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1.
Zhongguo Zhong Yao Za Zhi ; 46(12): 3058-3065, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34467696

RESUMO

In this study, the compound search was completed through SciFinder and CNKI databases, and the drug-like properties were screened in FAFdrugs4 and SEA Search Server databases. In addition, based on the target sets related to acute myocardial ischemia(AMI) searched in disease target databases such as OMIM database, GeneCards database and DrugBank, a network diagram of chemical component-target-pathway-disease was established via Cytoscape to predict the potential active components of Corydalis Herba, a traditional Tibetan herbal medicine which derived from the aerial parts of Corydalis hendersonii and C. mucronifera against AMI. A protein-protein interaction(PPI) network was constructed through the STRING database and the core targets in the network were predicted. And the enrichment analyses of core targets were completed by DAVID database and R software. Furthermore, a molecular docking method was used to verify the binding of the components with core targets using softwares such as Autodock Vina. The present results showed that there were 60 compounds related to AMI in Corydalis Herba, involving 73 potential targets. The GO functional enrichment analysis obtained 282 biological processes(BP), 49 cell components(CC) and 78 molecular functions(MF). KEGG was enriched into 85 pathways, including alcoholism pathway, endocrine resistance pathway, calcium signaling pathway, cAMP signaling pathway, vascular endothelial growth factor signaling pathway and adrenergic signaling transduction pathway of myocardial cells. The results of network topology analysis showed that the key components of anti-AMI of Corydalis Herba might be tetrahydropalmatine, etrahydrocolumbamine, N-trans-feruloyloctopamine, N-cis-p-coumaroyloctopamine, N-trans-p-coumaroylnoradrenline and N-trans-p-coumaroyloctopamine, and their core targets might be CDH23, SCN4 B and NFASC. The results of molecular docking showed that the key components of Corydalis Herba had stable binding activity with the core targets. This study provides reference for further elucidation of the pharmacological effects of Corydalis Herba against AMI, subsequent clinical application, and development.


Assuntos
Corydalis , Medicamentos de Ervas Chinesas , Isquemia Miocárdica , Medicamentos de Ervas Chinesas/farmacologia , Medicina Tradicional Tibetana , Simulação de Acoplamento Molecular , Isquemia Miocárdica/tratamento farmacológico , Fator A de Crescimento do Endotélio Vascular
2.
Zhongguo Zhong Yao Za Zhi ; 46(13): 3257-3269, 2021 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-34396745

RESUMO

Cardiovascular diseases seriously endanger human health and life. The accompanying myocardial injury has been a focus of attention in society. Chinese medicine,serving as a natural and precious reservoir for the research and development of new drugs,is advantageous in resisting myocardial injury due to its multi-component,multi-pathway,and multi-target characteristics. In recent years,with the extensive application of culture method for isolated cardiomyocytes,a cost-effective,controllable in vitro model of cardiomyocyte injury with uniform samples is becoming a key tool for mechanism research on cardiomyocyte injury and drug development.A good in vitro model can reduce experimental and manpower cost,and also accurately stimulate clinical changes to reveal the mechanism. Therefore,the selection and establishment of in vitro model are crucial for the in-depth research. This study summarized the modeling principles,evaluation indicators,and application of more than ten models reflecting different clinical conditions,such as injuries induced by hypoxia-reoxygenation,hypertrophy,oxidative stress,inflammation,internal environmental disturbance,and toxicity. Furthermore,we analyzed advantages and technical difficulties,aiming to provide a reference for in-depth research on myocardial injury mechanism and drug development.


Assuntos
Apoptose , Miócitos Cardíacos , Hipóxia Celular , Humanos , Miocárdio , Estresse Oxidativo
3.
Chin J Dent Res ; 24(2): 105-112, 2021 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-34219443

