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1.
Water Res ; 208: 117870, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34823084

RESUMO

Household, or point-of-use (POU), water treatments are effective alternatives to provide safe drinking water in locations isolated from a water treatment and distribution network. The household slow sand filter (HSSF) is amongst the most effective and promising POU alternatives available today. Since the development of the patented biosand filter in the early 1990s, the HSSF has undergone a number of modifications and adaptations to improve its performance, making it easier to operate and increase users' acceptability. Consequently, several HSSF models are currently available, including those with alternative designs and constant operation, in addition to the patented ones. In this scenario, the present paper aims to provide a comprehensive overview from the earliest to the most recent publications on the HSSF design, operational parameters, removal mechanisms, efficiency, and field experiences. Based on a critical discussion, this paper will contribute to expanding the knowledge of HSSF in the peer-reviewed literature.


Assuntos
Filtração , Purificação da Água , Características da Família , Dióxido de Silício
2.
Eur J Paediatr Dent ; 21(3): 187-191, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32893649

RESUMO

AIM: Low attendance and abandonment of dental treatment caused by dental fear varies from 6% to 20%. Various specific scales have been used to evaluate and measure dental fear, like Children´s Fear Survey Schedule - Dental Subscale, which is considered the "gold standard", due to its efficacy and validity. However, not all the studies that have been conducted have performed the Exploratory Factorial and Confirmation Analysis to infer the validity of the scale's content, which is why this study has as its main objective to analyse the confidentiality and validation of this scale. MATERIALS AND METHODS: In a sample of 163 school age children, from 6 to 12 years old, of both genders, from a public school, a Children's Fear Survey Schedule - Dental Subscale was applied to measure dental fear. The internal consistency of the scale was estimated with the Cronbach's alpha, we also performed the Exploratory Factorial Analysis with the method of the main component with Varimax rotation and the Confirmatory Factorial Analysis to confirm the structure of the factors. RESULTS: The Cronbach's alpha was obtained with a .837 (p?.05). The adequate coefficients were determined with the Kaiser-Meyer-Olkin (KMO = .843) and the Bartlett's test of specificity (?2 = 565,325, p= .000), in which there was a good adequate data, expressing an appropriate and intercorrelation between items or strongly related. The factorial structure showed four factors extracted, 4 factors explain the 53.57% of the accumulated variation; 14 items were above the .40. The statistics of the second and third model obtained adequate values in the statistics of goodness of fit index. CONCLUSION: The Mexican version of the CFSS-DS in the present study gives us data about the adaptation and validation of the dental fear scale of CFSSS-DS by the Exploratory Factorial Analysis through a model of structural equations. This means that this instrument is a trusting and valid tool to measure dental fear in the children's population of Mexico.


Assuntos
Ansiedade ao Tratamento Odontológico , Medo , Criança , Feminino , Humanos , Masculino , México , Reprodutibilidade dos Testes , Inquéritos e Questionários
3.
Hum Exp Toxicol ; 38(8): 951-961, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31018701

RESUMO

The transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2) is a master regulator of a battery of antioxidant and detoxificant genes with cytoprotective function. Since Nrf2 inactivation is necessary for the complete execution of apoptosis in the presence of extensive cellular damage caused by oxidative stress, constant activation of Nrf2 may protect tumoral cells from apoptosis. The tumor suppressor gene p53 has been suggested to participate in apoptosis-related repression of Nrf2. Thus, we studied the inactivation of Nrf2 during oxidant-induced apoptosis in a p53 dysfunctional cellular model. Using curcumin dose-response assay and time-response assay in an immortalized lymphoblastoid cell line (control line 45), we observed a time-dependent increase in apoptotic markers such as deoxyribonucleic acid (DNA) fragmentation, phosphatidylserine exposure, and caspase-3, caspase-9 and poly (ADP-ribose) polymerases (PARP) cleavage. Interestingly, at early times of exposure to a proapoptotic dose of curcumin (15 µM), we observed nuclear accumulation of Nrf2 and the expression of Nrf2 target genes, whereas at late exposure times we found a reduction of total and nuclear protein levels of Nrf2 as well as downregulation of Nrf2 target genes in the absence of p53 activation. These data suggest that apoptosis-related inactivation of Nrf2 could occur in a p53 dysfunctional background, opening the possible occurrence of p53-independent mechanism to explain Nrf2 inactivation during apoptosis.


