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1.
Food Chem ; 367: 130726, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34352698

RESUMO

The impact of different complexes on the properties of ß-carotene-loaded emulsions was investigated by measuring the droplet size, encapsulation efficiency, droplet morphology, and physical stability. The photo and thermal stability of ß-carotene and its bioaccessibility during digestion were also analyzed. Comparing to the emulsions stabilized by other complexes, the emulsion stabilized by the high methoxyl pectin-rhamnolipid-pea protein isolate-curcumin (HMP-Rha-PPI-Cur) complex had the smallest droplet size (17.53 ± 0.15 µm) and the maximum encapsulation efficiency for curcumin (90.33 ± 0.03 %) and ß-carotene (92.16 ± 0.01 %). The emulsion stabilized by the HMP-Rha-PPI-Cur complex exhibited better physical stability against creaming. The retention rate of ß-carotene in the HMP-Rha-PPI-Cur complex-stabilized emulsion was 17.75 ± 0.02 and 33.64 ± 0.02 % after UV irradiation and thermal treatment. The HMP-Rha-PPI-Cur complex-stabilized emulsion also had a higher level of free fatty acid released (43.67 %) and higher bioaccessibility of ß-carotene (32.35 ± 0.02 %).


Assuntos
Curcumina , Proteínas de Ervilha , Carotenoides , Emulsões , Tamanho da Partícula , Pectinas , Tensoativos , beta Caroteno
2.
Food Chem ; 367: 130762, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34390912

RESUMO

Inhibitory effects of flavonoids on starch digestibility were well known, but the structural mechanism was not clear. This study was focused on the diverse effect of quercetin and rutin on digestibility of Tartary buckwheat starch. Results showed that quercetin and rutin reduced the starch digestion by altering starch structure in bound forms and inhibiting digestive enzyme activity in free forms simultaneously, and quercetin showed a stronger effect than rutin. Molecular docking and saturation transfer difference-nuclear magnetic resonance (STD-NMR) revealed different binding site of rutin from quercetin was due to its hydroxyl and hydrogen on the glycoside structure. Rutin interacted with enzymes mainly by CH and OH on the glycoside structure which induced steric hindrance and restricted the inhibitory effect of quercetin fraction. The glycoside structure weakened inhibition of rutin on digestive enzymes in free forms rather than influence its anti-digestive effects in bound forms with starch.


Assuntos
Fagopyrum , Rutina , Sítios de Ligação , Digestão , Simulação de Acoplamento Molecular , Quercetina , Amido
3.
Food Chem ; 373(Pt A): 131414, 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34717089

RESUMO

In this study, two modified silica nanoparticles (SiO2-GA NPs) were successfully obtained by covalently grafting gallic acid onto silica nanoparticles. The mean particle diameters of their were 112.7 ± 0.55 nm (1-SiO2-GA NPs) and 408.7 ± 3.20 nm (4-SiO2-GA NPs), respectively. Novel antioxidant active packaging composite films were prepared by incorporation of 1-SiO2-GA NPs or 4-SiO2-GA NPs into chitosan. The structure analysis of the composite films showed that intermolecular hydrogen bonds were formed between the two modified silica nanoparticles and chitosan. Compared with the chitosan film, the mechanical properties, water vapor barrier property and UV light barrier ability of the composite films were significantly improved. Moreover, the incorporated of the two modified silica nanoparticles significantly increased antioxidant activity of the composite films. This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.

4.
Food Funct ; 12(21): 10842-10861, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34617943

RESUMO

In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of ß-carotene. The photothermal stability and storage stability of ß-carotene were improved with the combined use of different particles. Microstructural observations showed that ZPCPs were effectively adsorbed at the oil/water interface despite the substantial interparticle gaps. WPM particles could swell and stretch on the interface due to their deformable structure, thereby forming an interfacial layer of flattened particles to cover a large surface area. The interfacial structure and macroscopic properties of Pickering emulsions were modulated by adjusting the mass ratio and addition sequence of different particles. The combination of ZPCPs and WPM delayed the lipolysis during gastrointestinal digestion. Through controlling the composition of the complex interface, the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase was reduced from 15.64% to 9.03%. When ZPCPs were used as the inner layer and WPM as the outer layer and the mass ratio of ZPCPs to WPM was 4 : 1, the Pickering emulsion showed the best stability and ß-carotene bioaccessibility. The Pickering emulsion with particle-particle complex interfaces could be applied in foods and pharmaceuticals for the purpose of enhanced stability, delayed lipolysis or sustained nutrient release.

