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Int J Mol Sci ; 19(10)2018 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-30287737


Sipunculus nudus, an old marine species, has great potential for use as functional seafood due to its various bioactivities. Its potential antithrombotic activity pushed us to isolate the bio-active components bio-guided by tracking fibrinolytic activity. As a result, a novel protease named as SK (the kinase obtained from S. nudus) was obtained, which possessed a molecular weight of 28,003.67 Da and 15 N-terminal amino acid sequences of PFPVPDPFVWDTSFQ. SK exerted inhibitory effects on thrombus formation through improving the coagulation system with dose-effect relationship within a certain range. Furthermore, in most cases SK got obviously better effect than that of urokinase. With the help of untargeted mass spectrometry-based metabolomics profiling, arachidonic acid, sphingolipid, and nicotinate and nicotinamide mechanism pathways were found to be important pathways. They revealed that the effect mechanism of SK on common carotid arterial thrombosis induced by FeCl3 was achieved by inhibiting vessel contraction, platelet aggregation, adhesion, and release, correcting endothelial cell dysfunction and retarding process of thrombus formation. This study demonstrated SK was a promising thrombolytic agent on the basis of its comprehensive activities on thrombosis, and it should get further exploitation and utilization.

Trombose das Artérias Carótidas/tratamento farmacológico , Fibrinolíticos/metabolismo , Proteínas de Helminto/metabolismo , Nematoides/enzimologia , Peptídeo Hidrolases/metabolismo , Animais , Fibrinolíticos/química , Fibrinolíticos/uso terapêutico , Proteínas de Helminto/química , Proteínas de Helminto/uso terapêutico , Masculino , Peptídeo Hidrolases/química , Peptídeo Hidrolases/uso terapêutico , Ratos , Ratos Sprague-Dawley
Zhongguo Zhong Yao Za Zhi ; 39(7): 1285-9, 2014 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-25011270


OBJECTIVE: To establish a method for studying efficacious materials of traditional Chinese medicines from an overall perspective. METHOD: Carthamus tinctorius was taken the example. Its major components were depleted by preparing liquid chromatography. Afterwards, the samples with major components depleted were evaluated for their antioxidant effect, so as to compare and analyze the major efficacious materials of C. tinctorius with antioxidant activity and the contributions. RESULT: Seven major components were depleted from C. tinctorius samples, and six of them were identified with MS data and control comparison. After all of the samples including depleted materials are compared and evaluated for their antioxidant effect, the findings showed that hydroxysafflor yellow A, anhydrosafflor yellow B and 6-hydroxykaempferol-3, 6-di-O-glucoside-7-O-glucuronide were the major efficacious materials. CONCLUSION: This study explored a novel and effective method for studying efficacious materials of traditional Chinese medicines. Through this method, we could explain the direct and indirect contributions of different components to the efficacy of traditional Chinese medicines, and make the efficacious material expression of traditional Chinese medicines clearer.

Antioxidantes/química , Carthamus tinctorius/química , Medicamentos de Ervas Chinesas/química , Álcalis/química , Cromatografia Líquida de Alta Pressão , Glucosídeos/química , Espectrometria de Massas
Zhongguo Zhong Yao Za Zhi ; 38(24): 4214-9, 2013 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-24791519


Coptidis Rhizoma-Euodiae Fructus has been widely used for the treatment of digestive diseases since Song Dynasty, and therapeutic efficacy is very obvious. Modern research found that alkaloids are the main bio-active constituents, and some of their contents have striking difference after compatibility of the two herbs. The Chinese medicine pair (CMP) has extensive biological activities, such as the effect of gastrointestinal effect, anti-tumor, lowering the blood pressure and blood fat and so on. And some action mechanism of CMP also got partial demonstration. This paper mainly summarized the bio-active constituents, compatibility effects, action mechanism and clinical applications of the CMP, which can provide a basis for further research and development of the CMP.

Medicamentos de Ervas Chinesas , Evodia/química , Medicina Tradicional Chinesa/métodos , Animais , Interações de Medicamentos , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Humanos