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1.
Gene ; 738: 144460, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32045659

RESUMO

MicroRNA390 (miR390), an ancient and highly conserved miRNA family in land plants, plays multiple roles in plant growth, development and stress responses. In this study, we isolated and identified MIR390, miR390, TAS3a/b/c, tasiARF-1/2/3 (trans-acting small interfering RNAs influencing Auxin Response Factors) and ARF2/3/4 in Jerusalem artichoke (Helianthus tuberosus L.). Treatment with 100 mM NaCl induced expression of miR390, increased cleavage of TAS3, produced high levels of tasiARFs, and subsequently enhanced cleavage of ARF3/4, which was most likely associated with salt tolerance of the plants. In contrast, treatment with 300 mM NaCl inhibited expression of miR390, attenuated cleavage of TAS3, produced a small amount of tasiARFs, and reduced cleavage of ARF3/4. We proposed that ARF2, one of the targets of tasiARFs, induced under salinity was likely to play an active role in salt tolerance of Jerusalem artichoke. The study of the miR390-TAS3-ARF model in Jerusalem artichoke may broaden our understanding of salt tolerance mechanisms, and provides a theoretical support for further genetic identification and breeding crops with increased tolerance to salt stress.

2.
J Sep Sci ; 43(4): 799-807, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31769594

RESUMO

Hydrosoluble trehalose lipid (a biosurfactant) was employed for the first time as a green extraction solution to extract the main antioxidant compounds (geniposidic acid, chlorogenic acid, caffeic acid, and rutin) from functional plant tea (Eucommia ulmoides leaves). Single-factor tests and response surface methodology were employed to optimize the extraction conditions for ultrasound-assisted micellar extraction combined with ultra-high-performance liquid chromatography in succession. A Box-Behnken design (three-level, three-factorial) was used to determine the effects of extraction solvent concentration (1-5 mg/mL), extraction solvent volume (5-15 mL), and extraction time (20-40 min) at a uniform ultrasonic power and temperature. In consequence, the best analyte extraction yields could be attained when the trehalose lipid solution concentration was prepared at 3 mg/mL, the trehalose lipid solution volume was 10 mL and the extraction time was set to 35 min. In addition, the recoveries of the antioxidants from Eucommia ulmoides leaves analyzed by this analytical method ranged from 98.2 to 102%. These results indicated that biosurfactant-enhanced ultrasound-assisted micellar extraction coupled with a simple ultra-high-performance liquid chromatography method could be effectively applied in the extraction and analysis of antioxidants from Eucommia ulmoides leaf samples.

3.
J Ethnopharmacol ; 242: 112055, 2019 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-31276751

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Dan-Lou tablet (DLT) is developed from the traditional Chinese medicine (TCM) formula Gualou Xiebai Baijiu Tang which has been used for at least 2000 years in China. DLT has been widely used in clinical practice to treat cardiovascular diseases. AIM OF THE STUDY: This study aimed to uncover the pharmacological mechanism of the compounds absorbed into the blood of Dan-Lou tablet (DLT) on coronary heart disease (CHD) using a network pharmacology integrated pharmacokinetics strategy. MATERIALS AND METHODS: A rapid and sensitive method was developed for the simultaneous determination of the six compounds (puerarin, formononetin, calycosin, paeoniflorin, cryptotanshinone and tanshinone IIA) in rat plasma by liquid chromatography tandem mass spectrometry (LC-MS/MS). Then, the pharmacology network was established based on the relationship between five compounds absorbed into the blood targets (puerarin, formononetin, calycosin, cryptotanshinone and tanshinone IIA) and CHD targets. RESULTS: The intra-and inter-day precision were less than 11% and the accuracy ranged from 88.2% to 112%, which demonstrated that the LC-MS/MS method could be used to evaluate the pharmacokinetic feature of the six compounds in rats after oral administration of DLT. The pathway enrichment analysis revealed that the significant bioprocess networks of DLT on CHD were positive regulation of estradiol secretion, negative regulation of transcription from RNA polymerase II promoter, lipopolysaccharide-mediated signaling pathway and cytokine activity. CONCLUSION: The proposed network pharmacology integrated pharmacokinetics strategy provides a combination method to explore the therapeutic mechanism of the compounds absorbed into the blood of multi-component drugs on a systematic level.


