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1.
Food Chem ; 366: 130576, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34348222

RESUMO

Cinnamon oil is obtained by steam distillation from cinnamon leaves and is usually considered highly cost-effective compared to bark oil, however, which results in tons of waste cinnamon leaves (WCL) discarded annually. By using MS/MS molecular networking (MN) assisted profiling, six main chemical diversities including flavonols and flavones, phenolic acids, lactones, terpenoids, phenylpropanoids and flavanols were rapid revealed from WCL aqueous extract. 101 compounds were tentatively identified by assigning their MS/MS fragments within typical pathways under ESI-MS/MS dissociation. The featured phenolic acids, terpenoids and their glycosides in cinnamon species were recognized as the main constituents of WCL. The hydrophilic lactones, lignans and flavanols were reported for the first time in cinnamon leaves. Furthermore, ABTS and FRAP assays integrated with MN analysis were conducted to uncover an antioxidant fraction, from which 40 potential antioxidant compounds were rapidly annotated. This fundamental information will help expand the utilization of WCL from cinnamon oil industry.


Assuntos
Antioxidantes , Cinnamomum zeylanicum , Cromatografia Líquida , Extratos Vegetais , Espectrometria de Massas em Tandem
2.
Biochem Pharmacol ; 194: 114798, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34678227

RESUMO

Drug affinity responsive target stability (DARTS) is a novel target discovery approach and is particularly adept at screening small molecule (SM) targets without requiring any structural modifications. The DARTS method is capable of revealing drug-target interactions from cells or tissues by tracking changes in the stability of proteins acting as receptors of bioactive SMs. Due to its simple operation and high efficiency, the DARTS method has been applied to uncover the drug-action mechanism. This review summarized analytical principles, protocols, validation approaches, applications, and challenges involved in the DARTS method. Due to the innate advantages of the DARTS method, it is expected to be a powerful tool to accelerate SM target discovery, especially for bioactive natural products with unknown mechanisms.

3.
J Nat Med ; 75(4): 915-925, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34189715

RESUMO

Two phenylpropanoid-conjugated iridoids, deglucosyl gaertneroside (1) and morindoidin (2), were isolated from the leaves of Morinda morindoides (Rubiaceae) by activity-guided fractionation using an anti-malarial activity assay. The known related iridoids molucidin (3) and prismatomerin (4), two lignans, abscisic acid, two megastigmanes, and two flavonol glycosides were also identified. The structures of isolated compounds were elucidated using spectroscopic analysis. The isolated compounds were evaluated for anti-malarial activity against the chloroquine/mefloquine-sensitive strains of Plasmodium falciparum together with cytotoxicity against adult mouse brain cells. Potent anti-malarial activity of 3 and 4 (IC50 of 0.96 and 0.80 µM, CC50 of 1.02 and 0.88 µM, and SI of 1.06 and 1.10, respectively) was shown, while new iridoids 1 and 2 and pinoresinol (5) displayed moderate activity (IC50 of 40.9, 20.6, and 24.2 µM) without cytotoxicity (CC50 > 50 µM). These results indicate that 1-5 may be promising lead compounds for anti-malarial drugs. In addition, our results imply the necessity of the quality control of the extract of M. morindoides leaves based on the contents of 1-5 in terms of the safety and efficacy.


Assuntos
Antimaláricos , Morinda , Animais , Antimaláricos/farmacologia , Iridoides/farmacologia , Camundongos , Extratos Vegetais/farmacologia , Folhas de Planta
4.
J Ethnopharmacol ; 264: 113322, 2021 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-32871236

