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1.
Braz Oral Res ; 35: e29, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33605359

RESUMO

This study evaluated how Er,Cr:YSGG laser, associated or not with 5% fluoride varnish, influences the surface roughness and volume loss of bovine root dentin submitted to erosive and/or abrasive wear. One hundred and twenty dentin specimens were divided into the groups: without preventive treatment (WPT), 5% fluoride varnish (FV); Er,Cr:YSGG laser irradiation (L), and varnish combined with laser (FV + L). The specimens (n = 10) were subdivided into: 1 = erosion (E); 2 = abrasion (A); and 3 = erosion followed by abrasion (E + A). The erosive solution used was a soft-drink (pH = 2.42 at 4ºC) applied in 5-min cycles twice a day for 10d. Abrasive wear involved brushing for 60s with an electric brush (1,600-oscillations/s) at a load of 2.0N. Surface roughness and volume loss were evaluated using a laser scanning confocal microscope. Roughness data were submitted to one-way ANOVA and Tukey post-hoc test. For volume loss, the Kruskal-Wallis and Dunn's post-hoc tests were used (α = 5%). The lowest values of roughness were found in the control areas of all subgroups (p > 0.05). In the experimental area, the [(WPT) + (E+A)] subgroup had a significantly higher roughness (5.712 ± 0.163 µm 2 ) than the other subgroups (p < 0.05). The L and (FV + L) groups had statistically similar roughness, regardless of the type of wear. The (FV + L) group had the lowest volume loss, regardless of the type of wear performed: [(FV + L) + (E)] = 7.5%, [(FV + L) + (A) = 7.3%, and [(FV + L) + (E + A)] = 8.1%. The subgroup [(WPT) + (E + A)] had the highest volume loss (52.3%). The proposed treatments were effective in controlling dentin roughness. Laser irradiation can be an effective method to increase root dentin resistance after challenges and limit problems related to non-carious lesions.


Assuntos
Lasers de Estado Sólido , Erosão Dentária , Animais , Bovinos , Dentina , Fluoretos , Lasers de Estado Sólido/uso terapêutico , Erosão Dentária/etiologia , Raiz Dentária , Escovação Dentária
2.
Braz. oral res. (Online) ; 35: e29, 2021. tab, graf
Artigo em Inglês | LILACS-Express | LILACS, BBO - Odontologia | ID: biblio-1153613

RESUMO

Abstract This study evaluated how Er,Cr:YSGG laser, associated or not with 5% fluoride varnish, influences the surface roughness and volume loss of bovine root dentin submitted to erosive and/or abrasive wear. One hundred and twenty dentin specimens were divided into the groups: without preventive treatment (WPT), 5% fluoride varnish (FV); Er,Cr:YSGG laser irradiation (L), and varnish combined with laser (FV + L). The specimens (n = 10) were subdivided into: 1 = erosion (E); 2 = abrasion (A); and 3 = erosion followed by abrasion (E + A). The erosive solution used was a soft-drink (pH = 2.42 at 4ºC) applied in 5-min cycles twice a day for 10d. Abrasive wear involved brushing for 60s with an electric brush (1,600-oscillations/s) at a load of 2.0N. Surface roughness and volume loss were evaluated using a laser scanning confocal microscope. Roughness data were submitted to one-way ANOVA and Tukey post-hoc test. For volume loss, the Kruskal-Wallis and Dunn's post-hoc tests were used (α = 5%). The lowest values of roughness were found in the control areas of all subgroups (p > 0.05). In the experimental area, the [(WPT) + (E+A)] subgroup had a significantly higher roughness (5.712 ± 0.163 μm 2 ) than the other subgroups (p < 0.05). The L and (FV + L) groups had statistically similar roughness, regardless of the type of wear. The (FV + L) group had the lowest volume loss, regardless of the type of wear performed: [(FV + L) + (E)] = 7.5%, [(FV + L) + (A) = 7.3%, and [(FV + L) + (E + A)] = 8.1%. The subgroup [(WPT) + (E + A)] had the highest volume loss (52.3%). The proposed treatments were effective in controlling dentin roughness. Laser irradiation can be an effective method to increase root dentin resistance after challenges and limit problems related to non-carious lesions.

3.
J. health sci. (Londrina) ; 21(4): https://seer.pgsskroton.com/index.php/JHealthSci/issue/view/399, 20/12/2019.
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1051674

RESUMO

The objective of this in vitro study was to evaluate the influence of the Er,Cr:YSGG laser associated or not to a desensitizing agent in the treatment of erosive lesions. Forty specimens with dimensions of 4mm x 4mm and 3mm thickness were divided into 4 groups (n = 10): G1- no treatment; G2- 5% fluoride varnish; G3- Er,Cr: YSGG; G4 - fluoride varnish + laser. The specimens were immersed in erosive drink 3 times a day for 1 minute with an average interval of 2 hours between cycles for a period of 10 days. The treatments were performed according to the groups and the surface roughness and the wear profile were analyzed by scanning confocal microscopy. The normality (Kolmogorov-Smirnov) and homogeneity (Levene's) of the tests were evaluated. After these analyzes, the surface roughness data were submitted to the statistical analysis of Variance Analysis (ANOVA). All tests adopted a significance level of 5% (α = 0.05). At the representative images of the wear profile, the morphology of bovine dental enamel in its control and eroded areas were compared and qualitatively discussed. As regard surface roughness, there was no statistically significant difference between the groups. The qualitative analysis of the loss of volume showed that all experimental groups showed significant superficial morphology differences. Considering the limitations of an in vitro study, it can be concluded that the treatments performed were not able to treat dental erosion satisfactorily, indicating the need for more treatment sessions. (AU)