Assuntos
Doença das Coronárias/sangue , Medicamentos de Ervas Chinesas/farmacocinética , /sangue , Administração Oral , Animais , Cromatografia Líquida , Doença das Coronárias/metabolismo , Medicamentos de Ervas Chinesas/farmacologia , Glucosídeos/sangue , Glucosídeos/farmacocinética , Isoflavonas/sangue , Isoflavonas/farmacocinética , Masculino , Monoterpenos/sangue , Monoterpenos/farmacocinética , Miocárdio/metabolismo , Farmacologia/métodos , Fenantrenos/sangue , Fenantrenos/farmacocinética , Mapas de Interação de Proteínas , Ratos Sprague-Dawley , Espectrometria de Massas em Tandem
4.
Restor Neurol Neurosci ; 37(4): 375-395, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31282440

RESUMO

BACKGROUND: A cerebral ischemic stroke involves mitochondrial dysfunction, motor deficits, and paralysis; and Danhong injection (DHI) might possess mitochondrial protection and functional recovery in a stroke subject through promoting expression of parkin, a ubiquitin ligase playing a key role in the regulation of proteins and mitochondria quality control. OBJECTIVE: To investigate the therapeutic effects of DHI on the histological, cellular, and functional recovery of Wistar rats after middle cerebral artery occlusion/reperfusion (MCAO/R). METHODS: One hundred and twenty healthy male Wistar rats (250-300 g), were randomly assigned to six groups (twenty rats/group). Rats were subjected to 1 h MCAO/R and subsequently administered the intravenous doses of DHI (0.75, 1.5, and 3 mL/kg) to the respective groups (twice a day for 14 days). Unlike the other groups, the sham group received surgery without vessel occlusion. All the animals were tested for gait behavior using the CatWalk system. The body weight/survival rates were recorded daily for 14 days. The parkin protein expression of the brain tissue was quantified by immunohistochemistry analysis. Additionally, cultured cortical neurons were incubation with DHI or minocycline (MC) and then deprived of oxygen and glucose for 2 h (to resemble ischemic/reperfusion), followed by 4 h reoxygenation. Cellular and mitochondrial phenotypes were assayed by high content analysis. RESULTS: Neurological integrity and paw parameters of the animals were altered in the model group but significantly ameliorated by DHI administration. Also, the infarct volume and survival rate were significantly improved in DHI groups. DHI enhanced the expression of parkin protein in the brain and improved the relative mitochondrial reductase activity of the cultured neurons. CONCLUSIONS: The overall result shows that daily intervention with DHI provides neuroprotection and survival to improve gait motion in Wistar rats.

5.
J Food Biochem ; 43(4): e12807, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31353604

RESUMO

A simple miniaturized matrix solid-phase dispersion extraction was developed to analyze multiple antioxidant ingredients (neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, isoquercitrin, and astragalin) in Mori Fructus by ultra performance liquid chromatography coupled with an ultraviolet detector. 1-ethyl-3-methylimidazolium bromide was employed in the elution process as eco-friendly elution solvent. The effects of some factors on the extraction efficiency of antioxidants in Mori Fructus were optimized in detail, such as the type of adsorbent, the type of elution solvent, the concentration and volume of elution solvent, sample to adsorbent ratio, and the grinding time. The intra-day and inter-day precisions (RSD) were both below 5.0% and the recoveries of all analytes ranged from 93.5% to 98.3%. Compared with the traditional method, it was efficient, simple and environment-friendly for extracting the analytes by ionic liquid assisted trace ß-CD matrix solid-phase dispersion extraction method, due to shorter extraction time, less reagent and less sample consumption. The developed method was successfully used for extracting and determining the active compounds in Mori Fructus sample. PRACTICAL APPLICATIONS: The antioxidant ingredients play important roles in Mori Fructus because of their main pharmacological activities. Nowadays, a few analytical methods could be applied for extracting and analyzing these target compounds. But these methods not only required much extracting and analyzing time, but also need a great deal of organic reagent, which were not environmentally friendly. Thus, a green and simple miniaturized matrix solid phase dispersion extraction method was proposed for the analysis of these antioxidants in Mori Fructus, which was efficient to be employed for evaluating the quality of Traditional Chinese Medicine.

