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1.
J Ovarian Res ; 14(1): 164, 2021 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-34789292

RESUMO

PURPOSE: STAT3 and p-STAT3 are often overexpressed in various human tumours and participate in cancer development and progression. However, whether STAT3/p-STAT3 expression is associated with clinicopathologic characteristics and has prognostic significance for people suffering from ovarian cancer remains controversial. We conducted a systematic review and meta-analyses to clarify the associations between STAT3/p-STAT3 expression and clinicopathologic characteristics and prognostic factors of ovarian cancer. METHODS: A systematic electronic search in the PubMed, Embase, CNKI, and Wanfang databases was conducted to identify relevant articles published before 3 April 2021. All statistical analyses were performed using Stata 15.1. RESULTS: We included 16 eligible studies incorporating 1747 ovarian cancer patients. The expression of STAT3/p-STAT3 was upregulated in ovarian cancer samples versus normal ovarian tissue, benign tumours and borderline tumours (OR = 10.14, p < 0.00001; OR = 9.08, P < 0.00001; OR = 4.01, p < 0.00001, respectively). STAT3/p-STAT3 overexpression was significantly correlated with FIGO stage (I-II vs. III-IV) (OR = 0.36, p < 0.00001), tumour grade (G1 + G2 vs. G3) (OR = 0.55; p = 0.001) and lymph node metastasis (yes vs. no) (OR = 3.39; p < 0.00001). High STAT3/p-STAT3 expression was correlated with poorer prognosis of ovarian cancer patients for both overall survival (OS) (HR = 1.67, p < 0.00001) and progression-free survival (PFS) (HR = 1.40, p = 0.007). CONCLUSION: The present meta-analysis indicated that high STAT3/p-STAT3 expression is likely predictive of an unfavourable prognosis in ovarian cancer patients. Nonetheless, prospective trials are required to confirm these associations.

2.
Int Endod J ; 2021 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-34807471

RESUMO

AIM: To evaluate the effects of hsa-miRNA-143-3p on the cytodifferentiation of human stem cells from the apical papilla (hSCAPs) and the post-transcriptional regulation of Nuclear factor I-C (NFIC). METHODOLOGY: miRNA expression profiles in human immature permanent teeth and during hSCAP differentiation were examined. hSCAPs were treated with miR-143-3p overexpression or silencing viruses, and the proliferation and odontogenic and osteogenic differentiation of these stem cells, and the involvement of the NFIC pathway, were investigated. Luciferase reporter and NFIC mutant plasmids were used to confirm NFIC mRNA as a direct target of miR-143-3p. NFIC expression analysis in the miR-143-3p overexpressing hSCAPs was used to investigate whether miR-143-3p functioned by targeting NFIC. Student's t-test and chi-square tests were used for statistical analysis. RESULTS: miR-143-3p expression was screened by microarray profiling and was found to be significantly reduced during hSCAP differentiation (p < .05). Overexpression of miR-143-3p inhibited the mineralization of hSCAPs significantly (p < .05) and downregulated the levels of odontogenic differentiation markers (NFIC [p < .05], DSP [p < .01] and KLF4 [p < .01]), whereas silencing of miR-143-3p had the opposite effect. The luciferase reporter gene detection and bioinformatic approaches identified NFIC mRNA as a potential target of miR-143-3p. NFIC overexpression reversed the inhibitory effect of miR-143-3p on the odontogenic differentiation of hSCAPs. CONCLUSIONS: miR-143-3p maintained the stemness of hSCAPs and modulated their differentiation negatively by directly targeting NFIC. Thus, inhibition of this miRNA represents a potential strategy to promote the regeneration of damaged tooth roots.