RESUMO

adolescents aged 12 to 15 years based on the 4th National Oral Health Survey and to explore its associated factors. METHODS: Students aged 12 to 15 years were recruited using to a multistage stratified random sampling method. All the subjects received oral examination and completed a questionnaire. Information relating to OHRQoL was collected through a Mandarin version of the child oral impacts on daily performances (Child-OIDP) questionnaire. The relationship between the Child-OIDP scores and independent variables was assessed using a Mann-Whitney U-test, Kruskal-Wallis test and a multivariate Poisson regression. RESULTS: A total of 89,582 subjects were included in the analysis, of whom 76.6% reported oral impacts in the last 6 months. Eating was the most affected daily performance. The results of the regression analysis showed that factors associated with adolescents' OHRQoL included sex, location of residence, region, only child status, parents' level of education, frequency of sugar consumption, self-perception of general/oral health, dental appointments in the past 12 months, oral health knowledge status, age, decayed, missing and filled teeth (DMFT) index and number of teeth with gingival bleeding. CONCLUSION: Oral impacts were common among Chinese adolescents, although most were not so severe. Eating was the most commonly affected performance. Sex, location of residence, region, only child status, parents' level of education, frequency of sugar consumption, selfperception of general/oral health status, dental appointments in the previous 12 months, oral health knowledge status, age, DMFT index and number of teeth with gingival bleeding were found to be associated with OHRQoL.


Assuntos
Cárie Dentária , Qualidade de Vida , Adolescente , China , Estudos Transversais , Inquéritos de Saúde Bucal , Humanos , Saúde Bucal , Inquéritos e Questionários
4.
Sci Total Environ ; 785: 147320, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-33957589

RESUMO

Globally, the proliferation of shrubs within grasslands stimulates soil phosphorus (P) cycling and increases topsoil P storage beneath their canopies. However, little is known regarding the impact of shrub encroachment on subsoil P storage, and whether shrubs mediate changes in soil stoichiometry, like increasing P cycling. In grazed meadows on the Tibetan Plateau, soil and roots were sampled to 1 m depth in shrubby Hippophae rhamnoides ssp. sinensis groves and the surrounding grassy areas. Shrubs had higher P content than grasses, but lower C:P ratios in their leaves, litter, and roots. Similarly, shrubs had higher microbial P content than grasses, but lower microbial biomass C:P and N:P ratios in the soil. The larger microbial P stock in the 1 m of soil beneath shrubs responded to the larger root P stock there as well. Thus, both the plants and microbes acquired more P in shrubby areas than in grassy areas by accelerating P mineralization. The greater net production of available P in the topsoil and the synthesis of microbial P throughout the profile under shrubs increased the P solubility. Total P, inorganic P, and organic P stocks were lower under shrubs than under grasses in the top 1 m of soil. This decrease in soil P storage beneath shrubs is most likely attributable to P leaching due to higher P solubility, heavy rainfall, and larger soil gaps. Moreover, shrubs also had larger plant biomass P stock compared to grasses, and thus the depletion of P from the top 1 m of soil was further magnified via plant biomass removal. We concluded that shrubs increase P cycling to overcome the stoichiometric imbalance between their P requirement and the supply in the soil, and the fast P cycling under shrubs magnify P depletion within the rooted soil depth in alpine meadows.


Assuntos
Fósforo , Solo , Biomassa , Carbono , Ecossistema , Pradaria , Tibet
5.
Zhongguo Zhong Yao Za Zhi ; 45(12): 2817-2826, 2020 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-32627455

RESUMO

Zerumbone(ZER), one of humulane-type sesquiterpenoids, showed its unique advantage against tumor activities. The main underlying mechanisms include inhibiting the growth and proliferation of cancer cells, inducing apoptosis of cancer cells and differentiation of cancer cells, regulating immune function, inhibiting invasion and metastasis of cancer cells, and reversing multidrug resistance of cancer cells. Studies on ZER focusing its cytotoxic or anti-tumor is one of hot topic. Currently, with the increasing studies on ZER, the clarified mechanisms are getting rich. The present paper describes a summary of its anti-tumor mechanism of action and helps to provide significant reference to more in-depth research.


Assuntos
Antineoplásicos/farmacologia , Sesquiterpenos/farmacologia , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral
6.
Ying Yong Sheng Tai Xue Bao ; 31(7): 2415-2421, 2020 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-32715708

