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1.
J Colloid Interface Sci ; 607(Pt 2): 1796-1804, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34600343

RESUMO

Mixed-halide blue perovskites CsPb(Br/Cl)3 are considered promising candidates for developing efficient deep-blue perovskite light-emitting diodes (PeLEDs), but their low photoluminescence quantum yield (PLQY), environmental instability, and poor device performance gravely inhibit their future development. Here, we employ a heteroatomic Cu2+ doping strategy combined with post-treatment Br- anion exchange to prepare high-performance deep-blue perovskites CsPb(Br/Cl)3. The Cu2+ doping strategy significantly decreases the intrinsic chlorine defects, ensuring that the inferior CsPbCl3 quantum dots are transformed into two-dimensional nanosheets with enhanced violet photoluminescence and increased exciton binding energy. Further, with the post-treatment Br- anion exchange, the as-prepared CsPb(Br/Cl)3 nanosheets with more radiation recombination and less ion migration present an enhanced PLQY of 94% and better humidity stability of 30 days. Based on the optimized CsPb(Br/Cl)3, we fabricated deep-blue PeLEDs with luminescence emission at 462 nm, a maximum luminance of 761 cd m-2, and a current density of 205 mA cm-2. This work puts forward a feasible synthesis strategy to prepare efficient and stable mixed-halide blue perovskite CsPb(Br/Cl)3 and related blue PeLEDs, which may promote the further application of mixed-halide perovskites in the blue light range.

2.
Sci Total Environ ; 807(Pt 1): 150487, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34600984

RESUMO

The population of the semi-arid areas of the countries in the East African Rift Valley (EARV) is faced with serious problems associated with the availability and the quality of the drinking water. In these areas, the drinking water supply largely relies on groundwater characterised by elevated fluoride concentration (> 1.5 mg/L), resulting from interactions with the surrounding alkaline volcanic rocks. This geochemical anomaly is often associated with the presence of other naturally occurring potentially toxic elements (PTEs), such as As, Mo, U, V, which are known to cause adverse effects on human health. This study reports on the occurrence of such PTEs in the groundwater on the populated flanks of Mt. Meru, an active volcano situated in the EARV. Our results show that the majority of analysed PTEs (Al, As, Ba, Cd, Cr, Cu, Fe, Mn, Ni, Se, Sr, Pb, and Zn) are within the acceptable limits for drinking purpose in samples collected from wells, springs and tap systems, suggesting that there is no immediate health risk associated with these PTEs. However, some of the samples were found to exceed the WHO tolerance limit for U (> 30 µg/L) and Mo (> 70 µg/L). The sample analysis also revealed that in some of the collected samples, the concentrations of total dissolved solids, Na+ and K+ exceed the permissible limits. The concerning levels of major parameters and PTEs were found to be associated with areas covered with debris avalanche deposits on the northeast flank, and volcanic ash and alluvial deposits on the southwest flanks of the volcano. The study highlights the need to extend the range of elements monitored in the regional groundwater and make a more routine measurement of PTEs to ensure drinking water safety and effective water management measures.

3.
Sci Total Environ ; 807(Pt 1): 150867, 2021 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-34627913

RESUMO

Herein, we attempted to apply an alumina-based bimetallic (Mn-Ce) catalyst as an O3 activator and explored the feasibility of the treatment of hypersaline organic wastewater. Compared with independent O3 (35.00 ± 4.20%), mineralization of ciprofloxacin (CIP) under the Mn-CeOx@γ-Al2O3/O3 (MCAO) process was elevated to 76.04 ± 2.30%. The synergetic corporation among multivalence redox pairs of Mn (III)/Mn (IV), Ce (III)/Ce (IV) promoted the protonation of the surface hydroxyl group (S-OH2+), and subsequently the dominant reactive oxygen species in the MCAO process, OH and O2-, were generated rapidly. However, the mineralization of CIP decreased in MCAO/SO42- system due to the formation of SO4-, which reacted with CIP more slowly (8.4 × 108 M-1 s-1) than OH (4.1 × 109 M-1 s-1). In MCAO/SO42-/Cl- mixture saline conditions, mineralization of CIP was improved at low Cl- concentration (0.5 wt%) due to the generation of Cl, while inhibited with excessive Cl- (≥1.5 wt%) owing to the formation of residual chlorides (Cl2, Cl2- and ClO-). Meanwhile, the MCAO process possessed promising capability to remediate hypersaline wastewater containing dyes, phenol and pesticides, as well as actual salinity-rich wastewater. Based on the above, the present study would provide new insights into hypersaline organic wastewater treatment by the MCAO process.

