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1.
J Biochem Mol Toxicol ; : e22419, 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31702098

RESUMO

Ionic liquids (ILs) as green alternatives for volatile organic solvents are increasingly used in commercial applications. It is necessary to explore the cytotoxic mechanism of ILs to reduce the risk to human health. For this purpose, cell viability, apoptosis, cytochrome P450 3A4 (CYP3A4), glucose transporter type 2 (GLUT2), and microRNA-122 (miR-122) gene expression in HepG2 cells was evaluated after IL exposure. The results showed that ILs reduced the viability of HepG2 cells through apoptotic cell death. Moreover, ILs markedly upregulated the transcription and protein levels of CYP3A4, but did not affect the expression of GLUT2 in either messenger RNA level or protein level. Finally, ILs increased the expression of miR-122 and inhibition of miR-122 with miR-122 inhibitor blocked ILs-induced apoptosis in HepG2 cells. This finding may contribute to an increased understanding of the in vitro molecular toxicity mechanism of ILs to further understand IL-related human health risks.

2.
J Hazard Mater ; : 121518, 2019 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-31704121

RESUMO

Research interests have been recently thrust into the nonradical reactions in persulfate-based advanced oxidation processes (AOPs), whilst the underlying mechanism of the nonradical pathway remains ambiguous especially in metal-based AOPs systems. In this study, we investigated the reactivity of cuprous oxide (Cu2O) for activating peroxymonosulfate (PMS) to decompose diverse organic contaminants. Cu2O exhibited a strong catalytic dependence on the crystal morphology, and cubic Cu2O was more reactive than the octahedral and rhombic dodecahedral structures for catalytic degradation of bisphenol A with PMS. Chemical quenching tests, electron paramagnetic resonance (EPR), solvent exchange and selective oxidation experiment were corporately conducted to illustrate that Cu2O-catalyzed PMS did not produce free radicals or singlet oxygen. In contrast, a surface-confined metastable intermediate would be formed via outer-sphere interactions between PMS and Cu2O, which directly attacked the organic substrate. Such a reaction pathway is intrinsically distinct from the electron-shuttling regime in carbon (or noble metal)/persulfate systems via the conductive surface of the catalyst, and the outer-sphere interactions let the activated PMS demonstrate a higher oxidizing capacity toward organic contaminants. Therefore, this study dedicates to providing new insights into the copper-catalyzed AOPs and vital supplementary to the ongoing dialogue of the nonradical catalysis in persulfate-based oxidation.

3.
Clin Cancer Res ; 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31662330

RESUMO

PURPOSE: Chromosomal instability (CIN) is a common phenomenon in colorectal cancer (CRC), but its role and underlying cause remain unknown. We have identified that mitotic regulator microtubule-associated protein 9 (MAP9) as a critical regulator of CIN in CRC. We thus studied the effect of MAP9 loss on CRC in Map9 knockout mice and in cell lines. EXPERIMENTAL DESIGN: We generated colon epithelial-specific Map9 knockout mice and evaluated CRC development. Effect of Map9 knockout on CRC progression was determined in chemical or ApcMin/+-induced CRC. Molecular mechanism of MAP9 was determined using spectral karyotyping (SKY), microtubule assays and whole genome sequencing (WGS). Clinical significance of MAP9 was examined in 141 CRC patients. RESULTS: Spontaneous colonic tumors (9.1%) were developed in colon epithelium-specific Map9 knockout mice at 17 months, but none was observed in wildtype littermates. Map9 deletion accelerated CRC formation both in ApcMin/+ mice and azoxymethane-treated mice, and reduced survival in ApcMin/+ mice. Mechanistically, MAP9 stabilized microtubules and mediated mitotic spindle assembly. MAP9 also maintained the spindle pole integrity and protected K-fibre from depolymerization at spindle poles. MAP9 loss induced severe mitosis failure, chromosome segregation errors and aneuploidy, leading to transformation of normal colon epithelial cells. WGS confirmed enhanced CIN in intestinal tumors from Map9 knockout ApcMin/+ mice. In CRC patients, MAP9 was frequently silenced and its down-regulation was associated with poor survival. CONCLUSIONS: MAP9 is a microtubule stabilizer that contributes to spindle stability and inhibits colorectal tumorigenesis, supporting the role of MAP9 as a tumor suppressor for preventing CIN in CRC.

