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1.
Neoplasma ; 2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34818025

RESUMO

Hepatocellular carcinoma (HCC) is a major cause of cancer-related deaths worldwide. Emerging evidence has revealed the vital functions of microRNAs (miRNAs) in cancer malignant progressions. miR-375 has been verified to serve as an antioncogene in tumorigenesis and a potential therapeutic target in various types of cancer. In this study, we aimed to determine the role of miR-375 in the regulation of chemoresistance and metastasis of HCC. Differentially expressed miR-375 and NCAPG2 were externally validated using expression data from The Cancer Genome Atlas (TCGA) database. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the expression levels of miR-375 in HCC tissues and cell lines. miR-375 mimics and NCAPG2-overexpression were transfected into HepG2 and Huh7 cells to establish miR-375 overexpression models. Cell Counting Kit-8, Transwell, and flow cytometry experiments were conducted to monitor cell proliferation, migration, and apoptosis. The targeting relationship between miR-375 and non-SMC condensin II complex subunit G 2 (NCAPG2) was determined by qRT-PCR, western blot, and luciferase reporter gene assay. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were conducted using Gene Set Enrichment Analysis (GSEA). The pathway enrichment analysis was used to predict the potential pathways for further study. miR-375 was significantly downregulated in HCC tissues and cells compared to adjacent tissue and normal hepatocyte cell line respectively while NCAPG2 was upregulated. The targeting relationship was verified by luciferase reporting assay, and miR-375 could target the 3'UTR of NCAPG2 mRNA and effectively suppress NCAPG2 protein expression. Replenishing of miR-375 significantly repressed HCC cell proliferation and migration, and induced cell apoptosis. Overexpression of NCAPG2 recovered those biological abilities in miR-375 overexpressed cells. Collective data suggested that miR-375 served as a tumor suppressor via regulating NCAPG2. Replenishing of miR-375 or knockout of NCAPG2 could be therapeutically exploited for HCC.

2.
Brain Stimul ; 15(1): 35-45, 2021 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-34752934

RESUMO

BACKGROUND: Deficits in associative memory (AM) are the earliest and most prominent feature of Alzheimer's disease (AD) and demonstrate a clear cause of distress for patients and their families. OBJECTIVE: The present study aimed to determine AM enhancements following accelerated intermittent theta-burst stimulation (iTBS) in patients with AD. METHODS: In a randomized, double-blind, sham-controlled design, iTBS was administered to the left dorsolateral prefrontal cortex (DLPFC) of patients with AD for 14 days. Measurements included AM (primary outcome) and a comprehensive neuropsychological battery. Patients were evaluated at baseline, following the intervention (week 2), and 8 weeks after treatment cessation (week 10). RESULTS: Sixty patients with AD were initially enrolled; 47 completed the trial. The active group displayed greater AM improvements compared with the sham group at week 2 (P = 0.003), which was sustained at week 10. Furthermore, higher Mini-Mental State Examination (MMSE) scores at baseline were associated with greater AM improvements at weeks 2 and 10. For the independent iTBS group, this correlation predicted improvements in AM (P < 0.001) and identified treatment responders with 92% accuracy. Most of the neuropsychological tests were markedly improved in the active group. In particular, the Montreal Cognitive Assessment and MMSE in the active group increased by 2.8 and 2.3 points, respectively, at week 2, while there was no marked change in the sham group. CONCLUSION: In the present study, accelerated iTBS of the DLPFC demonstrated an effective and well-tolerated complementary treatment for patients with AD, especially for individuals with relatively high MMSE scores.

3.
Artigo em Inglês | MEDLINE | ID: mdl-34783141

RESUMO

As a revolutionary cancer treatment, the chimeric antigen receptor (CAR) T cell therapy suffers from complications such as cytokine release syndromes and T cell exhaustion. Their mitigation desires controllable activation of CAR-T cells that is achievable through regulatory display of CARs. By embedding the hepatitis C virus NS3 protease (HCV-NS3) between the single-chain variable fragment (scFv) and the hinge domain, we showed that the display of anti-CD19 scFv on CAR-T cells was positively correlated to the presence of a clinical HCV-NS3 inhibitor asunaprevir (ASV). This novel CAR design that allows the display of anti-CD19 scFv in the presence of ASV and its removal in the absence of ASV creates a practically recurring chemical switch. We demonstrated that the intact CAR on T cells can be repeatedly turned on and off by controlling the presence of ASV in a dose dependent manner both in vitro and in vivo, which enables delicate modulation of CAR-T activation during cancer treatment.

