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1.
Anal Chem ; 2022 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-35968680

RESUMO

Ribose plays an important role in the process of life. Excessive ribose in the human cerebrospinal fluid or urine can be used as an early diagnostic marker of leukoencephalopathy. Fluorinated phenylboronic acid combined with 19F NMR spectroscopy was a powerful method for molecular recognition. However, phenylboronic acid-based sensors for selective detection of ribose are rarely reported in the literature. In this study, the rapid and highly selective recognition of ribose was studied by 19F NMR and 2-fluorophenylboric acid. It was found that 2-fluoro-phenylboric acid was an appropriate 19F NMR-based sensor molecule for the determination of ribose under physiological conditions with high selectivity and robust anti-interference ability. When 2-fluorophenylboric acid was used for the detection of ribose in human urine without any sample pretreatment, a limit of detection of 78 µM was obtained at room temperature under given 19F NMR experimental conditions (400 MHz, 512 scans, ca. 12 min), which can well meet the needs of practical application.

2.
Front Oncol ; 12: 959151, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35936699

RESUMO

Background: Orthotopic liver transplantation (OLT) is a life-saving option for patients with hepatocellular carcinoma (HCC), but the expanded OLT criteria remain controversial. Objective: The study aimed to explore whether expanded OLT criteria can be applied to Chinese cirrhotic patients with HCC. Methods: This retrospective study analyzed risk factors for HCC recurrence and death and compared patients' tumor characteristics and outcomes in groups of Milan, "Up-to-seven," and Hangzhou criteria, and groups between met and unmet the combinative criteria of "Up-to-seven" and AFP of < 1000 ng/mL. Results: Among 153 patients who underwent OLT for HCC from January 2015 to February 2019 in 4 years of follow-up, 20 (13.1%) patients had HCC recurrence, and 11 (7.2%) had HCC-related death. Multivariate Cox regression analysis showed that preoperative alpha-fetoprotein (AFP) of > 1000 ng/mL (hazard ratio [HR]: 10.05, 95% confidence interval [CI]: 2.45-41.13, P = 0.001) was an independent risk factor for HCC recurrence and HCC-related death (HR: 6.63, 95%CI: 1.31-33.52, P = 0.022). Patients who did not meet Milan criteria but satisfied the "Up-to-seven" criteria had no differences in overall survival (OS) (P = 0.69) and disease-free survival (DFS) (P = 0.35) than patients who met the Milan criteria. The combination of "Up-to-seven" criteria and AFP of < 1000 ng/mL differed significantly (HR: 18.9; 95% CI: 4.0-89.2; P < 0.001). Patients with HCC who met the "Up-to-seven" criteria and AFP of < 1000 ng/mL (n = 121) had excellent survival with 4-year OS of 91.6% (P < 0.001) and DFS of 90.8% (P < 0.001), which is significantly better compared to the other group (n = 32) (OS of 67.5% and DFS of 46.5%) and patients who met the Milan criteria (n = 108, OS of 89.8%, DFS of 89.6%), allowing 28.9% (13/45) of patients who did not meet the Milan criteria to benefit from OLT. Conclusion: Chinese cirrhotic patients with HCC who met the combinative criteria of "Up-to-seven" and AFP of < 1000 ng/mL had better survival than those who met the Milan criteria, and these combinative criteria benefited more patients and may become a better option for OLT.

3.
Anal Chem ; 93(19): 7220-7225, 2021 05 18.
Artigo em Inglês | MEDLINE | ID: mdl-33939406

RESUMO

Glucose is a significant analyte both in biology and biomedical science, it is of great importance to selectively detect glucose both in body fluids and complex mixture. In this study, a simple 19F NMR based sensor was synthesized easily, which exhibited a high selectivity and robust anti-interference ability toward glucose detection both in a mixture containing up to 10 saccharides and human urine samples without any pretreatment. Combined with this sensor system, glucose could be well detected in human urine samples and the limit of detection was 0.41 mM by using a 400 MHz NMR spectrometer with 128 scans (ca. 4 min). This method had a potential for specific detection of glucose in complex mixture and diagnosis of diabetes mellitus related diseases in body fluid.