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: The genus Melastoma consists of approximately 100 species distributed widely in tropical and subtropical countries, and Melastoma species are often used for medicinal purposes, such as treatment for bleeding, diarrhea, diabetes, and gynecological tumors by local people, mostly in Southeast Asian countries. AIM OF THE REVIEW: The present review summarizes the traditional uses, phytochemistry and pharmacology of species belonging to Melastoma to suggest further research strategies and to facilitate the exploitation of the therapeutic potential of Melastoma species for the treatment of human disorders. MATERIALS AND METHODS: Information related to the traditional uses, phytochemistry and pharmacological activities was systematically collected by searching for the word "Melastoma" in electronic databases, including SciFinder, Web of Science, PubMed, and Google Scholar, from Apr. 1968 until Dec. 2019. RESULTS: A systematic literature survey revealed that Melastoma spp. are widely distributed in southern Asia to northern Oceania and the Pacific Islands and are traditionally used to treat bleeding, diarrhea, swelling, and gynecological tumors. Approximately 142 compounds, including flavonoids, tannins, phenylpropanoids, organic acids, terpenoids, and steroids, have been reported from Melastoma spp. Different extracts have been evaluated for their pharmacological activities, including anti-inflammatory, hemostatic, anticoagulant, cytotoxic, antibacterial, antioxidant, hepatoprotective, gastroprotective and hypoglycemic activities. CONCLUSIONS: Melastoma spp. are popularly used in Southeast Asian countries as effective herbs and are rich in flavonoids, tannins and organic acids with valuable medicinal properties. However, additional studies of the chemical constituents and the mechanism-based pharmacological activities of many members of Melastoma are still needed for developing new plant-derived drugs. In addition, studies on the clinical safety and efficacy of Melastoma are also needed.


Assuntos
Etnofarmacologia/métodos , Medicina Tradicional/métodos , Melastomataceae , Compostos Fitoquímicos/uso terapêutico , Extratos Vegetais/uso terapêutico , Animais , Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Anticoagulantes/isolamento & purificação , Anticoagulantes/farmacologia , Anticoagulantes/uso terapêutico , Etnofarmacologia/tendências , Flavonoides/isolamento & purificação , Flavonoides/farmacologia , Flavonoides/uso terapêutico , Humanos , Medicina Tradicional/tendências , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia
5.
Zhongguo Zhong Yao Za Zhi ; 45(16): 3908-3914, 2020 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-32893588

RESUMO

G-quadruplex DNA has become an important target for tumor therapy and anti-tumor development. Modern pharmacology has proved that Macleaya cordata has anti-inflammatory, antibacterial, anti-tumor and other pharmacological effects. Affinity ultrafiltration method can screen active ingredients from compounds rapidly, but G-quadruplex DNA ligands are difficult to dissociate, which is a key step in conventional ultrafiltration method. In this paper, the filtrates after ultrafiltration were determined by HPLC-MS in substitution. The peaks with 20% reduction of MS response from the incubation vs control were considered to be ligand components to G-quadruplex. Two of the peaks with the relative abundance above 30% were identified as sanguinarine(SAN) and chelerine(CHE). Their circular dichroism conformations further proved that SAN and CHE are active ligands of HT4. In addition, another two gradients with high relative abundance were identified as protopine(PRO) and allpcryprotopine(ALL). The binding rate of SAN, CHE, PRO and ALL was calculated according to the HPLC-MS results, and the results showed a consistency with that of the molecular docking method. The proposed method can be used to screen active components from mixture.


Assuntos
Quadruplex G , Ultrafiltração , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Ligantes , Espectrometria de Massas , Simulação de Acoplamento Molecular
6.
Zhongguo Zhong Yao Za Zhi ; 45(12): 2792-2799, 2020 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-32627452

RESUMO

Cinnamomum cassis is one of the commonly used traditional Chinese medicines in China. Its genuine producing areas distribute in Guangdong and Guangxi provinces. As an important edible herb and export variety of China, the quality control and internationalization of quality standards of C. cassis is extremely significant. In the recent years, with the development of the cinnamon industry, relevant academic research and the upgrade of the international standards, it is necessary to summarize the quality-related progress of C. cassis. In the present review, the germplasm resources, specific quality marker(Q-marker) and quality standards of C. cassis were summarized on the basis of published research during the last 10 years.