4.
Stat Med ; 2021 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-34729799

RESUMO

With the advent of the big data era, the need to combine multiple individual data sets to draw causal effects arises naturally in many medical and biological applications. Especially each data set cannot measure enough confounders to infer the causal effect of an exposure on an outcome. In this article, we extend the method proposed by a previous study to causal data fusion of more than two data sets without external validation and to a more general (continuous or discrete) exposure and outcome. Theoretically, we obtain the condition for identifiability of exposure effects using multiple individual data sources for the continuous or discrete exposure and outcome. The simulation results show that our proposed causal data fusion method has unbiased causal effect estimate and higher precision than traditional regression, meta-analysis and statistical matching methods. We further apply our method to study the causal effect of BMI on glucose level in individuals with diabetes by combining two data sets. Our method is essential for causal data fusion and provides important insights into the ongoing discourse on the empirical analysis of merging multiple individual data sources.

5.
J Colloid Interface Sci ; 608(Pt 1): 459-469, 2021 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-34626989

RESUMO

Carbon nanotubes (CNTs) are often used to settle down the sluggish reaction kinetics in lithium-sulfur batteries (LSBs). However, the self-aggregation of CNTs often makes them fail to effectively inhibit the shuttling effect of soluble lithium polysulfide (LiPS) intermediates. Herein, a type of ultra-stable carbon micro/nano-scale interconnected "carbon cages" has been designed by incorporating polar acid-treated carbon fibers (ACF) into three-dimensional (3D) CNT frameworks during vacuum filtration processes. Results show that the ACF-CNT composite frameworks possess a reinforced-concrete-like structure, in which the ACFs can well work as the main mechanical supporting frames for the composite electrodes, and the oxygen-containing functional groups (OFGs) formed on them as cross linker between ACFs and CNTs. Benefitted from this design, the ACF-CNT/S cathodes deliver an excellent rate capability (retain 72.6% at 4C). More impressively, the ACF-CNT/S cathodes also show an ultrahigh cycling stability (capacity decay rate of 0.001% per cycle over 350 cycles at 2C). And further optimization suggests that the suitable treatment on CFs could balance the chemical adsorption (OFGs) and physical confinement (carbon cages), leading to fast and durable electrochemical reaction dynamics. In addition, the assembled soft-pack LSBs further show a high dynamic bending stability.

6.
Front Neurosci ; 15: 755056, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34658782

RESUMO

Uric acid (URIC) is a natural antioxidant, and it has been shown that low levels of URIC could be a risk factor for the development of Parkinson's disease. Our aim was to investigate whether URIC also plays a role in Meige's syndrome (MS). We conducted a cohort study to compare serum URIC levels between patients with MS and healthy controls. In addition, we analyzed the impact of URIC on the risk of MS and symptom severity. Compared with normal subjects, URIC content was remarkably decreased in MS patients. In addition, URIC was regarded as a protective factor for MS, as verified by multivariate logistic regression models. We also found non-linear relationships between the levels of serum URIC and the incidence rate of MS and the Burke-Fahn-Marsden dystonia rating scale score. Our study is the first to show a connection between serum URIC levels and MS. Low serum URIC levels indicate an increased risk of MS incidence and more severe clinical symptoms. Our findings provide new insights into the prevention and treatment of MS.

7.
Int J Biol Macromol ; 188: 450-459, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34371041

RESUMO

The bacterial type VI secretion system (T6SS) is a powerful arsenal that fires many toxic effectors into neighboring cells to gain advantage over inter-bacterial competition and eukaryotic host infection. Meanwhile, the cognate immunity proteins of these effectors are employed to protect themselves from the virulence. TseT-TsiT is a newly discovered effector-immunity (E-I) protein pair secreted by T6SS of Pseudomonas aeruginosa. Our group had reported the crystal structure of TsiT before. Here, we report the crystal structure of P. aeruginosa TseT-TsiT complex at 3.1 Å resolution. The interface of TseT-TsiT is characterized in this work. Through structure and small angle X-ray scattering (SAXS) studies, we discover that the long C-terminal helix of TseT may be flexible. Combining the homolog comparison results, we propose that TseT may form an oligomer in favor of its putative nuclease activity. Although TsiT doesn't directly block the putative active-site of TseT, it may hinder the TseT's oligomerization process to neutralize its virulence.