RESUMO

Selecting a herbicide suitable for broomcorn millet is a key issue in high efficiency and safe production of broomcorn millet and ecological environment improvement. We compared the control effects of 22 different herbicides on weeds as wells as their effects on growth and development of glutinous millet (Yumi 2) in the field. The results showed that, 1) soil applied Goyou, Butachlor, atrazine, and Benzipram, and stem- and leaf-applied WP mixture of tribenuron-methyl·carfentrazone-ethyl·MCPA-Na, Sigma Broad, Tengjing, Taoshi·Youxian and Kuofei had little phytotoxi-city, with broomcorn millet seedlings growing normally, and the other herbicides had phytotoxicities on broomcorn millet. 2) All the herbicides controlled weeds in the field to some extent, with better performance of soil applied herbicides than the stem- and leaf-applied ones, and affected plant height, functional leaf SPAD and spike weight per plant of broomcorn millet. 3) Compared with manual weeding, all herbicides reduced yields of glutinous millet. Compared with no herbicides application, some herbicides had yield-increasing effects. Among the soil applied herbicides, Go-you, Butachlor, atrazine, and Benzipram performed better in weeding control, increasing the yield of broomcorn millet by more than 60% compared with no herbicide control. Among the stem- and leaf-applied herbicides, Sigma Broad and WP mixture of tribenuron-methyl·carfentrazone-ethyl·MCPA-Na performed better in weeding control, increasing the yield of glutinous millet by more than 50% compared with no herbicide control. Therefore, applying 38% atrazine or 44% Monosulfuron to soil before the emergence of broomcorn millet, or stem- and leaf-applied 3.6% mesosulfuron-methyl or 55% WP mixture of tribenuron-methyl·carfentrazone-ethyl·MCPA-Na after the emergence of broomcorn millet, performed better in weeding control and with limited effects on the growth and development of broomcorn millet.


Assuntos
Herbicidas , Panicum , Plantas Daninhas , Solo , Controle de Plantas Daninhas
7.
Zhongguo Zhong Yao Za Zhi ; 45(9): 2063-2072, 2020 May.
Artigo em Chinês | MEDLINE | ID: mdl-32495555

RESUMO

Bawei Chenxiang Powder is a traditional Tibetan folk medicine formula, consisting of resinous wood of Aquilaria sinensis, kernel of Myristica fragrans, fruit of Choerospondias axillaris, travertine, resin of Boswellia carterii or B. bhaw-dajiana, stem of Aucklandia lappa, fruit of Terminalia chebula(roasted), and flower of Gossampinus malabarica. It has the function of clearing heart heat, nourishing heart, tranquilizing mind, and inducing resuscitation, which has been used for the treatment of coronary heart disease and angina pectoris. Modern research shows that the medicine materials of this formula mainly contain terpenoids like sesquiterpenes and triterpenes and polyphenols like flavonoids, lignans, and tannins, displaying some pharmacological activities such as anti-myocardial ischemia, anti-cerebral ischemia, and spatial learning and memory promotion. This review summaries the traditional uses, chemical constituents, and pharmacological activities research progress, hopefully to provide a reference for clarification of its pharmacological active ingredients.


Assuntos
Medicamentos de Ervas Chinesas , Terminalia , Flavonoides , Medicina Tradicional Tibetana , Tibet
8.
Cardiovasc Drugs Ther ; 34(1): 3-14, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-32103377

RESUMO

PURPOSE: We investigated whether increased expression of activated mitogen-activated protein kinase (MAPK) kinases 1 (MEK1) restores ischemic post-conditioning (IPostC) protection in hypertrophic myocardium following ischemia/reperfusion (I/R) injury. METHODS: C57Bl/6 mice received recombinant adeno-associated virus type 9 (rAAV9)-mediated activated MEK1 gene delivery systemically, then following the induction of cardiac hypertrophy via transverse aortic constriction for 4 weeks. In a Langendorff model, hypertrophic hearts were subjected to 40 min/60 min I/R or with IPostC intervention consisting of 6 cycles of 10 s reperfusion and 10 s no-flow before a 60-min reperfusion. Hemodynamics, infarct size (IS), myocyte apoptosis and changes in expression of reperfusion injury salvage kinase (RISK) pathway were examined. RESULTS: rAAV9-MEK1 gene delivery led to a 4.3-fold and 2.7-fold increase in MEK1 mRNA and protein expression in the heart versus their control values. I/R resulted in a larger IS in hypertrophic than in non-hypertrophic hearts (52.3 ± 4.7% vs. 40.0 ± 2.5%, P < 0.05). IPostC mediated IS reduction in non-hypertrophic hearts (27.6 ± 2.6%, P < 0.05), while it had no significant effect in hypertrophic hearts (46.5 ± 3.1%, P=NS) compared with the IS in non-hypertrophic or hypertrophic hearts subjected to I/R injury only, respectively. Hemodynamic decline induced by I/R was preserved by IPostC in non-hypertrophic hearts but not in hypertrophic hearts. rAAV9-MEK1 gene delivery restored IPostC protection in hypertrophic hearts evidenced by reduced IS (32.0 ± 2.8% vs. 46.5 ± 3.1%) and cardiac cell apoptosis and largely preserved hemodynamic parameters. These protective effects were associated with significantly increased phosphorylation of ERK1/2 and ribosomal protein S6 kinases (p70S6K), but it had no influence on Akt and glycogen synthase kinase-3ß. CONCLUSION: These results demonstrated that rAAV9-mediated activated MEK1 expression restores IPostC protection in the hypertrophic heart against I/R injury through the activation of ERK pathway.