6.
J Sep Sci ; 42(14): 2426-2434, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31077572

RESUMO

An industrial MCM-41-miniaturized matrix solid-phase dispersion extraction coupled with response surface methodology was explored to determine L-epicatechin, typhaneoside, isorhamnetin-3-O-neohespeidoside, naringenin, kaempferol, and isorhamnetin in Pollen typhae by ultra-high performance liquid chromatography connected to a photodiode array detection. Several variables were optimized in detail, including mesh number of sieve, type of adsorbent, mass ratio of sample to adsorbent, grinding time, methanol concentration, and elution volume. Central composite design was applied to optimize the best conditions for the maximum yields of the total flavonoids. The results displayed a good linear relationship (R > 0.9992) and the recoveries ranged from 92.9 to 103% (RSD < 4.53%) of the six flavonoids. The optimal method with high efficiency and low consumption was obviously better than heating reflux and ultrasonic extraction. It was proven that the developed industrial MCM-41-miniaturized matrix solid-phase dispersion extraction coupled with simple ultra-high performance liquid chromatography method could be a rapid and efficient tool for extraction and determination of flavonoids in natural products.


Assuntos
Flavonoides/análise , Pólen/química , Dióxido de Silício/química , Extração em Fase Sólida , Cromatografia Líquida de Alta Pressão , Propriedades de Superfície
7.
Fitoterapia ; 136: 104155, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31028819

RESUMO

In the quest to search and discover bioactive compounds from nature, terpenoids have emerged as one of the most interesting and researched classes of compounds. Secoiridoid, a type of the terpenoid, has also been extensively studied, especially their chemical structures and pharmacological effects. Oleaceae is a family of woody dicotyledonous plants with broad economic and medicinal values. This family contains a large number of flavonoids, monoterpenoids, iridoids, secoiridoids and phenylethyl alcohols, of which the secoiridoids have various biological activities. The purpose of this review is to summarize the phytochemical and pharmacological of the secoiridoids (glycosides, aglycones, derivatives and dimers) in the Oleaceae family from 1987 to 2018. This review will also serve as a reference for further studies.


Assuntos
Iridoides/química , Iridoides/farmacologia , Oleaceae/química , Animais , Flavonoides , Glicosídeos , Humanos , Estrutura Molecular , Monoterpenos , Compostos Fitoquímicos/química , Compostos Fitoquímicos/farmacologia
8.
Artigo em Inglês | MEDLINE | ID: mdl-31015855

RESUMO

Embelin exhibits the broad bioactivities such as antitumor, antifertility, antidiabetic, anti-inflammatory, antioxidant, anticonvulsant, anxiolytic, antimicrobial, and hepatoprotective activity. In order to further understand the pharmacokinetic characteristics and oral bioavailability of embelin in vivo, the concentration of embelin in rat plasma was determined by a sensitive high-performance liquid chromatography with diode array detector (HPLC-DAD). The preparation of samples was accomplished by a simple precipitating protein with methanol. Emodin was selected as the internal standard (IS). Embelin and IS were completely separated on an analytical column (Extend-C18, 4.6 × 250 mm, 5 µm) using 0.1% phosphoric acid in methanol and 0.1% phosphoric acid in aqueous solution (90:10, v/v) as the mobile phase. The lower limit of quantification was 0.15 µg/mL. Oral bioavailability of embelin was 30.2 ± 11.9%. This study could provide the information about pharmacokinetics and oral bioavailability of embelin, which was useful to assess the clinic efficacy and safety and promote further development of embelin.