Assuntos
Glucose , Humanos , Espectroscopia de Ressonância Magnética
4.
BMC Cancer ; 21(1): 316, 2021 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-33765954

RESUMO

BACKGROUND: Wilms tumor (WT) is the most common malignant renal tumor in children. The aim of this study was to identify potential susceptibility gene of WT for better prognosis. METHODS: Weighted gene coexpression network analysis is used for the detection of clinically important biomarkers associated with WT. RESULTS: In the study, 59 tissue samples from National Cancer Institute were pretreated for constructing gene co-expression network, while 224 samples also downloaded from National Cancer Institute were used for hub gene validation and module preservation analysis. Three modules were found to be highly correlated with WT, and 44 top hub genes were identified in these key modules eventually. In addition, both the module preservation analysis and gene validation showed ideal results based on other dataset with 224 samples. Meanwhile, Functional enrichment analysis showed that genes in module were enriched to sister chromatid cohesion, cell cycle, oocyte meiosis. CONCLUSION: In summary, we established a gene co-expression network to identify 44 hub genes are closely to recurrence and staging of WT, and 6 of these hub genes was closely related to the poor prognosis of patients. Our findings revealed that those hub genes may be used as potential susceptibility gene for clinical diagnosis and prognosis of this tumor.


Assuntos
Biomarcadores Tumorais/genética , Redes Reguladoras de Genes , Neoplasias Renais/genética , Recidiva Local de Neoplasia/epidemiologia , Tumor de Wilms/genética , Criança , Biologia Computacional , Conjuntos de Dados como Assunto , Perfilação da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Predisposição Genética para Doença , Testes Genéticos/métodos , Humanos , Neoplasias Renais/epidemiologia , Recidiva Local de Neoplasia/genética , Estadiamento de Neoplasias , Prognóstico , Medição de Risco/métodos , Tumor de Wilms/diagnóstico , Tumor de Wilms/epidemiologia
5.
World J Gastroenterol ; 26(35): 5287-5301, 2020 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-32994688

RESUMO

BACKGROUND: Serum amyloid A1 (SAA1) is an acute-phase protein involved in acute or chronic hepatitis. Its function is still controversial. In addition, the effect of the expression of SAA1 and its molecular function on the progression in hepatocellular carcinoma (HCC) is still unclear. AIM: To demonstrate the expression of SAA1 and its effect on the prognosis in HCC and explain further the correlation of SAA1 and immunity pathways. METHODS: SAA1 expression in HCC was conducted with The Cancer Genome Atlas-Liver Hepatocellular Carcinoma (TCGA-LIHC) in GEPIA tool, and the survival analysis based on the SAA1 expression level was achieved in the Kaplan-Meier portal. The high or low expression group was then drawn based on the median level of SAA1 expression. The correlation of SAA1 and the clinical features were conducted in the UALCAN web-based portal with TCGA-LIHC, including tumor grade, patient disease stage, and the TP53 mutation. The correlation analysis between SAA1 expression and TP53 mutation was subjected to the TCGA portal. The tumor purity score and the immune score were analyzed with CIBERSORT. The correlation of SAA1 expression and tumor-infiltrating lymphocytes was achieved in TISIDB web-based integrated repository portal for tumor-immune system interactions. GSE125336 dataset was used to test the SAA1 expression in the responsive or resistant group with anti-PD1 therapy. Gene set enrichment analysis was applied to evaluate the gene enrichment signaling pathway in HCC. The similar genes of SAA1 in HCC were identified in GEPIA, and the protein-protein interaction of SAA1 was conducted in the Metascape tool. The expression of C-X-C motif chemokine ligand 2, C-C motif chemokine ligand 23, and complement C5a receptor 1 was studied and overall survival analysis in HCC was conducted in GEPIA and Kaplan-Meier portal, respectively. RESULTS: SAA1 expression was decreased in HCC, and lower SAA1 expression predicted poorer overall survival, progression-free survival, and disease-specific survival. Furthermore, SAA1 expression was further decreased with increased tumor grade and patient disease stage. Also, SAA1 expression was further downregulated in patients with TP53 mutation compared with patients with wild type TP53. SAA1 expression was negatively correlated with the TP53 mutation. Lower SAA1 predicted poorer survival rate, especially in the patients with no hepatitis virus infection, other than those with hepatitis virus infection. Moreover, the SAA1 expression was negatively correlated with tumor purity. In contrast, SAA1 expression was positively correlated with the immune score in HCC, and the correlation analysis between SAA1 expression and tumor-infiltrating lymphocytes also showed a positive correlation in HCC. Decreased SAA1 was closely associated with the immune tolerance of HCC. C-X-C motif chemokine ligand 2 and C-C motif chemokine ligand 23 genes were identified as the hub genes associated with SAA1, which could also serve as favorable prognosis markers for HCC. CONCLUSION: SAA1 is downregulated in the liver tumor, and it is closely involved in the progression of HCC. Lower SAA1 expression indicates lower survival rate, especially for those patients without hepatitis virus infection. Lower SAA1 expression also suggests lower immune infiltrating cells, especially for those with immune cells exerting anti-tumor immune function. SAA1 expression is closely associated with the anti-tumor immune pathways.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , Biomarcadores Tumorais/genética , Carcinoma Hepatocelular/genética , Humanos , Estimativa de Kaplan-Meier , Neoplasias Hepáticas/genética , Linfócitos do Interstício Tumoral , Prognóstico
6.
Drug Des Devel Ther ; 14: 1779-1798, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32440103

