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1.
J Biophotonics ; 13(1): e201900224, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31568652

RESUMO

Dental caries usually occurs at interproximal and occlusal surfaces. The purpose of the present study was to determine if characteristic spectral factors extracted from autofluorescence (AF) spectra are informative regarding caries detection and the determination of caries stage as compared with DIAGNOdent results. AF spectra were obtained from caries lesions of different severities at two locations using a 405 nm laser. Three spectral factors, that is, spectral slope at 550 to 600 nm, spectral area under the curve at 500 to 590 nm and two-peak ratio between 625 and 667 nm, were extracted. The values of three spectral factors linearly decreased as caries progressed. According to micro-CT images, conventional visual and tactile inspections of lesions under or overestimated (25%-65%) caries states, and brown or thickly stained layer on interproximal or occlusal surfaces, respectively, caused misclassifications of caries stage. Of the spectral factors examined, spectral slope and area under curve for interproximal and occlusal surfaces, respectively, were found to be significantly related to caries stage and showed least data overlap. For interproximal and occlusal surfaces, DIAGNOdent readings of different stages overlapped considerably though their mean values were significantly different regardless of stage.

2.
Braz Dent J ; 30(6): 569-576, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31800751

RESUMO

This study evaluated the influence of a retentive shoulder design to prevent early failure of three resin composite build-ups on molars. Ninety-six intact extracted human maxillary third molars were assigned to two groups (n=48) based on occlusal, buccal cusps only and all cusps reduction. The groups were divided into two subgroups: pin retained and non-pin retained build-ups resulting in four groups (n=24), according the cusps reduction: pin retained/partial, pin retained/complete, non-pin retained/partial, non-pin retained/complete. Occlusal reduction was 3 mm with a semi-lunar retentive shoulder of 3 mm and an axial wall height of 1.5 mm. Groups were restored using a microhybrid, flowable or titanium reinforced resin composite. Modified self-curing acrylic resin provisional crowns were fabricated, cemented with non-eugenol temporary cement and thermal cycled. An instron machine applied tension to the provisional crowns parallel to the long axis of the tooth until dislodgement. A three-way analysis of variance was conducted on the influence of the variables on the retention of the core build-up. Ninety-three build-ups were retained under tensile load, while three core build-ups without pins were dislodged. Three-way analysis of variance showed no statistically significant difference between core build-ups using a retentive shoulder and pin retained core build-ups when tested under tensile load. With the advent of adhesive systems, increased surface area and retentive shoulder design can provide a retentive core foundation to prevent early failures of core build-ups during indirect restoration fabrication which will contribute to the longevity of final restorations.


Assuntos
Retenção em Prótese Dentária , Ombro , Resinas Compostas , Coroas , Cimentos Dentários , Análise do Estresse Dentário , Humanos , Teste de Materiais , Cimentos de Resina
3.
Nanomaterials (Basel) ; 9(12)2019 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-31818019

RESUMO

BACKGROUND: Calcium phosphate-based compounds are used to treat dental hypersensitivity (DH). Their long-term clinical behaviour needs further research. This study compared the 24-week effectiveness of Teethmate Desensitizer (TD), a pure tetracalcium phosphate (TTCP) and dicalcium phosphate dihydrate (DCPD) powder/water, to that of Dentin Desensitizer (DD), and Bite & White ExSense (BWE), both of calcium phosphate crystallites. METHODS: A total of 105 subjects were selected. A random table was utilised to form three groups of 35 subjects. DH was evaluated using the evaporative sensitivity, tactile sensitivity tests, and the visual analogue scale (VAS) of pain. Response was recorded before the application of the materials (Pre-1), immediately after (Post-0), at 1 week (Post-1), 4 weeks (Post-2), 12 weeks (Post-3) and 24 weeks (Post-4). The non-parametric distribution was assessed with the Shapiro-Wilk statistical test. Intra-group differences for the six time points were evaluated with the Friedman statistical test and the Kruskal-Wallis test. RESULTS: All the materials decreased DH after 24 weeks in comparison to Pre-1. However, the TTCP/DCPD cement showed the greatest statistical efficiency. CONCLUSIONS: The significant decrease of VAS scores produced by TD in the long term suggest the material as the most reliable in the clinical relief of DH.