3.
J Oncol ; 2021: 4312296, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34567115

RESUMO

Background: The purpose of this study is to explore the correlation between the 18F-FDG PET/CT imaging maximum standardized uptake value (SUVmax) of preoperative colon cancer primary lesions and clinicopathological factors. Methods: 88 colon cancer patients diagnosed by histopathology were collected from January 2014 to December 2015. 18F-FDG PET/CT imaging was performed before surgery. Kaplan-Meier survival analysis was used to assess the prognosis of colon cancer patients. Results: The 18F-FDG PET/CT imaging SUVmax value of preoperative colon cancer primary lesion was significantly correlated with the length of the lesion, clinical stage, histopathological type, and the degree of tumor differentiation. The SUVmax value of tumors with long-diameter, ≥ 3 cm, clinically high-stage, adenocarcinoma, and poorly differentiated lesions was significantly high. In addition, the consistency between PET/CT and surgical pathological results at stage I and IV was higher. Stage II and III PET/CT are basically consistent with the pathological results of surgery. Kaplan-Meier survival analysis showed that the 5-year event-free survival rate of the SUVmax > 18.26 group was significantly lower than that of the SUVmax ≤ 18.26 group. Conclusion: 18F-FDG PET/CT imaging SUVmax of preoperative colon cancer primary lesions can not only reflect the proliferation and invasion ability but also monitor the recurrence and metastasis of colon cancer.

4.
Interdiscip Sci ; 2021 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-34536209

RESUMO

The severity of fundus arteriosclerosis can be determined and divided into four grades according to fundus images. Automatically grading of the fundus arteriosclerosis is helpful in clinical practices, so this paper proposes a convolutional neural network (CNN) method based on hierarchical attention maps to solve the automatic grading problem. First, we use the retinal vessel segmentation model to separate the important vascular region and the non-vascular background region from the fundus image and obtain two attention maps. The two maps are regarded as inputs to construct a two-stream CNN (TSNet), to focus on feature information through mutual reference between the two regions. In addition, we use convex hull attention maps in the one-stream CNN (OSNet) to learn valuable areas where the retinal vessels are concentrated. Then, we design an integrated OTNet model which is composed of TSNet that learns image feature information and OSNet that learns discriminative areas. After obtaining the representation learning parts of the two networks, we can train the classification layer to achieve better results. Our proposed TSNet reaches the AUC value of 0.796 and the ACC value of 0.592 on the testing set, and the integrated model OTNet reaches the AUC value of 0.806 and the ACC value of 0.606, which are better than the results of other comparable models. As far as we know, this is the first attempt to use deep learning to classify the severity of atherosclerosis in fundus images. The prediction results of our proposed method can be accepted by doctors, which shows that our method has a certain application value.

5.
Biomaterials ; 277: 121119, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34492583

RESUMO

Multi-drug resistance (MDR) is one of the leading causes of the anticancer failures. Besides the blockage of the MDR pathways, the development of more potent drugs is with urgent needs, but has been postponed mainly due to an imbalance between safety and efficacy. The recent development of the bioorthogonal prodrug activation strategy has shown immense potential to balance safety and efficacy, while recent studies only focused on few drug entities such as doxorubicin and monomethyl auristatin E, leaving the vast collection of toxins undetermined. Here we have enumerated typical molecular entities ranging from food and drug administration (FDA) approved drugs to a heated antibody drug conjugates (ADC) warhead and a trichothecene toxin to demonstrate that the bioorthogonal caging and specific activation could serve as a general design to increase the therapeutic index of bioactive molecules. These prodrugs can be efficiently activated on-demand by the bioorthogonal activators whose distribution was regulated by the cancer cell specific enzymatic non-covalent synthesis of supramolecular self-assemblies. The prodrug activation not only enhanced the synergistic therapeutic effect within a broad range of dose ratios but also allowed the convenient switching of drug identities to successfully combat MDR tumor in vivo. In general, this strategy might serve as a general platform, which can be readily applicable to enlarge the therapeutic window for various bioactive molecules. We envision that the spatiotemporal controlled bioorthogonal prodrug activation would facilitate the discovery of anticancer drugs.


