically diagnosed by examining small biopsy specimens. Flow cytometry is very valuable for the diagnosis and classification of several kinds of hematolymphoid neoplasms but has not been widely used for diagnosing ENKTL-N. METHODS: We systematically investigated the flow cytometry characteristics of 26 solid tissue biopsy specimens of ENKTL-N at initial diagnosis and compared the results with those from reactive NK-cells in the nasal/nasopharyngeal region and peripheral blood. RESULTS: Our study revealed seven flow cytometry (FCM)-based characteristics for distinguishing between the neoplastic cells and reactive NK-cells, including (1) the proportion of NK-cells among total lymphocytes >10%; (2) forward scatter >105 ; (3) mean fluorescence intensity of CD56 > 5,000; (4) aberrant antigen expression or loss; (5) skewed killer cell immunoglobulin-like receptor repertoire; (6) homogenously positive for CD38; and (7) positive for CD30 or CD336. FCM-based immunophenotyping is a potentially feasible and convenient approach for discriminating cellular lineages, evaluating the activation status of NK-cells, and selecting potential therapy targets of ENKTL-N. CONCLUSIONS: Flow cytometry is very valuable for facilitating routine diagnosis, confirming clonality, predicting the cellular lineage, and guiding individual treatment for ENKTL-N. © 2019 International Clinical Cytometry Society.

12.
New Phytol ; 226(1): 232-243, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-31778576

RESUMO

In the processes controlling ecosystem fertility, fungi are increasingly acknowledged as key drivers. However, our understanding of the rules behind fungal community assembly regarding the effect of soil fertility level remains limited. Using soil samples from typical tea plantations spanning c. 2167 km north-east to south-west across China, we investigated the assemblage complexity and assembly processes of 140 fungal communities along a soil fertility gradient. The community dissimilarities of total fungi and fungal functional guilds increased with increasing soil fertility index dissimilarity. The symbiotrophs were more sensitive to variations in soil fertility compared with pathotrophs and saprotrophs. Fungal networks were larger and showed higher connectivity as well as greater potential for inter-module connection in more fertile soils. Environmental factors had a slightly greater influence on fungal community composition than spatial factors. Species abundance fitted the Zipf-Mandelbrot distribution (niche-based mechanisms), which provided evidence for deterministic-based processes. Overall, the soil fungal communities in tea plantations responded in a deterministic manner to soil fertility, with high fertility correlated with complex fungal community assemblages. This study provides new insights that might contribute to predictions of fungal community complexity.

13.
Plant Sci ; 290: 110267, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31779911

RESUMO

Both nitrogen (N) and potassium (K) have been widely studied in maintaining efficient photosynthesis and plant growth. However, the mechanisms underlying the photosynthetic response to their interaction remain unclear. This study assessed the effects of N and K supply on photosynthetic limitations and the corresponding changes in anatomical structures in leaves of rice (Oryza sativa L.) plants, grown hydroponically under different levels of N and K in a greenhouse. Results revealed that a suitable leaf N/K ratio (2.99-3.10) maintain a high rate of photosynthesis (A). The A under N and/or K deficiency was primarily limited by mesophyll conductance (gm) and RuBP carboxylation in biochemical processes. The decline of gm in N- or K-starved leaves was mostly resulted from low surface area of chloroplasts exposed to intercellular airspaces (Sc) and high mesophyll cell wall thickness. Synergistic effects of N and K on gm were reflected in leaf anatomical structure, especially their coordinated roles in enhancing Sc. The enhanced photosynthesis in plants with coordinated supply of N and K was caused by the balance of RuBP carboxylation and regeneration. These results highlight the synergistic effect of N and K on leaf photosynthesis, which are mainly reflected in facilitating anatomical-determined gm and carboxylation capacity.


