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1.
J Clin Anesth ; 62: 109742, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32088534

RESUMO

STUDY OBJECTIVES: The aim of the present study was to assess the quality of recovery from anesthesia on patients subjected to laparoscopic radical resection of colorectal cancer under epidural block combined with general anesthesia or general anesthesia by means of Quality of Recovery-15 (QoR-15) questionnaire. DESIGN: Prospective randomized trial. SETTING: The setting is at an operating room, a post-anesthesia care unit, and a hospital ward. PATIENTS: Seventy patients, aging from 65 to 79 years with an American Society of Anesthesiologists physical status II or III, were scheduled to undergo laparoscopic radical resection of colorectal cancer. INTERVENTIONS: Epidural block combined with general anesthesia or general anesthesia. MEASUREMENTS: The QoR-15 was administered by an investigator blind to group allocation before surgery (T0), at 24 and 72h after surgery (T1 and T2), and on postoperative day 7 (T3). The quality of recovery, as assessed by the score on the QoR-15, was compared between the groups. Besides, the consumption of anesthetics, respiratory recovery time, response time, extubation time, flatus time, the incidence of nausea or vomiting, the consumption of antiemetic and analgesic agents, and the duration of the hospital stay were also recorded. MAIN RESULTS: The QoR-15 scores at T1 and T2 were significantly higher in the E + G group compared with the G group (P < 0.05). Among the five dimensions of the QoR-15, physiological comfort, physiological independence, pain, and emotional dimension were significantly better at T1 in the E + G group, and physiological comfort and pain were significantly better at T2 in the E + G group. CONCLUSION: This study demonstrates that epidural block combined with general anesthesia can improve the early recovery of elderly patients after laparoscopic radical resection of colorectal cancer from the perspective of patients.

2.
Chem Commun (Camb) ; 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32040110

RESUMO

Here, a hollowed-out Au@AgPt core-frame nanostructure is carved in the presence of PtCl62- via galvanic replacement (GR) reaction, during which the dissolution of Ag atoms from the {100} facets and the deposition of Pt atoms on the active edges of the nanocubes occur. Both ex situ and in situ monitoring of the plasmonic and structural evolutions at the single-particle level, confirmed also by theoretical simulations, shows a three-phase mechanism involved.

3.
Ann Anat ; 227: 151418, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31626903

RESUMO

The present study aims to provide anatomical evidence for clinical application of the medial sural artery perforator (MSAP) flap. The current study investigated the vascular anatomy of the flap, evaluated the postoperative appearance and function of the donor and recipient sites, and investigate the clinical value in reconstruction of oral cavity. Six lower limbs of Chinese adult cadavers were microsurgically dissected. The locations and courses of the medial sural artery perforators were identified and recorded, which provided an anatomical basis for clinical application. Then, 16 clinical cases employing this flap were evaluated, ranging from 3×4cm to 6×8cm, and were employed for defects in the oral cavity region. Sixteen clinical cases with intraoral soft tissue defects, which included four clinical cases with inner cheek defects, were successfully followed up for 10-47 months (24 months on average). The donor site function, contour of recipient site and oral function recovery were evaluated as acceptable or better in cases with intraoral soft tissue defect, which were further verifying the value of clinical application of MSAP in repairing oral cavity defects. Moreover, two typical clinical cases were described in detail. To conclude, the MSAP flap is a favorable choice for small- to medium-size defects based on minor donor site morbidity, satisfactory oral function recovery, perforator stability and adaptation of the pedicle for anastomosis in the oral cavity region.

4.
Anal Chem ; 92(2): 2130-2135, 2020 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-31850751

RESUMO

Plasmon resonance energy transfer (PRET), as a new form of energy transfer first discovered in 2007, has been widely applied for the biomolecular recognition, detection of ions, cellular physiological status monitoring, and energy conversion. It occurs between noble metal nanoparticles (donor) and conjugated molecules or nanoparticles (acceptor). In this study, we used urchin-like gold nanoplasmonics (UGPs) and oxTMB as a new donor-acceptor pair to establish a novel PRET coupling system, avoiding trivial modification. PRET from UGPs to conjugated redox-active oxTMB leads to resonant quenching in the localized surface plasmon resonance (LSPR) spectra. However, when the acid phosphatase (ACP) was introduced, the hydrolyzate ascorbic acid (AA) converted from 2-phospho-l-ascorbic acid trisodium salt (AAP) could be capable of reducing oxTMB into TMB, thereby preventing the occurrence of PRET. The recovery of the scattering spectral intensity of UGPs was linearly related to the concentration of ACP in the range of 0.1 to 5.0 U/L, and the ACP with a detection limit of 0.076 U/L could be measured. In addition, this method also showed good selectivity attributed to the substrate specificity of enzyme.