4.
Theranostics ; 11(19): 9296-9310, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34646371

RESUMO

Mutations in serotonin pathway genes, especially the serotonergic receptor subunit gene HTR3A, are associated with autism. However, the association of HTR3A deficiency with autism and the underlying mechanisms remain unknown. Methods: The Htr3a knockout (KO) mice were generated using transcription activator-like effector nuclease technology. Various behavior tests, including social interaction, social approach task, olfactory habituation/dishabituation, self-grooming, novel object recognition, contextual fear conditioning, elevated plus maze, open field and seizure susceptibility, were performed to assess the phenotypes. Transcriptome sequencing was carried out to search for molecular network and pathways underlying the phenotypes. Electrophysiological recordings, immunoblotting, immunofluorescence staining, immunoprecipitation, and quantitative real-time PCR were performed to verify the potential mechanisms. The N-methyl-D-aspartate receptor (NMDAR) antagonist memantine was used to treat the KO mice for rescuing the phenotypes. Results: The Htr3a KO mouse model showed three phenotypic domains: autistic-like behaviors (including impaired social behavior, cognitive deficits, and increased repetitive self-grooming), impaired memory, and attenuated susceptibility to pentylenetetrazol-induced seizures. We observed enhanced action potential-driven γ-aminobutyric acid-ergic (GABAergic) transmission in pyramidal neurons and decreased excitatory/inhibitory (E/I) ratio using the patch-clamp recording. Transcriptome sequencing on the hippocampus revealed the converged pathways of the dysregulated molecular networks underlying three phenotypic domains with upregulation of NMDAR. We speculated that Htr3a KO promotes an increase in GABA release through NMDAR upregulation. The electrophysiological recordings on hippocampal parvalbumin-positive (PV+) interneuron revealed increased NMDAR current and NMDAR-dependent excitability. The NMDAR antagonist memantine could rescue GABAergic transmission in the hippocampus and ameliorate autistic-like behaviors of the KO mice. Conclusion: Our data indicated that upregulation of the NMDAR in PV+ interneurons may play a critical role in regulating GABAergic input to pyramidal neurons and maybe involve in the pathogenesis of autism associated with HTR3A deficiency. Therefore, we suggest that the NMDAR system could be considered potential therapeutic target for autism.

5.
J Hazard Mater ; 424(Pt A): 127203, 2021 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-34600392

RESUMO

With the deepening of the concept of recycling economy and green chemistry, selective capture of Cu(II) from wastewater by biosorbent and reuse of the spent Cu(II)-loaded adsorbent are of great significance. Herein, we synthesized composite of rice husk (RH) with mesoporous silica MCM-41 (RH@MCM-41) modified by organosilane containing amino and schiff groups as functional monomer and cross-linking agent. The silica modified RH@MCM-41 was employed as supporter to fabricate copper ion-imprinted polymers as absorbents (named as RM-CIIPs) via surface ion imprinting technique. Adsorption isotherms, kinetics, selectivity and mechanism of RM-CIIPs to remove Cu(II) were investigated with respect to different adsorption condition. Furthermore, we explored the catalytic activity of spent Cu(II)-loaded adsorbent in Glaser coupling reaction. Batch adsorption studies revealed that RM-CIIP-3 prepared with functional monomer shows the best adsorption capacity (91.4 mg/g) for Cu(II), and adsorption equilibrium could be reached within 30 min. RM-CIIP-3 exhibited an excellent selectivity for capturing Cu(II) and reusability in six adsorption/desorption cycles. More importantly, the spent Cu(II)-loaded adsorbent could be used as bio-heterogeneous catalyst and afford the desired product (1,4-diphenylbutadiyne) in 99.1% yield. Our research indicates an eco-friendly systematic strategy to utilize the waste material as an adsorbent for removing heavy metals and catalyst for industry.