5.
J Agric Food Chem ; 69(41): 12278-12294, 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34530616

RESUMO

In this study, we utilized different types of particles to stabilize ß-carotene-loaded Pickering emulsions: spherical hydrophobic zein colloidal particles (ZCPs) (517.3 nm) and rod-shaped hydrophilic cellulose nanocrystals (CNCs) (115.2 nm). Either of the particles was incapable of stabilizing Pickering emulsions owing to their inappropriate wettability. When the mass ratio of ZCPs and CNCs was 1:4, the Pickering emulsion showed the best physical and photothermal stability. Compared to the ZCP-stabilized Pickering emulsion (9.29%), the retention rate of ß-carotene in the Pickering emulsion costabilized by ZCPs and CNCs was increased to 60.23% after 28 days of storage at 55 °C. Confocal microscopy and cryoscanning electron microscopy confirmed that different types of particles could form a multilayered structure or induce the formation of an interparticle network. Furthermore, the complexation of ZCPs and CNCs delayed the lipolysis of the emulsion during in vitro digestion. The free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase was reduced from 19.46 to 8.73%. Accordingly, the bioaccessibility of ß-carotene in Pickering emulsions ranged from 9.14 to 27.25% through adjusting the mass ratio and addition sequence of distinct particles at the interface. The Pickering emulsion with the novel particle-particle complex interface was designed in foods and pharmaceuticals for purpose of enhanced stability, delayed lipolysis, or sustained nutrient release.


Assuntos
Nanopartículas , Zeína , Celulose , Emulsões , Tamanho da Partícula , beta Caroteno
6.
Front Physiol ; 12: 696286, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34305650

RESUMO

Developing a three-dimensional (3D) visualization of the kidney at the whole-mount scale is challenging. In the present study, we optimized mouse whole-mount kidney clearing, which improved the transparency ratio to over 90% based on organ-specific perfusion (OSP)-clear, unobstructed brain imaging cocktails and computational analysis (CUBIC). The optimized OSP-CUBIC-compatible 3D immunostaining and imaging simultaneously visualized the high-resolution 3D structure of the whole-mount renal microvascular, glomerulus, and accompanying wrapped traveling sympathetic nerves in mice. A mouse model of pressure overload-induced heart failure (HF) was then established by minimally invasive transverse aortic constriction (MTAC). Further 3D quantification revealed renal sympathetic hyperinnervation (6.80 ± 1.04% vs. 3.73 ± 0.60%, P < 0.05) in mice with HF. In conclusion, this newly developed whole-organ tissue clearing and imaging system provides comprehensive information at the whole-mount scale and has great potential for kidney research. Our data suggest that renal sympathetic hyperinnervation is involved in HF associated with renal dysfunction.

7.
Food Funct ; 12(15): 6936-6949, 2021 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-34132729

RESUMO

Novel multilayered core-shell microparticles were developed to deliver curcumin using positively charged zein microparticles coated with negatively charged cellulose nanocrystals (CNCs) and positively charged whey protein microgels (WPMs) at pH 4. Different levels of WPMs (0.10%-1.50%, w/v) were utilized to regulate the structure, stability, and in vitro digestion of curcumin loaded zein-CNC core-shell microparticles. The size of zein-CNC-WPM core-shell microparticles ranged from 2087.7 to 2928.2 nm. The electrostatic attraction and hydrogen bonding were mainly involved in the assembly of the core-shell microparticles through particle-particle interactions. The microstructure of the core-shell microparticles was dependent on the level of the WPM. When its appropriate level was adopted (0.50%-1.00%, w/v), the WPM formed a protective shell for zein-CNC-WPM core-shell microparticles. The retention rate of curcumin in the core-shell microparticles increased by 47.56% and 32.79% during light and thermal treatment, respectively. Excess microgels facilitated the bridging aggregation and formation of a network structure on the particle surface, which further reduced their stability and greatly restricted the curcumin release. The potential of nanosized protein microgels was explored to stabilize and modulate the physicochemical properties of multilayered core-shell microparticles through interparticle interactions.