RESUMO

BACKGROUND: Radiotherapy has an ameliorative effect on a wide variety of tumors, but hepatocellular carcinoma (HCC) is insensitive to this treatment. Overactivated mammalian target of rapamycin (mTOR) plays an important part in the resistance of HCC to radiotherapy; thus, mTOR inhibitors have potential as novel radiosensitizers to enhance the efficacy of radiotherapy for HCC. METHODS: A lead compound was found based on pharmacophore modeling and molecular docking, and optimized according to the differences between the ATP-binding pockets of mTOR and PI3K. The radiosensitizing effect of the optimized compound (2a) was confirmed by colony formation assays and DNA double-strand break assays in vitro. The discovery and preclinical characteristics of this compound are described. RESULTS: The key amino acid residues in mTOR were identified, and a precise virtual screening model was constructed. Compound 2a, with a 4,7-dihydro-[1,2,4]triazolo[1,5-a]pyrimidine scaffold, exhibited promising potency against mTOR (mTOR IC50=7.1 nmol/L (nM)) with 126-fold selectivity over PI3Kα. Moreover, 2a significantly enhanced the sensitivity of HCC to radiotherapy in vitro in a dose-dependent manner. CONCLUSION: A new class of selective mTOR inhibitors was developed and their radiosensitization effects were confirmed. This study also provides a basis for developing mTOR-specific inhibitors for use as radiosensitizers for HCC radiotherapy.


Assuntos
Carcinoma Hepatocelular/terapia , Neoplasias Hepáticas/terapia , Pirimidinonas/farmacologia , Radiossensibilizantes/farmacologia , Serina-Treonina Quinases TOR/antagonistas & inibidores , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/patologia , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Avaliação Pré-Clínica de Medicamentos , Humanos , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/patologia , Modelos Moleculares , Estrutura Molecular , Pirimidinonas/síntese química , Pirimidinonas/química , Radiossensibilizantes/síntese química , Radiossensibilizantes/química , Relação Estrutura-Atividade , Serina-Treonina Quinases TOR/metabolismo
7.
Nanotechnology ; 31(14): 145203, 2020 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-31860897

RESUMO

In this paper, we fabricate ordered pore array (OPA) Ag film coated glass with the aid of polystyrene sphere (PS) array templates. This kind of OPA Ag coated glass has optical advantages of visible transparency, blue and near-infrared resistance. The average visible transmittance is 68%, including a transmission peak of 78% located at 570 nm, and low average transmittance of 48% in the blue light region that is not damaging to the eyes. The near-infrared light blocking rate is 67%, among which 40% light is reflected directly, indicating the reflection domination.