Assuntos
Antineoplásicos , Neoplasias , Pró-Fármacos , Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Doxorrubicina/farmacologia , Doxorrubicina/uso terapêutico , Resistência a Múltiplos Medicamentos , Humanos , Neoplasias/tratamento farmacológico , Pró-Fármacos/uso terapêutico , Estados Unidos
6.
PLoS Genet ; 17(8): e1009693, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34351909

RESUMO

The ubiquitin-proteasome system plays important roles in various biological processes as it degrades the majority of cellular proteins. Adequate proteasomal degradation of crucial transcription regulators ensures the proper development of neutrophils. The ubiquitin E3 ligase of Growth factor independent 1 (GFI1), a key transcription repressor governing terminal granulopoiesis, remains obscure. Here we report that the deficiency of the ring finger protein Interferon regulatory factor 2 binding protein 2a (Irf2bp2a) leads to an impairment of neutrophils differentiation in zebrafish. Mechanistically, Irf2bp2a functions as a ubiquitin E3 ligase targeting Gfi1aa for proteasomal degradation. Moreover, irf2bp2a gene is repressed by Gfi1aa, thus forming a negative feedback loop between Irf2bp2a and Gfi1aa during neutrophils maturation. Different levels of GFI1 may turn it into a tumor suppressor or an oncogene in malignant myelopoiesis. Therefore, discovery of certain drug targets GFI1 for proteasomal degradation by IRF2BP2 might be an effective anti-cancer strategy.


Assuntos
Proteínas de Ligação a DNA/genética , Complexo de Endopeptidases do Proteassoma/metabolismo , Proteínas de Peixe-Zebra/metabolismo , Peixe-Zebra/crescimento & desenvolvimento , Animais , Proteínas de Ligação a DNA/metabolismo , Retroalimentação Fisiológica , Feminino , Regulação da Expressão Gênica , Técnicas de Inativação de Genes , Células HEK293 , Células HL-60 , Humanos , Leucopoese , Masculino , Proteólise , Peixe-Zebra/genética , Peixe-Zebra/metabolismo , Proteínas de Peixe-Zebra/genética
7.
Front Chem ; 9: 709581, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34336792

RESUMO

For wide applications of the lacZ gene in cellular/molecular biology, small animal investigations, and clinical assessments, the improvement of noninvasive imaging approaches to precisely assay gene expression has garnered much attention. In this study, we investigate a novel molecular platform in which alizarin 2-O-ß-d-galactopyranoside AZ-1 acts as a lacZ gene/ß-gal responsive 1H-MRI probe to induce significant 1H-MRI contrast changes in relaxation times T 1 and T 2 in situ as a concerted effect for the discovery of ß-gal activity with the exposure of Fe3+. We also demonstrate the capability of this strategy for detecting ß-gal activity with lacZ-transfected human MCF7 breast and PC3 prostate cancer cells by reaction-enhanced 1H-MRI T 1 and T 2 relaxation mapping.

8.
Mitochondrial DNA B Resour ; 6(8): 2433-2435, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34350358

RESUMO

To better understand the diversity and phylogeny of Perlodidae, we sequenced and annotated the complete mitochondrial genome (mitogenome) of Arcynopteryx dichroa. This mitogenome was 16,215 bp long and encoded 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), 2 ribosomal RNA unit genes (rRNAs), and a control region like other plecopteran. The nucleotide composition of A. dichroa mitochondrial genome obviously biases toward A and T. The A + T content of the whole mitochondrial genome, the PCGs, tRNAs, rRNAs, and the control region were: 69.3%, 67.6%, 69.8%, 71.3%, and 78.7%. Phylogenetic relationship based on the concatenated sequences of 13 PCGs and two ribosomal RNAs showed that the family Perlodidae and Chloroperlidae are sister relationship, family Perlidae being the sister group to the clade (Perlodidae + Chloroperlidae). The monophyly of the family Perlodidae is well supported but the subfamily Perlodinae and Isoperlinae are not monophyletic groups.