9.
Int Immunopharmacol ; 72: 204-210, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30999210

RESUMO

Acute pancreatitis (AP) is a common acute abdominal disease with local or systemic inflammatory response, caused by abnormal activation of digestive enzymes. Baicalein has been shown to exert anti-inflammatory effects and to attenuate the pathological changes of AP. The aim of the research was to investigate the effects of baicalein on caerulein induced pancreatitis, and to elucidate the putative underlying mechanism. In this study, the therapeutic potential of baicalein and its mechanism were investigated in a caerulein-induced AP in vivo and in vitro model. The results indicate that baicalein treatment alleviates the caerulein-induced pathological damage in the pancreas. Baicalein decreased the expression level of pro-inflammatory cytokines and chemokines of the pancreas in caerulein treated mice and of isolated pancreatic acinar cells. Moreover, baicalein inhibited the expression of NF-κB p65 and the phosphorylation of p38 MAPK, ERK (extracellular signal-regulated kinase) as well as STAT 3, which indicates that baicalein exerts its anti-inflammatory effects via dampening the NF-κB, MAPK and STAT 3 signaling pathways. Together, this study provides experimental evidence for the clinical application of Scutellaria baicalensis Georgi or baicalein and indicates that baicalein may be a promising candidate for treatment of AP patients in the future.


Assuntos
Anti-Inflamatórios , Flavanonas , Proteínas Quinases Ativadas por Mitógeno/metabolismo , NF-kappa B/metabolismo , Pancreatite , Fator de Transcrição STAT3/metabolismo , Células Acinares/efeitos dos fármacos , Células Acinares/metabolismo , Animais , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Sobrevivência Celular/efeitos dos fármacos , Ceruletídeo , Citocinas/metabolismo , Flavanonas/farmacologia , Flavanonas/uso terapêutico , Camundongos , Camundongos Endogâmicos C57BL , Pâncreas/efeitos dos fármacos , Pâncreas/metabolismo , Pâncreas/patologia , Pancreatite/induzido quimicamente , Pancreatite/tratamento farmacológico , Pancreatite/metabolismo , Pancreatite/patologia , Fitoterapia , Células RAW 264.7 , alfa-Amilases/metabolismo
10.
J Sep Sci ; 42(10): 1886-1895, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30828980

RESUMO

A novel ionic-liquid-based vortex-simplified matrix solid-phase dispersion method using 2,6-dimethyl-ß-cyclodextrin was established by ultra high performance liquid chromatography coupled with a photodiode array detector. 2,6-Dimethyl-ß-cyclodextrin was first used as a promising adsorbent in this proposed method for simultaneous determination of eight compounds in Gardeniae fructus. These compounds are terpenoids (geniposidic acid, genipin-1-ß-D-gentiobioside, geniposide, 8-o-acetyl shanzhiside methyl ester), crocins (crocin-I, crocin-II), quinic acid derivatives (chlorogenic acid), and flavonoids (isoquercitrin), respectively. Several parameters were investigated in the adsorption and desorption processes to obtain the optimal conditions, including 2,6-dimethyl-ß-cyclodextrin as sorbent, 0.5 mL 100 mM 1-dodecyl-3-methylimidazolium hydrogen sulfate as the extraction solvent, 2:1 of sample/sorbent ratio, grinding for 2 min and vortexing for 60 s. The recoveries of the eight compounds ranged from 96.6 to 100% (<3.50%). The limits of detection and quantification were in the range of 0.02-0.30 and 0.06-1.25  µg/mL, respectively. Meanwhile, a good linearity was attained with r values (>0.9997). The established method showed higher extraction efficiency and less reagent consumption than traditional matrix solid phase dispersion and ultrasonic-assisted extraction. Hence, it could be applied for sample preparation and analysis of natural products.


Assuntos
Carotenoides/análise , Flavonoides/análise , Gardenia/química , Ácido Quínico/análise , Terpenos/análise , China , Cromatografia Líquida de Alta Pressão , Química Verde , Humanos , Líquidos Iônicos/química , Íons/análise , Medicina Tradicional Chinesa , Compostos Orgânicos/análise , Farmacopeias como Assunto , Fenol/química , Controle de Qualidade , Extração em Fase Sólida , Solventes/química
11.
J Chromatogr A ; 1593: 147-155, 2019 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-30685187

RESUMO

An in-capillary 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)-sweeping micellar electrokinetic chromatography-diode array detector (ABTS+-sweeping MEKC-DAD) method was developed and successfully applied to screening and quantifying antioxidative ingredients from natural products. The parameters affecting sweeping and separation were optimized including components of background electrolyte and sample matrix. Comparing with previously reported MEKC, the sensitivity enhancement factors of trace natural antioxidants obtained by this proposed method were from 17 to 167. The limit of detection was as low as 6 ng·mL-1. The results of other validation parameters including linearity, reproducibility, accuracy and recovery were satisfactory. Seven compounds including schizandrin, schisandrol B, schisantherin B, schisantherin A, schisanhenol, deoxyschizandrin, schisandrin B were identified as the main antioxidants in Schisandra chinensis. It was demonstrated that this developed in-capillary ABTS+-sweeping MEKC-DAD is simple, sensitive, reliable and rapid method for screening and quantifying trace antioxidants from natural products.