8.
Nanoscale Res Lett ; 14(1): 298, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31463645

RESUMO

Eu2+-activated phosphors are widely applied in lighting and display areas because of their good optical performance. In this paper, an excellent green-emitting zeolite-3A: 1.3 wt% Eu phosphor is prepared by a green and eco-friendly high-thermal reaction method without any reducing atmosphere or agents. Meanwhile, the reducing mechanism from Eu3+ ions to Eu2+ ions is investigated. The experiment results show that the morphology, crystal structure, and luminescent property are affected by sintering temperature. The resulting sample shows the broad excitation band is in the range of 310-450 nm and the peak of the broad emission band is located at 523 nm. Furthermore, zeolite-3A: 1.3 wt% Eu phosphor is encapsulated on a commercial UV-emitting chip to fabricate a purity green light-emitting diode (LED) with the Commission Internationale de L'Eclairage (CIE) color coordinates at (0.295, 0.537).

9.
Sci Rep ; 7(1): 144, 2017 03 10.
Artigo em Inglês | MEDLINE | ID: mdl-28273944

RESUMO

Composite hollow nanostructure composed by transition metal oxides are promising materials in electrochemistry, catalyst chemistry and material science. In this contribution, necklace-like NiO-CuO heterogeneous composite hollow nanostructures were synthesized by annealing Ni/Cu superlattice nanowires in air. Two kinds of morphologies including CuO nanotube linked core-shell structures and CuO nanotube linked hollow structures were obtained. The structure can be tuned easily by adjusting the relative length of Cu segments in Ni/Cu superlattice nanowires and the annealing temperature. The relative diffusion amount of Cu to Ni segments was proved to be the key factor to influence the annealed sample morphology. The formation mechanism was discussed in detail based on Kirkendal effect and high temperature oxidation of alloy. We demonstrated that hollow structure or core-shell structure is related to whether the oxidation exists only in external sites or co-exists in external and internal sites during annealing.

10.
Infect Dis Poverty ; 5(1): 101, 2016 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-27806732

RESUMO

BACKGROUND: Clinical and laboratory data were collected and analysed from patients with Ebola virus disease (EVD) in Jui Government Hospital in Freetown, Sierra Leone, where patients with EVD were received and/or treated from October 1, 2014 to March 21, 2015 during the West Africa EVD outbreak. METHODS: The study admitted 285 patients with confirmed EVD and followed them up till the endpoint (recovery or death). EVD was confirmed by quantitative RT-PCR assays detecting blood Ebola virus (EBOV). RESULTS: Among the 285 lab-confirmed EVD cases in Jui Government Hospital, 146 recovered and 139 died, with an overall survival rate of 51.23 %. Patients under the age of 6 years had a lower survival rate (37.50 %). Most non-survivors (79.86 %) died within 7 days after admission and the mean hospitalization time for non-survivors was 5.56 ± 6.11 days. More than half survivors (63.69 %) turned blood EBOV negative within 3 weeks after admission and the mean hospitalization time for survivors was 20.38 ± 7.58 days. High blood viral load (≥106 copies/ml) was found to be predictive of the non-survival outcome as indicated by the Receiver Operating Characteristic (ROC) curve analysis. The probability of patients' survival was less than 15 % when blood viral load was greater than 106 copies/ml. Multivariate analyses showed that blood viral load (P = 0.005), confusion (P = 0.010), abdominal pain (P = 0.003), conjunctivitis (P = 0.035), and vomiting (P = 0.004) were factors independently associated with the outcomes of EVD patients. CONCLUSIONS: Most death occurred within 1 week after admission, and patients at the age of 6 or younger had a lower survival rate. Most surviving patients turned blood EBOV negative within 1-4 weeks after admission. Factors such as high blood viral load, confusion, abdominal pain, vomiting and conjunctivitis were associated with poor prognosis for EVD patients.