9.
Artigo em Inglês | MEDLINE | ID: mdl-34454086

RESUMO

Toosendanin (TSN) is a crucial component from Toosendan Fructus with a promising anti-tumor capacity. It is also the primary suspect hepatotoxic component of Toosendan Fructus. However, the mechanisms underlying TSN-induced liver injury are still largely unknown. In present study, we evaluated the hepatotoxicity of TSN on zebrafish and explored the role of inflammation, autophagy, and apoptosis in TSN-induced hepatotoxicity. We found that TSN treatment decreased the area and fluorescence intensity of zebrafish liver in time- and dose-dependent manners at nonlethal concentrations. The ALT and AST activities were increased after TSN treatment. Severe cytoplasmic vacuolation and nuclear shrank were found in the liver of TSN-treated zebrafish. The expression profile of genes demonstrated that inflammation, autophagy and apoptosis pathways were involved in TSN-induced hepatotoxicity. Our study demonstrated for the first time that TSN treatment gave rise to liver injury in zebrafish, and inflammation, autophagy, apoptosis played a role in TSN-induced hepatotoxicity.

10.
Comput Methods Programs Biomed ; 208: 106274, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34325376

RESUMO

BACKGROUND AND OBJECTIVE: Arteriosclerosis can reflect the severity of hypertension, which is one of the main diseases threatening human life safety. But Arteriosclerosis retinopathy detection involves costly and time-consuming manual assessment. To meet the urgent needs of automation, this paper developed a novel arteriosclerosis retinopathy grading method based on convolutional neural network. METHODS: Firstly, we propose a good scheme for extracting features facing the fundus blood vessel background using image merging for contour enhancement. In this step, the original image is dealt with adaptive threshold processing to generate the new contour channel, which merge with the original three-channel image. Then, we employ the pre-trained convolutional neural network with transfer learning to speed up training and contour image channel parameter with Kaiming initialization. Moreover, ArcLoss is applied to increase inter-class differences and intra-class similarity aiming to the high similarity of images of different classes in the dataset. RESULTS: The accuracy of arteriosclerosis retinopathy grading achieved by the proposed method is up to 65.354%, which is nearly 4% higher than those of the exiting methods. The Kappa of our method is 0.508 in arteriosclerosis retinopathy grading. CONCLUSIONS: An experimental study on multiple metrics demonstrates the superiority of our method, which will be a useful to the toolbox for arteriosclerosis retinopathy grading.


Assuntos
Arteriosclerose , Doenças Retinianas , Automação , Fundo de Olho , Humanos , Processamento de Imagem Assistida por Computador , Redes Neurais de Computação , Doenças Retinianas/diagnóstico por imagem
11.
Front Pharmacol ; 12: 642480, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33967776

RESUMO

Aconitine (AC), one of the bioactive diterpenoid alkaloids extracted from Aconitum plants, is widely used in traditional herbal medicine to treat various diseases. Emerging evidence indicates that AC has attracted great interest for its wide cardiotoxicity and neurotoxicity. However, the toxic effects of AC on embryonic development and its underlying mechanisms remain unclear. Here, a developmental toxicity assay of AC was performed on zebrafish embryos from 4 to 96 h post fertilization (hpf), and its underlying mechanisms were discussed. AC exposure impaired the cardiac, liver, and neurodevelopment. Especially, a high dose of AC (7.27 and 8.23 µM) exposure resulted in malformations at 72 and 96 hpf, including reduced body length, curved body shape, pericardial edema, yolk retention, swim bladder and brain developmental deficiency, and degeneration of dopaminergic neurons. High-concentration AC exposure caused a deficient cardiovascular system with cardiac dysfunctions, increased heart rates at 72 and 96 hpf, and reduced locomotor behavior at 120 hpf. AC treatment significantly increased the ROS level and triggered cell apoptosis in the heart and brain regions of embryos at 96 hpf in 7.27 and 8.23 µM AC treatment zebrafish. Oxidative stress was confirmed by reduced levels of T-SOD activity associated with accumulation of lipid peroxidation in larvae. The expression levels of oxidative stress-related genes (Nrf2, HO-1, Cat, and Sod-1) Erk1/2 and Bcl-2 were significantly downregulated at 96 hpf. The expression pattern of JNK and mitochondrial apoptosis-related genes (Bad, Bax, Cyto C, Casp-9, and Casp-3) was significantly upregulated. Taken together, all these parameters collectively provide the first evidence of AC-induced developmental toxicity in zebrafish embryo/larvae through ROS-medicated mitochondrial apoptosis involving Nrf2/HO-1 and JNK/Erk pathways.