Assuntos
Apoptose/efeitos dos fármacos , Curcumina/farmacologia , Fator 2 Relacionado a NF-E2/antagonistas & inibidores , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Humanos , Fator 2 Relacionado a NF-E2/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Proteína Supressora de Tumor p53/metabolismo
4.
Behav Brain Res ; 357-358: 98-103, 2019 01 14.
Artigo em Inglês | MEDLINE | ID: mdl-29330003

RESUMO

Previously the effects (0.01-3.0 mg/kg) of post-training SB-699551 (a 5-HT5A receptor antagonist) were reported in the associative learning task of autoshaping, showing that SB-699551 (0.1 mg/kg) decreased lever-press conditioned responses (CR) during short-term (STM; 1.5-h) or (3.0 mg/kg) long-term memory (LTM; 24-h); relative to the vehicle animals. Moreover, as pro-cognitive efficacy of SB-699551 was reported in the ketamine-model of schizophrenia. Hence, firstly aiming improving performance (conditioned response, CR), in this work autoshaping lever-press vs. nose-poke response was compared; secondly, new set of animals were randomly assigned to SB-699551 plus forgetting or amnesia protocols. Results show that the nose-poke operandum reduced inter-individual variance, increased CR and produced a progressive CR until 48-h. After one week of no training/testing sessions (i.e., interruption of 216 h), the forgetting was observed; i.e., the CR% of control-saline group significantly decreased. In contrast, SB-699551 at 0.3 and 3.0 mg/kg prevents forgetting. Additionally, as previously reported the non-competitive NMDA receptor antagonist dizocilpine (0.2 mg/kg) or the non-selective cholinergic antagonist scopolamine (0.3 mg/kg) decreased CR in STM. SB-699551 (0.3 mg/kg) alone also produced amnesia-like effect. Co-administration of SB-699551-dizocilpine or SB-699551-scopolamine reversed the SB-699551 induced-amnesic effects in LTM (24-h). Nose-poke seems to be a reliable operandum. The anti-amnesic and anti-forgetting mechanisms of amnesic SB-699551-dose remain unclear. The present findings are consistent with the notion that low doses of 5-HT5A receptor antagonists might be useful for reversing memory deficits associated to forgetting and amnesia. Of course, further experiments are necessary.


Assuntos
Amnésia/metabolismo , Memória/fisiologia , Receptores de Serotonina/metabolismo , Antagonistas da Serotonina/uso terapêutico , Amnésia/induzido quimicamente , Amnésia/tratamento farmacológico , Análise de Variância , Animais , Compostos de Bifenilo/uso terapêutico , Antagonistas Colinérgicos/toxicidade , Condicionamento Clássico/efeitos dos fármacos , Condicionamento Operante/efeitos dos fármacos , Modelos Animais de Doenças , Maleato de Dizocilpina/farmacologia , Antagonistas de Aminoácidos Excitatórios/farmacologia , Comportamento Exploratório/efeitos dos fármacos , Extinção Psicológica/efeitos dos fármacos , Masculino , Memória/efeitos dos fármacos , Ratos , Ratos Wistar , Escopolamina/toxicidade
5.
Int J Hyg Environ Health ; 221(4): 734-741, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29706434

RESUMO

Microcosms are useful tools for understanding the survival and fate of enteric viruses in aquatic environments. This study set out to determine the stability of infectious enteric viruses in an aquatic environment using a laboratory scale microcosm. Sediment and overlaying water were collected from a lagoon and inoculated with known concentrations of recombinant adenovirus (AdV-GFP) and murine norovirus (MNV-1). Infectious particles of these viruses were measured using fluorescence microscopy (AdV-GFP) or the plaque assay method (MNV-1), over 85 days in two different conditions: under natural sunlight and in fully darkened environments. The time required to reach one log reduction in viral titres (T90) of viable viruses in a natural condition microcosm for AdV-GFP and MNV-1 was shorter than in a dark condition microcosm. There was also a negative correlation between the temperature and infectivity of these viruses in both water and sediment samples. Considering that microcosms aim to mimic natural environment conditions and that AdV-GFP and MNV-1 are excellent surrogates for measuring the infectivity of the respective viruses strains, the results presented here have the potential to be applied in future health hazard studies and also would be useful for future climate scenarios.