4.
Rev Assoc Med Bras (1992) ; 65(9): 1144-1150, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31618328

RESUMO

OBJECTIVE: In view of the high incidence of polycystic ovary syndrome (PCOS) and the unsatisfactory therapeutic effects of dimethyldiguanide or clomifene citrate alone, our study aimed to investigate the therapeutic effects of dimethyldiguanide combined with clomifene citrate in the treatment of PCOS. METHODS: A total of 79 patients with POCS and 35 healthy females were included, and endometrial biopsies were obtained. The sterol regulatory element-binding protein-1 (SREBP1) expression in endometrial tissues was detected by qRT-PCR. POC patients were randomly divided into group A (n=40) and group B (n=39). Patients in group A were treated with dimethyldiguanide combined with clomifene citrate, while patients in group B were treated with clomifene citrate alone. The number of mature follicles and cervical mucus score, follicular development rate and single follicle ovulation rate, cycle pregnancy rate, early miscarriage rate, ovulation rate, endometrial thickness, positive rate of three lines sign, follicle stimulating hormone level and luteinizing hormone level were compared between the two groups. RESULTS: The expression level of SREBP1 was higher in PCOS patients than that in the healthy control. SREBP1 expression was inhibited after treatment, while the inhibitory effects of combined treatment were stronger than those of clomifene citrate alone. Compared with clomifene citrate alone, the combined treatment improved cervical mucus score, follicle development rate, single follicle ovulation rate, endometrial thickness, positive rate of three lines sign, and follicle-stimulating hormone level. CONCLUSION: The therapeutic effect of combined treatment is better than clomifene citrate alone in the treatment of PCOS.


Assuntos
Clomifeno/uso terapêutico , Fármacos para a Fertilidade Feminina/uso terapêutico , Hipoglicemiantes/uso terapêutico , Metformina/uso terapêutico , Síndrome do Ovário Policístico/tratamento farmacológico , Adulto , Muco do Colo Uterino/efeitos dos fármacos , Clomifeno/farmacologia , Quimioterapia Combinada , Endométrio/fisiopatologia , Feminino , Fármacos para a Fertilidade Feminina/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Hipoglicemiantes/farmacologia , Metformina/farmacologia , Folículo Ovariano/efeitos dos fármacos , Indução da Ovulação , Proteína de Ligação a Elemento Regulador de Esterol 1/efeitos dos fármacos , Proteína de Ligação a Elemento Regulador de Esterol 1/genética , Adulto Jovem
5.
Dent Mater J ; 2019 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-31611496

RESUMO

This study investigated the role played by different pH environments in the deterioration of bonds between Y-TZP and Ti-6Al-4V. One hundred and thirty-five specimens were randomly assigned to one of the following storage media at 37°C: (1) distilled water, pH 6.9, DW; (2) acidic solution, pH 1-2, CS; and (3) alkaline solution, pH 10-11, KS. Shear bond strength (SBS) tests were carried out at the 4-, 14-, and 30-day storage time intervals. The morphology characteristics and elements distribution of the fracture surfaces were analyzed. CS-30 showed the lowest mean SBS and the least amount of residual cement on the Ti-6Al-4V surface after the SBS tests. Bond strength tended to decrease with increasing storage time for the acidic group. Alkaline and neutral media showed little influence on the SBS of Y-TZP to Ti-6Al-4V in 30 days. Acidic environments should be properly avoided to obtain reliable long-term bonding strength between Ti-6Al-4V and Y-TZP.