4.
J Clin Periodontol ; 46(12): 1205-1216, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31562774

RESUMO

AIM: To estimate gingivitis effects of a bioavailable gluconate chelated 0.454% stannous fluoride (SnF2 ) family of dentifrices in adult subjects versus positive (triclosan) and negative (NaF or MFP) controls when used ≤3 months. MATERIALS AND METHODS: A meta-analysis evaluated bioavailable gluconate chelated SnF2 dentifrices versus a negative or positive control for gingival bleeding. RESULTS: In 18 randomized controlled trials (RCTs) with 2,890 subjects assessing SnF2 paste versus a negative or positive control, the average number of bleeding sites was reduced by 51% and 31%, respectively. The average change (95% CI) in number of bleeding sites was -16.3 (-27.8, -4.9) versus the negative control and -3.6 (-5.4, -1.8) versus the positive control. Subjects with localized or generalized gingivitis had 3.7 times better odds (95% CI [2.8, 5.0]) of shifting to generally healthy using SnF2 versus a negative control and 2.8 times better odds (95% CI [2.1, 3.9]) of shifting to generally healthy using SnF2 versus a positive control. The individual study risk of bias was deemed to be low in all categories of bias. CONCLUSION: This meta-analysis demonstrates significant gingivitis benefits of bioavailable SnF2 dentifrices when used ≤3 months versus positive (triclosan) and negative (NaF or MFP) controls.


Assuntos
Dentifrícios , Gengivite , Adulto , Método Duplo-Cego , Gluconatos , Humanos , Índice Periodontal , Ensaios Clínicos Controlados Aleatórios como Assunto , Fluoreto de Sódio , Fluoretos de Estanho
5.
Am J Dent ; 32(4): 165-168, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31436935

RESUMO

PURPOSE: To evaluate the biomechanical behavior of monolithic ceramic crowns with functional elasticity gradient. METHODS: Using a CAD software, a lower molar received a full-crown preparation (1.5 mm occlusal and axial reduction). The monolithic crown was modeled with a resin cement layer of 0.1 mm. Four groups were distributed according to the full crown elastic modulus (E) :(a) Bioinspired crown with decreasing elastic modulus (from 90 to 30GPa); (b) Crown with increasing elastic modulus (from 30 to 90 GPa); (c) Rigid crown (90 GPa) and (d) Flexible crown (30 GPa). The model was exported to the analysis software and meshed into 385.240 tetrahedral elements and 696.310 nodes. Materials were considered isotropic, linearly elastic, and homogeneous, with ideal contacts. A 300-N load was applied at the occlusal surface and the base of the model was fixed in all directions. The results were required in maximum principal stress criterion. RESULTS: Crowns consisting of layers with increasing elastic modulus presented intermediate results between the rigid and flexible crowns. Compared to the flexible crown, the bioinspired crown showed acceptable stress distribution across the structure with lower stress concentration in the tooth. In dental crowns the multilayer structure with functional elasticity gradient modifies the stress distribution in the restoration, with promising results for bioinspired design. CLINICAL SIGNIFICANCE: The manufacturing of posterior crowns with functional elasticity gradient should be considered due to its promising results on the stress concentration behavior.


Assuntos
Coroas , Cimentos de Resina , Porcelana Dentária , Planejamento de Prótese Dentária , Análise do Estresse Dentário , Módulo de Elasticidade , Elasticidade , Teste de Materiais , Dente Molar , Estresse Mecânico
6.
J Prosthodont ; 28(6): 677-683, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31149753