Assuntos
Dependovirus/genética , Técnicas de Transferência de Genes , Vetores Genéticos , Hipertrofia Ventricular Esquerda/terapia , Pós-Condicionamento Isquêmico , MAP Quinase Quinase 1/biossíntese , Infarto do Miocárdio/prevenção & controle , Traumatismo por Reperfusão Miocárdica/prevenção & controle , Miocárdio/enzimologia , Animais , Apoptose , Modelos Animais de Doenças , Indução Enzimática , Hipertrofia Ventricular Esquerda/enzimologia , Hipertrofia Ventricular Esquerda/genética , Hipertrofia Ventricular Esquerda/patologia , Preparação de Coração Isolado , MAP Quinase Quinase 1/genética , Masculino , Camundongos Endogâmicos C57BL , Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Infarto do Miocárdio/enzimologia , Infarto do Miocárdio/genética , Infarto do Miocárdio/patologia , Traumatismo por Reperfusão Miocárdica/enzimologia , Traumatismo por Reperfusão Miocárdica/genética , Traumatismo por Reperfusão Miocárdica/patologia , Miocárdio/patologia , Fosforilação , Proteínas Quinases S6 Ribossômicas 70-kDa/metabolismo
9.
Zhongguo Zhong Yao Za Zhi ; 45(24): 5917-5928, 2020 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-33496131

RESUMO

Saussurea lappa originates in India, and now mainly grow in Yunnan, Sichuan and other places in China. It is one of the commonly used traditional herbal medicines in Tibet and other minority regions, with effects in regulating qi to relieve pain and invigo-rating spleen to promote food. It has been used in clinic for gastrointestinal diseases, such as Qi stagnation syndrome of spleen and stomach, diarrhea and tenesmus. More than 200 compounds have been identified from S. lappa. Among them, sesquiterpenoids attracted much attention. In terms of the number of compounds, eudesmanetype is dominant, guaiane and germacranetypes have also been reported frequently. Pharmacological studies have involved extracts, volatile oils and monomeric components represented by dehydrocostus lactone. Anti-tumor, anti-inflammatory and anti-bacterial effects on digestive system have attracted great attention. However, due to the complex sources of S. lappa and widely used in clinical practice, there is few research progress on relevant chemical constituents and pharmacological activities. This paper systematically summarizes terpenes and the pharmacological effects of S. lappa, in order to provide basis for further studies and clinical applications.


Assuntos
Saussurea , Sesquiterpenos , China , Extratos Vegetais , Terpenos , Tibet
10.
J Asian Nat Prod Res ; 21(12): 1161-1169, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31760800

RESUMO

Three new eremophilane-type sesquiterpenoids, alashanoids K-M (1-3), and one known analogue (4) were isolated from the peeled stems of Syringa pinnatifolia. All the compounds were isolated from the genus Syringa for the first time. Structures of these compounds were established using 1D and 2D NMR and MS data. The absolute configurations were determined by experimental and calculated electronic circular dichroism analysis, a modification of Mosher's method, and X-ray diffraction. Compounds 2 and 3 inhibited NO production in LPS-induced RAW264.7 macrophage cells with IC50 values of 14.23 and 12.20 µM, respectively, and showed cytotoxic activities against HepG2 cells with the IC50 values of 34.41 and 40.86 µM, respectively.


