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1.
Diabetes Care ; 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32033992

RESUMO

OBJECTIVE: To investigate the role of epigenetics in statins' diabetogenic effect comparing DNA methylation (DNAm) between statin users and nonusers in an epigenome-wide association study in blood. RESEARCH DESIGN AND METHODS: Five cohort studies' participants (n = 8,270) were classified as statin users when they were on statin therapy at the time of DNAm assessment with Illumina 450K or EPIC array or noncurrent users otherwise. Associations of DNAm with various outcomes like incident type 2 diabetes, plasma glucose, insulin, and insulin resistance (HOMA of insulin resistance [HOMA-IR]) as well as with gene expression were investigated. RESULTS: Discovery (n = 6,820) and replication (n = 1,450) phases associated five DNAm sites with statin use: cg17901584 (1.12 × 10-25 [DHCR24]), cg10177197 (3.94 × 10-08 [DHCR24]), cg06500161 (2.67 × 10-23 [ABCG1]), cg27243685 (6.01 × 10-09 [ABCG1]), and cg05119988 (7.26 × 10-12 [SC4MOL]). Two sites were associated with at least one glycemic trait or type 2 diabetes. Higher cg06500161 methylation was associated with higher fasting glucose, insulin, HOMA-IR, and type 2 diabetes (odds ratio 1.34 [95% CI 1.22, 1.47]). Mediation analyses suggested that ABCG1 methylation partially mediates the effect of statins on high insulin and HOMA-IR. Gene expression analyses showed that statin exposure and ABCG1 methylation were associated with ABCG1 downregulation, suggesting epigenetic regulations of ABCG1 expression. Further, outcomes insulin and HOMA-IR were significantly associated with ABCG1 expression. CONCLUSIONS: This study sheds light on potential mechanisms linking statins with type 2 diabetes risk, providing evidence on DNAm partially mediating statins' effects on insulin traits. Further efforts shall disentangle the molecular mechanisms through which statins may induce DNAm changes, potentially leading to ABCG1 epigenetic regulation.

2.
J Appl Genet ; 61(1): 37-50, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31912452

RESUMO

Wheat stripe rust can cause considerable yield losses, and genetic resistance is the most effective approach for controlling the disease. To identify the genomic regions responsible for Puccinia striiformis f. sp. tritici (Pst) resistance in a set of winter wheat strains mainly from southwestern China, and to identify DNA markers in these regions, we carried out a genome-wide association study (GWAS) of 120 China winter wheat accessions using single nucleotide polymorphism (SNP) markers from 90K wheat SNP arrays. In total, 16 SNP loci were significantly associated with wheat stripe rust in field and greenhouse trials. Of these, three distinctive SNPs on chromosomes 1B, 4A, and 6A were identified at a site in Mianyang in 2014, where the most prevalent wheat stripe rust races since 2009 have been V26 (G22-9, G22-14). This suggests that the three SNP loci were linked to the new quantitative trait loci (QTL)/genes resistant to the V26 races. Germplasm with immunity to Pst is a good source of stripe rust resistance for breeding, and after further validation, SNPs closely linked to resistance QTLs/genes could be converted into user-friendly markers and facilitate marker-assisted selection to improve wheat stripe rust resistance.

3.
Small ; 16(5): e1905842, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31916666

RESUMO

Rechargeable Zn/MnO2 batteries using mild aqueous electrolytes are attracting extensive attention due to their low cost, high safety, and environmental friendliness. However, the charge-storage mechanism involved remains a topic of controversy so far. Also, the practical energy density and cycling stability are still major issues for their applications. Herein, a free-standing α-MnO2 cathode for aqueous zinc-ion batteries (ZIBs) is directly constructed with ultralong nanowires, leading to a rather high energy density of 384 mWh g-1 for the entire electrode. Greatly, the H+ /Zn2+ coinsertion mechanism of α-MnO2 cathode for aqueous ZIBs is confirmed by a combined analysis of in situ X-ray diffractometry, ex situ transmission electron microscopy, and electrochemical methods. More interestingly, the Zn2+ -insertion is found to be less reversible than H+ -insertion in view of the dramatic capacity fading occurring in the Zn2+ -insertion step, which is further evidenced by the discovery of an irreversible ZnMn2 O4 layer at the surface of α-MnO2 . Hence, the H+ -insertion process actually plays a crucial role in maintaining the cycling performance of the aqueous Zn/α-MnO2 battery. This work is believed to provide an insight into the charge-storage mechanism of α-MnO2 in aqueous systems and paves the way for designing aqueous ZIBs with high energy density and long-term cycling ability.