RESUMO

PURPOSE: To investigate the effect of the location of a partial ferrule on 2 walls and the influence of ferrule height variations on remaining walls, fracture resistance, and failure mode of maxillary incisors endodontically treated and restored with fiber posts. MATERIALS AND METHODS: Sixty intact human maxillary central incisors were divided into 6 groups (n = 10): no ferrule (NF), 2-mm ferrule (CF2), absence of lingual wall and 1 proximal wall with remaining walls' height, 1 mm (IF1), 2 mm (IF2), 3 mm (IF3), and 4 mm (IF4). They were restored with a fiberglass post and composite resin core. Complete metal crowns were then cemented on all specimens. Completed specimens were subjected to thermal cycling (6000 cycles, 5°C/55°C) followed by the immediate testing of fracture resistance. After failure, the specimens were sectioned buccolingually and evaluated to identify the mode of failure. The data were analyzed with an ANOVA and the Student-Newman-Keuls multiple comparison tests (α = 0.05). RESULTS: An incomplete ferrule (IF2) with the lingual wall missing and 1 proximal wall had a fracture resistance of 494 ± 137 N, which was significantly less that of a complete ferrule (CF2) (707 ± 162 N, p = 0.002). An increase of 3 to 4 mm in height of the remaining walls had negligible influence on the fracture resistance: 514 ± 117 N (IF3), 557 ± 177 N (IF4). Partial decementation was observed in all specimens of IF3 and IF4, in 9 of IF1 and IF2, in 6 in NF, and in 3 in CF2. Root fractures occurred in 7 specimens, 4 in CF2, 1 in IF1, 1 in IF2, and 1 in IF4. Cracks under the cemento-enamel junction occurred in 4 specimens (NF and IF1), in 5 (IF2 and IF3), and in 6 (CF2 and IF4) and were considered catastrophic failures. CONCLUSIONS: Specimens with a complete ferrule of 2 mm height were more resistant to fracture than specimens with 2 mm height and absence of the lingual wall and 1 proximal wall. A 3 or 4 mm wall height increase was associated with an insignificant increase in fracture resistance and cannot compensate for the absence of the lingual wall and 1 proximal wall.


Assuntos
Técnica para Retentor Intrarradicular , Fraturas dos Dentes , Dente não Vital , Resinas Compostas , Coroas , Falha de Restauração Dentária , Análise do Estresse Dentário , Humanos , Incisivo
7.
Am J Dent ; 32(2): 55-60, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31094138

RESUMO

PURPOSE: To examine the influence of different bulk and block composite and flowable and glass-ionomer material combinations in a multi-layer technique and in a unique technique, in deep Class I dental restorations. METHODS: 3D CAD of the sound tooth were built-up from a CT scan dataset using reverse engineering techniques. Four restored tooth models with Class I cavity were virtually created from a CAD model of a sound tooth. 3D-finite element (FE) models were created and analyzed starting from CAD models. Model A with flowable resin composite restoring the lower layer and bulk-fill resin composite restoring the upper layer, model B with glass-ionomer cement (GIC) restoring the lower layer and bulk-fill resin composite restoring the upper layer, model C with block composite as the only restoring material and model D with bulk-fill resin composite as the only restoring material. Polymerization shrinkage was simulated with the thermal expansion approach. Physiologic masticatory loads were applied in combination with shrinkage effect. Nodal displacements on the lower surfaces of FE models were constrained in all directions. Static linear analyses were carried out. The maximum normal stress criterion was used to assess the influence of each factor. RESULTS: Considering direct restoring techniques, models A, B and D exhibited a high stress gradient at the tooth/restorative material interface. Models A and D showed a similar stress trend along the cavity wall where a similar stress trend was recorded in the dentin and enamel. Model B showed a similar stress trend along enamel/restoration interface but a very low stress gradient along the dentin/restoration interface. Model C with a restoring block composite material showed a better response, with the lowest stress gradient at the dentin, filling block composite and enamel sides. CLINICAL SIGNIFICANCE: Bulk resin-based composite materials applied in a multilayer technique to deep and large Class I cavities produced adverse stress distributions versus block resin composite. Polymerization shrinkage and loading determined high stress levels in deep Class I cavities with bulk multi-layer restorations, while its impact on adhesion in block composite restorations was insignificant.


Assuntos
Resinas Compostas , Restauração Dentária Permanente , Preparo da Cavidade Dentária , Análise do Estresse Dentário , Análise de Elementos Finitos , Teste de Materiais , Polimerização , Estresse Mecânico
8.
Am J Dent ; 32(2): 74-80, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31094141