Assuntos
Cinnamomum aromaticum , Cinnamomum , China , Cinnamomum zeylanicum , Medicina Tradicional Chinesa
7.
Zhongguo Zhong Yao Za Zhi ; 44(19): 4152-4157, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31872692

RESUMO

Target discovery is the core of elucidating the mechanism of traditional Chinese medicine( TCM),and it is also the key to correlate the chemical composition and pharmacological action of TCM. The traditional target screening methods such as the activitybased probe profiling,affinity chromatography,and protein microarray are commonly used in the past,however,which are limited in TCM due to the complexity of small molecules existed in the herbal medicine. The label-free small molecule probe is a recently well-applied technology in the target discovery of natural products,which is characterized by discovering the small molecule-protein ligation without any structural modification at the ligands,and is therefore suitable to the complex chemical constituents in TCM. Furthermore,this method is conducted on the basis of proteome,which is advanced in the discovery of new or multiple target proteins of TCM. Owing to the potential of label-free probe in the target discovery of TCM,its analytical principle,application status,and general protocol were reviewed in this paper. The label-free probe technology is anticipated to accelerate the mechanism-uncovering of TCM.


Assuntos
Medicamentos de Ervas Chinesas , Medicina Tradicional Chinesa , Plantas Medicinais , Ligantes , Fitoterapia
8.
Zhongguo Zhong Yao Za Zhi ; 44(12): 2588-2593, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31359728

RESUMO

The PK-PD correlation models by using pharmacodynamics and pharmacokinetics were applied to study the material basis of Naomaitong,a clinical empirical prescription for the treatment of cerebral apoplexy,in inhibiting the death of PC12 nerve cells induced by Na_2S_2O_4 and Glu. In this experiment,PC12 cell death models induced by Na_2S_2O_4 and Glu were established respectively.With LDH lateral leakage and NO content as pharmacodynamic indexes,PK-PD model was established by SVM algorithm to evaluate the effective components of Naomaitong in inhibiting neural cell death. The results showed that the positive correlation of emodin methyl ether-8-O-ß-D-glucopyranoside,aloe emodin,chrysophanol,rhein,emodin,ginsenoside Rg1,ginsenoside Rc,3'-methoxypuerarin and ligustilide was significant,obviously improving the LDH release and NO content. The results indicated that the contribution of Radix Puerariae Lobatae Radix and Rhei Radix et Rhizoma in Naomaitong could protect the nerve cell death induced by Na_2S_2O_4 and Glu respectively. PK-PD model was used to screen the neuroprotective components in Naomaitong,revealing the possible pharmacodynamic material basis of Naomaitong in the treatment of cerebral ischemia injury.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Neurônios/citologia , Fármacos Neuroprotetores/farmacologia , Animais , Células PC12 , Ratos
9.
Nutrients ; 11(5)2019 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-31121888

RESUMO

The pharmacological properties of Eleutherococcus senticosus leaf have not been clarified although it is taken as a food item. In this study, the effects of water extract of Eleutherococcus senticosus leaves on memory function were investigated in normal mice. Oral administration of the extract for 17 days significantly enhanced object recognition memory. Compounds absorbed in blood and the brain after oral administration of the leaf extract were detected by LC-MS/MS analyses. Primarily detected compounds in plasma and the cerebral cortex were ciwujianoside C3, eleutheroside M, ciwujianoside B, and ciwujianoside A1. Pure compounds except for ciwujianoside A1 were administered orally for 17 days to normal mice. Ciwujianoside C3, eleutheroside M, and ciwujianoside B significantly enhanced object recognition memory. These results demonstrated that oral administration of the leaf extract of E. senticosus enhances memory function, and that active ingredients in the extract, such as ciwujianoside C3, eleutheroside M, and ciwujianoside B, were able to penetrate and work in the brain. Those three compounds as well as the leaf extract had dendrite extension activity against primary cultured cortical neurons. The effect might relate to memory enhancement.