4.
Bioresour Technol ; 297: 122456, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31786033

RESUMO

The addition of exogenous microorganisms is one approach with potential that may also overcome the problem in northern China of slow composting in autumn and winter due to low environmental temperatures. This study investigated the use of supplements of Lactobacillus plantarum (L. plantarum), strains P-8 and LP-10, on the efficiency of sheep manure composting and the quality of the final product. The composting process lasted eight weeks and, during this time, changes in multiple physical-chemical parameters and the compost microbiome were monitored. Microbiota-encoded functions, community structure and physical-chemical parameters were distinct between the two groups. 'Composting microbiota maturation index' was proposed to quantitatively compare the impact of maturation on composting microecology. The rapid improvement in composting rate (4 weeks) and quality of the final product suggest that this approach could provide both technological and economic benefits. This work reveals the tremendous potential of L. plantarum as a promoter in composting.


Assuntos
Compostagem , Lactobacillus plantarum , Animais , China , Esterco , Ovinos , Solo
5.
J Cell Biochem ; 121(3): 2618-2631, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31692054

RESUMO

Acinar cells in acute pancreatitis (AP) die through apoptosis and necrosis, the impacts of which are quite different. Early clinical interference strategies on preventing the progress of AP to severe acute pancreatitis (SAP) are the elimination of inflammation response and inhibition of necrosis. Muscarinic acetylcholine receptor M3 was encoded by Chrm3 gene. It is one of the best-characterized receptors of pancreatic ß cells and regulates insulin secretion, but its function in AP remains unclear. In this study, we explored the function of Chrm3 gene in the regulation of cell death in l-arginine-induced SAP animal models. We found that Chrm3 was upregulated in pancreatitis, and we further confirmed the localization of Chrm3 resided in both pancreatic islets and acinar cell membranes. The reduction of Chrm3 decreased the pathological lesion of SAP and reduced amylase activities in serum. Consistently, Chrm3 can suppress acinar cells necrosis markedly, but has no effect on regulating apoptosis after l-arginine treatment. It was shown that Chrm3 attenuated acinar cells necrosis at least in part by stabilizing caspase-8. Thus, this study indicates that Chrm3 is critical participants in SAP, and regulation of Chrm3 expression might be a useful therapeutic strategy for preventing pathologic necrosis.

6.
Biomark Med ; 14(1): 53-63, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31729251

RESUMO

Aim: This study profiled differentially expressed long noncoding RNAs (lncRNAs) in lung squamous cell carcinoma (LSCC) to predict LSCC overall survival (OS) using The Cancer Genome Atlas data. Materials & methods: The RNA-seq and clinical dataset of 475 LSCC patients was retrieved from The Cancer Genome Atlas database and statistically analyzed. Results: There were 67 upregulated and 32 downregulated lncRNAs in LSCCs and 12 lncRNAs associated with OS. The seven-lncRNA signature was associated with poor OS and RP11-150O12.6 and CTA-384D8.35 were associated with better OS (p < 0.001). The seven lncRNAs-mRNA interaction network analysis showed their association with 187 protein-coding genes for cancer development, cell migration, adhesion, proliferation, apoptosis, angiogenesis and the MAPK signaling pathways. Conclusion: This seven-lncRNA signature is useful to predict LSCC OS.