Assuntos
Ebolavirus/fisiologia , Doença pelo Vírus Ebola/diagnóstico , Doença pelo Vírus Ebola/mortalidade , Hospitalização , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Doença pelo Vírus Ebola/fisiopatologia , Doença pelo Vírus Ebola/prevenção & controle , Hospitalização/estatística & dados numéricos , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Retrospectivos , Serra Leoa , Carga Viral , Adulto Jovem
11.
Phys Chem Chem Phys ; 18(48): 32691-32696, 2016 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-27711600

RESUMO

Tellurium nanowires (NWs) are attractive one-dimensional materials for many applications, yet most synthesis processes require hazardous chemical reducing agents and extreme operating conditions. Here we described a solvothermal synthesis of Te NWs using a non-toxic reducing agent, ascorbic acid. Then the Te NWs were assembled into a well-aligned film through a stirring-assisted oil-water-air interface assembly method and a Te NWs photodetector was fabricated which is sensitive to infrared radiation. The photodetector based on the well-aligned Te NWs film had a series of more excellent photoelectric properties than that based on those being randomly oriented. For example, the photoresponsivity of the former is 103 times larger, and the response time is 1.15 × 103 times shorter, than those of the latter.

12.
Nanotechnology ; 27(37): 375601, 2016 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-27487089

RESUMO

One-dimensional noble-metal Ag nanoparticle chains have been prepared by electrodepositing Ag/Bi superlattice nanowires in a porous anodic alumina oxide (AAO) template and following an annealing process in vacuum. It is found that Bi, as a sacrificial metal, can be removed completely after annealing at 450 °C with a vacuum degree of 10(-5) Torr. The regulation of particle size, shape and interparticle spacing of Ag NP chains has been realized by adjusting the segment length of the Ag/Bi superlattice nanowires and the annealing condition. With an extension of the annealing time, it is observed that Ag particles display the transform trend from ellipsoid to sphere. Our findings could inspire further investigation on the design and fabrication of metal nanoparticle chains.

13.
Oncotarget ; 7(19): 27910-5, 2016 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-27058894

RESUMO

During the 2014 Ebola virus disease (EVD) outbreak, less than half of EVD-suspected cases were laboratory tested as Ebola virus (EBOV)-negative, but disease identity remained unknown. In this study we investigated the etiology of EVD-like illnesses in EBOV-negative cases. From November 13, 2014 to March 16, 2015, EVD-suspected patients were admitted to Jui Government Hospital and assessed for EBOV infection by real-time PCR. Of 278 EBOV negative patients, 223 (80.21%), 142 (51.08%), 123 (44.24%), 114 (41.01%), 59 (21.22%), 35 (12.59%), and 12 (4.32%) reported fever, headache, joint pain, fatigue, nausea/vomiting, diarrhea, hemorrhage, respectively. Furthermore, 121 (43.52%), 44 (15.83%), 36 (12.95%), 33 (11.87%), 23 (8.27%), 10 (3.60%) patients were diagnosed as infection with malaria, HIV, Lassa fever, tuberculosis, yellow fever, and pneumonia, respectively. No significant differences in clinical features and symptoms were found between non-EVD and EVD patients. To the best of our knowledge, the present study is the first to explore the etiology of EVD-like illnesses in uninfected patients in Sierra Leone, highlighting the importance of accurate diagnosis to EVD confirmation.


Assuntos
Surtos de Doenças , Ebolavirus/isolamento & purificação , Febre/diagnóstico , Febre/etiologia , Hemorragia/etiologia , Doença pelo Vírus Ebola/diagnóstico , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Artralgia/etiologia , Criança , Pré-Escolar , Diarreia/etiologia , Fadiga/etiologia , Feminino , HIV/isolamento & purificação , Cefaleia/etiologia , Doença pelo Vírus Ebola/epidemiologia , Doença pelo Vírus Ebola/virologia , Humanos , Vírus Lassa/isolamento & purificação , Masculino , Pessoa de Meia-Idade , Mycobacterium tuberculosis/isolamento & purificação , Náusea/etiologia , Plasmodium malariae/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real , Serra Leoa/epidemiologia , Vômito/etiologia , Vírus da Febre Amarela/isolamento & purificação , Adulto Jovem
14.
Int J Infect Dis ; 42: 34-39, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26523640

