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1.
Life Sci ; 286: 120048, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34655604

RESUMO

AIMS: Clinically, Cerebralcare Granule® (CG) has been widely utilized to treat various types of headache, chronic cerebral insufficiency and other diseases, and the effect is significant. Clinical studies have shown that CG can significantly relieve vascular dementia (VaD), however, the molecular mechanisms haven't been established. To clear the therapeutic mechanisms of CG against VaD, a hypothesis was proposed that CG could treat neurovascular injury by inhibiting the production of lipocalin-2 (LCN 2). MAIN METHODS: 90 dementia rats were selected by water maze test and randomly divided into 6 groups, including nimodipine (NM), CG L (low dose) (0.314 g kg-1), CG H (high dose) (0.628 g kg-1), and combined group (CG + NM). And in vitro neuronal cell OGD modeling to evaluate the effect of CG on JAK2/STAT3. KEY FINDINGS: CG could significantly shorten the escape latency of two-vessel occlusion (2-VO) rats, increase their exploratory behavior, alleviate the symptoms of VaD and improve the ultrastructural pathological damage of neurovascular unit and accelerate the recovery of cerebral blood perfusion. CG combined with NM is better than NM alone. It was further showed that CG could inhibit the pathogenicity of LCN 2 through JAK2/STAT3 pathway and suppress the production of inflammatory cytokines. It plays a role in the protection of cerebral microvasculature and BBB in 2-VO rats. SIGNIFICANCE: Taken together, there data has supported notion that CG can protect the integrity of cerebral blood vessels and BBB and improve cognitive impairment through mainly inhibiting LCN 2, which provides scientific evidence for clinical application.


Assuntos
Disfunção Cognitiva/tratamento farmacológico , Medicamentos de Ervas Chinesas/metabolismo , Lipocalina-2/metabolismo , Animais , Artérias Carótidas/efeitos dos fármacos , China , Disfunção Cognitiva/fisiopatologia , Demência Vascular/prevenção & controle , Modelos Animais de Doenças , Medicamentos de Ervas Chinesas/farmacologia , Lipocalina-2/fisiologia , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Fármacos Neuroprotetores/farmacologia , Nimodipina/metabolismo , Nimodipina/farmacologia , Ratos , Ratos Sprague-Dawley
2.
Zhongguo Zhong Yao Za Zhi ; 46(11): 2737-2745, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34296570

RESUMO

Drynariae Rhizoma is warm in nature and bitter in taste, mainly acting on liver and kidney systems. It is a common Chinese herbal medicine for the treatment of fracture and bone injury. The chemical compositions of Drynariae Rhizoma mainly include flavonoids, triterpenoids, phenylpropanoids and lignans. At present, modern pharmacological and clinical studies have shown that Drynariae Rhizoma has the effects of anti osteoporosis, promoting fracture healing, kidney protection, anti-inflammatory, promoting tooth growth, preventing and treating aminoglycoside ototoxicity and lowering blood lipid. In addition, the toxicity evaluation experiment of Drynariae Rhizoma has also shown that it has no obvious toxic and side effects. Naringin is a kind of dihydroflavone in Drynariae Rhizoma. Many studies have shown that naringin and other total flavonoids play an important role in anti-osteoporosis, promoting fracture healing, anti-inflammation, promoting tooth growth and lowering blood lipid. In this study, the research progresses on chemical consti-tuents and pharmacological activities of Drynariae Rhizoma in recent years were reviewed, and some mechanisms of action were summarized, to provide references for the further research and development of Drynariae Rhizoma.