8.
Food Funct ; 12(7): 3246-3265, 2021 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-33877248

RESUMO

In this study, ß-carotene loaded oil-in-water emulsions were stabilized by complex interfaces composed of propylene glycol alginate (PGA), rhamnolipids (Rha), and zein colloidal particles (ZCPs). The influence of mixed biopolymer-surfactant, biopolymer-particle, surfactant-particle and biopolymer-surfactant-particle interfaces on the performance of the emulsions was investigated. The stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of the emulsions were controlled by regulating the adding sequence and mass ratio of the multiple stabilizers. The droplet size of the emulsion was in the range of 14-77 µm. After encapsulation into the emulsions stabilized by the complex interfaces, the photothermal stability of ß-carotene were increased by 41.53% and 21.52%, respectively. The co-existence of particles, biopolymers, and surfactants could induce competitive displacement, multilayer deposition and an interparticle network at the interface. Compared with a single PGA- or Rha-stabilized emulsion, the complex interface-stabilized emulsion reduced the release of FFA by 28.06% and 26.16%, respectively. The interfacial composition of the emulsion and the delayed lipid digestion further affected the bioaccessibility of ß-carotene in the gastrointestinal tract (GIT). The mixed biopolymer-particle-surfactant interface-stabilized emulsion could be incorporated in foods, pharmaceuticals and cosmetics for excellent stability, targeted nutrient delivery and controlled lipolysis.


Assuntos
Biopolímeros/química , Emulsões/química , Tensoativos/química , beta Caroteno/química , beta Caroteno/farmacocinética , Disponibilidade Biológica , Digestão , Estabilidade de Medicamentos , Elasticidade , Emulsões/metabolismo , Trato Gastrointestinal/metabolismo , Microscopia Eletrônica de Varredura , Tamanho da Partícula , Pepsina A/metabolismo , Viscosidade , Zeína/química , beta Caroteno/administração & dosagem
9.
Food Chem ; 357: 129849, 2021 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-33915467

RESUMO

The novel core-shell microparticles were fabricated to deliver curcumin by using hydrophobic zein microparticles as the core and hydrophilic cellulose nanocrystals (CNCs) as the shell. Different concentrations (0.10-1.50%, w/v) of CNCs were utilized to regulate the microstructure, physicochemical stability, and in vitro digestion of the core-shell microparticles. The size of the microparticles ranged from 1017.3 to 3663.7 nm. Electrostatic attraction and hydrophobic interactions were responsible for the assembly of zein-CNCs core-shell microparticles. The microstructure of the microparticles was dependent on the CNCs level. The retention rate of curcumin in the core-shell microparticles was increased by 76.41% after UV radiation. Furthermore, the rise of CNCs level delayed the release of curcumin from the microparticles in gastrointestinal tract and reduced its bioaccessibility. The potential of utilizing hydrophilic nanoparticles was explored to stabilize hydrophobic microparticles through interparticle interactions, which was useful to develop the novel core-shell microparticles for the application in functional foods.