Assuntos
Sesquiterpenos , Syringa , Animais , Camundongos , Estrutura Molecular , Casca de Planta , Sesquiterpenos Policíclicos
11.
Can J Cardiol ; 35(10): 1366-1376, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31495686

RESUMO

BACKGROUND: The purpose of the study was to assess the value of admission macrophage migration inhibitory factor (MIF) levels in predicting clinical outcomes in ST-elevation myocardial infarction (STEMI) patients. METHODS: For this study we recruited 498 STEMI patients after they received percutaneous coronary intervention (PCI), 40 with stable angina pectoris and 137 healthy participants. Plasma MIF levels were measured at admission and after PCI. The primary end points were in-hospital mortality and major adverse cardio-and/or cerebrovascular events (MACCE) during hospitalization and 3.2-year follow-up period. RESULTS: Admission MIF levels were elevated in 88.4% of STEMI patients over the upper reference limit of healthy controls and it was 3- to 7-fold higher than that in stable angina pectoris and control groups (122 ± 61 vs 39 ± 19 vs 17 ± 8 ng/mL; P < 0.001). Admission MIF levels were significantly higher in patients who died after myocardial infarction vs survivors. For predicting in-hospital mortality using the optimal cutoff value (127.8 ng/mL) of MIF, the area under the receiver operating characteristic curve for MIF was 0.820, similar area under the receiver operating characteristic curve values for predicting short-term outcomes were observed for high-sensitivity troponin T, CK-MB, N-terminal probrain natriuretic peptide, and Global Registry of Acute Coronary Events (GRACE) score. Although peak high-sensitivity troponin T and N-terminal probrain natriuretic peptide also predicted MACCE during the follow-up period, only higher admission MIF levels predicted in-hospital mortality and MACCE during the 3.2-year follow-up. Multivariate regression analysis showed the independent predictive value of a higher admission MIF level (≥ 127.8 ng/mL) on in-hospital mortality (odds ratio, 9.1; 95% confidence interval, 1.7-47.2) and 3.2-year MACCE (hazard ratio, 2.8; 95% confidence interval, 1.5-5.6). CONCLUSIONS: A higher admission MIF level is an independent predictor for in-hospital mortality and long-term MACCE in STEMI patients who underwent PCI.


Assuntos
Oxirredutases Intramoleculares/sangue , Fatores Inibidores da Migração de Macrófagos/sangue , Intervenção Coronária Percutânea , Infarto do Miocárdio com Supradesnível do Segmento ST/sangue , Infarto do Miocárdio com Supradesnível do Segmento ST/cirurgia , Idoso , Feminino , Mortalidade Hospitalar , Humanos , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Prognóstico , Estudos Prospectivos , Infarto do Miocárdio com Supradesnível do Segmento ST/mortalidade , Resultado do Tratamento
12.
Zhongguo Dang Dai Er Ke Za Zhi ; 21(3): 203-207, 2019 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-30907340

RESUMO

OBJECTIVE: To investigate the level of IgG antibody to varicella in the healthy population aged 1-19 years in Harbin, China. METHODS: Random sampling was performed to select 1 203 healthy individuals aged 1-19 years in Harbin. According to age, they were divided into ≥1 years group (n=240), ≥4 years group (n=396), ≥7 years group (n=364) and 14-19 years group (n=203). Enzyme-linked immunosorbent assay was used to measure the concentration of varicella-zoster virus (VZV)-IgG antibody in serum, and a concentration of VZV-IgG antibody of ≥100 mIU/mL was considered positive, suggesting that the subject had the ability to resist VZV infection. RESULTS: The overall positive rate of VZV-IgG antibody was 71.49% (860/1 203), and the concentration of VZV-IgG antibody was 447±17 mIU/mL. The concentration of VZV-IgG antibody tended to increase with age (P<0.05). The positive rate of VZV-IgG antibody in the urban population was significantly higher than that in the rural population (P<0.05). There was significant difference in the positive rate of VZV-IgG antibody between the populations with different doses of varicella vaccination (P<0.05), and the population with 2 doses of vaccination had the highest positive rate of VZV-IgG antibody. There was a significant difference in the concentration of VZV-IgG antibody between the populations with different medical histories (P<0.05), and the population with a past history of varicella had the highest concentration. CONCLUSIONS: Among the healthy population aged 1-19 years in Harbin, there is a significant difference in the level of VZV-IgG antibody between the urban and rural populations, as well as between different age groups. Varicella vaccination should be strengthened in areas with a low vaccination rate and the population aged <14 years.