RESUMO

BACKGROUND: A Chinese medical team managed Ebola virus disease (EVD) patients in Sierra Leone from October 2014 to March 2015 and attended to 693 suspected patients, of whom 288 had confirmed disease. METHODS: A retrospective study was conducted of the 288 patients with confirmed disease. Clinical symptoms, manifestations, and serum viral load were analyzed and compared among the different groups for mortality and survival time. RESULTS: Among the 288 confirmed EVD patients (149 male and 139 female, median age 28 years, and median log viral load 6.68), 98 died, 36 recovered, and 154 were lost to follow-up. Common symptoms were fever (77.78%), fatigue (64.93%), abdominal pain (64.58%), headache (62.85%), and diarrhea (61.81%). Compared to patients aged<18 years, those who were older than 40 years had a higher probability of death (odds ratio 2.855, p=0.044). Patients with a viral load of >10(6) copies/ml had a higher case fatality rate than those with <10(6) copies/ml (odds ratio 3.095, p=0.004). Cox regression showed that age, viral load, and the presence of diarrhea correlated with mortality. CONCLUSION: Patients with a high viral load, of older age, and with diarrhea had a higher mortality and shorter survival time.


Assuntos
Doença pelo Vírus Ebola/mortalidade , Carga Viral , Adulto , Fatores Etários , Idoso , Diarreia/virologia , Ebolavirus/isolamento & purificação , Feminino , Doença pelo Vírus Ebola/virologia , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos
15.
Parasitol Res ; 114(9): 3509-19, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26122990

RESUMO

Schistosomiasis is the second widespread tropical disease that affects the health of over 240 million people of 78 countries. Questionnaires have been commonly used to diagnose schistosomiasis, while no meta-analysis of their efficacy had been reported previously. This meta-analysis was conducted to assess their diagnostic accuracy of schistosomiasis. Studies published prior to December 1, 2014, that had used questionnaires as a diagnostic tool were searched in PubMed, Medline, EMBASE, and China National Knowledge Infrastructure (CNKI) database. A total of 32 studies with 72,812 cases were identified for the meta-analysis. The best diagnostic odds ratio (DOR) was obtained from Schistosoma haematobium (67.68, 95 % confidence interval (CI) = 31.48 to 145.54), followed by Schistosoma japonicum (11.74, 95 % CI = 4.59 to 30.05) then Schistosoma mansoni (2.98, 95 % CI = 1.95 to 4.54). Pooled sensitivity and specificity were respectively 0.82, 0.88, and 0.46, and 0.59, 0.86, and 0.81 for S. japonicum, S. haematobium, and S. mansoni. The multivariable subgroup analyses showed that the questionnaires which had been pretested and standardized had better diagnostic performance. The result suggested that questionnaires can be used to diagnose schistosomiasis with moderate sensitivity and specificity and the questionnaires for diagnosing S. haematobium performed best.


Assuntos
Schistosoma/fisiologia , Esquistossomose/diagnóstico , Animais , Humanos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Inquéritos e Questionários/normas
16.
Artigo em Chinês | MEDLINE | ID: mdl-26930945

RESUMO

OBJECTIVE: To assess the impact of the "Oriental Star shipwreck event" on the risk of schistosomiasis transmission in Jianli County, Hubei Province, so as to provide the evidence for formulating appropriate measures of schistosomiasis prevention and control. METHODS: Based on the field observation and investigation, the data of the on-the-spot rescue and the historical endemic situation of schistosomiasis in Jianli County were collected and analyzed. Meanwhile, the focus group discussion and risk matrix were conducted to assess the risk of schistosome infection of the rescuers. RESULTS: Over 10 000 rescuers participated in the search operation, including the armed police forces, local people, reservists and so on. The armed police forces were the major components, which accounted for 39%. Jianli country was schistosomiasis endemic area with a high infection level in history, but the endemic situation had been mitigated significantly after years of positive prevention and treatment; the schistosome infection rate of population was 0.44% in 2014 and the Oncomelania hupensis snail area was only 6.6 hm2 around the rescue areas in the upstream and downstream. In addition, the snail density was not high, and no infected snails had been found for 11 years. The risk of schistosome infection was in the medium level. CONCLUSIONS: Though there exists the schistosome infection risk in the water area where the accident happened, the probability of occurrence is not high. In order to prevent the outbreak and endemic of schistosomiasis, the protection measures and health education to the rescuers as well as snail control and monitoring, and early-warning should be strengthened.