O objetivo desse estudo in vitro foi avaliar a influência do laser Er,Cr:YSGG associado ou não a um agente dessensibilizante no tratamento de lesões erosivas. Foram confeccionados 40 espécimes com dimensões de 4mm x 4mm e 3mm de espessura, divididos em 4 grupos (n=10): G1- nenhum tratamento; G2- verniz fluoretado 5%; G3- aplicação do laser Er,Cr:YSGG; G4- verniz fluoretado + laser. Os espécimes foram imersos em bebida erosiva, 3 vezes por dia, durante 1 minuto, com intervalo médio de 2 horas entre os ciclos, por um período de 10 dias. Os tratamentos foram realizados de acordo com os grupos e que foram analisados a rugosidade superficial e o perfil de desgaste por meio de microscopia confocal de varredura. Avaliou-se a normalidade (Kolmogorov-Smirnov) e homogeneidade (Levene's) dos dados. Após estas análises, os dados de rugosidade superficial foram submetidos ao teste estatístico de Análise de Variância (ANOVA). Todos os testes adotaram nível de significância de 5% (α=0,05). Através da obtenção das imagens representativas do perfil desgaste, a morfologia do esmalte dental bovino em suas áreas controle e erodida foram comparadas e qualitativamente discutidas. Quanto à rugosidade superficial, não houve diferença estatisticamente significativa entre os grupos. A análise qualitativa da perda de volume mostrou que todos os grupos experimentais apresentaram diferenças significativas na morfologia superficial. Considerando as limitações de um estudo in vitro pode-se concluir que os tratamentos realizados não foram capazes de tratar a erosão dentária de forma satisfatória, indicando a necessidade de mais sessões de tratamento.

4.
J. health sci. (Londrina) ; 21(2): https://seer.pgsskroton.com/index.php/JHealthSci/article/view/6448, 19/06/2019.
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1051277

RESUMO

Early diagnosis of white spot lesions allows non-invasive treatment to be indicated for the remineralization of active lesions. The goal was to report the clinical Protocol for the treatment of white spot lesion of a patient by the technique of resin infiltration, observing whether there is change of color on the white spot of the dental enamel after its treatment. The selected patient was 16 years old with good overall health, who featured white spot lesion of the dental enamel on tooth 13, classified as ICDAS score 2. The tooth was photographed, and its color was evaluated with the spectrophotometer in accordance with the CIELhC system. For comparison purposes, the color of tooth enamel in an area adjacent to the lesion was evaluated. The treatment of the lesion was performed with the resin infiltrate Icon (DMG, Hamburg, Germany), following the manufacturer's instructions. The color of the tooth was re-evaluated after the application of the resin infiltrate. At the end of the procedure, it was observed that the treatment of white spot lesion by resin infiltration technique reached its goal because, in addition to being minimally invasive, it was highly approved by patient and it provided the masking of the white spot lesion on the dental enamel. (AU).


O diagnóstico precoce de lesões de mancha branca permite que um tratamento não invasivo seja indicado para a remineralização das lesões ativas. O objetivo foi relatar o protocolo clínico para tratamento de lesão de mancha branca ativa de um paciente pela técnica do infiltrante resinoso, observando se existe alteração de cor da mancha branca do esmalte dental após o seu tratamento. Foi selecionada uma paciente, de 16 anos de idade, com boa saúde geral e que apresentava lesão de mancha branca no esmalte dental do dente 13, classificada pelo escore 2 do ICDAS. O dente foi fotografado e sua cor foi avaliada com o espectrofotômetro de acordo com o sistema CIELhC. Para fins de comparação, foi avaliada a cor do esmalte dental em uma área adjacente à lesão. O tratamento da lesão foi realizado com a resina infiltrante Icon (DMG, Hamburgo, Alemanha), seguindo as recomendações do fabricante. A cor do dente foi reavaliada após a aplicação do infiltrante. Ao final do procedimento, observou-se que o tratamento da lesão de mancha branca pela técnica da resina infiltrante atingiu seu objetivo pois, além de ter sido minimamente invasiva, foi bem avaliada pela paciente e proporcionou o mascaramento da lesão de mancha branca do esmalte dental. (AU).

5.
Einstein (Sao Paulo) ; 17(1): eAO4515, 2019 Jan 31.
Artigo em Inglês, Português | MEDLINE | ID: mdl-30726310

RESUMO

OBJECTIVE: To detect Streptococcus mutans in colostrum and saliva of neonates and compare with its detection in saliva of mothers. METHODS: Forty-three healthy women, full-term gestations with no complications, submitted to elective Cesarean section, and their newborns were included in the study. Samples were investigated by polymerase chain reaction to detect S. mutans in genetic material from the samples. RESULTS: Approximately 16% of colostrum samples showed S. mutans , but not correlated with the presence of the bacteria in both samples of saliva. S. mutans was detected in 49 and 30% of saliva samples of mothers and neonates, respectively. There was a positive correlation in S. mutans detection between types of saliva. The number of maternal samples of saliva with detectable S. mutans was smaller in women receiving dental treatment during pregnancy. Tooth brushing, three times a day, influenced the detection of S. mutans in both the saliva and the colostrum. CONCLUSION: Although maternal saliva may present S. mutans , few samples of colostrum present the bacteria. The presence of bacteria in neonate saliva may be related to contact before birth. Dental treatment and hygiene habits seem to influence the detection of S. mutans in samples of maternal saliva and colostrum.