6.
Int J Oral Sci ; 11(3): 31, 2019 10 02.
Artigo em Inglês | MEDLINE | ID: mdl-31575850

RESUMO

By removing a part of the structure, the tooth preparation provides restorative space, bonding surface, and finish line for various restorations on abutment. Preparation technique plays critical role in achieving the optimal result of tooth preparation. With successful application of microscope in endodontics for >30 years, there is a full expectation of microscopic dentistry. However, as relatively little progress has been made in the application of microscopic dentistry in prosthodontics, the following assumptions have been proposed: Is it suitable to choose the tooth preparation technique under the naked eye in the microscopic vision? Is there a more accurate preparation technology intended for the microscope? To obtain long-term stable therapeutic effects, is it much easier to achieve maximum tooth preservation and retinal protection and maintain periodontal tissue and oral function health under microscopic vision? Whether the microscopic prosthodontics is a gimmick or a breakthrough in obtaining an ideal tooth preparation should be resolved in microscopic tooth preparation. This article attempts to illustrate the concept, core elements, and indications of microscopic minimally invasive tooth preparation, physiological basis of dental pulp, periodontium and functions involved in tool preparation, position ergonomics and visual basis for dentists, comparison of tooth preparation by naked eyes and a microscope, and comparison of different designs of microscopic minimally invasive tooth preparation techniques. Furthermore, a clinical protocol for microscopic minimally invasive tooth preparation based on target restorative space guide plate has been put forward and new insights on the quantity and shape of microscopic minimally invasive tooth preparation has been provided.

7.
Biochem Biophys Res Commun ; 519(4): 861-867, 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31558320

RESUMO

Several studies have demonstrated that allergic asthma can induce atherosclerosis formation in mice. Moreover, allergic asthma and atherosclerosis have been shown to be strongly associated with dyslipidemia. In this study, we investigated the underlying mechanism of allergic asthma-aggravated atherosclerosis-induced cholesterol metabolism disorder in asthmatic apoE-/- mice. We found that allergic asthma increased the expression of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) in the liver and CD36 in the aorta during the acute and advanced stages of atherosclerosis, respectively. These results indicate that cholesterol biosynthesis is increased during acute atherosclerosis and cholesterol uptake and foam cell formation is increased during advanced atherosclerosis. Simvastatin administration significantly ameliorated the aortic root lesion size of asthmatic mice and significantly decreased HMGCR and CD36 expression. However, the expression of the low-density lipoprotein receptor and ATP-binding cassette transporter A1 was markedly increased, indicating that the beneficial effect of statins in allergic asthma and coronary artery disease was mediated, at least in part, by decreasing cholesterol biosynthesis and foam cell formation. In conclusion, allergic asthma aggravates atherosclerosis by regulating cholesterol metabolism in apoE-/- mice. Allergic asthma selectively promotes cholesterol biosynthesis in acute atherosclerosis and increases foam cell formation in advanced atherosclerosis.

8.
J Mech Behav Biomed Mater ; 101: 103430, 2019 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-31557660

RESUMO

Advances in adhesive technologies have increased indications for the use of inlays. Decrease in the bonded interface integrity due to wear has been cited as the main cause of its failure. However, this process of interface degradation and the influence of inlay material on damage mechanism appear to be poorly understood. Thus, we aimed to compare the wear behavior and interface damage between ceramic and resin composite inlays bonded to enamel under sliding contact and use the experimental findings to support recommendation of the appropriate inlay material. Bonded interface specimens involving tooth enamel and either ceramic or resin composite inlays were prepared and subjected to reciprocating wear tests up to 5×104 cycles. The wear track profiles and morphologies were characterized after increments of cyclic sliding contact using white light interferometry and scanning electron microscopy, respectively. Optical microscopy was used to evaluate sub-surface cracks and their propagation within the samples. A finite element analysis was used to analyze the stress distributions of the bonded interfaces. Composite inlays showed higher wear depth than the ceramic in the early stage (N ≤ 5×102 cycles), while no significant difference was found at the later stage. For ceramic inlay a greater portion of the contact load was concentrated in the ceramic structure, which facilitated cracks and chipping of the ceramic inlay, with rather minimal damage in the adjacent interface and enamel. In contrast, for the resin composite inlay there was larger stress concentrated in the adjacent enamel, which caused the development of cracks and their propagation to the inner enamel. The restoration material could contribute to the stress distribution and extent of damage within enamel-inlay bonded interfaces. A tough ceramic appears to be more effective at protecting the residual dental tissue.