Assuntos
Adenoviridae , Norovirus , Poluentes da Água , Adenoviridae/fisiologia , Animais , Água Doce/virologia , Sedimentos Geológicos/virologia , Células HEK293 , Humanos , Camundongos , Microscopia de Fluorescência , Norovirus/fisiologia , Células RAW 264.7 , Luz Solar , Temperatura , Cultura de Vírus , Microbiologia da Água
6.
Meat Sci ; 123: 45-49, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27614179

RESUMO

To evaluate the effect of immunocastration on carcass and meat characteristics, Holstein bulls aged between 7 and 8months with a live weight of 232±1.19kg were given two separate treatments, placebo (intact bulls) versus Bopriva, and then slaughtered after approximately 239days of fattening. While the testosterone levels in intact bulls remained at 0.42ng/ml throughout the study, by day 181, differences (P<0.05) were observed in immunized bulls, with values of 0.21ng/ml. The carcasses of animals treated with Bopriva recorded both a higher hot carcass weight (HCW) and a cold carcass weight (CCW), as well as higher dorsal fat density, marbling and KPH (P<0.05); however, no differences (P>0.05) were observed in the Longissimus lumborum area. No significant differences (P>0.05) were recorded between the treatments for pH, L*, a*, b* C* and H*. The carcasses of the animals treated with Bopriva were heavier, with higher dorsal fat density and marbling score.


Assuntos
Castração/métodos , Qualidade dos Alimentos , Imunização/veterinária , Carne Vermelha/análise , Vacinas Anticoncepcionais/administração & dosagem , Animais , Animais Recém-Nascidos , Bovinos/imunologia , Cor , Concentração de Íons de Hidrogênio , Masculino , Músculo Esquelético/química , Testosterona/sangue
7.
J Cardiovasc Surg (Torino) ; 56(5): 775-85, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25868972

RESUMO

Critical limb ischemia (CLI) encompasses the most extreme end of the peripheral artery disease (PAD) spectrum leading to significant morbidity and mortality. CLI is defined as greater than 2 weeks of extremity rest pain, ulcers or extremity gangrene, secondary to objectively proven peripheral artery disease. Corresponding to Fontaine Stages III/IV and Rutherford category IV through VI, CLI is a complex disease comprising of both macrovascular and microvascular systems with inconsistent historical data on optimal treatment. CLI is distinct from intermittent claudication with different goals of treatment, however in both groups risk factor modification is of the utmost importance involving tobacco cessation, and treatment of underlying conditions like diabetes mellitus, hyperlipidemia and hypertension. In CLI, medical therapy involves wound care and also consists of antiplatelet therapy, anti-inflammatory therapy including statin use or ACE inhibitors. Surgical therapies include distal bypass surgery, thromboendartectomy and amputation. Endovascular techniques include percutaneous transluminal angioplasty, bare metal stents, atherectomy, drug-coated balloon and drug-eluting stents. CLI is considered the end-stage of PAD, requiring a thoughtful and multidisciplinary approach, risk-benefit analysis and treatment of comorbid conditions. Conservative and surgical treatments, along with endovascular techniques, have allowed excellent opportunities for treating complicated patients for wound healing and limb salvage.