Assuntos
Colostro/microbiologia , Saúde Bucal/estatística & dados numéricos , Saliva/microbiologia , Streptococcus mutans/isolamento & purificação , Brasil , Cesárea , Feminino , Humanos , Recém-Nascido , Transmissão Vertical de Doença Infecciosa/estatística & dados numéricos , Reação em Cadeia da Polimerase , Gravidez
6.
Lasers Med Sci ; 34(5): 893-900, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30374705

RESUMO

This in vitro study evaluated the influence of the Er,Cr:YSGG laser, associated or not to desensitizing agents, in the prevention of acid erosion in bovine root dentin. Eighty dentin specimens were selected and divided into eight groups (n = 10): G1: negative control; G2: positive control (5% fluoride varnish-FV); G3: Er,Cr:YSGG laser; G4: FV + laser; G5: 3% potassium oxalate; G6: 3% potassium oxalate + laser; G7: biphasic calcium silicate/phosphate gel (gel); G8: gel + laser. Laser parameters: 0.5 W, 6.25 J/cm2 at 1-mm distance. The erosive drink used was a cola soft-drink (pH = 2.42 at 4 °C), lasting 5 min, twice a day, with 6-h intervals between the challenges, during 14 days. Kolmogorov-Smirnov and Levene's tests were satisfied. The surface roughness data were submitted to ANOVA and Tukey post hoc tests. For the wear profile, Kruskal-Wallis and Dunn post hoc tests were used. Afterwards, the Spearman correlation test was performed. All statistical tests assumed a significance level of 5% (α = 0.05). G1 presented the highest surface roughness value after the erosive challenge (3.586 µm2 ± 0.205 µm2) and the G7 presented the lowest surface roughness value after the erosive challenge (1.071 µm2 ± 0.180 µm2). For the lost volume, G4 presented the lowest percentage (9.7% ± 0.9%), while G1 had the highest percentage (41.8% ± 2.5%), both with p < 0.05. There was a weak correlation between the response variables (ρ = 0.33). All groups presented lower values of surface roughness and loss of volume when compared to the negative control group. For the surface roughness, the biphasic calcium silicate/phosphate gel presented the best result. For volume loss, the 5% fluoride varnish + Er,Cr:YSGG laser showed the best results compared to the other groups.


Assuntos
Ácidos/efeitos adversos , Dessensibilizantes Dentinários/farmacologia , Dentina/efeitos da radiação , Lasers de Estado Sólido , Erosão Dentária/prevenção & controle , Raiz Dentária/efeitos da radiação , Animais , Bovinos , Dentina/efeitos dos fármacos , Estatísticas não Paramétricas , Propriedades de Superfície , Raiz Dentária/efeitos dos fármacos
7.
Einstein (Säo Paulo) ; 17(1): eAO4515, 2019. tab
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-984368

RESUMO

ABSTRACT Objective To detect Streptococcus mutans in colostrum and saliva of neonates and compare with its detection in saliva of mothers. Methods Forty-three healthy women, full-term gestations with no complications, submitted to elective Cesarean section, and their newborns were included in the study. Samples were investigated by polymerase chain reaction to detect S. mutans in genetic material from the samples. Results Approximately 16% of colostrum samples showed S. mutans , but not correlated with the presence of the bacteria in both samples of saliva. S. mutans was detected in 49 and 30% of saliva samples of mothers and neonates, respectively. There was a positive correlation in S. mutans detection between types of saliva. The number of maternal samples of saliva with detectable S. mutans was smaller in women receiving dental treatment during pregnancy. Tooth brushing, three times a day, influenced the detection of S. mutans in both the saliva and the colostrum. Conclusion Although maternal saliva may present S. mutans , few samples of colostrum present the bacteria. The presence of bacteria in neonate saliva may be related to contact before birth. Dental treatment and hygiene habits seem to influence the detection of S. mutans in samples of maternal saliva and colostrum.


RESUMO Objetivo Detectar Streptococcus mutans no colostro e na saliva de recém-nascido, e comparar com a detecção na saliva da mãe. Métodos Foram incluídos no estudo 43 mulheres saudáveis, com gestações a termo e sem complicações, que tiveram cesariana eletiva, e seus recém-nascidos. As amostras foram investigadas por reação de polimerase em cadeia para a detecção de S. mutans em material genético extraído das amostras. Resultados Cerca de 16% das amostras de colostro apresentaram S. mutans , não sem correlação com a presença das bactérias em ambas amostras de saliva. S. mutans foi detectado em 49 e 30% das amostras de saliva das mães e recém-nascidos, respectivamente. Houve correlação positiva na detecção de S. mutans entre os tipos de saliva. O número de amostras de saliva materna com S. mutans detectável foi menor nas mulheres que receberam tratamento odontológico durante a gravidez. A escovação três vezes ao dia influenciou na detecção do S. mutans tanto no saliva quanto no colostro. Conclusão Embora a saliva materna possa apresentar S. mutans , poucas amostras de colostro apresentam a bactéria. A presença de bactéria na saliva de neonatos pode estar relacionada ao contato antes do nascimento. O tratamento odontológico e os hábitos de higiene parecem influenciar na detecção de S. mutans em amostras de saliva e colostro maternos.

8.
J. health sci. (Londrina) ; 20(4)30/12/2018.
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-970569

RESUMO

The objective of this in vitro study was to evaluate the influence of different remineralizing agents in the treatment of enamel erosive lesions. Specimens of 4mmx4mm and 3mm thickness were made from the buccal surface of bovine incisors (n=10) and randomly divided into 4 groups. G1 = application of the remineralizing dentifrice, G2 = application of the remineralizing agent, G3 = remineralizing dentifrice + remineralizing agente, G4 = application of fluoride varnish (positive control), G5 = no treatment Specimens were immersed in refrigerant solution during a period of 10 days. The surface roughness was analyzed by means of confocal laser scanning microscopy. The data were analyzed for homogeneity (Levene's) and normality (Kolmogorov-Smirnov). Parametric tests with analysis of variance were performed on two criteria: time factor and treatment factor, and Tukey post-test for differentiation of means. All tests were statistically significant at 5% (α = 0.05). The results showed statistically significant difference, demonstrating the reduction of surface roughness after the first treatment (G3) and the other groups (G1, G2 and G4) only after the second treatment. It was concluded that the use of dentifrice composed of calcium silicate and sodium phosphate influenced the roughness of the eroded tooth enamel of the bovine tooth. (AU)


