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1.
Kaohsiung J Med Sci ; 2020 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-32841520

RESUMO

Accumulating evidences have suggested that exosomes are closely associated with tumor progression by affecting cell-cell communication. Here, we aimed to investigate the roles and regulatory mechanism of exosomes released from chronic lymphocytic leukemia (CLL). The expression levels of genes and proteins in cells and exosomes were examined by quantitative real-time PCR and Western blotting, respectively. MEC-1 cell-derived exosomes were obtained and co-cultured with human umbilical vein endothelial cells (HUVECs), then the capabilities of cell proliferation, metastasis and angiogenesis of HUVECs were measured by CCK-8, wound healing, transwell and tube formation assay, respectively. Chloride intracellular channel 1 (CLIC1) was significantly increased in CLL patients and markedly enriched in exosomes secreted by CLL cells. Exosomal CLIC1 secreted from MEC-1 cells were successfully transferred into HUVECs and significantly promoted the phenotypes of proliferation, metastasis and angiogenesis of HUVECs. Mechanically, exosomal CLIC1 secreted from MEC-1 cells obviously activated MAPK/ERK signaling through upregulating integrin ß1 (ITGß1) expression in HUVECs. Furthermore, rescue experiments revealed that either silencing ITGß1 or PD98059 treatment obviously reversed the regulatory effects of exosomal CLIC1 secreted from MEC-1 cells in HUVECs. In conclusion, CLL cell-derived exosomes accelerated HUVECs metastasis and angiogenesis through transferring CLIC1 to regulate ITGß1-MAPK/ERK signaling, indicating that CLIC1 may be a therapeutic target of CLL exosomes in the tumor microenvironment.

2.
Sheng Li Xue Bao ; 72(3): 308-324, 2020 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-32572429

RESUMO

Gut injury continues to be the devastating and unpredictable critical illness associated with increased cell death of intestinal epithelial cells (IECs). The IECs, immune system and microbiome are the interrelated entities to maintain normal intestinal homeostasis and barrier integrity. In response to microbial invasion, IEC cell death occurs to maintain intestinal epithelium function and retain the continuous renewal and tissue homeostasis. But the imbalance of IEC cell death results in increased intestinal permeability and barrier dysfunction that leads to several acute and chronic intestinal diseases, such as intestinal ischemia/reperfusion (I/R), sepsis, inflammatory bowel diseases (IBD), necrotizing enterocolitis (NEC), etc. During the pathophysiological state, the excessive IEC apoptotic cell death leads to a chronic inflammatory condition, later switches to necroptotic cell death mechanism that induces more pathological features than apoptosis and may also induce other lytic cell death mechanisms like pyroptosis and ferroptosis to increase the pathogenesis of the intestinal diseases. But still, there remains gaps in the fundamental knowledge about the IEC cell death mechanisms in chronic intestinal diseases. Together, a deep understanding of the specific cell death mechanisms underlying chronic intestinal diseases, including sepsis, IBD, NEC, and intestinal I/R, is desperately needed to develop emerging novel promising therapeutic strategies. This review aims to show how the acute and critical illness in the gut are driven by IEC cell death mechanism, such as apoptosis, necrosis, necroptosis, pyroptosis, and ferroptosis.


Assuntos
Células Epiteliais , Mucosa Intestinal , Apoptose , Morte Celular , Humanos , Recém-Nascido , Necrose
3.
Artigo em Inglês | MEDLINE | ID: mdl-32431143

RESUMO

Creating nano-to-macroscopic-sized artificial actuators in response to light has been a challenging issue. Herein, we describe the design, synthesis, and operation of a photomechanical molecular crystal (PMMC) that exhibits well-controlled multiple photo-driven motions, including translation, rotation, and jumping, by adjusting the irradiation sites. Theoretical calculation discloses that conversion of light energy into macroscopic motion occurs through a molecular conformation change between the excited and ground states mediated by ultrafast conical internal conversion, making the photomechanical/recovery responses a rapid cycle. Therefore, our PMMCs can complete the directional and continuous motions using only one laser beam. We also demonstrated the actuated rotation of a cross-shaped sample by rotating the polarization of the laser beam at a rate of >2 Hz, like a dancer under a spotlight. This finding could lead to remote-controlled micrometer-sized vehicles and valves on solid substrates.