9.
Rev. Assoc. Med. Bras. (1992) ; 65(9): 1144-1150, Sept. 2019. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-1041074

RESUMO

SUMMARY OBJECTIVE In view of the high incidence of polycystic ovary syndrome (PCOS) and the unsatisfactory therapeutic effects of dimethyldiguanide or clomifene citrate alone, our study aimed to investigate the therapeutic effects of dimethyldiguanide combined with clomifene citrate in the treatment of PCOS. METHODS A total of 79 patients with POCS and 35 healthy females were included, and endometrial biopsies were obtained. The sterol regulatory element-binding protein-1 (SREBP1) expression in endometrial tissues was detected by qRT-PCR. POC patients were randomly divided into group A (n=40) and group B (n=39). Patients in group A were treated with dimethyldiguanide combined with clomifene citrate, while patients in group B were treated with clomifene citrate alone. The number of mature follicles and cervical mucus score, follicular development rate and single follicle ovulation rate, cycle pregnancy rate, early miscarriage rate, ovulation rate, endometrial thickness, positive rate of three lines sign, follicle stimulating hormone level and luteinizing hormone level were compared between the two groups. RESULTS The expression level of SREBP1 was higher in PCOS patients than that in the healthy control. SREBP1 expression was inhibited after treatment, while the inhibitory effects of combined treatment were stronger than those of clomifene citrate alone. Compared with clomifene citrate alone, the combined treatment improved cervical mucus score, follicle development rate, single follicle ovulation rate, endometrial thickness, positive rate of three lines sign, and follicle-stimulating hormone level. CONCLUSION The therapeutic effect of combined treatment is better than clomifene citrate alone in the treatment of PCOS.


RESUMO OBJETIVO Tendo em vista a alta incidência de síndrome dos ovários policísticos (SOP) e os efeitos terapêuticos insatisfatórios da dimetildiguanida ou do citrato de clomifeno isoladamente, nosso estudo teve como objetivo investigar os efeitos terapêuticos da dimetildiguanida associada ao citrato de clomifeno no tratamento da SOP. MÉTODOS Um total de 79 pacientes com POCS e 35 mulheres saudáveis foram incluídos, e biópsias endometriais foram obtidas. A expressão da proteína de ligação do elemento regulador de esterol-1 (SREBP1) nos tecidos endometriais foi detectada por qRT-PCR. Pacientes POC foram divididos aleatoriamente em grupo A (n=40) e grupo B (n=39). Os pacientes do grupo A foram tratados com dimetildiguanida combinada com citrato de clomifeno, enquanto os pacientes do grupo B foram tratados apenas com citrato de clomifeno. O número de folículos maduros e muco cervical, taxa de desenvolvimento folicular e taxa de ovulação, taxa de gravidez, abortamento precoce, taxa de ovulação, espessura endometrial, taxa positiva de três linhas, nível de hormônio folículo estimulante e nível de hormônio luteinizante foram comparados entre os dois grupos. RESULTADOS O nível de expressão do SREBP1 foi maior nos pacientes com SOP do que no controle normal. A expressão de SREBP1 foi inibida após o tratamento, enquanto os efeitos inibidores do tratamento combinado foram mais fortes do que os do citrato de clomifeno isoladamente. Comparado com o citrato de clomifeno sozinho, o tratamento combinado melhorou significativamente a pontuação do muco cervical, a taxa de desenvolvimento folicular, a taxa de ovulação do folículo único, a espessura endometrial, a taxa positiva de três linhas de sinal e o nível de hormônio folículo estimulante. CONCLUSÃO O efeito terapêutico do tratamento combinado é melhor do que o citrato de clomifeno isolado no tratamento da SOP.