O objetivo deste trabalho in vitro foi avaliar a influência de diferentes agentes remineralizantes no tratamento de lesões erosivas em esmalte. Foram confeccionados espécimes de 4mmx4mm e 3 mm de espessura a partir da superfície vestibular de incisivos bovinos (n=10) e divididos aleatoriamente em 4 grupos. G1=aplicação do dentifrício remineralizante, G2= aplicação do agente potencializador remineralizante, G3= dentifrício remineralizante + agente potencializador remineralizante, G4=aplicação de verniz fluoretado (controle positivo), G5=nenhum tratamento (controle negativo). Os espécimes foram imersos em refrigerante durante um período de 10 dias. A rugosidade superficial foi analisada por meio de microscopia confocal de varredura a laser. Os dados foram analisados quanto à homogeneidade (Levene's) e normalidade (Kolmogorov- Smirnov). Foram realizados testes paramétricos com análise de variância a dois critérios: fator tempo e fator tratamento, e pós-teste de Tukey para diferenciação das médias. Todos os testes estatísticos tiveram nível de significância de 5% (α=0,05). Os resultados obtidos mostraram diferenças estatisticamente significantes, demonstrando a redução da rugosidade da superfície do esmalte logo após o primeiro tratamento (G3) e para os demais grupos (G1, G2 e G4) somente após o segundo tratamento. Concluiu-se que a utilização de dentifrício composto por silicato de cálcio e fosfato de sódio influenciou na rugosidade do esmalte erodido do dente bovino. (AU).

9.
J. health sci. (Londrina) ; 20(3)31/10/2018.
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-965541

RESUMO

A resina composta é o material restaurador mais utilizado atualmente. Apesar de seu sucesso clínico, o material está sujeito a alterações em suas propriedades físicas, quando exposto ao meio bucal. O objetivo foi avaliar a influência da degradação química na estabilidade de cor e na rugosidade superficial de resinas micro-híbrida e nanoparticulada. Foram confeccionados 40 discos (6,0mm x 2,0mm) da resina composta micro-híbrida (Z250XT) e 40 da resina nanoparticulada (Z350XT). Após a análise da cor inicial e da rugosidade inicial, 10 discos de cada resina foram imersos em saliva artificial (Controle, pH=6,4), suco de laranja (pH= 3,4), refrigerante de limão (pH=2,9) e vinho tinto (pH=3,1), por 4 horas por dia (37ºC) durante 30 dias. Após, foram realizadas as leituras finais de cor e rugosidade. Os dados obtidos para a estabilidade de cor foram analisados pelo Teste de Kruskal-Wallis, seguido pelo Teste de Dunn (α=5%). Para a análise da rugosidade foi utilizado o Teste t para amostras pareadas (α=5%). O manchamento provocado pela saliva artificial, suco de laranja e refrigerante foi similar, mas foi estatisticamente diferente do manchamento produzido pelo vinho, em ambas as resinas testadas. O vinho produziu o mesmo grau de manchamento nas resinas micro-híbrida e nanoparticulada. Foi observado que o suco de laranja, o refrigerante e o vinho promoveram aumento da rugosidade superficial dos discos de resina. Diante disso, concluiu-se que o vinho alterou a cor de resinas compostas micro-híbrida e nanoparticulada, e que a rugosidade superficial dos discos de resina foi alterada por todas as bebidas utilizadas. (AU).


Composite resin is the most used restorative material. Despite its clinical success, it is susceptible to changes in its physical properties when exposed to the oral environment. The aim was to evaluate the influence of acidic beverages on the color stability and on the surface roughness of the nanofilled and micro hybrid composite. Forty discs (6.0mm x 2.0mm) of the micro hybrid composite (Z250XT) and 40 of the nanofilled composite (Z350XT) were manufactured. After the measurement of the initial color and the initial roughness, 10 discs of each composite were individually immersed in artificial saliva (control, pH = 6.4), orange juice (pH=3.4), lime soda (pH = 2.9) and red wine (pH = 3.1) during 4 hours per day at 37°C for 30 days. At the end of the experiment, the final analysis of color and surface roughness were performed. The data obtained for the color stability were analyzed by Kruskal-Wallis and Dunn's test (α= 5%). For the analysis of the surface roughness, the t test for related samples was used (α= 5%). The color changes promoted by artificial saliva, orange juice and soda were similar, but they were statistically different from staining produced by wine, in both tested composites. Wine produced the same degree of staining in both composites. Orange juice, soda and red wine increased the surface roughness of the composite discs. It was concluded that red wine significantly changes the color of micro hybrid and nanofilled composites. Furthermore, all beverages increased the surface roughness of the composites tested in the study herein. (AU).

10.
Restor Dent Endod ; 43(3): e32, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30135851

RESUMO

Objectives: This study evaluated the effects of a bleaching agent on the composition, mechanical properties, and surface topography of 6 conventional glass-ionomer cements (GICs) and one resin-modified GIC. Materials and Methods: For 3 days, the specimens were subjected to three 20-minute applications of a 37% H2O2-based bleaching agent and evaluated for water uptake (WTK), weight loss (WL), compressive strength (CS), and Knoop hardness number (KHN). Changes in surface topography and chemical element distribution were also analyzed by energy-dispersive X-ray spectroscopy and scanning electron microscopy. For statistical evaluation, the Kruskal-Wallis and Wilcoxon paired tests (α = 0.05) were used to evaluate WTK and WL. CS specimens were subjected to 2-way analysis of variance (ANOVA) and the Tukey post hoc test (α = 0.05), and KH was evaluated by one-way ANOVA, the Holm-Sidak post hoc test (α = 0.05), and the t-test for independent samples (α = 0.05). Results: The bleaching agent increased the WTK of Maxxion R, but did not affect the WL of any GICs. It had various effects on the CS, KHN, surface topography, and the chemical element distribution of the GICs. Conclusions: The bleaching agent with 37% H2O2 affected the mechanical and surface properties of GICs. The extent of the changes seemed to be dependent on exposure time and cement composition.