4.
Chemphyschem ; 21(9): 952-957, 2020 05 05.
Artigo em Inglês | MEDLINE | ID: mdl-32182404

RESUMO

A recent experiment [Angew. Chem. Int. Ed. 2017, 56, 722-727] found that a (1 : 9) blend film of two anthracene derivatives, 2-fluorenyl-2-anthracene (FlAnt) and 2-anthryl-2-anthracence (2 A), exhibit both efficient white light emission and high hole mobility, thus promising for organic light-emitting transistors (OLETs). Employing quantum chemistry at the polarizable continuum model (PCM) and the quantum mechanics/molecular mechanics (QM/MM) levels, we investigated the excited-state structures, optical spectra, band structure and the carrier mobility for FlAnt and 2 A from solution to aggregate phases. We suggest using the ratio of intermolecular excitonic coupling J and intramolecular excited state relaxation energy E to judge the bathochromic shift in optical emission in aggregates. For FlAnt, ρ=J/E is calculated to be less than 0.17, a critical value we identified earlier, and the spectra in solution and aggregate phases present quite similar features (blue emission). However, ρ is ∼0.5 for 2 A systems, and the calculated emission in the aggregate phase exhibits a remarkable bathochromic shift. In addition, the 0-0 emission is strongly suppressed in the herringbone stacking. These observations justify the experimental findings that (i) 2 A is blue emissive in solution but yellow-green in the aggregate phase, whereas FlAnt is always blue, and (ii) the blend of them show white emission. By using the "quantum nuclear tunneling" model we proposed earlier, we found the hole mobility for FlAnt and 2 A are 0.5 and 4.2 cm2 V-1 s-1 , respectively, indicating both are good hole transport materials.

5.
Artigo em Inglês | MEDLINE | ID: mdl-32049548

RESUMO

Sirtuin 6 (Sirt6) is predominantly expressed in epithelial cells in intestinal crypts. It plays an important role in protecting intestinal epithelial cells against inflammatory injury. Previously, we found that colitis is associated with the downregulation of Sirt6 protein in the intestines. Here, we report that murine interferon-γ (Ifnγ) inhibits Sirt6 protein but not mRNA expression in young adult mouse colonocytes (YAMC, a mouse colonic epithelial cell line) in a dose- and time-dependent manner. Using microRNA array analysis, we showed that Ifnγ induces expression of miR-92b in YAMC cells. With in silico analysis, we found that the Sirt6 3'-untranslated region (UTR) contains a putative binding site for miR-92b. Luciferase assay showed that Ifnγ inhibited Sirt6 3'-UTR activity and this effect was mimicked by miR-92b via directly targeting the miR-92b seed site in the 3'-UTR of Sirt6 mRNA. Furthermore, Western blot demonstrated that miR-92b downregulated Sirt6 protein expression in YAMC cells. Blocking miR-92b with a specific inhibitor attenuated the inhibitory effect of Ifnγ on Sirt6 protein expression in the cells. Collectively, our data suggest that Ifnγ inhibits Sirt6 protein expression in intestinal epithelial cells via a miR-92b-mediated mechanism. miR-92b may be a novel therapeutic target for rescuing Sirt6 protein levels in intestinal epithelial cells, thereby protecting against intestinal mucosal injury caused by inflammation.