Assuntos
Medicamentos de Ervas Chinesas , Osteoporose , Polypodiaceae , Medicamentos de Ervas Chinesas/farmacologia , Flavonoides , Humanos , Osteoporose/tratamento farmacológico , Rizoma
3.
Chin J Nat Med ; 18(6): 417-424, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32503733

RESUMO

In recent years, biosynthesis of triterpenoid saponins in medicinal plants has been widely studied because of their active ingredients with diverse pharmacological activities. Various oxidosqualene cyclases, cytochrome P450 monooxygenases, uridine diphosphate glucuronosyltransferases, and transcription factors related to triterpenoid saponins biosynthesis have been explored and identified. In the biosynthesis of triterpenoid saponins, the progress of gene mining by omics-based sequencing, gene screening, gene function verification, catalyzing mechanism of key enzymes and gene regulation are summarized and discussed. By the progress of the biosynthesis pathway of triterpenoid saponins, the large-scale production of some triterpenoid saponins and aglycones has been achieved through plant tissue culture, transgenic plants and engineered yeast cells. However, the complex biosynthetic pathway and structural diversity limit the biosynthesis of triterpenoid saponins in different system. Special focus can further be placed on the systematic botany information of medicinal plants obtained from omics large dataset, and triterpenoid saponins produced by synthetic biology strategies, gene mutations and gene editing technology.


Assuntos
Plantas Medicinais/química , Plantas Medicinais/genética , Saponinas/biossíntese , Triterpenos/química , Vias Biossintéticas , Sistema Enzimático do Citocromo P-450/fisiologia , Regulação da Expressão Gênica de Plantas , Glucuronosiltransferase/fisiologia , Estrutura Molecular , Plantas Geneticamente Modificadas , Fatores de Transcrição , Difosfato de Uridina/fisiologia
5.
J Agric Food Chem ; 66(36): 9446-9455, 2018 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-30095259

RESUMO

Ginsenosides attract great attention for their bioactivities. However, their contents are low, and many UDP-glycosyltransferases (UGTs) that play crucial roles in the ginsenoside biosynthesis pathways have not been identified, which hinders the biosynthesis of ginsenosides. In this study, we reported that one UDP-glycosyltransferase, UGTPg71A29, from Panax ginseng could glycosylate C20-OH of Rh1 and transfer a glucose moiety to Rd, producing ginsenosides Rg1 and Rb1, respectively. Ectopic expression of UGTPg71A29 in Saccharomyces cerevisiae stably generated Rg1 and Rb1 under its corresponding substrate. Overexpression of UGTPg71A29 in transgenic cells of P. ginseng could significantly enhance the accumulation of Rg1 and Rb1, with their contents of 3.2- and 3.5-fold higher than those in the control, respectively. Homology modeling, molecular dynamics, and mutational analysis revealed the key catalytic site, Gln283, which provided insights into the catalytic mechanism of UGTPg71A29. These results not only provide an efficient enzymatic tool for the synthesis of glycosides but also help achieve large-scale industrial production of glycosides.


Assuntos
Ginsenosídeos/biossíntese , Glicosiltransferases/química , Glicosiltransferases/metabolismo , Panax/enzimologia , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Motivos de Aminoácidos , Vias Biossintéticas , Catálise , Domínio Catalítico , Glicosiltransferases/genética , Simulação de Dinâmica Molecular , Panax/química , Panax/genética , Proteínas de Plantas/genética , Difosfato de Uridina/metabolismo
6.
Zhongguo Zhong Yao Za Zhi ; 43(1): 168-173, 2018 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-29552828

RESUMO

To compare the therapeutic effects of different treatment methods on the nude mice bearing colon cancer HT29 cells. BalB/C nude mice colon cancer stem cell models were established and randomly divided into the following four groups, with 8 nude mice in each group: blank control group, DC-CIK group, Huaier group, and Huaier combined with DC-CIK group (combined treatment group). The mice in DC-CIK group and combined treatment group received 1×106 DC-CIK cells treatment by tail vein injectionafter the tumor stem cells were inoculated for 4 days,2 times a week for three weeks. The mice in Huaier group and combined treatment group received intragastric administration at the dose of 20 g/60 kg body weight, 0.2 mL/time, once a day for a total of three weeks. The mice in control group received equal volume of normal saline. Tumor size and body weight of nude mice were measured every 2 days during treatment for three weeks in each group. After the treatment, the nude mice were sacrificed to measure the tumor weight and the tumor inhibition rate was calculated. The RT-PCR method was used to detect the expression levels of the key genes in the signal pathway. After the end of the treatment, the quality of the tumor in the Huaier group, DC-CIK group and combined treatment group was significantly lower than that in the control group; the quality in combined treatment group was significantly lower than that in Huaier group and DC-CIK group.Among them, the tumor inhibition rate reached 46.77% in the combined treatment group. In respect of changes in expression levels of key genes in the signaling pathway, the mRNA expression levels of key genes PI3KR1 and Akt in PI3K/Akt pathway, key genes Wnt1 and CTTNB1 in Wnt/ß-catenin pathway, and key genes Notch1, Notch2, Notch3 in Notch pathway in the combined treatment group were lower than those in DC-CIK group and Huaier group. The Huaier combined with DC-CIK group showed best therapeutic effect among different treatment methods for HT29 stemcell colon tumors in nude mice, providing a new idea for clinical treatment of colon cancer.