Assuntos
Encéfalo/efeitos dos fármacos , Eleutherococcus/química , Memória/efeitos dos fármacos , Extratos Vegetais/farmacologia , Folhas de Planta/química , Animais , Barreira Hematoencefálica/metabolismo , Encéfalo/metabolismo , Células Cultivadas , Córtex Cerebral/química , Córtex Cerebral/embriologia , Córtex Cerebral/ultraestrutura , Dendritos/efeitos dos fármacos , Dendritos/fisiologia , Glicosídeos/análise , Glicosídeos/farmacocinética , Glicosídeos/farmacologia , Masculino , Camundongos , Extratos Vegetais/análise , Extratos Vegetais/farmacocinética , Saponinas/análise , Saponinas/farmacocinética , Saponinas/farmacologia
10.
Zhongguo Zhong Yao Za Zhi ; 44(2): 314-318, 2019 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-30989951

RESUMO

Nine compounds were isolated from chloroform fraction of Houttuynia cordata,and the isolates were identified as follows:( S)-5,6,6 a,7-tetrahydro-2,10-dimethoxy-4 H-dibenzo [DE,G] quinoline-1,9-diol( 1),( +)-isoboldine ß-N-oxide( 2),liriotulipiferine( 3),telitoxinone( 4),isoboldine( 5),(-)-clovane-2ß,9α-diol( 6),benzoic acid( 7),acantrifoside E( 8),and dibutyl phthalate( 9). Among them,compound 1 was new,and compounds 2-9 were reported from this species for the first time.


Assuntos
Medicamentos de Ervas Chinesas/química , Houttuynia/química , Compostos Fitoquímicos/análise , Extratos Vegetais/química , Clorofórmio
11.
Food Chem ; 227: 444-452, 2017 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-28274455

RESUMO

The targeted mass information of compounds accelerated their discovery in a large volume of untargeted MS data. An MS/MS similarity networking is advanced in clustering the structural analogues, which benefits the collection of mass information of similar compounds. The triterpene saponins extracted from Eleutherococcus senticosus leaves (ESL), a kind of functional tea, have shown promise in the relief of Alzheimer's disease. In this work, a target-precursor list (TPL) generated using MS/MS similarity networking was employed to rapidly trace 106 triterpene saponins from the aqueous extracts of ESL, of which 49 were tentatively identified as potentially new triterpene saponins. Moreover, a compound database of triterpene saponins was established and successfully applied to uncover their distribution features in ESL samples collected from different areas.


Assuntos
Eleutherococcus/química , Extratos Vegetais/química , Saponinas/química , Espectrometria de Massas em Tandem/métodos , Triterpenos/química , Folhas de Planta/química
12.
J Pharm Biomed Anal ; 129: 581-592, 2016 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-27521818

RESUMO

Monoterpenoids with "cage-like" pinane skeleton are the unique and main bioactive constituents in peony root, the root of Paeonia lactiflora. A liquid chromatography coupled with ion trap and time-of-flight mass spectrometry (LC-IT-TOF-MS) method was developed for characterization and quantification of monoterpenoids in different types of peony root and the roots of related Paeonia species. MS/MS fragmentation patterns of monoterpenoids with paeoniflorin-, albiflorin- and sulfonated paeoniflorin-type of skeletons were elucidated, which provided basic clues enabling subsequent identification of 35 monoterpenoids in LC-MS profiles of Paeonia species. The profiling analysis and further quantification of 15 main monoterpenoids in 56 samples belonged to red peony root (RPR), white peony root (WPR), peony root in Japanese market (PR) and the roots of related Paeonia species revealed that paeoniflorin, benzoylpaeoniflorin, galloylpaeoniflorin, oxypaoniflorin and albiflorin were predominant constituents in all the samples; mudanpioside C was the characteristic component of P. lactiflora, and 4-O-methyl-paeoniflorin was only detected in P. veitchii and P. anomala. Total contents of the 15 monoterpenoids were obviously higher in the roots of P. lactiflora and P. veitchii than in those of P. anomala and P. japonica. Principal component analysis based on the quantitative results showed that the samples derived from P. lactiflora were clearly classified into RPR, WPR/PR, and sulfur-fumigated WPR groups, besides the respective group of P. veitchii and P. anomala. This study clarified the chemical characteristics of the respective type of peony root and the related Paeonia species, as well as the marker constituents for their discrimination.