Assuntos
Células Epiteliais/metabolismo , Interferon gama/metabolismo , MicroRNAs/genética , Sirtuínas/genética , Animais , Linhagem Celular , Células Epiteliais/efeitos dos fármacos , Expressão Gênica/efeitos dos fármacos , Inflamação/tratamento farmacológico , Inflamação/genética , Inflamação/metabolismo , Interferon gama/farmacologia , Mucosa Intestinal/metabolismo , Intestinos/efeitos dos fármacos , Sirtuínas/metabolismo
6.
Cancer Biomark ; 27(4): 525-532, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32083571

RESUMO

BACKGROUND: Lung adenocarcinoma is the most common type of lung cancer, and it is one of the most aggressive and rapidly fatal tumor types. OBJECTIVE: To identify a signature mutation genes for prognostic prediction of lung adenocarcinoma. METHODS: Four hundred and sixty-two lung adenocarcinoma cases were screened out and downloaded from TCGA database. Mutation data of 18 targeted genes were detected by MuTect. LASSO-COX model was used to screen gene loci, and then a prognosis model was established. Afterwards, 40 clinical patients of lung adenocarcinoma were collected to verify the mutation features and the predictive function of the above prognostic model. The mutations of above 18 genes were sequenced with targeted next generation sequencing (NGS) and analyzed with GATK and MuTect. RESULTS: TP53 (282, 32.38%), NF1 (82, 9.41%) and EGFR (80, 9.18%) were the top 3 most frequent mutation genes. A total of 7 variables were screened out after lasso-COX analysis (tumor stage, age, diagnostic type, SMARCA4, GNAS, PTCH2, TSC2). SMARCA4, GNAS and TSC2 were a gene mutation signature to predict a poor prognosis. CONCLUSIONS: We established a prognostic model for lung adenocarcinoma, and further concluded that SMARCA4, GNAS and TSC2 were a gene signature which plays a prognostic role.

7.
J Chem Phys ; 152(2): 024118, 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31941306

RESUMO

It is believed that the density functional theory (DFT) describes most elements with s, p, and d orbitals very well, except some materials that have strongly localized and correlated valence electrons. In this work, we find that the widely employed exchange-correlation (XC) functionals, including local-density approximation (LDA), generalized gradient approximation (GGA), and meta-GGA, underestimate the shear modulus and phase stability of V and Nb greatly. The advanced hybrid functional that is usually better for correlated systems, on the other hand, completely fails in these two simple metals. This striking failure is revealed due to the orbital localization error in GGA, which is further deteriorated by hybrid functionals. This observation is corroborated by a similar failure of DFT+U and van der Waals functionals when applied to V and Nb. To remedy this problem, a semiempirical approach of DFT+J is proposed, which can delocalize electrons by facilitating the on-site exchange. Furthermore, it is observed that including density derivatives slightly improves the performance of the semilocal functionals, with meta-GGA outperforms GGA, and the latter is better than LDA. This discovery indicates the possibility and necessity to include higher-order density derivatives beyond the Laplacian level for the purpose of removing the orbital localization error (mainly from d orbitals) and delocalization error (mainly from s and p orbitals) completely in V and Nb so that a better description of their electronic structures is achieved. The same strategy can be applied to the other d electron system and f electron system.

8.
Nat Biomed Eng ; 4(3): 343-354, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31768001

RESUMO

Imaging techniques for quantifying changes in the hierarchical structure of deforming joints are constrained by destructive sample treatments, sample-size restrictions and lengthy scan times. Here, we report the use of fast low-dose pink-beam synchrotron X-ray tomography in combination with mechanical loading at nanometric precision for in situ imaging, at resolutions below 100 nm, of the mechanical strain in intact untreated joints under physiologically realistic conditions. We show that in young, older and osteoarthritic mice, hierarchical changes in tissue structure and mechanical behaviour can be simultaneously visualized, and that the tissue structure at the cellular level correlates with the mechanical performance of the whole joint. We also use the tomographic approach to study the colocalization of tissue strains to specific chondrocyte lacunar organizations within intact loaded joints and to explore the role of calcified-cartilage stiffness on the biomechanics of healthy and pathological joints.


Assuntos
Articulações/diagnóstico por imagem , Síncrotrons , Tomografia por Raios X/métodos , Animais , Condrócitos/ultraestrutura , Imageamento Tridimensional , Articulações/ultraestrutura , Masculino , Camundongos , Nanoestruturas , Osteoartrite/diagnóstico por imagem , Osteoartrite/patologia , Estresse Mecânico
9.
Chem Commun (Camb) ; 56(6): 912-915, 2020 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-31850456

RESUMO

A novel spiro-fused terrylene dimer (SDT) was designed and synthesized by a dissymmetric spiro-fusion strategy. The spiro-conjugation effect caused a distinct red-shift and enhancement of the absorption spectrum. Two chiral enantiomers of SDT have been absolutely resolved and identified in combination with theoretical calculations. Circularly polarized luminescence (CPL) measurement revealed its potential as a near-infrared chiral luminescent material.