Assuntos
Neoplasias do Colo/tratamento farmacológico , Misturas Complexas/farmacologia , Células-Tronco Neoplásicas/efeitos dos fármacos , Animais , Linhagem Celular Tumoral , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Transdução de Sinais/efeitos dos fármacos , Trametes
7.
J Agric Food Chem ; 66(11): 2917-2924, 2018 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-29504397

RESUMO

Formononetin and its glycoside ononin are bioactive isoflavones widely present in legumes. The present study investigated the pharmacokinetics, bioavailability, and in vitro absorption of formononetin and ononin. After an oral administration to rats, formononetin showed a higher systemic exposure over ononin. The oral bioavailability of formononetin and ononin were 21.8% and 7.3%, respectively. Ononin was more bioavailable than perceived, and its bioavailability reached 21.7% when its metabolite formononetin was taken into account. Both formononetin and ononin exhibited better absorption in large intestine segments than that in small intestine segments. Formononetin displayed a better permeability in all intestinal segments over ononin. Transport of formononetin across Caco-2 cell monolayer was mainly through passive diffusion, while ononin was actively pumped out by MRP2 but not P-gp. The results provide evidence for better understanding of the pharmacological actions of formononetin and ononin, which advocates more in vivo evaluations or human trials.


Assuntos
Glucosídeos/farmacocinética , Isoflavonas/farmacocinética , Extratos Vegetais/farmacocinética , Animais , Disponibilidade Biológica , Transporte Biológico , Células CACO-2 , Humanos , Absorção Intestinal , Mucosa Intestinal/metabolismo , Intestinos/química , Masculino , Modelos Biológicos , Permeabilidade , Ratos , Ratos Sprague-Dawley
8.
Zhongguo Zhong Yao Za Zhi ; 42(13): 2453-2459, 2017 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-28840683

RESUMO

The study is aimed to characterize the tissue expression of 10 key ginsenoside biosynthetic genes using bioinformatics method and real-time quantitative PCR. Heatmap and cluster analysis of 10 ginsenoside biosynthetic genes were performed in four-year-old Jilin ginseng. Using real-time quantitative PCR, the expression correlation of 10 key genes involved in ginsenoside biosynthesis was analyzed in different organs of four-year-old Jilin ginseng including, tissue culture seedling and adventitious root. Pearson correlation was used to analyze the relation between those 10 key genes involved in ginsenoside biosynthesis. The results showed that ß-AS and CYP716A52v2 were expressed highly in root of Jilin ginseng and ginseng culture seedling, which was consistent with Ro distribution. In addition, CYP716A53v2 and CYP716A47 which involved in dammarane type ginsenoside biosynthesis were positively correlated, which revealed that the difference of ginsenoside distribution was caused by transport system.


Assuntos
Expressão Gênica , Ginsenosídeos/biossíntese , Panax/genética , Raízes de Plantas/genética , Perfilação da Expressão Gênica , Panax/metabolismo , Raízes de Plantas/metabolismo
9.
Zhongguo Zhong Yao Za Zhi ; 42(12): 2236-2246, 2017 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-28822175

RESUMO

Plant tissue culture technology has been widely used in the field of traditional Chinese medicine(TCM) resources with its unique advantages, playing an important role in the protection of TCM resources. In this review, some applications of plant tissue culture were summarized, including production of active compounds by using plant tissue culture, genetic diversity analysis, Dao-di herbs, elicitor application, biosynthesis and transgenic plants. Through the above researches will promote the further development of plant tissue culture technology, making it play a greater role in the field of TCM resources.