10.
Food Chem ; 347: 128978, 2021 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-33444890

RESUMO

A safe and biodegradable γ-cyclodextrin-metal-organic-frameworks (γ-CD-MOFs) was successfully synthesized by using an improved hydrothermal method. In this study, curcumin (Cur) was chosen for testing the encapsulation stability and release performance of γ-CD-MOFs. Results of the crystal structure measurement indicated that the encapsulated curcumin within γ-CD-MOFs via van der Waals forces, hydrophobic interactions and hydrogen bonding was failed to disturb the inherent microtopography and crystallinity of γ-CD-MOFs. Compared to individual γ-CD, the γ-CD-MOFs exhibited improved loading capacity, physicochemical stability as well as controlled-release property in simulated digestion, and hence can be regarded as effective carriers for curcumin. Curcumin-loaded γ-CD-MOFs with a Cur : γ-CD-MOFs mass ratio of 2:3 (Cur-CD-MOFs/3), which showed the highest encapsulation efficiency (67.31 ± 2.25%), improved physicochemical stability and controlled-release performance, was selected for further research and industrialization. Our results demonstrate that γ-CD-MOFs can be regarded as a promising novel carrier for the delivery of curcumin or other hydrophobic nutraceuticals.


Assuntos
Curcumina/química , Portadores de Fármacos/química , Estruturas Metalorgânicas/química , gama-Ciclodextrinas/química , Curcumina/metabolismo , Liberação Controlada de Fármacos , Estabilidade de Medicamentos , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Temperatura
11.
J Agric Food Chem ; 69(5): 1619-1636, 2021 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-33512160

RESUMO

Novel Pickering emulsions were stabilized by complex interfaces in the presence of zein colloidal particles (ZCPs), propylene glycol alginate (PGA), and rhamnolipid (Rha) for delivery of ß-carotene. The influence of the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces on the physiochemical properties and digestion fate of Pickering emulsions was investigated. It is the first time that three different types of emulsifiers have been used to synergistically stabilize food Pickering emulsions for delivery of lipophilic nutraceuticals. The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions. After encapsulation into Pickering emulsions, the retention rate of ß-carotene increased 2-fold under UV radiation for 8 h. The coexistence of ZCPs, PGA, and Rha could induce the competitive displacement, multilayer deposition, and interparticle network at the interface. The combination of particles, a biopolymer, and a surfactant delayed the lipolysis during in vitro gastrointestinal tract. By modulating the interfacial composition, the release rate of free fatty acids from Pickering emulsions was reduced from 19.46% to 2.83% through different mechanisms. The novel Pickering emulsion could be incorporated in foods as well as pharmaceuticals for controlled lipid digestion or targeted nutrient delivery purposes.


Assuntos
Biopolímeros/química , Biopolímeros/metabolismo , Trato Gastrointestinal/metabolismo , Tensoativos/química , beta Caroteno/química , beta Caroteno/metabolismo , Alginatos/química , Digestão , Composição de Medicamentos , Estabilidade de Medicamentos , Emulsões/química , Emulsões/metabolismo , Humanos , Tamanho da Partícula , Reologia , Zeína/química
12.
Food Funct ; 12(3): 1192-1206, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33432315

RESUMO

Microfluidization (50-150 MPa) and thermal treatment (45-85 °C) were applied to modulate the physicochemical stability, molecular interaction and microstructure of zein-proplyene glycol alginate (PGA)-tea saponin (TS) complex nanoparticles for delivery of curcumin. The size of these complex nanoparticles was decreased from 583.1 to 267.4 nm as the microfluidization pressure was increased from 0 to 100 MPa. In the combined treatment of microfluidization and heating, 100 MPa and 75 °C were the optimum parameters to prepare zein-PGA-TS complex nanoparticles for a better protection of curcumin against various environmental stresses. SEM revealed a synergistic effect of microfluidization and heating on the fabrication of complex nanoparticles with a more uniform size and spherical shape. During in vitro gastrointestinal digestion, the complex nanoparticles showed an excellent gastric stability and a sustained release of curcumin in the small intestinal phase. These findings interpreted the effects of microfluidization and thermal treatment on the functional properties of protein-polysaccharide-surfactant complex nanoparticles that can be utilized to develop food grade nanoparticles with enhanced stability and controllable digestion behaviour.