Assuntos
Fármacos Cardiovasculares/uso terapêutico , Procedimentos Endovasculares , Isquemia/terapia , Doença Arterial Periférica/terapia , Comportamento de Redução do Risco , Procedimentos Cirúrgicos Vasculares , Amputação , Fármacos Cardiovasculares/efeitos adversos , Estado Terminal , Procedimentos Endovasculares/efeitos adversos , Humanos , Isquemia/diagnóstico , Isquemia/epidemiologia , Salvamento de Membro , Doença Arterial Periférica/diagnóstico , Doença Arterial Periférica/epidemiologia , Fatores de Risco , Resultado do Tratamento , Procedimentos Cirúrgicos Vasculares/efeitos adversos , Cicatrização
8.
Genet Mol Res ; 13(1): 2268-77, 2014 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-24737475

RESUMO

Although polycrosses have been used to test the potential of cross-combination of a large number of sugarcane parents, the male parent of the half-sib progenies produced is unknown. The present study aimed to integrate the molecular marker technology to the sugarcane polycross approach by the application of microsatellite markers to identify the male parent of 41 elite clones derived from polycross families. Ten microsatellite [single sequence repeats (SSRs)] primer pairs were used to identify the most likely male parent considering markers present in the selected clone but absent in the female parent. The number of alleles generated by the 10 microsatellite primer pairs ranged from 102 (cross-pollination lantern 4) to 120 (cross-pollination lantern 2) with an average of 113.25 alleles per SSR. The average genetic similarity among the involved parents in the polycrosses was 45.9%. The results of the analysis of the SSR markers absent in the female parent and present only in the selected clone as well as the genetic similarity values allowed the identification of the most likely male parent in 73% of the total clones evaluated and also to detect probable contaminations. The obtained results highlight the importance of using molecular marker technology in the identification and confirmation of the male parent of high-performance clones derived from polycrosses in the sugarcane breeding programs.


Assuntos
Cruzamentos Genéticos , Repetições de Microssatélites , Saccharum/genética , Alelos , Análise por Conglomerados , Impressões Digitais de DNA , Polinização , Polimorfismo Genético
9.
J Appl Microbiol ; 113(6): 1554-63, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22967243

RESUMO

AIMS: To evaluate the stability in seawater of human adenovirus (HAdV2), murine norovirus (MNV-1) and hepatitis A virus (HAV) in a shellfish depuration system with and without ultraviolet (UV) treatment. METHODS AND RESULTS: Seawater was seeded with viruses and disinfected using a 36 W lamp. Samples were collected at 24, 48, 72, 96 and 120 h; viruses were concentrated and the viral decay was evaluated using molecular and cell culture methods. Based on the molecular results, at 120 h of disinfection, there was a reduction of more than 3 log(10) for HAdV2 and HAV; MNV-1, a 4.5 log(10) reduction was observed at 72 h. Infectious MNV-1 was not detected after 72 h of treatment; while HAdV2 remained infectious. Seawater not treated demonstrated a progressive viral reduction for the three viruses tested. CONCLUSIONS: The UV reduced the number of viral particles, and the results indicate there is natural and gradual decrease of viral load and viability in seawater. SIGNIFICANCE AND IMPACT OF THE STUDY: UV irradiation is the method of choice for shellfish depuration in many countries; this work showed useful information about the viral stability in seawater and application of UV to water disinfection to be used in shellfish depuration tanks.


Assuntos
Adenovírus Humanos/efeitos da radiação , Desinfecção/métodos , Vírus da Hepatite A/efeitos da radiação , Norovirus/efeitos da radiação , Água do Mar/virologia , Raios Ultravioleta , Animais , Aquicultura/métodos , Linhagem Celular Tumoral , DNA Viral/isolamento & purificação , Humanos , Moluscos , RNA Viral/isolamento & purificação , Carga Viral , Ensaio de Placa Viral , Inativação de Vírus
10.
J Water Health ; 10(3): 445-52, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22960488

RESUMO

Animal and human wastewater can potentially contaminate water sources and the treatment of drinking water may not effectively remove all contaminants, especially viruses. The purpose of the present study was to evaluate the viral contamination of water used for human and animal consumption in the city of Concórdia, located in southern Brazil. Porcine circovirus type 2 (PCV2), porcine adenovirus (PAdV), human adenovirus (HAdV) and human norovirus (NoV) were searched for using quantitative polymerase chain reaction (qPCR). HAdV-positive samples were tested for viral infectivity by plaque assay. The qPCR results showed that PAdV, PCV2 and HAdV genetic material were present in all sampling sites. NoV was absent in all samples. The presence of genetic material from PAdV and PCV2 was detected in 30% and 45% of the 36 analyzed samples, respectively, with an average of 10(2) gc mL(-1) for PAdV and 10(4) gc mL(-1) for PCV2. HAdV was present in 100% of the samples, with an average of 10(4) gc mL(-1). However, in plaque assay, only 36% of the samples were positive. As viable particles of HAdV were found in drinking water, these results confirm that swine manure and human sewage impact surface water and groundwater, endangering water quality and indicating a potential risk to public health.