Assuntos
Medicamentos de Ervas Chinesas , Plantas Medicinais/crescimento & desenvolvimento , Técnicas de Cultura de Tecidos , Medicina Tradicional Chinesa , Plantas Geneticamente Modificadas/crescimento & desenvolvimento
10.
BMC Cardiovasc Disord ; 17(1): 106, 2017 05 02.
Artigo em Inglês | MEDLINE | ID: mdl-28464794

RESUMO

BACKGROUND: With the marked increase in the prevalence of diabetes mellitus, it was the purpose of our study to assess a potential association of time-cumulated exposure to systolic (CumSBP) and of diastolic blood pressure (CumDBP) with onset of impaired glucose tolerance and diabetes mellitus. METHODS: The prospective investigation included participants of the longitudinal Kailuan Study with three baseline examinations in 2006-2007, 2008-2009 and 2010-2011, re-examination in 2012-2013, and no diabetes mellitus at baseline. Cumulative blood pressure (BP) was calculated as cumBP = [(BP1 + BP2)/2 × time1-2] + [(BP2 + BP3)/2 × time2-3]. Based on cumSBP, the study population was stratified into four groups (cumSBP < 480mmHgxyear;n = 15,339; 480mmHgxyear ≤ cumSBP < 520mmHgxyear;n = 7214; 520mmHgxyears ≤ cumSBP < 560mmHgxyears;n = 5675; and cumSBP ≥ 560mmHgxyears;n = 10,576). RESULTS: After adjusting for demographic, anthropomorphic, biochemical, socioeconomic and lifestyle parameters and as compared with the first group, the second, third and fourth group showed a significantly higher incidence of diabetes (P-trend < 0.001;hazard ratio (HR);95% confidence interval (CI):1.28(1.08,1.51),1.54(1.29,1.84), and 2.33(1.98,2.73), respectively), higher incidence of impairment of glucose tolerance (P-trend < 0.001;HR;95% CI1.17(1.02,1.33), 1.43(1.25,1.64), and 2.09(1.85,2.37), respectively), and higher incidence of diabetes developing out of an impairment of glucose tolerance (P-trend < 0.001;HR;95% CI:1.22(0.97,1.54),1.47(1.16,1.86), and 2.01(1.62,2.50), respectively). An increase in cumSBP by 10 mmHg/year or an increase in cumDBP by 5 mmHg/year was associated with a hazard ratio of incident diabetes of 1.04 (95% CI:1.03,1.04) and 1.02(1.02,1.03), respectively, with a hazard ratio of incident impairment of glucose tolerance of 1.04(95% CI:1.03,1.04) and 1.03(95% CI:1.02,1.03), respectively, and with a hazard ratio of incident diabetes developing from impairment of glucose tolerance of 1.04(95% CI:1.03,1.04) and 1.03(95% CI:1.02,1.03), respectively. CONCLUSIONS: Time-cumulated exposure to elevated blood pressure was significantly associated with an elevated incidence of impaired glucose tolerance and diabetes.


Assuntos
Glicemia/metabolismo , Pressão Sanguínea , Diabetes Mellitus/epidemiologia , Intolerância à Glucose/epidemiologia , Hipertensão/epidemiologia , Adulto , Biomarcadores/sangue , China/epidemiologia , Diabetes Mellitus/sangue , Diabetes Mellitus/diagnóstico , Feminino , Intolerância à Glucose/sangue , Intolerância à Glucose/diagnóstico , Humanos , Hipertensão/diagnóstico , Hipertensão/fisiopatologia , Incidência , Masculino , Pessoa de Meia-Idade , Modelos de Riscos Proporcionais , Estudos Prospectivos , Fatores de Risco , Fatores de Tempo
11.
Oncotarget ; 8(28): 46081-46089, 2017 Jul 11.
Artigo em Inglês | MEDLINE | ID: mdl-28537906