7.
J Hazard Mater ; 386: 121645, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31740314

RESUMO

Nitrocellulose (NC) has been applied in many fields of daily life and military industry, but its instability brings large danger during storage and usage, which greatly limits its application. In this study, decomposition behavior and reaction model of NC were explored. To obtain its kinetic triplets during decomposition, NC samples were heated in nitrogen atmosphere with different heating rates of 5, 10, 15, 20 °C min-1, respectively. A 3-th order reaction model was obtained by model fitting methods. Meanwhile, the relationship between activation energy and conversion was obtained by multi isoconversional methods, including KAS, FR, FWO, and Advanced Vyazovkin methods. Then the reaction model was reconstructed by introducing adjustment functions with result of an expression of f(α) = 19.38193α1.41177(1-α)4.60503.

8.
Arch Microbiol ; 202(1): 191-196, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31595323

RESUMO

A novel Gram-negative, aerobic, rod-shaped bacterium, RS19T, was isolated from rose rhizosphere soil. The strain was psychrophilic and showed good growth over a temperature range of 1-37 â„ƒ. Colonies on TSB agar were circular, smooth, mucoid, convex with clear edges and yellow. Phylogenetic analysis based on 16S rRNA gene sequences characterized RS19T in the genus Dyadobacter and showed that strain RS19T was most closely related to Dyadobacter psychrophilus CGMCC 1.8951T (97.4%) and Dyadobacter alkalitolerans CGMCC 1.8973T (97.1%). The average nucleotide identity values to the closest related species type strains were less than 84.0%. The DNA G + C content was 43.1 mol%, and the predominant respiratory menaquinone was MK-7. The major fatty acids were summed features 3 (C16:1ω7c and/or C16:1ω6c), iso-C15:0, C16:1ω5c and iso-C17:0 3-OH. Based on genotypic, phenotypic and chemotaxonomic data, strain RS19T is different from closely related species of the genus Dyadobacter. RS19T represents a novel species within the genus Dyadobacter, for which the name Dyadobacter luteus sp. nov. is proposed. The type strain is RS19T (= CGMCC 1.13719T = ACCC 60381T = JCM 32940T).


Assuntos
Cytophagaceae/classificação , Filogenia , Rizosfera , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases , Cytophagaceae/química , Cytophagaceae/genética , DNA Bacteriano/genética , Ácidos Graxos/análise , RNA Ribossômico 16S/genética , Rosa/microbiologia , Análise de Sequência de DNA , Especificidade da Espécie , Vitamina K 2/análise
9.
Surg Endosc ; 34(1): 384-395, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30972621

RESUMO

BACKGROUND: The number of publications of systematic reviews and meta-analyses (MAs) on robotic surgery have been increasing, including many investigating the same topic. Their quality and extent of overlap remains unclear. We assessed the quality of the MAs in this area and investigated the extent of their overlap. METHODS: Relevant studies were identified by searching the MEDLINE, EMBASE, and Cochrane Library databases up to August 1, 2017. Reporting and methodological quality levels were assessed using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) and Assessment of Multiple Systematic Reviews (AMSTAR) checklists. A thorough investigation of the extent of overlap was performed. RESULTS: In total, 90 MAs in 5 surgical subspecialties were included after full-text review. The mean reporting and methodological quality scores were 22.5 (83.2%) and 7.6 (69.2%), respectively. Authors from university-affiliated institutions and the presence of statistician or epidemiologist coauthors were associated with better-reporting quality scores. The topics with the most overlapping MAs (all ≥ 6) were robot-assisted thyroidectomy, prostatectomy, gastrectomy, colectomy, and fundoplication. 36 (40%) of the included MAs cited previous MAs on the same topic. Among the 7 MAs comparing robot-assisted radical prostatectomy to the open procedure, most (6/7) drew the same conclusion. 50 to 86% of MAs on this topic included the same trials as primary studies. CONCLUSION: Conducting multiple overlapping MAs with identical conclusions on the same topic that are of suboptimal quality may be a waste of resource and effort. Authors from university-affiliated institutes and experts in epidemiology and statistics are more likely to conduct MAs that have better quality. More guidelines and registries are needed to avoid overlapping MAs.