11.
ROBRAC ; 27(83): 252-256, out./dez. 2018. ilus
Artigo em Português | LILACS-Express | LILACS | ID: biblio-997249

RESUMO

O diagnóstico precoce de lesões de mancha branca no esmalte dental permite que um tratamento não invasivo seja indicado para a remineralização das lesões ativas. O objetivo deste trabalho foi relatar um caso clínico onde foi utilizado um infiltrante resinoso para o tratamento não invasivo de uma lesão de mancha branca do esmalte dental. Foi selecionado um paciente com 14 anos de idade, do sexo masculino, leucoderma, com boa saúde geral, de alto risco à cárie, que apresentava lesão de mancha branca no incisivo central superior direito. Devido ao aspecto rugoso e opaco da lesão, optou-se pela realização do tratamento do dente com uma resina infiltrante (Icon®, DMG, Hamburg, Alemanha). Inicialmente foram realizadas fotografias intraorais e radiografia do dente acometido para fins de documentação do caso e futuras comparações. Em seguida, o dente recebeu profilaxia e posterior tratamento pela técnica da resina infiltrante, de acordo com as instruções do fabricante. Para a verificação da efetividade do procedimento, o paciente foi avaliado imediatamente e após 2 meses da conclusão do tratamento. Concluiu-se que a técnica da resina infiltrante de baixa viscosidade para o tratamento da lesão de mancha branca do dente 11 foi satisfatória, pois foi capaz de promover a paralização da doença e o mascaramento da lesão de mancha branca naquele dente.


The early diagnosis of enamel white spot lesions allows adopting a non-invasive treatment to remineralize active lesions. The objective of this study was to report a case where an infiltrant composite was used to treat enamel white spot lesion. A Caucasian 14- year-old male patient, with overall good health, and high-risk for dental caries was selected. He presented white spot lesion in the upper right central incisor. Due to the rough and opaque appearance of the lesion, the treatment with an infiltrant resin (Icon®, DMG, Hamburg, Germany) was chosen. Initially, intraoral photos and x-ray of the affected tooth have been were taken for documentation purposes and future comparisons. Then, the tooth was cleaned and treated with the infiltrant resin following the manufacturer's instructions. To verify the effectiveness of the procedure, the patient was assessed immediately and 2 months after treatment. In conclusion, the technique of low-viscosity infiltrant resin was satisfactory because it was able to impair the progression of the disease and to camouflage the white spot lesion on that tooth.

12.
Curr Microbiol ; 75(10): 1372-1377, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29934881

RESUMO

The aims of this study were to analyze the presence of Streptococcus mutans (SM)-DNA in cord blood (CB), maternal peripheral blood (PB), and maternal saliva (SA) and compare with data collected in health surveys. Sixty-four healthy women with pregnancies to term and without complications attending for elective cesarean section in the Clinical Hospital of Ribeirao Preto, Sao Paulo were included. Samples of PB and unstimulated SA were obtained on the day of hospitalization and samples of CB were collected after the delivery section. Samples were investigated using polymerase chain reaction for the presence of SM-DNA using specific primers. The results show over 50% of the sample of PB and CB showed SM-DNA detectable. There was a positive correlation between the SM detection in PB/CB and SA (P < 0.05). Pregnant women, who reported tooth brushing more than three times a day, often showed detectable SM-DNA in PB and CB (P < 0.05). In conclusion, the majority of children can have contact with SM-DNA during the intrauterine life by the CB. SM probably transferred from salivary habitat to PB and CB. The tooth brushing can be associated to S. mutans detection in blood samples.


Assuntos
Ácidos Nucleicos Livres , DNA Bacteriano , Sangue Fetal , Saliva , Infecções Estreptocócicas/sangue , Infecções Estreptocócicas/microbiologia , Streptococcus mutans/genética , Adulto , Brasil/epidemiologia , Feminino , Humanos , Mães , Gravidez , Vigilância em Saúde Pública , Fatores de Risco , Infecções Estreptocócicas/epidemiologia , Adulto Jovem
13.
Einstein (Sao Paulo) ; 15(3): 256-261, 2017.
Artigo em Inglês, Português | MEDLINE | ID: mdl-29091144

RESUMO

OBJECTIVE: To describe e compare the specificity of IgA antibodies against bacteria extract of Klebsiella pneumoniae , Staphylococcus aureus , Escherichia coli , and Salmonella enteritidis . METHODS: Colostrum samples were aseptically collected in the first 12 hours after C-section delivery. The specificity of IgA against bacteria extracts was analyzed by the Western blot. RESULTS: The findings showed proteins of high molecular weight frequently detectable in the samples. S. aureus was the most frequently found bacterium in the samples (p<0.05). Approximately 93.8, 56.3, 62.5 and 60.4% of samples presented IgA reactive to S. aureus , K. pneumoniae , S. enteritidis, and E. coli, respectively. Roughly 40% of samples showed no IgA reactive to K. pneumoniae, S. enteritidis and E. coli . CONCLUSION: Clinical evidence of the importance of breastfeeding for the immune protection of neonates was consistent with the observed immunological findings, since most samples showed IgA reactive against the species tested. The application and development of immunotherapies during pregnancy, focused on frequently detected antigens, could be an important tool to enhance the presence of IgA in colostrum.