RESUMO

This study aims to compare the efficacy of different treatments for nude mice transplanted with HT-29 colon carcinoma cell line. BalB/C nude mice were transplanted with HT-29 colon carcinoma cell line and randomly divided into four groups, with 5 mice in each group: blank control group, DC-CIK group, Huaier Granule group, and Huaier Granule group combined with DC-CIK group (combined treatment group). For DC-CIK group and combined treatment group, 1×106 DC-CIK cells were injected via the tail vein 4 days after transplantation. The injection was performed twice weekly for a total of 2 weeks. For Huaier Granule group and combined treatment group, Huaier Granule was administered at the dose of 20 g/60 g, by dissolving 20 g of Huaier granules in 600 ml of pure water. Intragastric administration of 0.2 ml of granules was performed once daily for 3 weeks. For the blank control group, equal volume of normal saline was given. Tumor size and body weight of nude mice were measured every 2 days during the 3-week treatment. The mice were sacrificed at the end of treatment to harvest tumors. Key genes of the signaling pathway were detected by RT-PCR. At the end of treatment, mice in combined treatment group, DC-CIK group and Huaier Granule group remained stable emotionally with normal mobility and water and food intake. However, in the blank control group, the mobility was restricted starting from the third week and the mice were on the verge of dying. The expression of PI3KR1, Akt, Wnt1, CTTNB1, Notch1, Notch2 and Notch3 genes were all downregulated significantly in the combined treatment group compared with DC-CIK group and Huaier Granule group (P<0.05). Therefore, the combined treatment of Huaier Granule combined with DC-CIK achieved the best effect in nude mice transplanted with HT-29 colon carcinoma cell line.


Assuntos
Antineoplásicos/uso terapêutico , Vacinas Anticâncer/imunologia , Neoplasias do Colo/terapia , Misturas Complexas/uso terapêutico , Células Matadoras Induzidas por Citocinas/imunologia , Células Dendríticas/imunologia , Imunoterapia Adotiva/métodos , Neoplasias Experimentais/terapia , Animais , Apoptose , Neoplasias do Colo/imunologia , Terapia Combinada , Células Matadoras Induzidas por Citocinas/transplante , Células Dendríticas/transplante , Células HT29 , Humanos , Masculino , Medicina Tradicional Chinesa , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Neoplasias Experimentais/imunologia , Transdução de Sinais , Trametes , Carga Tumoral
12.
Zhongguo Zhong Yao Za Zhi ; 42(22): 4426-4430, 2017 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-29318846

RESUMO

The study aims at investigating the ecological environment Adiantum reniforme var. sinense of in Three Gorges Reservoir region, and providing a reference basis for the protection of resources and artificial cultivation of A. reniforme var. sinense. By using the method of investigation, field survey and experimental analysis, the vegetation, natural geographical environment, climate, soil nutrients of A. reniforme var. sinense were studied and analyzed. The survey found that A. reniforme var. sinense distribution area reduced fast in Three Gorges region, a lot of distribution has diminished and vanished due to excessive digging, currently only in 3 towns of Wanzhou there exist 4 wild distribution areas. The growth of A. reniforme var. sinense needs an environment with low altitude, steep slope and thin soil, northeast slope, canopy height and warm and humid climate characteristics, and the soil in distribution has the characteristics of high organic matter, available nitrogen, available potassium, and low available phosphorus content.


Assuntos
Adiantum/crescimento & desenvolvimento , Dispersão Vegetal , China , Clima , Nitrogênio , Fósforo , Plantas Medicinais/crescimento & desenvolvimento , Potássio , Solo/química
13.
Zhongguo Zhong Yao Za Zhi ; 41(9): 1732-1738, 2016 May.
Artigo em Chinês | MEDLINE | ID: mdl-28891626