Assuntos
Monoterpenos/química , Paeonia/química , Raízes de Plantas/química , Compostos Bicíclicos Heterocíclicos com Pontes/química , Hidrocarbonetos Aromáticos com Pontes/química , Cromatografia Líquida/métodos , Medicamentos de Ervas Chinesas/química , Glucosídeos/química , Espectrometria de Massas em Tandem/métodos
13.
J Nat Prod ; 79(7): 1834-41, 2016 07 22.
Artigo em Inglês | MEDLINE | ID: mdl-27400231

RESUMO

An aqueous extract of Eleutherococcus senticosus leaves exerted a beneficial effect in restoring the neurite outgrowth from Aß25-35-induced degeneration using an axonal density assay. Subsequent bioassay-guided fractionation afforded seven new oleanane-type triterpene saponins, ezoukoginosides A-G (1-7), along with nine known analogues. The structures of 1-7 were elucidated through chemical and spectroscopic approaches, and their effects on restoring the neurite outgrowth from Aß25-35-induced degeneration were investigated. The results revealed that hydrophilic oleanane-type saponins substituted with a free carboxylic acid, hydroxy, or formyl group in the aglycone, especially when the oxidation occurred at C-29, not only restrained Aß25-35-induced degeneration but also restored axonal outgrowth significantly. Compounds 2 (-COOH at C-29) and 3 (-CH2OH at C-29) showed the most potent bioactivity among the isolates.


Assuntos
Eleutherococcus/química , Crescimento Neuronal/efeitos dos fármacos , Ácido Oleanólico/isolamento & purificação , Ácido Oleanólico/farmacologia , Folhas de Planta/química , Saponinas/isolamento & purificação , Saponinas/farmacologia , Peptídeos beta-Amiloides/química , Peptídeos beta-Amiloides/farmacologia , Animais , Feminino , Japão , Camundongos , Estrutura Molecular , Ácido Oleanólico/análogos & derivados , Ácido Oleanólico/química , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/farmacologia , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Gravidez , Saponinas/química
14.
Anal Bioanal Chem ; 408(13): 3555-70, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-26931121

RESUMO

Proanthocyanidins (PAs) are major anti-diarrhea constituents in rhubarb, one of the frequently used traditional medicines. However, the phytochemical investigation of PAs in rhubarb was hampered by their strenuous purification and identification. In the present study, aiming to clarify the distribution of PAs in different rhubarb species, a molecular ion index was priorly established according to the structural features of B-type PAs, which led to a series of targeted discovery of oligomeric PAs in rhubarb by the HPLC-ESI-MS/MS method. Totally, 66 oligomeric PAs including 27 dimers, 29 trimers, and 10 tetramers were tentatively identified on the basis of their MS/MS spectra from 28 rhubarb samples derived from 5 Rheum species as R. palmatum, R. tanguticum, R. officinale, R. coreanum, and R. laciniatum. It is noteworthy that 6 propelargonidins, 14 prodelphinidins, and 10 procyanidin-tetramers were identified from rhubarb for the first time. The profiling comparison of these oligomeric PAs in different rhubarb samples was achieved by visualizing their abundance in a heat map, which indicated the dominant PAs in rhubarb were procyanidin-dimer and its galloylated derivatives.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Proantocianidinas/análise , Rheum/química , Espectrometria de Massas em Tandem/métodos , Especificidade da Espécie , Espectrometria de Massas por Ionização por Electrospray/métodos
15.
Fitoterapia ; 108: 55-61, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26598138