12.
Front Endocrinol (Lausanne) ; 12: 658198, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34012420

RESUMO

Objective: Type 2 diabetes mellitus (T2DM) is often accompanied by cognitive decline and depressive symptoms. Numerous diffusion tensor imaging (DTI) studies revealed microstructural white matter (WM) abnormalities in T2DM but the findings were inconsistent. The present study aimed to conduct a coordinate-based meta-analysis (CBMA) to identify statistical consensus of DTI studies in T2DM. Methods: We performed a systematic search on relevant studies that reported fractional anisotropy (FA) differences between T2DM patients and healthy controls (HC). The anisotropic effect size seed-based d mapping (AES-SDM) approach was used to explore WM alterations in T2DM. A meta-regression was then used to analyze potential influences of sample characteristics on regional FA changes. Results: A total of eight studies that comprised 245 patients and 200 HC, along with 52 coordinates were extracted. The meta-analysis identified FA reductions in three clusters including the left inferior network, the corpus callosum (CC), and the left olfactory cortex. Besides, FA in the CC was negatively correlated with body mass index (BMI) in the patients group. Conclusions: T2DM could lead to subtle WM microstructural alterations, which might be associated with cognitive deficits or emotional distress symptoms. This provides a better understanding of the pathophysiology of neurodegeneration and complications in T2DM. Systematic Review Registration: Registered at PROSPERO (http://www.crd.york.ac.uk/PROSPERO), registration number: CRD42020218737.

13.
BMJ Open ; 11(4): e043647, 2021 04 16.
Artigo em Inglês | MEDLINE | ID: mdl-33863714

RESUMO

OBJECTIVES: Dental anxiety remains widespread among children, may continue into adulthood and affect their oral health-related quality of life and clinical management. The aim of the study was to explore the trend of children's dental anxiety over time and potential risk factors. DESIGN: Longitudinal study. METHODS: Children aged between 5 and 12 years were investigated with the Chinese version of face version of Children's Fear Survey Schedule-Dental Subscale (CFSS-DS) and Frankl Behavior Rating scale from 2008 to 2017, and influential factors were explored. RESULTS: Clinical data were available from 1061 children, including 533 (50.2%) male participants and 528 (49.8%) female participants. The total CFSS-DS scores ranged from 16 to 66, with a mean of 24.8±10.3. The prevalence of dental anxiety is 11.59%. No significant differences in total CFSS-DS scores between girls and boys were found. According to the Frankl scale, 238 children were allocated to the uncooperative group and the remaining 823 children were allocated to the cooperative group. Scores of CFSS-DS were negatively correlated with the clinical behaviour level of Frankl. Children aged 11-12 years old had significantly decreased scores compared with other age groups, and there was a decline in the scores of the group aged 8-10 years old over time. The factor analysis divided 15 items of CFSS-DS into four factors, and the total scores of 'less invasive oral procedures' items belonging to factor III decreased significantly over time in the group aged 8-10 years old. CONCLUSIONS: Age is a significant determinant for children's dental anxiety, and dental anxiety outcomes have improved for Chinese children aged 8-10 years. This study is one of the few reports on changes of children's dental anxiety in a new era of information, but the results may be extrapolated to other populations with caution.