6.
Front Immunol ; 12: 666594, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34630378

RESUMO

Coxsackievirus B3 (CVB3)-induced viral myocarditis is a common clinical cardiovascular disease without effective available vaccine. In this study, we tried to potentiate the immunoprotection efficacy of our previous CVB3-specific VP1 protein vaccine by introducing a streptococcal protein G-derived, draining lymph nodes (dLNs)-targeting albumin-binding domain (ABD) peptide. We found that compared with the original VP1 vaccine, ABD-fused VP1 (ABD-VP1) vaccine gained the new ability to efficiently bind murine albumin both in vitro and in vivo, possessed a much longer serum half-life in serum and exhibited more abundance in the dLNs after immunization. Accordingly, ABD-VP1 immunization not only significantly facilitated the enrichment and maturation of dendritic cells (DCs), induced higher percentages of IFN-γ+ CD8 + cells in the dLNs, but also robustly promoted VP1-induced T cell proliferation and cytotoxic T lymphocyte (CTL) responses in the spleens. More importantly, ABD-VP1 also elicited higher percentages of protective CD44hi CD62Lhi memory T cells in dLNs and spleens. Consequently, obvious protective effect against viral myocarditis was conferred by ABD-VP1 vaccine compared to the VP1 vaccine, reflected by the less body weight loss, improved cardiac function, alleviated cardiac histomorphological changes and an increased 28-day survival rate. Our results indicated that the ABD might be a promising immune-enhancing regime for vaccine design and development.

7.
Epigenomics ; 13(17): 1347-1358, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34558967

RESUMO

Aim: To determine whether the promoters of long noncoding RNAs (lncRNAs) undergo dynamic changes in DNA methylation during fetal development. Methods: ANOVA and the tissue specificity index were used to identify and validate tissue-specific methylation sites. Age-associated DNA methylation signatures were identified by applying the elastic net method. Results: The lncRNA methylome landscape was characterized in four types of fetal tissue and at three gestational time points, and specific characteristics relative to the tissue of origin and developmental age were identified. Higher levels of lncRNA methylation might be involved in tissue differentiation. LncRNAs harboring age-associated methylation signatures may participate in the fetal developmental process. Conclusion: This study provides novel insights into the role of lncRNA methylomes in fetal tissue specification and development.

8.
Spectrochim Acta A Mol Biomol Spectrosc ; 264: 120327, 2021 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-34474220

RESUMO

In this study, a classification model was established based on near-infrared spectroscopy and random forest method to accurately distinguish three samples of Schisandra chinensis from different habitats. At the same time, the feasibility of fast and effective prediction of polysaccharide contents in Schisandra chinensis by near-infrared spectroscopy combined with chemometrics was evaluated. In this paper, phenol sulfuric acid method was used to determine the content of total polysaccharides in samples, and partial least squares regression algorithm was used to link the spectral information with the reference value. Different spectral pretreatment methods were used to optimize the model to improve its predictability and stability. The results showed that random forest could distinguish these samples accurately, with an accuracy of 97.47%. In the established prediction model, the RMSEC of the optimal model calibration set is 0.0012, and the coefficient of determination R is 0.9976. The RMSEP of prediction set is 0.0024, the coefficient of determination R is 0.9922, and the RPD is 11.36. In general, the method has good stability and applicability, which provides a new analytical method for the identification of Schisandra chinensis origin and quality evaluation.

9.
J Hazard Mater ; 423(Pt B): 127064, 2021 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-34537651

RESUMO

In view of the complexity and diversity of multi-state oils, the development of green and low-cost materials with high selectivity to oils has important ecological significance in the polluted water treatment. Herein, a simple method was proposed to develop large-scale production of superhydrophobic sponges (CPMF200 sponges) for high-efficiency oil/water separation under different complex environments. The as-prepared CPMF200 sponges possessed many superior properties, including high roughness, well-developed porosity, good thermal stability, excellent chemical stability, and superhydrophobic properties (water contact angle is 152°), which is conducive to high oil adsorption capacity (up to 70-179 times of its own weight) and oil-water separation. More importantly, the CPMF400 sponge has an excellent photothermal conversion capability to improve the fluidity of high viscosity oil for oil recovery. Based on a simple synthesis method, it exhibits high-efficiency absorption of multi-state oils and excellent oil-water separation performance and strongly proves their application prospects in treating oily wastewater.