8.
Am J Epidemiol ; 2021 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-34240101

RESUMO

Ogburn et al. (Am J Epidemiol. 2021;000(0):000-000) raise a cautionary tale for epidemiological data fusion: bias may occur if a variable completely missing in the primary dataset is imputed according to a regression model estimated from an auxiliary dataset. However, in some specific settings, solution may exist. Focusing on a linear outcome regression model with a missing covariate, we show that the bias can be eliminated if the underlying imputation model for the missing covariate is nonlinear in the common variables measured in both datasets. Otherwise, we describe two alternative approaches existing in the data fusion literature that could partially resolve this issue: one estimates the outcome model by leveraging an additional validation dataset containing joint observations of the outcome and the missing covariate, and the other offers informative bounds for the outcome regression coefficients without using validation data. We justify these three methods on a linear outcome model and briefly discuss their extension to general settings.

9.
Acta Crystallogr A Found Adv ; 77(Pt 4): 277-288, 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-34196290

RESUMO

A new multi-lattice indexing method based on the principle of whole-pattern matching given cell dimensions and space-group symmetry is presented for macromolecular crystallography. The proposed method, termed the multi-crystal data processing suite (MCDPS), features a local correction for prior information accompanied by iterative refinement of experimental parameters, both of which are numerically and experimentally demonstrated to be critical for accurately identifying multiple crystal lattices. Further analysis of data reduction and structure determination with conventional single-crystal programs reveals that the processed multi-lattice data sets are comparable in quality to typical single-crystal ones in terms of crystallographic metrics. Importantly, it is confirmed that careful exclusion of overlapping reflections prior to scaling is necessary to guarantee an accurate data reduction result. The potential for multi-lattice indexing in solving the general macroscopic twinning problem is also explored.

10.
J Struct Biol ; 213(3): 107770, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34303831

RESUMO

Electron tomography, a powerful imaging tool for studying 3D structures of macromolecular assemblies, always suffers from imperfect reconstruction with limited resolution due to the intrinsic low signal-to-noise ratio (SNR) and inaccessibility to certain tilt angles induced by radiation damage or mechanical limitation. In order to compensate for such insufficient data with low SNR and further improve imaging resolution, prior knowledge constraints about the objects in both real space and reciprocal space are thus exploited during tomographic reconstruction. However, direct Fast Fourier transform (FFT) between real space and reciprocal space remains extraordinarily challenging owing to their inconsistent grid sampling modes, e.g. regular and uniform grid sampling in real space whereas radial or polar grid sampling in reciprocal space. In order to solve such problem, a technique of non-uniform fast Fourier transform (NFFT) has been developed to transform efficiently between non-uniformly sampled grids in real and reciprocal space with sufficient accuracy. In this work, a Non-Uniform fast Fourier transform based Dual-space constraint Iterative reconstruction Method (NUDIM) applicable to biological electron tomography is proposed with a combination of basic concepts from equally sloped tomography (EST) and NFFT based reconstruction. In NUDIM, the use of NFFT can circumvent such grid sampling inconsistency and thus alleviate the stringent equally-sloped sampling requirement in EST reconstruction, while the dual-space constraint iterative procedure can dramatically enhance reconstruction quality. In comparison with conventional reconstruction methods, NUDIM is numerically and experimentally demonstrated to produce superior reconstruction quality with higher contrast, less noise and reduced missing wedge artifacts. More importantly, it is also capable of retrieving part of missing information from a limited number of projections.

12.
Biomed Res Int ; 2021: 5567046, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33959658

RESUMO

Stroke is the main cause of acquired epilepsy in elderly people. Poststroke epilepsy (PSE) not only affects functional recovery after stroke but also brings considerable social consequences. While some factors such as cortical involvement, hemorrhagic transformation, and stroke severity are associated with increased seizure risk, so far that remains controversial. In recent years, there are an increasing number of studies on potential biomarkers of PSE as tools for diagnosing and predicting epileptic seizures. Biomarkers such as interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), glutamate, and S100 calcium-binding protein B (S100B) in blood are associated with the occurrence of PSE. This review is aimed at summarizing the progress on potential biomarkers of PSE.