Assuntos
Nitrogênio/metabolismo , Oryza/anatomia & histologia , Oryza/metabolismo , Fotossíntese , Potássio/metabolismo , Transporte Biológico , Dióxido de Carbono/metabolismo , Folhas de Planta/anatomia & histologia , Folhas de Planta/metabolismo
14.
Sci Rep ; 9(1): 12250, 2019 08 22.
Artigo em Inglês | MEDLINE | ID: mdl-31439882

RESUMO

The genus Allium is one of the largest monocotyledonous genera, containing over 850 species, and most of these species are found in temperate climates of the Northern Hemisphere. Furthermore, as a large number of new Allium species continue to be identified, phylogenetic classification based on morphological characteristics and a few genetic markers will gradually exhibit extremely low discriminatory power. In this study, we present the use of complete chloroplast genome sequences in genome-scale phylogenetic studies of Allium. We sequenced and assembled four Allium chloroplast genomes and retrieved five published chloroplast genomes from GenBank. All nine chloroplast genomes were used for genomic comparison and phylogenetic inference. The chloroplast genomes, ranging from 152,387 bp to 154,482 bp in length, exhibited conservation of genomic structure, and gene organization and order. Subsequently, we observed the expansion of IRs from the basal monocot Acorus americanus to Allium, identified 814 simple sequence repeats, 131 tandem repeats, 154 dispersed repeats and 109 palindromic repeats, and found six highly variable regions. The phylogenetic relationships of the Allium species inferred from the chloroplast genomes obtained high support, indicating that chloroplast genome data will be useful for further resolution of the phylogeny of the genus Allium.


Assuntos
Allium/genética , Genoma de Cloroplastos , Filogenia , Allium/classificação , Cloroplastos/genética , DNA de Cloroplastos/genética , Evolução Molecular , Genoma de Planta , Repetições de Microssatélites
15.
Cancer Biol Ther ; 20(10): 1319-1327, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31311407

RESUMO

Limited studies are available on the molecular pathogenesis of Epstein-Barr virus (EBV)-associated T or natural killer (NK) cell lymphoproliferative disorders (EBV+T/NK-LPD). In this retrospective study, we aim to elucidate the mutation profile of EBV+T/NK-LPD using capture-based targeted sequencing with a panel consisting of 64 lymphoma-related genes to identify driver genes associated with the development of EBV+T/NK-LPD. Targeted sequencing of 169 EBV+T/NK-LPD cases was performed using a panel of 64 lymphoma-related genes. Of the 169 EBV+T/NK-LPD cases, 123 had extra-nodal NK/T-cell lymphoma (ENKTL), 12 had aggressive NK-cell leukemia (ANKL) and 34 had EBV+ T-cell lymphoma of childhood (EBV+TL). The mutation profile revealed that all three subtypes of EBV+T/NK-LPDs had high mutation rates in STAT3, KMT2D, DDX3X, NOTCH1 and TET2. Target sequencing revealed that ENKTL, ANKL and EBV+TL were molecularly distinct, the mutation in nasal-ENKTL and extra-nasal-ENKTL are also different. Survival analysis revealed that ENKTL patients with gene mutations or loss of protein expression in either KMT2D or TET2 were significantly correlated with shorter overall survival. And although the EBV+TL and ANKL groups were too small to confirm survival disadvantage, the adverse prognosis trends of KMT2D or TET2 were showed in these two groups. We conclude that EBV+T/NK lymphoproliferative disorders have very distinct molecular profiles. Our findings also suggest the likely involvement of KMT2D and TET2 in the development of ENKTL, and possibly EBV+T/NK-LPDs in general.