10.
Brief Bioinform ; 2021 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-34581409

RESUMO

Long non-coding RNAs (lncRNAs) that emanate from enhancer regions (defined as enhancer-associated lncRNAs, or elncRNAs) are emerging as critical regulators in disease progression. However, their biological characteristics and clinical relevance have not been fully portrayed. Here, based on the traditional expression quantitative loci (eQTL) and our optimized residual eQTL method, we comprehensively described the genetic effect on elncRNA expression in more than 300 lymphoblastoid cell lines. Meanwhile, a chromatin atlas of elncRNAs relative to the genetic regulation state was depicted. By applying the maximum likelihood estimate method, we successfully identified causal elncRNAs for protein-coding gene expression reprogramming and showed their associated single nucleotide polymorphisms (SNPs) favor binding of transcription factors. Further epigenome analysis revealed two immune-associated elncRNAs AL662844.4 and LINC01215 possess high levels of H3K27ac and H3K4me1 in human cancer. Besides, pan-cancer analysis of 3D genome, transcriptome, and regulatome data showed they potentially regulate tumor-immune cell interaction through affecting MHC class I genes and CD47, respectively. Moreover, our study showed there exist associations between elncRNA and patient survival. Finally, we made a user-friendly web interface available for exploring the regulatory relationship of SNP-elncRNA-protein-coding gene triplets (http://bio-bigdata.hrbmu.edu.cn/elncVarReg). Our study provides critical mechanistic insights for elncRNA function and illustrates their implications in human cancer.

11.
Nanoscale ; 13(37): 15647-15658, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34532728

RESUMO

Nanocapsules are a promising controlled release formulation for foliar pest control. However, the complicated process and high cost limit widespread use in agriculture, so a simpler and more convenient preparation system is urgently needed. Meanwhile, under complex field conditions, the advantageous mechanism of the nanosize effect and sustained release have no quantitative and detailed study. In this study, a reactive emulsifier (OP-10) is used to participate in the interfacial polymerization of the nanoemulsion, and polymer nanocapsules loaded with lambda-cyhalothrin (NCS@LC) are quickly and easily prepared to study the efficacy and synergistic mechanism of foliar pest control. As a result, the nanocapsule is about 150 nm with a stable core-shell structure. The nanoscale state increases the distribution and adhesion of the particles on the leaf surface, which increases the contact efficiency of pesticides under the different physiological stages and behavioral activities of the target organism. The shell structure provides sustained release characteristics and increases the UV resistance by about 2.5 times for pesticides. Compared with microcapsules loaded with lambda-cyhalothrin (CS@LC), NCS@LC not only shows rapid and synergistic insecticidal efficacy but also provides sustained insecticidal efficacy. The mortality of NCS is 3.4 times that of the nanosized emulsion in water (NEW) at the lowest concentration (0.5 mg L-1), and the control efficacy remained 77.3% after 7 days. Compared with NEW, NCS@LC provides excellent field efficacy, while LC50 for zebrafish is only 0.68 times without increasing the aquatic toxicity risk.


Assuntos
Inseticidas , Nanocápsulas , Piretrinas , Animais , Nanocápsulas/toxicidade , Nitrilas , Peixe-Zebra
12.
ACS Appl Mater Interfaces ; 13(38): 45119-45129, 2021 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-34530608

RESUMO

Rapid advances in nanotechnology have opened up innovative trails to break through the current limitation in clinical treatments of cancer and other critical diseases that plague human beings. Ultrasmall iron oxide nanoparticles (USIO NPs) with sizes smaller than 5 nm have been emerging as a novel category of nanomaterials with increasing interest in the biomedical domains. To overcome their intrinsic shortcomings, naked USIO NPs can be functionalized, clustered, assembled, or incorporated with other nanomaterials to generate various kinds of intelligent nanoplatforms for single-mode or dynamic magnetic resonance (MR) imaging, multimode imaging, as well as imaging-guided precision therapy. In this spotlight on applications, first, we propose the principal aspects in the design and application of USIO NPs for biomedical uses. Second, we cover the recent design strategies of USIO NP-based nanoplatforms mainly developed by our group, describe the rationale on the combination of other functional materials with USIO NPs, and review their resultant applications in theranostics. In addition, we provide herein a perspective on the possible future directions toward USIO NP-based nanoplatforms as smart nanomedicines.