Assuntos
Varicela , Adolescente , Adulto , Anticorpos Antivirais , Varicela/epidemiologia , Criança , Pré-Escolar , China/epidemiologia , Herpesvirus Humano 3 , Humanos , Imunoglobulina G , Lactente , Estudos Soroepidemiológicos , Adulto Jovem
13.
Zhongguo Zhong Yao Za Zhi ; 43(19): 3811-3821, 2018 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-30453703

RESUMO

Usnic acid and its derivatives, a group of organic molecules with great importance, are characteristic to lichens, possessing pharmacological activities such as anti-virus, anti-bacteria, anti-humor, anti-inflammatory, analgesic, and anaesthetic effects. Many of them have been widely used as medicine, but also bring side effects such as dermatitis and liver damages. In the past decades, great efforts by isolation, organic synthesis, and structure modification methods were put on discovery of UA derivatives with higher biological activities or less side effects. This paper describes herein the most progress on natural sources, isolation and structure elucidation, structural characteristics, synthesis and modification results, pharmacological activities and toxicities of UA and its derivatives, hopefully to provide valuable reference for further research.


Assuntos
Benzofuranos/química , Benzofuranos/farmacologia , Líquens/química , Produtos Biológicos
14.
Chin J Dent Res ; 21(4): 275-284, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30264044

RESUMO

OBJECTIVE: To evaluate the use of oral health services, the economic burden of oral diseases and related influential factors in China. METHOD: Using the multistage, stratified, equal proportion, random sampling method in the 4th National Oral Health Survey of China conducted in 2015 to 2016, residents aged 3 to 5 years, 12 to 15 years, 35 to 44 years, 55 to 64 years, and 65 to 74 years respectively were recruited, clinically examined and answered a questionnaire. Utilisation of oral health services were assessed in all the age groups and the economic burden of oral diseases in the past 12 months were assessed in the 3 to 5 years and 35 to 74 year-old groups. Chi-squared tests, t tests, correlation analysis and a one-way ANOVA were used to determine the relationships of different factors with utilisation of oral health services and the economic burden of oral diseases. RESULTS: In the subject groups - 3 to 5 years, 12 to 15 years and 35 to 74 years - the prevalence of the utilisation of oral health services in the past 12 months was 14.6% (5,876/40,353), 23.6% (27,936/118,592), and 20.1% (2,708/13,461), respectively. In all three groups, receiving dental treatment was the most common reason for subjects' recent dental visit. The average dental cost in the past 12 months was 403.43 CNY (median = 100) for 3 to 5-year-old children and 850.83 CNY (median = 300) for adults aged 35 to 74 years old. Area, education and annual household income per person were the socio-economic influential factors. Oral health status, oral hygiene and attitudes to and knowledge of oral health affected the utilisation of oral health services and the economic burden of oral diseases. CONCLUSION: The percentage of dental service utilisation was relatively low, and the economic burden was high. The related factors for both utilisation of oral health services and the economic burden of oral diseases included living in area, educational attainment, household income, perceived oral health status, and oral hygiene.


Assuntos
Serviços de Saúde Bucal/estatística & dados numéricos , Custos de Cuidados de Saúde/estatística & dados numéricos , Saúde Bucal/estatística & dados numéricos , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , China/epidemiologia , Índice CPO , Dispositivos para o Cuidado Bucal Domiciliar , Serviços de Saúde Bucal/economia , Escolaridade , Feminino , Hemorragia Gengival/epidemiologia , Conhecimentos, Atitudes e Prática em Saúde , Nível de Saúde , Humanos , Masculino , Pessoa de Meia-Idade , Saúde Bucal/economia , Higiene Bucal/estatística & dados numéricos , População Rural , Escovação Dentária/estatística & dados numéricos , População Urbana
15.
Int Dent J ; 68(6): 378-385, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29740814

RESUMO

OBJECTIVES: Oral rehabilitation under general anaesthesia (GA), commonly employed to treat high caries-risk children, has been associated with high economic and individual/family burden, besides high post-GA caries recurrence rates. As there is no caries prediction model available for paediatric GA patients, this study was performed to build caries risk assessment/prediction models using pre-GA data and to explore mid-term prognostic factors for early identification of high-risk children prone to caries relapse post-GA oral rehabilitation. METHODS: Ninety-two children were identified and recruited with parental consent before oral rehabilitation under GA. Biopsychosocial data collection at baseline and the 6-month follow-up were conducted using questionnaire (Q), microbiological assessment (M) and clinical examination (C). RESULTS: The prediction models constructed using data collected from Q, Q + M and Q + M + C demonstrated an accuracy of 72%, 78% and 82%, respectively. Furthermore, of the 83 (90.2%) patients recalled 6 months after GA intervention, recurrent caries was identified in 54.2%, together with reduced bacterial counts, lower plaque index and increased percentage of children toothbrushing for themselves (all P < 0.05). Additionally, meal-time and toothbrushing duration were shown, through bivariate analyses, to be significant prognostic determinants for caries recurrence (both P < 0.05). CONCLUSIONS: Risk assessment/prediction models built using pre-GA data may be promising in identifying high-risk children prone to post-GA caries recurrence, although future internal and external validation of predictive models is warranted.