Assuntos
Proteínas de Ligação a DNA/genética , Infecções por Vírus Epstein-Barr/complicações , Células Matadoras Naturais/metabolismo , Células Matadoras Naturais/patologia , Transtornos Linfoproliferativos/etiologia , Transtornos Linfoproliferativos/mortalidade , Mutação , Proteínas de Neoplasias/genética , Proteínas Proto-Oncogênicas/genética , Biomarcadores , Biologia Computacional/métodos , Diagnóstico Diferencial , Suscetibilidade a Doenças , Infecções por Vírus Epstein-Barr/virologia , Feminino , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Imuno-Histoquímica , Transtornos Linfoproliferativos/diagnóstico , Transtornos Linfoproliferativos/terapia , Masculino , Anotação de Sequência Molecular
16.
Am J Surg Pathol ; 43(12): 1611-1621, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31305266

RESUMO

Chronic active Epstein-Barr virus (EBV) infection of T-cell and NK-cell type, systemic form (CAEBV-T/NK-S) is characterized by EBV T-cell and/or NK-cell proliferation with no changes suggesting malignancy. Therefore, when Hodgkin/Reed-Sternberg (HRS)-like cells are scattered in CAEBV-T/NK-S, it is more likely to be misdiagnosed as classic Hodgkin lymphoma. We encountered a case wherein the patient showed HRS-like cells with typical NK phenotype. Therefore, we further investigated 8 similar cases to provide clinicopathologic and genetic features and discuss their distinction from other related diseases. Clinically, all cases met the diagnostic criteria of CAEBV. Moreover, 4/8 patients had hemophagocytic lymphohistiocytosis. The median survival was 16 months (range, 5 to 35 mo). Pathologically, all lymph node samples had a remarkably similar morphology with scattered HRS-like cells surrounded by a mixture of small-sized lymphocytes, plasma cells, and macrophages that masqueraded classic Hodgkin lymphoma. Besides, erythrophagocytosis was detected in 4/11 samples. The HRS-like cells were positive for CD2, CD3p, CD30, CD56, GrB, and EBER-ISH, but negative for CD20, CD5, PAX-5, and LMP-1. The surrounding lymphocytes were mainly CD8 cytotoxic T cells, without obvious aberrant expression. In addition, all patients were polyclonal in the T-cell receptor γ rearrangement test. The harbored mutations were mainly in epigenetic modifiers, JAK-STAT signaling pathway, and apoptosis/cell cycle pathway, including SOCS1, DDX3X, and KMT2D, similar to other EBV-associated T/NK-cell lymphoproliferative disorders. Therefore, the evidence indicates that "HRS-like cells of NK phenotype" is a variant of CAEBV-T/NK-S. This study may raise awareness of such confounding CAEBV-T/N-S cases in clinical practice to avoid misdiagnosis and treatment delay.


Assuntos
Infecções por Vírus Epstein-Barr/diagnóstico , Rearranjo Gênico da Cadeia gama dos Receptores de Antígenos dos Linfócitos T , Doença de Hodgkin/diagnóstico , Células Matadoras Naturais/patologia , Transtornos Linfoproliferativos/diagnóstico , Mutação , Células de Reed-Sternberg/patologia , Linfócitos T/patologia , Adolescente , Adulto , Idoso , Diagnóstico Diferencial , Infecções por Vírus Epstein-Barr/genética , Infecções por Vírus Epstein-Barr/patologia , Infecções por Vírus Epstein-Barr/virologia , Feminino , Predisposição Genética para Doença , Doença de Hodgkin/genética , Doença de Hodgkin/patologia , Doença de Hodgkin/virologia , Humanos , Células Matadoras Naturais/virologia , Transtornos Linfoproliferativos/genética , Transtornos Linfoproliferativos/patologia , Transtornos Linfoproliferativos/virologia , Masculino , Pessoa de Meia-Idade , Fenótipo , Valor Preditivo dos Testes , Células de Reed-Sternberg/virologia , Linfócitos T/virologia
17.
BMC Cancer ; 19(1): 514, 2019 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-31142285