10.
Exp Ther Med ; 18(6): 4820-4828, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31772647

RESUMO

Dexmedetomidine (DEX) is widely used in perioperative settings for analgesia and sedation; however, little is known about its effects on the hypoxia-induced progression of tumor cells. In the present study, the effects of DEX on hypoxia-induced growth and metastasis of lung cancer cells and colorectal cancer cells was examined. A549 cells and HCT116 cells were treated with normoxia, hypoxia, co-treatment of hypoxia and DEX, and atipamezole (an α2 adrenoceptor antagonist) for 4 h. The proliferation rate of cells was determined by MTT assays. Cell metastatic potential was evaluated by Transwell assays. Survivin and hypoxia inducible factor (HIF)-1α were detected by western blotting. Matrix metalloproteinase (MMP)-2 and MMP-9 were measured using reverse transcription-quantitative PCR. It was demonstrated that hypoxia treatment promoted the proliferation and may promote the metastasis of the two cancer cell lines. DEX substantially contributed to the survival and aggressiveness of the two cancer cell lines following hypoxia. Furthermore, DEX upregulated the expression of survivin, MMP-2, MMP-9 and HIF-1α in the two cancer cell lines in response to hypoxia. Finally, the effects of DEX on the hypoxia-induced growth and metastatic potential of cancer cells were reversed by atipamezole. Collectively, DEX enhances the hypoxia-induced progression of lung cancer cells and colorectal cancer cells by regulating HIF-1α signaling, which may be associated with the α2 adrenoceptor pathway.

11.
J Exp Clin Cancer Res ; 38(1): 386, 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31481087

RESUMO

BACKGROUND: Renal cell carcinoma (RCC) is a deadly urological tumor that remains largely incurable. Our limited understanding of key molecular mechanisms underlying RCC invasion and metastasis has hampered efforts to identify molecular drivers with therapeutic potential. With evidence from our previous study revealing that nuclear overexpression of YBX1 is associated with RCC T stage and metastasis, we investigated the effects of YBX1 in RCC migration, invasion, and adhesion, and then characterized its interaction with RCC-associated proteins G3BP1 and SPP1. METHODS: Renal cancer cell lines, human embryonic kidney cells, and clinical samples were analyzed to investigate the functional role of YBX1 in RCC metastasis. YBX1 knockdown cells were established via lentiviral infection and subjected to adhesion, transwell migration, and invasion assay. Microarray, immunoprecipitation, dual-luciferase reporter assay, and classical biochemical assays were applied to characterize the mechanism of YBX1 interaction with RCC-associated proteins G3BP1 and SPP1. RESULTS: Knockdown of YBX1 in RCC cells dramatically inhibited cell adhesion, migration, and invasion. Mechanistic investigations revealed that YBX1 interaction with G3BP1 upregulated their downstream target SPP1 in vitro and in vivo, which led to an activated NF-κB signaling pathway. Meanwhile, knockdown of SPP1 rescued the YBX1/G3BP1-mediated activation of NF-κB signaling pathway, and RCC cell migration and invasion. We further showed that YBX1 expression was positively correlated with G3BP1 and SPP1 expression levels in clinical RCC samples. CONCLUSIONS: YBX1 interacts with G3BP1 to promote metastasis of RCC by activating the YBX1/G3BP1-SPP1-NF-κB signaling axis.