13.
Phytomedicine ; : 153727, 2021 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-34535372

RESUMO

BACKGROUND: Globally, lung cancer is the leading cause of cancer associated mortalities. The current conventional chemotherapy remains the preferred treatment option for lung cancer, as surgical resection plays little role in the treatment of over 75% of lung cancer patients. Therefore, there is a need to develop novel potential therapeutic drugs or adjuvants with a high efficiency and safety against lung cancer. Scutellaria baicalensis Georgi, a common Chinese medicinal herb that has been in use for more than 2000 years, has recently been shown to possess significant activities against lung cancer. However, current research progress on pharmacological effects and relevant molecular mechanisms of S. baicalensis in lung cancer therapy have not been systematically summarized. PURPOSE: This review aimed at elucidating on the anti-lung cancer mechanisms and antitumor efficacies of S. baicalensis as well as its active ingredients, and providing a valuable reference for further investigation in this field. METHODS: We used "Scutellaria baicalensis" or the name of the compound in S. baicalensis, in combination with "lung cancer" as key words to systematically search for relevant literature from the Web of Science and PubMed databases. Publications that investigated molecular mechanisms were the only ones selected for analysis. The PRISMA guidelines were followed. RESULTS: Fifty-four publications met the inclusion criteria for this study. Five anti-lung cancer mechanisms of S. baicalensis and its constituent components are discussed. These mechanisms include apoptosis induction, cell-cycle arrest, suppression of proliferation, blockade of invasion and metastasis, and overcoming drug-resistance. These compounds exhibited high antitumor efficacies and safety against lung cancer xenografts. CONCLUSION: Studies should aim at elucidating on the anti-cancer mechanisms of S. baicalensis to achieve the ultimate goal of lung cancer therapy.

14.
J Magn Reson Imaging ; 2021 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-34549860

RESUMO

BACKGROUND: Type 2 diabetes mellitus (T2DM) and obesity often coexist and together contribute to left atrial (LA) functional abnormalities. However, little is known about the impact of body mass index (BMI) on LA strains measured using cardiac magnetic resonance feature tracking (MR-FT). PURPOSE: To investigate the additive effect of BMI on LA functional remodeling using MR-FT as well as to explore abnormal atrioventricular interaction in T2DM patients. STUDY TYPE: Retrospective. POPULATION: One hundred and fifty-nine T2DM patients (53, 34, and 72 normal-weight, overweight, and obese individuals) and 105 non-diabetic controls (46, 32, and 27 normal-weight, overweight, and obese individuals). FIELD STRENGTH/SEQUENCE: 3.0 T/balanced steady-state free precession. ASSESSMENT: LA reservoir strain (εs ), conduit strain (εe ), and active strain (εa ) and their corresponding strain rates (SRs, SRe, SRa) were measured together with left ventricular (LV) radial, circumferential, and longitudinal peak strain, peak systolic strain rate, and peak diastolic strain rate. STATISTICAL TESTS: Student's t-test or Mann-Whitney U test, one-way ANOVA, univariate and multivariate linear regression. A P value <0.05 was considered statistically significant. RESULTS: Compared to controls in the same BMI category, T2DM patients had significantly decreased reservoir and conduit function, while pump function was not significantly different (εa and SRa: P = 0.757 and 0.583 for normal-weight, P = 0.171 and 0.426 for overweight, P = 0.067 and 0.330 for obese). LA strains were significantly correlated with BMI (r = -0.346 for εs , -0.345 for εe ) in T2DM patients. Multivariable linear regression analysis indicated that BMI was independently associated with LA εs and εe , LV global longitudinal strain was independently associated with LA εs and εa , and LV circumferential peak diastolic strain rate was independently associated with LA εe . DATA CONCLUSION: These findings suggest that the coexistence of increased body weight and T2DM patients can exacerbate the impairment of LA strains and indicate abnormal atrioventricular interactions. LEVEL OF EVIDENCE: 3 TECHNICAL EFFICACY: Stage 3.

15.
J Appl Toxicol ; 2021 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-34550611

RESUMO

The effects of low-dose radiation (LDR, ≤0.1 Gy) on living organisms have been the hot areas of radiation biology but do not reach a definitive conclusion yet. So far, few studies have adequately accounted for the male reproductive system responses to LDR, particularly the regulation of testosterone content. Hence, this study was designed to evaluate the effects of LDR on Leydig cells and testicular tissue, especially the ability to synthesize testosterone. We found that less than 0.2-Gy 60 Co gamma rays did not cause significant changes in the hemogram index and the body weight; also, pathological examination did not find obvious structural alterations in testis, epididymis, and other radiation-sensitive organs. Consistently, the results from in vitro showed that only more than 0.5-Gy gamma rays could induce remarkable DNA damage, cycle arrest, and apoptosis. Notably, LDR disturbed the contents of testosterone in mice serums and culture supernatants of TM3 cells and dose dependently increased the expression of 3ß-HSD. After cotreatment with trilostane (Tril), the inhibitor of 3ß-HSD, increased testosterone could be partially reversed. Besides, DNA damage repair-related enzymes, including DNMT1, DNMT3B, and Sirt1, were increased in irradiated TM3 cells, accompanying by evident demethylation in the gene body of 3ß-HSD. In conclusion, our results strongly suggest that LDR could induce obvious perturbation in the synthesis of testosterone without causing organic damage, during which DNA demethylation modification of 3ß-HSD might play a crucial role and would be a potential target to prevent LDR-induced male reproductive damage.