Assuntos
Biomarcadores/sangue , Epilepsia , Acidente Vascular Cerebral/complicações , Epilepsia/sangue , Epilepsia/diagnóstico , Epilepsia/etiologia , Ácido Glutâmico/sangue , Humanos , Interleucina-6/sangue , Subunidade beta da Proteína Ligante de Cálcio S100/sangue , Fator de Necrose Tumoral alfa/sangue
13.
Anal Chem ; 93(20): 7405-7412, 2021 05 25.
Artigo em Inglês | MEDLINE | ID: mdl-33973465

RESUMO

Extracellular vesicles (EVs) play a significant role in the pathophysiological process of many diseases, highlighting their values in medical diagnosis and disease monitoring. However, the current EV isolation methods are time-consuming, inconvenient to operate, and incompatible with downstream analyses. Here, we present a novel isolation method employing anionic polysaccharide-modified filter papers for the isolation of EVs (AppiEV) via electrostatic adsorption. A disc of glass fiber-based filter modified with sodium alginate was assembled into a spin column to function as the solid capture phase. In the acidic condition, EVs were induced to carry more positively charged ions, which enable the capture of EVs by the negatively charged filter paper. After a wash, the EVs were released from the spin column using an alkaline elution buffer, which induces the EVs to carry more negative charges. The EVs isolated by AppiEV from cell culture supernatants, plasma, and urine are similar to or even better than those isolated by ultracentrifugation in terms of EV size distribution, protein distribution, and nucleic acid contents. Due to the interference removal of the EV-free RNA and DNA attributed to the negatively charged capture medium, the eluate of AppiEV could be directly used for genetic analysis, including the stem-loop RT-PCR analysis of miR-21 and the allele-specific PCR analysis of mutation genes of EGFR p.L858R and EGFR p.T790M. We believe that AppiEV offers a simple and efficient approach for the isolation of high-quality EVs from various liquid specimens.


Assuntos
Vesículas Extracelulares , Neoplasias Pulmonares , Receptores ErbB , Humanos , Mutação , Polissacarídeos , Inibidores de Proteínas Quinases
14.
Int J Biol Sci ; 17(5): 1277-1288, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33867845

RESUMO

The angiotensin-converting enzyme 2 (ACE2) receptor has been identified as the cell entry point for SARS-CoV-2. Although ACE2 receptors are present in the bone marrow, the effects of SARS-CoV-2 on the biological activity of bone tissue have not yet been elucidated. In the present study we sought to investigate the impact of SARS-CoV-2 on osteoblastic activity in the context of fracture healing. MicroRNA-4485 (miR-4485), which we found to be upregulated in COVID-19 patients, negatively regulates osteogenic differentiation. We demonstrate this effect both in vitro and in vivo. Moreover, we identified the toll-like receptor 4 (TLR-4) as the potential target gene of miR-4485, and showed that reduction of TLR-4 induced by miR-4485 suppresses osteoblastic differentiation in vitro. Taken together, our findings highlight that up-regulation of miR-4485 is responsible for the suppression of osteogenic differentiation in COVID-19 patients, and TLR-4 is the potential target through which miR-4485 acts, providing a promising target for pro-fracture-healing and anti-osteoporosis therapy in COVID-19 patients.


Assuntos
COVID-19/patologia , Diferenciação Celular , Consolidação da Fratura , MicroRNAs/metabolismo , Osteogênese , SARS-CoV-2/fisiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Enzima de Conversão de Angiotensina 2/metabolismo , COVID-19/virologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , SARS-CoV-2/isolamento & purificação , Receptor 4 Toll-Like/metabolismo
15.
Stat Med ; 40(17): 3975-3989, 2021 07 30.
Artigo em Inglês | MEDLINE | ID: mdl-33902164

RESUMO

Mediation analysis is a useful tool in randomized trials for understanding how a treatment works, in particular how much of the treatment's effect on an outcome is explained by a mediator variable. The traditional approach to mediation analysis makes sequential ignorability assumption which precludes the existence of unobserved confounders between the mediator and outcome variables. Since the randomized experiment does not randomize the mediator, sequential ignorability may not be plausible. In this article, based on a statistical model termed sure outcomes of random events model, we propose an alternative approach to causal mediation analysis without relying on the sequential ignorability assumption for the case of binary treatment and mediator variables. When the outcome is also binary, we establish the identifiability of the average natural direct and indirect effects in the presence of an unobserved confounder between mediator and outcome variables. More importantly, if the identifiability conditions are violated, we provide new bounds that are narrower than those in the previous studies, and these bound results are extended to the case of an arbitrary bounded outcome. Simulation studies show good performance for the proposed estimators in finite samples. Finally, we use a job training intervention on the mental health study to illustrate our approach.