Assuntos
Curcumina/química , Nanopartículas/química , Propilenoglicol/química , Saponinas/química , Chá/química , Zeína/química , Dicroísmo Circular , Digestão , Fluorescência , Temperatura Alta , Microfluídica
13.
Food Funct ; 12(1): 70-82, 2021 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-33191429

RESUMO

Synergistic biological activities of probiotics and curcumin can be achieved based on the gut-brain axis. However, it is still a challenge for utilizing both of them in actual food products due to their high sensitivity to environmental conditions. In the present study, high-internal-phase emulsions (HIPEs) were fabricated to co-encapsulate the probiotics and curcumin in response to the customer demand for convenience. ß-Lactoglobulin-propylene glycol alginate composite hydrogel particles (ß-lgPPs) with proper size and intermediate wettability were prepared at ß-lg to PGA mass ratio of 2 : 1 and employed as particulate emulsifiers. Stable HIPEs with a fixed oil fraction (φ = 0.8) could be formed within a wide range of ß-lgPPs concentrations, ranging from 0.1 to 2.0 wt%. Confocal laser scanning microscopy (CLSM) images indicated that the interfacial structure of the oil droplets was composed of both ß-lg nanoparticles and a PGA network, which jointly contributed to the gel-like structures in HIPEs. An increase in elasticity and gel strength, as well as centrifugal stability, could be achieved by elevating the particle concentration as determined by diffusing wave spectroscopy and Lumisizer analysis. HIPEs with high particle concentrations showed a high resistance against pasteurization since no obvious flocculation or coalescence could be observed in these emulsions. HIPEs also provoked a significant reduction in the death of LGG as well as the chemical degradation of curcumin: up to 7.91 log CFU cm-3 of LGG and 93.0% of curcumin were retained after pasteurization treatment. Moreover, the HIPEs could also retard the release of curcumin and protect the LGG in simulated gastrointestinal tract conditions. The results from this work provide useful information for developing a promising delivery system for the co-encapsulation of curcumin and probiotics.


Assuntos
Curcumina/química , Emulsificantes/química , Probióticos/química , Emulsões/química
14.
Food Chem ; 335: 127556, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-32738529

RESUMO

We investigated the interactions of two main phenolics, rutin and quercetin, with starch, the primary component of Tartary buckwheat. The addition of rutin or quercetin significantly affected the structural and physicochemical properties of the starch, and rutin showed a stronger effect than quercetin, particularly at a dose of 6% (w/w). Rutin better enhanced the aggregation of starch pastes and gel formation than quercetin according to our pasting, rheological and thermal property analyses. A scanning electron microscopy analysis of its morphology showed that rutin was more easily dispersed in starchy matrix than quercetin and acted as rigid fillers for gels. The nuclear magnetic resonance results showed different binding sites due to the steric hindrance of the rutin disaccharide groups (rutinose). These findings provide fundamental information about applying rutin during the whole grain processing of Tartary buckwheat.


Assuntos
Fagopyrum/química , Quercetina/química , Reologia , Rutina/química , Amido/química
15.
Food Chem ; 337: 128019, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32927227

RESUMO

In this study, the nanocomplexes as a novel delivery system for curcumin, were successfully fabricated using high methoxyl pectin (HMP), individual surfactants (rhamnolipid (Rha), tea saponin (TS) and ethyl lauroyl arginate hydrochloride (ELA)) and pea protein isolate (PPI). The optimum mass ratio between PPI and curcumin was 40:1. The HMP-Rha-PPI-Cur, HMP-TS-PPI-Cur and HMP-ELA-PPI-Cur complexes which had particle sizes of 453, 422 and 587 nm, exhibited encapsulation efficiencies of curcumin with 93.46, 92.05 and 86.73%, respectively. The analysis of FTIR revealed that HMP-surfactant-PPI-Cur complexes were formed mainly by hydrogen bonding and electrostatic attraction. XRD result showed that curcumin exhibited a non-crystallized state in the ternary complexes. Moreover, the curcumin within the HMP-Rha-PPI ternary complexes showed better stability under UV-light, thermal and simulated gastrointestinal conditions.