Assuntos
Surtos de Doenças/prevenção & controle , Trabalho de Resgate , Medição de Risco/métodos , Esquistossomose/transmissão , Navios , Acidentes , Animais , China , Erradicação de Doenças/métodos , Erradicação de Doenças/estatística & dados numéricos , Reservatórios de Doenças/parasitologia , Água Doce/parasitologia , Geografia , Educação em Saúde/métodos , Humanos , Medição de Risco/estatística & dados numéricos , Fatores de Risco , Rios/parasitologia , Schistosoma/fisiologia , Esquistossomose/parasitologia , Caramujos/parasitologia
17.
Medicine (Baltimore) ; 93(28): e194, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25526436

RESUMO

Hepatic angiomyolipoma (HAML) is a rare mesenchymal tumor of the liver with marked histological diversity. The present study was to review the magnetic resonance imaging (MRI) and clinical pathological features of HAML resembling hepatocellular carcinoma (HCC). Nine patients who underwent surgical resection and had pathological diagnosis of HAML were retrospectively analyzed. All of 9 patients (5 males and 4 females) had a solitary hepatic mass with a median size of 4 cm (from 1.4 cm to 15.3 cm). Seven cases were identified as incidental liver tumors during health screening and 2 patients were diagnosed for hepatic mass when visited hospitals with unspecific abdominal discomfort. Before resection, 6 cases were diagnosed as HCC on MRI. MRI on chemical shift imagings showed a large amount of lipids in 5 cases. The enhancement pattern of MRI was classified into 2 types: in 2 cases, lesions with small or no vessels that demonstrated prolonged enhancement (1 mixed subtype and 1 myomatous subtype) and in 7 cases, lesions with abundant central vessels that show rapid washout (3 mixed subtypes and 4 myomatous subtypes) in the portal venous/delayed phase. All patients underwent resection of hepatic tumor and no recurrence was observed during follow-up (range: 2-24 months) of median 10 months. By immunohistochemistry, the tumor cells demonstrated positive immunostaining for human melanoma black-45, smooth muscle actin, and CD34. In conclusion, all of 9 patients with HAML presented with none or nonspecific clinical manifestations. The diagnosis of HAML relies on disease and immunohistochemistry, but not MRI due to its resemblance to HCC.


Assuntos
Angiomiolipoma/diagnóstico , Carcinoma Hepatocelular/diagnóstico , Neoplasias Hepáticas/diagnóstico , Fígado/patologia , Imageamento por Ressonância Magnética/métodos , Adulto , Angiomiolipoma/cirurgia , Diagnóstico Diferencial , Feminino , Seguimentos , Hepatectomia , Humanos , Fígado/cirurgia , Neoplasias Hepáticas/cirurgia , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Retrospectivos , Fatores de Tempo
18.
Chem Commun (Camb) ; 50(92): 14317-20, 2014 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-24955939

RESUMO

This communication reports an approach to fabricate large-scale ultrathin open-ended porous TiO2 membranes (UOP-TMs) with ordered straight-through pores. Bi nanodot arrays on Si substrates are obtained by using the UOP-TMs as surface patterning masks.