Assuntos
Antioxidantes/análise , Cromatografia Capilar Eletrocinética Micelar/instrumentação , Cromatografia Capilar Eletrocinética Micelar/métodos , Lignanas/análise , Schisandra/química , Ácidos Sulfônicos/química , Limite de Detecção , Modelos Lineares , Extratos Vegetais/química , Reprodutibilidade dos Testes
12.
Nat Prod Res ; 33(8): 1162-1168, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29726268

RESUMO

Two pairs of new phenolic enantiomers, (+)-eucophenolic A (1a), (-)-eucophenolic B (1b), (-)-eucophenolic C (2a), (+)-eucophenolic D (2b) were isolated from the leaves of Eucommia ulmodies Oliver by chiral enantiomeric resolution. Their structures were elucidated based on extensive spectroscopic analysis. The absolute configurations of 1a/1b and 2a/2b were determined by empirical method and the calculated ECD and OR. All compounds were tested for Hep G2 tumour cell lines. However, no compounds showed potential cytotoxic activities against Hep G2 in vitro.


Assuntos
Eucommiaceae/química , Fenóis/isolamento & purificação , Folhas de Planta/química , Ensaios de Seleção de Medicamentos Antitumorais , Células Hep G2 , Humanos , Estrutura Molecular , Fenóis/análise , Fenóis/química , Fenóis/farmacologia , Análise Espectral , Estereoisomerismo
13.
J Asian Nat Prod Res ; 21(10): 985-991, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29996686

RESUMO

Two new labdane-type diterpenes, named viterotulin C (1) and vitexilactone D (2), together with five known diterpenes (3-7), were isolated from the fruits of Vitex trifolia L. var. simplicifolia Cham. Their structures were elucidated by detailed analysis of spectroscopic data. All the compounds were evaluated for their inhibitory effects on nuclear factor-kappa B (NF-κB) pathway in HEK 293 cell line. These compounds presented inhibition on TNF-α-induced NF-κB activation, with inhibition rates ranging from 42.52 ± 10.69% to 68.86 ± 10.76% at the concentration of 50 µM.

14.
J Chromatogr A ; 1584: 87-96, 2019 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-30473109

RESUMO

Large-scale targeted and untargeted metabolites characterization can be achieved by feat of different liquid chromatography/mass spectrometry (LC-MS) platforms by multiple MS experiments or using data-independent acquisition followed by precursor-product ions matching based on certain algorithms. The resulting insufficiency in efficiency and availability greatly restricts the applicability of these strategies in large-scale profiling and identification of various metabolites. A strategy simultaneously enabling both the targeted and untargeted metabolites characterization is established on a Q Exactive hybrid quadrupole-Orbitrap mass spectrometer, by integrating precursor ions list-triggered data-dependent MS2 acquisition (PIL/dd-MS2) of the targeted components and using the "If idle-pick others" (IIPO) function to induce untargeted metabolites fragmentation. A compounds-specific mass defect filter (MDF) algorithm is proposed as a method to generate the PIL. As a proof of concept, this strategy coupled with offline two-dimensional liquid chromatography (2D-LC) was applied to identify the multicomponents of a traditional Chinese medicine formula Erzhi Pill (EZP). A rigid MDF vehicle was elaborated by orthogonal screening of the integer mass and integer mass-dependent dynamic mass defects considering a variation of 20 ppm. The Full MS/dd-MS2 method enabling PIL and IIPO exhibited better performance than Full MS/dd-MS2 and Targeted SIM/dd-MS2 (selected ion monitoring) in respect of the sensitivity in identifying the targeted components and the ability to characterize more untargeted ones. As a consequence, 270 components were separated from EZP, and 146 thereof were selectively characterized. In conclusion, it is a practical, multifaced strategy facilitating the in-depth metabolites profiling and characterization of complex herbal and biological samples.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/análise , Medicamentos de Ervas Chinesas/química , Espectrometria de Massas em Tandem/métodos
15.
Biomed Chromatogr ; 33(3): e4433, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30414211