Assuntos
Curcumina/administração & dosagem , Proteínas de Ervilha/química , Polissacarídeos/química , Tensoativos/química , Curcumina/química , Glicolipídeos , Ligação de Hidrogênio , Modelos Biológicos , Nanopartículas/química , Tamanho da Partícula , Pectinas/química
16.
Carbohydr Polym ; 254: 117446, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33357916

RESUMO

Probiotics and curcumin can exhibit synergistic biological activities on the basis of a gut-brain axis, but are sensitive to environmental conditions, making it a challenge for their co-utilization. To meet the demand for high efficiency and convenience, both probiotics and curcumin were encapsulated within a propylene glycol alginate-based hydrogel delivery system, which was assembled using an ethanol-induced approach. The composite hydrogel was effective at sustaining the release of curcumin and protecting LGG cells in simulated gastrointestinal tract conditions. Moreover, it could also largely reduce the chemical degradation of curcumin and increase the survival of LGG during light exposure and long-term storage: up to 91.3 % of curcumin and 9.72 log CFU cm-3 remained present throughout 4 weeks of storage. Results in this work demonstrate a low-energy and green approach to assemble a composite hydrogel with remarkable biocompatibility, which is considered as a desired delivery vehicle for co-delivery of probiotics and curcumin.


Assuntos
Alginatos/química , Curcumina/metabolismo , Preparações de Ação Retardada , Lactobacillus rhamnosus/fisiologia , Lactoglobulinas/química , Probióticos/análise , Materiais Biomiméticos/química , Encapsulamento de Células/métodos , Curcumina/química , Composição de Medicamentos/métodos , Liberação Controlada de Fármacos , Estabilidade de Medicamentos , Etanol/química , Suco Gástrico/química , Humanos , Hidrogéis , Concentração de Íons de Hidrogênio , Cinética , Lactobacillus rhamnosus/citologia , Soluções
17.
Food Res Int ; 138(Pt B): 109817, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-33288189

RESUMO

The aim of this study was to modulate the physicochemical properties, molecular interactions and microstructure of zein-propylene glycol alginate (PGA) complex nanoparticles for delivery of curcumin with the aid of high pressure microfluidization (HPM) (50-150 MPa) and thermal treatment (45-85 °C). The size of zein-PGA complex nanoparticles was decreased to around 300 nm. It was confirmed that the pressure of 100 MPa and temperature of 75 °C were the optimum parameters to provide a better protection of entrapped curcumin against environmental stresses. The electrostatic interaction, hydrogen bonding and hydrophobic attraction were the dominant driving forces in the formation of the complex nanoparticles. Field emission scanning electron microscopy (FE-SEM) revealed that HPM and thermal treatment facilitated the complex nanoparticles to form a more uniform size and spherical shape. During in vitro gastrointestinal digestion, zein-PGA complex nanoparticles showed excellent gastric stability and sustained-release of curcumin in the small intestine. HPM and thermal treatment showed a synergistic effect on enhancing the bioaccessibility of curcumin entrapped in zein-PGA complex nanoparticles. The findings revealed the influence of HPM and thermal treatment on functional attributes of the complex nanoparticles, which could be utilized to design food grade nanoparticles with desirable stability and digestive properties.


Assuntos
Curcumina , Nanopartículas , Zeína , Alginatos , Digestão , Tamanho da Partícula
18.
Int J Biol Macromol ; 165(Pt B): 2286-2294, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-33096181

RESUMO

The current study developed ethylcellulose (EC) based oleogels with the addition of a surface active ingredient (sorbitan monopalmitate, SP), in order to increase the active loading of curcumin by reducing lipid oxidation, improving curcumin solubility and chemical stability. With the increase in SP content, EC oleogels had more compact gel networks with evenly distributed smaller pores. Rheological analysis revealed that the gels had shear-thinning behavior, and higher concentration of SP contributed to the systems with higher viscosity. The inclusion of SP also worked to reinforce gel strength as determined by frequency sweep, creep recovery and textural analyses. EC oleogels with higher content of SP were capable to hold more liquid oil during centrifugation, and the T2 relaxation time was much lower as determined by NMR. Peroxide value of the oleogels was significantly lower in the systems with SP, and a SP content of 4% or 6% was effective in inhibiting lipid oxidation during storage. When curcumin was incorporated within the gel networks, its effective concentration was more retained with the addition of SP, as no curcumin crystals were detected by DSC during a 9-day storage test, and curcumin had much higher retention when exposed to UV light for 8 h.