10.
Future Oncol ; 15(27): 3135-3148, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31426680

RESUMO

Aim: To find accurate and effective biomarkers for diagnosis of non-small-cell lung cancer (NSCLC) patients. Materials & methods: We downloaded microarray datasets GSE19188, GSE33532, GSE101929 and GSE102286 from the database of Gene Expression Omnibus. We screened out differentially expressed genes (DEGs) and miRNAs (DEMs) with GEO2R. We also performed analyses for the enrichment of DEGs' and DEMs' function and pathway by several tools including database for annotation, visualization and integrated discovery, protein-protein interaction and Kaplan-Meier-plotter. Results: Total 913 DEGs were screened out, among which ten hub genes were discovered. All the hub genes were linked to the worsening overall survival of the NSCLC patients. Besides, 98 DEMs were screened out. MiR-9 and miR-520e were the most significantly regulated miRNAs. Conclusion: Our results could provide potential targets for the diagnosis and treatment of NSCLC.

11.
Pestic Biochem Physiol ; 159: 107-117, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31400772

RESUMO

Latrophilin (LPH) is an adhesion G protein-coupled receptor (aGPCR) that participates in multiple essential physiological processes. Our previous studies have shown that lph is not only indispensable for the development and reproduction of red flour beetles (Tribolium castaneum), but also for their resistance against dichlorvos or carbofuran insecticides. However, the regulatory mechanism of lph-mediated insecticide susceptibility remains unclear. Here, we revealed that knockdown of lph in beetles resulted in opposing changes in two chemoreception genes, chemosensory protein 10 (CSP10) and odorant-binding protein C01 (OBPC01), in which the expression of TcCSP10 was downregulated, whereas the expression of TcOBPC01 was upregulated. TcCSP10 and TcOBPC01 were expressed at the highest levels in early pupal and late larval stages, respectively. High levels of expression of both these genes were observed in the heads (without antennae) of adults. TcCSP10 and TcOBPC01 were significantly induced by dichlorvos or carbofuran between 12 and 72 h (hrs) after exposure, suggesting that they are likely associated with increasing the binding affinity of insecticides, leading to a decrease in sensitivity to the insecticides. Moreover, once these two genes were knocked down, the susceptibility of the beetles to dichlorvos or carbofuran was enhanced. Additionally, RNA interference (RNAi) targeting of lph followed by exposure to dichlorvos or carbofuran also caused the opposing expression levels of TcCSP10 and TcOBPC01 compared to the expression levels of wild-type larvae treated with insecticides alone. All these results indicate that lph is involved in insecticide susceptibility through positively regulating TcCSP10; and the susceptibility could also further partially compensated for through the negative regulation of TcOBPC01 when lph was knockdown in the red flour beetle. Our studies shed new light on the molecular regulatory mechanisms of lph related to insecticide susceptibility.


Assuntos
Proteínas de Insetos/metabolismo , Inseticidas/farmacologia , Receptores de Peptídeos/metabolismo , Tribolium/efeitos dos fármacos , Tribolium/metabolismo , Animais , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Regulação da Expressão Gênica no Desenvolvimento/genética , Proteínas de Insetos/genética
12.
J Prosthodont Res ; 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31395520

RESUMO

PURPOSE: To compare the effects of airborne-particle abrasion protocols on the surface morphology, the phase transformation and the resin bond strength of highly translucent zirconia (M) and conventional zirconia (Z). METHODS: Thirteen groups (N = 12) of Z and M specimens were prepared. Except for the control group, the specimens were sandblasted with conditions involving different grit sizes (50 µm or 110 µm), treatment times (10 s or 20 s) and pressures (0.1 MPa, 0.3 MPa or 0.6 MPa). The surface morphology was analyzed using scanning electron microscope (SEM) and the phase analysis was conducted with X-ray diffraction (XRD). The Ra and the shear bond strength (SBS) were measured and statistically analyzed, and the failure mode was determined by optical microscope. RESULTS: The surface morphologies were strongly dependent on treatment conditions. Larger particle size and higher pressure resulted in higher Ra for both materials. Longer blasting time resulted in higher Ra for Z but not M. Overall, the SBS increased with increasing Ra; the highest average SBS was achieved by M and exceeded 18 MPa. The monoclinic transformation was not found in any treatment for M, but was found in Z. CONCLUSIONS: Z and M showed different dependence on the airborne-particle abrasion parameters in terms of Ra, SBS and phase transformation. The conditions for maximizing SBS included a 110 µm particle size and 20 s treatment for both, with pressures of 0.3 MPa and 0.6 MPa for the M and Z, respectively.