5.
Nat Commun ; 10(1): 5161, 2019 11 14.
Artigo em Inglês | MEDLINE | ID: mdl-31727890

RESUMO

Purely organic room temperature phosphorescence (RTP) has attracted wide attention recently due to its various application potentials. However, ultralong RTP (URTP) with high efficiency is still rarely achieved. Herein, by dissolving 1,8-naphthalic anhydride in certain organic solid hosts, URTP with a lifetime of over 600 ms and overall quantum yield of over 20% is realized. Meanwhile, the URTP can also be achieved by mechanical excitation when the host is mechanoluminescent. Femtosecond transient absorption studies reveal that intersystem crossing of the host is accelerated substantially in the presence of a trace amount of 1,8-naphthalic anhydride. Accordingly, we propose that a cluster exciton spanning the host and guest forms as a transient state before the guest acts as an energy trap for the RTP state. The cluster exciton model proposed here is expected to help expand the varieties of purely organic URTP materials based on an advanced understanding of guest/host combinations.

6.
Anal Chem ; 91(24): 15798-15803, 2019 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-31747259

RESUMO

Owing to the sharp edge-enhanced electric field, gold triangular nanoplates (AuNPLs) exhibit strong localized surface plasmon resonance (LSPR) scattering and display as red spots with high intensity and narrow distribution under dark-field microscopy (DFM), based on which sensitive sensing applications could be developed. As a concept of proof, the inhibition effects of pyrophosphate (PPi) against the etching of AuNPLs based on Cu2+ and I- mediated is dynamically monitored. The etching proceeding of AuNPLs by copper ions and iodide ions leads to the gradually blue-shifted LSPR scattering color of AuNPLs under DFM from the original red to green, accompanied by the reduced LSPR scattering intensity. As a result of the strong affinity of PPi to copper ions, the presence of PPi makes the etching process greatly suppressed, thereby achieving sensitive detection of the PPi.

7.
J Phys Chem Lett ; 10(21): 6656-6663, 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31608641

RESUMO

Two-dimensional (2D) Dirac cone materials exhibit linear energy dispersion at the Fermi level, where the effective masses of carriers are very close to zero and the Fermi velocity is ultrahigh, only 2-3 orders of magnitude lower than the light velocity. Such Dirac cone materials have great promise in high-performance electronic devices. Herein, we have employed the genetic algorithm methods combined with first-principles calculations to propose a new 2D anisotropic Dirac cone material, an orthorhombic boron phosphide (BP) monolayer named borophosphene. Molecular dynamics simulation and phonon dispersion have been used to evaluate the dynamic and thermal stability of borophosphene. Because of the unique arrangements of B-B and P-P dimers, the mechanical and electronic properties are highly anisotropic. Of great interest is the fact that the Dirac cone of the borophosphene is robust, independent of in-plane biaxial and uniaxial strains, and can also be observed in its one-dimensional zigzag nanoribbons and armchair nanotubes. The Fermi velocities are ∼105 m/s, on the same order of magnitude as that of graphene. By using a tight-binding model, the origin of the Dirac cone of borophosphene is analyzed. Moreover, a unique feature of self-doping can be induced by the in-plane biaxial and uniaxial strains of borophosphene and the curvature effect of nanotubes, which is greatly beneficial for realizing high-speed carriers (holes). Our results suggest that the borophosphene holds great promise for high-performance electronic devices, which could promote experimental and theoretical studies for further exploring the potential applications of other 2D Dirac cone sheets.