11.
Sci Total Environ ; 712: 135795, 2020 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-31866048

RESUMO

Although there have been many studies on filamentary bulking of activated sludge, it has been revealed that there is still a gap in the microscopic mechanism of filamentary bulking. In this study, the surface characteristics and thermodynamics of sludge floc were investigated to reveal the mechanism of filamentous sludge bulking. The results showed that the EPS content gradually decreased from 71.09 mg/g VSS to 40.00 mg/g VSS and the protein (PN) content of the EPS significantly decreased from 64.10 mg/g VSS to 35.85 mg/g VSS during sludge bulking. The variation in the EPS and its components led to a decrease in the relative hydrophobicity of sludge and an increase in surface negative charge; then, deterioration of the flocculation setteability of sludge flocs occurred. The electric double layer (WR) was the main force determining the aggregation of sludge during sludge bulking. The total energy of the interaction (WT) increased during sludge bulking, which led to an increase in the difficulty of sludge accumulation. This study is useful for understanding the filamentous bulking of sludge within an activated sludge process.

12.
ACS Appl Mater Interfaces ; 12(2): 2432-2444, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31845791

RESUMO

Discovering cathode materials composed of earth-abundant elements has become the current priority for developing sodium-ion batteries (SIBs) to meet the ever-increasing demand of large-scale energy storage. Herein, for the first time, layered NaxMO2 (M = Cu, Fe, Mn) cathodes are successfully prepared by directly using concentrated chalcopyrite ores as precursors. Greatly, impurity elements like Si and Ca are found to be crucial to tailoring the phase structure of as-obtained layered oxides as a P2 or O3 type, which removes the traditional concern that the impurities may restrict the utilization of natural ores. More interestingly, a certain amount of the Ca elements remaining in the Na sites through a self-doping process endows the P2-type products with enhanced structural stability. In half-cells, P2-type NaxMO2 with self-doped Ca elements shows superior rate capability and cycling stability (56 mAh g-1 at 5 C and 90% capacity retention after 100 cycles at 1 C). In contrast, less impurity elements are favorable for O3-type oxides to achieve a high capacity of 107 mAh g-1 at 0.1 C and 84% capacity retention after 200 cycles at 2 C. This new strategy would efficiently shorten the process for preparing electrode materials and open a feasible route to construct cheap and durable SIBs.

13.
Nanotechnology ; 31(14): 145203, 2020 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-31860897

RESUMO

In this paper, we fabricate ordered pore array (OPA) Ag film coated glass with the aid of polystyrene sphere (PS) array templates. This kind of OPA Ag coated glass has optical advantages of visible transparency, blue and near-infrared resistance. The average visible transmittance is 68%, including a transmission peak of 78% located at 570 nm, and low average transmittance of 48% in the blue light region that is not damaging to the eyes. The near-infrared light blocking rate is 67%, among which 40% light is reflected directly, indicating the reflection domination.

14.
Chin Med J (Engl) ; 133(2): 127-133, 2020 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-31880744

RESUMO

BACKGROUND: The role of local treatment in oligometastatic prostate cancer (PCa) is gaining interest with the oligometastases hypothesis proposed and the improvement of various surgical methods and techniques. This study aimed to compare the short-term therapeutic outcomes of robotic-assisted laparoscopic radical prostatectomy (RALP) for oligometastatic prostate cancer (OPC) vs. localized PCa using propensity score matching. METHODS: Totally 508 consecutive patients underwent RALP as a first-line treatment. The patients were divided into two groups according to oligometastatic state: the OPC group (n = 41) or the localized PCa group (n = 467). Oligometastatic disease was defined as the presence of two or fewer suspicious lesions. The association between the oligometastatic state and therapeutic outcomes of RALP was evaluated, including biochemical recurrence (BCR) and overall survival (OS). A Cox proportional hazards model was used to assess the possible risk factors for BCR. RESULTS: Totally 41 pairs of patients were matched. The median operative time, the median blood loss, the overall positive surgical margin rate, the median post-operative hospital stays, and the post-operative urinary continence recovery rate between the two groups showed no statistical significance. The 4-year BCR survival rates of the OPC group and localized PCa group were 56.7% and 60.8%, respectively, without a significant difference (P = 0.804). The 5-year OS rates were 96.3% and 100%, respectively (P = 0.326). Additionally, the results of Cox regression showed that oligometastatic state was not an independent risk factor for BCR (P = 0.682). CONCLUSIONS: Our findings supported the safety and effectiveness of RALP in OPC. Additionally, oligometastatic state and sites did not have an adverse effect on BCR independently.