RESUMO

BACKGROUND: Crystal-storing histiocytosis (CSH) is a rare lesion characterized by sheets of crystal-laden non-neoplastic histiocytes. CSH shows a prominent association with lymphoproliferative disorders that express monoclonal immunoglobulins, mainly multiple myeloma (MM), lymphoplasmacytic lymphoma (LPL) and monoclonal gammopathy of undetermined significance (MGUS). However, no aggressive B cell lymphoma has been reported to be associated with CSH. CASE PRESENTATION: A 74-year-old Chinese woman presented with multiple subcutaneous masses, abdominal pain, and fever. An IgM kappa type of monoclonal gammopathy (MG) was noted by immunofixation performed on the patient's serum. Computed tomographic (CT) scan revealed subcutaneous masses on the left upper arm and at the waist and multiple low-density lesions in the spleen. Microscopically, sections of subcutaneous masses revealed sheets of large polygonal and spindle cells with abundant eosinophilic cytoplasm, round to ovoid eccentric nuclei, reticulate chromatin, and median nucleoli. Massive needle-shaped crystals were confined to the cytoplasm. Immunohistochemically, these crystal-containing cells were positive for CD68/PGM1, CD163, IgM, and Igκ. Meanwhile, the splenic tumour was diagnosed as diffuse large B-cell lymphoma (DLBCL), non-germinal-centre B (non-GCB) subtype (Hans algorithm). Immunohistochemistry for IgM was positive in the cytoplasm of some neoplastic cells. Immunoglobulin heavy chain (IgH) gene rearrangement was detected by PCR analysis of the subcutaneous mass and the splenic tumour. CONCLUSION: To the best of our knowledge, this is the first case of generalized CSH with DLBCL and MG. Although the rarity of CSH and separate locations of CSH and lymphoma led to a diagnostic dilemma, the presence of MG was a clue to appreciate the relation between CSH and DLBCL. This case stressed a full investigation into the underlying lymphoproliferative disorder for integrated diagnosis and correct treatments.


Assuntos
Histiocitose de Células de Langerhans/diagnóstico por imagem , Linfoma Difuso de Grandes Células B/diagnóstico por imagem , Paraproteinemias/diagnóstico por imagem , Idoso , Feminino , Rearranjo Gênico , Histiocitose de Células de Langerhans/genética , Histiocitose de Células de Langerhans/metabolismo , Humanos , Cadeias Pesadas de Imunoglobulinas/genética , Linfoma Difuso de Grandes Células B/genética , Linfoma Difuso de Grandes Células B/metabolismo , Paraproteinemias/genética , Paraproteinemias/metabolismo , Tomografia Computadorizada por Raios X
18.
Am J Transl Res ; 11(4): 1938-1947, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31105809

RESUMO

To investigate endoplasmic reticulum (ER) stress reactions in spinal cord injury rats by evaluating the expression of the glucose-regulated protein 78 (GRP78), C/EBP homologous transcription factor protein (CHOP), X-box binding protein 1 (XBP1), Eif-2α and Bad. SCI models were established using adult female mice. After SCI, the expression of endoplasmic reticulum stress-induced apoptosis proteins were examined in the mice at specific time points using immunohistochemistry and western blot. The results of immunohistochemistry showed that in spinal cord gray matter, Chop, Grp78, XBP1, Eif-2α and Bad were specifically detected in the cytoplasm of the cell. Compare with the SCI group, there was little expression in normal group and sham group. The expression of ER stress-induced apoptosis proteins were significantly increased after spinal cord injury, and the absolute expression was higher than normal group (P < 0.05). Western-Blot results showed that compare with the SCI group, there were little expression of ER stress-induced apoptosis proteins in normal group and sham group. The expression of ER stress-induced apoptosis proteins were significantly increased after spinal cord injury, and the absolute expression was higher than normal group (P < 0.05). These results suggest that some ER stress-induced apoptosis proteins, such as Chop, Grp78, XBP1, Eif-2α and Bad, were activated after spinal cord injury, but the precise regulatory mechanisms remain unclear. In the future, understanding of the precise mechanism of ER stress-mediated apoptosis in SCI may lead to the development of novel treatment strategies.