RESUMO

PURPOSE: This prospectively designed, non-validated in vivo diagnostic study compared the results of visual examination, digital bitewing (BW) radiography and near-infrared light transillumination ( NIR-LT, DIAGNOcam) on proximal caries detection in posterior teeth. METHODS: A total of 203 subjects (122 men:81 women; mean age, 23.0 years) were included. All subjects were visually examined according to the standards by the World Health Organization and the International Caries Detection and Assessment System. In addition, digital BW radiographs were performed. NIR-LT images were captured from all posterior teeth. All BWs and NIR-LT images were blindly evaluated for the presence of enamel caries lesions ( ECLs) and dentin caries lesions ( DCLs). No histological validation was performed due to the impossibility to investigate healthy surfaces and non-cavitated caries lesions invasively. The statistical analysis included both descriptive and exploratory data evaluations. RESULTS: The diagnostic outcome differed for each method. Compared with BW radiography ( 8.0 surfaces) and NIR-LT (10.5 surfaces), visual examination revealed the fewest caries-related findings (4.2 surfaces). BW radiography or NIR-LT detected either 86.2% or 89.6% of all ECLs/DCLs in posterior teeth alone. When combining visual examination with NIR-LT, 70.9% of all ECLs/DCLs were similarly detected; when visual examination and BW radiography were combined, this proportion was lower ( 52.6%). CLINICAL SIGNIFICANCE: This study confirmed that visual examination alone led to an underestimation of the caries burden on proximal sites in posterior teeth. The novel near-infrared light transillumination might be a useful additional caries detection and diagnostic method.


Assuntos
Cárie Dentária , Transiluminação , Adulto , Cárie Dentária/diagnóstico por imagem , Feminino , Humanos , Masculino , Radiografia Interproximal , Radiografia Dentária Digital , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Adulto Jovem
9.
J Oral Implantol ; 45(4): 267-273, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31008689

RESUMO

When dental implants become infected, the progression of the disease is rapid. Commercially available dental implant surfaces can be easily contaminated, resulting in rapid progression of peri-mucositis and peri-implantitis. The aim of this study was to evaluate, in vitro, the pattern of doxycycline release from by dental implants with titanium nanotube surface (DINS) at different pHs to examine novel drug loading and chemical coating techniques. Nine DINS were loaded with doxycycline and subsequently coated with polylactic-co-glycolic acid (PLGA). High-performance liquid chromatography (HPLC) was used to measure the amounts of released doxycycline in a 30-day period. Cytotoxicity of the DINS was evaluated by an assay using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT). The results showed that the experimental DINS coated with doxycycline and PLGA showed a mean drug release during the experimental period for the groups: pH 7.4 (8.39 µg/mL), pH 6.4 (8.63 µg/mL). The pH 5.4 (15.18 µl/mL) doxycycline release from DINS was faster at pH 5.4 than those at pHs 6.4 and 7.4 (P = .0031 and .0034, respectively). This new surface treatment of dental implants with titanium nanotubes and subsequent drug loading demonstrated biocompatibility and sustained doxycycline release over a 30-day period. Additional studies are needed in order to adopt a stable drug release at neutral pH environment while warranting a constant drug release in an acidic pH environment.


Assuntos
Antibacterianos , Implantes Dentários , Doxiciclina , Nanotubos , Antibacterianos/farmacocinética , Doxiciclina/farmacocinética , Glicolatos , Glicóis , Copolímero de Ácido Poliláctico e Ácido Poliglicólico
10.
Med. oral patol. oral cir. bucal (Internet) ; 24(2): e156-e164, mar. 2019. tab, ilus, graf
Artigo em Inglês | IBECS | ID: ibc-180638

RESUMO

Background: The aim was to evaluate the effect of silver loaded nanoparticles (NPs) application on the tribo¬scopic, crystallographic and viscoelastic properties of demineralized dentin. Polymethylmetacrylate-based NPs and Ag loaded NPs were applied on demineralized dentin. Material and Methods: Treated and untreated surfaces were probed by a nanoindenter to test viscoelasticity, and by atomic force microscopy to test nanoroughness and collagen fibril diameter. X-ray diffraction and transmission electron microscopy through selected area diffraction and bright-field imaging were also used. Results: Dentin treated with Ag-NPs attained the lowest complex modulus, and the highest tan delta values after 7 days of storage. Dentin treated with undoped-NPs achieved the lowest nanoroughness and the greatest collagen bandwidths among groups. Crystals were identified as hydroxyapatite with the highest crystallographic maturity and crystallite size in dentin treated with undoped-NPs. Texture increased in all samples from 24 h to 7 d, except in dentin surfaces treated with Ag-NPs at 310 plane. Polyhedral, block-like, hexagonal or plate-like shaped apatite crystals constituted the bulk of minerals in dentin treated with Ag-NPs, after 7 d. Polyhedral or rounded/drop-like, and polymorphic in strata crystal apatite characterized the minerals when undoped-NPs were used, with more crystalline characteristics after 7 d than that found when Ag-NPs were applied. Ag-NPs application did not improve the mechanical performance of dentin and did not produce dentin remineralization. However, energy was dissipated through the dentin without showing stress concentration; contrary was occurring at dentin treated with undoped- NPs, that provoked bridge-like mineral deposits at the dentin surface. Conclusions: Ag-NPs application did not enhance the mechanical properties of cervical dentin, though the energy dissipation did not damage the dentin structure. Remineralization at dentin was not produced after Ag-NPs appli-cation, though improved crystallinity may lead to increase stability of the apatite that was generated at the dentin surface