RESUMO

The methanolic extract and its subfractions from red peony root, the dried roots of Paeonia lactiflora Pallas showed potent antiallergic effects, as inhibition of immunoglobulin E (IgE)-mediated degranulation in rat basophil leukemia (RBL)-2H3 cells. Bioassay-guided fractionation led to the isolation of 16 monoterpene derivatives, including 3 new compounds, paeoniflorol (1), 4'-hydroxypaeoniflorigenone (2) and 4-epi-albiflorin (3), together with 13 known ones (4-16). The chemical structures of the new compounds were elucidated on the basis of spectroscopic and chemical evidences. Among the isolated monoterpene derivatives, nine compounds showed potent anti-allergic effects and compound 1 was the most effective. A primary structure-activity relationship of monoterpene derivatives was discussed.


Assuntos
Antialérgicos/farmacologia , Monoterpenos/farmacologia , Paeonia/química , Raízes de Plantas/química , Animais , Antialérgicos/isolamento & purificação , Linhagem Celular Tumoral , Estrutura Molecular , Monoterpenos/isolamento & purificação , Extratos Vegetais/química , Ratos
16.
J Nat Med ; 69(3): 366-74, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25894073

RESUMO

The root of Gentiana crassicaulis has been widely used for the treatment of rheumatism, arthralgia, apoplexy, jaundice and diabetes in traditional medicines, and contains secoiridoid glycosides as the main active ingredients. In the present study, five new secoiridoid glycosides, 6'-O-ß-D-xylopyranosylgentiopicroside (1) and gentiananosides A-D (2-5), together with 11 known ones were isolated from the MeOH extract of dried roots of G. crassicaulis. Their structures were elucidated on the basis of extensive spectroscopic evidence. Of them, gentiananosides A (2) and B (3) were concluded to be novel secoiridoid glycosides with an ether linkage between C-2' of the sugar moiety and C-3 of the aglycone. Compounds 1, 4, 5, 7, 8, 12-14 exhibited moderate inhibitory effects against lipopolysaccharide-induced nitric oxide and interleukin-6 (IL-6) production in RAW264 cells, whereas 2 and 15 exhibited moderate inhibitory effects only against IL-6 production.


Assuntos
Anti-Inflamatórios/farmacologia , Gentiana/química , Glicosídeos Iridoides/farmacologia , Macrófagos/metabolismo , Extratos Vegetais/farmacologia , Animais , Linhagem Celular , Sobrevivência Celular , Avaliação Pré-Clínica de Medicamentos , Interleucina-6/biossíntese , Interleucina-6/farmacologia , Lipopolissacarídeos/farmacologia , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Camundongos , Óxido Nítrico/biossíntese , Raízes de Plantas/química
17.
J Nat Med ; 69(3): 303-12, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25750086

RESUMO

Gentianae Scabrae Radix is a well-known traditional medicine that is used for the treatment of hepatitis, cholecystitis and inflammatory diseases. It consists mainly of secoiridoid glycosides, with representatives of gentiopicroside, sweroside and swertiamarin. In the present study, a chemical investigation of the CHCl3 extract of Gentianae Scabrae Radix derived from Gentiana scabra Bunge yielded seven new (1-7) and ten known (8-17) secoiridoid glycosides. Their structures were elucidated by extensive spectroscopic analyses and comparison with literature data. All 17 compounds were evaluated for their inhibitory effects against NO, IL-6 and TNF-α productions induced by lipopolysaccharide (LPS) in RAW264 cells. Among them, 8-epi-kingiside derivatives 1-3; kingiside derivatives 4, 5 and 10; and a sweroside derivative 6 showed inhibition activity against IL-6 production with IC50 values of 51.70-61.10 µM, whereas sweroside derivatives 12 and 15-17 and a swertiamarin derivative 13 showed inhibition effects on both NO and IL-6 productions with IC50 values of 64.74-94.95 and 48.91-75.45 µM, respectively. All the compounds exhibited weak inhibitory activity (IC50 > 100 µM) in a TNF-α bioassay. Finally, a primary structure-activity relationship of these secoiridoid glycosides is discussed.