Assuntos
Celulose/análogos & derivados , Curcumina/química , Curcumina/farmacologia , Tensoativos/química , Celulose/química , Estabilidade de Medicamentos , Hexoses/química , Óleos/química , Compostos Orgânicos/química , Oxirredução , Peróxidos/química , Reologia , Solubilidade , Raios Ultravioleta
19.
Food Funct ; 11(11): 9973-9983, 2020 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-33118591

RESUMO

The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles were fabricated to deliver resveratrol. TS was firstly introduced to modulate the functional attributes, microstructure, molecular interactions and gastrointestinal digestion of the complex nanoparticles. The size of zein-PGA-TS complex nanoparticles was between 281.9 and 309.7 nm. In the presence of TS, the encapsulation efficiency of resveratrol was significantly elevated from 58.43% to 85.58%. The environmental stability of resveratrol was improved through entrapping into the complex nanoparticles with the rise in TS proportion. Multiple spectroscopic methods revealed that TS altered the micro-environment and secondary structure of the protein. Hydrogen bonds, hydrophobic effects and electrostatic interactions contributed to the formation of complex nanoparticles. Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) patterns showed the amorphous nature of the encapsulated resveratrol. Field emission scanning electron microscopy (FE-SEM) confirmed the globular shape of the nanoparticles and their different aggregation states were dependent on the particle compositions. Moreover, the zein-PGA-TS complex nanoparticles exhibited the best sustained release in the small intestine when the mass ratio of zein to TS was 5 : 1 (23.20% in the stomach and 63.11% in the small intestine). These findings indicated the influence of TS on the properties and applications of the protein-polysaccharide complexes, which provided a new insight into the development of novel food grade nanoparticles with desirable stability and digestion behaviour.


Assuntos
Extratos Vegetais/química , Polissacarídeos/química , Proteínas/química , Resveratrol/química , Saponinas/química , Alginatos/química , Alginatos/metabolismo , Camellia sinensis/química , Digestão , Composição de Medicamentos , Trato Gastrointestinal/química , Trato Gastrointestinal/metabolismo , Humanos , Interações Hidrofóbicas e Hidrofílicas , Modelos Biológicos , Nanopartículas/química , Nanopartículas/metabolismo , Tamanho da Partícula , Extratos Vegetais/metabolismo , Polissacarídeos/metabolismo , Proteínas/metabolismo , Resveratrol/metabolismo , Saponinas/metabolismo , Eletricidade Estática
20.
Int J Biol Macromol ; 164: 2215-2223, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-32755700

RESUMO

The impacts of chitosan with different molecular weight on the formation mechanism, structural characteristics and encapsulating properties of the zein-chitosan nanocomplexes were investigated. The results of dynamic light scattering showed that the mean size of nanocomplexes was increased and became more highly positive charge as the molecular weight of chitosan was increased. Circular dichroism indicated that the secondary structure of zein changed after its combination of chitosan. XRD confirmed that quercetagetin was successfully encapsulated into the nanocomplexes, and FTIR revealed that hydrogen bonding and hydrophobic interaction were the main forces acting among the zein, quercetagetin, and chitosan molecules. The optimized zein-Que-LCH (low molecular weight chitosan) nanocomplexes were relatively small (309 nm), positively charged (+42.5 mV), and had a high entrapment efficiency (94.9%). Moreover, encapsulation of quercetagetin within the nanocomplexes extended its half-life by 3.84- and 2.98- folds during the photo- and thermal-degradation measurements, and effectively modulated its release under simulated gastrointestinal conditions.


Assuntos
Quitosana/química , Flavonas/química , Nanopartículas/química , Zeína/química , Dicroísmo Circular/métodos , Sistemas de Liberação de Medicamentos/métodos , Ligação de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Peso Molecular , Tamanho da Partícula
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