Assuntos
Anticorpos Antibacterianos/análise , Colostro/imunologia , Escherichia coli/imunologia , Imunoglobulina A Secretora/análise , Klebsiella pneumoniae/imunologia , Salmonella enteritidis/imunologia , Staphylococcus aureus/imunologia , Anticorpos Antibacterianos/imunologia , Western Blotting , Feminino , Humanos , Imunoglobulina A Secretora/imunologia , Recém-Nascido , Sensibilidade e Especificidade
14.
Einstein (Säo Paulo) ; 15(3): 256-261, July-Sept. 2017. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-891403

RESUMO

ABSTRACT Objective To describe e compare the specificity of IgA antibodies against bacteria extract of Klebsiella pneumoniae , Staphylococcus aureus , Escherichia coli , and Salmonella enteritidis . Methods Colostrum samples were aseptically collected in the first 12 hours after C-section delivery. The specificity of IgA against bacteria extracts was analyzed by the Western blot. Results The findings showed proteins of high molecular weight frequently detectable in the samples. S. aureus was the most frequently found bacterium in the samples (p<0.05). Approximately 93.8, 56.3, 62.5 and 60.4% of samples presented IgA reactive to S. aureus , K. pneumoniae , S. enteritidis, and E. coli, respectively. Roughly 40% of samples showed no IgA reactive to K. pneumoniae, S. enteritidis and E. coli . Conclusion Clinical evidence of the importance of breastfeeding for the immune protection of neonates was consistent with the observed immunological findings, since most samples showed IgA reactive against the species tested. The application and development of immunotherapies during pregnancy, focused on frequently detected antigens, could be an important tool to enhance the presence of IgA in colostrum.


RESUMO Objetivo Descrever e comparar a especificidade de anticorpos IgA de amostras de colostro contra extratos bacterianos de Klebsiella pneumoniae , Staphylococcus aureus , Escherichia coli e Salmonella enteritidis . Métodos As amostras de colostro foram coletadas assepticamente nas primeiras 12 horas após o nascimento por cesariana. A especificidade de IgA contra extratos de bactérias foi analisada por Western blot. Resultados Os achados mostraram proteínas de alto peso molecular frequentemente detectáveis nas amostras. S. aureus foi a bactéria mais encontrada nas amostras (p<0,05). Cerca de 93,8, 56,3, 62,5 e 60,4% das amostras apresentaram IgA reativa a S. aureus , K. pneumoniae , S. enteritidis e E. coli , respectivamente. Aproximadamente 40% das amostras não apresentaram IgA reativa contra K. pneumoniae , S. enteritidis e E. coli. Conclusão A evidência clínica da importância da amamentação para proteção imunológica ao recém-nascido foi consistente com os achados imunológicos observados, uma vez que a maioria das amostras mostrou IgA reativa contra as espécies testadas. A aplicação e o desenvolvimento de imunoterapias durante a gestação, focada nos antígenos frequentemente detectados, poderiam ser importantes instrumentos para aumentar a presença de IgA no colostro.

15.
Braz Oral Res ; 31: e39, 2017 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-28513788

RESUMO

The present study compared IgA specificity against oral streptococci in colostrum and saliva samples. Sixty-two mother-and-child pairs were included; samples of colostrum (C) and saliva (MS) were collected from the mothers and saliva samples were collected from babies (BS). The specificity of IgA against Streptococcus mutans and S. mitis were analyzed by western blot. Only 30% of babies' samples presented IgA reactivity to S. mutans, while 74 and 80% of MS and C, respectively, presented this response. IgA reactivity to S. mutans virulence antigens (Ag I/II, Gtf and GbpB) in positive samples showed differences between samples for Gtf and especially for GbpB (p < 0.05), but responses to Ag I/II were similar (p > 0.05). The positive response of Gtf-reactive IgA was different between C (90%) and MS (58%) samples (p < 0.05), but did not differ from BS (p > 0.05). GbpB was the least detected, with 48 and 26% of C and MS, and only 5% of BS samples presenting reactivity (p > 0.05). Eight percent of MS and C samples presented identical bands to SM in the same time-point. In conclusion, the differences of IgA response found between C and MS can be due to the different ways of stimulation, proliferation and transportation of IgA in those secretions. The colostrum has high levels of IgA against S. mutans virulence antigens, which could affect the installation and accumulation process of S. mutans, mainly by supplying anti-GbpB IgA to the neonate.


Assuntos
Colostro/imunologia , Imunoglobulina A Secretora/análise , Imunoglobulina A Secretora/imunologia , Saliva/imunologia , Streptococcus mitis/imunologia , Streptococcus mutans/imunologia , Análise de Variância , Formação de Anticorpos/imunologia , Antígenos de Bactérias/análise , Antígenos de Bactérias/imunologia , Proteínas de Bactérias/análise , Proteínas de Bactérias/imunologia , Western Blotting , Colostro/microbiologia , Ensaio de Imunoadsorção Enzimática , Feminino , Glucosiltransferases/análise , Glucosiltransferases/imunologia , Glicoproteínas/análise , Glicoproteínas/imunologia , Humanos , Recém-Nascido , Mães , Saliva/microbiologia , Virulência
16.
Braz. oral res. (Online) ; 31: e39, 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-839507