Assuntos
Anestesia Geral , Cárie Dentária , Conhecimentos, Atitudes e Prática em Saúde , Medição de Risco/métodos , Criança , Pré-Escolar , Cárie Dentária/diagnóstico , Cárie Dentária/prevenção & controle , Cárie Dentária/terapia , Humanos , Lactente , Higiene Bucal/educação , Pais , Educação de Pacientes como Assunto , Projetos Piloto , Recidiva , Saliva/microbiologia , Prevenção Secundária , Fatores Socioeconômicos , Inquéritos e Questionários
16.
Zhongguo Zhong Yao Za Zhi ; 42(19): 3676-3683, 2017 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-29235279

RESUMO

Meconopsis horridula is one of alpine plants belonging to family Papaveraceae, mainly distributed in Himalaya Range area. M. horridula is a rare alpine flower, and is a kind of traditional Tibetan medicine, which has been included in more than 40 compound formulae, having efficacies of clearing away heat and alleviating pain, activating blood circulation to remove stasis, traditionally used for the treatment of fractures, injuries, and chest and back pains. Modern research shows that the whole plant of M. horridula contains alkaloids, flavonoids, and terpenes, and its pharmacological activities including antitumor, antivirus and myocardial protection etc. However, due to various factors, the current research of M.horridula still faces many challenges. This paper summaries herein a progress of MH on its ecological resources, traditional uses, and studies on chemical constituents and pharmacological effects, hopefully to provide a useful reference for the ecological protection and applications.


Assuntos
Alcaloides/farmacologia , Flavonoides/farmacologia , Medicina Tradicional Tibetana , Papaveraceae/química , Compostos Fitoquímicos/farmacologia , Terpenos/farmacologia
17.
Colloids Surf B Biointerfaces ; 159: 580-585, 2017 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-28854414

RESUMO

Based on the structurally similar properties of progesterone and cholesterol, chitosan-coated cholesterol-free liposomes (CS-Lipo/Prog) were formulated. CS-Lipo/Prog are spherical and uniform in size (662.1±19.3nm) with positive potential (28.19±1.97mV). The average drug entrapment efficiency (EE) is approximately 80%. The in vitro release profile of CS-Lipo/Prog shows sustained release. The in vitro stability evaluation demonstrated that CS-Lipo/Prog can efficiently shield Prog from degradation in the gastrointestinal tract. CS-Lipo/Prog showed a longer MRT and higher AUC0-infinite after oral administration to mice than in the control group (progesterone-free). The relative bioavailability of CS-Lipo/Prog was higher than that of progesterone soft capsules (QINING®) and Lipo/Prog. Collectively, these findings suggest that cholesterol-free chitosan-coated liposomes are a promising alternative for improving the oral bioavailability of progesterone.


Assuntos
Quitosana/química , Colesterol/química , Lipossomos/química , Progesterona/química , Animais , Trato Gastrointestinal/metabolismo , Camundongos
18.
Zhongguo Zhong Yao Za Zhi ; 41(10): 1864-1869, 2016 May.
Artigo em Chinês | MEDLINE | ID: mdl-28895334