13.
J Cancer Res Ther ; 15(4): 766-772, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31436230

RESUMO

Background/Aim: Percutaneous thermal ablation combined with transarterial chemoembolization (TACE) becomes a treatment option for unresectable hepatocellular carcinoma (HCC). This study aims to investigate the safety and feasibility of percutaneous thermal ablation combined with simultaneous TACE for patients with HCC ≤ 5 cm. Materials and Methods: From June 2010 to February 2017, a total of 280 patients with HCC ≤ 5 cm who underwent percutaneous thermal ablation combined with simultaneous TACE were included in our study. Their clinical data were collected and analyzed. Results: Major complications occurred in five cases (1.8%). The complete necrosis rate was 91.9%. The median overall survival (OS) was 66.5 months (95% confidence interval [CI] = 57.7-75.2). The OS rates in 1-, 3-, 5-, and 7-year were 96.7%, 76.0%, 59.7%, and 31.1%, respectively. Tumor size (hazard ratio = 1.826; 95% CI = 1.131-2.947; P = 0.014) was considered as independent prognostic factors of long-term survival. Conclusion: Percutaneous thermal ablation combined with simultaneous TACE is a safe and effective treatment for HCC ≤ 5 cm.

14.
Future Oncol ; 15(30): 3457-3465, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31460788

RESUMO

Aim: To reassess the prognostic performance of the American Joint Committee on Cancer (AJCC) 8th edition for pancreatic ductal adenocarcinoma (PDAC) and optimize the categorization of PDAC staging. Patients & methods: A total of 11,858 patients with resected PDAC from the Surveillance, Epidemiology and End Results database were retrospectively enrolled by sequential analyses. Results: There was no statistical significance between stage IIA and IIB tumors with hazard ratios of 2.065 and 2.184 (p = 0.620) for stages IIA and IIB, respectively. With the proposed modification, there was a significant difference between the hazard ratios of stages IIIA and IIIB which were 2.481 and 2.715, respectively (p = 0.009). The C-index of modified system was 0.609, slightly higher than AJCC 8th staging system 0.604. Conclusion: We proposed a modified eighth edition of the AJCC staging system by combining stage IIA with IIB and further subclassifying stage III patients in order to lead to better discriminative power.

15.
Int J Nanomedicine ; 14: 6135-6150, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31447556

RESUMO

Background: Nanostructured lipid carriers (NLCs) are emerging as attractive drug carriers in transdermal drug delivery. The surface modification of NLCs with cell-penetrating peptides (CPPs) can enhance the skin permeation of drugs. Purpose: The objective of the current study was to evaluate the ability of the cell-penetrating peptide (CPP) polyarginine to translocate NLCs loaded with lornoxicam (LN) into the skin layers and to evaluate its anti-inflammatory effect. Methods: The NLCs were prepared using an emulsion evaporation and low temperature solidification technique using glyceryl monostearates, triglycerides, DOGS-NTA-Ni lipids and surfactants, and then six histidine-tagged polyarginine containing 11 arginine (R11) peptides was modified on the surface of NLCs. Results: The developed NLCs formulated with LN and R11 (LN-NLC-R11) were incorporated into 2% HPMC gels. NLCs were prepared with a particle size of (121.81±3.61)-(145.72±4.78) nm, and the zeta potential decreased from (-30.30±2.07) to (-14.66±0.74) mV after the modification of R11 peptides. The encapsulation efficiency and drug loading were (74.61±1.13) % and (7.92±0.33) %, respectively, regardless of the surface modification. Cellular uptake assays using HaCaT cells suggested that the NLC modified with R11 (0.02%, w/w) significantly enhanced the cell internalization of nanoparticles relative to unmodified NLCs (P<0.05 or P<0.01). An in vitro skin permeation study showed better permeation-enhancing ability of R11 (0.02%, w/w) than that of other content (0.01% or 0.04%). In carrageenan-induced rat paw edema models, LN-NLC-R11 gels inhibited rat paw edema and the production of inflammatory cytokines compared with LN-NLC gels and LN gels (P<0.01). Conclusion: In our investigation, it was strongly demonstrated that the surface modification of NLC with R11 enhanced the translocation of LN across the skin, thereby alleviating inflammation.