8.
ACS Omega ; 4(12): 14886-14894, 2019 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-31552328

RESUMO

The thermal reverse flow reactor is an effective technical equipment for dealing with ventilation air methane, which has been causing a significant greenhouse effect. An experimental study on the thermal oxidation of ventilation air methane in a thermal reverse flow reactor was conducted. A mixture of domestic gas and ambient air was used to simulate ventilation air methane in the experiments, and the methane conversion efficiency was analyzed based on the concentration of combustion products determined by gas chromatography equipment. In addition, the effects of the switching time, the inlet methane concentration, the flow rate, and heat extraction were studied. The experimental results show that the reverse flow reactor system can run under a wide range of operating conditions with autothermal operation and high methane conversion. In addition, this system can even work with methane concentrations as low as 0.30% in the autothermal operation mode without NO x emission. Unlike previous studies, this study shows that the flow rate has little effect on the methane conversion rate in the cyclic steady state over a wide range of operating conditions. In addition, methane conversion and reaction zone change as the inlet methane concentration varies during the reaction process in the cyclic steady state. The combined optimization of operating parameters can effectively improve the stability of the reverse flow reactor system and methane conversion efficiency.

9.
Anal Chem ; 91(17): 11185-11191, 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31412694

RESUMO

Traditional fingerprints are usually obtained by pressing an inked finger on a paper. The inks would contaminate fingers and more importantly, these fingerprints are visible and able to be photocopied. In order to develop a smart membrane for fingerprint recording and document security, microrod assemblies of carbon quantum dots (CQDs)-Eu (III) are embedded in a electrospun nanofibrous (NFs) membrane which has strong red emission under UV irradiation owing to aggregation induced Dexter energy transfer from CQDs to Eu (III) ions. A clear blue emission fingerprint could be recorded on the membrane after a finger touch because the phosphate (Pi) secreted through sweat glands blocks the solid-state Dexter energy transfer, recovering the UV-irradiated blue emissions of CQDs. The Pi-based fingerprint on the membrane, which is invisible under daylight and could not be photocopied, greatly improves the security of the fingerprint and, furthermore, has the capability to identify the people who touched the secret document through the fingerprint analysis, showing that the intelligent NFs membrane can be applied for both fingerprint security and document counterspy.

10.
Talanta ; 201: 280-285, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31122424

RESUMO

Developing rapid, sensitive and intelligent optical probes is important for the growing need of microscope imaging analysis. Herein, we proposed a new strategy to assemble plasmonic nanoprobes in situ for dark-field microscopy imaging scatterometry by making use of the formation, disruption, and re-formation of cytosine-Ag+-cytosine (CAg+C) bonds. The CAg+C bond was formed at first through Ag+-mediated base pairing between C-contained aptamer and its C-mismatched complementary DNA. Owing to the subsequent binding of target with the aptamer, the CAg+C structure was disrupted, leading Ag+ to be quantitatively released. The released Ag+ ions can make the CAg+C bonds formed again between the C-contained sequence that modified gold nanospheres (AuNSs), and AuNS clusters thus formed in situ, which have strong plasmonic scattering signals owing to the coupling of localized surface plasmon resonance (LSPR). Therefore, the plasmonic scattering signals enhanced following the off-on mode under the dark field microscope from the 'zero' background to on. As a concept of proof, sensitive detections for Aflatoxin B1 (AFB1) in foods, carcinoembryonic antigen (CEA) in blood serum, and Ricin B in artificial sample, was successfully made by using of the in situ formed AuNS clusters, demonstrating that the newly developed metal-mediated strategy for assembling nanoprobes are universal.

11.
Nano Lett ; 19(4): 2272-2279, 2019 04 10.
Artigo em Inglês | MEDLINE | ID: mdl-30829039

RESUMO

RNA interference (RNAi) is demonstrated as one of the most powerful technologies for sequence-specific suppression of genes in disease therapeutics. Exploration of novel vehicles for small interfering RNA (siRNA) delivery with high efficiency, low cytotoxicity, and self-monitoring functionality is persistently pursued. Herein, by taking advantage of aggregation-induced emission luminogen (AIEgen), we developed a novel class of Ag@AIE core@shell nanocarriers with regulable and uniform morphology. It presented excellent efficiencies in siRNA delivery, target gene knockdown, and cancer cell inhibition in vitro. What's more, an anticancer efficacy up to 75% was achieved in small animal experiments without obvious toxicity. Attributing to the unique AIE properties, real-time intracellular tracking of siRNA delivery and long-term tumor tissue imaging were successfully realized. Compared to the commercial transfection reagents, significant improvements were obtained in biocompatibility, delivery efficiency, and reproducibility, representing a promising future of this nanocarrier in RNAi-related cancer therapeutics.