Assuntos
Carcinoma de Células Renais/metabolismo , Carcinoma de Células Renais/patologia , DNA Helicases/metabolismo , Neoplasias Renais/metabolismo , Neoplasias Renais/patologia , NF-kappa B/metabolismo , Osteopontina/metabolismo , Proteínas de Ligação a Poli-ADP-Ribose/metabolismo , RNA Helicases/metabolismo , Proteínas com Motivo de Reconhecimento de RNA/metabolismo , Proteína 1 de Ligação a Y-Box/metabolismo , Animais , Carcinoma de Células Renais/genética , Adesão Celular , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Genes Reporter , Humanos , Neoplasias Renais/genética , Camundongos , Osteopontina/genética , Interferência de RNA , RNA Interferente Pequeno/genética , Transdução de Sinais , Ensaios Antitumorais Modelo de Xenoenxerto
12.
ACS Omega ; 4(9): 13640-13644, 2019 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-31497681

RESUMO

Pressure significantly modifies the microscopic interactions in the condense phase, leading to new patterns of bonding and unconventional chemistry. Using unbiased structure searching techniques combined with first-principles calculations, we demonstrate the reaction of argon with xenon at a pressure as low as 1.1 GPa, producing a novel van der Waals compound XeAr2. This compound is a wide-gap insulator and crystallizes in a MgCu2-type Laves phase structure. The calculations of phonon spectra and formation enthalpy indicate that XeAr2 would be stable without any phase transition or decomposition at least up to 500 GPa.

13.
Phys Chem Chem Phys ; 21(27): 15167-15176, 2019 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-31246206

RESUMO

Weyl semimetal, a newly developed thermoelectric material, has aroused much interest due to its extraordinary transport properties. In this work, the thermoelectric transport properties of NbX (X = P and As), a prototypical Weyl semimetal, are investigated using the first-principles calculations together with Boltzmann transport theory. The calculated room-temperature lattice thermal conductivities along the a and c directions are 2.0 W mK-1 and 0.6 W mK-1 for NbP and 1.4 W mK-1 and 0.4 W mK-1 for NbAs, respectively. The low thermal conductivities may be useful in the thermoelectric applications. It is found that the acoustic branches have obvious contribution to the total lattice thermal conductivity, and the size dependence of the thermal conductivities can provide guidance for designing thermoelectric nanostructures. Our results show that the anisotropic structures of these compounds bring about the anisotropy of transport coefficients along the a and c directions, and the preferred direction is the c direction in thermoelectric applications. Moreover, NbP and NbAs show high ZT values of 0.82 and 0.50 along the c direction for p-type at an optimal carrier concentration, indicating that they are potential thermoelectric materials.

14.
ACS Appl Mater Interfaces ; 11(17): 15837-15844, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-30964258

RESUMO

n-Type organic/polymeric semiconductors with high electron mobilities are highly demanded for future flexible organic circuits. Except for developing a new conjugated backbone, recent studies show that side-chain engineering also plays an indispensable role in boosting the charge-transporting property. In this paper, we report a new polymer PNDI2T-DTD with a representative n-type naphthalene diimide (NDI)-bithiophene backbone for high-performance n-type flexible thin-film transistors through branching/linear side-chain engineering strategy. Serving as the flexible side chains, the linear/branching side-chain pattern is found to be effective in tuning the preaggregation behavior in solution and the packing ordering of polymeric chains, resulting in the improvement of thin-film crystallinity. The electron mobility of the thin film of PNDI2T-DTD on flexible substrates can reach 1.52 cm2 V-1 s-1, which is approximately five times higher than that of PNDI2T-DT with the same conjugated backbone and only branching alkyl chains.