RESUMO

Abstract The present study compared IgA specificity against oral streptococci in colostrum and saliva samples. Sixty-two mother-and-child pairs were included; samples of colostrum (C) and saliva (MS) were collected from the mothers and saliva samples were collected from babies (BS). The specificity of IgA against Streptococcus mutans and S. mitis were analyzed by western blot. Only 30% of babies’ samples presented IgA reactivity to S. mutans, while 74 and 80% of MS and C, respectively, presented this response. IgA reactivity to S. mutans virulence antigens (Ag I/II, Gtf and GbpB) in positive samples showed differences between samples for Gtf and especially for GbpB (p < 0.05), but responses to Ag I/II were similar (p > 0.05). The positive response of Gtf-reactive IgA was different between C (90%) and MS (58%) samples (p < 0.05), but did not differ from BS (p > 0.05). GbpB was the least detected, with 48 and 26% of C and MS, and only 5% of BS samples presenting reactivity (p > 0.05). Eight percent of MS and C samples presented identical bands to SM in the same time-point. In conclusion, the differences of IgA response found between C and MS can be due to the different ways of stimulation, proliferation and transportation of IgA in those secretions. The colostrum has high levels of IgA against S. mutans virulence antigens, which could affect the installation and accumulation process of S. mutans, mainly by supplying anti-GbpB IgA to the neonate.


Assuntos
Humanos , Feminino , Recém-Nascido , Saliva/imunologia , Streptococcus mutans/imunologia , Imunoglobulina A Secretora/análise , Imunoglobulina A Secretora/imunologia , Colostro/imunologia , Streptococcus mitis/imunologia , Saliva/microbiologia , Proteínas de Bactérias/análise , Proteínas de Bactérias/imunologia , Virulência , Ensaio de Imunoadsorção Enzimática , Glicoproteínas/análise , Glicoproteínas/imunologia , Western Blotting , Análise de Variância , Colostro/microbiologia , Glucosiltransferases/análise , Glucosiltransferases/imunologia , Mães , Formação de Anticorpos/imunologia , Antígenos de Bactérias/análise , Antígenos de Bactérias/imunologia
17.
Braz Oral Res ; 30(1): e111, 2016 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-27737364

RESUMO

The objective of this study was to evaluate the effects of diode laser and a desensitizing dentifrice on dentin permeability. Fifty-two root dentin fragments were obtained (5 × 5mm) and treated with 24% EDTA gel. The samples were divided into 4 groups (n = 13): G1, control (no treatment); G2, diode laser (λ = 908 nm, 1.5 W, continuous mode, 20s); G3, application of abrasive dentifrice for 1 minute (Elmex Sensitive Professional (International Gaba); and G4, application of abrasive dentifrice for 1 minute followed by irradiation with diode laser. Ten samples per group were immersed in 2% methylene blue solution for 4h. The specimens were washed, longitudinally sectioned, observed under optical microscopy, photographed and assessed based on the degree of dye leakage. The remaining samples were observed under scanning electron microscopy (SEM). The leakage data were subjected to ANOVA test, followed by Tukey's t-test (α = 5%). Groups 2, 3 and 4 showed less dye penetration than the control group (p < 0.05), but were similar among each other. SEM images showed that dentinal tubules were open in G1, and fused and occluded in G2. Group 3 showed dentinal tubules that were occluded by the metal ions from the toothpaste. G4 presented similar characteristics to G3, and the presence of fused dentin. The diode laser and the dentifrice were effective in reducing dentinal permeability, and the combination of the two treatments did not show better results than either one used alone.


Assuntos
Dentifrícios/química , Dessensibilizantes Dentinários/química , Permeabilidade da Dentina/efeitos dos fármacos , Sensibilidade da Dentina/tratamento farmacológico , Dentina/efeitos dos fármacos , Raiz Dentária/efeitos dos fármacos , Animais , Bovinos , Dentina/efeitos da radiação , Permeabilidade da Dentina/efeitos da radiação , Terapia a Laser/métodos , Lasers Semicondutores/uso terapêutico , Microscopia Eletrônica de Varredura , Distribuição Aleatória , Reprodutibilidade dos Testes , Propriedades de Superfície/efeitos dos fármacos , Propriedades de Superfície/efeitos da radiação , Fatores de Tempo , Raiz Dentária/efeitos da radiação
18.
J. health sci. (Londrina) ; 18(3): 210-216, 31/07/2016.
Artigo em Português | LILACS-Express | LILACS | ID: biblio-831809

RESUMO

A hipersensibilidade dentinária - HD é caracterizada por uma dor aguda, de curta duração, causada pela movimentação de fluidos no interior de túbulos dentinários expostos ao meio bucal. Recentemente, a utilização dos lasers de alta e baixa intensidade foi proposta como uma alternativa para o tratamento da HD. Este trabalho realizou uma revisão da literatura baseada em artigos publicados entre 2009 e 2016 sobre os efeitos dos lasers de baixa e alta intensidade para o tratamento da HD. A base de dados utilizada foi o PubMed utilizando-se os descritores: Dentin hypersensitivity, laser, Nd:YAG, Er:YAG, GaAlAs, Er,Cr:YSGG, CO2 , HeNe, e hydrodinamic theory. Foi observado que o tratamento da HD com lasers é um método biocompatível, não-invasivo e eficiente. Os lasers de baixa intensidade (HeNe e GaAlAs) estimulam as células nervosas do tecido pulpar, interferindo na polaridade das membranas celulares, pelo aumento da amplitude do potencial de ação. Esta ação leva ao bloqueio da transmissão do estímulo ao sistema nervoso central e estimula os odontoblastos a produzirem dentina reparadora, o que diminui a permeabilidade da dentina e interrompe a movimentação de fluidos nos túbulos dentinários. Os lasers em alta intensidade (Nd:YAG, Er:YAG, Er,Cr:YSGG e CO2) têm o objetivo de obliterar a embocadura dos túbulos dentinários, através da irradiação direta da dentina exposta ao meio bucal, promovendo a dissolução e a ressolidificação dos cristais de hidroxiapatita da dentina, levando à eliminação da sensibilidade dolorosa por um tempo prolongado. Pode-se concluir que os lasers de alta e baixa intensidade são efetivos para o tratamento da hipersensibilidade dentinária cervical.