No disponible


Assuntos
Humanos , Dentina/fisiopatologia , Nanopartículas Metálicas/uso terapêutico , Prata/uso terapêutico , Desmineralização do Dente/terapia , Remineralização Dentária/métodos , Calcificação Fisiológica/fisiologia
11.
Clin Oral Investig ; 23(1): 293-301, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29671053

RESUMO

OBJECTIVE: Visual differentiation of resin and tooth in a tooth cavity is not simple due to their highly similar shade. The purpose of the present study was to find any noninvasive method which can effectively differentiate resin from sound tooth in a resin-imbedded tooth for resin repair. MATERIALS AND METHODS: For the study, various resin products were imbedded into the cavity of sound tooth. By applying laser of different wavelengths, autofluorescence (AF) of sound tooth and resin products were obtained. Microhardness, X-ray radiograph, and DIAGNOdent were tested for each tooth, resin product, and resin-imbedded tooth. RESULTS: For the AF spectra obtained using the 405-nm wavelength, sound tooth has emission peak at 440-470 nm and near 490 nm. Sound tooth has several times higher microhardness than resin products regardless of position in tooth subsurface. Due to the difference of radiopaque fillers' composition and concentration, resin products have different brightness in the X-ray radiograph. DIAGNOdent readings for tooth and resin products were inconsistently different, and the difference of obtained values was slightly not to be applicable for the differentiation. CONCLUSION: Among the tested methods, with noninvasive treatment, AF spectrum by the 405-nm wavelength showed the apparent difference between resin and tooth. CLINICAL SIGNIFICANCE: For the resin repair in a resin-imbedded tooth cavity, AF spectrum produced by 405-nm wavelength could be a useful method in tracing the resin-tooth boundary if combined with conventional X-ray radiography.


Assuntos
Resinas Compostas/química , Restauração Dentária Permanente , Dente Pré-Molar , Fluorescência , Dureza , Humanos , Técnicas In Vitro , Lasers , Teste de Materiais , Dente Molar , Propriedades de Superfície , Raios X
12.
Stem Cells Dev ; 28(2): 141-150, 2019 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-30426846

RESUMO

In recent years, regenerative medicine has directed its interests onto the use of stem cells to heal human tissues. One specific class of cells that has been used in this field of research is mesenchymal stem cells (MSCs). Because of difficulties with the usage of whole stem cells, researchers have turned to an alternative, the secretome of the MSCs. In recent years, research has explored numerous aspects of the MSC secretome, especially the most promising aspect, exosomes. This review explores a variety of interests in exosomes including the classification and molecular composition of exosomes, mechanisms for exosome isolation, and the various biological functions of exosomes. As more is discovered about the exosomes, their different diagnostic and therapeutic uses in the medical field have also been explored. A new field attempting to exploit the exosomes in clinical practice is orthopedics. Although a significant deal of research has been carried out, even more is being discovered to allow utilization of the exosomes in clinical practice.