Assuntos
Adenoviridae/isolamento & purificação , Circovirus/isolamento & purificação , Norovirus/isolamento & purificação , Doenças dos Suínos/virologia , Microbiologia da Água , Adenoviridae/classificação , Animais , Brasil , Água Potável , Humanos , Norovirus/classificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Suínos , Doenças dos Suínos/epidemiologia , Abastecimento de Água
11.
Res Vet Sci ; 93(3): 1520-4, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22405906

RESUMO

Samples were collected at the effluent of two swine manure treatment systems and were analyzed by qPCR to determine the presence and amounts of porcine circovirus (PCV2) genetic material. ST cells were inoculated with the positive samples to evaluate virus viability and for viral genotyping. Twenty-five water samples were collected monthly from treated effluent (March 2009 to December 2010). The PCV2 genome was identified by qPCR in 60% of the samples, and all of the positive samples were able to infect ST cells in vitro. Positive samples were genotyped and 60% of them were positive for both PCV2a and PCV2b, 20% were positive for genotype 2a, and 20% were positive for genotype 2b. Our results suggest that these viruses were able to resist the regular wastewater treatment, and this finding demonstrates the necessity of adding a virus inactivation step to the treatment system to guarantee the safety of water reuse.


Assuntos
Circovirus/fisiologia , Fezes/virologia , Cultura de Vírus/métodos , Animais , Linhagem Celular , Circovirus/genética , Genoma Viral , Genótipo , Masculino , Reação em Cadeia da Polimerase/métodos , Reação em Cadeia da Polimerase/veterinária , Testículo/citologia , Fatores de Tempo , Eliminação de Resíduos Líquidos
12.
Res Vet Sci ; 93(1): 538-43, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21872287

RESUMO

Samples collected from two swine manure treatment systems including: swine manure treatment system and demonstrative unit (SMTS and DU), were analyzed by qPCR to quantify the amount of porcine adenovirus (PAdV) and porcine circovirus (PCV2) present. Positive samples were tested for virus integrity using DNase assay. Fifty-six water samples were collected monthly from March 2009 to May 2010. PAdV genome was found 66% of the samples in the SMTS and in 78% of the samples in the DU system. PCV2 was detected in 96% of samples collected from the SMTS system and in 86% of samples from DU. DNase assay revealed that there were undamaged virus particles of both PAdV and PCV2 in all sampling sites in the SMTS. However, undamaged particles of both viruses were detected in samples from the DU system in the affluent and middle sites, though undamaged PCV2 was absent in the effluent samples.


Assuntos
Infecções por Adenoviridae/veterinária , Adenovirus Suínos , Infecções por Circoviridae/veterinária , Circovirus , Esterco/virologia , Doenças dos Suínos/virologia , Microbiologia da Água , Infecções por Adenoviridae/virologia , Adenovirus Suínos/genética , Animais , Infecções por Circoviridae/virologia , Circovirus/genética , Desoxirribonucleases/metabolismo , Genoma Viral/genética , Reação em Cadeia da Polimerase/veterinária , Eliminação de Resíduos , Estações do Ano , Suínos/virologia
13.
Mar Pollut Bull ; 64(1): 40-48, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22104718

RESUMO

The aim of this study was to assess the impact of sewage discharge on coastal waters by evaluating the influence of physicochemical parameters on the presence of enteric microorganisms in seawater samples collected from 11 beaches in Florianopolis, Santa Catarina, Brazil, over a one-year period (August 2009 to July 2010). Samples were assessed for the presence of human adenoviruses (HAdV), polyomavirus (JCPyV), hepatitis A virus (HAV), and noroviruses (HuNoV GI and GII). Escherichia coli and physicochemical parameters (salinity, temperature, pH and dissolved oxygen) were also evaluated. From the 132 samples analyzed, 55% were positive for HAdV, 51.5% for HAV, 7.5% for HuNoV GI, 4.5% for HuNoV GII, and 3% for JCPyV. E. coli levels ranged from 8 to 1325 CFU/100mL at all sites. The overall results highlight the problem of sewage discharge into coastal waters and confirm that there is no correlation between viral presence and bacterial contamination.