Assuntos
Ansiedade ao Tratamento Odontológico , Qualidade de Vida , Adulto , Criança , Comportamento Infantil , Pré-Escolar , China/epidemiologia , Ansiedade ao Tratamento Odontológico/epidemiologia , Feminino , Humanos , Estudos Longitudinais , Masculino , Prevalência , Fatores de Risco , Inquéritos e Questionários
14.
Artigo em Inglês | MEDLINE | ID: mdl-33744433

RESUMO

Rapid development of high-throughput technologies has permitted the identification of an increasing number of disease-associated genes (DAGs), which are important for understanding disease initiation and developing precision therapeutics. However, DAGs often contain large amounts of redundant or false positive information, leading to difficulties in quantifying and prioritizing potential relationships between these DAGs and human diseases. In this study, a network-oriented gene entropy approach (NOGEA) is proposed for accurately inferring master genes that contribute to specific diseases by quantitatively calculating their perturbation abilities on directed disease-specific gene networks. In addition, we confirmed that the master genes identified by NOGEA have a high reliability for predicting disease-specific initiation events and progression risk. Master genes may also be used to extract the underlying information of different diseases, thus revealing mechanisms of disease comorbidity. More importantly, approved therapeutic targets are topologically localized in a small neighborhood of master genes on the interactome network, which provides a new way for predicting drug-disease associations. Through this method, 11 old drugs were newly identified and predicted to be effective for treating pancreatic cancer and then validated by in vitro experiments. Collectively, the NOGEA was useful for identifying master genes that control disease initiation and co-occurrence, thus providing a valuable strategy for drug efficacy screening and repositioning. NOGEA codes are publicly available at https://github.com/guozihuaa/NOGEA.

15.
Acta Pharm Sin B ; 11(2): 442-455, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33643823

RESUMO

Although different types of drugs are available for postmenopausal osteoporosis, the limitations of the current therapies including drug resistances and adverse effects require identification of novel anti-osteoporosis agents. Here, we defined that norlichexanthone (NOR), a natural product, is a ligand of estrogen receptor-alpha (ERα) and revealed its therapeutic potential for postmenopausal osteoporosis. We used mammalian-one hybrid assay to screen for ERα modulators from crude extracts of several plant endophytes. As a result, NOR purified from the extract of endophyte ARL-13 was identified as a selective ERα modulator. NOR directly bound to ERα with an affinity in nanomolar range, revealing that it is a natural ligand of ERα. NOR induced osteoblast formation in MC3T3-E1 precursor cells. Conversely, NOR inhibited receptor activator of nuclear factor-kappa B ligand (RANKL)-induced osteoclast formation in both RAW264.7 macrophages and mouse primary monocytes. Mechanistically, NOR inhibited RANKL-induced association of ERα and TRAF6 to prevent ERα-mediated TRAF6 activation via Lys63-linked ubiquitination. Importantly, NOR exhibited potent anti-osteoporosis efficacy in an ovariectomized mouse model. Comparing to estrogen, NOR was of much less capability in stimulating endometrial hyperplasia and promoting mammalian cancer cell proliferation. Taken together, our study identified NOR as a natural and high affinity ligand of ERα with substantial anti-osteoporosis but less estrogenic activity.