19.
Liver Int ; 34(1): 136-46, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23875825

RESUMO

BACKGROUND: Epigenetic alterations are well documented in hepatocarcinogenesis. However, hypomethylation of long interspersed nuclear element 1(LINE-1) promoter and its relationship with clinicopathological features in hepatocellular carcinoma(HCC) remain unknown. METHODS: The bisulfite-specific PCR and DNA sequencing analysis was performed to assess the methylation status of LINE-1 promoter in a pilot cohort of 71 patients with HCC. Additionally,methylation levels of two hot CpG sites of LINE-1 promoter, site 7 and 18 were measured by real-time PCR and compared with clinicopathological parameters in a cohort of 172 HCC. All the patients included were in BCLC stage A or B. RESULTS: Most patients with HCC (87.3%) showed hypomethylation of LINE-1 promoter compared with HBV-related cirrhosis and normal controls (P < 0.001). The HCC patients with LINE-1 promoter hypomethylation had a median tumour-free survival (TFS) and overall survival (OS)post-resection of 22.0 (95% CI: 13.3­30.7) months and 35.0 (95% CI: 24.0­46.1) months, respectively, compared with 40 months and ~60 months for those with LINE-1 promoter hypermethylation (P < 0.05). Multivariate analyses showed that the hypomethylation level at CpG site 7 and 18 of LINE-1 promoter, along with tumour size and tumour differentiation, was independently associated with both TFS and OS for patients with HCC after resection. CONCLUSION: Promoter hypomethylation of LINE-1, especially at the CpG site 7 and 18, was associated with a poor prognosis in HCC.


Assuntos
Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/cirurgia , Metilação de DNA , Hepatectomia , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/cirurgia , Elementos Nucleotídeos Longos e Dispersos/genética , Regiões Promotoras Genéticas , Adolescente , Adulto , Idoso , Sequência de Bases , Carcinoma Hepatocelular/mortalidade , Carcinoma Hepatocelular/patologia , Diferenciação Celular , Distribuição de Qui-Quadrado , Ilhas de CpG , Intervalo Livre de Doença , Epigênese Genética , Feminino , Hepatectomia/efeitos adversos , Hepatectomia/mortalidade , Humanos , Neoplasias Hepáticas/mortalidade , Neoplasias Hepáticas/patologia , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Análise Multivariada , Modelos de Riscos Proporcionais , Estudos Prospectivos , Reação em Cadeia da Polimerase em Tempo Real , Fatores de Risco , Análise de Sequência de DNA , Fatores de Tempo , Resultado do Tratamento , Carga Tumoral , Adulto Jovem
20.
Zhonghua Yi Xue Za Zhi ; 93(31): 2505-7, 2013 Aug 20.
Artigo em Chinês | MEDLINE | ID: mdl-24300277

RESUMO

OBJECTIVE: To evaluate the diagnostic significance of Fascin protein in non-small cell lung cancer (NSCLC) patients. METHODS: Among 110 cases of NSCLC patients and 50 cases of healthy controls, double antibody sandwich enzyme-linked immunosorbent assay (ELISA) was employed to measure the serum levels of Fascin protein, carcinoembryonic antigen (CEA), neuron-specific enolase (NSE), scales like squamous cell carcinoma (SCC) antigen, cytokeratin protein 21-1 (CYFRA21-1) and gastrin-releasing peptide (GRP) precursor. Fascin-positive rate (24.4%) in NSCLC patients was significantly higher than that in healthy controls (4%) (P < 0.05).In different clinical stages of non-small cell lung cancer patients, Fascin-positive rate was statistically significant (P < 0.05). The positive rates of Fascin for CEA, CYFRA21-1, NSE, SCC and Pro-GRP were 25.64%, 5.1%, 5.1%, 5.0% and 1.7% respectively. CONCLUSION: As a new tumor marker, Fascin may be used for clinical screening and prognosis in lung cancer.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/diagnóstico , Proteínas de Transporte/sangue , Neoplasias Pulmonares/diagnóstico , Proteínas dos Microfilamentos/sangue , Adulto , Idoso , Biomarcadores Tumorais/sangue , Carcinoma Pulmonar de Células não Pequenas/sangue , Estudos de Casos e Controles , Feminino , Humanos , Neoplasias Pulmonares/sangue , Masculino , Pessoa de Meia-Idade
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