Dentin hypersensitivity - DH is characterized by an acute and short-duration pain caused by the movement of fluids into the dentinal tubules that are exposed to the oral environment. Recently, the use of lasers has been proposed as an alternative for the treatment of DH. This study conducted a literature review based on papers published between 2009 and 2016 about the use of low and high intensity lasers for the treatment of HD. The database PubMed was consulted, using the following keywords: dentin hypersensitivity, laser, Nd : YAG, Er:YAG, GaAlAs, Er, Cr:YSGG, CO2 , HeNe, and hydrodinamic theory. It was observed that the treatment of DH with lasers can be a biocompatible, non-invasive, and efficient method. The low-intensity lasers (HeNe and GaAlAs) stimulate nerve cells in the pulp tissue, interfering with the polarity of the cell membranes by increasing the amplitude of the action potential, that inhibits the transmission of stimuli to the central nervous system. It stimulates the odontoblasts to produce reparative dentine, which decreases dentin permeability and impairs the movement of fluids into the dentinal tubules. The high-intensity lasers (Nd:YAG, Er:YAG, Er,Cr:YSGG and CO2) obliterate the dentinal tubules by direct irradiation of dentin that is exposed to the oral environment, promoting the dissolution and resolidification of hydroxyapatite crystals, leading to elimination of pain sensitivity for a long period. It can be concluded that the high and low intensity lasers are effective for the treatment of cervical dentine hypersensitivity

19.
Braz. oral res. (Online) ; 30(1): e111, 2016. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-951996

RESUMO

Abstract The objective of this study was to evaluate the effects of diode laser and a desensitizing dentifrice on dentin permeability. Fifty-two root dentin fragments were obtained (5 × 5mm) and treated with 24% EDTA gel. The samples were divided into 4 groups (n = 13): G1, control (no treatment); G2, diode laser (λ = 908 nm, 1.5 W, continuous mode, 20s); G3, application of abrasive dentifrice for 1 minute (Elmex Sensitive Professional (International Gaba); and G4, application of abrasive dentifrice for 1 minute followed by irradiation with diode laser. Ten samples per group were immersed in 2% methylene blue solution for 4h. The specimens were washed, longitudinally sectioned, observed under optical microscopy, photographed and assessed based on the degree of dye leakage. The remaining samples were observed under scanning electron microscopy (SEM). The leakage data were subjected to ANOVA test, followed by Tukey's t-test (α = 5%). Groups 2, 3 and 4 showed less dye penetration than the control group (p < 0.05), but were similar among each other. SEM images showed that dentinal tubules were open in G1, and fused and occluded in G2. Group 3 showed dentinal tubules that were occluded by the metal ions from the toothpaste. G4 presented similar characteristics to G3, and the presence of fused dentin. The diode laser and the dentifrice were effective in reducing dentinal permeability, and the combination of the two treatments did not show better results than either one used alone.

20.
Arch Oral Biol ; 60(1): 116-25, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25290442

RESUMO

OBJECTIVES: Secretory immunoglobulins present in mucosa surfaces represent the first line of defense of the adaptive immune system against infectious challenges. Preterm (PT) neonates' humoral immunity is diminished compared to full-term (FT) newborns. The identification of important antigens (Ags) of virulence of oral species may help in the investigation of the mechanisms of antigenic stimulation and the development of the mucosal immune response. In the present study, we measured saliva levels of immunoglobulins A (IgA) and M (IgM) and characterized the specificity of IgA against Ags of several streptococcal species found early in life. METHODS: This was a prospective observational study. Salivary IgA (sIgA) antibody responses to bacterial species that are prototypes of pioneer (Streptococcus mitis, S. sanguinis, S. gordonii) and pathogenic (Streptococcus mutans) microorganisms of the oral cavity were studied in FT and PT children in two visits: at birth (T0) and at 3 months of age (T3). Salivas from 123 infants (72 FT and 51 PT) were collected during the first 10h after birth (T0) and again at 3 months of age (T3). Salivary levels of IgA and IgM antibodies were analysed by enzyme-linked immunosorbent assay (ELISA). A subgroup of 26 FT and 24 PT children were compared with respect to patterns of antibody specificities against different streptococci Ags using Western blot assays. RESULTS: No significant differences (P>0.05) in salivary levels of IgA and IgM between FT and PT babies were found at birth. At T3, mean sIgA values were similar between groups and sIgM levels were significantly higher in PT than FT (P<0.05). Western blot assays identified positive IgA response to streptococci in the majority of children, especially in the FT group. There were some differences between groups in relation to the frequency of children with positive response to Ags and intensity of IgA response. In general, oral streptococci Ags were more frequently detected and bands were more intense in FT than in PT, especially in T3. Prospective analysis of patterns of sIgA against Ags of different streptococcal species revealed an increase in complexity of the sIgA antibody response from the first day of birth (T0) to T3 in PT and FT. CONCLUSION: The patterns of sIgA response to streptococci Ags appear to be influenced by the gestational age, which might reflect the level of immunological maturity of the mucosal immune system.


Assuntos
Formação de Anticorpos/imunologia , Saliva/imunologia , Streptococcus/imunologia , Western Blotting , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imunoglobulina A Secretora/imunologia , Imunoglobulina M/imunologia , Lactente , Recém-Nascido , Recém-Nascido Prematuro/imunologia , Masculino , Estudos Prospectivos , Proteínas/imunologia , Saliva/química , Saliva/microbiologia
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