16.
Orthop Surg ; 13(3): 791-798, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33709632

RESUMO

OBJECTIVE: To assess and compare the performance of fluorine-18-labeled fluorodeoxyglucose positron emission tomography (18 F-FDG-PET/ CT) and gallium-68-labeled tetraazacyclododecanetetraacetic acid-DPhe1-Tyr3-octreotate (68 Ga- DOTATATE) PET/CT in the targeted imaging of culprit tumors causing osteomalacia. METHODS: This was a clinical retrospective analysis. We analyzed 13 patients (five men, eight women; mean age, 49 years; range, 19-55 years) with suspicion of tumor-induced osteomalacia (TIO) between March 2017 and October 2019. All patients underwent two functional imaging methods to locate the culprittumors. Studies were performed on a PET/CT scanner. The injection doses of 18 F- FDG and 68 Ga-DOTATATE were 0.5mCi/kg and approximately 5.0mCi, respectively. In the two scans, the whole body was captured from head to toe 45 to 60 min after intravenous tracer injection. 68 Ga-DOTATATE PET/CT and 18 F-FDG PET/CT imaging results locate culprit tumors according to the following criteria: (i) abnormal foci uptake concentration was observed locally, and the uptake level was higher than the background level of the right lobe of the liver; (ii) combined CT showed or did not have obvious abnormal density changes; and (iii) non-specific ingestion lesions due to fracture, arthritis, necrosis of femoral head are excluded. Compared with the results of pathological examination and clinical follow-up, the sensitivity, specificity and accuracy of 68 Ga-DOTATATE PET/CT imaging and 18 F-FDG PET/CT imaging for TIO were analyzed. RESULTS: All patients had symptoms of osteomalacia and hypophosphatemia. The lag time (symptoms to PET diagnosis) ranged from 2 to 12 years. There were eight cases of TIO patients and five cases of non-TIO patients confirmed by surgery, pathology and follow-up. Among the eight TIO patients, there were six cases (75.0%) of PMTs, one case (12.5%) of giant cell tumor, one case (12.5%) of hemangiopericutoma. Most (n = 6, 75.0%) of the confirmed tumors in our patient population were in the lower extremities, followed by craniofacial regions (n = 1, 12.5%), and torso (n = 1, 12.5%), respectively. Among the five non-TIO patients, there were two cases of Fanconi syndrome, one case of rickets, and two cases of sporadic osteomalacia hypophosphorus. The culprit tumors could be located either in the bone (n = 5, 62.5%) or the soft tissue (n = 3, 37.5%). 18 F-FDG PET/CT was able to localize the tumor in six (6/13, 46.1%) patients. 68 Ga-DOTATATE PET/CT detected tumor in 8 (83.3%) of 13 patients. The sensitivity of 68 Ga-DOTATATE PET/CT imaging and 18 F-FDG PET/CT imaging in the evaluation of TIO in our patient population were 100% (8/8) vs 75% (6/8). The specificity of the two different methods was 80% (4/5). The overall accuracy was 92.3% (12/13) vs 76.9% (10/13). CONCLUSIONS: 68 Ga-DOTATATE PET/CT is very effective in assessing hypophosphatemia patients with TIO typical symptoms compared with 18 F-FDG. Therefore, in clinically suspected cases of hypophosphatemic osteomalacia, 68 Ga-DOTATATE PET/CT should be preferred as an imaging modality investigation to avoid delay in the treatment of this disease.


Assuntos
Osteomalacia/diagnóstico por imagem , Síndromes Paraneoplásicas/diagnóstico por imagem , Tomografia por Emissão de Pósitrons combinada à Tomografia Computadorizada , Adolescente , Adulto , Feminino , Radioisótopos de Flúor , Humanos , Masculino , Pessoa de Meia-Idade , Octreotida/análogos & derivados , Compostos Organometálicos , Compostos Radiofarmacêuticos , Estudos Retrospectivos , Adulto Jovem
17.
Chem Commun (Camb) ; 57(22): 2740-2743, 2021 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-33594998

RESUMO

Piezocatalysis is a promising approach for environmental pollutant removal. Monoclinic dibismuth tetraoxide (m-Bi2O4) was first applied to piezocatalyze organics under ultrasonic vibration. The built-in electric field with ultrasonic stress drives the separation of holes and electrons in m-Bi2O4. Its excellent piezocatalytic activity, reusability and chemical stability make m-Bi2O4 a new candidate of piezocatalysis.

18.
Fitoterapia ; 147: 104766, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33130231

RESUMO

Two new compounds, triacremoniate (1) and dietziamide C (2) along with known compounds ß-Adenosine (3) and acrepyrone A (4) were obtained from the mangrove-derived fungus Acremonium citrinum. MMF4. Their structures were unambiguously determined by extensive spectroscopic methods, including UV, IR, HRESIMS and NMR. Triacremoniate (1) can promote apoptosis of HeLa cells by increasing the PARP cleavage and the phosphorylation of JNK and p38.