16.
Hum Brain Mapp ; 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34469016

RESUMO

Current theories of bilingualism disagree on the extent to which separate brain regions are used to maintain or process one's first and second language. The present study took a novel multivariate approach to address this question. We examined whether bilinguals maintain distinct neural representations of two languages; specifically, we tested whether brain areas that are involved in processing word meaning in either language are reliably representing each language differently, and whether language representation is influenced by individual differences in proficiency level and age of acquisition (AoA) of L2. Thirty-one English-Mandarin bilingual adults performed a picture-word matching task in both languages. We then used representational similarity analysis to examine which brain regions reliably showed different patterns of activity for each language. We found that both proficiency and AoA predicted dissimilarity between language representations in several brain areas within the language network as well as several regions of the ventral visual pathway, demonstrating that top-down language knowledge and individual language experience shapes concept representation in this processing stream. The results support the model of an integrated language system in bilinguals, along with a novel description of how representations for each language change with proficiency level and L2 AoA.

17.
Int J Radiat Biol ; : 1-9, 2021 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-34402389

RESUMO

PURPOSE: Ovarian cancer is one of the most common gynecological cancers in women with a low 5-year survival rate. Evaluation of hyaluronic acid-binding protein 1 (HABP1) level can provide important information for the diagnosis and treatment of ovarian cancer. In this study, we designed a novel HABP1 probe based on 99mTc-radiolabeled small-interference RNA (siRNA) for detecting HABP1 expression noninvasively in vivo, thereby providing a new method for its diagnosis and treatment. METHODS: A specific siHABP1 was selected because of its targetability and silencing effect. A negative control siRNA (NCsiRNA) with no homology with the human genome was used. SiHABP1 and NCsiRNA were radiolabeled with 99mTc using the bifunctional chelating agent hydrazinonicotinamide (HYNIC). The radiochemical purity and in vitro stability of the probe were determined by HPLC. The binding activity was measured by western blotting (WB) and RT-PCR. The HABP1-overexpressing human ovarian cancer cell line HO-8910 was used for cell uptake experiments, which were performed with or without transfection and measured with a gamma counter. HO8910-bearing mice were imaged at 1, 4, and 10 h, and biodistribution analysis was performed at 1, 4, 6, and 10 h after injection of 99mTc-HYNIC-siRNA. RESULTS: 99mTc-HYNIC-siHABP1 had high radiochemical purity and good in vitro stability, and showed the same binding capacity and silencing effect as siHABP1. SPECT imaging showed that tumors were clearly visualized at 10 h after injection of 99mTc-HYNIC-siHABP1 but not after 99mTc-HYNIC-NCsiRNA, implying specific binding. The biodistribution results were consistent with those of SPECT imaging. CONCLUSIONS: We showed that 99mTc-HYNIC-siHABP1 is a feasible probe for the noninvasive visualization of HABP1 expression in ovarian cancer.