15.
Cancer Sci ; 110(5): 1609-1620, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30868675

RESUMO

Cancer tissues consist of cancer cells, surrounding stromal cells and the extracellular matrix. Cancer-associated fibroblasts (CAF) are one of the key components of stromal cells. CAF have a great impact on the behavior of cancer cells, including proliferation, invasion, metastasis and chemoresistance in many ways. However, the underlying mechanism had not been fully elucidated. In this study, we investigated the role of CAF in cisplatin resistance of lung cancer cells. By using conditioned medium from CAF (CAF-CM), we found that CAF decreased the sensitivity of lung cancer cells to cisplatin. RNA sequencing results showed that CAF expressed a higher level of Annexin A3 (ANXA3) than normal fibroblasts (NF), and CAF-CM incubation increased the ANXA3 level in lung cancer cells. Overexpression of ANXA3 in lung cancer cells increased cisplatin resistance and activated c-jun N-terminal kinase (JNK), whereas knockdown of ANXA3 increased cisplatin sensitivity. Further study showed that CAF-CM enhanced cisplatin resistance by inhibiting cisplatin-induced apoptosis, determined by repression of caspase-3 and caspase-8, through activation of the ANXA3/JNK pathway. Conversely, suppression of JNK activation by specific inhibitor retarded the effect of CAF-CM and ANXA3 on cisplatin sensitivity. Taken together, our study demonstrated that CAF potentiated chemoresistance of lung cancer cells through a novel ANXA3/JNK pathway both in vitro and in vivo, suggesting ANXA3 could be a potential therapeutic target for the treatment of chemoresistant cancer.


Assuntos
Anexina A3/genética , Fibroblastos Associados a Câncer/citologia , Cisplatino/farmacologia , Resistencia a Medicamentos Antineoplásicos , Neoplasias Pulmonares/patologia , Células A549 , Animais , Anexina A3/metabolismo , Apoptose/efeitos dos fármacos , Fibroblastos Associados a Câncer/metabolismo , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Meios de Cultivo Condicionados/farmacologia , Humanos , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/metabolismo , Camundongos , Análise de Sequência de RNA/métodos , Células Tumorais Cultivadas , Regulação para Cima , Ensaios Antitumorais Modelo de Xenoenxerto
16.
J Chem Theory Comput ; 15(3): 1477-1491, 2019 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-30620581

RESUMO

Despite great attention to the charge transport in organic semiconductors (OSC) over the last decades, the underlying mechanism is still controversial. After our theoretical position in 2009, the quantum nuclear tunneling effect has been proven by more and more experiments to play an essential role for charge transport in organic and polymeric materials. On the other hand, back in the 1970s, it was proposed that the nature of charge transport could be analyzed by the isotope effect, which, however, has not been confirmed either experimentally or theoretically. In this Perspective, we review the understanding of microscopic mechanisms on charge transport by using different transport mechanisms from hopping to band transport. Particularly, we point out that the isotope effect, which is absent in the semiclassical Marcus theory, should be negative for the localized charge transport with quantum nuclear tunneling. We conclude that the quantum nuclear tunneling effect dominates the charge transport in OSCs.

17.
Nanoscale Res Lett ; 13(1): 376, 2018 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-30467605

RESUMO

Hydrogenated graphene (HG)/hexagonal boron nitride (h-BN) heterobilayer is an ideal structure for the high-performance field effect transistor. In this paper, the carrier mobilities of HG/h-BN heterobilayer are investigated based on the first-principles calculations by considering the influence of stacking pattern between HG and h-BN, hydrogen coverage and hydrogenation pattern. With the same hydrogenation pattern, the electron mobility monotonously decreases when the hydrogen coverage increases. With the same hydrogen coverage, different hydrogenation patterns lead to significant changes of mobility. For 25% and 6.25% HGs, the µe (ΓK) of 25% pattern I is 8985.85 cm2/(V s) and of 6.25% pattern I is 23,470.98 cm2/(V s), which are much higher than other patterns. Meanwhile, the h-BN substrate affects the hole mobilities significantly, but it has limit influences on the electron mobilities. The hole mobilities of stacking patterns I and II are close to that of HG monolayer, but much lower than that of stacking patterns III and IV.

18.
J Comput Chem ; 39(31): 2582-2588, 2018 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-30431662

RESUMO

Metal organic coordination polymers (MOCPs) provide an intriguing platform to design functional thermoelectric materials through modifying metal atoms, organic ligands, etc. Based on density functional theory (DFT) coupled with Boltzmann transport theory, the thermoelectric properties of several MOCPs, which is designed by intercalating organic linkers ranging from benzene to pentacene between two inorganic units, have been investigated. We found that the interplay of d orbital of Ni atom and π orbitals of the organic linkers play an important role in band engineering and then thermoelectric efficiency. Combining the high conductivity for π orbitals of organic ligands and high Seebeck coefficient of the d orbital of Ni atom, such intercalated MOCPs provide new way to design high performance thermoelectric materials. © 2018 Wiley Periodicals, Inc.