15.
Urol Oncol ; 38(1): 2.e11-2.e17, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31672485

RESUMO

OBJECTIVE: Docetaxel-based chemotherapy remains the first-line treatment for patients with metastatic castration-resistant prostate cancer (mCRPC) in China. We have previously shown that time to nadir (TTN) of prostate-specific antigen (PSA) is an important prognostic factor in patients from a single center in Northwestern China. In this study, we performed a multicenter validation of the prognostic role of TTN in additional Chinese patients with mCRPC receiving docetaxel treatment. MATERIALS AND METHODS: The data were gathered from 170 eligible Chinese patients who received docetaxel chemotherapy from January 2007 to October 2018 in 11 Chinese Prostate Cancer Consortium member hospitals in China. TTN was defined as the time from start of chemotherapy to the nadir of PSA level during the treatment. Multivariable Cox regression models and Kaplan-Meier analysis were used to predict overall survival (OS) and progression-free survival (PFS). RESULTS: Patients with a TTN ≥ 15 weeks had a longer OS and PFS compared to those with a TTN < 15 weeks (43 vs. 15 months, P < 0.001; 24 vs. 6 months, P < 0.001, respectively). In addition, Patients with a TTN ≥ 15 weeks and PSA nadir <4.55ng/ml were associated with longer OS than others (HR 0.093, 95% CI 0.044-0.188, P < 0.001; HR 4.002, 95% CI 1.890-8.856, P = 0.001, respectively) and TTN, PSA nadir, PSA baseline (optimal threshold 56.07 ng/ml), and PSA reduction (optimal threshold 50%) were associated with PFS (HR 0.238, 95% CI 0.149-0.382, P < 0.001; HR 1.676, 95% CI 1.033-2.722, P = 0.037; HR 1.770, 95% CI 1.134-2.763, P = 0.012; HR 0.573, 95% CI 0.428-0.756, P < 0.001; respectively). Furthermore, patients with a PSA nadir <4.55 ng/ml had longer OS and PFS compared to other patients when TTN was ≥15 weeks. CONCLUSION: In this multicenter validation study, TTN and PSA nadir remain important prognostic markers in predicting therapeutic outcomes in Chinese men who receive chemotherapy for mCRPC.

16.
Environ Pollut ; : 113690, 2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31818625

RESUMO

Current studies indicate that long-term exposure to ambient fine particulate matter (PM2.5) is related with global mortality, yet no studies have explored relationships of PM2.5 and its species with DNAm PhenoAge acceleration (DNAmPhenoAccel), a new epigenetic biomarker of phenotypic age. We identified which PM2.5 species had association with DNAmPhenoAccel in a one-year exposure window in a longitudinal cohort. We collected whole blood samples from 683 elderly men in the Normative Aging Study between 1999 and 2013 (n = 1254 visits). DNAm PhenoAge was calculated using 513 CpGs retrieved from the Illumina Infinium HumanMethylation450 BeadChip. Daily concentrations of PM2.5 species were measured at a fixed air-quality monitoring site and one-year moving averages were computed. Linear mixed-effect (LME) regression and Bayesian kernel machine (BKM) regression were used to estimate the associations. The covariates included chronological age, body mass index (BMI), cigarette pack years, smoking status, estimated cell types, batch effects etc. Benjamini-Hochberg false discovery rate at a 5% false positive threshold was used to adjust for multiple comparison. During the study period, the mean DNAm PhenoAge and chronological age in our subjects were 68 and 73 years old, respectively. Using LME model, only lead and calcium were significantly associated with DNAmPhenoAccel. For example, an interquartile range (IQR, 0.0011 µg/m3) increase in lead was associated with a 1.29-year [95% confidence interval (CI): 0.47, 2.11] increase in DNAmPhenoAccel. Using BKM model, we selected PM2.5, lead, and silicon to be predictors for DNAmPhenoAccel. A subsequent LME model showed that only lead had significant effect on DNAmPhenoAccel: 1.45-year (95% CI: 0.46, 2.46) increase in DNAmPhenoAccel following an IQR increase in one-year lead. This is the first study that investigates long-term effects of PM2.5 components on DNAmPhenoAccel. The results demonstrate that lead and calcium contained in PM2.5 was robustly associated with DNAmPhenoAccel.