Assuntos
Portadores de Fármacos/química , Sistemas de Liberação de Medicamentos , Edema/tratamento farmacológico , Lipídeos/química , Nanoestruturas/química , Peptídeos/farmacologia , Piroxicam/análogos & derivados , Administração Cutânea , Animais , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Carragenina , Morte Celular/efeitos dos fármacos , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Peptídeos Penetradores de Células/farmacologia , Edema/induzido quimicamente , Edema/metabolismo , Emulsões/química , Endocitose/efeitos dos fármacos , Géis/química , Humanos , Camundongos Endogâmicos BALB C , Nanopartículas/química , Nanopartículas/ultraestrutura , Oligopeptídeos/farmacologia , Oligopeptídeos/uso terapêutico , Piroxicam/administração & dosagem , Piroxicam/farmacologia , Piroxicam/uso terapêutico , Coelhos , Ratos Sprague-Dawley , Pele/efeitos dos fármacos , Absorção Cutânea/efeitos dos fármacos , Testes de Irritação da Pele
16.
Adv Mater ; 31(37): e1903013, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31347215

RESUMO

Silicon-based biomaterials play an indispensable role in biomedical engineering; however, due to the lack of intrinsic functionalities of silicon, the applications of silicon-based nanomaterials are largely limited to only serving as carriers for drug delivery systems. Meanwhile, the intrinsically poor biodegradation nature for silicon-based biomaterials as typical inorganic materials also impedes their further in vivo biomedical use and clinical translation. Herein, by the rational design and wet chemical exfoliation synthesis of the 2D silicene nanosheets, traditional 0D nanoparticulate nanosystems are transformed into 2D material systems, silicene nanosheets (SNSs), which feature an intriguing physiochemical nature for photo-triggered therapeutics and diagnostic imaging and greatly favorable biological effects of biocompatibility and biodegradation. In combination with DFT-based molecular dynamics (MD) calculations, the underlying mechanism of silicene interactions with bio-milieu and its degradation behavior are probed under specific simulated physiological conditions. This work introduces a new form of silicon-based biomaterials with 2D structure featuring biodegradability, biocompatibility, and multifunctionality for theranostic nanomedicine, which is expected to promise high clinical potentials.

17.
Int J Biol Macromol ; 138: 819-830, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31351959

RESUMO

The effects of dielectric barrier discharge (DBD) plasma treatment on physicochemical and digestion properties of Tartary buckwheat (TBS), sorghum (SS), wheat (WS) and quinoa (QS) starches were investigated. Plasma treatment resulted in higher solubility and lower swelling capacity of the starch samples studied. Through XRD and FTIR analyses, the relative crystallinity of starch was increased by DBD plasma, while the crystalline type remained unchanged. It was observed from SEM that more fissures and holes appeared for TBS and SS granules, and granule aggregation occurred for WS and QS caused by plasma treatment, both of which made digestion enzymes more accessible to the four starches. In addition, a marked increase in gelatinization temperature and significant reduction in viscosity of plasma treated starches were found. Interestingly, the increased enzyme accessibility and decreased viscosity of starch were consistent with the enhanced starch digestibility that was suggested by the increased RDS content, digestion rate and digestion velocity constant. Therefore, the DBD plasma treatment on starch may be not in favor of postprandial blood sugar control, but it is suggested to be applied in some industrial processes that need acceleration in starch hydrolyzation, such as bioethanol production, brewing and food fermentation.