Assuntos
Análise de Mediação , Modelos Estatísticos , Simulação por Computador , Humanos
16.
J Trauma Acute Care Surg ; 91(5): 841-848, 2021 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-33901052

RESUMO

BACKGROUND: Damage-control resuscitation (DCR) improves survival in severely bleeding patients. However, deviating from balanced transfusion ratios during a resuscitation may limit this benefit. We hypothesized that maintaining a balanced resuscitation during DCR is independently associated with improved survival. METHODS: This was a secondary analysis of the Prospective Observational Multicenter Major Trauma Transfusion (PROMMTT) study. Patients receiving >3 U of packed red blood cells (PRBCs) during any 1-hour period over the first 6 hours and surviving beyond 30 minutes were included. Linear regression assessed the effect of percent time in a high-ratio range on 24-hour survival. We identified an optimal ratio and percent of time above the target ratio threshold by Youden's index. We compared patients with a 6-hour ratio above the target and above the percent time threshold (on-target) with all others (off-target). Kaplan-Meier analysis assessed the combined effect of blood product ratio and percent time over the target ratio on 24-hour and 30-day survival. Multivariable logistic regression identified factors independently associated with 24-hour and 30-day survival. RESULTS: Of 1,245 PROMMTT patients, 524 met the inclusion criteria. Optimal targets were plasma/PRBC and platelet/PRBC of 0.75 (3:4) and ≥40% time spent over this threshold. For plasma/PRBC, on-target (n = 213) versus off-target (n = 311) patients were younger (median, 31 years; interquartile range, [22-50] vs. 40 [25-54]; p = 0.002) with similar injury burdens and presenting physiology. Similar patterns were observed for platelet/PRBC on-target (n = 116) and off-target (n = 408) patients. After adjusting for differences, on-target plasma/PRBC patients had significantly improved 24-hour (odds ratio, 2.25; 95% confidence interval, 1.20-4.23) and 30-day (odds ratio, 1.97; 95% confidence interval, 1.14-3.41) survival, while on-target platelet/PRBC patients did not. CONCLUSION: Maintaining a high ratio of plasma/PRBC during DCR is independently associated with improved survival. Performance improvement efforts and prospective studies should capture time spent in a high-ratio range. LEVEL OF EVIDENCE: Epidemiologic/prognostic study, level II; Therapeutic, level IV.


Assuntos
Transfusão de Sangue/estatística & dados numéricos , Hemorragia/terapia , Ressuscitação/estatística & dados numéricos , Ferimentos não Penetrantes/terapia , Adulto , Transfusão de Sangue/métodos , Transfusão de Sangue/normas , Feminino , Hemorragia/etiologia , Hemorragia/mortalidade , Mortalidade Hospitalar , Humanos , Escala de Gravidade do Ferimento , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Guias de Prática Clínica como Assunto , Estudos Prospectivos , Ressuscitação/métodos , Ressuscitação/normas , Estudos Retrospectivos , Resultado do Tratamento , Ferimentos não Penetrantes/complicações , Ferimentos não Penetrantes/diagnóstico , Ferimentos não Penetrantes/mortalidade , Adulto Jovem
17.
FEBS J ; 288(19): 5755-5767, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-33838074

RESUMO

The bacterial type VI secretion system (T6SS) secretes many toxic effectors to gain advantage in inter-bacterial competition and for eukaryotic host infection. The cognate immunity proteins of these effectors protect bacteria from the virulence of their own effectors. The T6SS injects its inner-needle Hcp tube, the sharpening tip complex -consisting of VgrG and proline-alanine-alanine-arginine repeats (PAAR) proteins- and toxic effectors into neighboring cells. Its functions are largely determined by the activities of its delivered effectors. Five PAAR proteins were found in the Pseudomonas aeruginosa PAO1 genome with three of them shown to facilitate the delivery of various effectors. Here, we report a putative virus-type replication-repair nuclease domain-containing effector TseV encoded by the least investigated P. aeruginosa PAAR2 cluster. The crystal structure of its putative cognate effector TsiV is presented at 1.6 Å resolution. Through structure and sequence comparisons, we propose TseV-TsiV to be a putative novel effector-immunity (E-I) pair and we discuss the roles of other PAAR2 cluster encoded proteins.