13.
Am J Dent ; 32(6): 276-280, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31920052

RESUMO

PURPOSE: To evaluate the antibacterial efficiency of silver-loaded gelatin sponges prepared from gelatin and silver diamine fluoride, Ag(NH3)2F. METHODS: A series of novel silver-loaded gelatin sponges were prepared from gelatin and silver diamine fluoride. They were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electronic microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). The antibacterial activities against one oral bacteria model S. mutans were evaluated using the agar disk diffusion method and biofilm-grown bacteria assay. For the purpose of comparison, a second series of silver-loaded sponges were prepared using silver nitrate (AgNO3) as the silver source. RESULTS: FT-IR, SEM and EDX results confirmed the successful preparation of silver-loaded gelatin sponges from both silver diamine fluoride and silver nitrate. Agar disk diffusion assays revealed that the antibacterial activity of silver-loaded sponges was strongly correlated with the silver content, and also depending on the silver source used in the preparation of sponges. Sustained inhibition of S. mutans in agar plates was observed for silver-loaded gelatin sponges containing about 5 wt% Ag or more. Biofilm assays showed different viabilities when treated with different formulations, with the viability of 11.4±3.1% for the sponges containing 5.30±1.18 wt% Ag prepared from silver diamine fluoride, and the viability of 15.4±3.1% for the sponges containing 29.13±10.34 wt% Ag prepared from silver nitrate. CLINICAL SIGNIFICANCE: The silver loading contents had a significant effect on the antibacterial activities of silver-loaded gelatin sponges prepared with silver diamine fluoride. In addition, silver diamine fluoride was a superior silver source to prepare antibacterial silver-loaded gelatin sponges when compared with silver nitrate.


Assuntos
Fluoretos Tópicos , Gelatina , Antibacterianos , Compostos de Amônio Quaternário , Compostos de Prata , Espectroscopia de Infravermelho com Transformada de Fourier
14.
Am J Dent ; 31(5): 243-248, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30346670

RESUMO

PURPOSE: To investigate the infiltration potential of different self-etch adhesives into natural non-cavitated proximal lesions and the effect of dehydration protocol on the infiltration of a self-etch adhesive. METHODS: 29 extracted molars and premolars with natural proximal lesions (ICDAS 1-2) were sectioned through the lesion providing two samples from each lesion. To compare the different adhesives, three groups of eight lesions were abraded with fine metallic strips and then etched with 37% H3PO4 acid for 120 seconds. All teeth were stained with rhodamine isothiocyanate. After drying with compressed air and ethanol application, lesions were infiltrated with Scotchbond Universal, Clearfil SE Protect or OneCoat 7 Universal for 180 seconds and then coated with a thin layer of flowable composite (Tetric Flow). To compare the effect of dehydration protocol on infiltration, two groups of nine paired lesions were pretreated as described above. One group was dried using compressed air alone and the second group was dried using compressed air and ethanol, both groups were then infiltrated with Scotchbond Universal then coated with a thin film of flowable composite. After light curing, un-encapsulated dye was bleached by immersion in hydrogen peroxide. Remaining lesion pores were stained with sodium fluorescein solution. Thin cuts of the teeth were observed with confocal microscopy and computer image analysis was performed (ImageJ). RESULTS: ANOVA and Duncan post-hoc tests showed no significant differences of the infiltrated area between the three adhesives (P= 0.835), no significant difference was found between the group dried with air compared to the one dried with air and ethanol. It can be concluded that the tested adhesives may be used for infiltration of natural lesions following the described pretreatment. CLINICAL SIGNIFICANCE: Enamel pretreatment with metallic strip and 37% H3PO4 acid promotes the infiltration of different adhesives into natural non-cavitated caries lesions.


Assuntos
Cárie Dentária , Cimentos Dentários , Resinas Compostas , Esmalte Dentário , Humanos , Teste de Materiais , Cimentos de Resina
15.
Am J Dent ; 31(5): 255-260, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30346672

RESUMO

PURPOSE: To compare a new technique of non-invasive proximal adhesive restoration (NIPAR) to the infiltration concept technique (ICON). METHODS: Extracted human posterior teeth with non-cavitated proximal carious lesions (ICDAS code 1-2) were cut vertically to obtain two symmetrical lesions. Group 1 (NIPAR):Half of the paired lesions surfaces (n=13) were abraded with metallic strips and etched with 37% H3PO4 for 120 seconds. Group 2 (ICON):The other half of the paired lesions' surfaces (n=13) were etched with 15% HCl gel for 120 seconds. All samples were then stained with rhodamine isothiocyanate (RITC). After ethanol drying and isolation of the cut surface, Group 1 samples were infiltrated with Scotchbond Universal for 180 seconds and coated with a thin film of Tetric flow. Group 2 samples were infiltrated with ICON infiltrant following manufacturer's instructions. After light curing, unbound rhodamine was bleached by immersion in 30% hydrogen peroxide for 12 hours. Remaining lesion pores were stained with sodium fluorescein solution. Samples were observed with confocal microscopy (CLSM) and the percentage of infiltration (area of resin infiltration/area of total demineralization ×100) was calculated using ImageJ. RESULTS: 11 samples out of 13 showed larger infiltrated area of the lesions in Group 1 (NIPAR) compared to Group 2 (ICON). Statistical analysis revealed a significant difference between the two groups (P< 0.05). Within the limitations of this study, NIPAR allowed for better infiltration of non-cavitated proximal carious lesions when compared to ICON. CLINICAL SIGNIFICANCE: The combination of infiltration and sealing using non-invasive proximal adhesive restoration (NIPAR) offers a suitable non-invasive treatment option for non-cavitated proximal lesions combining the advantages of sealing and infiltration.