RESUMO

To conduct multiple-reaction monitoring(MRM) quantitative analysis with high-performance liquid chromatography coupled with mass spectrometry method, establish the quantification method of magnolol and honokiol in blood sample under negative ion mode with ibuprofen as internal standard, investigate the pharmacokinetic process of lignans constituents after oral administration of Weichang'an pill(WCA) at different doses, and provide theoretical basis to further reveal the material basis of WCA's anti-diarrhea effect. In the plasma samples, the linear relationship was good over the concentration range of 5.25 to 1 344.00 µg•L ⁻¹ for magnolol and 10.08 to 2 580.00 µg•L ⁻¹ for honokiol. The results of precision, stability, and extraction recovery tests showed that the determination method of plasma concentration for such compositions was stable and reliable. Dose-dependence was shown for magnolol and honokiol in the plasma concentration-time profile. The results indicated that the time to reach the maximum plasma concentration(Tmax) for lignanoids was 0.55-1.42 h, when the maximum plasma concentration(Cmax) could reach 996.36-2 330.96,189.87-1 469.43 µg•L ⁻¹ respectively for magnolol and honokiol. The lignanoids could be absorbed rapidly in the blood after oral administration of WAC pills, providing experimental basis to prove rapid and long-acting anti-diarrhea effect of WAC pills after oral administration.


Assuntos
Compostos de Bifenilo/farmacocinética , Medicamentos de Ervas Chinesas/farmacocinética , Lignanas/farmacocinética , Animais
14.
Zhongguo Ying Yong Sheng Li Xue Za Zhi ; 32(3): 230-233, 2016 Mar 08.
Artigo em Chinês | MEDLINE | ID: mdl-29931882

RESUMO

OBJECTIVE: To discuss the modulating effects of shenmai (Traditional Chinese Medicine) injection on blood lipid in hyperlipidemia rats through observing the serum lipid level and a series of related biochemical indexes in hyperlipidemia rat model. METHODS: A total of 30 male SD rats of SPF grade were purchased and fed with basic feed for 1 week to adapt the environment. Then the rats were randomly divided into the following groups(n=10):control group, model control group, shenmai injection group. Control group was fed basal diet; the latter two groups were fed high fat diet,the body weight of all the animals was measured each week. For shenmai injection group, the rats were fed with shenmai injection (10 ml/kg) twice a day for 45 consecutive days through oral administration. The effects of shenmai injection on body weight, serum triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), super oxide dismutase (SOD), glutathione peroxidase(GSH-Px), malondialdehyde (MDA), the activities of lipoprotein lipase(LPL) and hepatic lipase (HL) in hyperlipidemia rats were detected. RESULTS: Body weight, serum of TC, TG, LDL-C, HDL-C, and MDA levels in model control group were significantly higher(P<0.01), while serum of HDL-C, SOD, GSH-Px, LPL, and HL levels were significantly lower than those of control group(P<0.01). Body weight, serum of TC, TG, LDL-C, HDL-C, and MDA levels in shenmai injection group were significantly lower(P<0.01), while serum of HDL-C, SOD, GSH-Px, LPL, and HL levels were significantly higher than those of model group(P<0.05, P<0.01). CONCLUSIONS: Shenmai injection has a significant effect of modulating blood lipid and antioxidant function on hyperlipidemia rats.


Assuntos
Colesterol/sangue , Medicamentos de Ervas Chinesas/farmacologia , Hiperlipidemias/tratamento farmacológico , Triglicerídeos/sangue , Animais , Antioxidantes/metabolismo , Combinação de Medicamentos , Masculino , Ratos , Ratos Sprague-Dawley
15.
Zhongguo Zhong Yao Za Zhi ; 40(13): 2589-93, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-26697683

RESUMO

Cassiae Semen is a common traditional Chinese medicine, and contents of anthraquinones of Cassiae Semen different significantly from area to area. According to Chinese Pharmacopoeia (2010 edition), only contents of aurantio obtusin and chrysophanol were used to evaluate the quality of Cassiae Semen, another data could be added later. Ten batches of Cassiae Semen from different areas were determined, and total anthraquinones, total free anthraquinones and total combined anthraquinones contents were assessed by ultraviolet visible spectrophotometer, contents of aurantio obtusin, rhein, aloe emodin, emodin, chrysophanol and physcion were determined by HPLC. After that, principal components analysis was used to evaluate these data determined previous by dimension reduction analysis. At last, the result suggests that three main components were found out, it shows that content of aloe emodin could be used to evaluate the quality of Cassiae Semen as well as contents of aurantio obtusin and chrysophanol. And Cassiae Semen from Hebei province posseses higher quality than Cassiae Semen from other different areas. All these results can provide a good reference for quality evaluating of Cassiae Semen medicinal materials at a certain extent.