Assuntos
Técnicas de Transferência de Genes , Nanopartículas/administração & dosagem , Neoplasias/terapia , RNA Interferente Pequeno/administração & dosagem , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Técnicas de Silenciamento de Genes , Humanos , Nanopartículas/química , Neoplasias/genética , Neoplasias/patologia , Interferência de RNA , RNA Interferente Pequeno/química , RNA Interferente Pequeno/genética
12.
Chem Commun (Camb) ; 54(95): 13359-13362, 2018 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-30419077

RESUMO

Clear doughnut-shaped far-field scattering patterns (DNSPs) for gold regular hexagonal nanoplates (AuHNPs) larger than 500 nm (distance between the two opposite edges) have been observed by dark-field microscopy imaging. This phenomenon was attributed to the edge effect, which can be further applied for reaction process monitoring.

13.
J Genet ; 97(4): 977-985, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30262710

RESUMO

The present study aimed to identify the alternatively spliced isoforms of pig MEF2A gene and to determine theirmRNA expression patterns. Four alternatively spliced isoforms of pig MEF2A gene (i.e. MEF2A1, MEF2A2, MEF2A3 and MEF2A4) were cloned according to the results of transcriptome sequencing. The fifth to eighth exons of MEF2A1 were normally spliced. In MEF2A2, the fifth exon was missing; the sixth exon had an extra 138 bp at its 5' end, and the seventh exon had an extra 102 bp at its 3' end. In MEF2A3, the fifth exon was missing, and the sixth exon had an additional 138 bp at its 5' end. In MEF2A4, the seventh exon had an extra 102 bp at its 3' end. Quantitative real-time polymerase chain reaction (qPCR) analysis indicated that the expression profiles of the four alternatively spliced transcripts in the longissimus dorsi differed between the Mashen and Large White pigs. MEF2A1 and MEF2A2 expression levels were the highest at 90 days of age and lowest at 180 days of age. MEF2A3 and MEF2A4 expression levels increased with age (in days). The four alternatively spliced isoforms of MEF2A were also expressed in the small intestine, cerebellum, pancreas, heart and lung. The discovery of new alternatively spliced transcripts of the MEF2A gene may be utilized in understanding its biological functions.


Assuntos
Processamento Alternativo/genética , Fatores de Transcrição MEF2/genética , Suínos/genética , Animais , Regulação da Expressão Gênica/genética , Humanos , Isoformas de Proteínas/genética , Processamento de RNA/genética , RNA Mensageiro/genética
14.
ACS Nano ; 12(9): 9549-9557, 2018 09 25.
Artigo em Inglês | MEDLINE | ID: mdl-30148962

RESUMO

Sensitive and accurate detection of highly contagious virus is urgently demanded for disease diagnosis and treatment. Herein, based on a multifunctional aggregation-induced emission luminogen (AIEgen), a dual-modality readout immunoassay platform for ultrasensitive detection of viruses has been successfully demonstrated. The platform is relied on virions immuno-bridged enzymatic hydrolysis of AIEgen, accompanying with the in situ formation of highly emissive AIE aggregates and shelling of silver on gold nanoparticles. As a result, robust turn-on fluorescence and naked-eye discernible plasmonic colorimetry composed dual-signal is achieved. By further taking advantage of effective immunomagnetic enrichment, EV71 virions, as an example, can be specifically detected with a limit of detection down to 1.4 copies/µL under fluorescence modality. Additionally, semiquantitative discerning of EV71 virions is realized in a broad range from 1.3 × 103 to 2.5 × 106 copies/µL with the naked eye. Most importantly, EV71 virions in 24 real clinical samples are successfully diagnosed with 100% accuracy. Comparing to the gold standard polymerase chain reaction (PCR) assay, our immunoassay platform do not need complicated sample pretreatment and expensive instruments. This dual-modality strategy builds a good capability for both colorimetry based convenient preliminary screening and fluorescence based accurate diagnosis of suspect infections in virus-stricken areas.