Assuntos
Ataque Ácido Dentário , Cárie Dentária , Cimentos Dentários , Esmalte Dentário , Humanos , Dente Molar
16.
Am J Dent ; 31(5): 261-266, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30346673

RESUMO

PURPOSE: To evaluate the longevity and factors associated with failure of primary teeth restorations placed in high caries-risk children. METHODS: The sample was comprised of children treated in a University Dental Service. Patients' records were screened retrospectively to determine whether they had received restorative treatment in primary teeth presenting cavitated caries lesions. Kaplan-Meier estimator and Multivariate Cox regression analysis with shared frailty were used to assess restorations' survival and factors associated with failure, respectively. RESULTS: 123 high caries-risk children (10.3±4 DMF-T) with 316 restorations were analyzed. The 3-year survival reached 53.4% (AFR=18.8%). Restorations placed without rubber dam (P= 0.04), over selective caries removal (P= 0.03), with calcium hydroxide liner (P< 0.01) and glass-ionomer cement (P= 0.04) presented lower survival rates. Caries-controlled patients presented significantly (P= 0.03) higher rates of restoration survival (77.7%) than caries-active patients (49.9%). The adjusted model showed that restorations placed in teeth after selective caries removal showed 3.41 times higher risk of failure compared with restorations over complete caries removal (95%CI:1.37-8.46). CLINICAL SIGNIFICANCE: Adhesive restorations placed in high-caries experience patients have limited survival rates. Some treatment-related factors may influence the performance of these restorations. A strict preventive regimen to control dental caries activity must be followed in order to increase the restoration survival.


Assuntos
Resinas Compostas , Cárie Dentária , Restauração Dentária Permanente , Criança , Falha de Restauração Dentária , Cimentos de Ionômeros de Vidro , Humanos , Estudos Retrospectivos , Dente Decíduo
17.
Am J Dent ; 31(5): 272-276, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30346675

RESUMO

PURPOSE: To assess the clinical ability of marginal detection of different intraoral optical scanning (IOS) systems. METHODS: The Ethics Committee of the University of Siena, Italy approved the research project. Thirty patients in need of an onlay/inlay with supra-gingival margins were included and randomly divided in three groups of 10 (3× n=10) according to the IOS for chairside capturing:(A) GC-Europe (Aadva); (B) True-Definition-TD; (C) Trios. A total of 1 scans from each IOS test group (A-C), were obtained clinically and stored as STL-files. In addition, corresponding conventional impressions were taken for all 30 patients, poured with stone, and then processed by a laboratory scanner (Aadva), serving as controls. All 60 STL-files were imported to the Exocad platform for analysis. The horizontal distance between each preparation margin and the adjacent tooth was measured using the ruler tool in the software. The distance at which the detection of the margin started to become visibly unclear was recorded for the horizontal distances. Data was processed statistically by one-way ANOVA (P> 0.05). RESULTS: No statistically significant inter-test group differences could be identified (IOS A-C). The minimum distance from which a clear margin was visible, was 4.5 (SD 0.1) mm for all images, regardless of which IOS was used. Under these experimental clinical conditions, all tested IOS performed similarly. In contrast, all margins of the controls were clearly visible. CLINICAL SIGNIFICANCE: None of the tested intraoral scanning systems in this study were capable of recording a clear impression when the cervical margin for a posterior partial crown was located at a distance of less than 0.5 mm from the interproximal neighbor.