RESUMO

Sixteen compounds were isolated from lichen Usnea longissima using of various chromatographic techniques including silica gel, Sephadex LH-20, ODS, and semi-preparative HPLC. By spectroscopic data analyses, their structures were identified by as useanol(1), lecanorin(2), 3-hydroxy-5-methylphenyl 2-hydroxy-4-methoxy-6-methylbenzoate(3), lecanorin E(4), 3'-methylevernic acid(5), evernic acid(6), barbatinic acid(7), 3,7-dihydroxy-1,9-dimethyldibenzofuran(8), orcinol(9), O-methylorcinol(10), methyl orsellinate(11), methyl everninate(12), 2,5-dimethyl-1,3-benzenediol(13), 2-hydroxy-4-methoxy-3,6-dimethyl benzoic acid(14), ethyl everninate(15), and ethyl 2,4-dihydroxy-6-methylbenzoate(16). Compound 1 was obtained as a natural product for the first time, and 3,4, 8,10,12, and 13 were isolated from Usneaceae family for the first time. Compound 1, 8, and 13 showed significant anti-inflammatory activity against NO production in RAW 267.4 cells with IC50 values of 6.8, 3.9 and 4.8 µmol•L⁻¹, respectively, compared with the positive controls curcumin(IC50 15.3 µmol•L⁻¹) and indomethacin(IC50 42.9 µmol•L⁻¹).


Assuntos
Fenóis/isolamento & purificação , Usnea/química , Animais , Cromatografia Líquida de Alta Pressão , Camundongos , Células RAW 264.7
19.
Guang Pu Xue Yu Guang Pu Fen Xi ; 36(7): 2260-5, 2016 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-30036004

RESUMO

According to the commonly used method of analysis with ICP-AES in geochemistry, to study the influence factors of interference from the analysis results, standard soil substances were selected to be the calibration curve of work, and the same method of digestion with soil samples was used to balance and eliminate the matrix interference. The concentrations of major and minor elements in soil samples were measured; the relative deviation of the results was compared under conditions of soil matrix and non-soil matrix interference; the relations and laws were being analyzed. The relative deviation (RE%) of testing results under non-soil-matrix interference were found floating around zero, the ratios of positive deviation and negative deviation were almost the same. Excluding the factors of spectral interference, the method of matrix matching can effectively eliminate the effects of matrix interference on soil. It was found that the analysis results of major elements, such as Al, Ca, Fe, Mg, P, Ti and Ba, were influenced negatively greatly under the condition of soil matrix interference, The maximum deviation of Mg 279.5 nm was up to -14.49%. The degree of influence ranking showed as Ti, Mg>P, Fe>Ca, Ba>Al. However, there is no obvious effect on other elements, including Na, Cr, Cu, V, Li, Mn, Ni and Sr. Contrary to the original ideas, the matrix interference effected greatly on the results of elements of high content, nevertheless, the effects on minor elements were not significant. As to the comprehensive matrix interference , the large proportion of interference from component self-content appeared of elements of Ca and Mg, because obvious linear correlation was found between component self-content and the relative deviation of the testing results of Ca and Mg. But no linear trend appeared between the self-contents of other elements and the results of matrix interference, indicating that the influence weight from self-content of other elements was very small. It was very important to select the right spectral lines, and remove the factors of interference to determinate the results of measurement. Factors and rules of interference effect has always been the research topic by all of scholars in the research field of ICP spectrum. On the guidance of above research results, the spectral lines will be selected and the accuracy judged reasonably, when soil samples being analyzed by ICP-AES.

20.
Biochim Biophys Acta ; 1864(1): 20-8, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26472331

RESUMO

SRM (selected reaction monitoring), a tandem mass spectrometry-based method characterized by high repeatability and accuracy, is an effective tool for the quantification of predetermined proteins. In this study, we built a time-scheduled dimethyl-SRM method that can provide the precise relative quantification of 92 proteins in one run. By applying this method to the Salmonella PhoP/PhoQ two-component system, we found that the expression of selected PhoP/PhoQ-activated proteins in response to Mg(2+) concentrations could be divided into two distinct patterns. For the time-course SRM experiment, we found that the dynamics of the selected PhoP/PhoQ-activated proteins could be divided into three distinct patterns, providing a new clue regarding PhoP/PhoQ activation and regulation. Moreover, the results for iron homeostasis proteins in response to Mg(2+) concentrations revealed that the PhoP/PhoQ two-component system may serve as a repressor for iron uptake proteins. And ribosomal protein levels clearly showed a response to different Mg(2+) concentrations and to time.


Assuntos
Proteínas de Bactérias/metabolismo , Biologia Computacional/métodos , Proteômica/métodos , Salmonella/metabolismo , Espectrometria de Massas em Tandem/métodos , Western Blotting , Relação Dose-Resposta a Droga , Magnésio/farmacologia , Peptídeos/metabolismo , Mapas de Interação de Proteínas/efeitos dos fármacos , Proteoma/metabolismo , Fatores de Tempo
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