Assuntos
Monitoramento Ambiental/métodos , Água do Mar/química , Poluentes da Água/análise , Adenovírus Humanos/isolamento & purificação , Brasil , DNA Viral/análise , Escherichia coli/crescimento & desenvolvimento , Escherichia coli/isolamento & purificação , Vírus da Hepatite A/isolamento & purificação , Norovirus/isolamento & purificação , Água do Mar/microbiologia , Água do Mar/virologia , Esgotos/análise , Esgotos/estatística & dados numéricos , Poluição da Água/análise , Poluição da Água/estatística & dados numéricos
14.
AJNR Am J Neuroradiol ; 31(6): 1059-62, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20053803

RESUMO

BACKGROUND AND PURPOSE: Hippocampal abnormalities are known as highly epileptogenic precursor lesions in the general population, primarily manifesting as MTS. The purpose of this study was to evaluate the occurrence of hippocampal abnormalities on MR imaging in patients with TS to explore the possible underlying mechanisms of the abnormalities and to identify the relationship between an abnormal HF and epilepsy. MATERIALS AND METHODS: We studied MR images and clinical data from 31 patients with TS. The MR imaging protocol was identical for all patients and included tilted coronal images of their temporal lobes. The diagnosis of TSC was made according to established criteria. The HFs of the patients were evaluated from coronal images according to size, morphology, and signal intensity. The data were submitted to statistical analysis, and P values < or = .05 were considered significant. RESULTS: We found HF abnormalities in 5 patients. Four had typical MTS, and 1 had HIMAL. We found a positive correlation between childhood febrile seizures and MTS in patients with TS. We also confirmed that patients with altered hippocampi had a tendency to exhibit more cortical tubers. CONCLUSIONS: Analysis of this series of patients demonstrated the presence of HF abnormalities, mainly MTS in patients with TS. We also found that the occurrence of febrile seizures during the first year of life appears to be one of the determining factors for MTS development in these patients.


Assuntos
Epilepsia do Lobo Temporal/patologia , Hipocampo/anormalidades , Hipocampo/patologia , Imageamento por Ressonância Magnética , Esclerose Tuberosa/patologia , Adolescente , Adulto , Atrofia , Criança , Pré-Escolar , Eletroencefalografia , Feminino , Humanos , Lactente , Masculino , Convulsões Febris/patologia , Lobo Temporal/patologia , Adulto Jovem
15.
J Pathol ; 217(4): 516-23, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-18991334

RESUMO

Amplification of the 11q13 region is a prevalent genetic alteration in head and neck squamous cell carcinoma (HNSCC). We investigated the clinical significance of cortactin (CTTN) and cyclin D1 (CCND1) amplification in both malignant transformation and tumour progression. CTTN and CCND1 amplification was analysed by differential and real-time PCR in a prospective series of laryngeal/pharyngeal carcinomas and archival premalignant tissues. CTTN mRNA and protein expression were respectively determined by real-time RT-PCR and immunohistochemistry, and correlated with gene status. Molecular alterations were associated with clinicopathological parameters and disease outcome. CTTN and CCND1 amplifications were respectively found in 75 (37%) and 90 (45%) tumours. Both correlated with advanced disease; however, only CTTN amplification was associated with recurrence and reduced disease-specific survival (p = 0.0022). Strikingly, CTTN amplification differentially influenced survival depending on tumour site (p = 0.0001 larynx versus p = 0.68 pharynx) and was an independent predictor of reduced survival in the larynx (p = 0.04). CCND1 amplification was detected in early tumourigenesis and increased with the severity of dysplasia. Importantly, CTTN amplification was only found in high-grade dysplasias that progressed to invasive carcinoma. CTTN gene status strongly correlated with mRNA and protein expression. Furthermore, CTTN overexpression correlated significantly with reduced disease-specific survival (p = 0.018). Taken together, these data indicate that CTTN may serve as a valuable biomarker to identify patients with laryngeal tumours at high risk of recurrence and poor outcome.