Assuntos
Projeto Auxiliado por Computador , Técnica de Moldagem Odontológica , Humanos , Projetos Piloto
18.
J Immunol Res ; 2018: 1290814, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30246031

RESUMO

Background: The HIN-200 family genes in humans have been linked to several autoimmune diseases-particularly to systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Recently, its human counterpart gene cluster, the Ifi200 family in mice, has been linked to spontaneous arthritis disease (SAD). However, many immune-mediated diseases (including RA and SLE) show gender difference. Understanding whether or not and how these genes play a role in sex difference in immune-mediated diseases is essential for diagnosis/treatment. Methods: This study takes advantage of the whole genome gene expression profiles of recombinant inbred (RI) strain populations from female and male mice to analyze potential sex differences in a variety of genes in disease pathways. Expression levels and regulatory QTL of Ifi200 family genes between female and male mice were first examined in a large mouse population, including RI strains derived from C57BL/6J, DBA/2J (BXD), and classic inbred strains. Sex similarities and differences were then analyzed for correlations with gene expression levels between genes in the Ifi200 family and four selected gene sets: known immune Ifi200 pathway-related genes, lupus-relevant genes, osteoarthritis- (OA-) and RA-relevant genes, and sex hormone-related genes. Results: The expression level of Ifi202b showed the most sex difference in correlation with known immune-related genes (the P value for Ifi202b is 0.0004). Ifi202b also showed gender difference in correlation with selected sex hormone genes, with a P value of 0.0243. When comparing coexpression levels between Ifi200 genes and lupus-relevant genes, Ifi203 and Ifi205 showed significant sex difference (P values: 0.0303 and 0.002, resp.). Furthermore, several key genes (e.g., Csf1r, Ifnb1, IL-20, IL-22, IL-24, Jhdm1d, Csf1r, Ifnb1, IL-20, IL-22, IL-24, and Tgfb2 that regulate sex differences in immune diseases) were discovered. Conclusions: Different genes in the Ifi200 family play different roles in sex difference among dissimilar pathways of these four gene groups.


Assuntos
Doenças Autoimunes/genética , Peptídeos e Proteínas de Sinalização Intracelular/genética , Proteínas Nucleares/genética , Animais , Feminino , Genoma/genética , Hormônios Esteroides Gonadais/genética , Humanos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos DBA , Sexo , Transcriptoma
19.
J Pharm Sci ; 107(12): 3134-3142, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30201483

RESUMO

It is important to address the periodontitis-associated bacteria in the residual subgingival plaque after scaling and root planing to successfully treat periodontitis. In this study, we explored the possibility of exploiting the ion pairing/complexation of minocycline, Ca2+, and sulfate/sulfonate-bearing biopolymers to develop an intrapocket delivery system of minocycline as an adjunct to scaling and root planing. Minocycline-calcium-dextran sulfate complex microparticles were synthesized from minocycline, CaCl2, and dextran sulfate. They were characterized using Fourier-transform infrared spectroscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. An in vitro release study was conducted to evaluate the release kinetics of minocycline from these microparticles. Agar disk diffusion assays and biofilm-grown bacteria assays were used to assess antibacterial capability. High loading efficiency (96.98% ± 0.12%) and high loading content (44.69% ± 0.03%) for minocycline were observed for these complex microparticles. Mino-Ca-DS microparticles achieved sustained release of minocycline for at least 9 days at pH 7.4 and 18 days at pH 6.4 in phosphate-buffered saline, respectively. They also demonstrated potent antimicrobial effects against Streptococcus mutans and Aggregatibacter actinomycetemcomitans in agar disk diffusion and biofilm assays. These results suggested that the ion pairing/complexation of minocycline, Ca2+, and sulfonate/sulfate-bearing biopolymers can be exploited to develop complex microparticles as local delivery systems for periodontitis treatment.


Assuntos
Antibacterianos/administração & dosagem , Cálcio/química , Preparações de Ação Retardada/química , Sulfato de Dextrana/química , Minociclina/administração & dosagem , Aggregatibacter actinomycetemcomitans/efeitos dos fármacos , Aggregatibacter actinomycetemcomitans/fisiologia , Antibacterianos/química , Antibacterianos/farmacologia , Biofilmes/efeitos dos fármacos , Linhagem Celular , Liberação Controlada de Fármacos , Humanos , Minociclina/química , Minociclina/farmacologia , Infecções por Pasteurellaceae/tratamento farmacológico , Periodontite/tratamento farmacológico , Streptococcus mutans/efeitos dos fármacos , Streptococcus mutans/fisiologia
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