18.
J Healthc Eng ; 2021: 4845792, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34422243

RESUMO

Objective: The role of deep learning-based echocardiography in the diagnosis and evaluation of the effects of routine anti-heart-failure Western medicines was investigated in elderly patients with acute left heart failure (ALHF). Methods: A total of 80 elderly patients with ALHF admitted to Affiliated Hangzhou First People's Hospital from August 2017 to February 2019 were selected as the research objects, and they were divided randomly into a control group and an observation group, with 40 cases in each group. Then, a deep convolutional neural network (DCNN) algorithm model was established, and image preprocessing was carried out. The binarized threshold segmentation was used for denoising, and the image was for illumination processing to balance the overall brightness of the image and increase the usable data of the model, so as to reduce the interference of subsequent feature extraction. Finally, the detailed module of deep convolutional layer network algorithm was realized. Besides, the patients from the control group were given routine echocardiography, and the observation group underwent echocardiography based on deep learning algorithm. Moreover, the hospitalization status of patients from the two groups was observed and recorded, including mortality rate, rehospitalization rate, average length of hospitalization, and hospitalization expenses. The diagnostic accuracy of the two examination methods was compared, and the electrocardiogram (ECG) and echocardiographic parameters as well as patients' quality of life were recorded in both groups at the basic state and 5 months after drug treatment. Results: After comparison, the rehospitalization rate and mortality rate of the observation group were lower than the rates of the control group, but the diagnostic accuracy was higher than that of the control group. However, the difference between the two groups of patients was not statistically marked (P > 0.05). The length and expenses of hospitalization of the observation group were both less than those of the control group. The specificity, sensitivity, and accuracy of the examination methods in the observation group were higher than those of the control group, and the differences were statistically marked (P < 0.05). There was a statistically great difference between the interventricular delay (IVD) of the echocardiographic parameters of patients from the two groups at the basic state and the left ventricular electromechanical delay (LVEMD) parameter values after 5 months of treatment (P < 0.05), but there was no significant difference in the other parameters. After treatment, the quality of life of patients from the two groups was improved, while the observation group was more marked than the control group (P < 0.05). Conclusion: Echocardiography based on deep learning algorithm had high diagnostic accuracy and could reduce the possibility of cardiovascular events in patients with heart failure, so as to decrease the mortality rate and diagnosis and treatment costs. Moreover, it had an obvious diagnostic effect, which was conducive to the timely detection and treatment of clinical diseases.

19.
Macromol Biosci ; : e2100103, 2021 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-34405955

RESUMO

Chronic wound infections have caused an increasing number of deaths and economic burden, which necessitates wound treatment options. Hitherto, the development of functional wound dressings has achieved reasonable progress. Antibacterial agents, growth factors, and miRNAs are incorporated in different wound dressings to treat various types of wounds. As an effective antimicrobial agent and emerging wound healing therapeutic, antimicrobial peptides (AMPs) have attracted significant attention. The present study focuses on the application of AMPs in wound healing and discusses the types, properties and formulation strategies of AMPs used for wound healing. In addition, the clinical trial and the current status of studies on "antimicrobial peptides and wound healing" are elaborated through bibliometrics. Also, the challenges and opportunities for further development and utilization of AMP formulations in wound healing are discussed.

20.
Hepatology ; 2021 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-34387904

RESUMO

BACKGROUND & AIMS: Peroxisome proliferator-activated receptor α (PPARα, NR1C1) is a ligand-activated nuclear receptor involved in the regulation of lipid catabolism and energy homeostasis. PPARα activation induces hepatomegaly and plays an important role in liver regeneration, but the underlying mechanisms remain unclear. APPROACH & RESULTS: In this study, the effect of PPARα activation on liver enlargement and regeneration was investigated in several strains of genetically-modified mice. PPARα activation by the specific agonist WY-14643 significantly induced hepatomegaly and accelerated liver regeneration after 70% partial hepatectomy (PHx) in wild-type mice and Pparafl/fl mice, while these effects were abolished in hepatocyte-specific Pparα-deficient (PparaΔHep ) mice. Moreover, PPARα activation promoted hepatocyte hypertrophy around the central vein area and hepatocyte proliferation around the portal vein area. Mechanistically, PPARα activation regulated expression of yes-associated protein (YAP) and its downstream targets (CTGF, CYR61 and ANKRD1) as well as proliferation-related proteins (CCNA1, CCND1 and CCNE1). Binding of YAP with the PPARα E domain was critical for the interaction between YAP and PPARα. PPARα activation further induced nuclear translocation of YAP. Disruption of the YAP-transcriptional enhancer factor domain family member (TEAD) association significantly suppressed PPARα-induced hepatomegaly, and hepatocyte enlargement and proliferation. In addition, PPARα failed to induce hepatomegaly in AAV-Yap shRNA-treated mice and liver-specific Yap-deficient (YapΔHep ) mice. Blockade of YAP signaling abolished PPARα-induced hepatocyte hypertrophy around the central vein area and hepatocyte proliferation around the portal vein area. CONCLUSIONS: This study revealed a novel function of PPARα in regulating liver size and liver regeneration via activation of the YAP-TEAD signaling pathway. These findings have implications for understanding the physiological functions of PPARα and suggest its potential for manipulation of liver size and liver regeneration.

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