17.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 37(6): 642-647, 2019 Dec 01.
Artigo em Chinês | MEDLINE | ID: mdl-31875444

RESUMO

OBJECTIVE: This study evaluates the effects of different endodontic access methods and full-ceramic crown on the stress distribution in the maxillary central incisor by using three-dimensional finite element analysis. METHODS: Computed tomography scans of the maxillary central incisor were used to construct a three-dimensional finite element model of the maxillary central incisor. According to the different methods of endodontic and the prosthetic treatments, four models were established, namely, group A (traditional access cavity preparation with resin filling), group B (traditional access cavity preparation restored full-ceramic crown), group C (minimally invasive endodontics with resin filling) and group D (minimally invasive endodontics restored full-ceramic crown). A static force of 100 N and a direction of 45° was applied to the long axis of the tooth at the junction of the incisal section one-third and middle section one-third. The maximum principal stress, the von Mises stress and the modified von Mises stress of the tooth tissue were analyzed using the finite-element analysis software. RESULTS: 1) Stress peaks: the stress peaks of the maximum principal stress, the von Mises stress and the modified von Mises in group A were the largest, except that the stress peak of von Mises stress in group D was slightly lower than that in group C. The stress peaks of the maximum principal stress and the modified von Mises in group C were the lowest. The stress peaks of the maximum principal stress and the modi-fied von Mises stress in group D were lower than those in groups A and B. 2) Stress distribution: compared with group A, the stress distribution of cervical dentin and the area of stress concentration in group C was lower and smaller. In the root dentin, the stress distribution in group C was more uniform than that in group A, and the stress was dispersed to several areas of the root apex. After crown restoration, no significant difference was observed in stress distribution between groups B and D in the root region. The stress distribution state of group B was not significantly different from that of group A. No significant difference was observed in the stress distribution state between groups D and C. CONCLUSIONS: 1) From the perspective of biomechanics, the minimally invasive access was adopted for the maxillary central incisor. 2) Full crown restoration is recommended after traditional access cavity preparation. No obvious advantage is observed in stress analysis for minimally invasive endodontics-restored full-ceramic crown.


Assuntos
Incisivo , Técnica para Retentor Intrarradicular , Coroas , Análise do Estresse Dentário , Dentina , Análise de Elementos Finitos , Estresse Mecânico , Coroa do Dente
18.
Zhongguo Gu Shang ; 32(12): 1156-1159, 2019 Dec 25.
Artigo em Chinês | MEDLINE | ID: mdl-31870078