Assuntos
Carcinoma de Células Escamosas/genética , Cromossomos Humanos Par 11 , Cortactina/genética , Regulação Neoplásica da Expressão Gênica , Neoplasias de Cabeça e Pescoço/genética , Recidiva Local de Neoplasia/genética , Biomarcadores Tumorais/análise , Carcinoma de Células Escamosas/mortalidade , Carcinoma de Células Escamosas/patologia , Cortactina/análise , Cortactina/metabolismo , Ciclina D1/análise , Ciclina D1/genética , Ciclina D1/metabolismo , Feminino , Amplificação de Genes , Neoplasias de Cabeça e Pescoço/mortalidade , Neoplasias de Cabeça e Pescoço/patologia , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia/mortalidade , Recidiva Local de Neoplasia/patologia , Estadiamento de Neoplasias , Prognóstico , Modelos de Riscos Proporcionais , RNA Mensageiro/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Taxa de Sobrevida
16.
J Cardiovasc Surg (Torino) ; 49(2): 167-77, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18431336

RESUMO

The prevalence of peripheral arterial disease (PAD) continues to rise in an ever ageing society and consumes a significant part of health resources. Percutaneous revascularization has revolutionized the treatment of lower extremity peripheral vascular disease over the past 10 years. Additionally, novel devices have allowed improved endovascular treatment of femoropopliteal as well as infrapopliteal disease. Although percutaneous transluminal angioplasty (PTA) can be an effective modality for focal lesions in the iliac arteries, the results for complex infra-inguinal arterial disease have been disappointing. One class of new technology has concentrated on debulking the plaque, while others focus to improve safety (distal embolic protection devices) or are directed to specific clinical challenges such as chronic total occlusions. However, the lack of uniform performance criteria and reporting standards for these and other devices has resulted in heterogeneous study end points, making comparative efficacy difficult. Here we review the current data for atherectomy and atheroablative technologies as well as other adjunctive devices in the treatment of lower extremity peripheral arterial disease.


Assuntos
Arteriopatias Oclusivas/terapia , Aterectomia , Doenças Vasculares Periféricas/terapia , Angioplastia com Balão , Aterectomia/instrumentação , Filtração/instrumentação , Humanos , Perna (Membro)/irrigação sanguínea , Trombectomia/instrumentação , Tromboembolia
17.
Ann N Y Acad Sci ; 1108: 540-53, 2007 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-17894019

RESUMO

It has been shown that endothelial cell (EC) activation and tissue factor (TF) upregulation in EC and monocytes by antiphospholipid antibodies (aPL Abs) leads to a prothrombotic state and involves translocation of nuclear factor-kappa B (NF-kappaB). Here we examined the effects of an NF-kappaB inhibitor on aPL-induced thrombosis, TF activity, and EC in vivo. We treated CD1 mice with IgG from a patient with antiphospholipid syndrome (IgG-APS) or with control IgG (IgG-NHS). The adhesion of leukocytes (number of white blood cells) to EC in cremaster muscle (as an indication of EC activation) as well as the size of an induced thrombus in the femoral vein of the mice were examined. Some mice in each group were infused with 10 microM MG132 (an inhibitor of NF-kappaB). TF activity was determined using a chromogenic assay in homogenates of carotid arteries and in peritoneal cells of mice. In vivo, IgG-APS increased significantly the number of white blood cells adhering to ECs (4.7 +/- 2.2) when compared to control mice (1.5 +/- 0.8), and these effects were significantly reduced when mice were pretreated with MG132 (0.8 +/- 0.2). IgG-APS increased significantly the thrombus size and MG132 inhibited that effect (93%). Treatment of the mice with IgG-APS also induced significantly increased TF function in peritoneal cells and in homogenates of carotid arteries. Pretreatment of the mice with MG132 abrogated those effects significantly. Mice injected with IgG-APS or with IgM-APS with or without the inhibitor had medium-high titers of anticardiolipin antibodies in serum at the time of the surgical procedures. The data show that prothrombotic and proinflammatory properties of IgG-APS and IgM-APS are downregulated in vivo by an NF-kappaB inhibitor. These findings may be important in designing new modalities of targeted therapies to treat thrombosis in patients with APS.