Assuntos
Corantes/química , Enterovirus Humano A/metabolismo , Corantes Fluorescentes/química , Nanopartículas Metálicas/química , Vírion/metabolismo , Técnicas Biossensoriais/métodos , Cloaca/microbiologia , Enterovirus Humano A/isolamento & purificação , Ouro/química , Humanos , Hidrólise , Imunoensaio/métodos , Limite de Detecção , Faringe/microbiologia , Prata/química , Vírion/isolamento & purificação
15.
J Am Chem Soc ; 140(22): 6904-6911, 2018 06 06.
Artigo em Inglês | MEDLINE | ID: mdl-29741379

RESUMO

Multimodality imaging is highly desirable for accurate diagnosis by achieving high sensitivity, spatial-temporal resolution, and penetration depth with a single structural unit. However, it is still challenging to integrate fluorescent and plasmonic modalities into a single structure, as they are naturally incompatible because of significant fluorescence quenching by plasmonic noble-metal nanoparticles. Herein, we report a new type of silver@AIEgen (aggregation-induced emission luminogen) core-shell nanoparticle (AACSN) with both strong aggregated-state fluorescence of the AIEgen and distinctive plasmonic scattering of silver nanoparticles for multimodality imaging in living cells and small animals. The AACSNs were prepared through a redox reaction between silver ions and a redox-active AIEgen, which promoted synergistic formation of the silver core and self-assembly of the AIEgen around the core. The resulting AACSNs exhibited good biocompatibility and high resistance to environmental damage. As a result, excellent performance in fluorescence imaging, dark-field microscopy, and X-ray computed tomography-based multimodality imaging was achieved.

16.
Analyst ; 143(11): 2486-2490, 2018 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-29750225

RESUMO

Besides its widely known role as an endogenous antioxidant in scavenging free radicals, glutathione (GSH) can also play the role of prooxidant and promote CuO-induced formation of hydroxyl radicals to light up a fluorescent signal through Cu(i)-O2 chemistry without requiring additional H2O2. This approach is independent of the mechanisms of enzyme mimics, such as the well-known oxidase and peroxidase mimetics, providing a new method to simply and effectively analyze intracellular GSH.


Assuntos
Cobre/química , Fluorescência , Glutationa/química , Antioxidantes , Radicais Livres , Células HeLa , Humanos , Peróxido de Hidrogênio , Espécies Reativas de Oxigênio
17.
Chem Sci ; 8(10): 6829-6835, 2017 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-29147508

RESUMO

The Golgi apparatus is an essential subcellular organelle. Targeting and monitoring the Golgi change at the single-cell level over a long time scale are critical but are challenges that have not yet been tackled. Inspired by the precise Golgi positioning ability of galactosyltransferase and protein kinase D, due to their cysteine residues, we developed a method for long-term Golgi imaging. Fluorescent molecules, carbon quantum dots (CQDs) and silica nanoparticles could target the Golgi when they are modified with l-cysteine. l-Cysteine-rich chiral carbon quantum dots (LC-CQDs), which have the benefits of a high Golgi specificity from l-cysteine and excellent photostability and biocompatibility from the CQDs, are proven to be highly suitable for long-term in situ imaging of the Golgi. Investigation of the mechanism showed that free thiol groups and the l-type stereo configuration of LC-CQDs are essential for specific targeting of the Golgi. With the aid of the as-prepared LC-CQDs, the dynamic changes of the Golgi in the early stage of viral infection were visualized. The Golgi targeting and imaging strategy used in this work is beneficial for Golgi-targeted drug delivery and early diagnosis and therapy of Golgi diseases.