19.
Am J Surg Pathol ; 43(7): 995-1004, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31045893

RESUMO

Extranodal natural killer/T-cell lymphoma, nasal-type (ENKTL-N) initially presented in larynx is a rare condition without distinctive clinicopathologic features, with a challenging pathologic diagnosis. This study aimed to evaluate the clinicopathologic features and diagnosis of laryngeal ENKTL-N and spread awareness regarding ENKTL-N. A series of 31 cases of laryngeal ENKTL in one Chinese institution over a 9-year interval was retrospectively analyzed. Median age was 50 years (range, 13 to 77 y) with a male/female ratio of 5.2:1 (26/5). All patients initially presented with hoarseness and/or laryngalgia, and 10 patients (32.3%) experienced B symptoms. The supraglottic region was the most common site of occurrence (58.1%), the glottic area being the rarest site (6.5%). The mucosal squamous epithelium was detected in 26 specimens and pseudoepitheliomatous hyperplasia was observed in 8 cases (8/26, 30.8%). "Keratin-pearls" and a "pseudoinvasive" pattern were observed in 2 cases. Follow-up data were available for 26 patients (83.9%), the median survival duration was 9 months, and the overall survival rate at 5 years was 29.6%. Univariate analysis revealed that patients experiencing B symptoms (P=0.019) and age above 60 years had a significantly low survival (P=0.049) and that combined radiotherapy and chemotherapy prolongs overall survival (P<0.001). Laryngeal ENKTL-N is a rare entity with high aggressiveness and a poor prognosis. Multiple biopsies are usually required owing to secondary infection and massive necrosis. Laryngeal EKTL-N may mimic inflammatory lesions or well-differentiated squamous cell carcinoma. Therefore, clinical vigilance is essential to prevent misdiagnosis or a delayed diagnosis.


Assuntos
Neoplasias Laríngeas/patologia , Linfoma Extranodal de Células T-NK/patologia , Adolescente , Adulto , Idoso , Biomarcadores Tumorais/análise , Biomarcadores Tumorais/genética , China , Diagnóstico Diferencial , Feminino , Herpesvirus Humano 4/genética , Humanos , Neoplasias Laríngeas/mortalidade , Neoplasias Laríngeas/terapia , Neoplasias Laríngeas/virologia , Linfoma Extranodal de Células T-NK/mortalidade , Linfoma Extranodal de Células T-NK/terapia , Linfoma Extranodal de Células T-NK/virologia , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Prognóstico , Estudos Retrospectivos , Fatores de Risco , Fatores de Tempo
20.
Int J Clin Exp Pathol ; 12(9): 3408-3416, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31934184

RESUMO

In recent years, many studies have focused on the novel esophageal cancer-related gene 2 (ECRG2), which might be important in esophageal cancer development. The aim of this study is to investigate the relationship between ECRG2 short tandem repeat (STR) polymorphism and susceptibility to esophageal squamous cell carcinomas (ESCCs) in Kazakhs and Hans in Xinjiang. ECRG2 genotypes were detected by PCR-SSCP in 178 cases of esophageal carcinomas and 153 blood samples from the Kazakh and Han population. In Kazakhs and Hans, the frequencies of ECRG2 STR genotypes TCA3/TCA3, TCA4/TCA4, and TCA3/TCA4 were 47.8%/8.7%, 43.5%/67.9%, and 7.1%/25.0% in esophageal carcinomas with metastasis, respectively; and 14.1%/38%, 47.9%/14.3%, and 44.6%/41.1% in carcinomas without metastasis, respectively. A significant difference was observed between the groups with metastasis and without metastasis (Kazakh: χ2=13.77, P<0.01; Han: χ2=26.183, P<0.01). Compared with patients who carried the TCA4/TCA4 genotype, those who carried the TCA3/TCA3 genotype were at an increased risk of ESCC, with the adjusted odds ratios being 4.06 (95% confidence interval (CI), 1.69-9.74) in Kazakhs and 3.25 (95% CI, 1.25-8.45) in Hans. Our findings suggested that subjects who carried the TCA3/TCA3 genotype are at an increased risk of ESCC and metastasis compared with those who carried the TCA4/TCA4 genotype.

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