Assuntos
Antraquinonas/análise , Cassia/química , Cromatografia Líquida de Alta Pressão , Análise de Componente Principal
16.
Zhongguo Zhong Yao Za Zhi ; 40(11): 2112-6, 2015 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-26552165

RESUMO

Astragalus polysaccharide has been widely used in food and medicinal industry owing to its health-promoting properties. In order to characterize better the relationship among molecular weight, structure-activity and activities, a simple method was used different concentration of ethanol including 30% (PW30), 50% (PW50), 70% (PW70), 75% (PW75), 80% (PW80) and 90% (PW90) to precipitate Astragalus polysaccharides into different molecular weight. As a result, PW90 showed smooth surface and the strongest antioxidant activity among these six fractions (P < 0.05). In conclusion, graded ethanol precipitation was a simple method to separate Astragalus polysaccharides into different molecular weight with different antioxidant activity fractions.


Assuntos
Antioxidantes/farmacologia , Astrágalo (Planta)/química , Polissacarídeos/química , Precipitação Química , Etanol/química , Polissacarídeos/farmacologia
17.
Zhongguo Zhong Yao Za Zhi ; 40(6): 1173-8, 2015 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-26226766

RESUMO

A HPLC-MS/MS multiple-reaction monitoring (MRM) quantitative analysis was made to establish a determination method for drug concentrations of costunolide (Co) and dehydrocostuslactone (De) in blood samples in the positive ion mode, with diazepam as the internal standard substance, in order to study the pharmacokinetic process of sesquiterpene lactones costunolide and dehydrocostuslactone after the oral administration of Weichang'an pills, and provide an theoretical basis for further studies on the substance basis for the anti-diarrhea effect of Weichang'an pills. In the blood samples, Co and De showed a good linearity within concentration ranges 0.700 0-769.7, 2.510-956.0 µg x L(-1), respectively. The results of precision, stability and recovery experiences proved the stability and reliability of the plasma concentration determination method. After the oral administration, the concentrations of Co and De in plasma increased with the increase in dose, with T(max) between 10.65-12.98 h, indicating a long time to reach peak plasma concentrations; C(max) of costunolide and dehydrocostuslactone ranged between 3.750-5.450,15.34-44.52 µg x L(-1), respectively. The in vivo adsorption of Co and De conformed to the one-compartment model, with a longer time to attain the peak plasma concentrations. These results provided an experimental basis for revealing the active substance basis and clinical medication of Weichang'an pills.


Assuntos
Medicamentos de Ervas Chinesas/farmacocinética , Lactonas/farmacocinética , Sesquiterpenos/farmacocinética , Administração Oral , Animais , Medicamentos de Ervas Chinesas/administração & dosagem , Medicamentos de Ervas Chinesas/química , Lactonas/administração & dosagem , Lactonas/sangue , Masculino , Ratos , Ratos Wistar , Sesquiterpenos/administração & dosagem , Sesquiterpenos/sangue , Comprimidos/administração & dosagem , Comprimidos/química , Comprimidos/farmacocinética
18.
J Sci Food Agric ; 95(4): 836-42, 2015 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-25355080