18.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 37(3): 229-235, 2019 Jun 01.
Artigo em Chinês | MEDLINE | ID: mdl-31218853

RESUMO

Tooth preparation is the primary and core operation technique for dental esthetic restoration treatment, due to its effect of providing restoration space, bonding interfaces and marginal lines for dental rehabilitation after tooth tissue reduction. The concept of microscopic minimal invasive dentistry put forward the issue of conducting high-quality tooth preparation, conserve tooth-structure, protect vital pulp and periodontal tissue simultaneously. This study reviewed the concepts, physiology background, design and minimal invasive microscopic tooth preparation, and in the meantime, individualized strategies and the two core elements of tooth preparation (quantity and shape) are listed.


Assuntos
Porcelana Dentária , Estética Dentária , Preparo do Dente , Restauração Dentária Permanente
19.
Atherosclerosis ; 286: 128-134, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31154080

RESUMO

BACKGROUND AND AIMS: Allergic asthma can accelerate atherosclerosis, a disease in which plaque is deposited onto arterial walls and that may lead to coronary artery disease (CAD). Eosinophils are the most important effector cells in allergic asthma and are likely to become novel biomarkers for risk stratification of patients with CAD, but the relationship between eosinophil count and CAD remains unclear. We aimed to evaluate this relationship and the use of eosinophils in predicting CAD. METHODS: A total of 5287 patients who underwent coronary angiography were recruited. Their biochemical parameters, including eosinophil count, were measured and their correlation with the severity of coronary artery stenosis, as quantified by the Gensini score system, was evaluated. RESULTS: The percentages of eosinophils in leukocytes (PELs) were lower in CAD patients (p < 0.001), and had a significant negative correlation with Gensini scores (r = -0.112, p < 0.001). PELs were also significantly lower in acute myocardial infarction patients (p < 0.001). After adjusting for baseline differences, low PELs remained strongly associated with severe CAD and acute coronary arterial thrombotic event. Receiver-operating characteristic curve analysis showed that combining PELs with traditional risk factors in predictive models for CAD severity (z = 4.470, p < 0.001) or acute coronary arterial thrombotic event (z = 9.435, p < 0.001) improved the predictive capabilities of those models. CONCLUSIONS: PELs, at least in patients undergoing coronary angiography, may be strongly related to the subtype and severity of CAD and, therefore, eosinophil count may be an accurate and independent biomarker to predict CAD severity and acute coronary arterial thrombotic events.

20.
Cancer Lett ; 460: 42-53, 2019 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-31233838

RESUMO

Pancreatic ductal adenocarcinoma (PDAC) remains a refractory disease. Programmed cell death protein-1 (PD-1) monotherapy has shown strong performance in targeting several malignancies. However, the effect and mechanism of intrinsic PD-1 in pancreatic cancer cells is still unknown. In this study, associations between clinicopathological characteristics and stained tissue microarrays of PDAC specimens were analyzed along with profiling and functional analyses. The results showed that cell-intrinsic PD-1 was significantly correlated with overall survival (OS). Independently of adaptive immunity, intrinsic PD-1 promoted tumor growth in PDAC. Concomitantly, the overexpression of intrinsic PD-1 enhanced cancer proliferation and inhibited cell apoptosis in vitro and in vivo. Mechanistically, PD-1 binds to the downstream MOB1, thereby inhibiting its phosphorylation. Moreover, greater synergistic tumor suppression in vitro resulted from combining Hippo inhibitors with anti-PD-1 treatment compared with the suppression achieved by either single agent alone. Additionally, Hippo downstream targets, CYR61 (CCN1) and CTGF (CCN2), were directly affected by PD-1 mediated Hippo signaling activation in concert with survival outcomes. Finally, the formulated nomogram showed superior predictive accuracy for OS in comparison with the TNM stage alone. Therefore, PD-1 immunotherapy in combination with Hippo pathway inhibitors may optimize the anti-tumor efficacy in PDAC patients via targeting cell-intrinsic PD-1.

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