18.
Phys Chem Chem Phys ; 19(47): 31796-31803, 2017 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-29170767

RESUMO

A traditional doping technique plays an important role in the band structure engineering of two-dimensional nanostructures. Since electron interaction is changed by doping, the optical and electrochemical properties could also be significantly tuned. In this study, density functional theory calculations have been employed to explore the structural stability, and electronic and optical properties of B-doped phosphorene. The results show that all B-doped phosphorenes are stable with a relatively low binding energy. Of particular interest is that these B-doped systems exhibit an indirect band gap, which is distinct from the direct one of pure phosphorene. Despite the different concentrations and configurations of B dopants, such indirect band gaps are robust. The screened hybrid density functional HSE06 predicts that the band gap of B-doped phosphorene is slightly smaller than that of pure phosphorene. Spatial charge distributions at the valence band maximum (VBM) and the conduction band minimum (CBM) are analyzed to understand the features of an indirect band gap. By comparison with pure phosphorene, B-doped phosphorenes exhibit strong anisotropy and intensity of optical absorption. Moreover, B dopants could enhance the stability of Li adsorption on phosphorene with less sacrifice of the Li diffusion rate. Our results suggest that B-doping is an effective way of tuning the band gap, enhancing the intensity of optical absorption and improving the performances of Li adsorption, which could promote potential applications in novel optical devices and lithium-ion batteries.

19.
Nanoscale ; 9(43): 17020-17028, 2017 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-29082397

RESUMO

A membrane-based fluorescent sensing platform is a facile, point-of-care and promising technique in chemo/bio-analytical fields. However, the existing fluorescence sensing films for cancer biomarkers have several problems, with dissatisfactory sensitivity and selectivity, low utilization of probes encapsulated in films as well as the tedious design of membrane structures. In this work, a novel fluorescence sensing platform is fabricated by bio-grafting quantum dots (QDs) onto the surface of electrospun nanofibers (NFs). The aptamer integrated into the QDs/NFs can result in high specificity for recognizing and capturing biomarkers. Partially complementary DNA-attached gold nanoparticles (AuNPs) are employed to efficiently hybridize with the remaining aptamer to quench the fluorescence of QDs by nanometal surface energy transfer (NSET) between them both, which are constructed for prostate specific antigen (PSA) assay. Taking advantage of the networked nanostructure of aptamer-QDs/NFs, the fluorescent film can detect PSA with high sensitivity and a detection limit of 0.46 pg mL-1, which was further applied in real clinical serum samples. Coupling the surface grafted techniques to the advanced network nanostructure of electrospun NFs, the proposed aptasensing platform can be easily extended to achieve sensitive and selective assays for other biomarkers.


Assuntos
Aptâmeros de Nucleotídeos/química , Técnicas Biossensoriais , Nanopartículas Metálicas , Nanofibras/química , Pontos Quânticos , Biomarcadores/análise , DNA Complementar , Fluorescência , Ouro , Humanos , Limite de Detecção , Masculino , Antígeno Prostático Específico/análise
20.
Anal Chem ; 89(16): 8484-8489, 2017 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-28732160

RESUMO

For the first time, the scattering light of noble nanoparticles was applied for the simultaneous detection of dual cancer biomarkers. Two nanoprobes with dual scattering light colors were used for the simultaneous imaging of alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA) based on the sandwich-type immunoassay. Since AFP can combine anti-AFP-modified gold nanoparticles, which have green scattering light under the dark-field microscopic imaging (iDFM) technique, while CEA can conjugate anti-CEA-immobilized silver nanoparticles, which have blue scattering light, the simultaneous determination of AFP and CEA can be achieved by separately counting the number of green and blue light spots in iDFM. The mutual interference between the detection processes of AFP and CEA in the dual detection was investigated, and a negligible interference was found when the concentration of the antigen was in the range of 0.5-10 ng/mL, indicating the practicability of the simultaneous sensitive detection of dual targets. Furthermore, AFP and CEA in serum samples were also quantified directly without additional sample pretreatment, demonstrating the potential applications of the developed method in clinical diagnosis.


Assuntos
Biomarcadores Tumorais/sangue , Antígeno Carcinoembrionário/sangue , Carcinoma Hepatocelular/sangue , Cor , Neoplasias Hepáticas/sangue , alfa-Fetoproteínas/análise , Carcinoma Hepatocelular/diagnóstico , Ouro/química , Humanos , Neoplasias Hepáticas/diagnóstico , Nanopartículas Metálicas/química , Prata/química , Espectrofotometria Ultravioleta
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