19.
Cell Death Dis ; 9(5): 501, 2018 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-29717134

RESUMO

The chronic inflammatory microenvironment within or surrounding the primary renal cell carcinoma (RCC) site promotes oncogenic transformation as well as contributes to the development of metastasis. G3BP stress granule assembly factor 1 (G3BP1) was found to be involved in the regulation of multiple cellular functions. However, its functions in RCC have not been previously explored. Here, we first showed that the expression of G3BP1 is elevated in human RCC and correlates with RCC progression. In cultured RCC cells, knockdown of G3BP1 results in inhibition of tumor cell proliferation, migration, and invasion, consistently with the alteration of epithelial-mesenchymal transition (EMT) and cell proliferative markers, including Cadherins, Vimentin, Snail, Slug, c-Myc, and cyclin D1. Remarkably, knockdown of G3BP1 dramatically impaired the signaling connection of pro-inflammatory cytokine IL-6 stimulation and downstream STAT3 activation in RCC, thus eventually contributing to the disruption of IL-6-elicited RCC migration and metastasis. In addition, in vivo orthotopic tumor xenografts results confirmed that knockdown of G3BP1 suppressed RCC tumor growth and metastasis in mice. Collectively, our findings support the notion that G3BP1 promotes tumor progression and metastasis through IL-6/G3BP1/STAT3 signaling axis in RCC.


Assuntos
Carcinoma de Células Renais/genética , DNA Helicases/genética , Regulação Neoplásica da Expressão Gênica , Interleucina-6/genética , Neoplasias Renais/genética , Neoplasias Hepáticas/genética , Neoplasias Pulmonares/genética , Proteínas de Ligação a Poli-ADP-Ribose/genética , RNA Helicases/genética , Proteínas com Motivo de Reconhecimento de RNA/genética , Fator de Transcrição STAT3/genética , Idoso , Animais , Caderinas/genética , Caderinas/metabolismo , Carcinoma de Células Renais/metabolismo , Carcinoma de Células Renais/secundário , Carcinoma de Células Renais/cirurgia , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Ciclina D1/genética , Ciclina D1/metabolismo , DNA Helicases/antagonistas & inibidores , DNA Helicases/metabolismo , Feminino , Humanos , Interleucina-6/metabolismo , Neoplasias Renais/metabolismo , Neoplasias Renais/patologia , Neoplasias Renais/cirurgia , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/secundário , Neoplasias Hepáticas/cirurgia , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/secundário , Neoplasias Pulmonares/cirurgia , Masculino , Camundongos , Pessoa de Meia-Idade , Gradação de Tumores , Nefrectomia , Proteínas de Ligação a Poli-ADP-Ribose/antagonistas & inibidores , Proteínas de Ligação a Poli-ADP-Ribose/metabolismo , RNA Helicases/antagonistas & inibidores , RNA Helicases/metabolismo , Proteínas com Motivo de Reconhecimento de RNA/antagonistas & inibidores , Proteínas com Motivo de Reconhecimento de RNA/metabolismo , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo , Fator de Transcrição STAT3/metabolismo , Transdução de Sinais , Fatores de Transcrição da Família Snail/genética , Fatores de Transcrição da Família Snail/metabolismo , Carga Tumoral , Vimentina/genética , Vimentina/metabolismo , Ensaios Antitumorais Modelo de Xenoenxerto
20.
Chem Commun (Camb) ; 54(53): 7294-7297, 2018 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-29717308

RESUMO

A rylene annulated phthalocyanine (ZnPcPDI4) was designed and synthesized. Single-crystal X-ray diffraction analyses unambiguously confirmed the giant molecule quasi-planar "four leaf clover" skeleton with a 27 Å diameter and a two-dimensional cage-like supramolecular structure. The fully conjugated characteristics endowed it with significant Q-band red-shifts to the near-infrared region of 785 nm, and a largely degenerated LUMO level.

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