Assuntos
Anticorpos Antifosfolipídeos/metabolismo , Adesão Celular/efeitos dos fármacos , Leupeptinas/farmacologia , NF-kappa B/antagonistas & inibidores , Trombose/metabolismo , Animais , Síndrome Antifosfolipídica/sangue , Síndrome Antifosfolipídica/imunologia , Células Endoteliais/efeitos dos fármacos , Células Endoteliais/metabolismo , Humanos , Masculino , Camundongos , Pessoa de Meia-Idade , NF-kappa B/efeitos dos fármacos , Tromboplastina/efeitos dos fármacos
18.
J Appl Microbiol ; 103(4): 864-70, 2007 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-17897188

RESUMO

AIMS: The aim of this study was to investigate the behaviour of a Serratia marcescens-Kluyveromyces fragilis mixed culture in whey, with the objective of proteases production and organic waste reduction. METHODS AND RESULTS: Discontinuous aerobic fermentations in whey were carried out using individual pure cultures and mixed cultures of S. marcescens and K. fragilis. Cell growth, protease production, lactose and proteins consumption and COD/TOC reduction were monitored. Lactose and protein content of the fermenting medium was almost depleted in the mixed cultures, achieving a reduction in the organic content much higher than in both pure cultures. Interestingly, proteolytic activity in the mixed cultures was similar to that obtained for S. marcescens in pure culture. In addition, protease stability was increased in the mixed cultures. Kinetic models were developed fitting well with the experimental results. CONCLUSIONS: Mixed cultures were found to maintain the achievements of each individual fermentation, yielding a high and stable production of proteases and a significant reduction of COD/TOC. SIGNIFICANCE AND IMPACT OF THE STUDY: Mixed cultures tested in this work have shown a synergistic effect with possible industrial applications. These results lead to a gain in the chain value for enzyme production with an environmentally friendly operation.


Assuntos
Microbiologia de Alimentos , Kluyveromyces/fisiologia , Leite/microbiologia , Peptídeo Hidrolases/biossíntese , Serratia marcescens/fisiologia , Animais , Reatores Biológicos , Meios de Cultura , Fermentação , Kluyveromyces/crescimento & desenvolvimento , Lactose/metabolismo , Leite/metabolismo , Proteínas do Leite/metabolismo , Modelos Biológicos , Serratia marcescens/crescimento & desenvolvimento
19.
J Hand Surg Br ; 31(4): 453-4, 2006 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-16618524

RESUMO

Avascular necrosis of the pisiform bone is a very rare condition. This paper reports a case and discusses this pathology and its diagnosis.


Assuntos
Isquemia/patologia , Isquemia/cirurgia , Pisciforme/patologia , Pisciforme/cirurgia , Feminino , Humanos , Isquemia/diagnóstico por imagem , Imageamento por Ressonância Magnética , Pessoa de Meia-Idade , Necrose , Dor , Pisciforme/diagnóstico por imagem , Radiografia , Resultado do Tratamento , Ulna
20.
Neurocirugia (Astur) ; 15(3): 290-3, 2004 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-15239016

RESUMO

Intradural spinal teratoma is a very rare entity, more prevalent in childhood, that may associate with dysraphic defects. The authors report a 46 years old man with a cauda equina syndrome and an L1-2 tumor. An L1-2 laminectomy was performed and a mass was resected at the base of the filum terminale, which was in contact with the conus medullaris. Histopathological diagnosis was of cystic mature teratoma. Spinal teratomas may be found anywhere along the spine, but are more frequent in the cauda equina. We discuss the origin of these tumors and review the literature concerning these lesions.


Assuntos
Cauda Equina/patologia , Neoplasias da Coluna Vertebral/patologia , Teratoma/patologia , Cauda Equina/cirurgia , Humanos , Laminectomia/métodos , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Neoplasias da Coluna Vertebral/cirurgia , Teratoma/cirurgia
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