RESUMO

A sensitive and accurate LC-MS/MS method was established for quantifying bisabolangelone in rat plasma and tissues. Bisabolangelone was isolated and purified from Angelicae Pubescentis Radix. The pharmacokinetic and tissue distribution of bisabolangelone after administration to rat was performed by LC-MS/MS. Separation was carried out on a C8 (4.6 × 100 mm, 1.8 µm) column. The MS/MS transitions of bisabolangelone and tussilagone (internal standard) were set at m/z 249.1 → 109.1 and m/z 391.4 → 217.4, respectively. The lower limit of quantification in plasma and other tissues ranged from 1 to 4 ng/mL. The biosamples were prepared using protein precipitation method with acetonitrile. The recovery was >92%. The results showed that values of maximum concentrations and area under the curve depended linearly on the studied doses (2.5, 5 and 7.5 mg/kg body weight). The other ingredients in Angelicae Pubescentis Radix extract possibly reduce the absorption of bisabolangelone in rat. Tissue distribution revealed that bisabolangelone was widely distributed in vivo. The highest and lowest concentrations of bisabolangelone were found in the stomach and in the brain, respectively. It was concluded that the newly established HPLC-MS/MS method was suitable to describe the pharmacokinetic characteristics of bisabolangelone in rat after administration.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/farmacocinética , Sesquiterpenos/farmacocinética , Espectrometria de Massas em Tandem/métodos , Animais , Estabilidade de Medicamentos , Feminino , Modelos Lineares , Masculino , Ratos , Ratos Sprague-Dawley , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Sesquiterpenos/sangue , Sesquiterpenos/química , Distribuição Tecidual
16.
Bioorg Chem ; 81: 35-43, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30092385

RESUMO

Four sesquiterpenoid-chalcone hybrids (nardochalaristolones A-D, 1-4), a pair of epimeric sesquiterpenoid-flavonone hybrids ((2'S)- and (2'R)-nardoflavaristolone A, 5 and 6), and a sesquiterpenoid dimer (dinardokanshone F, 7), all sharing a kanshone C-derived sesquiterpenoid unit, were isolated from the underground parts of Nardostachys jatamansi (D.Don) DC. Their structures were elucidated by analysis of the extensive spectroscopic data, and the absolute configurations were established by analysis of 2D NMR spectroscopic data including NOESY data, combined with comparisons of experimental and calculated electronic circular dichroism spectra. Further, the plausible biosynthetic pathways for these compounds were proposed. And the results of SERT activity assay revealed that nardochalaristolones C-D (3 and 4) and nardoflavaristolone A (5 and 6) significantly enhanced SERT activity, while other compounds didn't show any SERT regulatory activities.


Assuntos
Chalcona/isolamento & purificação , Nardostachys/química , Sesquiterpenos/isolamento & purificação , Chalcona/química , Estrutura Molecular , Sesquiterpenos/química
17.
Front Pharmacol ; 9: 691, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30002628

RESUMO

Natural products, especially for traditional Chinese medicines (TCMs), are of great importance to cure diseases. Yet it was hard to screen the influential quality markers for monitoring the quality. A simple and comprehensive strategy was developed and validated to screen for the combinatorial quality markers for precise quality evaluation and discrimination of natural products. In this study, Pollen Typhae (PT) and it's processed products carbonized PT were selected as the representative case. Firstly, metabolomics data of 49 batches crude PT and carbonized PT was obtained by ultra high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS). Then, metabolomics approaches were performed to screen for the potential markers that lead to the quality difference. Finally, chemometric methods were used to validate the accuracy of combinatorial quality markers. Thus, 42 compounds were identified from PT, 5 markers (isorhamnetin-3-O-(2G-α-L-rhamnosyl)-rutinoside, isorhamnetin-3-O-neohesperidoside, astragalin, kaempferol and umbelliferone) were successfully screened, identified, quantified and regarded as combinatorial quality markers for precise quality evaluation of crude and carbonized PT. It was demonstrated that the established comprehensively strategy provide an efficient tool for precise quality evaluation of natural products from the whole.