Assuntos
Acremonium/química , Antineoplásicos/farmacologia , Produtos Biológicos/farmacologia , Antineoplásicos/isolamento & purificação , Produtos Biológicos/isolamento & purificação , China , Células HeLa , Humanos , Estrutura Molecular , Raízes de Plantas/microbiologia , Rhizophoraceae/microbiologia
19.
Brain Behav ; 10(11): e01827, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-32940411

RESUMO

OBJECTIVE: This study aimed to differentiate the variations in the clinical characteristics, MRI irregularity, and glucose metabolism on 18 F-FDG-PET for the differential diagnosis of Parkinson's Disease (PD), MSA with predominant Parkinsonism (MSA-P), and MSA with predominant cerebellar features (MSA-C). METHODS: Thirty PD patients, 22 MSA-P patients, and 28 MSA-C patients received an MRI and 20 PD patients, 11 MSA-P patients, and 13 MSA-C patients received 18 F-FDG-PET. RESULTS: Firstly, we found that the clinical data presented a tremor at rest, bradykinesia, and postural instability that was predominated in PD (100%), MSA-P (86.4%), and MSA-C (53.6%) patients, respectively. Then, we used MRI analyses and found that putamina atrophy and hyperintensive rim (T2 WI) were characteristic features in MSA-P and cerebellar atrophy, the "hot cross bun" sign and signal rise in the middle cerebellar peduncle were more obvious in MSA-C. To further explore the distinctions among the 3 diseases, we also used 18F-FDG-PET technology for our examination and found a decrease in glucose metabolism in the parietal area for Parkinson's Disease (PD), in the bilateral putamen for MSA-P, and in the bilateral cerebellum for MSA-C. CONCLUSION: This study identified the distinctive features of the clinic symptoms, MRI irregularity, and glucose metabolism on 18 F-FDG-PET, which provided a new basis for the differential diagnosis of Parkinson's Disease (PD), MSA with predominant Parkinsonism (MSA-P), and MSA with predominant cerebellar features (MSA-C).


Assuntos
Atrofia de Múltiplos Sistemas , Doença de Parkinson , Diagnóstico Diferencial , Fluordesoxiglucose F18 , Humanos , Imageamento por Ressonância Magnética , Atrofia de Múltiplos Sistemas/diagnóstico por imagem , Doença de Parkinson/diagnóstico por imagem
20.
ACS Appl Mater Interfaces ; 12(31): 34643-34657, 2020 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-32639712

RESUMO

Cochlear hair cells are critical for the conversion of acoustic into electrical signals and their dysfunction is a primary cause of acquired hearing impairments, which worsen with aging. Piezoelectric materials can reproduce the acoustic-electrical transduction properties of the cochlea and represent promising candidates for future cochlear prostheses. The majority of piezoelectric hearing devices so far developed are based on thin films, which have not managed to simultaneously provide the desired flexibility, high sensitivity, wide frequency selectivity, and biocompatibility. To overcome these issues, we hypothesized that fibrous membranes made up of polymeric piezoelectric biocompatible nanofibers could be employed to mimic the function of the basilar membrane, by selectively vibrating in response to different frequencies of sound and transmitting the resulting electrical impulses to the vestibulocochlear nerve. In this study, poly(vinylidene fluoride-trifluoroethylene) piezoelectric nanofiber-based acoustic circular sensors were designed and fabricated using the electrospinning technique. The performance of the sensors was investigated with particular focus on the identification of the resonance frequencies and acoustic-electrical conversion in fibrous membrane with different size and fiber orientation. The voltage output (1-17 mV) varied in the range of low resonance frequency (100-400 Hz) depending on the diameter of the macroscale sensors and alignment of the fibers. The devices developed can be regarded as a proof-of-concept demonstrating the possibility of using piezoelectric fibers to convert acoustic waves into electrical signals, through possible synergistic effects of piezoelectricity and triboelectricity. The study has paved the way for the development of self-powered nanofibrous implantable auditory sensors.


Assuntos
Acústica , Nanofibras/química , Polímeros/química , Eletricidade , Tamanho da Partícula , Propriedades de Superfície
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