RESUMO

OBJECTIVE: To investigate the long-term effect of posterior lumbar pedicle screw fixation combined with isthmus bone grafting and fusion in young patients with spondylolysis. METHODS: A retrospective study was carried out, consisting of 16 young patients with lumbar spondylolysis without spondylolisthesis treated by lumbar posterior pedicle screw fixation combined with isthmic bone grafting fusion from January 2006 to July 2014. There were 11 males and 5 females, aged from 18 to 21 years old, with an average age of 19.3 years old, and the course of disease ranged from 12 to 26 months, with an average of 22 months. All the patients suffered from lumbar pain and difficulty in getting out of bed. Preoperative CT confirmed 12 cases of L5 isthmus fissure and 4 cases of L4 isthmus fissure. Bone graft fusion was confirmed and internal fixation was removed after operation. Lumbar spondylolysis was evaluated by lumbago visual analogue scoring method at preoperative and postoperative time points. Lumbar isthmic fusion was evaluated by lumbar CT, and degeneration of fixed and adjacent segments of lumbar intervertebral disc was evaluated by lumbar MRI. RESULTS: Of the 16 patients, 13 patients (26 sides) were followed up, with a mean duration of 96 months. The operation time ranged from 80 to 105 minutes, with an average of 95 minutes. The intraoperative bleeding volume ranged from 150 to 300 ml, with an average of 225 ml. All the patients were successfully operated without any complications related to the operation. VAS scores at each time point after operation were improved compared with those before operation(P<0.01). Postoperative CT scans of lumbar spine showed osseous fusion at 6 to 14 months, with an average of 12 months. There were no changes of adjacent segment degeneration, fixed segment disc degeneration and protrusion on lumbar spine MRI, and no symptomatic recurrence or recurrent spondylolysis in the long term. CONCLUSIONS: The posterior lumbar pedicle screw fixation combined with isthmic bone grafting and fusion is safe and effective in the treatment of young spondylolysis. The fusion rate is high and the interference of normal physiological range is reduced. The long-term effect is satisfactory.


Assuntos
Parafusos Pediculares , Fusão Vertebral , Espondilólise , Adolescente , Transplante Ósseo , Feminino , Humanos , Vértebras Lombares , Masculino , Estudos Retrospectivos , Espondilólise/cirurgia , Resultado do Tratamento , Adulto Jovem
19.
Huan Jing Ke Xue ; 40(6): 2764-2772, 2019 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854669

RESUMO

Sediments serve as an important carrier during the migration and transformation of pollutants in surface-flow constructed wetlands. Exploring the accumulation characteristics and release patterns of nutrients is of great significance for assessing the purification functions of constructed wetlands after long-term operation. The contents of total organic carbon (TOC), total nitrogen (TN), and total phosphorus (TP) in sediments at various locations of the 5-year-old Yanlong Lake surface-flow constructed wetland were analyzed and static release experiments with cylindrical core samples were carried out. The results showed that after five years, sediments at this site have become moderately polluted and the nutrient accumulation level is mainly being affected by the influent flow, water depth, and plant effects. Additionally, ① nutrients in sediments were found to increase along the flow direction; ② they also increase as the depth of overlying water increases and are relatively affected by the carbon; ③ healthier growth of the wetland plants could lead to more nutrients being accumulated in sediments. The release trend of nutrients fell after a rise and basically reached equilibrium after 20 days, and the release rate was mainly affected by the accumulation level of nutrients, which is similar to the trend in light nutrient containing reservoirs. Plant residues contained in the sediments were associated with the stronger release of nutrients. These results can provide a scientific basis for solutions aimed at maintaining the purification capacity of long-running surface-flow constructed wetlands.

20.
Appl Opt ; 58(31): 8631-8637, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31873354

RESUMO

With fractal amplitude masks of the Sierpinski carpet and Sierpinski triangle, we theoretically and experimentally present the diffraction properties and applications of spatially structured optical fields, including the vector optical field, vortex optical field, and vortex vector optical field. The diffraction patterns of the vector optical fields exhibit self-similarity, and the characteristics of the vector optical fields are maintained in every diffraction peak. The diffraction patterns of the vortex optical fields and vortex vector optical fields exhibit triangular lattice arrays, and the vortex topological charge can be determined by the number of peak spots in the triangular lattice array. We hope these diffraction properties with fractal amplitude masks can be applied not only in detecting topological charges of spatially structured optical fields, but also in generating flexibly controlled diffraction patterns and lattice arrays, which may be useful in optical machining, optical trapping, and information transmission.

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