Assuntos
Infecções Bacterianas/genética , Proteínas de Bactérias/genética , Pseudomonas aeruginosa/genética , Sistemas de Secreção Tipo VI/genética , Sequência de Aminoácidos/genética , Infecções Bacterianas/microbiologia , Genoma Bacteriano/genética , Interações Hospedeiro-Patógeno/genética , Humanos
18.
World J Clin Cases ; 9(8): 1946-1952, 2021 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-33748246

RESUMO

BACKGROUND: Accidental dural puncture (ADP) and subsequent post-dural puncture headache (PDPH) remain common complications of epidural procedures for obstetric anesthesia and analgesia. No clear consensus exists on the best way to prevent PDPH after ADP. CASE SUMMARY: We report our findings in twenty parturients who underwent an incorporated strategy of epidural analgesia followed by epidural hydroxyethyl starch (HES) to prevent PDPH after ADP with a 16-gauge Tuohy needle during epidural procedures. ADP with a 16-gauge Tuohy needle occurred in nine parturients undergoing a cesarean section (CS) and in eleven parturients receiving labor analgesia. An epidural catheter was re-sited at the same or adjacent intervertebral space in all patients. After CS, the epidural catheter was used for postoperative pain relief over a 48-h period. After delivery in eleven cases, epidural infusion was maintained for 24 h. Thereafter, 15 mL of 6% HES 130/0.4 was administered via the epidural catheter immediately prior to catheter removal. None of the parturients developed PDPH or neurologic deficits over a follow-up period of at least two months to up to one year postpartum. CONCLUSION: An incorporated strategy of epidural analgesia followed by epidural hydroxyethyl starch may have great efficacy in preventing PDPH after ADP.

19.
Artigo em Inglês | MEDLINE | ID: mdl-33626572

RESUMO

BACKGROUND: Congenital heart disease (CHD) accounts for the most common birth defects in China, pressuring both the physical and mental health in children. The inaccessibility of CHD children in rural China due to financial difficulties is demanding inputs from both the government and society. The Heartguard project is a program developed to improve the delivery of CHD care in rural China. METHODS: The Heartguard project partners with county hospitals and performs CHD screening to diagnose patients with CHD in rural China. Diagnosed children with CHD who are unable to afford therapy will subsequently receive treatment sponsored by the financial partners. All patients are followed up by the local partner and visiting surgical team members. RESULTS: More than 10,000 children across 9 provinces underwent CHD screening. A total of 240 (accounting for an incidence of 2.4%) was treated by the program, of which 226 patients were managed invasively, the other 14 patients conservatively. Open surgery was performed in 162 patients, while endovascular procedures were applied in another 64. No mortality or significant complications occurred during the transfer. There was no perioperative or late death. CONCLUSION: This humanitarian cardiac surgery program is able to promote accessibility of care for CHD children in rural China. The quality of life of these patients can be improved with continuous input from the society.

20.
ACS Appl Mater Interfaces ; 13(7): 8967-8975, 2021 Feb 24.
Artigo em Inglês | MEDLINE | ID: mdl-33576595

RESUMO

The internal concentration polarization (ICP) of asymmetric osmotic membranes with support layers greatly reduced membrane water permeability, therefore compromising membrane performance. In this study, a series of free-standing symmetric hybrid forward osmosis (FO) membranes without experiencing ICP were fabricated by covalently linking metal-organic framework (MOF) nanofillers with a polymer matrix. Owing to the introduction of MOFs, which allow only water permeation but reject salts by steric hindrance, the prepared hybrid membranes could approach the empirical permeability-selectivity trade-off. The optimized hybrid membrane displayed an outstanding water/Na2SO4 selectivity of ∼1208.4 L mol-1, compared with that of conventional membranes of ∼375.6 L mol-1. Additionally, the fabricated hybrid membranes showed excellent mechanical robustness, maintaining structural integrity during the long-term FO separation of high-salinity solution. This work provides an effective methodology to fabricate high-performance, symmetric MOF-based membranes for osmotic separation processes such as seawater desalination and water purification.

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