RESUMO

BACKGROUND: Dioscorea resources with vital medicinal and functional values are abundant in south-west regions of China, especially in Sichuan Province. However, the resource in this region has received less attention compared with that of the north. D. zingiberensis, D. collettii, D. kamoonensis cv. Emei and Jinfo, and D. melanophyma from Sichuan Province and Chongqing City were studied with regard to the most abundant carbohydrate (starch) to search for new medicinal and food resources. RESULTS: The starches were small round granules or small oval granules and large elongated granules, except D. zingiberensis starch granules, which were disc-like in shape. D. zingiberensis and D. collettii starches showed higher values in total starch content, water-binding capacity and infrared ratio of absorbance bands at 1047/1035 and 1047/1022 cm⁻¹. Differential scanning calorimetry analysis demonstrated a higher gelatinisation temperature required more energy during the gelatinisation process. D. zingiberensis and D. collettii starches showed higher resistant starch content of 724.0 and 693.2 g kg⁻¹, respectively, with lower hydrolysis index and estimation of glycaemic index. All the starches exhibited an A-type pattern except D. melanophyma starch, which showed a C-type pattern evaluated by X-ray diffraction. CONCLUSION: These results showed that the starches with their low hydrolysis index values possessed potential values as healthy food.


Assuntos
Digestão , Dioscorea/química , Rizoma/química , Amido/metabolismo , Animais , Fenômenos Químicos , China , Produtos Agrícolas/química , Produtos Agrícolas/crescimento & desenvolvimento , Produtos Agrícolas/metabolismo , Dioscorea/crescimento & desenvolvimento , Dioscorea/metabolismo , Géis , Glucana 1,4-alfa-Glucosidase/metabolismo , Índice Glicêmico , Humanos , Hidrólise , alfa-Amilases Pancreáticas/metabolismo , Tamanho da Partícula , Rizoma/crescimento & desenvolvimento , Rizoma/metabolismo , Solubilidade , Especificidade da Espécie , Amido/análise , Amido/biossíntese , Amido/isolamento & purificação , Sus scrofa , Temperatura de Transição , Água/análise
19.
Zhongguo Zhong Yao Za Zhi ; 40(17): 3470-9, 2015 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-26978991

RESUMO

Based on the results of the morphologic studies on genus Dioscorea, the paper summarized the entire chemical constituent that isolated from this genus and analyzed it with the methods of chemotaxonomy. The rules of the chemical constituent and pharmacodynamic effects were analyzed. Seventeen species which belong to Sect. Stenophora Uline of Dioscorea contain steroidal sapogenin. Other species with different main components such as polysaccharide and tannin have have different effects. This chemotaxonomic view point will conduce to establish a phylogeny of the genus Dioscorea.


Assuntos
Dioscorea/química , Dioscorea/genética , Animais , China , Dioscorea/classificação , Dioscorea/crescimento & desenvolvimento , Humanos , Filogenia , Extratos Vegetais/química , Extratos Vegetais/farmacologia
20.
Zhongguo Zhong Yao Za Zhi ; 39(15): 2851-8, 2014 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-25423821

RESUMO

Rheumatoid arthritis (RA) is a kind of chronic, progressive, multiple, invasive autoimmune disease with two chief cclinical manifestations arthrosynovitis and ex-arthrosis, easy to occur in middle-aged women, also occur in children and the elderly, is characterized by progressive and break out repeatedly. RA pathogenesis is complex, there is no special treatment, used in treatment of R drug varied, new drugs and new therapies also emerge in endlessly, main including non-steroidal anti-inflammatory drugs (NSAIDs), slow action anti-rheumatism medicine (SAARDs), glucocorticoids (GCs), biological agent, traditional Chinese medicine and traditional Chinese medicine preparations, domestic market for rheumatoid main drug treatment are NSAIDs, SAARDs, GCs, traditional Chinese medicine and traditional Chinese medicine preparations. Traditional Chinese medicine and traditional Chinese medi- cine preparations for the treatment of RA have its unique advantages, show the characteristics of overall adjustment, multi-level and multiple targets, and also can alleviate and against side effects of western medicine. In recent years, more and more get people's atten- tion. This paper reviewed the research progress and treatment features of commonly used therapeutic agents for the treatment of RA in recent years, which provides reference and basis for future medicine anti-RA.


Assuntos
Artrite Reumatoide/tratamento farmacológico , Descoberta de Drogas/métodos , Animais , Fatores Biológicos/efeitos adversos , Fatores Biológicos/uso terapêutico , Glucocorticoides/efeitos adversos , Glucocorticoides/uso terapêutico , Humanos , Medicina Tradicional Chinesa/efeitos adversos
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