18.
Electrophoresis ; 39(19): 2439-2445, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30027576

RESUMO

An on-line large volume sample stacking with polarity switching (LVSS) method was proposed for simultaneously determining lignanoids and ginsenosides in MEEKC. The parameters including the pH value and concentration of buffer solution, SDS, organic modifier, oil phase, running voltage, and temperature as well as injection time, sample matrix, stacking voltage, and time influencing separation and stacking were systematically optimized. The method was verified by performing precision, accuracy, stability, and recovery. Its reliability was proved by separating and quantifying two lignanoids and three ginsenosides in Shengmai injectionSMI. The sensitivity of these compounds was improved by MEEKC-LVSS method for 6-11 times than conventional MEEKC. Thus, this developed on-line MEEKC-LVSS method was sensitive, practical, and reliable.


Assuntos
Cromatografia Capilar Eletrocinética Micelar/métodos , Ginsenosídeos/análise , Lignanas/análise , Emulsões/química , Ginsenosídeos/química , Ginsenosídeos/isolamento & purificação , Lignanas/química , Lignanas/isolamento & purificação , Limite de Detecção , Modelos Lineares , Reprodutibilidade dos Testes
19.
J Asian Nat Prod Res ; 20(11): 1045-1054, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29996684

RESUMO

Two pairs of phenylpropanoid enantiomers, (+)-(7S,8S)-alatusol D (1a), (-)-(7R,8R)-alatusol D (1b), (-)-(7S,8R)-alatusol D (2a) and (+)-(7R,8S)-alatusol D (2b) were isolated from the leaves of Eucommia ulmoides Oliver. Among them, 1a and 2b were firstly obtained by chiral enantiomeric resolution. Their structures were elucidated based on extensive spectroscopic analysis and the induced CD (ICD) spectrum caused by adding Mo2(AcO)4 in DMSO. All compounds were tested on Hep G2 tumor cell lines. However, none of the compounds showed potential cytotoxic activity against Hep G2 in vitro.


Assuntos
Eucommiaceae/química , Folhas de Planta/química , Propanóis/farmacologia , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Sobrevivência Celular , Células Hep G2 , Humanos , Estrutura Molecular , Propanóis/química
20.
J Pharm Biomed Anal ; 156: 349-357, 2018 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-29753281

RESUMO

This study aimed to clarify the difference between the effective compounds of raw and processed Farfarae flos using a network pharmacology-integrated metabolomics strategy. First, metabolomics data were obtained by ultra high-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UHPLC-Q-TOF/MS). Then, metabolomics analysis was developed to screen for the influential compounds that were different between raw and processed Farfarae flos. Finally, a network pharmacology approach was applied to verify the activity of the screened compounds. As a result, 4 compounds (chlorogenic acid, caffeic acid, rutin and isoquercitrin) were successfully screened, identified, quantified and verified as the most influential effective compounds. They may synergistically inhibit the p38, JNK and ERK-mediated pathways, which would induce the inhibition of the expression of the IFA virus. The results revealed that the proposed network pharmacology-integrated metabolomics strategy was a powerful tool for discovering the effective compounds that were responsible for the difference between raw and processed Chinese herbs.


Assuntos
Descoberta de Drogas/métodos , Medicamentos de Ervas Chinesas/análise , Vírus da Influenza A/efeitos dos fármacos , Influenza Humana/tratamento farmacológico , Tussilago/química , Ácidos Cafeicos/análise , Ácidos Cafeicos/farmacologia , Ácido Clorogênico/análise , Ácido Clorogênico/farmacologia , Cromatografia Líquida de Alta Pressão/métodos , Sinergismo Farmacológico , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Flores/química , Humanos , Vírus da Influenza A/fisiologia , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Metabolômica/métodos , Quercetina/análogos & derivados , Quercetina/análise , Quercetina/farmacologia , Rutina/análise , Rutina/farmacologia , Biologia de Sistemas/métodos , Espectrometria de Massas em Tandem/métodos
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