Assuntos
Anti-Inflamatórios/farmacologia , Gentiana/química , Glicosídeos Iridoides/farmacologia , Rizoma/química , Animais , Linhagem Celular , Avaliação Pré-Clínica de Medicamentos , Interleucina-6/metabolismo , Lipopolissacarídeos/farmacologia , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Camundongos , Estrutura Molecular , Fator de Necrose Tumoral alfa/metabolismo
18.
Phytochemistry ; 86: 201-7, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23098900

RESUMO

Two aristololactams, aristololactam GI (1) and aristololactam GII (2), and three aporphines, fissistigamide A (3), fissistigamide B (4) and fissistigmine (5), together with nineteen known alkaloids, one flavone and one anthraquinone were isolated from the ethanol extracts of the stems of Fissistigma oldhamii (Annonaceae). Their structures were elucidated primarily by analysis of NMR, IR, UV, MS and CD data. Alkaloid 1 is a chiral aristololactam formed from a phenylpropanoid derivative attached to a 3,4-dihydroxy aristololactam scaffold. The absolute configuration of 1 was determined by comparing experimental and calculated ECD spectra. The anti-inflammatory activity of the crude extracts and the five alkaloids were tested by measuring the amount of TNF-α and IL-6 released from LPS stimulated RAW264 cell via ELISA. The results demonstrated that the CHCl(3)-soluble part and alkaloid 2 exhibited significant anti-inflammatory activity in vitro in both assays.


Assuntos
Annonaceae/química , Anti-Inflamatórios/química , Aporfinas/química , Aporfinas/farmacologia , Animais , Anti-Inflamatórios/farmacologia , Linhagem Celular , Interleucina-6/metabolismo , Espectroscopia de Ressonância Magnética , Camundongos , Estrutura Molecular , Fator de Necrose Tumoral alfa/metabolismo
19.
Fitoterapia ; 81(5): 367-70, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-19909799

RESUMO

Constituents of the fruit of Sinopodophyllum hexandrum (Royle) Ying (Sinopodophylli Fructus) were investigated. A new flavonoid, 8,2'-diprenylquercetin 3-methyl ether along with 9 known compounds were isolated and identified. Among them, the new compound 8,2'-diprenylquercetin 3-methyl ether exhibited cytotoxic activities against MDA-231 and T47D breast cancer cell lines, quercetin, kaempferol and rutin were isolated from Sinopodophylli Fructus for the first time.


Assuntos
Antineoplásicos Fitogênicos/uso terapêutico , Berberidaceae/química , Neoplasias da Mama/tratamento farmacológico , Fitoterapia , Extratos Vegetais/uso terapêutico , Quercetina/análogos & derivados , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Linhagem Celular Tumoral , Feminino , Flavonoides/isolamento & purificação , Flavonoides/farmacologia , Flavonoides/uso terapêutico , Frutas , Humanos , Quempferóis/isolamento & purificação , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia , Quercetina/isolamento & purificação , Quercetina/farmacologia , Quercetina/uso terapêutico , Rutina/isolamento & purificação
20.
Zhongguo Zhong Yao Za Zhi ; 32(23): 2461-7, 2007 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-18330232

RESUMO

The recent progress in the studies on chemical constituents, pharmacological activity and quality control of the medical plants of genus Curcuma were summarized. These plants contain various chemical constituents and have broad bioactivities such as anti-oxidation activity, anti-tumor and anti-inflammatory. It is complex when it is used for traditional Chinese medicine(TCM) because of its multispeciese and multiresources. The author believe that a deep research of the quality criterion about this genus can solve many problems of the further investigation and ensure the safety and utility of TCM.


Assuntos
Curcuma/química , Curcumina/isolamento & purificação , Plantas Medicinais/química , Sesquiterpenos/isolamento & purificação , Animais , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Curcuma/classificação , Curcumina/química , Curcumina/farmacologia , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Humanos , Estrutura Molecular , Sesquiterpenos/química , Sesquiterpenos/farmacologia
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